Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1873 183.1 9376148 98.2% 9207377 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,198,118 A→G D87G (GAC→GGC)  hupA → DNA‑binding protein HU‑alpha

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,198,1180AG99.6% 822.7 / ‑5.1 261D87G (GAC→GGC) hupADNA‑binding protein HU‑alpha
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base G (114/145);  total (114/147)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.59e-01

Only 100 of 265 total aligned reads displayed.

AGCGTACTGGCCGCAACCCGCAGACCGGTAAAGAAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGACGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGC  >  NC_000913/4198028‑4198207
                                                                                          |                                                                                         
 gCGTACTGGCCGCAACCCGCAGACCGGTAAAGAAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGgcgc                                                                                        >  2:3394485/1‑93 (MQ=255)
    tACTGGCCGCAACCCGCAGACCGGTAAAGAAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGt                                                                                     >  1:2619694/1‑93 (MQ=255)
      cTGGCCGCAACCCGCAGACCGGTAAAGAAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTta                                                                                   >  2:2929065/1‑93 (MQ=255)
      cTGGCCGCAACCCGCAGACCGGTAAAGAAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTta                                                                                   >  1:1675368/1‑93 (MQ=255)
         gCCGCAACCCGCAGACCGGTAAAGAAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTtaagt                                                                                <  2:464444/93‑1 (MQ=255)
         gCCGCAACCCGCAGACCGGTAAAGAAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTtaaga                                                                                >  1:1294065/1‑92 (MQ=255)
           cGCAACCCGCAGACCGGTAAAGAAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTtaagtaa                                                                              <  1:4598806/93‑1 (MQ=255)
           cGCAACCCGCAGACCGGTAAAGAAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTtaagtaa                                                                              <  2:3774256/93‑1 (MQ=255)
           cGCAACCCGCAGACCGGTAAAGAAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGACGCAGTtaagtaa                                                                              <  2:3090799/93‑1 (MQ=255)
            gCAACCCGCAGACCGGTAAAGAAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTtaagtaag                                                                             >  2:2594949/1‑93 (MQ=255)
             cAACCCGCAGACCGGTAAAGAAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGa                                                                            <  1:1111551/93‑1 (MQ=255)
              aaCCCGCAGACCGGTAAAGAAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGAt                                                                           <  2:3224348/93‑1 (MQ=255)
              aaCCCGCAGACCGGTAAAGAAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGAt                                                                           <  2:4657305/93‑1 (MQ=255)
              aaCCCGCAGACCGGTAAAGAAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGAt                                                                           >  1:3246477/1‑93 (MQ=255)
               aCCCGCAGACCGGTAAAGAAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGAtt                                                                          <  1:26963/93‑1 (MQ=255)
                 ccGCAGACCGGTAAAGAAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGc                                                                        >  2:1710503/1‑93 (MQ=255)
                  cGCAGACCGGTAAAGAAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCg                                                                       <  2:3001847/93‑1 (MQ=255)
                   gCAGACCGGTAAAGAAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGt                                                                      <  2:3629680/93‑1 (MQ=255)
                     aGACCGGTAAAGAAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTgg                                                                    >  2:3551532/1‑93 (MQ=255)
                     aGACCGGTAAAGAAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTgg                                                                    >  2:2229933/1‑93 (MQ=255)
                        ccGGTAAAGAAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAg                                                                 <  2:4495750/93‑1 (MQ=255)
                         cGGTAAAGAAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGt                                                                <  2:3709589/93‑1 (MQ=255)
                         cGGTAAAGAAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGt                                                                >  2:3243299/1‑93 (MQ=255)
                          ggTAAAGAAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTg                                                               >  1:2317343/1‑93 (MQ=255)
                            tAAAGAAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGaa                                                             >  1:580892/1‑93 (MQ=255)
                              aaGAAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACa                                                           >  1:2930394/1‑93 (MQ=255)
                               aGAAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAg                                                          >  2:343397/1‑93 (MQ=255)
                               aGAAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAg                                                          <  1:2954701/93‑1 (MQ=255)
                                gAAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGt                                                         >  2:1893737/1‑93 (MQ=255)
                                gAAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGt                                                         <  2:4037458/93‑1 (MQ=255)
                                 aaaTCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGtt                                                        >  1:601893/1‑93 (MQ=255)
                                  aaTCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGttt                                                       <  2:117833/93‑1 (MQ=255)
                                   aTCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGtttt                                                      >  1:2205184/1‑93 (MQ=255)
                                      aaaaTTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAAc                                                   >  1:522130/1‑93 (MQ=255)
                                       aaaTTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACg                                                  >  1:4558772/1‑93 (MQ=255)
                                       aaaTTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACg                                                  <  1:3873824/93‑1 (MQ=255)
                                         aTTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGaa                                                <  1:3025824/93‑1 (MQ=255)
                                          ttGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAg                                               <  2:585144/93‑1 (MQ=255)
                                          ttGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAg                                               <  1:862460/93‑1 (MQ=255)
                                          ttGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAg                                               <  2:2481863/93‑1 (MQ=255)
                                          ttGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAg                                               >  1:3057457/1‑93 (MQ=255)
                                          ttGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAg                                               >  1:4136688/1‑93 (MQ=255)
                                          ttGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTGAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAg                                               <  1:1790115/93‑1 (MQ=255)
                                           tGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAgg                                              <  1:2783709/93‑1 (MQ=255)
                                           tGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAgg                                              <  2:3194890/93‑1 (MQ=255)
                                           tGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAgg                                              <  2:1636862/93‑1 (MQ=255)
                                           tGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAgg                                              <  1:924643/93‑1 (MQ=255)
                                           tGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAgg                                              <  2:4257570/93‑1 (MQ=255)
                                           tGCCGCAGCTAACGTACCGGCATGTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAgg                                              <  2:2110424/93‑1 (MQ=255)
                                            gCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAggg                                             <  1:502854/93‑1 (MQ=255)
                                                 aGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGggtggt                                        <  1:2646767/93‑1 (MQ=255)
                                                  gCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGggtgggt                                       >  1:2104697/1‑91 (MQ=255)
                                                  gCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGtt                                       >  1:3739199/1‑93 (MQ=255)
                                                  gCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGtt                                       <  2:2000971/93‑1 (MQ=255)
                                                   cTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGttt                                      <  2:518030/93‑1 (MQ=255)
                                                   cTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGGGGttt                                      >  1:4532868/1‑93 (MQ=255)
                                                    tAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTc                                     >  1:197646/1‑93 (MQ=255)
                                                     aaCGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCa                                    >  1:1326092/1‑93 (MQ=255)
                                                      aCGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCAc                                   >  1:1190039/1‑93 (MQ=255)
                                                        gTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCAccc                                 <  2:2536889/93‑1 (MQ=255)
                                                        gTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCAccc                                 <  2:2405148/93‑1 (MQ=255)
                                                         tACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCAcccc                                <  1:4241610/93‑1 (MQ=255)
                                                         tACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCAcccc                                <  2:359702/93‑1 (MQ=255)
                                                           ccGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCtt                              >  2:3327539/1‑93 (MQ=255)
                                                            cGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCttt                             <  2:2197552/93‑1 (MQ=255)
                                                            cGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCCTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCttt                             <  1:2505505/93‑1 (MQ=255)
                                                             ggCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCtttt                            <  2:4280943/93‑1 (MQ=255)
                                                             ggCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCtttt                            <  1:3402750/93‑1 (MQ=255)
                                                             ggCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCtttt                            >  2:1489663/1‑93 (MQ=255)
                                                             ggCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCtttt                            >  1:1458339/1‑93 (MQ=255)
                                                              gCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTg                           <  1:3284469/93‑1 (MQ=255)
                                                              gCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTg                           >  1:3380097/1‑93 (MQ=255)
                                                                aTTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACATTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTc                         <  2:1877497/93‑1 (MQ=255)
                                                                aTTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGTGGAGGGTTCACCCCTTgtgtt                         >  1:1404698/1‑92 (MQ=255)
                                                                aTTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGtt                         >  2:1405593/1‑92 (MQ=255)
                                                                 tttGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCt                        >  1:4429746/1‑93 (MQ=255)
                                                                   tGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCttt                      <  2:3421400/93‑1 (MQ=255)
                                                                    gTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTc                     <  1:2190805/93‑1 (MQ=255)
                                                                     tttCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCt                    <  2:835201/93‑1 (MQ=255)
                                                                     tttCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCt                    >  1:2721440/1‑93 (MQ=255)
                                                                     tttCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCt                    <  2:1219267/93‑1 (MQ=255)
                                                                        cTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGc                 >  1:3445375/1‑93 (MQ=255)
                                                                        cTGGCAAGACACAGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTCTTAACGAAGGGGTGTTTTCACCCCTTTTGTCTTTCTGGc                 <  2:16076/93‑1 (MQ=255)
                                                                         tGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCg                <  1:1017368/93‑1 (MQ=255)
                                                                         tGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCg                >  2:3960372/1‑93 (MQ=255)
                                                                         tGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCg                <  2:1732827/93‑1 (MQ=255)
                                                                          ggCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGt               <  1:657617/93‑1 (MQ=255)
                                                                             aaGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGa            >  2:1480010/1‑93 (MQ=255)
                                                                              aGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGAt           <  1:684552/93‑1 (MQ=255)
                                                                                gCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCa         <  2:2641504/93‑1 (MQ=255)
                                                                                 cACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCAt        <  1:780663/93‑1 (MQ=255)
                                                                                   cTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTg      <  2:1982281/93‑1 (MQ=255)
                                                                                    tGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGa     >  2:2745032/1‑93 (MQ=255)
                                                                                    tGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGa     <  1:963535/93‑1 (MQ=255)
                                                                                     gAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGAt    <  2:700423/93‑1 (MQ=255)
                                                                                     gAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGAt    <  1:2872688/93‑1 (MQ=255)
                                                                                     gAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGAt    >  2:125162/1‑93 (MQ=255)
                                                                                     gAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGAt    <  2:2449372/93‑1 (MQ=255)
                                                                                      aaaGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATg   >  1:3907228/1‑93 (MQ=255)
                                                                                       aaGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATgc  <  1:4091260/93‑1 (MQ=255)
                                                                                          |                                                                                         
AGCGTACTGGCCGCAACCCGCAGACCGGTAAAGAAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGACGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGC  >  NC_000913/4198028‑4198207

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CTGAGCGTACTGGCCGCAACCCGCAGACCGGTAAAGAAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGACGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCTGG  >  NC_000913/4198025‑4198210
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CTGAGCGTACTGGCCGCAACCCGCAGACCGGTAAAGAAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGG                                                                                              <  SRR4302535.3818482/94‑1 (MQ=60)
   AGCGTACTGGCCGCAACCCGCAGACCGGTAAAGAAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGC                                                                                           <  SRR4302535.1679719/94‑1 (MQ=60)
     CGTACTGGCCGCAACCCGCAGACCGGTAAAGAAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAG                                                                                         >  SRR4302535.3950477/1‑94 (MQ=60)
     CGTACTGGCCGCAACCCGCAGACCGGTAAAGAAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAG                                                                                         <  SRR4302535.3626413/94‑1 (MQ=60)
      GTACTGGCCGCAACCCGCAGACCGGTAAAGAAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGT                                                                                        >  SRR4302535.2638473/1‑94 (MQ=60)
        ACTGGCCGCAACCCGCAGACCGGTAAAGAAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTA                                                                                      >  SRR4302535.1687498/1‑94 (MQ=60)
         CTGGCCGCAACCCGCAGACCGGTAAAGAAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAA                                                                                     <  SRR4302535.4457196/94‑1 (MQ=60)
           GGCCGCAACCCGCAGACCGGTAAAGAAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGA                                                                                   >  SRR4302535.1303290/1‑94 (MQ=60)
            GCCGCAACCCGCAGACCGGTAAAGAAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTA                                                                                  <  SRR4302535.4430281/94‑1 (MQ=60)
             CCGCAACCCGCAGACCGGTAAAGAAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAA                                                                                 <  SRR4302535.569551/94‑1 (MQ=60)
             CCGCAACCCGCAGACCGGTAAAGAAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAA                                                                                 <  SRR4302535.3040332/94‑1 (MQ=60)
              CGCAACCCGCAGACCGGTAAAGAAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAG                                                                                <  SRR4302535.4630089/94‑1 (MQ=60)
                CAACCCGCAGACCGGTAAAGAAATCAAAATTGCCGCAGCTAACTTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGAT                                                                              <  SRR4302535.2706146/94‑1 (MQ=60)
                CAACCCGCAGACCGGTAAAGAAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGAT                                                                              >  SRR4302535.3269020/1‑94 (MQ=60)
                CAACCCGCAGACCGGTAAAGAAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGAT                                                                              <  SRR4302535.3372591/94‑1 (MQ=60)
                CAACCCGCAGACCGGTAAAGAAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGAT                                                                              <  SRR4302535.3384003/94‑1 (MQ=60)
                CAACCCGCAGACCGGTAAAGAAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGAT                                                                              <  SRR4302535.1119483/94‑1 (MQ=60)
                 AACCCGCAGACCGGTAAAGAAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATT                                                                             >  SRR4302535.657800/1‑94 (MQ=60)
                 AACCCGCAGACCGGTAAAGAAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATT                                                                             <  SRR4302535.2200226/94‑1 (MQ=60)
                 AACCAGCAGACCGGTAAAGAAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATT                                                                             <  SRR4302535.683116/94‑1 (MQ=60)
                  ACCCGCAGACCGGTAAAGAAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTG                                                                            <  SRR4302535.1413045/94‑1 (MQ=60)
                  ACCCGCAGACCGGTAAAGAAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTG                                                                            <  SRR4302535.27175/94‑1 (MQ=60)
                    CCGCAGACCGGTAAAGAAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCATGGCACTGAAAGGCGCAGTTAAGTAAGATTGCG                                                                          >  SRR4302535.2008111/1‑94 (MQ=60)
                    CCGCAGACCGGTAAAGAAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCG                                                                          >  SRR4302535.2797010/1‑94 (MQ=60)
                       CAGACCGGTAAAGAAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGG                                                                       >  SRR4302535.964979/1‑94 (MQ=60)
                       CAGACCGGTAAAGAAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGG                                                                       <  SRR4302535.3107672/94‑1 (MQ=60)
                        AGACCGGTAAAGAAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGC                                                                      >  SRR4302535.1773270/1‑94 (MQ=60)
                        AGACCGGTAAAGAAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGC                                                                      >  SRR4302535.4227218/1‑94 (MQ=60)
                        AGACCGGTAAAGAAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGC                                                                      >  SRR4302535.737558/1‑94 (MQ=60)
                            CGGTAAAGAAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTG                                                                  >  SRR4302535.2333927/1‑94 (MQ=60)
                             GGTAAAGAAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGA                                                                 >  SRR4302535.2454687/1‑94 (MQ=60)
                              GTAAAGAAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAA                                                                >  SRR4302535.584974/1‑94 (MQ=60)
                               TAAAGAAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAAC                                                               <  SRR4302535.4553055/94‑1 (MQ=60)
                                AAAGAAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACA                                                              >  SRR4302535.2951034/1‑94 (MQ=60)
                                AAAGAAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACA                                                              <  SRR4302535.2174244/94‑1 (MQ=60)
                                 AAGAAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAG                                                             >  SRR4302535.2957425/1‑94 (MQ=60)
                                 AAGAAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAG                                                             >  SRR4302535.3281322/1‑94 (MQ=60)
                                  AGAAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGT                                                            <  SRR4302535.2975454/94‑1 (MQ=60)
                                  AGAAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGT                                                            <  SRR4302535.563522/94‑1 (MQ=60)
                                   GAAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTT                                                           >  SRR4302535.3419673/1‑94 (MQ=60)
                                   GAAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTT                                                           >  SRR4302535.606102/1‑94 (MQ=60)
                                   GAAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTT                                                           <  SRR4302535.3973598/94‑1 (MQ=60)
                                    TAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTT                                                          >  SRR4302535.3880487/1‑94 (MQ=60)
                                    AAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTT                                                          >  SRR4302535.132314/1‑94 (MQ=60)
                                    AAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTT                                                          >  SRR4302535.2776941/1‑94 (MQ=60)
                                    AAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTT                                                          <  SRR4302535.4570884/94‑1 (MQ=60)
                                     AATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTT                                                         >  SRR4302535.2220890/1‑94 (MQ=60)
                                        CAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAAC                                                      >  SRR4302535.525782/1‑94 (MQ=60)
                                         AAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACG                                                     <  SRR4302535.2344575/94‑1 (MQ=60)
                                         AAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACG                                                     >  SRR4302535.4589897/1‑94 (MQ=60)
                                          AAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGA                                                    <  SRR4302535.3900442/94‑1 (MQ=60)
                                          AAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGA                                                    <  SRR4302535.596237/94‑1 (MQ=60)
                                           AATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAA                                                   <  SRR4302535.3898647/94‑1 (MQ=60)
                                            ATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGGGCAGTTAAGTAAGATTGCGGGCCAGTGAACAGATTTAACGAAG                                                  >  SRR4302535.414351/1‑94 (MQ=60)
                                            ATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAG                                                  <  SRR4302535.3047008/94‑1 (MQ=60)
                                            ATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAG                                                  >  SRR4302535.3078849/1‑94 (MQ=60)
                                            ATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAG                                                  >  SRR4302535.4165011/1‑94 (MQ=60)
                                            ATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAG                                                  <  SRR4302535.4625566/94‑1 (MQ=60)
                                             TTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGG                                                 <  SRR4302535.28801/94‑1 (MQ=60)
                                             TTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGG                                                 <  SRR4302535.868559/94‑1 (MQ=60)
                                             TTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTGAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGG                                                 <  SRR4302535.1803063/94‑1 (MQ=60)
                                              TGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGG                                                <  SRR4302535.931190/94‑1 (MQ=60)
                                              TGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGG                                                <  SRR4302535.2803517/94‑1 (MQ=60)
                                               GCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGG                                               <  SRR4302535.506370/94‑1 (MQ=60)
                                                CCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGT                                              <  SRR4302535.2261715/94‑1 (MQ=60)
                                                CCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGT                                              <  SRR4302535.3179609/94‑1 (MQ=60)
                                                 CGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTG                                             <  SRR4302535.1516118/94‑1 (MQ=60)
                                                   CAGCTAACGTACCGGCGTTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGT                                           <  SRR4302535.806980/94‑1 (MQ=60)
                                                   CAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGT                                           >  SRR4302535.176552/1‑94 (MQ=60)
                                                   CAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGT                                           <  SRR4302535.924851/94‑1 (MQ=60)
                                                   CAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGT                                           <  SRR4302535.4675204/94‑1 (MQ=60)
                                                    AGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTT                                          <  SRR4302535.3074717/94‑1 (MQ=60)
                                                    AGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTT                                          >  SRR4302535.4150064/1‑94 (MQ=60)
                                                    AGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTT                                          >  SRR4302535.3765078/1‑94 (MQ=60)
                                                    AGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTT                                          <  SRR4302535.2665725/94‑1 (MQ=60)
                                                    AGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTT                                          >  SRR4302535.3519328/1‑94 (MQ=60)
                                                    AGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTT                                          >  SRR4302535.4509596/1‑94 (MQ=60)
                                                    AGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGGT                                          >  SRR4302535.2119876/1‑94 (MQ=60)
                                                     GCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTT                                         >  SRR4302535.2719198/1‑94 (MQ=60)
                                                     GCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTT                                         <  SRR4302535.3927016/94‑1 (MQ=60)
                                                     GCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGGGGTTT                                         >  SRR4302535.4563892/1‑94 (MQ=60)
                                                      CTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTCACGAAGGGGTGGTTTC                                        >  SRR4302535.4605656/1‑94 (MQ=60)
                                                      CTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTC                                        >  SRR4302535.199005/1‑94 (MQ=60)
                                                      CTAACGTACCGGCATTTGTTTCTGGCAAAGCACTGAAAGGAGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTC                                        >  SRR4302535.2914673/1‑94 (MQ=60)
                                                       TAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCA                                       >  SRR4302535.1335557/1‑94 (MQ=60)
                                                        AACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCAC                                      >  SRR4302535.894626/1‑94 (MQ=60)
                                                        AACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCAC                                      >  SRR4302535.1198559/1‑94 (MQ=60)
                                                         ACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACC                                     >  SRR4302535.343005/1‑94 (MQ=60)
                                                           GTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCC                                   >  SRR4302535.820702/1‑94 (MQ=60)
                                                           GTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCC                                   >  SRR4302535.2969933/1‑94 (MQ=60)
                                                            TACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCT                                  <  SRR4302535.4270641/94‑1 (MQ=60)
                                                            TACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCT                                  >  SRR4302535.1159432/1‑94 (MQ=60)
                                                              CCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTT                                <  SRR4302535.1133559/94‑1 (MQ=60)
                                                               CGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTT                               >  SRR4302535.1468777/1‑94 (MQ=60)
                                                               CGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCCTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTT                               <  SRR4302535.2523566/94‑1 (MQ=60)
                                                                GGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTG                              <  SRR4302535.3426429/94‑1 (MQ=60)
                                                                GGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTG                              >  SRR4302535.3403598/1‑94 (MQ=60)
                                                                 GCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGT                             >  SRR4302535.265004/1‑94 (MQ=60)
                                                                 GCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGT                             <  SRR4302535.2701103/94‑1 (MQ=60)
                                                                 GCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGT                             <  SRR4302535.3307300/94‑1 (MQ=60)
                                                                 GCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGT                             >  SRR4302535.2854493/1‑94 (MQ=60)
                                                                  CATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGTGGAGGGTTCACCCCTTgtgtt                            >  SRR4302535.1414742/1‑89 (MQ=60)
                                                                  CATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTC                            >  SRR4302535.1380549/1‑94 (MQ=60)
                                                                   ATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCT                           >  SRR4302535.4460097/1‑94 (MQ=60)
                                                                   ATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCT                           <  SRR4302535.2211044/94‑1 (MQ=60)
                                                                    TTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTCAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCTT                          >  SRR4302535.4594003/1‑94 (MQ=60)
                                                                      TGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTC                        >  SRR4302535.632846/1‑94 (MQ=60)
                                                                      TGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTC                        >  SRR4302535.3067503/1‑94 (MQ=60)
                                                                      TGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTC                        >  SRR4302535.3412648/1‑94 (MQ=60)
                                                                       GTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCT                       <  SRR4302535.2206400/94‑1 (MQ=60)
                                                                       GTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCT                       >  SRR4302535.2740879/1‑94 (MQ=60)
                                                                       GTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCT                       >  SRR4302535.2229689/1‑94 (MQ=60)
                                                                       GTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCT                       >  SRR4302535.2565617/1‑94 (MQ=60)
                                                                          TCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGC                    >  SRR4302535.3469331/1‑94 (MQ=60)
                                                                           CTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCG                   >  SRR4302535.1035349/1‑94 (MQ=60)
                                                                           CTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCG                   >  SRR4302535.181251/1‑94 (MQ=60)
                                                                            TGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGT                  <  SRR4302535.1024599/94‑1 (MQ=60)
                                                                             GGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTC                 <  SRR4302535.987841/94‑1 (MQ=60)
                                                                             GGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTC                 <  SRR4302535.662207/94‑1 (MQ=60)
                                                                                AAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGAT              <  SRR4302535.1303745/94‑1 (MQ=60)
                                                                                 AGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATC             <  SRR4302535.689345/94‑1 (MQ=60)
                                                                                  GGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCA            <  SRR4302535.1299544/94‑1 (MQ=60)
                                                                                    CACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATT          <  SRR4302535.4652060/94‑1 (MQ=60)
                                                                                    CACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATT          <  SRR4302535.786142/94‑1 (MQ=60)
                                                                                    CACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATT          >  SRR4302535.2639738/1‑94 (MQ=60)
                                                                                     ACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTG         <  SRR4302535.2045001/94‑1 (MQ=60)
                                                                                       TGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGAT       >  SRR4302535.683960/1‑94 (MQ=60)
                                                                                       TGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGAT       <  SRR4302535.1449177/94‑1 (MQ=60)
                                                                                       TGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGAT       <  SRR4302535.970380/94‑1 (MQ=60)
                                                                                       TGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGAT       >  SRR4302535.517006/1‑94 (MQ=60)
                                                                                        GAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATG      <  SRR4302535.2893044/94‑1 (MQ=60)
                                                                                        GAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATG      >  SRR4302535.3933997/1‑94 (MQ=60)
                                                                                          AAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCT    >  SRR4302535.2343321/1‑94 (MQ=60)
                                                                                          AAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCT    <  SRR4302535.4119254/94‑1 (MQ=60)
                                                                                           AGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCTG   >  SRR4302535.1839327/1‑94 (MQ=60)
                                                                                            GGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCTGG  <  SRR4302535.2416756/94‑1 (MQ=60)
                                                                                             |                                                                                            
CTGAGCGTACTGGCCGCAACCCGCAGACCGGTAAAGAAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGACGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCTGG  >  NC_000913/4198025‑4198210

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑