Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1873 183.1 9376148 98.2% 9207377 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,250,949 A→G I119V (ATT→GTT)  ubiA → 4‑hydroxybenzoate‑octaprenyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,250,9490AG100.0% 738.6 / NA 230I119V (ATT→GTT) ubiA4‑hydroxybenzoate‑octaprenyltransferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (125/105);  total (125/105)

Only 100 of 234 total aligned reads displayed.

GGCGCGGTAACAGAGAAAGAGGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCATTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTACCGCAAGTGGTGCTGGGCGC  >  NC_000913/4250859‑4251037
                                                                                          |                                                                                        
ggcgCGGTAACAGAGAAAGAGGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGtt                                                                                        >  1:350358/1‑93 (MQ=255)
 gcgcGGTAACAGAGAAAGAGGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTc                                                                                       <  2:2367260/93‑1 (MQ=255)
      gTAACAGAGAAAGAGGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCtgttg                                                                                  >  2:1067605/1‑93 (MQ=255)
       tAACAGAGAAAGAGGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCtgttgt                                                                                 <  2:2054792/93‑1 (MQ=255)
        aaCAGAGAAAGAGGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTc                                                                                >  2:1230690/1‑93 (MQ=255)
         aCAGAGAAAGAGGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCg                                                                               >  1:3580078/1‑93 (MQ=255)
          cAGAGAAAGA‑GCGCGGGGCGTGTTTGTTGTGCTGGTTCTGATTTCGTTTTTACTGGGTCTTACGCTGAAAACGATGACCGTTCTGTTGTCGAt                                                                             <  2:1288373/93‑1 (MQ=255)
           agagAAAGAGGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGAt                                                                             <  2:575942/93‑1 (MQ=255)
           agagAAAGAGGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGAt                                                                             <  2:4571370/93‑1 (MQ=255)
           agagAAAGAGGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGAt                                                                             >  1:54412/1‑93 (MQ=255)
            gagaAAGAGGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGAtt                                                                            >  1:4382042/1‑93 (MQ=255)
            gagaAAGAGGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGAtt                                                                            <  2:3643265/93‑1 (MQ=255)
            gagaAAGAGGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGAtt                                                                            >  2:2925898/1‑93 (MQ=255)
            gagaAAGAGGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGAtt                                                                            >  2:1496088/1‑93 (MQ=255)
              gaAAGAGGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGc                                                                          >  2:2696197/1‑93 (MQ=255)
               aaaGAGGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGcc                                                                         >  1:4121815/1‑93 (MQ=255)
               aaaGAGGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGcc                                                                         >  1:2098537/1‑93 (MQ=255)
                aaGAGGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGGTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCcg                                                                        >  2:3993148/1‑93 (MQ=255)
                 agagGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCcgc                                                                       <  1:2050557/93‑1 (MQ=255)
                 agagGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCcgc                                                                       >  2:2855858/1‑93 (MQ=255)
                 agagGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCcgc                                                                       <  2:1022191/93‑1 (MQ=255)
                 agagGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCcgc                                                                       >  2:50861/1‑93 (MQ=255)
                  gagGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCcgcg                                                                      >  2:1114098/1‑93 (MQ=255)
                   agGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCgcgc                                                                     >  1:1713584/1‑93 (MQ=255)
                    ggCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCt                                                                    >  1:587775/1‑93 (MQ=255)
                     gcgcgcgcgcTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTa                                                                   <  2:228311/93‑1 (MQ=255)
                      cgcgcgcgcTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAg                                                                  <  1:4264682/93‑1 (MQ=255)
                       gcgcgcgcTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAgg                                                                 >  1:118754/1‑92 (MQ=255)
                         gcgcgcTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAgcgc                                                               <  1:1100518/93‑1 (MQ=255)
                          cgcgcTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCt                                                              <  1:264945/93‑1 (MQ=255)
                            cgcTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTgg                                                            <  2:2907675/93‑1 (MQ=255)
                            cgcTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTgg                                                            >  1:1667884/1‑93 (MQ=255)
                             gcTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGc                                                           >  2:4316211/1‑93 (MQ=255)
                               tGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGt                                                         <  1:329765/93‑1 (MQ=255)
                                gTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTg                                                        <  1:2733120/93‑1 (MQ=255)
                                gTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTg                                                        >  2:4488720/1‑93 (MQ=255)
                                 tttGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTgg                                                       >  1:3978222/1‑93 (MQ=255)
                                 tttGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTgg                                                       >  2:3300850/1‑93 (MQ=255)
                                   tGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGgt                                                     <  2:339158/93‑1 (MQ=255)
                                    gTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGCCCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGgtg                                                    <  1:4335364/93‑1 (MQ=255)
                                    gTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGgtg                                                    <  1:763031/93‑1 (MQ=255)
                                     tCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGgtgt                                                   >  1:4575581/1‑93 (MQ=255)
                                       gTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTAc                                                 <  1:3144761/93‑1 (MQ=255)
                                       gTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTAc                                                 <  1:1528374/93‑1 (MQ=255)
                                        tGCTCGTACTGATTGCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTAcc                                                <  1:1285389/93‑1 (MQ=255)
                                          cTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGAGTGTACCCg                                              >  2:3758654/1‑93 (MQ=255)
                                           tGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGt                                             >  2:4163439/1‑93 (MQ=255)
                                           tGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGt                                             >  1:278760/1‑93 (MQ=255)
                                            ggTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGtt                                            >  1:3204387/1‑93 (MQ=255)
                                             gTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGttt                                           <  1:4618678/93‑1 (MQ=255)
                                              tACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTa                                          >  2:315869/1‑93 (MQ=255)
                                              tACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTa                                          <  2:2974663/93‑1 (MQ=255)
                                              tACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTa                                          <  2:8624/93‑1 (MQ=255)
                                               aCTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTAt                                         >  2:1990605/1‑93 (MQ=255)
                                               aCTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTAt                                         >  2:379331/1‑93 (MQ=255)
                                               aCTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTAt                                         >  1:1016699/1‑93 (MQ=255)
                                                cTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATg                                        >  2:3118718/1‑93 (MQ=255)
                                                cTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATg                                        <  1:4440650/93‑1 (MQ=255)
                                                 tGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGa                                       >  1:3740245/1‑93 (MQ=255)
                                                  gATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGaa                                      >  1:2861231/1‑93 (MQ=255)
                                                  gATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGaa                                      <  2:1782542/93‑1 (MQ=255)
                                                  gATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGaa                                      <  2:1846982/93‑1 (MQ=255)
                                                   aTTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAg                                     >  2:79686/1‑93 (MQ=255)
                                                   aTTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAg                                     >  1:3817443/1‑93 (MQ=255)
                                                    tttCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAgg                                    >  1:2317074/1‑92 (MQ=255)
                                                    tttCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGc                                    >  1:2549043/1‑93 (MQ=255)
                                                    tttCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGc                                    >  2:1940873/1‑93 (MQ=255)
                                                    tttCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGc                                    >  1:661934/1‑93 (MQ=255)
                                                     ttCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCg                                   >  1:2590252/1‑93 (MQ=255)
                                                      tCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCgg                                  >  2:1040881/1‑93 (MQ=255)
                                                       cGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGt                                 <  1:797433/93‑1 (MQ=255)
                                                        gTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGta                                >  2:3741208/1‑93 (MQ=255)
                                                        gTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGta                                <  2:1136131/93‑1 (MQ=255)
                                                          ttttACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGtata                              >  1:411209/1‑93 (MQ=255)
                                                           tttACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATAc                             <  2:3906286/93‑1 (MQ=255)
                                                             tACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATAccc                           >  2:2437783/1‑93 (MQ=255)
                                                             tACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATAccc                           <  1:4048209/93‑1 (MQ=255)
                                                              aCTGGTGCTGACTCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCa                          <  1:2032726/93‑1 (MQ=255)
                                                               cTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCAt                         <  1:897186/93‑1 (MQ=255)
                                                                tGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCCTc                        >  2:2621544/1‑93 (MQ=255)
                                                                  gTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTa                      <  1:2158751/93‑1 (MQ=255)
                                                                   tGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTCCCCGTTTATGAAGCGGTATACCCATCTAc                     <  2:408447/93‑1 (MQ=255)
                                                                       gACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTACCGCa                 >  2:3083688/1‑93 (MQ=255)
                                                                        aCGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTACCGCaa                <  2:1758210/93‑1 (MQ=255)
                                                                        aCGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTACCGCaa                >  2:164249/1‑93 (MQ=255)
                                                                        aCGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGAGTAACACTCTACCGctg                >  1:138779/1‑91 (MQ=255)
                                                                         cGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTACCGCAAg               >  2:1456560/1‑93 (MQ=255)
                                                                          gCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTACCGCAAgt              >  2:1101787/1‑93 (MQ=255)
                                                                          gCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTACCGCAAgt              >  1:3357313/1‑93 (MQ=255)
                                                                          gCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTACCGCAAgt              >  1:3086341/1‑93 (MQ=255)
                                                                              aaTACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTACCGCAAgtggtg          >  1:1322863/1‑93 (MQ=255)
                                                                               aTACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTACCGCAAGTGGTGc         <  2:4321557/93‑1 (MQ=255)
                                                                                tACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTACCGCAAGTGGTGCt        <  1:440003/93‑1 (MQ=255)
                                                                                tACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTACCGCAAGTGGTGCt        >  2:682432/1‑93 (MQ=255)
                                                                                 aCGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTACCGCAAGTGGTGCTg       <  1:1926234/93‑1 (MQ=255)
                                                                                   gATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTACCGCAAGTGGTGCTggg     >  2:3820369/1‑93 (MQ=255)
                                                                                   gATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAACGGTATACCCCTCTAACGCAAGAGGTGCTggg     >  2:928106/1‑93 (MQ=255)
                                                                                    aTGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTACCGCAAGTGGTGCTGGgc    <  2:3395394/93‑1 (MQ=255)
                                                                                      gACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTACCGCAAGTGGTGCTGGgcgc  >  1:609712/1‑93 (MQ=255)
                                                                                      gACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTACCGCAAGTGGTGCTGGgcgc  <  2:429053/93‑1 (MQ=255)
                                                                                          |                                                                                        
GGCGCGGTAACAGAGAAAGAGGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCATTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTACCGCAAGTGGTGCTGGGCGC  >  NC_000913/4250859‑4251037

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AGCGGCGCGGTAACAGAGAAAGAGGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCATTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTACCGCAAGTGGTGCTGGGCGCGGCGT  >  NC_000913/4250856‑4251042
                                                                                             |                                                                                             
AGCGGCGCGGTAACAGAGAAAGAGGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCG                                                                                               >  SRR4302535.250560/1‑94 (MQ=60)
AGCGGCGCGGTAACAGAGAAAGAGGCGCGCGCGCTGGTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCG                                                                                               >  SRR4302535.682676/1‑94 (MQ=60)
 GCGGCGCGGTAACAGAGAAAGAGGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGT                                                                                              <  SRR4302535.4310269/94‑1 (MQ=60)
  CGGCGCGGTAACAGAGAAAGAGGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTT                                                                                             >  SRR4302535.352773/1‑94 (MQ=60)
    GCGCGGTAACAGAGAAAGAGGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGTCCGTTCT                                                                                           <  SRR4302535.1570864/94‑1 (MQ=60)
    GCGCGGTAACAGAGAAAGAGGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCT                                                                                           <  SRR4302535.4493411/94‑1 (MQ=60)
     CGCGGTAACAGAGAAAGAGGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTG                                                                                          <  SRR4302535.2401327/94‑1 (MQ=60)
      GCGGTAACAGAGAAAGAGGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGT                                                                                         >  SRR4302535.2713290/1‑94 (MQ=60)
       CGGTAACAGAGAAAGAGGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTT                                                                                        <  SRR4302535.170292/94‑1 (MQ=60)
         GTAACAGAGAAAGAGGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGT                                                                                      >  SRR4302535.3139355/1‑94 (MQ=60)
          TAACAGAGAAAGAGGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTC                                                                                     <  SRR4302535.4350536/94‑1 (MQ=60)
           AACAGAGAAAGAGGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCG                                                                                    <  SRR4302535.3568391/94‑1 (MQ=60)
           AACAGAGAAAGAGGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCG                                                                                    >  SRR4302535.3604891/1‑94 (MQ=60)
             CAGAGAAAGAGGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGAT                                                                                  >  SRR4302535.54811/1‑94 (MQ=60)
             CAGAGAAAGAGGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGAT                                                                                  >  SRR4302535.4395394/1‑94 (MQ=60)
              AGAGAAAGAGGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATT                                                                                 <  SRR4302535.601073/94‑1 (MQ=60)
              AGAGAAAGAGGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATT                                                                                 >  SRR4302535.4412020/1‑94 (MQ=60)
              AGAGAAAGAGGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATT                                                                                 >  SRR4302535.2678751/1‑94 (MQ=60)
               GAGAAAGAGGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTC                                                                                >  SRR4302535.721562/1‑94 (MQ=60)
                AGAAAGAGGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGC                                                                               <  SRR4302535.4384704/94‑1 (MQ=60)
                 GAAAGAGGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCC                                                                              >  SRR4302535.4150020/1‑94 (MQ=60)
                 GAAAGAGGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCC                                                                              >  SRR4302535.2113672/1‑94 (MQ=60)
                    AGAGGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCG                                                                           <  SRR4302535.2065323/94‑1 (MQ=60)
                     GAGGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGC                                                                          >  SRR4302535.1725974/1‑94 (MQ=60)
                      AGGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCT                                                                         >  SRR4302535.591895/1‑94 (MQ=60)
                       GGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTA                                                                        <  SRR4302535.3789690/94‑1 (MQ=60)
                         CGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGG                                                                      >  SRR4302535.119586/1‑94 (MQ=60)
                         CGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGC                                                                      <  SRR4302535.4293888/94‑1 (MQ=60)
                          GCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCG                                                                     >  SRR4302535.3563775/1‑94 (MQ=60)
                            GCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCT                                                                   <  SRR4302535.1351252/94‑1 (MQ=60)
                            GCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCT                                                                   <  SRR4302535.4018560/94‑1 (MQ=60)
                            GCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCT                                                                   <  SRR4302535.1108378/94‑1 (MQ=60)
                             CGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTG                                                                  <  SRR4302535.266785/94‑1 (MQ=60)
                             CGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTG                                                                  >  SRR4302535.2586718/1‑94 (MQ=60)
                              GCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGG                                                                 >  SRR4302535.1471810/1‑94 (MQ=60)
                              GCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGG                                                                 >  SRR4302535.1679954/1‑94 (MQ=60)
                               CGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGC                                                                <  SRR4302535.798782/94‑1 (MQ=60)
                               CGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGC                                                                <  SRR4302535.1839254/94‑1 (MQ=60)
                               CGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGC                                                                >  SRR4302535.4427405/1‑94 (MQ=60)
                                GCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCG                                                               >  SRR4302535.4148511/1‑94 (MQ=60)
                                 CTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGT                                                              <  SRR4302535.4147547/94‑1 (MQ=60)
                                  TGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTG                                                             <  SRR4302535.332052/94‑1 (MQ=60)
                                  TGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTG                                                             >  SRR4302535.1871337/1‑94 (MQ=60)
                                   GTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGG                                                            >  SRR4302535.4005405/1‑94 (MQ=60)
                                   GTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGG                                                            <  SRR4302535.2752627/94‑1 (MQ=60)
                                    TTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGG                                                           <  SRR4302535.3402660/94‑1 (MQ=60)
                                      TGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTG                                                         >  SRR4302535.280368/1‑94 (MQ=60)
                                      TGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTG                                                         >  SRR4302535.2097327/1‑94 (MQ=60)
                                       GTCGTGCTGGTACTGATTTCGTTTTTCCTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGT                                                        <  SRR4302535.3973149/94‑1 (MQ=60)
                                       GTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGCCCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGT                                                        <  SRR4302535.4365016/94‑1 (MQ=60)
                                       GTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGT                                                        >  SRR4302535.4606762/1‑94 (MQ=60)
                                       GTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGT                                                        <  SRR4302535.768407/94‑1 (MQ=60)
                                         CGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTAC                                                      <  SRR4302535.1300669/94‑1 (MQ=60)
                                         CGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTAC                                                      <  SRR4302535.2397941/94‑1 (MQ=60)
                                          GTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACC                                                     <  SRR4302535.1539385/94‑1 (MQ=60)
                                          GTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACC                                                     <  SRR4302535.3166632/94‑1 (MQ=60)
                                           TGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCC                                                    >  SRR4302535.1037631/1‑94 (MQ=60)
                                           TGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCC                                                    <  SRR4302535.1231967/94‑1 (MQ=60)
                                           TGCTCGTACTGATTGCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCC                                                    <  SRR4302535.1294550/94‑1 (MQ=60)
                                             CTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGT                                                  >  SRR4302535.280703/1‑94 (MQ=60)
                                              TGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTT                                                 >  SRR4302535.3226578/1‑94 (MQ=60)
                                              TGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTT                                                 >  SRR4302535.1117579/1‑94 (MQ=60)
                                              TGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTT                                                 >  SRR4302535.2830262/1‑94 (MQ=60)
                                                GTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTA                                               <  SRR4302535.4650062/94‑1 (MQ=60)
                                                GTACTGATTTCGTTTTTACTGGAGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTA                                               <  SRR4302535.4575900/94‑1 (MQ=60)
                                                 TACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTAT                                              <  SRR4302535.2310492/94‑1 (MQ=60)
                                                 TACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTAT                                              >  SRR4302535.2022642/1‑94 (MQ=60)
                                                 TACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTAT                                              >  SRR4302535.1023924/1‑94 (MQ=60)
                                                  ACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATG                                             <  SRR4302535.3221137/94‑1 (MQ=60)
                                                  ACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATG                                             <  SRR4302535.2081417/94‑1 (MQ=60)
                                                   CTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGA                                            <  SRR4302535.4471089/94‑1 (MQ=60)
                                                   CTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGA                                            >  SRR4302535.583632/1‑94 (MQ=60)
                                                   CTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGA                                            <  SRR4302535.2643126/94‑1 (MQ=60)
                                                   CTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGA                                            >  SRR4302535.3766131/1‑94 (MQ=60)
                                                   CTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGA                                            >  SRR4302535.2969201/1‑94 (MQ=60)
                                                    TGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAA                                           >  SRR4302535.2881499/1‑94 (MQ=60)
                                                    TGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAA                                           >  SRR4302535.3289784/1‑94 (MQ=60)
                                                     GATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAG                                          >  SRR4302535.3843789/1‑94 (MQ=60)
                                                      ATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGG                                         >  SRR4302535.2333656/1‑94 (MQ=60)
                                                      ATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGC                                         >  SRR4302535.2567385/1‑94 (MQ=60)
                                                      ATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGC                                         >  SRR4302535.666554/1‑94 (MQ=60)
                                                       TTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCG                                        >  SRR4302535.2608852/1‑94 (MQ=60)
                                                       TTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCG                                        >  SRR4302535.811553/1‑94 (MQ=60)
                                                       TTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCG                                        <  SRR4302535.3339242/94‑1 (MQ=60)
                                                         TCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGT                                      <  SRR4302535.3313791/94‑1 (MQ=60)
                                                          CGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTA                                     <  SRR4302535.803032/94‑1 (MQ=60)
                                                           GTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTAT                                    <  SRR4302535.2921057/94‑1 (MQ=60)
                                                            TTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATA                                   >  SRR4302535.414097/1‑94 (MQ=60)
                                                               TTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCC                                <  SRR4302535.588519/94‑1 (MQ=60)
                                                                TACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCA                               <  SRR4302535.4075919/94‑1 (MQ=60)
                                                                TACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCA                               >  SRR4302535.4433044/1‑94 (MQ=60)
                                                                 ACTGGTGCTGACTCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCAT                              <  SRR4302535.2047375/94‑1 (MQ=60)
                                                                  CTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATC                             >  SRR4302535.2118458/1‑94 (MQ=60)
                                                                  CTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATC                             <  SRR4302535.903545/94‑1 (MQ=60)
                                                                   TGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCT                            <  SRR4302535.981713/94‑1 (MQ=60)
                                                                     GTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTAC                          >  SRR4302535.3958267/1‑94 (MQ=60)
                                                                     GTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTAC                          <  SRR4302535.2174261/94‑1 (MQ=60)
                                                                       GCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTACCG                        >  SRR4302535.362894/1‑94 (MQ=60)
                                                                          GACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGagtaacactctaccgctg                     >  SRR4302535.139751/1‑76 (MQ=60)
                                                                            CGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTACCGCAAGT                   <  SRR4302535.4228400/94‑1 (MQ=60)
                                                                            CGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTACCGCAAGT                   >  SRR4302535.3380687/1‑94 (MQ=60)
                                                                            CGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTACCGCAAGT                   >  SRR4302535.3107835/1‑94 (MQ=60)
                                                                             GCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTACCGCAAGTG                  >  SRR4302535.4092427/1‑94 (MQ=60)
                                                                              CTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTACCGCAAGTGG                 >  SRR4302535.4710554/1‑94 (MQ=60)
                                                                              CTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTACCGCAAGTGG                 >  SRR4302535.4607649/1‑94 (MQ=60)
                                                                               TGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTACCGCAAGTGGT                >  SRR4302535.2058669/1‑94 (MQ=60)
                                                                               TGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTACCGCAAGTGGT                <  SRR4302535.644041/94‑1 (MQ=60)
                                                                                GAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTACCGCAAGTGGTG               >  SRR4302535.1332305/1‑94 (MQ=60)
                                                                                 AATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTACCGCAAGTGGTGC              <  SRR4302535.120971/94‑1 (MQ=60)
                                                                                  ATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTACCGCAAGTGGTGCT             >  SRR4302535.1533222/1‑94 (MQ=60)
                                                                                  ATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTACCGCAAGTGGTGCT             <  SRR4302535.69682/94‑1 (MQ=60)
                                                                                   TACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTACCGCAAGTGGTGCTG            <  SRR4302535.443085/94‑1 (MQ=60)
                                                                                    ACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTACCGCAAGTGGTGCTGG           <  SRR4302535.1940117/94‑1 (MQ=60)
                                                                                        TGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTACCGCAAGTGGTGCTGGGCGC       >  SRR4302535.613963/1‑94 (MQ=60)
                                                                                          ACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTACCGCAAGTGGTGCTGGGCGCGG     >  SRR4302535.3387776/1‑94 (MQ=60)
                                                                                            CGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTACCGCAAGTGGTGCTGGGCGCGGCG   >  SRR4302535.1072073/1‑94 (MQ=60)
                                                                                             GTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTACCGCAAGTGGTGCTGGGCGCGGCGT  <  SRR4302535.2083335/94‑1 (MQ=60)
                                                                                             GTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTACCGCAAGTGGTGCTGGGCGCGGCGT  >  SRR4302535.4375802/1‑94 (MQ=60)
                                                                                             |                                                                                             
AGCGGCGCGGTAACAGAGAAAGAGGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCATTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTACCGCAAGTGGTGCTGGGCGCGGCGT  >  NC_000913/4250856‑4251042

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑