Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1873 183.1 9376148 98.2% 9207377 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,246,630 A→G T361A (ACC→GCC)  lamB → phage lambda receptor protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,246,6300AG98.2% 674.8 / ‑0.3 223T361A (ACC→GCC) lamBphage lambda receptor protein
Reads supporting (aligned to +/- strand):  ref base A (2/2);  new base G (96/123);  total (98/125)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.54e-01

Only 100 of 236 total aligned reads displayed.

TGGACCGTCGGTATTCGCCCGATGTACAAGTGGACGCCAATCATGAGCACCGTGATGGAAATCGGCTACGACAACGTCGAATCCCAGCGCACCGGCGACAAGAACAATCAGTACAAAATTACCCTCGCACAACAATGGCAGGCTGGCGACAGCATCTGGTCACGCCCGGCTATTCGTGTC  >  NC_000913/4246540‑4246719
                                                                                          |                                                                                         
tggACCGTCGGTATTCGCCCGATGTACAAGTGGACGCCAATCATGAGCACCGTGATGGAAATCGGCTACGACAACGTCGAATCCCAGCGCGcc                                                                                         >  1:1177892/1‑93 (MQ=255)
  gACCGTCGGTATTCGCCCGATGTACAAGTGGACGCCAATCATGAGCACCGTGATGGAAATCGGCTACGACAACGTCGAATCCCAGCGCGCCgg                                                                                       >  2:2343487/1‑93 (MQ=255)
     cGTCGGTATTCGCCCGATGTACAAGTGGACGCCAATCATGAGCACCGTGATGGAAATCGGCTACGACAACGTCGAATCCCAGCGCGCCGGCGa                                                                                    <  1:1059715/93‑1 (MQ=255)
     cGTCGGTATTCGCCCGATGTACAAGTGGACGCCAATCATGAGCACCGTGATGGAAATCGGCTACGACAACGTCGAATCCCAGCGCGCCGGCGa                                                                                    >  2:3142991/1‑93 (MQ=255)
      gTCGGTATTCGCCCTATGTACAAGTGGACGCCAATCATGAGCGCGGTGATGGAAATCGGCTACGACAACGTCGAATCCCAGCGCGCCGGCGAc                                                                                   <  2:3373180/93‑1 (MQ=255)
      gTCGGTATTCGCCCGATGTACAAGTGGACGCCAATCATGAGCACCGTGATGGAAATCGGCTACGACAACGTCGAATCCCAGCGCGCCGGCGAc                                                                                   >  2:816497/1‑93 (MQ=255)
       tCGGTATTCGCCCGATGTACAAGTGGACGCCAATCATGAGCACCGTGATGGAAATCGGCTACGACAACGTCGAATCCCAGCGCGCCGGCGACa                                                                                  >  1:2792986/1‑93 (MQ=255)
       tCGGTATTCGCCCGATGTACAAGTGGACGCCAATCATGAGCACCGTGATGGAAATCGGCTACGACAACGTCGAATCCCAGCGCGCCGGCGACa                                                                                  >  1:294712/1‑93 (MQ=255)
       tCGGTATTCGCCCGATGTACAAGTGGACGCCAATCATGAGCACCGTGATGGAAATCGGCTACGACAACGTCGAATCCCAGCGCGCCGGCGACa                                                                                  <  2:3208163/93‑1 (MQ=255)
       tCGGTATTCGCCCGATGTACAAGTGGACGCCAATCATGAGCACCGTGATGGAAATCGGCTACGACAACGTCGAATCCCAGCGCGCCGGCGACa                                                                                  >  2:3732700/1‑93 (MQ=255)
       tCGGTATTCGCCCGATGTACAAGTGGACGCCAATCATGAGCACCGTGATGGAAATCGGCTACGACAACGTCGAATCCCAGCGCGCCGGCGACa                                                                                  <  2:4548680/93‑1 (MQ=255)
        cGGTATTCGCCCGATGTACAAGTGGACGCCAATCATGAGCACCGTGATGGAAATCGGCTACGACAACGTCGAATCCCAGCGCGCCGGCGACaa                                                                                 <  1:1716507/93‑1 (MQ=255)
          gTATTCGCCCGATGTACAAGTGGACGCCAATCATGAGCACCGTGATGGAAATCGGCTACGACAACGTCGAATCCCAGCGCGCCGGCGACAAGa                                                                               <  2:1685333/93‑1 (MQ=255)
           tATTCGCCCGATGTACAAGTGGACGCCAATCATGAGCACCGTGATGGAAATCGGCTACGACAACGTCGAATCCCAGCGCGCCGGCGACAAGaa                                                                              >  1:1938456/1‑93 (MQ=255)
           tATTCGCCCGATGTACAAGTGGACGCCAATCATGAGCACCGTGATGGAAATCGGCTACGACAACGTCGAATCCCAGCGCGCCGGCGACAAGaa                                                                              >  2:1645889/1‑93 (MQ=255)
           tATTCGCCCGATGTACAAGTGGACGCCAATCATGAGCACCGTGATGGAAATCGGCTACGACAACGTCGAATCCCAGCGCGCCGGCGACAAGaa                                                                              >  2:4109049/1‑93 (MQ=255)
            aTTCGCCCGATGTACAAGTGGACGCCAATCATGAGCACCGTGATGGAAATCGGCTACGACAACGTCGAATCCCAGCGCGCCGGCGACAAGAAc                                                                             >  2:1155291/1‑93 (MQ=255)
            aTTCGCCCGATGTACAAGTGGACGCCAATCATGAGCACCGTGATGGAAATCGGCTACGACAACGTCGAATCCCAGCGCGCCGGCGACAAGAAc                                                                             >  1:4408852/1‑93 (MQ=255)
            aTTCGCCCGATGTACAAGTGGACGCCAATCATGAGCACCGTGATGGAAATCGGCTACGACAACGTCGAATCCCAGCGCGCCGGCGACAAGAAc                                                                             >  1:3650222/1‑93 (MQ=255)
              tCGCCCGATGTACAAGTGGACGCCAATCATGAGCACCGTGATGGAAATCGGCTACGACAACGTCGAATCCCAGCGCGCCGGCGACAAGAACaa                                                                           <  1:4601339/93‑1 (MQ=255)
               cGCCCGATGTACAAGTGGACGCCAATCATGAGCACCGTGATGGAAATCGGCTACGACAACGTCGAATCCCAGCGCGCCGGCGACAAGAACAAt                                                                          <  2:1202825/93‑1 (MQ=255)
               cGCCCGATGTACAAGTGGACGCCAATCATGAGCACCGTGATGGAAATCGGCTACGACAACGTCGAATCCCAGCGCGCCGGCGACAAGAACAAt                                                                          >  1:2269242/1‑93 (MQ=255)
                gCCCGATGTACAAGTGGACGCCAATCATGAGCACCGTGATGGAAATCGGCTACGACAACGTCGAATCCCAGAGCGCCGGCGACAAGAACAATc                                                                         >  2:2797914/1‑93 (MQ=255)
                 cccGATGTACAAGTGGACGCCAATCATGAGCACCGTGATGGAAATCGGCTACGACAACGTCGAATCCCAGCGCGCCGGCGACAAGAACAATCa                                                                        <  2:2903281/93‑1 (MQ=255)
                   cGATGTACAAGTGGACGCCAATCATGAGCACCGTGATGGAAATCGGCTACGACAACGTCGAATCCCAGCGCGCCGGCGACAAGAACAATCAGt                                                                      <  2:3740730/93‑1 (MQ=255)
                   cGATGTACAAGTGGACGCAAATCATGAGCACCGTGATGGAAATCGGCTACGACAACGTCGAATCCCAGCGCGCCGGCGACAAGAACAATCAGt                                                                      <  2:4294190/93‑1 (MQ=255)
                   cGATATAAAAGTGGACGCGAATCATGAGCCCCGAGATGGATATCGGCTACGCCAACGTCGAACCACAGCGCGCCGGCGACAAGAACAATCAGt                                                                      <  2:2510509/93‑1 (MQ=255)
                    gATGTACAAGTGGACGCCAATCATGAGCACCGTGATGGAAATCGGCTACGACAACGTCGAATCCCAGCGCGCCGGCGACAAGAACAATCAGTa                                                                     >  1:4377086/1‑93 (MQ=255)
                    caTGTACAAGTGGACGCGAATCATGAGCACCGTGATGGAAATCGGCTACGACAACGTCGAATCCCAGCGCGCCGGCGACAAGAACAATCAGTa                                                                     <  1:185642/92‑1 (MQ=255)
                     acgTACAAGTGGACGCCAATCATGAGCACCGTGATGGAAATCGGCTACGACAACGTCGAATCCCAGCGCGCCGGCGACAAGAACAATCAGTAc                                                                    <  1:3366148/91‑1 (MQ=255)
                      tGTACAAGTGGACGCCAATCATGAGCACCGTGATGGAAATCGGCTACGACAACGTCGAATCCCAGCGCGCCGGCGACAAGAACAATCAGTACa                                                                   <  1:2046772/93‑1 (MQ=255)
                       gTACAAGTGGACGCCAATCATGAGCACCGTGATGGAAATCGGCTACGACAACGTCGAATCCCAGCGCGCCGGCGACAAGAACAATCAGTACaa                                                                  >  1:4105989/1‑93 (MQ=255)
                        tACAAGTGGACGCCAATCATGAGCACCGTGATGGAAATCGGCTACGACAACGTCGAATCCCAGCGCGCCGGCGACAAGAACAATCAGTACaaa                                                                 >  1:2612923/1‑93 (MQ=255)
                        tACAAGTGGACGCCAATCATGAGCACCGTGATGGAAATCGGCTACGACAACGTCGAATCCCAGCGCGCCGGCGACAAGAAAAATCAGTACaaa                                                                 >  1:2165396/1‑93 (MQ=255)
                          cAAGTGGACGCCAATCATGAGCACCGTGATGGAAATCGGCTACGACAACGTCGAATCCCAGCGCGCCGGCGACAAGAACAATCAGTACAAAAt                                                               <  2:247370/93‑1 (MQ=255)
                           aaGTGGACGCCAATCATGAGCACCGTGATGGAAATCGGCTACGACAACGTCGAATCCCAGCGCGCCGGCGACAAGAACAATCAGTACAAAAtt                                                              <  1:979786/93‑1 (MQ=255)
                            aGTGGACGCCAATCATGAGCACCGTGATGGAAATCGGCTACGACAACGTCGAATCCCAGCGCGCCGGCGACAAGAACAATCAGTACAAAATTa                                                             >  2:2035120/1‑93 (MQ=255)
                            aGTGGACGCCAATCATGAGCACCGTGATGGAAATCGGCTACGACAACGTCGAATCCCAGCGCGCCGGCGACAAGAACAATCAGTACAAAATTa                                                             >  2:1930435/1‑93 (MQ=255)
                            aGTGGACGCCAATCATGAGCACCGTGATGGAAATCGGCTACGACAACGTCGAATCCCAGCGCGCCGGCGACAAGAACAATCAGTACAAAATTa                                                             >  2:2412827/1‑93 (MQ=255)
                            aGTGCACGCTAAGCATGAGCACCGTAATGGAAATCGGCTACGACAACGTCGAATCCCAGCGCGCCGGCGACAAGAACAATCAGTACAAAATTa                                                             <  2:521736/93‑1 (MQ=255)
                             gTGGACGCCAATCATGAGCACCGTGATGGAAATCGGCTACGACAACGTCGAATCCCAGCGCGCCGGCGACAAGAACAATCAGTACAAAATTAc                                                            >  2:1514410/1‑93 (MQ=255)
                             gTGGACGCCAATCATGAGCACCGTGATGGAAATCGGCTACGACAACGTCGAATCCCAGCGCGCCGGCGACAAGAACAATCAGTACAAAATTAc                                                            >  1:1808419/1‑93 (MQ=255)
                             gTGGACGCCAATCATGAGCACCGTGATGGAAATCGGCTACGACAACGTCGAATCCCAGCGCGCCGGCGACAAGAACAATCAGTACAAAATTAc                                                            >  1:2604363/1‑93 (MQ=255)
                              tGGACGCCAATCATGAGCACCGTGATGGAAATCGGCTACGACAACGTCGAATCCCAGCGCGCCGGCGACAAGAACAATCAGTACAAAATTAcc                                                           <  1:3525712/93‑1 (MQ=255)
                               ggACGCCAATCATGAGCACCGTGATGGAAATCGGCTACGACAACGTCGAATCCCAGCGCGCCGGCGACAAGAACAATCAGTACAAAATTAccc                                                          <  2:3157617/93‑1 (MQ=255)
                               ggACGCCAATCATGAGCACCGTGATGGAAATCGGCTACGACAACGTCGAATCCCAGCGCGCCGGCGACAAGAACAATCAGTACAAAATTAccc                                                          >  2:398076/1‑93 (MQ=255)
                                gACGCCAATCATGAGCACCGTGATGGAAATCGGCTACGACAACGTCGAATCCCAGCGCGCCGGCGACAAGAACAATCAGTACAAAATTACCCt                                                         <  2:2358548/93‑1 (MQ=255)
                                gACGCCAATCATGAGCACCGTGATGGAAATCGGCTACGACAACGTCGAATCCCAGCGCGCCGGCGACAAGAACAATCAGTACAAAATTACCCt                                                         <  1:728663/93‑1 (MQ=255)
                                 aCGCCAATCATGAGCACCGTGATGGAAATCGGCTACGACAACGTCGAATCCCAGCGCGCCGGCGACAAGAACAATCAGTACAAAATTACCCTc                                                        >  2:2552387/1‑93 (MQ=255)
                                 aCGCCAATCATGAGCACCGTGATGGAAATCGGCTACGACAACGTCGAATCCCAGCGCGCCGGCGACAAGAACAATCAGTACAAAATTACCCTc                                                        <  1:3758897/93‑1 (MQ=255)
                                    ccAATCATGAGCCCCGTGATGGAAATCGGCTACGACAACGTCGAATCCCAGCGCGCCGGCGACAAGAACAATCAGTACAAAAATTCCCTCGca                                                     <  2:1445758/93‑1 (MQ=255)
                                      aaTCATGAGCACCGTGATGGAAATCGGCTACGACAACGTCGAATCCCAGCGCGCCGGCGACAAGAACAATCAGTACAAAATTACCCTCGcaca                                                   >  1:1478773/1‑93 (MQ=255)
                                        tCATGAGCACCGTGATGGAAATCGGCTACGACAACGTCGAATCCCAGCGCGCCGGCGACAAGAACAATCAGTACAAAATTACCCTCGCacaaa                                                 >  1:3034447/1‑92 (MQ=255)
                                          aTGAGCACCGTGATGGAAATCGGCTACGACAACGTCGAATCCCAGCGCGCCGGCGACAAGAACAATCAGTACAAAATTACCCTCGCAcgacaa                                               >  2:1370834/1‑93 (MQ=255)
                                          aTGAGCACCGTGATGGAAATCGGCTACGACAACGTCGAATCCCAGCGCGCCGGCGACAAGAACAATCAGTACAAAATTACCCTCGCAcaacaa                                               <  1:3139161/93‑1 (MQ=255)
                                           tGAGCACCGTGATGGAAATCGGCTACGACAACGTCGAATCCCAGCGCGCCGGCGACAAGAACAATCAGTACAAAATTACCCTCGCACAACAAt                                              >  1:2767544/1‑93 (MQ=255)
                                           tGAGCACCGTGATGGAAATCGGCTACGACAACGTCGAATCCCAGCGCGCCGGCGACAAGAACAATCAGTACAAAATTACCCTCGCACAACAAt                                              <  1:1393534/93‑1 (MQ=255)
                                            gAGCACCGTGATGGAAATCGGCTACGACAACGTCGAATCCCAGCGCGCCGGCGACAAGAACAATCAGTACAAAATTACCCTCGCACAACAATg                                             <  1:3987869/93‑1 (MQ=255)
                                             aGCACCGTGATGGAAATCGGCTACGACAACGTCGAATCCCAGCGCGCCGGCGACAAGAACAATCAGTACAAAATTACCCTCGCACAACAATgg                                            <  1:1327711/93‑1 (MQ=255)
                                              gCACCGTGATGGAAATCGGCTACGACAACGTCGAATCCCAGCGCGCCGGCGACAAGAACAATCAGTACAAAATTACCCTCGCACAACAATGGc                                           >  2:1238742/1‑93 (MQ=255)
                                                aCCGTGATGGAAATCGGCTACGGCAACGTCGAATCCCAGCGCGCCGGCGACAAGAACAATCAGTACAAAATTACCCTCGCACAACAATGGCAg                                         >  2:3307661/1‑93 (MQ=255)
                                                aCCGTGATGGAAATCGGCTACGACAACGTCGAATCCCAGCGCGCCGGCGACAAGAACAATCAGTACAAAATTACCCTCGCACAACAATGGCAg                                         >  2:4240579/1‑93 (MQ=255)
                                                aCCGTGATGGAAATCGGCTACGACAACGTCGAATCCCAGCGCGCCGGCGACAAGAACAATCAGTACAAAATTACCCTCGCACAACAATGGCAg                                         <  1:472541/93‑1 (MQ=255)
                                                aCCGTGATGGAAATCGGCTACGACAACGTCGAATCCCAGCGCGCCGGCGACAAGAACAATCAGTACAAAATTACCCTCGCACAACAATGGCAg                                         <  1:3935333/93‑1 (MQ=255)
                                                aCCGTGATGGAAATCGGCTACGACAACGTCGAATCCCAGCGCGCCGGCGACAAGAACAATCAGTACAAAATTACCCTCGCACAACAATGGCAg                                         <  2:3749183/93‑1 (MQ=255)
                                                  cGTGATGGAAATCGGCTACGACAACGTCGAATCCCAGCGCGCCGGCGACAAGAACAATCAGTACAAAATTACCCTCGCACAACAATGGCAGGc                                       >  2:1597227/1‑93 (MQ=255)
                                                  cGTGATGGAAATCGGCTACGACAACGTCGAATCCCAGCGCGCCGGCGACAAGAACAATCAGTACAAAATTACCCTCGCACAACAATGGCAGGc                                       <  1:800338/93‑1 (MQ=255)
                                                   ctGATGGAAATCGGCTACGACAACGTCGAATCCCAGCGCGCCGGCGACAAGAACAATCAGTACAAAATTACCCTCGCACAACAATGGCAGGCt                                      <  2:3875748/92‑1 (MQ=255)
                                                     gATGGAAATCGGCTACGACAACGTCGAATCCCAGCGCGCCGGCGACAAGAACAATCAGTACAAAATTACCCTCGCACAACAATGGCAGGCTgg                                    <  2:3428755/93‑1 (MQ=255)
                                                      aTGGAAATCGGCTACGACAACGTCGAATCCCAGCGCGCCGGCGACAAGAACAATCAGTACAAAATTACCCTCGCACAACAATGGCAGGCTGGc                                   >  1:2584392/1‑93 (MQ=255)
                                                       tGGAAATCGGCTACGACAACGTCGAATCCCAGCGCGCCGGCGACAAGAACAATCAGTACAAAATTACCCTCGCACAACAATGGCAGGCTGGCg                                  >  1:2511035/1‑93 (MQ=255)
                                                        ggAAATCGGCTACGACAACGTCGAATCCCAGCGCGCCGGCGACAAGAACAATCAGTACAAAATTACCCTCGCACAACAATGGCAGGCTGGCGa                                 <  1:4131932/93‑1 (MQ=255)
                                                        ggAAATCGGCTACGACAACGTCGAATCCCAGCGCGCCGGCGACAAGAACAATCAGTACAAAATTACCCTCGCACAACAATGGCAGGCTGGCGa                                 <  1:2316062/93‑1 (MQ=255)
                                                          aaaTCGGCTACGACAACGTCGAATCCCAGCGCGCCGGCGACAAGAACAATCAGTACAAAATTACCCTCGCACAACAATGGCAGGCTGGCGACa                               <  2:2147528/93‑1 (MQ=255)
                                                           aaTCGGCTACGACAACGTCGAATCCCAGCGCACCGGCGACAAGAACAATCAGTACAAAATTACCCTCGCACAACAATGGCAGGCTGGCGACAg                              <  2:1477305/93‑1 (MQ=255)
                                                            aTCGTCTACGACAACGTCGAATCCCAGCGCGCCGGCGACAAGAACAATCAGTACAAAATTACCCTCGCACAACAATGGCAGGCTGGCGACAGc                             <  1:4470945/93‑1 (MQ=255)
                                                                gCTACGACACCGTCGAATCCCAGCGCGCCGGCGACAAGAACAATCAGTACAAAATTACCCTCGCACAACAATGGCAGGCTGGCGACAGCATCt                         <  2:2871441/93‑1 (MQ=255)
                                                                gCTACGACAACGTCGAATCCCAGCGCGCCGGCGACAAGAACAATCAGTACAAAATTACCCTCGCACAACAATGGCAGGCTGGCGACAGCATCt                         <  1:4224578/93‑1 (MQ=255)
                                                                gCTACGACAACGTCGAATCCCAGCGCGCCGGCGACAAGAACAATCAGTACAAAATTACCCTCGCACAACAATGGCAGGCTGGCGACAGCATCt                         <  2:610724/93‑1 (MQ=255)
                                                                gCTACGACAACGTCGAATCCCAGCGCGCCGGCGACAAGAACAATCAGTACAAAATTACCCTCGCACAACAATGGCAGGCTGGCGACAGCATCt                         >  2:4186601/1‑93 (MQ=255)
                                                                gCTACGACAACGTCGAATCCCAGCGCGCCGGCGACAAGAACAATCAGTACAAAATTACCCTCGCACAACAATGGCAGGCTGGCGACAGCATCt                         <  1:165588/93‑1 (MQ=255)
                                                                  tACGACAACGTCGAATCCCAGCGCGCCGGCGACAAGAACAATCAGTACAAAATTACCCTCGCACAACAATGGCAGGCTGGCGACAGCATCTgg                       <  1:3826396/93‑1 (MQ=255)
                                                                   aCGACAACGTCGAATCCCAGCGCGCCGGCGACAAGAACAATCAGTACAAAATTACCCTCGCACAACAATGGCAGGCTGGCGACAGCATCTGGt                      >  1:2849896/1‑93 (MQ=255)
                                                                   aCGACAACGTCGAATCCCAGCGCGCCGGCGACAAGAACAATCAGTACAAAATTACCCTCGCACAACAATGGCAGGCTGGCGACAGCATCTGGt                      <  2:3058799/93‑1 (MQ=255)
                                                                           gTCGAATCCCAGCGCGCCGGCGACAAGAACAATCAGTACAAAATTACCCTCGCACAACAATGGCAGGCTGGCGACAGCATCTGGTCACGCCCg              <  2:1078030/93‑1 (MQ=255)
                                                                           gTCGAATCCCAGCGCGCCGGCGACAAGAACAATCAGTACAAAATTACCCTCGCACAACAATGGCAGGCTGGCGACAGCATCTGGTCACGCCCg              <  1:75918/93‑1 (MQ=255)
                                                                            tCGAATCCCAGCGCGCCGGCGACAAGAACAATCAGTACAAAATTACCCTCGCACAACAATGGCAGGCTGGCGACAGCATCTGGTCACGCCCgg             >  2:793372/1‑93 (MQ=255)
                                                                             cGAATCCCAGCGCGCCGGCGACAAGAACAATCAGTACAAAATTACCCTCGCACAACAATGGCAGGCTGGCGACAGCATCTGGTCACGCCCGGc            <  1:2416326/93‑1 (MQ=255)
                                                                              gAATCCCAGCGCGCCGGCGACAAGAACAATCAGTACAAAATTACCCTCGCACAACAATGGCAGGCTGGCGACAGCATCTGGTCACGCCCGGCt           >  2:759635/1‑93 (MQ=255)
                                                                                aTCCCAGCGCGCCGGCGACAAGAACAATCAGTACAAAATTACCCTCGCACAACAATGGCAGGCTGGCGACAGCATCTGGTCACGCCCGGCTAt         >  2:3564840/1‑93 (MQ=255)
                                                                                 tCCCAGCGCGCCGGCGACAAGAACAATCAGTACAAAATTACCCTCGCACAACAATGGCAGGCTGGCGACAGCATCTGGTCACGCCCGGCTAtt        <  2:3365151/93‑1 (MQ=255)
                                                                                 tCCCAGCGCGCCGGCGACAAGAACAATCAGTACAAAATTACCCTCGCACAACAATGGCAGGCTGGCGACAGCATCTGGTCACGCCCGGCTAtt        <  1:901222/93‑1 (MQ=255)
                                                                                  cccAGCGCGCCGGCGACAAGAACAATCAGTACAAAATTACCCTCGCACAACAATGGCAGGCTGGCGACAGCATCTGGTCACGCCCGGCTATTc       <  1:369959/93‑1 (MQ=255)
                                                                                   ccAGCGCGCCGGCGACAAGAACAATCAGTACAAAATTACCCTCGCACAACAATGGCAGGCTGGCGACAGCATCTGGTCACGCCCGGCTATTCg      <  2:3543674/93‑1 (MQ=255)
                                                                                    cAGCGCGCCGGCGACAAGAACAATCAGTACAAAATTACCCTCGCACAACAATGGCAGGCTGGCGACAGCATCTGGTCACGCCCGGCTATTCgt     >  1:1136959/1‑93 (MQ=255)
                                                                                     aGCGCGCCGGCGACAAGAACAATCAGTACAAAATTACCCTCGCACAACAATGGCAGGCTGGCGACAGCATCTGGTCACGCCCGGCTATTCgtg    <  2:2262668/93‑1 (MQ=255)
                                                                                      gcgcgcCGGTGACAAGAACAATCAGTACAAAATTCCCCTCGCACAACAACGGCAGGCTGGCGACAGCATCTGGTCACGCCCGGCTATTCgtgt   <  2:2221980/93‑1 (MQ=255)
                                                                                      gcgcgcCGGCGACAAGAACAATCAGTACAAAATTACCCTCGCACAACAATGGCAGGCTGGCGACAGCATCTGGTCACGCCCGGCTATTTgtgt   >  2:2684036/1‑93 (MQ=255)
                                                                                      gcgcgcCGGCGACAAGAACAATCAGTACAAAATTACCCTCGCACAACAATGGCAGGCTGGCGACAGCATCTGGTCACGCCCGGCTATTCgtgt   <  2:4435581/93‑1 (MQ=255)
                                                                                      gcgcgcCGGCGACAAGAACAATCAGTACAAAATTACCCTCGCACAACAATGGCAGGCTGGCGACAGCATCTGGTCACGCCCGGCTATTCgtgt   <  1:997764/93‑1 (MQ=255)
                                                                                          |                                                                                         
TGGACCGTCGGTATTCGCCCGATGTACAAGTGGACGCCAATCATGAGCACCGTGATGGAAATCGGCTACGACAACGTCGAATCCCAGCGCACCGGCGACAAGAACAATCAGTACAAAATTACCCTCGCACAACAATGGCAGGCTGGCGACAGCATCTGGTCACGCCCGGCTATTCGTGTC  >  NC_000913/4246540‑4246719

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TGGTGGACCGTCGGTATTCGCCCGATGTACAAGTGGACGCCAATCATGAGCACCGTGATGGAAATCGGCTACGACAACGTCGAATCCCAGCGCACCGGCGACAAGAACAATCAGTACAAAATTACCCTCGCACAACAATGGCAGGCTGGCGACAGCATCTGGTCACGCCCGGCTATTCGTGTCTTCG  >  NC_000913/4246537‑4246723
                                                                                             |                                                                                             
TGGTGGACCGTCGGTATTCGCCCGATGTACAAGTGGACGCCAATCATGAGCACCGTGATGGAAATCGGCTACGACAACGTCGAATCCCAGCGCG                                                                                               >  SRR4302535.1382565/1‑94 (MQ=60)
TGGTGGACCGTCGGTATTCGCCCGATGTACAAGTGGACGCCAATCATGAGCACCGTGATGGAAATCGGCTACGACAACGTCGAATCCCAGCGCG                                                                                               <  SRR4302535.1550078/94‑1 (MQ=60)
TGGTGGACCGTCGGTATTCGCCCGATGTACAAGTGGACGCCAATCATGAGCACCGTGATGGAAATCGGCTACGACAACGTCGAATCCCAGCGCG                                                                                               >  SRR4302535.2808376/1‑94 (MQ=60)
  GTGGACCGTCGGTATTCGCCCGATGTACAAGTGGACGCCAATCATGAGCACCGTGATGGAAATCGGCTACGACAACGTCGAATCCCAGCGCGCC                                                                                             >  SRR4302535.4481312/1‑94 (MQ=60)
  GTGGACCGTCGGTATTCGCCCGATGTACAAGTGGACGCCAATCATGAGCACCGTGATGGAAATCGGCTACGACAACGTCGAATCCCAGCGCGCC                                                                                             >  SRR4302535.1186295/1‑94 (MQ=60)
  GTGGACCGTCGGTATTCGCCCGATGTACAAGTGGACGCCAATCATGAGCACCGTGATGGAAATCGGCTACGACAACGTCGAATCCCAGCGCGCC                                                                                             >  SRR4302535.3286080/1‑94 (MQ=60)
   TGGACCGTCGGTATTCGCCCGATGTACAAGTGGACGCCAATCATGAGCACCGTGATGGAAATCGGCTACGACAACGTCGAATCCCAGCGCGCCG                                                                                            >  SRR4302535.1104699/1‑94 (MQ=60)
   TGGACCGTCGGTATTCGCCCGATGTACAAGTGGACGCCAATCATGAGCACCGTGATGGAAATCGGCTACGACAACGTCGAATCCCAGCGCGCCG                                                                                            <  SRR4302535.428802/94‑1 (MQ=60)
    GGACCGTCGGTATTCGCCCGATGCACAAGTGGACGCCAATCATCAGCACCGAGATGGAAATCGGCTACGACAACGTCGAATCCCAGCGCGCCGG                                                                                           >  SRR4302535.741279/1‑94 (MQ=60)
      ACCGTCGGTATTCGCCCGATGTACAAGTGGACGCCAATCATGAGCACCGTGATGGAAATCGGCTACGACAACGTCGAATCCCAGCGCGCCGGCG                                                                                         <  SRR4302535.819751/94‑1 (MQ=60)
        CGTCGGTATTCGCCCGATGTACAAGTGGACGCCAATCATGAGCACCGTGATGGAAATCGGCTACGACAACGTCGAATCCCAGCGCGCCGGCGAC                                                                                       <  SRR4302535.1067261/94‑1 (MQ=60)
        CGTCGGTATTCGCCCGATGTACAAGTGGACGCCAATCATGAGCACCGTGATGGAAATCGGCTACGACAACGTCGAATCCCAGCGCGCCGGCGAC                                                                                       <  SRR4302535.1493073/94‑1 (MQ=60)
        CGTCGGTATTCGCCCGATGTACAAGTGGACGCCAATCATGAGCACCGTGATGGAAATCGGCTACGACAACGTCGAATCCCAGCGCGCCGACGAC                                                                                       >  SRR4302535.1178995/1‑94 (MQ=60)
         GTCGGTATTCGCCCGATGTACAAGTGGACGCCAATCATGAGCACCGTGATGGAAATCGGCTACGACAACGTCGAATCCCAGCGCGCCGGCGACA                                                                                      >  SRR4302535.296768/1‑94 (MQ=60)
         GTCGGTATTCGCCCGATGTACAAGTGGACGCCAATCATGAGCACCGTGATGGAAATCGGCTACGACAACGTCGAATCCCAGCGCGCCGGCGACA                                                                                      >  SRR4302535.2812862/1‑94 (MQ=60)
           CGGTATTCGCCCGATGTACAAGTGGACGCCAATCATGAGCACCGTGATGGAAATCGGCTACGACAACGTCGAATCCCAGCGCGCCGGCGACAAG                                                                                    <  SRR4302535.186919/94‑1 (MQ=60)
           CGGTATTCGCCCGATGTACAAGTGGACGCCAATCATGAGCACCGTGATGGAAATCGGCTACGACAACGTCGAATCCCAGCGCGCCGGCGACAAG                                                                                    <  SRR4302535.3942032/94‑1 (MQ=60)
           CGGTATTCGCCCGATGTACAAGTGGACGCCAATCATGAGCACCGTGATGGAAATCGGCTACGACAACGTCGAATCCCAGCGCGCCGGCGACAAG                                                                                    <  SRR4302535.1728925/94‑1 (MQ=60)
             GTATTCGCCCGATGTACAAGTGGACGCCAATCATGAGCACCGTGATGGAAATCGGCTACGACAACGTCGAATCCCAGCGCGCCGGCGACAAGAA                                                                                  >  SRR4302535.1952413/1‑94 (MQ=60)
              TATTCGCCCGATGTACAAGTGGACGCCAATCATGAGCACCGTGATGGAAATCGGCTACGACAACGTCGAATCCCAGCGCGCCGGCGACAAGAAC                                                                                 <  SRR4302535.2916997/94‑1 (MQ=60)
              TATTCGCCCGATGTACAAGTGGACGCCAATCATGAGCACCGTGATGGAAATCGGCTACGACAACGTCGAATCCCAGCGCGCCGGCGACAAGAAC                                                                                 >  SRR4302535.2286541/1‑94 (MQ=60)
              TATTCGCCCGATGTACAAGTGGACGCCAATCATGAGCACCGTGATGGAAATCGGCTACGACAACGTCGAATCCCAGCGCGCCGGCGACAAGAAC                                                                                 >  SRR4302535.3675497/1‑94 (MQ=60)
              TATTCGCCCGATGTACAAGTGGACGCCAATCATGAGCACCGTGATGGAAATCGGCTACGACAACGTCGAATCCCAGCGCGCCGGCGACAAGAAC                                                                                 >  SRR4302535.872629/1‑94 (MQ=60)
              TATTCGCCCGATGTACAAGTGGACGCCAATCATGAGCACCGTGATGGAAATCGGCTACGACAACGTCGAATCCCAGCGCGCCGGCGACAAGAAC                                                                                 >  SRR4302535.4439023/1‑94 (MQ=60)
                 TCGCCCGATGTACAAGTGGACGCCAATCATGAGCACCGTGATGGAAATCGGCTACGACAACGTCGAATCCCAGCGCGCCGGCGACAAGAACAAT                                                                              <  SRR4302535.4632630/94‑1 (MQ=60)
                 TCGCCCGATGTACAAGTGGACGCCAATCATGAGCACCGTGATGGAAATCGGCTACGACAACGTCGAATCCCAGCGCGCCGGCGACAAGAACAAT                                                                              >  SRR4302535.2285421/1‑94 (MQ=60)
                  CGCCCGATGTACAAGTGGACGCCAATCATGAGCACCGTGATGGAAATCGGCTACGACAACGTCGACTCCCACCGCCCCGGCGACAAGAACAATC                                                                             >  SRR4302535.3773592/1‑94 (MQ=60)
                   GCCGGATGTACAAGTGGACGCCAATCATGAGCACCGTGATGGAAATCGGCTACGACAACGTCGAATCCCAGCGCGCCGGCGACAAGAACAATCA                                                                            <  SRR4302535.556851/94‑1 (MQ=60)
                    CCCGATGTACAAGTGGACGCCAATCATGAGCACCGTGATGGAAATCGTCTACGACAACGTCGAATCCCAGCGCGCCGGCGACAAGAACAATCAG                                                                           <  SRR4302535.4198389/94‑1 (MQ=60)
                    CCCGATGTACAAGTGGACGCCAATCATGAGCACCGTGATGGAAATCGGCTACGACAACGTCGAATCCCAGCGCGCCGGCGACAAGAACAATCAG                                                                           <  SRR4302535.3006308/94‑1 (MQ=60)
                    CCCGATGTACAAGTGGACGCCAATCATGAGCACCGTGATGGAAATCGGCTACGACAACGTCGAATCCCAGCGCGCCGGCGACAAGAACAATCAG                                                                           >  SRR4302535.266518/1‑94 (MQ=60)
                    CCCGATGTACAAGTGGACGCCAATCATGAGCACCGTGATGGAAATCGGCTACGACAACGTCGAATCCCAGCGCGCCGGCGACAAGAACAATCAG                                                                           <  SRR4302535.209231/94‑1 (MQ=60)
                     CCGATGTACAAGTGGACGCCAATCATGAGCACCGTGATGGAAATCGGCTACGACAACGTCGAATCCCAGCGCGCCGGCGACAAGAACAATCAGT                                                                          >  SRR4302535.3645034/1‑94 (MQ=60)
                     CCGATGTACAAGTGGACGCCAATCATGAGCACCGTGATGGAAATCGGCTACGACAACGTCGAATCCCAGCGCGCCGGCGACAAGAACAATCAGT                                                                          <  SRR4302535.1249740/94‑1 (MQ=60)
                     CCGATGTACAAGTGGACGCCAATCATGAGCACCGTGATGGAAATCGGCTACGACAACGTCGAATCCCAGCGCGCCGGCGACAAGAACAATCAGT                                                                          <  SRR4302535.413329/94‑1 (MQ=60)
                      CGATGTACAAGTGGACGCCAATCATGAGCACCGTGATGGAAATCGGCTACGACAACGTCGAATCCCAGCGCGCCGGCGACAAGAACAATCAGTA                                                                         <  SRR4302535.795985/94‑1 (MQ=60)
                      CGATGTACAAGTGGACGCCAATCATGAGCACCGTGATGGAAATCGGCTACGACAACGTCGAATCCCAGCGCGCCGGCGACAAGAACAATCAGTA                                                                         >  SRR4302535.4407034/1‑94 (MQ=60)
                      CGATGTACAAGTGGACGCCAATCATGAGCACCGTGATGGAAATCGGCTACGACAACGTCGAATCCCAGCGCGCCGGCGACAAGAACAATCAGTA                                                                         >  SRR4302535.3072221/1‑94 (MQ=60)
                       CATGTACAAGTGGACGCGAATCATGAGCACCGTGATGGAAATCGGCTACGACAACGTCGAATCCCAGCGCGCCGGCGACAAGAACAATCAGTAC                                                                        <  SRR4302535.186924/94‑1 (MQ=60)
                        ATGTACAAGTGGACGCCAATCATGAGCACCGTGATGGAAATCGGCTACGACAACGTCGAATCCCAGCGCGCCGGCGACAAGAACAATCAGTACA                                                                       >  SRR4302535.3702938/1‑94 (MQ=60)
                        ATGTACAAGTGGACGCCAATCATGAGCACCGTGATGGAAATCGGCTACGACAACGTCGAATCCCAGCGCGCCGGCGACAAGAACAATCAGTACA                                                                       >  SRR4302535.1521036/1‑94 (MQ=60)
                        ACGTACAAGTGGACGCCAATCATGAGCACCGTGATGGAAATCGGCTACGACAACGTCGAATCCCAGCGCGCCGGCGACAAGAACAATCAGTACA                                                                       <  SRR4302535.3389572/94‑1 (MQ=60)
                         TGTACAAGTGGACGCCAATCATGAGCACCGTGATGGAAATCGGCTACGACAACGTCGAATCCCAGCGCGCCGGCGACAAGAACAATCAGTACAA                                                                      >  SRR4302535.4134103/1‑94 (MQ=60)
                         TGTACAAGTGGACGCCAATCATGAGCACCGTGATGGAAATCGGCTACGACAACGTCGAATCCCAGCGCGCCGGCGACAAGAACAATCAGTACAA                                                                      <  SRR4302535.2061510/94‑1 (MQ=60)
                          GTACAAGTGGACGCCAATCATGAGCACCGTGATGGAAATCGGCTACGACAACGTCGAATCCCAGCGCGCCGGCGACAAGAACAATCAGTACAAA                                                                     <  SRR4302535.3849771/94‑1 (MQ=60)
                          GTACAAGTGGACGCCAATCATGAGCACCGTGATGGAAATCGGCTACGACAACGTCGAATCCCAGCGCGCCGGCGACAAGAACAATCAGTACAAA                                                                     >  SRR4302535.2631664/1‑94 (MQ=60)
                          GTACAAGTGGACGCCAATCATGAGCACCGTGATGGAAATCGGCTACGACAACGTCGAATCCCAGCGCGCCGGCGACAAGAAAAATCAGTACAAA                                                                     >  SRR4302535.2180922/1‑94 (MQ=60)
                             CAAGTGGACGCCAATCATGAGCACCGTGATGGAAATCGGCTACGACAACGTCGAATCCCAGCGCGCCGGCGACAAGAACAATCAGTACAAAATT                                                                  >  SRR4302535.1679584/1‑94 (MQ=60)
                             CAAGTGGACGCCAATCATGAGCACCGTGATGGAAATCGGCTACGACAACGTCGAATCCCAGCGCGCCGGCGACAAGAACAATCAGTACAAAATT                                                                  <  SRR4302535.1910607/94‑1 (MQ=60)
                              AAGTGGACGCCAATCATGAGCACCGTGATGGAAATCGGCTACGACAACGTCGAATCCCAGCGCGCCGGCGACAAGAACAATCAGTACAAAATTA                                                                 <  SRR4302535.986749/94‑1 (MQ=60)
                               AGTGGACGCCAATCATGAGCACCGTGATGGAAATCGGCTACGACAACGTCGAATCCCAGCGCGCCGGCGACAAGAACAATCAGTACAAAATTAC                                                                >  SRR4302535.1821507/1‑94 (MQ=60)
                               AGTGGACGCCAATCATGAGCACCGTGATGGAAATCGGCTACGACAACGTCGAATCCCAGCGCGCCGGCGACAAGAACAATCAGTACAAAATTAC                                                                >  SRR4302535.2623059/1‑94 (MQ=60)
                                 TGGACGCCAATCATGAGCACCGTGATGGAAATCGGCTACGACAACGTCGAATCCCAGCGCGCCGGCGACAAGAACAATCAGTACAAAATTACCC                                                              <  SRR4302535.3550194/94‑1 (MQ=60)
                                   GACGCCAATCATGAGCACCGTGATGGAAATCGGCTACGACAACGTCGAATCCCAGCGCGCCGGCGACAAGAACAATCAGTACAAAATTACCCTC                                                            <  SRR4302535.733776/94‑1 (MQ=60)
                                    ACGCCAATCATGAGCACCGTGATGGAAATCGGCTACGACAACGTCGAATCCCAGCGCGCCGGCGACAAGAACAATCAGTACAAAATTACCCTCG                                                           <  SRR4302535.3784883/94‑1 (MQ=60)
                                      GCCAATCATGAGCACCGTGATGGAAATCGGCTACGACAACGTCGAATCCCAGCGCGCCGGCGACAAGAACAATCAGTACAAAATTACCCTCGCA                                                         <  SRR4302535.2541629/94‑1 (MQ=60)
                                      GCCAATCATGAGCACCGTGATGGAAATCGGCTACGACAACGTCGAATCCCAGCGCGCCGGCGACAAGAACAATCAGTACAAAATTACCCTCGCA                                                         <  SRR4302535.198730/94‑1 (MQ=60)
                                        CAATCATGAGCACCGTGATGGAAATCGGCTACGACAACGTCGAATCCCAGCGCGCCGGCGACAAGAACAATCAGTACAAAATTACCCTCGCACA                                                       >  SRR4302535.1489356/1‑94 (MQ=60)
                                          ATCATGAGCACCGTGATGGAAATCGGCTACGACAACGTCGAATCCCAGCGCGCCGGCGACAAGAACAATCAGTACAAAATTACCCTCGCACAAA                                                     >  SRR4302535.3055690/1‑94 (MQ=60)
                                            CATGAGCACCGTGATGGAAATCGGCTACGACAACGTCGAATCCCAGCGCGCCGGCGACAAGAACAATCAGTACAAAATTACCCTCGCACAACAA                                                   >  SRR4302535.2480792/1‑94 (MQ=60)
                                             ATGAGCACCGTGATGGAAATCGGCTACGACAACGTCGAATCCCAGCGCGCCGGCGACAAGAACAATCAGTACAAAATTACCCTCGCACAACAAT                                                  <  SRR4302535.3161005/94‑1 (MQ=60)
                                             ATGAGCACCGTGATGGAAATCGGCTACGACAACGTCGAATCCCAGCGCGCCGGCGACAAGAACAATCAGTACAAAATTACCCTCGCACAACAAT                                                  >  SRR4302535.3834806/1‑94 (MQ=60)
                                             ATGAGCACCGTGATGGAAATCGGCTACGACAACGTCGAATCCCAGCGCGCCGGCGACAAGAACAATCAGTACAAAATTACCCTCGCACAACAAT                                                  >  SRR4302535.1383499/1‑94 (MQ=60)
                                             ATGAGCACCGTGATGGAAATCGGCTACGACAACGTCGAATCCCAGCGCGCCGGCGACAAGAACAATCAGTACAAAATTACCCTCGCACAACAAT                                                  >  SRR4302535.2787249/1‑94 (MQ=60)
                                              TGAGCGCCGTGATGGAAATCGGCTACGACAACGTCGAATCCCAGCGCGCCGGCGACAAGAACAATCAGTACAAAATTACCCTCGCACAACAATG                                                 <  SRR4302535.4450304/94‑1 (MQ=60)
                                              TGAGCACCGTGATGGAAATCGGCTACGACAACGTCGAATCCCAGCGCGCCGGCGACAAGAACAATCAGTACAAAATTACCCTCGCACAACAATG                                                 <  SRR4302535.1403483/94‑1 (MQ=60)
                                               GAGCACCGTGATGGAAATCGGCTACGACAACGTCGAATCCCAGCGCGCCGGCGACAAGAACAATCAGTACAAAATTACCCTCGCACAACAATGG                                                <  SRR4302535.4015137/94‑1 (MQ=60)
                                                AGCACCGTGATGGAAATCGGCTACGACAACGTCGAATCCCAGCGCGCCGGCGACAAGAACAATCAGTACAAAATTACCCTCGCACAACAATGGC                                               <  SRR4302535.1337185/94‑1 (MQ=60)
                                                AGCACCGTGATGGAAATCGGCTACGACAACGTCGAATCCCAGCGCGCCGGCGACAAGAACAATCAGTACAAAATTACCCTCGCACAACAATGGC                                               <  SRR4302535.2681922/94‑1 (MQ=60)
                                                AGCACCGTGATGGAAATCGGCTACGACAACGTCGAATCCCAGCGCGCCGGCGACAAGAACAATCAGTACAAAATTACCCTCGCACAACAATGGC                                               <  SRR4302535.471255/94‑1 (MQ=60)
                                                 GCACCGTGATGGAAATCGGCTACGACAACGTCGAATCCCAGCGCGCCGGCGACAAGAACAATCAGTACAAAATTACCCTCGCACAACAATGGCA                                              <  SRR4302535.4435141/94‑1 (MQ=60)
                                                 GCACCGTGATGGAAATCGGCTACGACAACGTCGAATCCCAGCGCGCCGGCGACAAGAACAATCAGTACAAAATTACCCTCGCACAACAATGGCA                                              <  SRR4302535.1849618/94‑1 (MQ=60)
                                                   ACCGTGATGGAAATCGGCTACGACAACGTCGAATCCCAGCGCGCCGGCGACAAGAACAATCAGTACAAAATTACCCTCGCACAACAATGGCAGG                                            <  SRR4302535.3962184/94‑1 (MQ=60)
                                                   ACCGTGATGGAAATCGGCTACGACAACGTCGAATCCCAGCGCGCCGGCGACAAGAACAATCAGTACAAAATTACCCTCGCACAACAATGGCAGG                                            <  SRR4302535.4571214/94‑1 (MQ=60)
                                                   ACCGTGATGGAAATCGGCTACGACAACGTCGAATCCCAGCGCGCCGGCGACAAGAACAATCAGTACAAAATTACCCTCGCACAACAATGGCAGG                                            <  SRR4302535.4342788/94‑1 (MQ=60)
                                                   ACCGTGATGGAAATCGGCTACGACAACGTCGAATCCCAGCGCGCCGGCGACAAGAACAATCAGTACAAAATTACCCTCGCACAACAATGGCAGG                                            <  SRR4302535.927725/94‑1 (MQ=60)
                                                   ACCGTGATGGAAATCGGCTACGACAACGTCGAATCCCAGCGCGCCGGCGACAAGAACAATCAGTACAAAATTACCCTCGCACAACAATGGCAGG                                            >  SRR4302535.2559689/1‑94 (MQ=60)
                                                   ACCGTGATGGAAATCGGCTACGACAACGTCGAATCCCAGCGCGCCGGCGACAAGAACAATCAGTACAAAATTACCCTCGCACAACAATGGCAGG                                            <  SRR4302535.475849/94‑1 (MQ=60)
                                                     CGTGATGGAAATCGGCTACGACAACGTCGAATCCCAGCGCGCCGGCGACAAGAACAATCAGTACAAAATTACCCTCGCACAACAATGGCAGGCT                                          <  SRR4302535.805960/94‑1 (MQ=60)
                                                     CGTGATGGAAATCGGCTACGACAACGTCGAATCCCAGCGCGCCGGCGACAAGAACAATCAGTACAAAATTACCCTCGCACAACAATGGCAGGCT                                          >  SRR4302535.3566249/1‑94 (MQ=60)
                                                       TGATGGAAATCGGCTACGACAACGTCGAATCCCAGCGCGCCGGCGACAAGAACAATCAGTACAAAATTACCCTCGCACAACAATGGCAGGCTGG                                        >  SRR4302535.4118271/1‑94 (MQ=60)
                                                        GATGGAAATCGGCTACGACAACGTCGAATCCCAGCGCGCCGGCGACAAGAACAATCAGTACAAAATTACCCTCGCACAACAATGGCAGGCTGGC                                       >  SRR4302535.2602957/1‑94 (MQ=60)
                                                         ATGGAAATCGGCTACGACAACGTCGAATCCCAGCGCGCCGGCGACAAGAACAATCAGTACAAAATTACCCTCGCACAACAATGGCAGGCTGGCG                                      >  SRR4302535.2529134/1‑94 (MQ=60)
                                                           GGAAATCGGCTACGACAACGTCGAATCCCAGCGCGCCGGCGACAAGAACAATCAGTACAAAATTACCCTCGCACAACAATGGCAGGCTGGCGAC                                    <  SRR4302535.4160201/94‑1 (MQ=60)
                                                           GGAAATCGGCTACGACAACGTCGAATCCCAGCGCGCCGGCGACAAGAACAATCAGTACAAAATTACCCTCGCACAACAATGGCAGGCTGGCGAC                                    <  SRR4302535.2332633/94‑1 (MQ=60)
                                                              AATCGGCTACGACAACGTCGAATCCCAGCGCGCCGGCGACAAGAACAATCAGTACAAAATTACCCTCGCACAACAATGGCAGGCTGGCGACAGC                                 <  SRR4302535.2801932/94‑1 (MQ=60)
                                                               ATCGTCTACGACAACGTCGAATCCCAGCGCGCCGGCGACAAGAACAATCAGTACAAAATTACCCTCGCACAACAATGGCAGGCTGGCGACAGCA                                <  SRR4302535.4501625/94‑1 (MQ=60)
                                                                TCGGCTACGACAACGTCGAATCCCAGCGCGCCGGCGACAAGAACAATCAGTACAAAATTACCCTCGCACAACAATTGCAGGCTGGCGACAGCAT                               <  SRR4302535.2208970/94‑1 (MQ=60)
                                                                TCGGCTACGACAACGTCGAATCCCAGCGCGCCGGCGACAAGAACAATCAGTACAAAATTACCCTCGCACAACAATGGCAGGCTGGCGACAGCAT                               <  SRR4302535.3152944/94‑1 (MQ=60)
                                                                 CGACTACGACAACGTCGAATCCCAGCGCGCCGGCGACAAGAACAATCAGTACAAAATTACCCTCGCAAAACAATGGCAGGCTGGCGACAGCATC                              <  SRR4302535.2326586/94‑1 (MQ=60)
                                                                  GGCTACGACAACGTCGAATCCCAGCGCGCCGGCGACAAGAACAATCAGTACAAAATTACCCTCGCACAACAATGGCAGGCTGGCGACAGCATCT                             <  SRR4302535.340098/94‑1 (MQ=60)
                                                                   GCTACGACAACGTCGAATCCCAGCGCGCCGGCGACAAGAACAATCAGTACAAAATTACCCTCGCACAACAATGGCAGGCTGGCGACAGCATCTG                            <  SRR4302535.4253483/94‑1 (MQ=60)
                                                                   GCTACGACAACGTCGAATCCCAGCGCGCCGGCGACAAGAACAATCAGTACAAAATTACCCTCGCACAACAATGGCAGGCTGGCGACAGCATCTG                            <  SRR4302535.166741/94‑1 (MQ=60)
                                                                    CTACGACAACGTCGAATCCCAGCGCGCCGGCGACAAGAACAATCAGTACAAAATTACCCTCGCACAACAATGGCAGGCTGGCGACAGCATCTGG                           <  SRR4302535.2863014/94‑1 (MQ=60)
                                                                     TACGACAACGTCGAATCCCAGCGCGCCGGCGACAAGAACAATCAGTACAAAATTCCCCTCGCACAACAATGGCAGGCTGGCGACAGCATCTGGT                          <  SRR4302535.2610914/94‑1 (MQ=60)
                                                                     TACGACAACGTCGAATCCCAGCGCGCCGGCGACAAGAACAATCAGTACAAAATTACCCTCGCACAACAATGGCAGGCTGGCGACAGCATCTGGT                          >  SRR4302535.2870086/1‑94 (MQ=60)
                                                                     TACGACAACGTCGAATCCCAGCGCGCCGGCGACAAGAACAATCAGTACAAAATTACCCTCGCACAACAATGGCAGGCTGGCGACAGCATCTGGT                          <  SRR4302535.3852795/94‑1 (MQ=60)
                                                                        GACAACGTCGAATCCCAGCGCGCCGGCGACAAGAACAATCAGTACAAAATTACCCTCGCACAACAATGGCAGGCTGGCGACAGCATCTGGTCAC                       >  SRR4302535.4146567/1‑94 (MQ=60)
                                                                        GACAACGTCGAATCCCAGCGCGCCGGCGACAAGAACAATCAGTACAAAATTACCCTCGCACAACAATGGCAGGCTGGCGACAGCATCTGGTCAC                       >  SRR4302535.2629948/1‑94 (MQ=60)
                                                                         ACAACGTCGAATCCCAGCGCGCCGGCGACAAGAACAATCAGTACAAAATTACCCTCGCACAACAATGGCAGGCTGGCGACAGCATCTGGTCACG                      >  SRR4302535.2878240/1‑94 (MQ=60)
                                                                             CGTCGAATCCCAGCGCGCCGGCGACAAGAACAATCAGTACAAAATTACCCTCGCACAACAATGACAGGCTGGCGACAGCATCTGGTCACGCCCG                  <  SRR4302535.1789265/94‑1 (MQ=60)
                                                                              GTCGAATCCCAGCGCGCCGGCGACAAGAACAATCAGTACAAAATTCCCCTCGCACAACAATGGCAGGCTGGCGACAGCATCTGGTCACGCCCGG                 <  SRR4302535.640162/94‑1 (MQ=60)
                                                                              GTCGAATCCCAGCGCGCCGGCGACAAGAACAATCAGTACAAAATTACCCTCGCACAACAATGGCAGGCTGGCGACAGCATCTGGTCACGCCCGG                 >  SRR4302535.746496/1‑94 (MQ=60)
                                                                              GTCGAATCCCAGCGCGCCGGCGACAAGAACAATCAGTACAAAATTACCCTCGCACAACAATGGCAGGCTGGCGACAGCATCTGGTCACGCCCGG                 <  SRR4302535.76469/94‑1 (MQ=60)
                                                                                CGAATCCCAGCGCGCCGGCGACAAGAACAATCAGTACAAAATTACCCTCGCACAACAATGGCAGGCTGGCGACCGCATCTGGTCACGCCCGGCT               >  SRR4302535.1432644/1‑94 (MQ=60)
                                                                                CGAATCCCAGCGCGCCGGCGACAAGAACAATCAGTACAAAATTACCCTCGCACAACAATGGCAGGCTGGCGACAGCATCTGGTCACGCCCGGCT               <  SRR4302535.2433718/94‑1 (MQ=60)
                                                                                  AATCCCAGCGCGCCGGCGACAAGAACAATCAGTACAAAATTACCCTCGCACAACAATGGCAGGCTGGCGACAGCATCTGGTCACGCCCGGCTAT             <  SRR4302535.236509/94‑1 (MQ=60)
                                                                                  AATCCCAGCGCGCCGGCGACAAGAACAATCAGTACAAAATTACCCTCGCACAACAATGGCAGGCTGGCGACAGCATCTGGTCACGCCCGGCTAT             >  SRR4302535.2077616/1‑94 (MQ=60)
                                                                                    TCCCAGCGCGCCGGCGACAAGAACAATCAGTACAAAATTACCCTCGCACAACAATGGCAGGCTGGCGACAGCATCTGGTCACGCCCGGCTATTC           <  SRR4302535.907602/94‑1 (MQ=60)
                                                                                     CCCAGCGCGCCGGCGACAAGAACAATCAGTACAAAATTACCCTCGCACAACAATGGCAGGCTGGCGACAGCATCTGGTCACGCCCGGCTATTCG          <  SRR4302535.997093/94‑1 (MQ=60)
                                                                                     CCCAGCGCGCCGGCGACAAGAACAATCAGTACAAAATTACCCTCGCACAACAATGGCAGGCTGGCGACAGCATCTGGTCACGCCCGGCTATTCG          <  SRR4302535.1299775/94‑1 (MQ=60)
                                                                                     CCCAGCGCGCCGGCGACAAGAACAATCAGTACAAAATTACCCTCGCACAACAATGGCAGGCTGGCGACAGCATCTGGTCACGCCCGGCTATTCG          <  SRR4302535.372517/94‑1 (MQ=60)
                                                                                      CCAGCGCGCCGGCGACAAGAACAATCAGTACAAAATTACCCTCGCACAACAATGGCAGGCTGGCGACAGCATCTGGTCACGCCCGGCTATTCGT         >  SRR4302535.1145066/1‑94 (MQ=60)
                                                                                         GCGCGCCGGCGACAAGAACAATCAGTACAAAATTACCCTCGCACAACAATGGCAGGCTGGCGACAGCATCTGGTCACGCCCGGCTATTCGTGTC      <  SRR4302535.1004845/94‑1 (MQ=60)
                                                                                          CGCGCCGGCGACAAGAACAATCAGTACAAAATTACCCTCGCACAACAATGGCAGGCTGGCGACAGCATCTGGTCACGCCCGGCTATTCGTGTCT     <  SRR4302535.3996282/94‑1 (MQ=60)
                                                                                            CGCCGGCGACAAGAACAATCAGTACAAAATTACCCTCGCACAACAATGGCAGGCTGGCGACAGCATCTGGTCACGCCCGGCTATTCGCGTCTTC   >  SRR4302535.1998901/1‑94 (MQ=60)
                                                                                             GCCGGCGACAAGAACAATCAGTACAAAATTACCCTCGCACAACAATGGCAGGCTGGCGACAGCATCTGGTCACGCCCGGCTATTCGTGTCTTCG  <  SRR4302535.2521920/94‑1 (MQ=60)
                                                                                             |                                                                                             
TGGTGGACCGTCGGTATTCGCCCGATGTACAAGTGGACGCCAATCATGAGCACCGTGATGGAAATCGGCTACGACAACGTCGAATCCCAGCGCACCGGCGACAAGAACAATCAGTACAAAATTACCCTCGCACAACAATGGCAGGCTGGCGACAGCATCTGGTCACGCCCGGCTATTCGTGTCTTCG  >  NC_000913/4246537‑4246723

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑