Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1873 183.1 9376148 98.2% 9207377 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,091,351 C→T G168D (GGC→GAC)  rhaD ← rhamnulose‑phosphate aldolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,091,3510CT100.0% 739.8 / NA 204G168D (GGC→GAC) rhaDrhamnulose‑phosphate aldolase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (87/117);  total (87/117)

Only 100 of 217 total aligned reads displayed.

GCGGTCGCCTGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGCCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGTGCATGATC  >  NC_000913/4091261‑4091440
                                                                                          |                                                                                         
gCGGTCGCCTGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCt                                                                                         <  2:4392420/93‑1 (MQ=255)
gCGGTCGCCTGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCt                                                                                         >  2:4020941/1‑93 (MQ=255)
gCGGTCGCCTGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCt                                                                                         <  2:1698060/93‑1 (MQ=255)
  ggTCGCCTGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTc                                                                                       >  2:2485231/1‑93 (MQ=255)
  ggTCGCCTGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTCTCTTc                                                                                       >  2:3274427/1‑93 (MQ=255)
   gTCGCCTGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTcc                                                                                      <  1:2757312/93‑1 (MQ=255)
   gTCGCCTGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTcc                                                                                      >  1:2526160/1‑93 (MQ=255)
      gCCTGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTccccc                                                                                   >  2:2414333/1‑91 (MQ=255)
      gCCTGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCcac                                                                                   >  1:3949348/1‑93 (MQ=255)
       ccTGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCcaca                                                                                  <  2:1651194/93‑1 (MQ=255)
           gCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTg                                                                              <  2:1583300/93‑1 (MQ=255)
            ccGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTgg                                                                             <  2:1422251/93‑1 (MQ=255)
            ccGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTgg                                                                             >  2:3465977/1‑93 (MQ=255)
             cGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGc                                                                            >  1:2261439/1‑93 (MQ=255)
             cGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGc                                                                            >  2:2085722/1‑93 (MQ=255)
             cGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGc                                                                            >  2:3017395/1‑93 (MQ=255)
                tttCTTCCGTGCCGAGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAg                                                                         <  2:1490471/93‑1 (MQ=255)
                tttCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAg                                                                         <  1:3690327/93‑1 (MQ=255)
                tttCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAg                                                                         >  1:704642/1‑93 (MQ=255)
                 ttCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCATCGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGt                                                                        <  2:4602250/93‑1 (MQ=255)
                 ttCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGt                                                                        <  1:3551718/93‑1 (MQ=255)
                 ttCGTCCGTACCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGt                                                                        <  2:1327336/93‑1 (MQ=255)
                    gTCCGTGCCGTGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGaa                                                                     <  1:2803048/93‑1 (MQ=255)
                    gTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGaa                                                                     <  1:3806620/93‑1 (MQ=255)
                     tCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAg                                                                    >  1:1001208/1‑93 (MQ=255)
                      ccGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGa                                                                   <  2:1858460/93‑1 (MQ=255)
                       cGTGCCGGTCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATCCCACCAGCCACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGCAGAc                                                                  <  2:1777971/93‑1 (MQ=255)
                       cGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGAc                                                                  <  2:827902/93‑1 (MQ=255)
                        gTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGAcc                                                                 >  1:3478044/1‑93 (MQ=255)
                        gTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGAcc                                                                 <  1:3865815/93‑1 (MQ=255)
                          gCCGGGCCCCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACcgc                                                               <  1:2420816/93‑1 (MQ=255)
                          gCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACcgc                                                               <  1:2391475/93‑1 (MQ=255)
                          gCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACcgc                                                               <  1:2080512/93‑1 (MQ=255)
                          gCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACcgc                                                               <  1:343041/93‑1 (MQ=255)
                            cGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCgg                                                             <  2:4469478/93‑1 (MQ=255)
                               gCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGgtgt                                                          <  2:890250/93‑1 (MQ=255)
                               gCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGgtgc                                                          >  1:3625799/1‑92 (MQ=255)
                                 aCCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCg                                                        >  2:513970/1‑93 (MQ=255)
                                 aCCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCg                                                        >  2:936855/1‑93 (MQ=255)
                                  ccATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGt                                                       >  1:2904693/1‑93 (MQ=255)
                                   cATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGtt                                                      <  1:2574678/93‑1 (MQ=255)
                                   cATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGtt                                                      >  1:3169855/1‑93 (MQ=255)
                                   cATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGtt                                                      <  1:1036466/93‑1 (MQ=255)
                                   cATCCACGGCAAAATGCCAACCCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGtt                                                      <  2:115505/93‑1 (MQ=255)
                                    aTCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGttt                                                     <  1:3596587/93‑1 (MQ=255)
                                     tCCGCGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGtttt                                                    <  1:657278/93‑1 (MQ=255)
                                     tCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGtttt                                                    <  2:2133102/93‑1 (MQ=255)
                                     tCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGtttt                                                    <  1:2341026/93‑1 (MQ=255)
                                     tCCACGCCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGtttt                                                    <  2:4454685/93‑1 (MQ=255)
                                      ccACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTc                                                   >  1:107382/1‑93 (MQ=255)
                                      ccACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTc                                                   <  2:281848/93‑1 (MQ=255)
                                       cACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGCGTCGTTTTCa                                                  >  2:2302681/1‑93 (MQ=255)
                                        aCGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCaa                                                 <  1:735193/93‑1 (MQ=255)
                                        aCGACAAAATGCCACCGCCATCCGGGAATACCACCAGACTCTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCaa                                                 <  2:4127820/93‑1 (MQ=255)
                                             aaaaTGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACa                                            <  1:172866/93‑1 (MQ=255)
                                               aaTGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATa                                          <  1:529555/93‑1 (MQ=255)
                                                aTGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAg                                         >  1:3339771/1‑93 (MQ=255)
                                                    cAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGAACGCGGTGTCGTTTTCAAGTACATAGGTGa                                     >  1:3989786/1‑93 (MQ=255)
                                                      aCGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAgg                                   >  2:957139/1‑93 (MQ=255)
                                                      aCGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAgg                                   <  1:4273293/93‑1 (MQ=255)
                                                      aCGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAgg                                   <  2:66853/93‑1 (MQ=255)
                                                        gCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGc                                 <  1:3035483/93‑1 (MQ=255)
                                                         ccATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCg                                >  1:1319553/1‑93 (MQ=255)
                                                         ccATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCg                                <  1:285569/93‑1 (MQ=255)
                                                         ccATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCg                                >  2:2567386/1‑93 (MQ=255)
                                                          cATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGa                               >  1:1629652/1‑93 (MQ=255)
                                                            tCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATc                             >  1:1869208/1‑93 (MQ=255)
                                                            tCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATc                             <  2:2437170/93‑1 (MQ=255)
                                                             ccGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCa                            >  1:115447/1‑93 (MQ=255)
                                                              cGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAg                           <  2:3394834/93‑1 (MQ=255)
                                                              cGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAg                           >  2:2248700/1‑93 (MQ=255)
                                                               gggAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAgg                          <  1:4466668/93‑1 (MQ=255)
                                                                ggAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGt                         <  1:4430087/93‑1 (MQ=255)
                                                                ggAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGt                         <  1:3245675/93‑1 (MQ=255)
                                                                  aaTACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTtg                       <  1:2827805/93‑1 (MQ=255)
                                                                   aTACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTtgg                      <  2:2740867/93‑1 (MQ=255)
                                                                    tACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTtggt                     >  1:4315396/1‑93 (MQ=255)
                                                                    tACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTtggt                     <  1:4181451/93‑1 (MQ=255)
                                                                    tACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTtggt                     <  1:40450/93‑1 (MQ=255)
                                                                     accaccAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTtggtg                    <  1:4205769/93‑1 (MQ=255)
                                                                      ccaccaGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTtggtgg                   >  2:2151133/1‑93 (MQ=255)
                                                                       caccaGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGgtggc                  <  2:4680517/93‑1 (MQ=255)
                                                                         ccaGACTCTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGgtggcgt                <  2:4277593/93‑1 (MQ=255)
                                                                          caGACACTCGGTGCTGTCTCCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGgtggcgtg               <  1:1758415/93‑1 (MQ=255)
                                                                           aGACACTCTGTGCTGTCTTACCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGgtggcgtgg              >  2:4552162/1‑93 (MQ=255)
                                                                            gACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGgtggcgtggc             >  1:3838877/1‑93 (MQ=255)
                                                                            gACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGgtggcgtggc             >  2:3498863/1‑93 (MQ=255)
                                                                              cacTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAg           <  2:4541825/93‑1 (MQ=255)
                                                                              cacTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAg           <  1:1436955/93‑1 (MQ=255)
                                                                               acTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGt          >  1:3390220/1‑93 (MQ=255)
                                                                               acTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGt          >  1:1589150/1‑93 (MQ=255)
                                                                               acTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGt          <  1:4403811/93‑1 (MQ=255)
                                                                               acTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGt          <  2:2707183/93‑1 (MQ=255)
                                                                                cTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGTg         >  2:321349/1‑93 (MQ=255)
                                                                                cTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGTg         >  1:4383905/1‑93 (MQ=255)
                                                                                  cGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGTGCa       <  2:2588215/93‑1 (MQ=255)
                                                                                  cGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGTGCa       <  2:4166906/93‑1 (MQ=255)
                                                                                   ggTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGTGCAt      >  2:3625477/1‑93 (MQ=255)
                                                                                       ctgtCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGTGCATGATc  >  2:2043624/1‑93 (MQ=255)
                                                                                       ctgtCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGTGCATGATc  >  1:4633797/1‑93 (MQ=255)
                                                                                          |                                                                                         
GCGGTCGCCTGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGCCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGTGCATGATC  >  NC_000913/4091261‑4091440

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GTGCGGTCGCCTGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGCCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGTGCATGATCACCC  >  NC_000913/4091259‑4091444
                                                                                            |                                                                                             
GTGCGGTCGCCTGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTC                                                                                              >  SRR4302535.4151332/1‑94 (MQ=60)
  GCGGTCGCCTGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTT                                                                                            <  SRR4302535.3480306/94‑1 (MQ=60)
    GGTCGCCTGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCC                                                                                          >  SRR4302535.2544359/1‑94 (MQ=60)
    GGTCGCCTGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCC                                                                                          >  SRR4302535.2158802/1‑94 (MQ=60)
     GTCGCCTGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCC                                                                                         >  SRR4302535.328731/1‑94 (MQ=60)
     GTCGCCTGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCC                                                                                         <  SRR4302535.2776961/94‑1 (MQ=60)
     GTCGCCTGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCC                                                                                         <  SRR4302535.1981842/94‑1 (MQ=60)
      TCGCCTGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCA                                                                                        <  SRR4302535.3827272/94‑1 (MQ=60)
       CGCCTGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCAC                                                                                       >  SRR4302535.3847624/1‑94 (MQ=60)
       CGCCTGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCAC                                                                                       >  SRR4302535.3976312/1‑94 (MQ=60)
         CCTGGGCGATTTCCGCCGCGCCGGGCCCCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAG                                                                                     <  SRR4302535.4082108/94‑1 (MQ=60)
          CTGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGT                                                                                    >  SRR4302535.1029547/1‑94 (MQ=60)
          CTGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGT                                                                                    <  SRR4302535.1591342/94‑1 (MQ=60)
             GCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGG                                                                                 <  SRR4302535.4618195/94‑1 (MQ=60)
              CCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGC                                                                                >  SRR4302535.2277549/1‑94 (MQ=60)
                GATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGA                                                                              >  SRR4302535.4071276/1‑94 (MQ=60)
                GATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCAGTGCTGTCTTCCCACAGTTGGCGA                                                                              >  SRR4302535.2604166/1‑94 (MQ=60)
                 ATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAG                                                                             >  SRR4302535.1500875/1‑94 (MQ=60)
                 ATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAG                                                                             >  SRR4302535.709590/1‑94 (MQ=60)
                  TTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGT                                                                            <  SRR4302535.4403751/94‑1 (MQ=60)
                  TTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGT                                                                            <  SRR4302535.3715869/94‑1 (MQ=60)
                   TTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTG                                                                           <  SRR4302535.3175614/94‑1 (MQ=60)
                   TTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTG                                                                           <  SRR4302535.3576365/94‑1 (MQ=60)
                      GTCCGTGCCGTGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAG                                                                        <  SRR4302535.2822982/94‑1 (MQ=60)
                      GTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAG                                                                        >  SRR4302535.1008322/1‑94 (MQ=60)
                      GTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAG                                                                        >  SRR4302535.680450/1‑94 (MQ=60)
                      GTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAG                                                                        <  SRR4302535.3832867/94‑1 (MQ=60)
                      GTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAG                                                                        >  SRR4302535.3370637/1‑94 (MQ=60)
                        CCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGAC                                                                      >  SRR4302535.3443820/1‑94 (MQ=60)
                         CGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACC                                                                     >  SRR4302535.3502213/1‑94 (MQ=60)
                          GTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCG                                                                    <  SRR4302535.3892401/94‑1 (MQ=60)
                            GCCGGGCCCCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCG                                                                  <  SRR4302535.2438247/94‑1 (MQ=60)
                            GCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCG                                                                  <  SRR4302535.2095502/94‑1 (MQ=60)
                            GCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCG                                                                  <  SRR4302535.345413/94‑1 (MQ=60)
                            GCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCG                                                                  <  SRR4302535.2408670/94‑1 (MQ=60)
                              CGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGT                                                                <  SRR4302535.2769929/94‑1 (MQ=60)
                                GGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGT                                                              <  SRR4302535.3869261/94‑1 (MQ=60)
                                GGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGC                                                              >  SRR4302535.3650927/1‑94 (MQ=60)
                                   ACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGT                                                           >  SRR4302535.2925226/1‑94 (MQ=60)
                                   ACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGT                                                           >  SRR4302535.4467402/1‑94 (MQ=60)
                                    CCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTT                                                          >  SRR4302535.3191809/1‑94 (MQ=60)
                                     CATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTT                                                         <  SRR4302535.2593183/94‑1 (MQ=60)
                                     CATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTT                                                         <  SRR4302535.1043826/94‑1 (MQ=60)
                                      ATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTT                                                        <  SRR4302535.3621546/94‑1 (MQ=60)
                                      ATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTT                                                        <  SRR4302535.2439365/94‑1 (MQ=60)
                                       TCCGCGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTC                                                       <  SRR4302535.661863/94‑1 (MQ=60)
                                       TCCACGGCAAAATGCCAACGCCCTCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTC                                                       >  SRR4302535.4679875/1‑94 (MQ=60)
                                       TCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTC                                                       <  SRR4302535.4440943/94‑1 (MQ=60)
                                       TCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTC                                                       >  SRR4302535.108142/1‑94 (MQ=60)
                                       TCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTC                                                       <  SRR4302535.4573388/94‑1 (MQ=60)
                                       TCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTC                                                       <  SRR4302535.2357772/94‑1 (MQ=60)
                                         CACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAA                                                     >  SRR4302535.171905/1‑94 (MQ=60)
                                          ACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAG                                                    <  SRR4302535.740362/94‑1 (MQ=60)
                                           CGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGT                                                   <  SRR4302535.1055539/94‑1 (MQ=60)
                                            GGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTA                                                  >  SRR4302535.2185899/1‑94 (MQ=60)
                                             GCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTAC                                                 <  SRR4302535.308249/94‑1 (MQ=60)
                                              CAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACA                                                >  SRR4302535.2877475/1‑94 (MQ=60)
                                              CAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACA                                                >  SRR4302535.25458/1‑94 (MQ=60)
                                               AAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACAT                                               <  SRR4302535.174062/94‑1 (MQ=60)
                                                AAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATA                                              <  SRR4302535.1426165/94‑1 (MQ=60)
                                                 AATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAG                                             >  SRR4302535.607753/1‑94 (MQ=60)
                                                 AATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAG                                             <  SRR4302535.533259/94‑1 (MQ=60)
                                                 AATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAG                                             >  SRR4302535.3363041/1‑94 (MQ=60)
                                                    GCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTG                                          <  SRR4302535.2404584/94‑1 (MQ=60)
                                                     CCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGAACGCGGTGTCGTTTTCAAGTACATAGGTGA                                         >  SRR4302535.4017075/1‑94 (MQ=60)
                                                      CAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAG                                        <  SRR4302535.3596110/94‑1 (MQ=60)
                                                       CACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGG                                       <  SRR4302535.4289364/94‑1 (MQ=60)
                                                       AACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGG                                       >  SRR4302535.2811758/1‑94 (MQ=60)
                                                        ACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGG                                      <  SRR4302535.717264/94‑1 (MQ=60)
                                                        ACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGG                                      <  SRR4302535.4302543/94‑1 (MQ=60)
                                                         CGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGG                                     >  SRR4302535.2426811/1‑94 (MQ=60)
                                                         CGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGC                                     <  SRR4302535.4224764/94‑1 (MQ=60)
                                                          GCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCG                                    >  SRR4302535.1328982/1‑94 (MQ=60)
                                                          GCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCG                                    <  SRR4302535.3056737/94‑1 (MQ=60)
                                                           CCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGA                                   >  SRR4302535.1641421/1‑94 (MQ=60)
                                                           CCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGA                                   <  SRR4302535.287566/94‑1 (MQ=60)
                                                            CATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGAT                                  <  SRR4302535.3645503/94‑1 (MQ=60)
                                                             ATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATC                                 >  SRR4302535.1882684/1‑94 (MQ=60)
                                                             ATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGCCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGAGCGATC                                 >  SRR4302535.3888496/1‑94 (MQ=60)
                                                              TCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCA                                <  SRR4302535.2850064/94‑1 (MQ=60)
                                                              TCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCA                                >  SRR4302535.116258/1‑94 (MQ=60)
                                                               CCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAG                               <  SRR4302535.1770382/94‑1 (MQ=60)
                                                                CGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGACGACCGCGGTGTCGTTTTCAAGTACATAGGAGAGGGCGATCAGG                              >  SRR4302535.2829554/1‑94 (MQ=60)
                                                                CGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGG                              <  SRR4302535.3990629/94‑1 (MQ=60)
                                                                 GGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGT                             <  SRR4302535.4497312/94‑1 (MQ=60)
                                                                  GGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTT                            <  SRR4302535.3519684/94‑1 (MQ=60)
                                                                  GGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTT                            <  SRR4302535.4460442/94‑1 (MQ=60)
                                                                  GGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTT                            <  SRR4302535.3268213/94‑1 (MQ=60)
                                                                    AATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGG                          <  SRR4302535.2308352/94‑1 (MQ=60)
                                                                    AATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGG                          <  SRR4302535.2847873/94‑1 (MQ=60)
                                                                     ATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGT                         >  SRR4302535.4344915/1‑94 (MQ=60)
                                                                     ATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGT                         <  SRR4302535.1774358/94‑1 (MQ=60)
                                                                     ATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGAGAGTGAAGACCGCGGTGTGGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGG                         >  SRR4302535.4250512/1‑94 (MQ=60)
                                                                      TACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTG                        <  SRR4302535.4210066/94‑1 (MQ=60)
                                                                      TACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTG                        <  SRR4302535.2975899/94‑1 (MQ=60)
                                                                      TACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTG                        <  SRR4302535.40757/94‑1 (MQ=60)
                                                                       ACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGG                       <  SRR4302535.4234551/94‑1 (MQ=60)
                                                                           CCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTG                   <  SRR4302535.433219/94‑1 (MQ=60)
                                                                            CAGACACTCGGTGCTGTCTCCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGG                  <  SRR4302535.1771126/94‑1 (MQ=60)
                                                                             AGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGC                 >  SRR4302535.3865338/1‑94 (MQ=60)
                                                                               ACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAG               <  SRR4302535.126665/94‑1 (MQ=60)
                                                                                CACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGT              >  SRR4302535.3413805/1‑94 (MQ=60)
                                                                                CACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGT              >  SRR4302535.1600618/1‑94 (MQ=60)
                                                                                CACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGT              <  SRR4302535.1447224/94‑1 (MQ=60)
                                                                                 ACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGTG             <  SRR4302535.1149550/94‑1 (MQ=60)
                                                                                 ACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGTG             >  SRR4302535.4413896/1‑94 (MQ=60)
                                                                                 ACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGTG             <  SRR4302535.4433948/94‑1 (MQ=60)
                                                                                   TCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGTGCA           >  SRR4302535.3901762/1‑94 (MQ=60)
                                                                                     GGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGTGCATG         >  SRR4302535.1620259/1‑94 (MQ=60)
                                                                                     GGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGTGCATG         <  SRR4302535.3252793/94‑1 (MQ=60)
                                                                                     GGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGTGCATG         <  SRR4302535.3684697/94‑1 (MQ=60)
                                                                                     GGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGTGCATG         <  SRR4302535.4417863/94‑1 (MQ=60)
                                                                                       TGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGTGCATGAT       >  SRR4302535.3563902/1‑94 (MQ=60)
                                                                                        GCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGTGCATGATC      >  SRR4302535.4665230/1‑94 (MQ=60)
                                                                                          TGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGTGCATGATCAC    >  SRR4302535.2709326/1‑94 (MQ=60)
                                                                                          TGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGTGCATGATCAC    <  SRR4302535.3178857/94‑1 (MQ=60)
                                                                                           GTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGCGGCGTGGCAGAGAATGATCACC   >  SRR4302535.2055358/1‑94 (MQ=60)
                                                                                            TCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGTGCATGATCACCC  <  SRR4302535.4041740/94‑1 (MQ=60)
                                                                                            |                                                                                             
GTGCGGTCGCCTGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGCCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGTGCATGATCACCC  >  NC_000913/4091259‑4091444

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑