Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1873	183.1	9376148	98.2%	9207377	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,245,705	T→C	G52G (GGT→GGC)	lamB →	phage lambda receptor protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,245,705	0	T	C	99.1%	671.4 / ‑4.8	216	G52G (GGT→GGC)	lamB	phage lambda receptor protein
Reads supporting (aligned to +/- strand): ref base T (0/2); new base C (84/130); total (84/132)
Fisher's exact test for biased strand distribution p-value = 5.22e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01

Only 100 of 224 total aligned reads displayed.

GGCAATGGCTGTTGATTTCCACGGCTATGCACGTTCCGGTATTGGTTGGACAGGTAGCGGCGGTGAACAACAGTGTTTCCAGACTACCGGTGCTCAAAGTAAATACCGTCTTGGCAACGAATGTGAAACTTATGCTGAATTAAAATTGGGTCAGGAAGTGTGGAAAGAGGGCGATAAGAG  >  NC_000913/4245615‑4245794

     tGGCTGTTGATTTCCACGGCTATGCACGTTCCGGTATTGGTTGGACAGGTAGCGGCGGTGAACAACAGTGTTTCCAGACTACCGGCGCTCaaa                                                                                    <  2:2195180/93‑1 (MQ=255)

     tGGCTGTTGATTTCCACGGCTATGCACGTTCCGGTATTGGTTGGACAGGTAGCGGCGGTGAACAACAGTGTTTCCAGACTACCGGCGCTCaaa                                                                                    <  1:2442002/93‑1 (MQ=255)

      ggCTGTTGATTTCCACGGCTATGCACGTTCCGGTATTGGTTGGACAGGTAGCGGCGGTGAACAACAGTGTTTCCAGACTACCGGCGCTCAAAg                                                                                   <  1:72063/93‑1 (MQ=255)

      ggCTGTTGATTTCCACGGCTATGCACGTTCCGGTATTGGTTGGACAGGTAGCGGCGGTGAACAACAGTGTTTCCAGACTACCGGCGCTCAAAg                                                                                   <  2:1143102/93‑1 (MQ=255)

      ggCTGTTGATTTCCACGGCTATGCACGTTCCGGTATTGGTTGGACAGGCAGCGGCGGGGAACAACAGTGTTTCCCCACTACCGGCGCTCAAAg                                                                                   >  1:1139036/1‑93 (MQ=255)

         tGTTGATTTCCACGGCTATGCACGTTCCGGTATTGGTTGGACAGGTAGCGGCGGTGAACAACAGTGTTTCCAGACTACCGGCGCTCAAAGTaa                                                                                >  2:1089646/1‑93 (MQ=255)

         tGTTGATTTCCACGGCTATGCACGTTCCGGTATTGGTTGGACAGGTAGCGGCGGTGAACAACAGTGTTTCCAGACTACCGGCGCTCAAAGTaa                                                                                <  1:1832109/93‑1 (MQ=255)

         tGTTGATTTCCACGGCTATGCACGTTCCGGTATTGGTTGGACAGGTAGCGGCGGTGAACAACAGTGTTTCCAGACTACCGGCGCTCAAAGTaa                                                                                >  2:3234194/1‑93 (MQ=255)

          gTTGATTTCCACGGCTATGCACGTTCCGGTATTGGTTGGACAGGTAGCGGCGGTGAACAACAGTGTTTCCAGACTACCGGCGCTCAAAGTaaa                                                                               <  1:3114108/93‑1 (MQ=255)

          gTTGATTTCCACGGCTATGCACGTTCCGGTATTGGTTGGACAGGTAGCGGCGGTGAACAACAGTGTTTCCAGACTACCGGCGCTCAAAGTaaa                                                                               <  2:2768611/93‑1 (MQ=255)

          gTTGATTTCCACGGCTATGCACGTTCCGGTATTGGTTGGACAGGTAGCGGCGGTGAACAACAGTGTTTCCAGACTACCGGCGCTCAAAGTaaa                                                                               >  2:3190085/1‑93 (MQ=255)

           ttGATTTCCACGGCTATGCACGTTCCGGTATTGGTTGGACAGGTAGCGGCGGTGAACAACAGTGTTTCCAGACTACCGGCGCTCAAAGTAAAt                                                                              <  2:3457053/93‑1 (MQ=255)

            tGATTTCCACGGCTATGCACGTTCCGGTATTGGTTGGACAGGTAGCGGCGGTGAACAACAGTGTTTCCAGACTACCGGCGCTCAAAGTAAATa                                                                             <  1:4331714/93‑1 (MQ=255)

            tGATTTCCACGGCTATGCACGTTCCGGTATTGGTTGGACAGGTAGCGGCGGTGAACAACAGTGTTTCCAGACTACCGGCGCTCAAAGTAAATa                                                                             <  1:1484636/93‑1 (MQ=255)

            tGATTTCCACGGCTATGCACGTTCCGGTATTGGTTGGACAGGTAGCGGCGGTGAACAACAGTGTTTCCAGACTACCGGCGCTCAAAGTAAATa                                                                             <  1:1534871/93‑1 (MQ=255)

             gATTTCCACGGCTATGCACGTTCCGGTATTGGTTGGACAGGTAGCGGCGGTGAACAACAGTGTTTCCAGACTACCGGCGCTCAAAGTAAATAc                                                                            <  2:3372342/93‑1 (MQ=255)

             gATTTCCACGGCTATGCACGTTCCGGTATTGGTTGGACAGGTAGCGGCGGTGAACAACAGTGTTTCCAGACTACCGGCGCTCAAAGTAAATAc                                                                            >  2:446478/1‑93 (MQ=255)

             gATTTCCACGGCTATGCACGTTCCGGTATTGGTTGGACAGGTAGCGGCGGTGAACAACAGTGTTTCCAGACTACCGGCGCTCAAAGTAAATAc                                                                            >  2:838218/1‑93 (MQ=255)

               tttCCACGGCTATGCACGTTCCGGTATTGGTTGGACAGGTAGCGGCGGTGAACAACAGTGTTTCCAGACTACCGGCGCTCAAAGTAAATACCg                                                                          <  2:2386552/93‑1 (MQ=255)

                ttCCACGGCTATGCACGTTCCGGTATTGGTTGGACAGGTAGCGGCGGTGAACAACAGTGTTTCCAGACTACCGGCGCTCAAAGTAAATACCGt                                                                         >  2:2686676/1‑93 (MQ=255)

                  ccACGGCTATGCACGTTCCGGTATTGGTTGGACAGGTAGCGGCGGTGAACAACAGTGTTTCCAGACTACCGGCGCTCAAAGTAAATACCGTCt                                                                       <  1:3077397/93‑1 (MQ=255)

                   cACGGCTATGCACGTTCCGGTATTGGTTGGACAGGTAGCGGCGGTGAACAACAGTGTTTCCAGACTACCGGCGCTCAAAGTAAATACCGTCtt                                                                      <  1:3353290/93‑1 (MQ=255)

                   cACGGCTATGCACGTTCCGGTATTGGTTGGACAGGTAGCGGCGGTGAACAACAGTGTTTCCAGACTACCGGCGCTCAAAGTAAATACCGTCtt                                                                      <  2:1991481/93‑1 (MQ=255)

                    aCGGCTATGCACGTTCCGGTATTGGTTGGACAGGTAGCGGCGGTGAACAACAGTGTTTCCAGACTACCGGCGCTCAAAGTAAATACCGTCTTg                                                                     >  1:2246045/1‑93 (MQ=255)

                    aCGGCTATGCACGTTCCGGTATTGGTTGGACAGGTAGCGGCGGTGAACAACAGTGTTTCCAGACTACCGGCGCTCAAAGTAAATACCGTCTTg                                                                     >  2:4411631/1‑93 (MQ=255)

                       gCTATGCACGTTCCGGTATTGGTTGGACAGGTAGCGGCGGTGAACAACAGTGTTTCCAGACTACCGGCGCTCAAAGTAAATACCGTCTTGGCa                                                                  >  2:4440585/1‑93 (MQ=255)

                        cTATGCACGTTCCGGTATTGGTTGGACAGGTAGCGGCGGTGAACAACAGTGTTTCCAGACTACCGGCGCTCAAAGTAAATACCGTCTTGGCaa                                                                 <  2:3840089/93‑1 (MQ=255)

                        cTAGGCACGTTCCGGTATTGGTTGGACAGGTAGCGGCGGTGAACAACAGTGTTTCCAGACTACCGGCGCTCAAAGTAAATACCGTCTTGGCaa                                                                 <  2:689129/93‑1 (MQ=255)

                           tGCACGTTCCGGTATTGGTTGGACAGGTAGCGGCGGTGAACAACAGTGTTTCCAGACTACCGGCGCTCAAAGTAAATACCGTCTTGGCAACGa                                                              >  2:3643509/1‑93 (MQ=255)

                            gCACGTTCCGGTATTGGTTGGACAGGTAGCGGCGGTGAACAACAGTGTTTCCAGACTACCGGCGCTCAAAGTAAATACCGTCTTGGCAACGaa                                                             >  2:42345/1‑93 (MQ=255)

                             cACGTTCCGGTATTGGTTGGACAGGTAGCGGCGGTGAACAACAGTGTTTCCAGACTACCGGCGCTCAAAGTAAATACCGTCTTGGCAACGAAt                                                            <  2:163899/93‑1 (MQ=255)

                              aCGTTCCGGTATTGGTTGGACAGGTAGCGGCGGTGAACAACAGTGTTTCCAGACTACCGGCGCTCAAAGTAAATACCGTCTTGGCAACGAAtg                                                           <  1:4341044/93‑1 (MQ=255)

                              aCGTTCCGGTATTGGTTGGACAGGTAGCGGCGGTGAACAACAGTGTTTCCAGACTACCGGCGCTCAAAGTAAATACCGTCTTGGCAACGAAtg                                                           <  1:1947086/93‑1 (MQ=255)

                                gTTCCGGTATTGGTTGGACAGGTAGCGGCGGTGAACAACAGTGTTTCCAGACTACCGGCGCTCAAAGTAAATACCGTCTTGGCAACGAAtgtg                                                         <  2:2811217/93‑1 (MQ=255)

                                gTTCCGGTATTGGTTGGACAGGTAGCGGCGGTGAACAACAGAGTTTCCAGACTACCGGCGCTCAAAGTAAATACCGTCTTCGCAACGAAtgtg                                                         >  1:1231219/1‑93 (MQ=255)

                                 ttCCGGTATTGGTTGGACAGGTAGCGGCGGTGAACAACAGTGTTTCCAGACTACCGGCGCTCAAAGTAAATACCGTCTTGGCAACGAATGTGa                                                        <  2:3863906/93‑1 (MQ=255)

                                    cGGTATTGGTTGGACAGGTAGCGGCGGTGAACAACAGTGTTTCCAGACTACCGGCGCTCAAAGTAAATACCGTCTTGGCACCGAATGTGAAAc                                                     <  2:3596989/93‑1 (MQ=255)

                                     ggTATTGGTTGGACAGGTAGCGGCGGTGAACAACAGTGTTTCCAGACTACCGGCGCTCAAAGTAAATACCGTCTTGGCAACGAATGTGAAACt                                                    >  1:4083372/1‑93 (MQ=255)

                                     ggTATTGGTTGGACAGGTAGCGGCGGTGAACAACAGTGTTTCCAGACTACCGGCGCTCAAAGTAAATACCGTCTTGGCAACGAATGTGAAACt                                                    <  2:2079326/93‑1 (MQ=255)

                                      gTATTGGTTGGACAGGTAGCGGCGGTGAACAACAGTGTTTCCAGACTACCGGCGCTCAAAGTAAATACCGTCTTGGCAACGAATGTGAAACtt                                                   <  2:508553/93‑1 (MQ=255)

                                       tATTGGTTGGACAGGTAGCGGCGGTGAACAACAGTGTTTCCAGACTACCGGCGCTCAAAGTAAATACCGTCTTGGCAACGAATGTGAAACTTa                                                  <  2:3883680/93‑1 (MQ=255)

                                       tATTGGTTGGACAGGTAGCGGCGGTGAACAACAGTGTTTCCAGACTACCGGCGCTCAAAGTAAATACCGTCTTGGCAACGAATGTGAAACTTa                                                  <  1:768498/93‑1 (MQ=255)

                                        aTTGGTTGGACAGGTAGCGGCGGTGAACAACAGTGTTTCCAGACTACCGGCGCTCAAAGTAAATACCGTCTTGGCAACGAATGTGAAACTTAt                                                 <  1:1057930/93‑1 (MQ=255)

                                        aTTGGTTGGACAGGTAGCGGCGGTGAACAACAGTGTTTCCAGACTACCGGCGCTCAAAGTAAATACCGTCTTGGCAACGAATGTGAAACTTAt                                                 >  1:3542459/1‑93 (MQ=255)

                                        aTTGGTTGGACAGGTAGCGGCGGTGAACAACAGTGTTTCCAGACTACCGGCGCTCAAAGTAAATACCGTCTTGGCAACGAATGTGAAACTTAt                                                 <  2:1507393/93‑1 (MQ=255)

                                          tggttggACAGGTAGCGGCGGTGAACAACAGTGTTTCCAGACTAACGGCGCTCAAAGTAAATACCGTCTTGGCAACGAATGTGAAACTTATgg                                               >  2:1241531/1‑92 (MQ=255)

                                           ggttggACAGGTAGCGGCGGTGAACAACAGTGTTTCCAGACTACCGGCGCTCAAAGTAAATACCGTCTTGGCAACGAATGTGAAACTTATGCt                                              <  2:455519/93‑1 (MQ=255)

                                           ggttggACAGGTAGCGGCGGTGAACAACAGTGTTTCCAGACTACCGGCGCTCAAAGTAAATACCGTCTTGGCAACGAATGTGAAACTTATGCt                                              >  2:3699311/1‑93 (MQ=255)

                                           ggttggACAGGTAGCGGCGGTGAACAACAGTGTTTCCAGACTACCGGCGCTCAAAGTAAATACCGTCTTGGCAACGAATGTGAAACTTATGCt                                              >  1:525753/1‑93 (MQ=255)

                                                  cAGGTAGCGGCGGTGAACAACAGTGTTTCCAGACTACCGGCGCTCAAAGTAAATACCGTCTTGGCAACGAATGTGAAACTTATGCTGAATTaa                                       <  2:4249894/93‑1 (MQ=255)

                                                  cAGGTAGCGGCGGTGAACAACAGTGTTTCCAGACTACCGGCGCTCAAAGTAAATACCGTCTTGGCAACGAATGTGAAACTTATGCTGAATTaa                                       <  1:17645/93‑1 (MQ=255)

                                                     gTAGCGGCGGTGAACAACAGTGTTTCCAGACTACCGGCGCTCAAAGTAAATACCGTCTTGGCAACGAATGTGAAACTTATGCTGAATTAAAAt                                    >  1:60646/1‑93 (MQ=255)

                                                      tAGCGGCGGTGAACAACAGTGTTTCCAGACTACCGGCGCTCAAAGTAAATACCGTCTTGGCAACGAATGTGAAACTTATGCTGAATTAAAAtt                                   <  1:2075301/93‑1 (MQ=255)

                                                      tAGCGGCGGTGAACAACAGTGTTTCCAGACTACCGGCGCTCAAAGTAAATACCGTCTTGGCAACGAATGTGAAACTTATGCTGAATTAAAAtt                                   <  2:3509704/93‑1 (MQ=255)

                                                        gcggcgGTGAACAACAGTGTTTCCAGACTACCGGCGCTCAAAGTAAATACCGTCTTGGCAACGAATGTGAAACTTATGCTGAATTAAAATTgg                                 >  2:2626941/1‑93 (MQ=255)

                                                         cggcggTGAACAACAGTGTTTCCAGACTACCGGCGCTCAAAGTAAATACCGTCTTGGCAACGAATGTGAAACTTATGCTGAATTAAAATTggg                                <  1:3452422/93‑1 (MQ=255)

                                                          ggcggTGAACAACAGTGTTTCCAGACTACCGGCGCTCAAAGTAAATGCCGTCTTGGCAACGAATGTGAAACTTATGCTGAATTAAAATTGGGt                               <  2:829841/93‑1 (MQ=255)

                                                           gcggTGAACAACAGTGTTTCCAGACTACCGGCGCTCAAAGTAAATACCGTCTTGGCAACGAATGTGAAACTTATGCTGAATTAAAATTGGGTc                              >  1:4493200/1‑93 (MQ=255)

                                                             ggTGAACAACAGTGTTTCCAGACTACCGGCGCTCAAAGTAAATACCGTCTTGGCAACGAATGTGAAACTTATGCTGAATTAAAATTGGGTCAg                            <  2:131761/93‑1 (MQ=255)

                                                             ggTGAACAACAGTGTTTCCAGACTACCGGCGCTCAAAGTAAATACCGTCTTGGCAACGAATGTGAAACTTATGCTGAATTAAAATTGGGTCAg                            <  1:3844776/93‑1 (MQ=255)

                                                              gTGAACAACAGTGTTTCCAGACTACCGGCGCTCAAAGTAAATACCGTCTTGGCAACGAATGTGAAACTTATGCTGAATTAAAATTGGGTCAgg                           <  1:4018593/93‑1 (MQ=255)

                                                               tGAACAACAGTGTTTCCAGACTACCGGCGCTCAAAGTAAATACCGTCTTGGCAACGAATGTGAAACTTATGCTGAATTAAAATTGGGTCAGGa                          <  2:2491119/93‑1 (MQ=255)

                                                                gAACAACAGTGTTTCCAGACTACCGGCGCTCAAAGTAAATACCGTCTTGGCAACGAATGTGAAACTTATGCTGAATTAAAATTGGGTCAGGaa                         <  2:1371355/93‑1 (MQ=255)

                                                                 aacaacAGTGTTTCCAGACTACCGGCGCTCAAAGTAAATACCGTCTTGGCAACGAATGTGAAACTTATGCTGAATTAAAATTGGGTCAGGAAg                        <  2:3696962/93‑1 (MQ=255)

                                                                 aacaacAGTGTTTCCAGACTACCGGCGCTCAAAGTAAATACCGTCTTGGCAACGAATGTGAAACTTATGCTGAATTAAAATTGGGTCAGGAAg                        <  2:467140/93‑1 (MQ=255)

                                                                  acagcaGTGTTTCCAGACTACCGGTGCTCAAAGTAAATACCGTCTTGGCAACGAATGTGAAACTTATGCTGAATTAAAATTGGGTCAGGAAgt                       <  1:1174441/93‑1 (MQ=255)

                                                                  acaacaGTGTTTCCAGACTACCGGCGCTCAAAGTAAATACCGTCTTGGCAACGAATGTGAAACTTATGCTGAATTAAAATTGGGTCAGGAAgg                       >  2:1897082/1‑92 (MQ=255)

                                                                   caacaGTGTTTCCAGACTACCGGCGCTCAAAGTAAATACCGTCTTGGCAACGAATGTGAAACTTATGCTGAATTAAAATTGGGTCAGGAAgtg                      >  2:3963193/1‑93 (MQ=255)

                                                                     acaGTGTTTCCAGACTACCGGCGCTCAAAGTAAATACCGTCTTGGCAACGAATGTGAAACTTATGCTGAATTAAAATTGGGTCAGGAAGtgtg                    <  1:4582088/93‑1 (MQ=255)

                                                                     acaGTGTTTCCAGACTACCGGCGCTCAAAGTAAATACCGTCTTGGCAACGAATGTGAAACTTATGCTGAATTAAAATTGGGTCAGGAAGtgtg                    <  1:4638484/93‑1 (MQ=255)

                                                                     acaGTGTTTCCAGACTACCGGCGCTCAAAGTAAATACCGTCTTGGCAACGAATGTGAAACTTATGCTGAATTAAAATTGGGTCAGGAAGtgtg                    >  1:1753162/1‑93 (MQ=255)

                                                                     acaGTGTTTCCAGACTACCGGCGCTCAAAGTAAATACCGTCTTGGCAACGAATGTGAAACTTATGCTGAATTAAAATTGGGTCAGGAAGtgtg                    <  2:4626084/93‑1 (MQ=255)

                                                                      caGTGTTTCCAGACTACCGGCGCTCAAAGTAAATACCGTCTTGGCAACGAATGTGAAACTTATGCTGAATTAAAATTGGGTCAGGAAGTGTgg                   >  2:2882634/1‑93 (MQ=255)

                                                                       aGTGTTTCCAGACTACCGGCGCTCAAAGTAAATACCGTCTTGGCAACGAATGTGAAACTTATGCTGAATTAAAATTGGGTCAGGAAGTGTGGa                  >  2:3016274/1‑93 (MQ=255)

                                                                       aGTGTTTCCAGACTACCGGCGCTCAAAGTAAATACCGTCTTGGCAACGAATGTGAAACTTATGCTGAATTAAAATTAGGTCAGGAAGTGTGGa                  >  2:1957124/1‑93 (MQ=255)

                                                                        gtgtTTCCAGACTACCGGCGCTCAAAGTAAATACCGTCTTGGCAACGAATGTGAAACTTATGCTGAATTAAAATTGGGTCAGGAAGTGTGGaa                 >  1:3516361/1‑93 (MQ=255)

                                                                           tttCCAGACTACCGGCGCTCAAAGTAAATACCGTCTTGGCAACGAATGTGAAACTTATGCTGAATTAAAATTGGGTCAGGAAGTGTGGAAaga              <  2:2757161/93‑1 (MQ=255)

                                                                           tttCCAGACTACCGGCGCTCAAAGTAAATACCGTCTTGGCAACGAATGTGAAACTTATGCTGAATTAAAATTGGGTCAGGAAGTGTGGAAaga              <  1:4457570/93‑1 (MQ=255)

                                                                            ttCCAGACTACCGGCGCTCAAAGTAAATTCCGTCTTGGCAACGAATGTGAAACTTATGCTGAATTAAAATTGGGTCAGGAAGTGTGGAAagag             <  2:2158638/93‑1 (MQ=255)

                                                                            ttCCAGACTACCGGCGCTCAAAGTAAATACCGTCTTGGCAACGAATGTGAAACTTATGCTGAATTAAAATTGGGTCAGGAAGTGTGGAAagag             <  1:3691451/93‑1 (MQ=255)

                                                                             tCCAGACTACCGGCGCTCAAAGTAAATACCGTCTTGGCAACGAATGTGAAACTTATGCTGAATTAAAATTGGGTCAGGAAGTGTGGAAAGAgg            <  2:1759503/93‑1 (MQ=255)

                                                                             tCCAGACTACCGGCGCTCAAAGTAAATACCGTCTTGGCAACGAATGTGAAACTTATGCTGAATTAAAATTGGGTCAGGAAGTGTGGAAAGAgg            <  1:1189279/93‑1 (MQ=255)

                                                                             tCCAGACTACCGGCGCTCAAAGTAAATACCGTCTTGGCAACGAATGTGAAACTTATGCTGAATTAAAATTGGGTCAGGAAGTGTGGAAAGAgg            <  1:3904478/93‑1 (MQ=255)

                                                                              ccAGACTACCGGCGCTCAAAGTAAATACCGTCTTGGCAACGAATGTGAAACTTATGCTGAATTAAAATTGGGTCAGGAAGTGTGGAAAGAggg           <  2:3060106/93‑1 (MQ=255)

                                                                               cAGACTACCGGCGCTCAAAGTAAATACCGTCTTGGCAACGAATGTGAAACTTATGCTGAATTAAAATTGGGTCAGGAAGTGTGGAAAGAGGGc          >  2:710751/1‑93 (MQ=255)

                                                                               cAGACTACCGGCGCTCAAAGTAAATACCGTCTTGGCAACGAATGTGAAACTTATGCTGAATTAAAATTGGGTCAGGAAGTGTGGAAAGAGGGc          <  1:1021428/93‑1 (MQ=255)

                                                                               cAGACTACCGGCGCTCAAAGTAAATACCGTCTTGGCAACGAATGTGAAACTTATGCTGAATTAAAATTGGGTCAGGAAGTGTGGAAAGAGGGc          >  1:3599198/1‑93 (MQ=255)

                                                                                aGACTACCGGCGCTCAAAGTAAATACCGTCTTGGCAACGAATGTGAAACTTATGCTGAATTACAATTGGGTCAGGAAGTGTGGAAAGAggcga         >  1:3251429/1‑90 (MQ=255)

                                                                                aGACTACCGGCGCTCAAAGTAAATACCGTCTTGGCAACGAATGTGAAACTTATGCTGAATTAAAATTGGGTCAGGAAGTGTGGAAAGAGGGCg         >  1:2831632/1‑93 (MQ=255)

                                                                                aGACTACCGGCGCTCAAAGTAAATACCGTCTTGGCAACGAATATGAAACTTATGCTGAATTAAAATTGGGTCAGGAAGTGTGGAAAGAGGGCg         >  1:2518044/1‑93 (MQ=255)

                                                                                aGACTACCGGCGCTCAAAGGAAATACCGTCTTGGCAACGAATGTGAAACTTATGCTGAATTAAAATTTGGGTTGGCAGAGTGGAAAGAGGGCg         >  2:3777311/1‑93 (MQ=255)

                                                                                 gACTACCGGCGCTCAAAGTAAATACCGTCTTGGCAACGAATGTGAAACTTATGCTGAATTAAAATTGGGTCAGGAAGTGTGGAAAGAGGGCGa        >  2:4070322/1‑93 (MQ=255)

                                                                                  aCTACCGGCGCTCAAAGTAAATACCGTCTTGGCAACGAATGTGAAACTTATGCTGAATTAAAATTGGGTCAGGAAGTGTGGAAAGAGGGCGAt       <  2:4128139/93‑1 (MQ=255)

                                                                                   cTACCGGCGCTCAAAGTAAATACCGTCTTGGCAACGAATGTGAAACTTATGCTGAATTAAAATTGGGTCAGGAAGTGTGGAAAGAGGGCGATa      <  1:229372/93‑1 (MQ=255)

                                                                                   cTACCGGCGCTCAAAGTAAATACCGTCTTGGCAACGAATGTGAAACTTATGCTGAATTAAAATTGGGTCAGGAAGTGTGGAAAGAGGGCGATa      >  1:2947881/1‑93 (MQ=255)

                                                                                    tACCGGCGCTCAAAGTAAATACCGTCTTGGCAACGAATGTGAAACTTATGCTGAATTAAAATTGGGTCAGGAAGTGTGGAAAGAGGGCGATaa     >  2:4390822/1‑93 (MQ=255)

                                                                                    tACCGGCGCTCAAAGTAAATACCGTCTTGGCAACGAATGTGAAACTTATGCTGAATCAAAATTGGGTCAGGAAGTGTGGAAAGAGGGCGATaa     <  2:4103258/93‑1 (MQ=255)

                                                                                     aCCGGCGCTCAAAGTAAATACCGTCTTGGCAACGAATGTGAAACTTATGCTGAATTAAAATTGGGTCAGGAAGTGTGGAAAGAGGGCGATAag    >  1:1279179/1‑93 (MQ=255)

                                                                                     aCCGGCGCTCAAAGTAAATACCGTCTTGGCAACGAATGTGAAACTTATGCTGAATTAAAATTGGGTCAGGAAGTGTGGAAAGAGGGCGATAag    >  2:2308504/1‑93 (MQ=255)

                                                                                       cGGCGCTCAAAGTAAATACCGTCTTGGCAACGAATGTGAAACTTATGCTGAATTAAAATTGGGTCAGGAAGTGTGGAAAGAGGGCGATAagag  >  1:3953791/1‑93 (MQ=255)

GGCAATGGCTGTTGATTTCCACGGCTATGCACGTTCCGGTATTGGTTGGACAGGTAGCGGCGGTGAACAACAGTGTTTCCAGACTACCGGTGCTCAAAGTAAATACCGTCTTGGCAACGAATGTGAAACTTATGCTGAATTAAAATTGGGTCAGGAAGTGTGGAAAGAGGGCGATAAGAG  >  NC_000913/4245615‑4245794

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

GGCAATGGCTGTTGATTTCCACGGCTATGCACGTTCCGGTATTGGTTGGACAGGTAGCGGCGGTGAACAACAGTGTTTCCAGACTACCGGTGCTCAAAGTAAATACCGTCTTGGCAACGAATGTGAAACTTATGCTGAATTAAAATTGGGTCAGGAAGTGTGGAAAGAGGGCGATAAGAGCTT  >  NC_000913/4245615‑4245797

GGCAATGGCTGTTGATTTCCACGGCTATGCACGTTCCGGTATTGGTTGGACAGGTAGCGGCGGTGAACAACAGTGTTTCCAGACTACCGGCGCT                                                                                           <  SRR4302535.2283911/94‑1 (MQ=60)

   AATGGCTGTTGATTTCCACGGCTATGCACGTTCCGGTATTGGTTGGACAGGTAGCGGCGGTGAACAACAGTGTTTCCAGACTACCGGCGCTCAA                                                                                        >  SRR4302535.3965676/1‑94 (MQ=60)

     TGGCTGTTGATTTCCACGGCTATGCACGTTCCGGTATTGGTTGGACAGGTAGCGGCGGTGAACAACAGTGTTTCCAGACTACCGGCGCTCAAAG                                                                                      <  SRR4302535.2459594/94‑1 (MQ=60)

     TGGCTGTTGATTTCCACGGCTATGCACGTTCCGGTATTGGTTGGACAGGTAGCGGCGGTGAACAACAGTGTTTCCAGACTACCGGCGCTCAAAG                                                                                      <  SRR4302535.3520899/94‑1 (MQ=60)

     TGGCTGTTGATTTCCACGGCTATGCACGTTCCGGTATTGGTTGGACAGGCAGCGGCGGGGAACAACAGTGTTTCCCCACTACCGGCGCTCAAAG                                                                                      >  SRR4302535.1147155/1‑94 (MQ=60)

      GGCTGTTGATTTCCACGGCTATGCACGTTCCGGTATTGGTTGGACAGGTAGCGGCGGTGAACAACAGTGTTTCCAGACTACCGGCGCTCAAAGT                                                                                     <  SRR4302535.72589/94‑1 (MQ=60)

         TGTTGATTTCCACGGCTATGCACGTTCCGGTATTGGTTGGACAGGTAGCGGCGGTGAACAACAGTGTTTCCAGACTACCGGCGCTCAAAGTAAA                                                                                  <  SRR4302535.1845329/94‑1 (MQ=60)

         TGTTGATTTCCACGGCTATGCACGTTCCGGTATTGGTTGGACAGGTAGCGGCGGTGAACAACAGTGTTTCCAGACTACCGGCGCTCAAAGTAAA                                                                                  <  SRR4302535.3684526/94‑1 (MQ=60)

          GTTGATTTCCACGGCTATGCACGTTCCGGTATTGGTTGGACAGGTAGCGGCGGTGAACAACAGTGTTTCCAGACTACCGGCGCTCAAAGTAAAT                                                                                 <  SRR4302535.3135787/94‑1 (MQ=60)

           TTGATTTCCACGGCTATGCACGTTCCGGTATTGGTTGGACAGGTAGCGGCGGTGAACAACAGTGTTTCCAGACTACCGGCGCTCAAAGTAAATA                                                                                >  SRR4302535.1032222/1‑94 (MQ=60)

           TTGATTTCCACGGCTATGCACGTTCCGGTATTGGTTGGACAGGTAGCGGCGGTGAACAACAGTGTTTCCAGACTACCGGCGCTCAAAGTAAATA                                                                                <  SRR4302535.461172/94‑1 (MQ=60)

            TGATTTCCACGGCTATGCACGTTCCGGTATTGGTTGGACAGGTAGCGGCGGTGAACAACAGTGTTTCCAGACTACCGGCGCTCAAAGTAAATAC                                                                               <  SRR4302535.4361338/94‑1 (MQ=60)

            TGATTTCCACGGCTATGCACGTTCCGGTATTGGTTGGACAGGTAGCGGCGGTGAACAACAGTGTTTCCAGACTACCGGCGCTCAAAGTAAATAC                                                                               <  SRR4302535.1495258/94‑1 (MQ=60)

            TGATTTCCACGGCTATGCACGTTCCGGTATTGGTTGGACAGGTAGCGGCGGTGAACAACAGTGTTTCCAGACTACCGGCGCTCAAAGTAAATAC                                                                               >  SRR4302535.3924738/1‑94 (MQ=60)

            TGATTTCCACGGCTATGCACGTTCCGGTATTGGTTGGACAGGTAGCGGCGGTGAACAACAGTGTTTCCAGACTACCGGCGCTCAAAGTAAATAC                                                                               <  SRR4302535.1545929/94‑1 (MQ=60)

            TGATTTCCACGGCTATGCACGTTCCGGTATTGGTTGGACAGGTAGCGGCGGTGAACAACAGTGTTTCCAGACTACCGGCGCTCAAAGTAAATAC                                                                               <  SRR4302535.2220385/94‑1 (MQ=60)

             TATTTCCACGGCTATGCACGTTCCGGTATTGGTTGGACAGGTAGCGGCGGTGAACAACAGTGTTTCCAGACTACCGGCGCTCAAAGTAAATACC                                                                              <  SRR4302535.413408/94‑1 (MQ=60)

             GATTTCCACGGCTATGCACGTTCCGGTATTGGTTGGACAGGTAGCGGCGGTGAACAACAGTGTTTCCAGACTACCGGCGCTCAAAGTAAATACC                                                                              <  SRR4302535.3909972/94‑1 (MQ=60)

             GATTTCCACGGCTATGCACGTTCCGGTATTGGTTGGACAGGTAGCGGCGGTGAACAACAGTGTTTCCAGACTACCGGCGCTCAAAGTAAATACC                                                                              <  SRR4302535.1686591/94‑1 (MQ=60)

             GATTTCCACGGCTATGCACGTGCCGGTATTGGTTGGACAGGTAGCGGCGGTGAACAACAGTGTTTCCAGACTACCGGCGCTCAAAGTAAATACC                                                                              <  SRR4302535.2711605/94‑1 (MQ=60)

              ATTTCCACGGCTATGCACGTTTCGGTATTTGTTTGACAGGTAGCGGCGGTGAACAACAGTGTTTCCAGACTACCGGCGCTCAAAGTAAATACCG                                                                             <  SRR4302535.4012416/94‑1 (MQ=60)

              ATTTCCACGGCTATGCACGTTCCGGTATTGGTTGGACAGGTAGCGGCGGTGAACAACAGTGTTTCCAGACTACCGGCGCTCAAAGTAAATACCG                                                                             <  SRR4302535.2983262/94‑1 (MQ=60)

              AGTTCCACGGCTATGCACGTTCCGGTATTGGTTGGACAGGTAGCGGCGGTGAACAACAGTGTTTCCAGACTACCGGCGCTCAAAGTAAATACCG                                                                             <  SRR4302535.1121146/94‑1 (MQ=60)

                  CCACGGCTATGCACGTTCCGGTATTGGTTGGACAGGTAGCGGCGGTGAACAACAGTGTTTCCAGACTACCGGCGCTCAAAGTAAATACCGTCTT                                                                         <  SRR4302535.3098866/94‑1 (MQ=60)

                   CACGGCTATGCACGTTCCGGTATTGGTTGGACAGGTAGCGGCGGTGAACAACAGTGTTTCCAGACTACCGGCGCTCAAAGTAAATACCGTCTTG                                                                        >  SRR4302535.2262016/1‑94 (MQ=60)

                   CACGGCTATGCACGTTCCGGTATTGGTTGGACAGGTAGCGGCGGTGAACAACAGTGTTTCCAGACTACCGGCGCTCAAAGTAAATACCGTCTTG                                                                        <  SRR4302535.3376644/94‑1 (MQ=60)

                     CGGCTATGCACGTTCCGGTATTGGTTGGACAGGTAGCGGCGGTGAACAACAGTGTTTCCAGACTACCGGCGCTCAAAGTAAATACCGTCTTGGC                                                                      >  SRR4302535.354123/1‑94 (MQ=60)

                        CTATGCACGTTCCGGTATTGGTTGGACAGGTAGCGGCGGTGAACAACAGTGTTTCCAGACTACCGGCGCTCAAAGTAAATACCGTCTTGGCCAC                                                                   >  SRR4302535.2455209/1‑94 (MQ=60)

                        CTATGCACGTTCCGGTATTGGTTGGACAGGTAGCGGCGGTGAACAACAGTGTTTCCAGACTACCGGCGCTCAAAGTAAATACCGTCTTGGCAAC                                                                   >  SRR4302535.540275/1‑94 (MQ=60)

                        CTATGCACGTTCCGGTATTGGTTGGACAGGTAGCGGCGGTGAACAACAGTGTTTCCAGACTAACGGCGCTCAAAGTAAATACCGTCTTGGCAAC                                                                   >  SRR4302535.1225404/1‑94 (MQ=60)

                             CACGTTCCGGTATTGGTTGGACAGGTAGCGGCGGTGAACAACAGTGTTTCCAGACTACCGGCGCTCAAAGTAAATACCGTCTTGGCAACGAATG                                                              <  SRR4302535.3337551/94‑1 (MQ=60)

                             CACGTTCCGGTATTGGTTGGACAGGTAGCGGCGGTGAACAACAGTGTTTCCAGACTACCGGCGCTCAAAGTAAATACCGTCTTGGCAACGAATG                                                              <  SRR4302535.1423664/94‑1 (MQ=60)

                              ACGTTCCGGTATTGGTTGGACAGGTAGCGGCGGTGAACAACAGTGTTTCCAGACTACCGGCGCTCAAAGTAAATACCGTCTTGGCAACGAATGT                                                             <  SRR4302535.1961104/94‑1 (MQ=60)

                              ACGTTCCGGTATTGGTTGGACAGGTAGCGGCGGTGAACAACAGTGTTTCCAGACTACCGGCGCTCAAAGTAAATACCGTCTTGGCAACGAATGT                                                             <  SRR4302535.4370736/94‑1 (MQ=60)

                               CGTTCCGGTATTGGTTGGACAGGTAGCGGCGGTGAACAACAGAGTTTCCAGACTACCGGCGCTCAAAGTAAATACCGTCTTCGCAACGAATGTG                                                            >  SRR4302535.1240023/1‑94 (MQ=60)

                                GTTCCGGTATTGGTTGGACAGGTAGCGGCGGTGAACAACAGTGTTTCCAGACTACCGGCGCTCAAAGTAAATACCGTCTTGGCAACGAATGTGA                                                           <  SRR4302535.4491133/94‑1 (MQ=60)

                                 TTCCGGTATTGGTTGGACAGGTAGCGGCGGTGAACAACAGTGTTTCCAGACTACCGGCGCTCAAAGTAAATACCGTCTTGGCAACGAATGTGAA                                                          >  SRR4302535.4436674/1‑94 (MQ=60)

                                  TCCGGTATTGGTTGGACAGGTAGCGGCGGTGAACAACAGTGTTTCCAGACTACCGGCGCTCAAAGTAAATACCGTCTTGGCAACGAATGTGAAA                                                         <  SRR4302535.1236451/94‑1 (MQ=60)

                                   CCGGTATTGGTTGGACAGGTAGCGGCGGTGAACAACAGTGTTTCCAGACTACCGGCGCTCAAAGTAAATACCGTCTTGGCAACGAATGAGAAAC                                                        >  SRR4302535.3114269/1‑94 (MQ=60)

                                    CGGTATTGGTTGGACAGGTAGCGGCGGTGAACAACAGTGTTTCCAGACTACCGGCGCTCAAAGTAAATACCGTCTTGGCAACGAATGTGAAACT                                                       >  SRR4302535.4111298/1‑94 (MQ=60)

                                    CGGTATTGGTTGGACAGGTAGCGGCGGTGAACAACAGTGTTTCCAGACTACCGGCGCTCAAAGTAAATACCGTCTTGGCAACGAATGTGAAACT                                                       <  SRR4302535.4073850/94‑1 (MQ=60)

                                       TATTGGTTGGACAGGTAGCGGCGGTGAACAACAGTGTTTCCAGACTACCGGCGCTCAAAGTAAATACCGTCTTGGCAACGAATGTGAAACTTAT                                                    >  SRR4302535.3567069/1‑94 (MQ=60)

                                       TATTGGTTGGACAGGTAGCGGCGGTGAACAACAGTGTTTCCAGACTACCGGCGCTCAAAGTAAATACCGTCTTGGCAACGAATGTGAAACTTAT                                                    <  SRR4302535.773905/94‑1 (MQ=60)

                                       TATTGGTTGGACAGGTAGCGGCGGTGAACAACAGTGTTTCCAGACTACCGGCGCTCAAAGTAAATACCGTCTTGGCAACGAATGTGAAACTTAT                                                    <  SRR4302535.1502439/94‑1 (MQ=60)

                                        ATTGGTTGGACAGGTAGCGGCGGTGAACAACAGTGTTTCCAGACTACCGGCGCTCAAAGTAAATACCGTCTTGGCAACGAATGTGAAACTTATG                                                   <  SRR4302535.1065455/94‑1 (MQ=60)

                                          TGGTTGGACAGGTAGCGGCGGTGAACAACAGTGTTTCCAGACTACCGGCGCTCAAAGTAAATACCGTCTTGGCAACGAATGTGAAACTTATGCT                                                 >  SRR4302535.529424/1‑94 (MQ=60)

                                                  CAGGTAGCGGCGGTGAACAACAGTGTTTCCAGACTACCGGCGCTCAAAGTAAATACCGTCTTGGCAACGAATGTGAAACTTATGCTGAATTAAA                                         <  SRR4302535.17776/94‑1 (MQ=60)

                                                    GGTAGCGGCGGTGAACAACAGTGTTTCCAGACTACCGGCGCTCAAAGTAAATACCGTCTTGGCAACGAATGTGAAACTTATGCTGAATTAAAAT                                       >  SRR4302535.61098/1‑94 (MQ=60)

                                                    GGTAGCGGCGGTGAACAACAGTGTTTCCAGACTACCGGCGCTCAAAGTAAATACCGTCTTGGCAACGAATGTGAAACTTATGCTGAATTAAAAT                                       >  SRR4302535.3596042/1‑94 (MQ=60)

                                                      TAGCGGCGGTGAACAACAGTGTTTCCAGACTACCGGCGCTCAAAGTAAATACCGTCTTGGCAACGAATGTGAAACTTATGCTGAATTAAAATTG                                     <  SRR4302535.12489/94‑1 (MQ=60)

                                                      TAGCGGCGGTGAACAACAGTGTTTCCAGACTACCGGCGCTCAAAGTAAATACCGTCTTGGCAACGAATGTGAAACTTATGCTGAATTAAAATTG                                     <  SRR4302535.4691563/94‑1 (MQ=60)

                                                      TAGCGGCGGTGAACAACAGTGTTTCCAGACTACCGGCGCTCAAAGTAAATACCGTCTTGGCAACGAATGTGAAACTTATGCTGAATTAAAATTG                                     <  SRR4302535.2090250/94‑1 (MQ=60)

                                                      TAGCGGCGGTGAACAACAGTGTTTCCAGACTACCGGCGCTCAAAGTAAATACCGTCTTGGCAACGAATGTGAAACTTATGCTGAATTAAAATTG                                     <  SRR4302535.1776305/94‑1 (MQ=60)

                                                       AGCGGCGGTGAACAACAGTGTTTCCAGACTACCGGCGCTCAAAGTAAATACCGTCTTGGCAACGAATGTGAAACTTATGCTGAATTAAAATTGG                                    <  SRR4302535.2874125/94‑1 (MQ=60)

                                                        GCGGCGGTGAACAACAGTGTTTCCAGACTACCGGCGCTCAAAGTAAATAACGTCTTGGCAACGAATGTGAAACTTATGCTGAATTAAAATTGGG                                   >  SRR4302535.3818898/1‑94 (MQ=60)

                                                         CGGCGGTGAACAACAGTGTTTCCAGACTACCGGCGCTCAAAGTAAATACCGTCTTGGCAACGAATGTGAAACTTATGCTGAATTAAAATTGGGT                                  <  SRR4302535.3476419/94‑1 (MQ=60)

                                                          GGCGGTGAACAACAGTGTTTCCAGACTACCGGCGCTCAAAGTAAATACCGTCTTGGCAACGAATGTGAAACTTATGCTGAATTAAAATTGGGTC                                 >  SRR4302535.4524041/1‑94 (MQ=60)

                                                           GCGGTGAACAACAGTGTTTCCAGACTACCGGCGCTCAAAGTAAATACCGTCTTGGCAACGAATGTGAAACTTATGCTGAATTAAAATTGGGTCA                                <  SRR4302535.179966/94‑1 (MQ=60)

                                                            CGGTGAACAACAGTGTTTCCAGACTACCGGCGCTCAAAGTAAATACCGTCTTGGCAACGAATGTGAAACTTATGCTGAATTAAAATTGGGTCAG                               >  SRR4302535.1619003/1‑94 (MQ=60)

                                                             GGTGAACAACAGTGTTTCCAGACTACCGGCGCTCAAAGTAAATACCGTCTTGGCAACGAATGTGAAACTTATGCTGAATTAAAATTGGGTCAGG                              >  SRR4302535.1958985/1‑94 (MQ=60)

                                                             GGTGAACAACAGTGTTTCCAGACTACCGGCGCTCAAAGTAAATACCGTCTTGGCAACGAATGTGAAACTTATGCTGAATTAAAATTGGGTCAGG                              <  SRR4302535.1992263/94‑1 (MQ=60)

                                                             GGTGAACAACAGTGTTTCCAGACTACCGGCGCTCAAAGTAAATACCGTCTTGGCAACGAATGTGAAACTTATGCTGAATTAAAATTGGGTCAGG                              >  SRR4302535.2036386/1‑94 (MQ=60)

                                                             GGTGAACAACAGTGTTTCCAGACTACCGGCGCTCAAAGTAAATACCGTCTTGGCAACGAATGTGAAACTTATGCTGAATTAAAATTGGGTCAGG                              >  SRR4302535.4364158/1‑94 (MQ=60)

                                                             GGTGAACAACAGTGTTTCCAGACTACCGGCGCTCAAAGTAAATACCGTCTTGGCAACGAATGTGAAACTTATGCTGAATTAAAATTGGGTCAGG                              <  SRR4302535.3871253/94‑1 (MQ=60)

                                                              GTGAACAACAGTGTTTCCAGACTACCGGCGCTCAAAGTAAATACCGTCTTGGCAACGAATGTGAAACTTATGCTGAATTAAAATTGGGTCAGGA                             <  SRR4302535.4046088/94‑1 (MQ=60)

                                                               gtAACAACCGTGTTTGCAAACTACCGGCGCTCAAAGTAAATACCGTCTTGGCAACGAATGTGAAACTTATGCTGAATTAAAATTGGGTCAGGAA                            <  SRR4302535.2464184/92‑1 (MQ=60)

                                                               gcgtggtCAGTGTTTCCAGACTACCGGCGCTCAAAGTAAATACCGTCTTGGCAACGAATGTGAAACTTATGCTGAATTAAAATTGGGTCAGGAA                            <  SRR4302535.4666560/87‑1 (MQ=60)

                                                                  ACAGCAGTGTTTCCAGACTACCGGTGCTCAAAGTAAATACCGTCTTGGCAACGAATGTGAAACTTATGCTGAATTAAAATTGGGTCAGGAAGTG                         <  SRR4302535.1182825/94‑1 (MQ=60)

                                                                  ACAACAGTGTTTCCAGACTACCGGCGCTCAAAGTAAATACCGTCTTGGCAACGAATGTGAAACTTATGCTGAATTAAAATTGGGTCAGGAAGTG                         <  SRR4302535.844530/94‑1 (MQ=60)

                                                                  ACAACAGTGTTTCCAGACTACCGGCGCTCAAAGTAAATACCGTCTTGGCAACGAATGTGAAACTTATGCTGAATTAAAATTGGGTCAGGAAGTG                         >  SRR4302535.688421/1‑94 (MQ=60)

                                                                  ACAACAGTGTTTCCAGACTACCGGCGCTCAAAGTAAATACCGTCTTGGCAACGAATGTGAAACTTATGCTGAATTAAAATTGGGTCAGGAAGTG                         <  SRR4302535.3114086/94‑1 (MQ=60)

                                                                    AACAGTGTTTCCAGACTACCGGCGCTCAAAGTAAATACCGTCTTGGCAACGAATGTGAAACTTATGCTGAATTAAAATTGGGTCAGGAAGTGTG                       >  SRR4302535.1765832/1‑94 (MQ=60)

                                                                     ACAGTGTTTCCAGACTACCGGCGCTCAAAGTAAATACCGTCTTGGCAACGAATGTGAAACTTATGCTGAATTAAAATTGGGTCAGGAAGTGTGG                      <  SRR4302535.4669944/94‑1 (MQ=60)

                                                                     ACAGTGTTTCCAGACTACCGGCGCTCAAAGTAAATACCGTCTTGGCAACGAATGTGAAACTTATGCTGAATTAAAATTGGGTCAGGAAGTGTGG                      <  SRR4302535.4613293/94‑1 (MQ=60)

                                                                       AGTGTTTCCAGACTACCGGCGCTCAAAGTAAATACCGTCTTGGCAACGAATGTGAAACTTATGCTGAATTAAAATTGGGTCAGGAAGTGTGGAA                    >  SRR4302535.3540794/1‑94 (MQ=60)

                                                                       AGTGTTTCCAGACTACCGGCGCTCAAAGTAAATACCGTCTTGGCAACGAATGTGAAACTTATGCTGAATTAAAATTGGGTCAGGAAGTGTGGAA                    <  SRR4302535.745679/94‑1 (MQ=60)

                                                                         TGTTTCCAGACTACCGGCGCTCAAAGTAAATACCGTCTTGGCAACGAATGTGAAACTTATGCTGAATTAAAATTGGGTCAGGAAGTGTGGAAAG                  >  SRR4302535.4248936/1‑94 (MQ=60)

                                                                          GTTTCCAGACTACCGGCGCTCAAAGTAAATACCGTCTTGGCAACGAATGTGAAACTTATGCTGAATTAAAATTGGGTCAGGAAGTGTGGAAAGA                 >  SRR4302535.2482468/1‑94 (MQ=60)

                                                                           TTTCCAGACTACCGGCGCTCAAAGTAAATACCGTCTTGGCAACGAATGTGAAACTTATGCTGAATTAAAATTGGGTCAGGAAGTGTGGAAAGAG                <  SRR4302535.4488140/94‑1 (MQ=60)

                                                                            TTCCAGACTACCGGCGCTCAAAGTAAATACCGTCTTGGCAACGAATGTGAAACTTATGCTGAATTAAAATTGGGTCAGGAAGTGTGGAAAGAGG               <  SRR4302535.3717000/94‑1 (MQ=60)

                                                                            TTCCAGACTACCGGCGCTCAAAGTAAATACCGTCTTGGCAACGAATGTGAAACTTATGCTGAATTAAAATTGGGTCAGGAAGTGTGGAAAGAGG               <  SRR4302535.3251767/94‑1 (MQ=60)

                                                                             TCCAGACTACCGGCGCTCAAAGTAAATACCGTCTTGGCAACGAATGTGAAACTTATGCTGAATTAAAATTGGGTCAGGAAGTGTGGAAAGAGGG              <  SRR4302535.3931221/94‑1 (MQ=60)

                                                                             TCCAGACTACCGGCGCTCAAAGTAAATACCGTCTTGGCAACGAATGTGAAACTTATGCTGAATTAAAATTGGGTCAGGAAGTGTGGAAAGAGGG              <  SRR4302535.1197797/94‑1 (MQ=60)

                                                                              CCAGACTACCGGCGCTCAAAGTAAATACCGTCTTGGCAACGAATGTGAAACTTATGCTGAATTAAAATTGGGTCAGGAAGTGTGGAAAGAGGGC             >  SRR4302535.3624176/1‑94 (MQ=60)

                                                                              CCAGACTACCGGCGCTCAAAGTAAATACCGTCTTGGCAACGAATGTGAAACTTATGCTGAATTAAAATTGGGTCAGGAAGTGTGGAAAGAGGGC             <  SRR4302535.3442876/94‑1 (MQ=60)

                                                                               CAGACTACCGGCGCTCAAAGTAAATACCGTCTTGGCAACGAATGTGAAACTTATGCTGAATTACAATTGGGTCAGGAAGTGTGGAAAGA‑GGCGA           >  SRR4302535.3274021/1‑94 (MQ=60)

                                                                               CAGACTACCGGCGCTCAAAGTAAATACCGTCTTGGCAACGAATGTGAAACTTATGCTGAATTAAAATTGGGTCAGGAAGTGTGGAAAGAGGGCG            <  SRR4302535.1028687/94‑1 (MQ=60)

                                                                               CAGACTACCGGCGCTCAAAGTAAATACCGTCTTGGCAACGAATGTGAAACTTATGCTGAATTAAAATTGGGTCAGGAAGTGTGGAAAGAGGGCG            >  SRR4302535.2851731/1‑94 (MQ=60)

                                                                               CAGACTACCGGCGCTCAAAGTAAATACCGTCTTGGCAACGAATATGAAACTTATGCTGAATTAAAATTGGGTCAGGAAGTGTGGAAAGAGGGCG            >  SRR4302535.2536194/1‑94 (MQ=60)

                                                                                 GACTACCGGCGCTCAAAGTAAATACCGTCTTGGCAACGAATGTGAAACTTATGCTGAATTAAAATTGGGTCAGGAAGTGTGGAAAGAGGGCGAT          <  SRR4302535.1148026/94‑1 (MQ=60)

                                                                                  ACTACCGGCGCTCAAAGTAAATACCGTCTTGGCAACGAATGTGAAACTTATGCTGAATTAAAATTGGGTCAGGAAGTGTGGAAAGAGGGCGATA         >  SRR4302535.692139/1‑94 (MQ=60)

                                                                                  ACTACCGGCGCTCAAAGTAAATACCGTCTTGGCAACGAATGTGAAACTTATGCTGAATTAAAATTGGGTCAGGAAGTGTGGAAAGAGGGCGATA         >  SRR4302535.2968594/1‑94 (MQ=60)

                                                                                   CTACCGGCGCTCAAAGTAAATACCGTCTTGGCAACGAATGTGAAACTTATGCTGAATTAAAATTGGGTCAGGAAGTGTGGAAAGAGGGCGATAA        <  SRR4302535.230955/94‑1 (MQ=60)

                                                                                    TACCGGCGCTCAAAGTAAATACCGTCTTGGCAACGAATGTGAAACTTATGCTGAATTAAAATTGGGTCAGGAAGTGTGGAAAGAGGGCGATAAG       >  SRR4302535.1288313/1‑94 (MQ=60)

                                                                                     ACCGGCGCTCAAAGTAAATACCGTCTTGGCAACGAATGTGAAACTTATGCTGAATTAAAATTGGGTCAGGAAGTGTGGAAAGAGGGCGATAAGA      >  SRR4302535.3493308/1‑94 (MQ=60)

                                                                                     ACCGGCGCTCAAAGTAAATACCGTCTTGGCAACGAATGTGAAACTTATGCTGAATTAAAATTGGGTCAGGAAGTGTGGAAAGAGGGCGATAAGA      <  SRR4302535.1184486/94‑1 (MQ=60)

                                                                                      CCGGCGCTCAAAGTAAATACCGTCTTGGCAACGAATGTGAAACTTATGCTGAATTAAAATTGGGTCAGGAAGTGTGGAAAGAGGGCGATAAGAG     >  SRR4302535.3980782/1‑94 (MQ=60)

                                                                                       CGGCGCTCAAAGTAAATACCGTCTTGGCAACGAATGTGAAACTTATGCTGAATTAAAATTGGGTCAGGAAGTGTGGAAAGAGGGCGATAAGAGC    <  SRR4302535.4598384/94‑1 (MQ=60)

                                                                                        GGCGCTCAAAGTAAATACCGTCTTGGCAACGAATGTGAAACTTATGCTGAATTAAAATTGGGTCAGGAAGTGTGGAAAGAGGGCGATAAGAGCT   <  SRR4302535.2679272/94‑1 (MQ=60)

                                                                                         GCGCTCAAAGTAAATACCGTCTTGGCAACGAATGTGAAACTTATGCTGAATTAAAATTGGGTCAGGAAGTGTGGAAAGAGGGCGATAAGAGCTT  <  SRR4302535.3658700/94‑1 (MQ=60)

GGCAATGGCTGTTGATTTCCACGGCTATGCACGTTCCGGTATTGGTTGGACAGGTAGCGGCGGTGAACAACAGTGTTTCCAGACTACCGGTGCTCAAAGTAAATACCGTCTTGGCAACGAATGTGAAACTTATGCTGAATTAAAATTGGGTCAGGAAGTGTGGAAAGAGGGCGATAAGAGCTT  >  NC_000913/4245615‑4245797

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 7 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`