Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1873 183.1 9376148 98.2% 9207377 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,241,286 T→C T457A (ACG→GCG)  malF ← MalF

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,241,2860TC99.5% 633.6 / ‑5.4 208T457A (ACG→GCG) malFMalF
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base C (81/126);  total (81/127)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.45e-01

Only 100 of 238 total aligned reads displayed.

CCAGACCGAAGTCCTGACCCCCGCCGCCTTCAAAAGCGATGCGGTAGGTGTAGTTAACAAGCAGGTCGGTATAACCGGCTGGCGTGGTCGTGCCAAGACGATCCGGGCCGCCGTTGGTTAACAGTTGAATCAGCACGAAGTTGTTAAAGTTAAAGGCGAAGCTGGCGATCATCAGCGGC  >  NC_000913/4241196‑4241374
                                                                                          |                                                                                        
ccAGACCGAAGTCCTGACCCCCGCCGCCTTCAAAAGCGATGCGGTAGGTGTAGTTCACAAGCAGGTCGGTATAACCGGCTGGCGTGGTCgcgc                                                                                        >  1:3039473/1‑93 (MQ=255)
  aGACCGAAGTCCTGACCCCCGCCGCCTTCAAAAGCGATGCGGTAGGTGTAGTTAACAAGCAGGTCGGTATAACCGGCTGGCGTGGTCGCGCCa                                                                                      >  2:2812200/1‑93 (MQ=255)
   gACCGAAGTCCTGACCCCCGCCGCCTTCAAAAGCGATGCGGTAGGTGTAGTTAACAAGCAGGTCGGTATAACCGGCTGGCGTGGTCGCGCCaa                                                                                     >  1:3928420/1‑93 (MQ=255)
   gACCGAAGTCCTGACCCCCGCCGCCTTCAAAAGCGATGCGGTAGGTGTAGTTAACAAGCAGGACGGAATAACAGGCTGGCGTGGTCGCGCCaa                                                                                     >  1:1012213/1‑93 (MQ=255)
     ccGAAGTCCTGACCCCCGCCGCCTTCAAAAGCGATGCGGTAGGTGTAGTTAACAAGCAGGTCGGTATAACCGGCTGGCGTGGTCGCGCCAAGa                                                                                   <  2:4067783/93‑1 (MQ=255)
     ccGAAGTCCTGACCCCCGCCGCCTTCAAAAGCGATGCGGTAGGTGTAGTTAACAAGCAGGTCGGTATAACCGGCTGGCGTGGTCGCGCCAAGa                                                                                   <  1:1853529/93‑1 (MQ=255)
     ccGAAGTCCTGACCCCCGCCGCCTTCAAAAGCGATGCGGTAGGTGTAGTTAACAAGCAGGTCGGTATAACCGGCTGGCGTGGGCGCGCCAAGa                                                                                   >  1:3226665/1‑93 (MQ=255)
      cGAAGTCCTGACCCCCGCCGCCTTCAAAAGCGATGCGGTAGGTGTAGTTAACAAGCAGGTCGGTATAACCGGCTGGCGTGGTCGCGGCAAGag                                                                                  >  2:535796/1‑92 (MQ=255)
      cGAAGTCCTGACCCCCGCCGCCTTCAAAAGCGATGCGGTAGGTGTAGTTAACAAGCAGGTCGGTATAACCGGCTGGCGTGGTCGCGCCAAGAc                                                                                  <  2:3666822/93‑1 (MQ=255)
      cGAAGTCCTGACCCCCGCCGCCTACAAAAGCGATGCGGTAGGTGTAGTTAACAAGCAGGTCGGTATAACCGGCTGGCGTGGTCGCGCCAAGAc                                                                                  <  2:3122426/93‑1 (MQ=255)
         aGTCCTGACCCCCGCCGCCTTCAAAAGCGATGCGGTAGGTGTAGTTAACAAGCAGGTCGGTATAACCGGCTGGCGTGGTCGCGCCAAGACGAt                                                                               <  2:268898/93‑1 (MQ=255)
          gTCCTGACCCCCGCCGCCTTCAAAAGCGATGCGGTAGGTGTAGTTAACAAGCAGGTCGGTATAACCGGCTGGCGTGGTCGCGCCAAGACGATc                                                                              <  2:253499/93‑1 (MQ=255)
          gTCCTGACCCCCGCCGCCTTCAAAAGCGATGCGGTAGGTGTAGTTAACAAGCAGGTCGGTATAACCGGCTGGCGTGGTCGCGCCAAGACGATc                                                                              <  2:2227897/93‑1 (MQ=255)
           tCCTGACCCCCGCCGCCTTCAAAAGCGATGCGGTAGGTGTAGTTAACAAGCAGGTCGGTATAACCGGCTGGCGTGGTCGCGCCAAGACGATcc                                                                             <  1:2638995/93‑1 (MQ=255)
           tCCTGACCCCCGCCGCCTTAAAAAGCGATGCGGTAGGTGTAGTTAACAAGCAGGTCGGTATAACCGGCTGGCGTGGTCGCGCCAAGACGATcc                                                                             <  1:220425/93‑1 (MQ=255)
            ccTGGCCCCCGCCGCCTTCAAAAGCGATGCGGTAGGTGTAGTTAACAAGCAGGTCGGTATAACCGGCTGGCGTGGTCGCGCCAAGACGATCCg                                                                            <  1:2621966/93‑1 (MQ=255)
            ccTGACCCCCGCCGCCTTCAAAAGCGATGCGGTAGGTGTAGTTAACAAGCAGGTCGGTATACCCGGCTGGCGTGGTCGCGCCAAGACGATCCg                                                                            <  1:591492/93‑1 (MQ=255)
            ccTGACCCCCGCCGCCTTCAAAAGCGATGCGGTAGGTGTAGTTAACAAGCAGGTCGGTATAACCGGCTGGCGTGGTCGCGCCAAGACGATCCg                                                                            <  1:1162738/93‑1 (MQ=255)
             cTGCCCCCCGCCGCCTTCAAAAGCGATGCGGTAGGTGTAGTTAACAAGCAGGTCGGTATAACCGGCTGGCGTGGTCGCGCCAAGACGATCCgg                                                                           <  2:4183726/93‑1 (MQ=255)
                aCCCCCGCCGCCTTCAAAAGCGATGCGGTAGGTGTAGTTAACAAGCAGGTCGGTATAACCGGCTGGCGTGGTCGCGCCAAGACGATCCGGgcc                                                                        <  2:2465860/93‑1 (MQ=255)
                  ccccGCCGCCTTCAAAAGCGATGCGGTAGGTGTAGTTAACAAGCAGGTCGGTATAACCGGCTGGCGTGGTCCCGCCGAGACGATCCGTgcagc                                                                      >  2:2209588/1‑93 (MQ=255)
                   cccGCCGCCTTCAAAAGCGATGCGGTAGGTGTAGTTAACAAGCAGGTCGGTATAACCGGCTGGCGTGGTCGCGCCAAGACGATCCGGgccgcc                                                                     <  1:2033020/93‑1 (MQ=255)
                   cccGCCGCCTTCAAAAGCGATGCGGTAGGTGTAGTTAACAAGCAGGTCGGTATAACCGGCTGGCGTGGTCGCGCCAAGACGATCCGGgccgcc                                                                     >  2:591288/1‑93 (MQ=255)
                        cgccTTCAAAAGCGATGCGGTAGGTGTAGTTAACAAGCAGGTCGGTATAACCGGCTGGCGTGGTCGCGCCAAGACGATCCGGGCCGCCGTTgg                                                                >  1:2881572/1‑93 (MQ=255)
                         gccTTCAAAAGCGATGCGGTAGGTGTAGTTAACAAGCAGGTCGGTATAACCGGCTGGAGTGGTCGCGCCAAGACGATCCGCGCCGCCGTTGGt                                                               >  1:798203/1‑93 (MQ=255)
                          ccTTCAAAAGCGATGCGGTAGGTGTAGTTAACAAGCAGGTCGGTATAACCGGCTGGAGTGGTCGCGCCAAGACGATCCGGGACGCCGTTGGtt                                                              >  1:3147102/1‑93 (MQ=255)
                           cTTCAAAAGCGATGCGGTAGGTGTAGTTAACAAGCAGGTCGGTATAACCGGCTGGCGTGGTCGCGCCAAGACGATCCGGGCCGCCGTTGGTTa                                                             >  2:283329/1‑93 (MQ=255)
                            ttCAAAAGCGATGCGGTAGGTGTAGTTAACAAGCAGGTCGGTATAACCGGCTGGCGTGGTCGCGCCAAGACGATCCGGGCCGCCGTTGGTTaa                                                            <  2:4544661/93‑1 (MQ=255)
                            ttCAAAAGCGATGCGGTAGGTGTAGTTAACAAGCAGGTCGGTATAACCGGCTGGCGTGGTCGCGCCAAGACGATCCGGGCCGCCGTTGGTTaa                                                            >  2:1551817/1‑93 (MQ=255)
                             tCAAAAGCGATGCGGTAGGTGTAGTTAACAAGCAGGTCGGTATAACCGGCTGGCGTGGTCGCGCCAAGACGATCCGGGCCGCCGTTGGTTAAc                                                           <  2:1108578/93‑1 (MQ=255)
                             tCAAAAGCGATGCGGTAGGTGTAGTTAACAAGCAGGTCGGTATAACCGGCTGGCGTGGTCGCGCCAAGACGATCCGGGCCGCCGTTGGTTAAc                                                           <  1:276660/93‑1 (MQ=255)
                             tCAAAAGCGATGCGGTAGGTGTAGTTAACAAGCAGGTCGGTATAACCGGCTGGCGTGGTCGCGCCAAGACGATCCGGGCCGCCGTTGGTTAAc                                                           <  2:1320277/93‑1 (MQ=255)
                              cAAAAGCGATGCGGTAGGTGTAGTTAACAAGCAGGTCGGTATAACCGGCTGGCGTGGTCGCGCCAAGACGATCCGGGCCGCCGTTGGTTAACa                                                          <  2:1152430/93‑1 (MQ=255)
                                aaaGCGATGCGGTAGGTGTAGTTAACAAGCAGGTCGGTATAACCGGCTGGCGTGGTCGCGCCAAGACGATCCGGGCCGCCGTTGGTTAACAGt                                                        <  1:1811385/93‑1 (MQ=255)
                                aaaGCGATGCGGTAGGTGTAGTTAACAAGCAGGTCGGTATAACCGGCTGGCGTGGTCGCGCCAAGACGATCCGGGCCGCCGTTGGTTAACAGt                                                        <  1:809260/93‑1 (MQ=255)
                                aaaGCGATGCGGTAGGTGTAGTTAACAAGCAGGTCGGTATAACCGGCTGGCGTGGTCGCGCCAAGACGATCCGGGCCGCCGTTGGTTAACAGt                                                        <  1:2893187/93‑1 (MQ=255)
                                aaaGCGATGCGGTAGGTGTAGTTAACAAGCAGGTCGGTATAACCGGCTGGCGTGGTCGCGCCAAGACGATCCGGGCCGCCGTTGGTTAACAGt                                                        >  2:618524/1‑93 (MQ=255)
                                aaaGCGATGCGGTAGGTGTAGTTAACAAGCAGGTCGGTATAACCGGCTGGCGTGGTCGCGCCAAGACGATCCGGGCCGCCGTTGGTTAACAGt                                                        >  2:2321157/1‑93 (MQ=255)
                                aaaGCGATGCGGTAGGTGTAGTTAACAAGCAGGTCGGTATAACCGGCTGGCGTGGTCGCGCCAAGACGATCCGGGCCGCCGCTGGGTTACAGt                                                        >  2:2902427/1‑93 (MQ=255)
                                  aGCGATGCGGTAGGTGTAGTTAACAAGCAGGTCGGTATAACCGGCTGGCGTGGTCGCGCCAAGACGATCCGGGCCGCCGTTGGTTAACAGTTg                                                      <  1:3348495/93‑1 (MQ=255)
                                    cGATGCGGTAGGTGTAGTTAACAAGCAGGTCGGTATAACCGGCTGGCGTGGTCGCGCCAAGACGATCCGGGCCGCCGTTGGTTAACAGTTGaa                                                    <  2:1240845/93‑1 (MQ=255)
                                     gATGCGGTAGGTGTAGTTAACAAGCAGGTCGGTATAACCGGCTGGCGTGGTCGCGCCAAGACGATCCGGGCCGCCGTTGGTTAACAGTTGAAt                                                   <  1:3300516/93‑1 (MQ=255)
                                     gATGCGGTAGGTGTAGTTAACAAGCAGGTCGGTATAACCGGCTGGCGTGGTCGCGCCAAGACGATCCGGGCCGCCGTTGGTTAACAGTTGAAt                                                   >  1:2413822/1‑93 (MQ=255)
                                      aTGCGGTAGGTGTAGTTAACAAGCAGGTCGGTATAACCGGCTGGCGTGGTCGCGCCAAGACGATCCGGGCCGCCGTTGGTTAACAGTTGAATc                                                  <  1:4183883/93‑1 (MQ=255)
                                          ggTAGGTGTAGTTAACAAGCAGGTCGGTATAACCGGCTGGCGTGGTCGCGCCAAGACGATCCGGGCCGCCGTTGGTTAACAGTTGAATCAGCa                                              <  2:860210/93‑1 (MQ=255)
                                            tAGGTGTAGTTAACAAGCAGGTCGGTATAACCGGCTGGCGTGGTCGCGCCAAGACGATCCGGGCCGCCGTTGGTTAACAGTTGAATCAGCACg                                            >  2:2635763/1‑93 (MQ=255)
                                             aGGTGTGGTTAACAAGCAGGTCGGTATAACCGGCTGGCGTGGTCGCGCCAAGACGATCCGGGCCGCCGTTGGTTAACAGTTGAATCAGCACGa                                           <  1:1281225/93‑1 (MQ=255)
                                              ggTGTAGTTAACAAGCAGGTCGGTATAACCGGCTGGCGTGGTCGCGCCAAGACGATCCGGGCCGCCGTTGGTTAACAGTTGAATCAGCACGaa                                          <  2:3472525/93‑1 (MQ=255)
                                              ggTGTAGTTAACAAGCAGGTCGGTATAACCGGCTGGCGTGGTCGCGCCAAGACGATCCGGGCCGCCGTTGGTTAACAGTTGAATCAGCACGaa                                          <  2:3578456/93‑1 (MQ=255)
                                              ggTGTAGTTAACAAGCAGGTCGGTATAACCGGCTGGCGTGGTCGCGCCAAGACGATCCGGGCCGCCGTTGGTTAACAGTTGAATCAGCACGaa                                          <  1:1580630/93‑1 (MQ=255)
                                               gtgtAGTTAACAAGCAGGTCGGTATAACCCGCTGGCGTGGTCGCGCCAAGACGATCCGGGCCGCCGTTGGTTAACAGTTGAATCAGCACGaat                                         >  2:765018/1‑92 (MQ=255)
                                                 gtAGTTAACAAGCAGGTCGGTATAACCGGCTGGCGTGGTCGCGCCAAGACGATCCGGGCCGCCGTTGGTTAACAGTTGAATCAGCACGAAggt                                       >  1:1648886/1‑91 (MQ=255)
                                                  tAGTTAACAAGCAGGTCGGTATAACCGGCTGGCGTGGTCGCGCCAAGACGATCCGGGCCGCCGTTGGTTAACAGTTGAATCAGCACGAAgttg                                      >  1:4141118/1‑93 (MQ=255)
                                                  tAGTTAACAAGCAGGTCGGTATAACCGGCTGGCGTGGTCGCGCCAAGACGATCCGGGCCGCCGTTGGTTAACAGTTGAATCAGCACGAAgttg                                      >  1:951426/1‑93 (MQ=255)
                                                  tAGTTAACAAGCAGGTCGGTATAACCGGCTGGCGTGGTCGCGCCAAGACGATCCGGGCCGCCGTTGGTTAACAGTTGAATCAGCACGAAgttg                                      <  1:750400/93‑1 (MQ=255)
                                                    gTTAACAAGCAGGTCGGTATAACCGGCTGGCGTGGTCGCGCCAAGACGATCCGGGCCGCCGTTGGTTAACAGTTGAATCAGCACGAAgttgtt                                    <  2:2067885/93‑1 (MQ=255)
                                                     ttAACAAGCAGGTCGGTATAACCGGCTGGCGTGGTCGCGCCAAGACGATCCGGGCCGCCGTTGGTTAATAGTTGAATCAGCACGAAGTTgtta                                   >  1:3438830/1‑93 (MQ=255)
                                                     ttAACAAGCAGGTCGGTATAACCGGCTGGCGTGGTCGCGCCAAGACGATCCGGGCCGCCGTTGGTTAACAGTTGAATCAGCACGAAGTTgtta                                   <  1:4649827/93‑1 (MQ=255)
                                                      tAACAAGCAGGTCGGTATAACCGGCTGGCGTGGTCGCGCCAAGACGATCCGGGCCGCCGTTGGTTAACAGTTGAATCAGCACGAAGTTgttaa                                  <  1:856994/93‑1 (MQ=255)
                                                      tAACAAGCAGGTCGGTATAACCGGCTGGCGTGGTCGCGCCAAGACGATCCGGGCCGCCGTTGGTTAACAGTTGAATCAGCACGAAGTTgttaa                                  >  2:1046597/1‑93 (MQ=255)
                                                       aaCAAGCAGGTCGGTATAACCGGCTGGCGTGGTCGCGCCAAGACGATCCGGGCCGCCGTTGGTTAACAGTTGAATCAGCACGAAGTTgttaaa                                 <  1:3811629/93‑1 (MQ=255)
                                                       aaCAAGCAGGTCGGTATAACCGGCTGGCGTGGTCGCGCCAAGACGATCCGGGCCGCCGTTGGTTAACAGTTGAATCAGCACGAAGTTgttaaa                                 <  1:3861207/93‑1 (MQ=255)
                                                       aaCAAGCAGGTCGGTATAACCGGCTGGCGTGGTCGCGCCAAGACGATCCGGGCCGCCGTTGGTTAACAGTTGAATCAGCACGAAGTTgttaaa                                 <  1:1117429/93‑1 (MQ=255)
                                                       aaCAAGCAGGTCGGTATAACCGGCTGGCGTGGTCGCGCCAAGACGATCCGGGCCGCCGTTGGTTAACAGTTGAATCAGCACGAAGTTgttaaa                                 <  1:4582557/93‑1 (MQ=255)
                                                        aCAAGCAGGTCGGTATAACCGGCTGGCGTGGTCGCGCCAAGACGATCCGGGCCGCCGTTGGTTAACAGTTGAATCAGCACGAAGTTgttaaag                                <  2:3903398/93‑1 (MQ=255)
                                                        aCAAGCAGGTCGGTATAACCGGCTGGCGTGGTCGCGCCAAGACGATCCGGGCCGCCGTTGGTTAACAGTTGAATCAGCACGAAGTTgttaaag                                <  1:1789914/93‑1 (MQ=255)
                                                        aCAAGCAGGTCGGTATAACCGGCTGGCCTGGTTGCGCCAAGCCGGTTCGGGGCGCCGTTGGTTAACAGTTGAATCAGCACGAAGTTgttaaag                                <  1:3115037/93‑1 (MQ=255)
                                                         cAAGCAGGTCGGTATAACCGGCTGGCGTGGTCGCGCCAAGACGATCCGGGCCGCCGTTGGTTAACAGTTGAATCAGCACGAAGTTgttaaagt                               >  1:1327092/1‑93 (MQ=255)
                                                         cAAGCAGGTCGGTATAACCGGCTGGCGTGGTCGCGCCAAGACGATCCGGGCCGCCGTTGGTTAACAGTTGAATCAGCACGAAGTTgttaaagt                               <  2:4388933/93‑1 (MQ=255)
                                                           aGCAGGTCGGTATAACCGGCTGGCGTGGTCGCGCCAAGACGATCCGGGCCGCCGTTGGTTAACAGTTGAATCAGCACGAAGTTgttaaagtta                             >  1:2121096/1‑93 (MQ=255)
                                                            gCAGGTCGGTATAACCGGCTGGCGTGGTCGCGCCAAGACGATCCGGGCCGCCGTTGGTTAACAGGTGAATCAGCACGAAGGTgttaaagttaa                            >  2:1908777/1‑93 (MQ=255)
                                                              aGGTCGGTATAACCGGCTGGCGTGGTCGCGCCAAGACGATCCGGGCCGCCGTTGGTTAACAGTTGAATCAGCACGAAGTTGttaaagttaaag                          >  1:939670/1‑93 (MQ=255)
                                                              aGGTCGGTATAACCGGCTGGCGTGGTCGCGCCAAGACGATCCGGGCCGCCGTTGGTTAACAGTTGAATCAGCACGAAGTTGttaaagttaaag                          >  1:1716009/1‑93 (MQ=255)
                                                              aGGTCGATATAACCGGCTGGCGTGGTCGCGCCAAGACGATCCGGGCCGCCGTTGGTTAACAGTTGAATCAGAACGAAGTTGttaaagttaaag                          <  2:3806235/93‑1 (MQ=255)
                                                               ggTCGGTATAACCGGCTGGCGTGGTCGCGCCAAGACGATCCGGGCCGCCGTTGGTTAACAGTTGAATCAGCACGAAGTTGTTAAAGTTAAAgg                         >  2:3288121/1‑93 (MQ=255)
                                                                gTCGGTATAACCGGCTGGCGTGGTCGCGCCAAGACGATCCGGGCCGCCGTTGGTTAACAGTTGAATCAGCACGAAGTTGTTAAAGTTAAAGGc                        <  1:519249/93‑1 (MQ=255)
                                                                  tggTATAGCCGGCTGGCGTGGTCGCGCCAAGACGATCCGGGCCGCCGTTGGTTAACAGTTGAATCAGCACGAAGTTGTTAAAGTTAAAGGCGa                      <  2:4233319/92‑1 (MQ=255)
                                                                  cGGTATAACCGGCTGGCGTGGTCGCGCCAAGACGATCCGGGCCGCCGTTGGTTAACAGTTGAATCAGCACGAAGTTGTTAAAGTTAAAGGCGa                      <  1:1837800/93‑1 (MQ=255)
                                                                  cGGTATAACCGGCTGGCGTGGTCGCGCCAAGACGATCCGGGCCGCCGTTGGTTAACAGTTGAATCAGCACGAAGTTGTTAAAGTTAAAGGCGa                      <  1:3589223/93‑1 (MQ=255)
                                                                   ggTATAACCGGCTGGCGTGTGCGCGCCAAGACGATCCGGGCCGCCGTTGGTTAACAGTTGAATCAGCACGAAGTTGTTAAAGTTAAAGGCGaa                     <  2:1368601/93‑1 (MQ=255)
                                                                   ggTATAACCGGCTGGCGTGGTCGCGCCAAGACGATCCGGGCCGCCGTTGGTTAACAGTTGAATCAGCACGAAGTTGTTAAAGTTAAAGGCGaa                     <  1:1392746/93‑1 (MQ=255)
                                                                   ggTATAACCGGCTGGCGTGGTCGCGCCAAGACGATCCGGGCCGCCGTTGGTTAACAGTTGAATCAGCACGAAGTTGTTAAAGTTAAAGGCGaa                     <  2:1203584/93‑1 (MQ=255)
                                                                      ataACCGGCTGGCGTGGTCGCGCCAAGACGATCCGGGCCGCCGTTGGTTAACAGTTGAATCAGCACGAAGTTGTTAAAGTTAAAGGCGAAGCt                  <  2:2424187/93‑1 (MQ=255)
                                                                      ataACCGGCTGGCGTGGTCGCGCCAAGACGATCCGGGCCGCCGTTGGTTAACAGTTGAATCAGCACGAAGTTGTTAAAGTTAAAGGCGAAGCt                  <  1:2274191/93‑1 (MQ=255)
                                                                      ataACCGGCTGGCGTGGTCGCGCCAAGACGATCCGGGCCGCCGTTGGTTAACAGTTGAATCAGCACGAAGTTGTTAAAGTTAAAGGCGAAGCt                  >  2:1535068/1‑93 (MQ=255)
                                                                       taACCGGCTGGCGTGGTCGCGCCAAGACGATCCGGGCCGCCGTTGGTTAACAGTTGAATCAGCACGAAGTTGTTAAAGTTAAAGGCGAAGCTg                 >  1:4274975/1‑93 (MQ=255)
                                                                       taACCGGCTGGCGTGGTCGCGCCAAGACGATCCGGGCCGCCGTTGGTTAACAGTTGAATCAGCACGAAGTTGTTAAAGTTAAAGGCGAAGCTg                 <  2:1945561/93‑1 (MQ=255)
                                                                        aaCCGGCTGGCGTGGTCGCGCCAAGACGATCCGGGCCGCCGTTGGTTAACAGTTGAATCAGCACGAAGTTGTTAAAGTTAAAGGCGAAGCTgg                <  1:1064660/93‑1 (MQ=255)
                                                                        aaCCGGCTGGCGTGGTCGCGCCAAGACGATCCGGGCCGCCGTTGGTTAACAGTTGAATCAGCACGAAGTTGTTAAAGTTAAAGGCGAAGCTgg                <  2:3042579/93‑1 (MQ=255)
                                                                        aaCCGGCTGGCGTGGTCGCGCCAAGACGATCCGGGCCGCCGTTGGTTAACAGTTGAATCAGCACGAAGTTGTTAAAGTTAAAGGCGAAGCTgg                >  2:1746375/1‑93 (MQ=255)
                                                                         aCCGGCTGGCGTGGTCGCGCCAAGACGATCCGGGCCGCCGTTGGTTAACAGTTGAATCAGCACGAAGTTGTTAAAGTTAAAGGCGAAGCTGGc               >  2:4341110/1‑93 (MQ=255)
                                                                          ccGGCTGGCGCGGGCGCGCCAAGACGATCCGGGCCGCCGGTGGTTAACAGTTGAATCAGCACGAAGTTGTTAAAGTTAAAAGCGAAGCTGGCg              >  2:1613867/1‑93 (MQ=255)
                                                                            ggCTGGCGTGGTCGCGCCAAGACGATCCGGGCCGCCGTGGGTTAACAGTTGAATCAGCACGAAGTTGTTAAAGTTAAAGGCGAAGCTGGCGat            <  2:1666412/93‑1 (MQ=255)
                                                                                 gCGTGGTCGCGCCAAGACGATCCGGGCCGCCGTTGGTTAACAGTTGAATCAGCACGAAGTTGTTAAAGTTAAAGGCGAAGCTGGCGAtcatca       <  1:1993725/93‑1 (MQ=255)
                                                                                 gCGTGGTCGCGCCAAGACGATCCGGGCCGCCGTTGGTTAACAGTTGAATCAGCACGAAGTTGTTAAAGTTAAAGGCGAAGCTGGCGAtcatca       <  1:2712634/93‑1 (MQ=255)
                                                                                 gCGTGGTCGCGCCAAGACGATCCGGGCCGCCGTTGGTTAACAGTTGAATCAGCACGAAGTTGTTAAAGTTAAAGGCGAAGCTGGCGAtcatca       <  2:1213551/93‑1 (MQ=255)
                                                                                  cGTGGTCGCGCCAAGACGATCCGGGCCGCCGTTGGTTAACAGTTGAATCAGCACGAAGTTGTTAAAGTTAAAGGCGAAGCTGGCGATCATCAg      <  1:3006582/93‑1 (MQ=255)
                                                                                  cGTGGTCGCGCCAAGACGATCCGGGCCGCCGTTGGTTAACAGTTGAATCAGCACGAAGTTGTTAAAGTTAAAGGCGAAGCTGGCGATCATCAg      <  2:46277/93‑1 (MQ=255)
                                                                                   gTGGTCGCGCCAAGACGATCCGGGCCGCCGTTGGTTAACAGTTGAATCAGCACGAAGTTGTTAAAGTTAAAGGCGAAGCTGGCGATCATCAgc     <  1:4071717/93‑1 (MQ=255)
                                                                                      gTCGCGCCAAGACGATCCGGGCCGCCGTTGGTTAACAGTTGAATCAGCACGAAGTTGTTAAAGTTAAAGGCGAAGCTGGCGATCATCAgcggc  <  1:2917264/93‑1 (MQ=255)
                                                                                          |                                                                                        
CCAGACCGAAGTCCTGACCCCCGCCGCCTTCAAAAGCGATGCGGTAGGTGTAGTTAACAAGCAGGTCGGTATAACCGGCTGGCGTGGTCGTGCCAAGACGATCCGGGCCGCCGTTGGTTAACAGTTGAATCAGCACGAAGTTGTTAAAGTTAAAGGCGAAGCTGGCGATCATCAGCGGC  >  NC_000913/4241196‑4241374

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CGCCAGACCGAAGTCCTGACCCCCGCCGCCTTCAAAAGCGATGCGGTAGGTGTAGTTAACAAGCAGGTCGGTATAACCGGCTGGCGTGGTCGTGCCAAGACGATCCGGGCCGCCGTTGGTTAACAGTTGAATCAGCACGAAGTTGTTAAAGTTAAAGGCGAAGCTGGCGATCATCAGCGGCGTCA  >  NC_000913/4241194‑4241378
                                                                                            |                                                                                            
CGCCAGACCGAAGTCCTGACCCCCGCCGCCTTCAAAAGCGATGCGGTAGGTGTAGTTAACAAGCAGGTCGGTATAACCGGCTGGCGTGGTCGCG                                                                                             >  SRR4302535.4220946/1‑94 (MQ=60)
 GCCAGACCGAAGTCCTGACCCCCGCCGCCTTCAAAAGCGATGCGGTAGGTGTAGTTCACAAGCAGGTCGGTATAACCGGCTGGCGTGGTCGCGC                                                                                            >  SRR4302535.3060760/1‑94 (MQ=60)
    AGACCGAAGTCCTGACCCCCGCCGCCTTCAAAAGCGATGCGGTAGGTGTAGTTAACAAGCAGGTCGGTATAACCGGCTGGCGTGGTCGCGCCAA                                                                                         >  SRR4302535.1071532/1‑94 (MQ=60)
    AGACCGAAGTCCTGACCCCCGCCGCCTTCAAAAGCGATGCGGTAGGTGTAGTTAACAAGCAGGTCGGTATAACCGGCTGGCGTGGTCGCGCCAA                                                                                         >  SRR4302535.3955253/1‑94 (MQ=60)
    AGACCGAAGTCCTGACCCCCGCCGCCTTCAAAAGCGATGCGGTAGGTGTAGTTAACAAGCAGGACGGAATAACAGGCTGGCGTGGTCGCGCCAA                                                                                         >  SRR4302535.1019409/1‑94 (MQ=60)
      ACCGAAGTCCTGACCCCCGCCGCCTTCAAAAGCGATGCGGTAGGTGTAGTTAACAAGCAGGTCGGTATAACCGGCTGGCGTGGTCGCGCCAAGA                                                                                       >  SRR4302535.2718667/1‑94 (MQ=60)
      ACCGAAGTCCTGACCCCCGCCGCCTTCAAAAGCGATGCGGTAGGTGTAGTTAACAAGCAGGTCGGTATAACCGGCTGGCGTGGGCGCGCCAAGA                                                                                       >  SRR4302535.3249031/1‑94 (MQ=60)
       CCGAAGTCCTGACCCCCGCCGCCTTCAAAAGCGATGCGGTAGGTGTAGTTAACAAGCAGGTCGGTATAACCGGCTGGCGTGGTCGCGCCAAGAC                                                                                      <  SRR4302535.1866878/94‑1 (MQ=60)
        CGAAGTCCTGACCCCCGCCGCCTTCAAAAGCGATGCGGTAGGTGTAGTTAACAAGCAGGTCGGTATAACCGGCTGGCGTGGTCGCGCCAAGACG                                                                                     >  SRR4302535.3090056/1‑94 (MQ=60)
        CGAAGTCCTGACCCCCGCCGCCTTCAAAAGCGATGCGGTAGGTGTAGTTAACAAGCAGGTCGGTATAACCGGCTGGCGTGGTCGCGCCAAGACG                                                                                     >  SRR4302535.1112455/1‑94 (MQ=60)
        CGAAGTCCTGACCCCCGCCGCCTTCAAAAGCGATGCGGTAGGAGTAGTTAACAAGCAGGGCGGTATAACCGGCTGGGGTGGTTGTGCCAACACG                                                                                     >  SRR4302535.2854844/1‑94 (MQ=60)
          AAGTCCTGACCCCCGCCGCCTTCAAAAGCGATGCGGTAGGTGTAGTTAACAAGCAGGTCGGTATAACCGGCTGGCGTGGTCGCGCCAAGACGAT                                                                                   <  SRR4302535.116646/94‑1 (MQ=60)
           AGTCCTGACCCCCGCCGCCTTCAAAAGCGATGCGGTAGGTGTAGTTAACAAGCAGGTCGGTATAACCGGCTGGCGTGGTCGCGCCAAGACGATC                                                                                  <  SRR4302535.3363191/94‑1 (MQ=60)
            GTCCTGACCCCCGCCGCCTTCAAAAGCGATGCGGTAGGTGTAGTTAACAAGCAGGTCGGTATAACCGGCTGGCGTGGTCGCGCCAAGACGATCC                                                                                 <  SRR4302535.4447235/94‑1 (MQ=60)
             TCCTGACCCCCGCCGCCTTCAAAAGCGATGCGGTAGGTGTAGTTAACAAGCAGGTCGGTATAACCGGCTGGCGTGGTCGCGCCAAGACGATCCG                                                                                <  SRR4302535.2657899/94‑1 (MQ=60)
             TCCTGACCCCCGCCGCCTTAAAAAGCGATGCGGTAGGTGTAGTTAACAAGCAGGTCGGTATAACCGGCTGGCGTGGTCGCGCCAAGACGATCCG                                                                                <  SRR4302535.221945/94‑1 (MQ=60)
              CCTGGCCCCCGCCGCCTTCAAAAGCGATGCGGTAGGTGTAGTTAACAAGCAGGTCGGTATAACCGGCTGGCGTGGTCGCGCCAAGACGATCCGG                                                                               <  SRR4302535.2640762/94‑1 (MQ=60)
              CCTGACCCCCGCCGCCTTCAAAAGCGATGCGGTAGGTGTAGTTAACAAGCAGGTCGGTATACCCGGCTGGCGTGGTCGCGCCAAGACGATCCGG                                                                               <  SRR4302535.595636/94‑1 (MQ=60)
              CCTGACCCCCGCCGCCTTCAAAAGCGATGCGGTAGGTGTAGTTAACAAGCAGGTCGGTATAACCGGCTGGCGTGGTCGCGCCAAGACGATCCGG                                                                               <  SRR4302535.1171047/94‑1 (MQ=60)
              CCTGACCCCCGCCGCCTTCAAAAGCGATGCGGTAGGTGTAGTTAACAAGCAGGTCGGTATAACCGGCTGGCGTGGTCGCGCCAAGACGATCCGG                                                                               <  SRR4302535.1721010/94‑1 (MQ=60)
               CTGACCCCCGCCGCCTTCAAAAGCGATGCGGTAGGTGTAGTTAACAAGCAGGTCGGTATAACCGGCTGGCGTGGTCGCGCCAAGACGATCCGGG                                                                              >  SRR4302535.470930/1‑94 (MQ=60)
                TGACCCCCGCCGCCTTCAAAAGCGATGCGGTAGGTGTAGTTAACAAGCAGGTCGGTATAACCGGCTGGCGTGGTCGCGCCAAGACGATCCCGGC                                                                             >  SRR4302535.4003590/1‑94 (MQ=60)
                 GACCCCCGCCGCCTTCAAAAGCGATGCGGTAGGTGTAGTTAACAAGCAGGTCGGTATAACCGGCTGGCGTGGTCGCGCCAAGACGATCCGGGCC                                                                            >  SRR4302535.329060/1‑94 (MQ=60)
                     CCCGCCGCCTTCAAAAGCGATGCGGTAGGTGTAGTTAACAAGCAGGTCGGTATAACCGGCTGGCGTGGTCGCGCCAAGACGATCCGGGCCGCCG                                                                        <  SRR4302535.2047671/94‑1 (MQ=60)
                        GCCGCCTTCAAAAGCGATGCGGTAGGTGTAGTTAACAAGCAGGTCGGTATAACCGGCTGGCGTGGTCGCGCCAAGCCGATCCGGGCCGCCGTTG                                                                     <  SRR4302535.3021574/94‑1 (MQ=60)
                         CCGCCTTCAAAAGCGATGCGGTAGGTGTAGTTAACAAGCAGGTCGGTATAACCGGCTGGCGTGGTCGCGCCAAGACGATCCGGGCCGCCGTTGG                                                                    <  SRR4302535.3054921/94‑1 (MQ=60)
                         CCGCCTTCAAAAGCGATGCGGTAGGTGTAGTTAACAAGCAGGTCGGTATAACCGGCTGGCGTGGTCGCGCCAAGACGATCCGGGCCGCCGTTGG                                                                    >  SRR4302535.2901967/1‑94 (MQ=60)
                         CCGCCTTCAAAAGCGATGCGGTAGGTGTAGTTAACAAGCAGGTCGGTATAACCGGCTGGCGTGGCCGCGCCAAGACGATCCGGGCCGCCGTTGG                                                                    >  SRR4302535.1308925/1‑94 (MQ=60)
                          CGCCTTCAAAAGCGATGCGGTAGGTGTAGTTAACAAGCAGGTCGGTATAACCGGCTGGCGTGGTCGCGCCAAGACGATCCGGGCCGCCGTTGGT                                                                   <  SRR4302535.2202034/94‑1 (MQ=60)
                          CGCCTTCAAAAGCGATGCGGTAGGTGTAGTTAACAAGCAGGTCGGTATAACCGGCTGGCGTGGTCGCGCCAAGACGATCCGGGCCGCCGTTGGT                                                                   <  SRR4302535.791031/94‑1 (MQ=60)
                          CGCCTTCAAAAGCGATGCGGTAGGTGTAGTTAACAAGCAGGTCGGTATAACCGGCTGGAGTGGTCGCGCCAAGACGATCCGCGCCGCCGTTGGT                                                                   >  SRR4302535.803807/1‑94 (MQ=60)
                           GCCTTCAAAAGCGATGCGGTAGGTGTAGTTAACAAGCAGGTCGGTATAACCGGCTGGAGTGGTCGCGCCAAGACGATCCGGGACGCCGTTGGTT                                                                  >  SRR4302535.3168983/1‑94 (MQ=60)
                             CTTCAAAAGCGATGCGGTAGGTGTAGTTAACAAGCAGGTCGGTATAACCGGCTGGCGTGGTCGCGCCAAGGAGATCCGGCCCGCCGTTGGTTAA                                                                >  SRR4302535.3868431/1‑94 (MQ=60)
                             CTTCAAAAGCGATGCGGTAGGTGTAGTTAACAAGCAGGTCGGTATAACCGGCTGGCGTGGTCGCGCCAAGACGATCCGGGCCGCCGTTGGTTAA                                                                >  SRR4302535.503185/1‑94 (MQ=60)
                               TCAAAAGCGATGCGGTAGGTGTAGTTAACAAGCAGGTCGGTATAACCGGCTGGCGTGGTCGCGCCAAGACGATCCGGGCCGCCGTTGGTTAACA                                                              <  SRR4302535.1756668/94‑1 (MQ=60)
                               TCAAAAGCGATGCGGTAGGTGTAGTTAACAAGCAGGTCGGTATAACCGGCTGGCGTGGTCGCGCCAAGACGATCCGGGCCGCCGTTGGTTAACA                                                              <  SRR4302535.278592/94‑1 (MQ=60)
                                CAAAAGCGATGCGGTAGGTGTAGTTAACAAGCAGGTCGGTATAACCGGCTGGCGTGGTCGCGCCAAGACGATCCGGGCCGCCGTTGGTTAACAG                                                             <  SRR4302535.4214943/94‑1 (MQ=60)
                                CAAAAGCGATGCGGTAGGTGTAGTTAACAAGCAGGTCGGTATAACCGGCTGGCGTGGTCGCGCCAAGACGATCCGGGCCGCCGTTGGTTAACAG                                                             <  SRR4302535.2688209/94‑1 (MQ=60)
                                 AACAGCGATGCGGTAGGTGTAGTTAACAAGCAGGTCGGTATCACCGACAGCCGTGGTCGCGCCAAGACGATCCGGGCCGCCGTTGGTTAACAGT                                                            <  SRR4302535.3660492/94‑1 (MQ=60)
                                  AAAGCGATGCGGTAGGTGTAGTTAACAAGCAGGTCGGTATAACCGGCTGGCGTGGTCGCGCCAAGACGATCCGGGCCGCCGTTGGTTAACAGTT                                                           <  SRR4302535.814922/94‑1 (MQ=60)
                                  AAAGCGATGCGGTAGGTGTAGTTAACAAGCAGGTCGGTATAACCGGCTGGCGTGGTCGCGCCAAGACGATCCGGGCCGCCGTTGGTTAACAGTT                                                           <  SRR4302535.1824483/94‑1 (MQ=60)
                                  AAAGCGATGCGGTAGGTGTAGTTAACAAGCAGGTCGGTATAACCGGCTGGCGTGGTCGCGCCAAGACGATCCGGGCCGCCGTTGGTTAACAGTT                                                           <  SRR4302535.2913651/94‑1 (MQ=60)
                                  AAAGCGATGCGGTAGGTGTAGTTAACAAGCAGGTCGGTATAACCGGCTGGCGTGGTCGCGCCAAGACGATCCGGGCCGCCGTTGGTTAACAGTT                                                           >  SRR4302535.2643646/1‑94 (MQ=60)
                                   AAGCGATGCGGTAGGTGTAGTTAGCAAGCAGGTCGGTATAACCGGCTGGCGTGGTCGCGCCAAGACGATCCGGGCCGCCGTTGGTTAACAGTTG                                                          <  SRR4302535.2094901/94‑1 (MQ=60)
                                   AAGCGATGCGGTAGGTGTAGTTAACAAGCAGGTCGGTATAACCGGCTGGCGTGGTCGCGCCAAGACGATCCGGGCCGCCGTTGGTTAACAGTTG                                                          <  SRR4302535.914596/94‑1 (MQ=60)
                                    AGCGATGCGGTAGGTGTAGTTAACAAGCAGGTCGGTATAACCGGCTGGCGTGGTCGCGCCAAGACGATCCGGGCCGCCGTTGGTTAACAGTTGA                                                         <  SRR4302535.3371822/94‑1 (MQ=60)
                                      CGATGCGGTAGGTGTAGTTAACAAGCAGGTCGGTATAACCGGCTGGCGTGGTCGCGCCAAGACGATCCGGGCCGCCGTTGGTTAACAGTTGAAT                                                       <  SRR4302535.1807937/94‑1 (MQ=60)
                                      CGATGCGGTAGGTGTAGTTAACAAGCAGGTCGGTATAACCGGCTGGCGTGGTCGCGCCAAGACGATCCGGGCCGCCGTTGGTTAACAGTTGAAT                                                       <  SRR4302535.2906127/94‑1 (MQ=60)
                                      CGATGCGGTAGGTGTAGTTAACAAGCAGGTCGGTATAACCGGCTGGCGTGGTCGCGCCAAGACGATCCGGGCCGCCGTTGGTTAACAGTTGAAT                                                       >  SRR4302535.2431198/1‑94 (MQ=60)
                                       GATGCGGTAGGTGTAGTTAACAAGCAGGTCGGTATAACCGGCTGGCGTGGTCGCGCCAAGACGATCCGGGCCGCCGTTGGTTAACAGTTGAATC                                                      <  SRR4302535.3323468/94‑1 (MQ=60)
                                       GATGCGGTAGGTGTAGTTAACAAGCAGGTCGGGATAACCGGCTGGCGTGGTCGCGCCAAGACCATCCGGGCCGCCGTTGGGTAACAGTTGAATC                                                      >  SRR4302535.1978325/1‑94 (MQ=60)
                                        ATGCGGTAGGTGTAGTTAACAAGCAGGTCGGTATAACCGGCTGGCGTGGTCGCGCCAAGACGATCCGGGCCGCCGTTGGTTAACAGTTGAATCA                                                     <  SRR4302535.1378447/94‑1 (MQ=60)
                                        ATGCGGTAGGTGTAGTTAACAAGCAGGTCGGTATAACCGGCTGGCGTGGTCGCGCCAAGACGATCCGGGCCGCCGTTGGTTAACAGTTGAATCA                                                     <  SRR4302535.4212511/94‑1 (MQ=60)
                                         TGCGGTAGGTGTAGTTAACAAGCAGGTCGGTATAACCGGCTGGCGTGGTCGCGCCAAGACGATCCGGGCCGCCGTTGGTTAACAGTTGAATCAG                                                    <  SRR4302535.3894131/94‑1 (MQ=60)
                                           CGGTAGGTGTAGTTAACAAGCAGGTCGGTATAACCGGCTGGCGTGGTCGCGCCAAGACGATCCGGGCCGCCGTTGGTTAACAGTTGAATCAGCA                                                  <  SRR4302535.1403706/94‑1 (MQ=60)
                                           CGGTAGGTGTAGTTAACAAGCAGGTCGGTATAACCGGCTGGCGTGGTCGCGCCAAGACGATCCGGGCCGCCGTTGGTTAACAGTTGAATCAGCA                                                  <  SRR4302535.1860210/94‑1 (MQ=60)
                                              TAGGTGTAGTTAACAAGCAGGTCGGTATAACCGGCTGGCGTGGTCGCGCCAAGACGATCCGGGCCGCCGTTGGTTAACAGTTGAATCAGCACGA                                               <  SRR4302535.2481590/94‑1 (MQ=60)
                                               AGGTGTGGTTAACAAGCAGGTCGGTATAACCGGCTGGCGTGGTCGCGCCAAGACGATCCGGGCCGCCGTTGGTTAACAGTTGAATCAGCACGAA                                              <  SRR4302535.1290369/94‑1 (MQ=60)
                                                GGTGTAGTTAACAAGCAGGTCGGTATAACCGGCTGGCGTGGTCGCGCCAAGACGATCCGGGCCGCCGTTGGTTAACAGTTGAATCAGCACGAAG                                             <  SRR4302535.1592040/94‑1 (MQ=60)
                                                GGTGTAGTTAACAAGCAGGTCGGTATAACCGGCTGGCGTGGTCGCGCCAAGACGATCCGGGCCGCCGTTGGTTAACAGTTGAATCAGCACGAAG                                             <  SRR4302535.3095108/94‑1 (MQ=60)
                                                 GTGTAGTTAACAAGCAGGTCGGTATAACCGGCTGGCGTGGTCGCGCCAAGACGATCCGGGCCGCCGTTGGTTAACAGTTGAATCAGCACGAAGT                                            >  SRR4302535.830314/1‑94 (MQ=60)
                                                  TGTAGTTAACAAGCAGGTCGGTATAACCGGCTGGCGTGGTCGCGCCAAGACGATCCGGGCCGCCGTTGGTTAACAGTTGAATCAGCACGAAGGT                                           >  SRR4302535.1660814/1‑94 (MQ=60)
                                                   GTAGTTAACAGGCAGGTCGGTATAACCGGCTGGCGTGGTCGCGCCAAGACGATCCGGGCCGCCGTTGGTTAACAGTTGAATCAGCACGAAGTTG                                          <  SRR4302535.3538265/94‑1 (MQ=60)
                                                   GTAGTTAACAAGCAGGTCGGTATAACCGGCTGGCGTGGTCGCGCCAAGACGATCCGGGCCGCCGTTGGTTAACAGTTGAATCAGCACGAAGTTG                                          >  SRR4302535.4169475/1‑94 (MQ=60)
                                                   GTAGTTAACAAGCAGGTCGGTATAACCGGCTGGCGTGGTCGCGCCAAGACGATCCGGGCCGCCGTTGGTTAACAGTTGAATCAGCACGAAGTTG                                          >  SRR4302535.624578/1‑94 (MQ=60)
                                                   GTAGTTAACAAGCAGGTCGGTATAACCGGCTGGCGTGGTCGCGCCAAGACGATCCGGGCCGCCGTTGGTTAACAGTTGAATCAGCACGAAGTTG                                          <  SRR4302535.757129/94‑1 (MQ=60)
                                                   GTAGTTAACAAGCAGGTCGGTATAACCGGCTGGCGTGGTCGCGCCAAGACGATCCGGGCCGCCGTTGGTTAACAGTTGAATCAGCACGAAGTTG                                          >  SRR4302535.3502677/1‑94 (MQ=60)
                                                   GTAGTTAACAAGCAGGTCGGTATAACCGGCTGGCGTGGTCGCGCCAAGACGATCCGGGCCGCCGTTGGTTAACAGTTGAATCAGCACGAAGTTG                                          >  SRR4302535.958164/1‑94 (MQ=60)
                                                    TAGTTAACAAGCAGGTCGGTATAACCGGCTGGCGTGGTCGCGCCAAGACGATCCGGGCCGCCGTTGGTTAACAGTTGAATCAGCACGAAGTTGT                                         <  SRR4302535.755690/94‑1 (MQ=60)
                                                      GTTAACAAGCAGGTCGGTATAACCGGCTGGCGTGGTCGCGCCAAGACGATCCGGGCCGCCGTTGGTTAATAGTTGAATCAGCACGAAGTTGTTA                                       >  SRR4302535.3462748/1‑94 (MQ=60)
                                                      GTTAACAAGCAGGTCGGTATAACCGGCTGGCGTGGTCGCGCCAAGACGATCCGGGCCGCCGTTGGTTAACAGTTGAATCAGCACGAAGTTGTTA                                       >  SRR4302535.3023677/1‑94 (MQ=60)
                                                      GTTAACAAGCAGGTCGGTATAACCGGCTGGCGTGGTCGCGCCAAGACGATCCGGGCCGCCGTTGGTTAACAGTTGAATCAGCACGAAGTTGTTA                                       >  SRR4302535.3866884/1‑94 (MQ=60)
                                                       TTAACAAGCAGGTCGGTATAACCGGCTGGCGTGGTCGCGCCAAGACGATCCGGGCCGCCGTTGGTTAACAGTTGAATCAGCACGAAGTTGTTAA                                      <  SRR4302535.4681319/94‑1 (MQ=60)
                                                       TTAACAAGCAGGTCGGTATAACCGGCTGGCGTGGTCGCGCCAAGACGATCCGGGCCGCCGTTGGTTAACAGTTGAATCAGCACGAAGTTGTTAA                                      <  SRR4302535.1619653/94‑1 (MQ=60)
                                                        TAACAAGCAGGTCGGTATAACCGGCTGGCGTGGTCGCGCCAAGACGATCCGGGCCGCCGTTGGTTAACAGTTGAATCAGCACGAAGTTGTTAAA                                     <  SRR4302535.1132203/94‑1 (MQ=60)
                                                        TAACAAGCAGGTCGGTATAACCGGCTGGCGTGGTCGCGCCAAGACGATCCGGGCCGCCGTTGGTTAACAGTTGAATCAGCACGAAGTTGTTAAA                                     <  SRR4302535.863032/94‑1 (MQ=60)
                                                         AACAAGCAGGTCGGTATAACCGGCTGGCGTGGTCGCGCCAAGACGATCCGGGCCGCCGTTGGTTAACAGTTGAATCAGCACGAAGTTGTTAAAG                                    <  SRR4302535.4613766/94‑1 (MQ=60)
                                                         AACAAGCAGGTCGGTATAACCGGCTGGCGTGGTCGCGCCAAGACGATCCGGGCCGCCGTTGGTTAACAGTTGAATCAGCACGAAGTTGTTAAAG                                    <  SRR4302535.3837927/94‑1 (MQ=60)
                                                         AACAAGCAGGTCGGTATAACCGGCTGGCGTGGTCGCGCCAAGACGATCCGGGCCGCCGTTGGTTAACAGTTGAATCAGCACGAAGTTGTTAAAG                                    <  SRR4302535.3887775/94‑1 (MQ=60)
                                                         AACAAGCAGGTCGGTATAACCGGCTGGCGTGGTCGCGCCAAGACGATCCGGGCCGCCGTTGGTTAACAGTTGAATCAGCACGAAGTTGTTAAAG                                    <  SRR4302535.1125398/94‑1 (MQ=60)
                                                         AACAAGCAGGTCGGTATAACCGGCTGGCGTGGTCGCGCCAAGACGATCCGGGCCGCCGTTGGTTAACAGTTGAATCAGCACGAAGTTGTTAAAG                                    >  SRR4302535.3156515/1‑94 (MQ=60)
                                                         AACAAGCAGGTCGGTATAACCGGCTGGCGTGGTCGCGCCAAGACGATCCGGGCCGCCGTTGGTTAACAGTTGAATCAGCACGAAGCTGTTAAAG                                    >  SRR4302535.2315212/1‑94 (MQ=60)
                                                          ACAAGCAGGTCGGTATAACCGGCTGGCGTGGTCGCGCCAAGACGATCCGGGCCGCCGTTGGTTAACAGTTGAATCAGCACGAAGTTGTTAAAGT                                   <  SRR4302535.1802862/94‑1 (MQ=60)
                                                          ACAAGCAGGTCGGTATAACCGGCTGGCGTGGTCGCGCCAAGACGATCCGGGCCGCCGTTGGTTAACAGTTGAATCAGCACGAAGTTGTTAAAGT                                   >  SRR4302535.1336562/1‑94 (MQ=60)
                                                          ACAAGCAGGTCGGTATAACCGGCTGGCGTGGTCGCGCCAAGACGATCCGGGCCGCCGTTGGTTAACAGTTGAATCAGCACGAAGTTGTTAAAGT                                   >  SRR4302535.2919792/1‑94 (MQ=60)
                                                          ACAAGCAGGTCGGTATAACCGGCTGGCGTGGTCGCGCCAAGACGATCCGGGCCGCCGTTGGTTAACAGTTGAATCAGCACGAAGTTGTTAAAGT                                   >  SRR4302535.1804256/1‑94 (MQ=60)
                                                          ACAAGCAGGTCGGTATAACCGGCTGGCCTGGTTGCGCCAAGCCGGTTCGGGGCGCCGTTGGTTAACAGTTGAATCAGCACGAAGTTGTTAAAGT                                   <  SRR4302535.3136725/94‑1 (MQ=60)
                                                            AAGCAGGTCGGTATAACCGGCTGGCGTGGTCGCGCCAAGACGATCCGGGGCGCCGTTGGTTAACAGTTGAATCAGCACGAAGTTGTTAAAGTTA                                 >  SRR4302535.1902157/1‑94 (MQ=60)
                                                            AAGCAGGTCGGTATAACCGGCTGGCGTGGTCGCGCCAAGACGATCCGGGCCGCCGTTGGTTAACAGTTGAATCAGCACGAAGTTGTTAAAGTTA                                 >  SRR4302535.2136391/1‑94 (MQ=60)
                                                             AGCAGGTCGGTATAACCGGCTGGCGTGGTCGCGCCAAGACGATCCGGGCCGCCGTTGGTTAACAGTTGAATCAGCACGAAGTTGTTAAAGTTAA                                <  SRR4302535.4169620/94‑1 (MQ=60)
                                                               CAGGTCGGTATAACCGGCTGGCGTGGTCGCGCCAAGACGATCCGGGCCGCCGTTGGTTAACAGTTGAATCAGCACGAAGTTGTTAAAGTTAAAG                              >  SRR4302535.946326/1‑94 (MQ=60)
                                                               CAGGTCGGTATAACCGGCTGGCGTGGTCGCGCCAAGACGATCCGGGCCGCCGTTGGTTAACAGTTGAATCAGCACGAAGTTGTTAAAGTTAAAG                              >  SRR4302535.1728422/1‑94 (MQ=60)
                                                                AGGTCGGTATAACCGGCTGGCGTGGTCGCGCCAAGACGATCCGGGCCGCCGTTGGTTAACAGTTGAATCAGCACGAAGTTGTTAAAGTTAAAGG                             <  SRR4302535.852461/94‑1 (MQ=60)
                                                                 GGTCGGTATAACCGGCTGGCGTGGTCGCGCCAAGACGATCCGGGCCGCCGTTGGTTAACAGTTGAATCAGCACGAAGTTGTTAAAGTTAAAGGC                            <  SRR4302535.2422678/94‑1 (MQ=60)
                                                                  GTCGGTATAACCGGCTGGCGTGGTCGCGCCAAGACGATCCGGGCCGCCGTTGGTTAACAGTTGAATCAGCACGAAGTTGTTAAAGTTAAAGGCG                           <  SRR4302535.522883/94‑1 (MQ=60)
                                                                  GTCGGTATAACCGGCTGGCGTGGTCGCGCCAAGACGATCCGGGCCGCCGTTGGTTAACAGTTGAATCAGCACGAAGTTGTTAAAGTTAAAGGCG                           <  SRR4302535.4353962/94‑1 (MQ=60)
                                                                    CGGTATAACCGGCTGGCGTGGTCGCGCCAAGACGATCCGGGCCGCCGTTGGTTAACAGTTGAATCAGCACGAAGTTGTTAAAGTTAAAGGCGAA                         <  SRR4302535.3614121/94‑1 (MQ=60)
                                                                    CGGTATAACCGGCTGGCGTGGTCGCGCCAAGACGATCCGGGCCGCCGTTGGTTAACAGTTGAATCAGCACGAAGTTGTTAAAGTTAAAGGCGAA                         <  SRR4302535.1851059/94‑1 (MQ=60)
                                                                    CGGTATAACCGGCTGGCGTGGTCGCGCCAAGACGATCCGGGCCGCCGTTGGTTAACAGTTGAATCAGCACGAAGTTGTTAAAGTTAAAGGCGAA                         >  SRR4302535.1358718/1‑94 (MQ=60)
                                                                     GGTATAACCGGCTGGCGTGGTCGCGCCAAGACGATCCGGGCCGCCGTTGGTTAACAGTTGACTCAGCACGAAGTTGTTAAAGCTAAAGGCGAAG                        >  SRR4302535.1220062/1‑94 (MQ=60)
                                                                     GGTATAACCGGCTGGCGTGGTCGCGCCAAGACGATCCGGGCCGCCGTTGGTTAACAGTTGAATCAGCACGAAGTTGTTAAAGTTAAAGGCGAAG                        >  SRR4302535.2242842/1‑94 (MQ=60)
                                                                     GGTATAACCGGCTGGCGTGGTCGCGCCAAGACGATCCGGGCCGCCGTTGGTTAACAGTTGAATCAGCACGAAGTTGTTAAAGTTAAAGGCGAAG                        <  SRR4302535.1402690/94‑1 (MQ=60)
                                                                      GTATAACCGGCTGGCGTGGTCGCGCCAAGACGATCCGGGCCGCCGTTGGTTAACAGTTGAATCAGCACGAAGTTGTTAAAGTTAAAGGCGAAGC                       >  SRR4302535.2407722/1‑94 (MQ=60)
                                                                       TATAGCCGGCTGGCGTGGTCGCGCCAAGACGATCCGGGCCGCCGTTGGTTAGCAGTTGAATCAGCACGAAGTTGTTAAAGTTAAAGGCGAAGCT                      <  SRR4302535.331880/94‑1 (MQ=60)
                                                                       TATAACCGGCTGGCGTGGTCGCGCCAAGACGATCCGGGCCGCCGTTGGTTAACAGTTGAATCAGCACGAAGTTGTTAAAGTTAAAGGCGAAGCT                      <  SRR4302535.3196671/94‑1 (MQ=60)
                                                                       GATAACCGGCTGGCGTGGTCGCGCCAAGACGATCCGGGCCGCCGTTGGTTAACAGTTGAATCAGCACGAAGTTGTTAAAGTTAAAGGCGAAGCT                      <  SRR4302535.956011/94‑1 (MQ=60)
                                                                        ATAACCGGCTGGCGTGGTCGCGCCAAGACGATCCGGGCCGCCGTTGGTTAACAGTTGAATCAGCACGAAGTTGTTAAAGTTAAAGGCGAAGCTG                     <  SRR4302535.2290421/94‑1 (MQ=60)
                                                                        ATAACCGGCTGGCGTGGTCGCGCCAAGACGATCCGGGCCGCCGTTGGTTAACAGTTGAATCAGCACGAAGTTGTTAAAGTTAAAGGCGAAGCTG                     <  SRR4302535.560492/94‑1 (MQ=60)
                                                                        ATAACCGGCTGGCGTGGTCGCGCCAAGACGATCCGGGCCGCCGTTGGTTAACAGTTGAATCAGCACGAAGTTGTTAAAGTTAAAGGCGAAGCTG                     <  SRR4302535.3647776/94‑1 (MQ=60)
                                                                        ATAACCGGCTGGCGTGGTCGCGCCAAGACGATCCGGGCCGCCGTTGGTTAACAGTTGAATCAGCACGAAGTTGTTAAAGTTAAAGGCGAAGCTG                     >  SRR4302535.4304237/1‑94 (MQ=60)
                                                                         TAACCGGCTGGCGTGGTCGCGCCAAGACGATCCGGGCCGCCGTTGGTTAACAGTTGAATCAGCACGAAGTTGTTAAAGTTAAAGGCGAAGCTGG                    <  SRR4302535.4115338/94‑1 (MQ=60)
                                                                         TAACCGGCTGGCGTGGTCGCGCCAAGACGATCCGGGCCGCCGGTGGTTAACAGGTGAATCAGCACGCAGCTGTTAAAGTTAAAGGCGAAGCTAG                    >  SRR4302535.2486472/1‑94 (MQ=60)
                                                                          AACCGGCTGGCGTGGTCGCGCCAAGACGATCCGGGCCGCCGTTGGTTAACAGTTGAATCAGCACGAAGTTGTTAAAGTTAAAGGCGAAGCTGGC                   <  SRR4302535.1072235/94‑1 (MQ=60)
                                                                           ACCGGCTGGCGTGGTCGCGCCAAGACGATCCGGGCCGCCGTTGGTTAACAGTTGAATCAGCACGAAGTTGTTAAAGTTAAAGGCGAAGCTGGCG                  >  SRR4302535.1826923/1‑94 (MQ=60)
                                                                            CCGGCTGGCGTGGTCGCGCCAAGACGATCCGGGCCGCCGTTGGTTAACAGTTGAATCAGCACGAAGTTGTTAAAGTTAAAGGCGAAGCTGGCGA                 >  SRR4302535.2760359/1‑94 (MQ=60)
                                                                              GGCTGGCGTGGTCGCGCCAAGACGATCCGGGCCGCCGTTGGTTAACAGTTGAATCAGCACGAAGTTGTTAAAGTTAAAGGCGAAGCTGGCGATC               >  SRR4302535.279120/1‑94 (MQ=60)
                                                                                CTGGCGTGGTCGCGCCAAGACGATCCGGGCCGCCGTTGGTTAACAGTTGAATCAGCACGAAGTTGTTAAAGTTAAAGGCGAAGCTGGCGATCAT             <  SRR4302535.209175/94‑1 (MQ=60)
                                                                                CTGGCGTGGTCGCGCCAAGACGATCCGGGCCGCCGTTGGTTAACAGTTGAATCAGCACGAAGTTGTTAAAGTTAAAGGCGAAGCTGGCGATCAT             >  SRR4302535.805393/1‑94 (MQ=60)
                                                                                  GGCGTGGTCGCGCCAAGACGATCCGGGCCGCCGTTGGTTAACAGTTGAATCAGCACGAAGTTGTTAAAGTTAAAGGCGAAGCTGGCGATCATCA           <  SRR4302535.4621457/94‑1 (MQ=60)
                                                                                  GGCGTGGTCGCGCCAAGACGATCCGGGCCGCCGTTGGTTAACAGTTGAATCAGCACGAAGTTGTTAAAGTTAAAGGCGAAGCTGGCGATCATCA           >  SRR4302535.1625315/1‑94 (MQ=60)
                                                                                   GCGTGGTCGCGCCAAGACGATCCGGGCCGCCGTTGGTTAACAGTTGAATCAGCACGAAGTTGTTAAAGTTAAAGGCGAAGCTGGCGATCATCAG          <  SRR4302535.2008083/94‑1 (MQ=60)
                                                                                   GCGTGGTCGCGCCAAGACGATCCGGGCCGCCGTTGGTTAACAGTTGAATCAGCACGAAGTTGTTAAAGTTAAAGGCGAAGCTGGCGATCATCAG          <  SRR4302535.2732018/94‑1 (MQ=60)
                                                                                    CGTGGTCGCGCCAAGACGATCCGGGCCGCCGTTGGTTAACAGTTGAATCAGCACGAAGTTGTTAAAGTTAAAGGCGAAGCTGGCGATCATCAGC         <  SRR4302535.3027645/94‑1 (MQ=60)
                                                                                    CGTGGTCGCGCCAAGACGATCCGGGCCGCCGTTGGTTAACAGTTGAATCAGCACGAAGTTGTTAAAGTTAAAGGCGAAGCTGGCGATCATCAGC         <  SRR4302535.4166041/94‑1 (MQ=60)
                                                                                     GTGGTCGCGCCAAGACGATCCGGGCCGCCGTTGGTTAACAGTTGAATCAGCACGAAGTTGTTATAGTTAAAGGCGAAGCTGGCGATCATCAGCG        >  SRR4302535.3417422/1‑94 (MQ=60)
                                                                                     GTGGTCGCGCCAAGACGATCCGGGCCGCCGTTGGTTAACAGTTGAATCAGCACGAAGTTGTTAAAGTTAAAGGCGAAGCTGGCGATCATCAGCG        >  SRR4302535.2039432/1‑94 (MQ=60)
                                                                                     GTGGTCGCGCCAAGACGATCCGGGCCGCCGTTGGTTAACAGTTGAATCAGCACGAAGTTGTTAAAGTTAAAGGCGAAGCTGGCGATCATCAGCG        <  SRR4302535.4099581/94‑1 (MQ=60)
                                                                                        GTCGCGCCAAGACGATCCGGGCCGCCGTTGGTTAACAGTTGAATCAGCACGAAGTTGTTAAAGTTAAAGGCGAAGCTGGCGATCATCAGCGGCG     <  SRR4302535.2937857/94‑1 (MQ=60)
                                                                                          CGCGCCAAGACGATCCGGGCCGCCGTTGGTTAACAGTTGAATCAGCACGAAGTTGTTAAAGTTAAAGGCGAAGCTGGCGATCATCAGCGGCGTC   >  SRR4302535.1106489/1‑94 (MQ=60)
                                                                                           GCGCCAAGACGATCCGGGCCGCCGTTGGTTAACAGTTGAATCAGCACGAAGTTGTTAAAGTTAAAGGCGAAGCTGGCGATCATCAGCGGCGTCA  <  SRR4302535.4042182/94‑1 (MQ=60)
                                                                                            |                                                                                            
CGCCAGACCGAAGTCCTGACCCCCGCCGCCTTCAAAAGCGATGCGGTAGGTGTAGTTAACAAGCAGGTCGGTATAACCGGCTGGCGTGGTCGTGCCAAGACGATCCGGGCCGCCGTTGGTTAACAGTTGAATCAGCACGAAGTTGTTAAAGTTAAAGGCGAAGCTGGCGATCATCAGCGGCGTCA  >  NC_000913/4241194‑4241378

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑