Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1873 183.1 9376148 98.2% 9207377 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,388,932 A→G A52A (GCT→GCC)  b4161 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,388,9320AG99.5% 637.1 / NA 207A52A (GCT→GCC) b4161hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base G (82/123);  total (84/123)
Fisher's exact test for biased strand distribution p-value = 4.03e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 218 total aligned reads displayed.

ACGCCAGACTACGCGGTCGCCGGTTACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCAGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCGGGCTTCTCCTTAGACGTTTT  >  NC_000913/4388842‑4389021
                                                                                          |                                                                                         
aCGCCAGACTACGCGGTCGCCGGTTACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGc                                                                                         <  2:1159725/93‑1 (MQ=255)
 cGCCAGACTACGCGGTCGCCGGTTACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCg                                                                                        >  1:4157472/1‑93 (MQ=255)
 cGCCAGACTACGCGGTCGCCGGTTACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCg                                                                                        <  2:2861375/93‑1 (MQ=255)
  gCCAGACTACGCGGTCGCCGGTTACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGt                                                                                       <  2:238381/93‑1 (MQ=255)
  gCCAGACTACGCGGTCGCCGGTTACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGt                                                                                       <  1:255125/93‑1 (MQ=255)
  gCCAGACTACGCGGTCGCCGGTTACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGt                                                                                       >  2:776829/1‑93 (MQ=255)
  gCCAGACTACGCGGTCGCCGGTTACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGt                                                                                       <  1:2023878/93‑1 (MQ=255)
   ccAGACTACGCGGTCGCCGGTTACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTg                                                                                      <  2:4269833/93‑1 (MQ=255)
    cAGACTACGCGGTCGCCGGTTACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGc                                                                                     >  2:1880841/1‑93 (MQ=255)
    cAGACTACGCGGTCGCCGGTTACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGc                                                                                     >  2:3303026/1‑93 (MQ=255)
     aGACTACGCGGTCGCCGGTTACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCa                                                                                    <  2:255936/93‑1 (MQ=255)
      gACTACGCGGTCGCCGGTTACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCAt                                                                                   <  1:513875/93‑1 (MQ=255)
      gACTACGCGGTCGCCGGTTACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCAt                                                                                   >  2:3158482/1‑93 (MQ=255)
      gACTACGCGGTCGCCGGTTACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACACCGGCGGGCaa                                                                                   >  1:4196494/1‑92 (MQ=255)
       aCTACGCGGTCGCCGGTTACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCAtc                                                                                  >  1:2801510/1‑92 (MQ=255)
       aCTACGCGGTCGCCGGTTACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATa                                                                                  <  2:4243840/93‑1 (MQ=255)
        cTACGCGGTCGCCGGTTACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATAc                                                                                 <  1:3095279/93‑1 (MQ=255)
        cTACGCGGTCGCCGGTTACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGGGCATAc                                                                                 >  2:2211229/1‑93 (MQ=255)
         tACGCGGTCGCCGGTTACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGTTTCCACATCCGAGTGCATAcc                                                                                >  1:4361338/1‑93 (MQ=255)
         tACGCGGTCGCCGGTTACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATAcc                                                                                <  2:4116099/93‑1 (MQ=255)
         tACGCGGTCGCCGGTTACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATAcc                                                                                >  1:4277845/1‑93 (MQ=255)
         tACGCGGTCGCCGGTTACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATAcc                                                                                <  1:3780552/93‑1 (MQ=255)
           cgcgGTCGCCGGTTACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCaa                                                                              <  1:3021744/93‑1 (MQ=255)
           cgcgGTCGCCGGTTACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCaa                                                                              <  2:2731237/93‑1 (MQ=255)
           cgcgGTCGCCGGTTACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCaa                                                                              <  1:1298863/93‑1 (MQ=255)
            gcgGTCGCCGGTTACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCaaa                                                                             <  1:2004383/93‑1 (MQ=255)
             cgGTCGCCGGTTACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAg                                                                            >  2:812420/1‑93 (MQ=255)
             cgGTCGCCGGTTACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAg                                                                            >  1:1623947/1‑93 (MQ=255)
              ggTCGCCGGTTACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGc                                                                           <  2:2645090/93‑1 (MQ=255)
                  gCCGGTTACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCt                                                                       >  1:2529146/1‑93 (MQ=255)
                   ccGGTTACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCtg                                                                      >  2:3893598/1‑93 (MQ=255)
                    cGGTTACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCtga                                                                     <  2:3565413/93‑1 (MQ=255)
                    cGGTTACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCtga                                                                     <  2:1647937/93‑1 (MQ=255)
                     ggTTACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCtgat                                                                    >  2:3984296/1‑93 (MQ=255)
                      gTTACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCtgatg                                                                   <  1:1465217/93‑1 (MQ=255)
                      gTTACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCtgatg                                                                   <  2:4469377/93‑1 (MQ=255)
                      gTTACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCtgatg                                                                   >  1:1924077/1‑93 (MQ=255)
                       ttACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCtgatga                                                                  >  1:1040592/1‑93 (MQ=255)
                       ttACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCtgatga                                                                  >  1:3658929/1‑93 (MQ=255)
                       ttACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCtgatga                                                                  <  1:2665264/93‑1 (MQ=255)
                        tACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCtgatgag                                                                 >  1:2623154/1‑92 (MQ=255)
                        tACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGAc                                                                 >  2:4544662/1‑93 (MQ=255)
                           cAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGAt                                                              <  1:4533976/93‑1 (MQ=255)
                           cAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGAt                                                              <  1:3457743/93‑1 (MQ=255)
                            aGCGAACGGGTGGTACGGCGAATATTGCAGCGGTGAACGTCGCCCTCCGCGGATTTCACAACCGCGTGGCCACCCAAACCGCTGATGACGaaa                                                             >  2:138268/1‑91 (MQ=255)
                            aGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATa                                                             <  1:989274/93‑1 (MQ=255)
                              gcaaCGGATGGGACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATAcc                                                           <  2:2669869/91‑1 (MQ=255)
                               gAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCt                                                          <  2:461845/93‑1 (MQ=255)
                               gAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCt                                                          >  1:3577155/1‑93 (MQ=255)
                                aaCGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCtt                                                         >  1:4505940/1‑93 (MQ=255)
                                  cGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTtca                                                       <  2:1263656/93‑1 (MQ=255)
                                  cGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTtca                                                       <  2:870876/93‑1 (MQ=255)
                                    gATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTtcatc                                                     >  1:3365132/1‑93 (MQ=255)
                                    gATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTtcatc                                                     >  1:4575218/1‑93 (MQ=255)
                                    gATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTtcatc                                                     <  2:3517182/93‑1 (MQ=255)
                                     aTGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTtcatca                                                    <  2:3766289/93‑1 (MQ=255)
                                     aTGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTtcatca                                                    <  1:1311587/93‑1 (MQ=255)
                                     aTGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTtcatca                                                    <  2:2171856/93‑1 (MQ=255)
                                        ggaCGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGc                                                 <  1:961572/91‑1 (MQ=255)
                                        gTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGc                                                 <  2:1431133/93‑1 (MQ=255)
                                          aCGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTc                                               >  2:885895/1‑93 (MQ=255)
                                                 atatTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAAc                                        >  2:3027524/1‑93 (MQ=255)
                                                  tatTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACa                                       <  2:3019671/93‑1 (MQ=255)
                                                   atTGCAGCGGTGATCGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAg                                      <  2:4374639/93‑1 (MQ=255)
                                                    ttGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCCGCGTGCATACCAAAGCGGCTCATGACGATACCTTCATCAGGCTCGCCAAACAGa                                     >  1:765927/1‑93 (MQ=255)
                                                      gCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGAtt                                   <  2:2331153/93‑1 (MQ=255)
                                                      gCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGAtt                                   <  1:1847486/93‑1 (MQ=255)
                                                      gCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGAtt                                   <  1:2132986/93‑1 (MQ=255)
                                                         gCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTTGCCAAAAAGATTgtc                                >  1:1640599/1‑93 (MQ=255)
                                                         gCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTgtc                                <  1:1133010/93‑1 (MQ=255)
                                                         gCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTgtc                                <  1:2196243/93‑1 (MQ=255)
                                                           ggTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTgtcgt                              <  2:1246282/93‑1 (MQ=255)
                                                               aaCGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTa                          >  1:1010945/1‑93 (MQ=255)
                                                                aCGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAg                         <  1:3877287/93‑1 (MQ=255)
                                                                 cGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGt                        >  2:1926397/1‑93 (MQ=255)
                                                                 cGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGt                        <  1:583948/93‑1 (MQ=255)
                                                                  gTCGCCATCGGCGGATTCCTCATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGGCGTAGTc                       >  2:4541573/1‑93 (MQ=255)
                                                                  gTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTc                       >  2:2832911/1‑93 (MQ=255)
                                                                   tCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCg                      >  1:4040878/1‑93 (MQ=255)
                                                                    cGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCgg                     <  1:1743869/93‑1 (MQ=255)
                                                                    cGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCgg                     >  1:3943960/1‑93 (MQ=255)
                                                                     gCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCggg                    >  2:177913/1‑93 (MQ=255)
                                                                       cATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCGGGCt                  <  1:3998222/93‑1 (MQ=255)
                                                                        aTCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCGGGCtt                 >  1:839388/1‑93 (MQ=255)
                                                                        aTCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCGGGCtt                 <  2:3597882/93‑1 (MQ=255)
                                                                        aTCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCGGGCtt                 >  2:1060676/1‑93 (MQ=255)
                                                                            gcggATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTAGTCGTAGTCGGGCTTCTcc             >  2:1972288/1‑93 (MQ=255)
                                                                              ggATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCGGGCTTCTCCtt           >  2:712601/1‑93 (MQ=255)
                                                                               gATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCGGGGTTCTCCTTa          >  2:2764555/1‑93 (MQ=255)
                                                                               gATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCGGGCTTCTCCTTa          <  1:975787/93‑1 (MQ=255)
                                                                               gATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCGGGCTTCTCCTTa          >  2:2495330/1‑93 (MQ=255)
                                                                               gATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCGGGCTTCTCCTTa          <  2:1696234/93‑1 (MQ=255)
                                                                                 ttCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCGGGCTTCTCCTTAGa        >  1:871212/1‑93 (MQ=255)
                                                                                  tCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCGGGCTTCTCCTTAGAc       <  1:3157930/93‑1 (MQ=255)
                                                                                  tCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCGGGCTTCTCCTTAGAc       >  1:2338412/1‑93 (MQ=255)
                                                                                  tCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCGGGCTTCTCCTTAGAc       <  1:1392943/93‑1 (MQ=255)
                                                                                  tCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCGGGCTTCTCCTTAGAc       >  2:3363468/1‑93 (MQ=255)
                                                                                    cacaTCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCGGGCTTCTCCTTAGACGt     <  2:2279089/93‑1 (MQ=255)
                                                                                       aTCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCGGGCTTCTCCTTAGACGtttt  <  1:4386267/93‑1 (MQ=255)
                                                                                       aTCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCGGGCTTCTCCTTAGACGtttt  >  2:3438657/1‑93 (MQ=255)
                                                                                          |                                                                                         
ACGCCAGACTACGCGGTCGCCGGTTACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCAGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCGGGCTTCTCCTTAGACGTTTT  >  NC_000913/4388842‑4389021

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CGGACGCCAGACTACGCGGTCGCCGGTTACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCAGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCGGGCTTCTCCTTAGACGTTTTAAGA  >  NC_000913/4388839‑4389025
                                                                                             |                                                                                             
CGGACGCCAGACTACGCGGTCGCCGGTTACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGCGAACGTCGCCATCGGCGGATTCCACATCG                                                                                               >  SRR4302535.3251463/1‑94 (MQ=60)
   ACGCCAGACTACGCGGTCGCCGGTTACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCG                                                                                            >  SRR4302535.4185932/1‑94 (MQ=60)
     GCCAGACTACGCGGTCGCCGGTTACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTG                                                                                          <  SRR4302535.256885/94‑1 (MQ=60)
     GCCAGACTACGCGGTCGCCGGTTACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTG                                                                                          <  SRR4302535.2038464/94‑1 (MQ=60)
         GACTACGCGGTCGCCGGTTACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATC                                                                                      >  SRR4302535.2821436/1‑94 (MQ=60)
         GACTACGCGGTCGCCGGTTACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATA                                                                                      <  SRR4302535.517464/94‑1 (MQ=60)
           CTACGCGGTCGCCGGTTACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGTTTCCACATCCGAGTGCATACC                                                                                    >  SRR4302535.4391185/1‑94 (MQ=60)
           CTACGCGGTCGCCGGTTACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACC                                                                                    <  SRR4302535.3116834/94‑1 (MQ=60)
           CTACGCGGTCGCCGGTTACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACC                                                                                    >  SRR4302535.4174932/1‑94 (MQ=60)
           CTACGCGGTCGCCGGTTACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACC                                                                                    >  SRR4302535.4307121/1‑94 (MQ=60)
            TACGCGGTCGCCGGTTACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCA                                                                                   <  SRR4302535.3806644/94‑1 (MQ=60)
              CGCGGTCGCCGGTTACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAA                                                                                 <  SRR4302535.1308124/94‑1 (MQ=60)
              CGCGGTCGCCGGTTACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAA                                                                                 <  SRR4302535.3042903/94‑1 (MQ=60)
               GCGGTCGCCGGTTACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAG                                                                                >  SRR4302535.1635672/1‑94 (MQ=60)
               GCGGTCGCCGGTTACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAG                                                                                <  SRR4302535.2018826/94‑1 (MQ=60)
                 GGTCGCCGGTTACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCG                                                                              >  SRR4302535.2858431/1‑94 (MQ=60)
                 GGTCGCCGGTTACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCG                                                                              <  SRR4302535.4393826/94‑1 (MQ=60)
                    CGCCGGTTACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCT                                                                           >  SRR4302535.2547368/1‑94 (MQ=60)
                    CGCCGGTTACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCT                                                                           <  SRR4302535.919931/94‑1 (MQ=60)
                      CCGGTTACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGA                                                                         <  SRR4302535.556945/94‑1 (MQ=60)
                      CCGGTTACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATAGGCGGATTCCACATCGGCGTGCATACCAAAGCTGCTGA                                                                         >  SRR4302535.4066357/1‑94 (MQ=60)
                       CGGTTACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGAT                                                                        <  SRR4302535.4556963/94‑1 (MQ=60)
                       CGGTTACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGAT                                                                        >  SRR4302535.410242/1‑94 (MQ=60)
                       CGGTTACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCAATCGGCGGATTCCCCATCGGCGCGCATACCAAAGCGGCTGAG                                                                        >  SRR4302535.2193005/1‑94 (MQ=60)
                        GGTTACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATG                                                                       <  SRR4302535.2005967/94‑1 (MQ=60)
                        GGTTACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATG                                                                       >  SRR4302535.1937945/1‑94 (MQ=60)
                         gtttccagggaaccggatgttacgccaaatttggagcggttaaccgCGCCAACGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGA                                                                      <  SRR4302535.3313109/48‑1 (MQ=60)
                         GTTACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGA                                                                      <  SRR4302535.365203/94‑1 (MQ=60)
                         GTTACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGA                                                                      >  SRR4302535.3684256/1‑94 (MQ=60)
                         GTTACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGA                                                                      <  SRR4302535.1086285/94‑1 (MQ=60)
                         GTTACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGA                                                                      >  SRR4302535.1047972/1‑94 (MQ=60)
                         GTTACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGA                                                                      <  SRR4302535.1475714/94‑1 (MQ=60)
                          TTACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGAG                                                                     >  SRR4302535.2641953/1‑94 (MQ=60)
                          TTACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGAC                                                                     <  SRR4302535.3640418/94‑1 (MQ=60)
                          TTACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGAC                                                                     <  SRR4302535.2041823/94‑1 (MQ=60)
                          TTACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGAC                                                                     <  SRR4302535.2684348/94‑1 (MQ=60)
                             CCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGAT                                                                  >  SRR4302535.1198315/1‑94 (MQ=60)
                              CAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATA                                                                 <  SRR4302535.3481783/94‑1 (MQ=60)
                              CAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATA                                                                 <  SRR4302535.4565004/94‑1 (MQ=60)
                              CAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCCCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATA                                                                 <  SRR4302535.978329/94‑1 (MQ=60)
                               AGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATAC                                                                <  SRR4302535.996299/94‑1 (MQ=60)
                                 CGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCT                                                              <  SRR4302535.98735/94‑1 (MQ=60)
                                 CGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCT                                                              >  SRR4302535.163843/1‑94 (MQ=60)
                                 CGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCT                                                              <  SRR4302535.4438122/94‑1 (MQ=60)
                                 CGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCT                                                              >  SRR4302535.3601954/1‑94 (MQ=60)
                                 AGAACGGAGGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCT                                                              <  SRR4302535.2002871/94‑1 (MQ=60)
                                  GAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTT                                                             <  SRR4302535.2680625/94‑1 (MQ=60)
                                  GAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTT                                                             <  SRR4302535.1676669/94‑1 (MQ=60)
                                  GAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTT                                                             >  SRR4302535.4536871/1‑94 (MQ=60)
                                  GAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTT                                                             <  SRR4302535.1314349/94‑1 (MQ=60)
                                    ACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCA                                                           >  SRR4302535.3073275/1‑94 (MQ=60)
                                      GGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATC                                                         >  SRR4302535.4606398/1‑94 (MQ=60)
                                      GGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATC                                                         <  SRR4302535.3972115/94‑1 (MQ=60)
                                      GGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATC                                                         >  SRR4302535.3388549/1‑94 (MQ=60)
                                        ATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAG                                                       <  SRR4302535.4615638/94‑1 (MQ=60)
                                        ATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAG                                                       <  SRR4302535.1320960/94‑1 (MQ=60)
                                           GGACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCT                                                    <  SRR4302535.968405/94‑1 (MQ=60)
                                             ACGGCGAATATTGCAGCTGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCG                                                  <  SRR4302535.376611/94‑1 (MQ=60)
                                                 CGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAA                                              >  SRR4302535.2382763/1‑94 (MQ=60)
                                                 CGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAA                                              >  SRR4302535.311129/1‑94 (MQ=60)
                                                  GAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAA                                             >  SRR4302535.1450928/1‑94 (MQ=60)
                                                   AATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAAC                                            >  SRR4302535.1813950/1‑94 (MQ=60)
                                                     TATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAG                                          <  SRR4302535.4574136/94‑1 (MQ=60)
                                                      ATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCCGCGTGCATACCAAAGCGGCTCATGACGATACCTTCATCAGGCTCGCCAAACAGA                                         >  SRR4302535.771319/1‑94 (MQ=60)
                                                        TGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATT                                       >  SRR4302535.867466/1‑94 (MQ=60)
                                                         GCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTG                                      <  SRR4302535.1860799/94‑1 (MQ=60)
                                                         GCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTG                                      <  SRR4302535.2148360/94‑1 (MQ=60)
                                                          CAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGT                                     <  SRR4302535.3882312/94‑1 (MQ=60)
                                                           AGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTTGCCAAAAAGATTGTC                                    >  SRR4302535.1652458/1‑94 (MQ=60)
                                                           AGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTC                                    >  SRR4302535.2038166/1‑94 (MQ=60)
                                                            GCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCG                                   <  SRR4302535.3499081/94‑1 (MQ=60)
                                                            GCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCG                                   <  SRR4302535.2211895/94‑1 (MQ=60)
                                                            GCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCG                                   <  SRR4302535.1141098/94‑1 (MQ=60)
                                                             CGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGT                                  <  SRR4302535.2299679/94‑1 (MQ=60)
                                                              GGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTC                                 <  SRR4302535.1399399/94‑1 (MQ=60)
                                                              GGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTC                                 <  SRR4302535.3957130/94‑1 (MQ=60)
                                                                 GAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTA                              >  SRR4302535.1018127/1‑94 (MQ=60)
                                                                   ACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTGCCCAAACAGATTGTCGTCGTAGG                            >  SRR4302535.253618/1‑94 (MQ=60)
                                                                   ACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGT                            <  SRR4302535.3903913/94‑1 (MQ=60)
                                                                    CGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTC                           <  SRR4302535.588043/94‑1 (MQ=60)
                                                                    CGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTC                           <  SRR4302535.4380444/94‑1 (MQ=60)
                                                                     GTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCG                          <  SRR4302535.796261/94‑1 (MQ=60)
                                                                     GTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCG                          <  SRR4302535.1880436/94‑1 (MQ=60)
                                                                     GTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCG                          >  SRR4302535.2548089/1‑94 (MQ=60)
                                                                     GTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCG                          >  SRR4302535.4068541/1‑94 (MQ=60)
                                                                      TCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCGG                         >  SRR4302535.3970889/1‑94 (MQ=60)
                                                                       CGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACCGCTTGACGTCGTAGTCGGG                        >  SRR4302535.3138536/1‑94 (MQ=60)
                                                                       CGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCGGG                        >  SRR4302535.2791001/1‑94 (MQ=60)
                                                                       CGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCGGG                        >  SRR4302535.768683/1‑94 (MQ=60)
                                                                       CGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCGGG                        <  SRR4302535.1756486/94‑1 (MQ=60)
                                                                         CCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCGGGCT                      <  SRR4302535.3441163/94‑1 (MQ=60)
                                                                          CATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCGGGCTT                     >  SRR4302535.845283/1‑94 (MQ=60)
                                                                          CATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCGGGCTT                     <  SRR4302535.2602296/94‑1 (MQ=60)
                                                                          CATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCGGGCTT                     >  SRR4302535.1030775/1‑94 (MQ=60)
                                                                          CATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCGGGCTT                     <  SRR4302535.4025577/94‑1 (MQ=60)
                                                                               GCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCGGGCTTCTCCT                <  SRR4302535.4259835/94‑1 (MQ=60)
                                                                                 GGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCGGGCTTCTCCTTA              >  SRR4302535.1614860/1‑94 (MQ=60)
                                                                                  GATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCGGGCTTCTCCTTAG             <  SRR4302535.982712/94‑1 (MQ=60)
                                                                                   ATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCGGGCTTCTCCTTAGA            >  SRR4302535.877386/1‑94 (MQ=60)
                                                                                    TTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCGGGCTTCTCCTTAGAC           >  SRR4302535.2355145/1‑94 (MQ=60)
                                                                                     TCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCGGGCTTCTCCTTAGACG          <  SRR4302535.4450646/94‑1 (MQ=60)
                                                                                     TCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCGGGCTTCTCCTTAGACG          <  SRR4302535.3179850/94‑1 (MQ=60)
                                                                                     TCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCGGGCTTCTCCTTAGACG          <  SRR4302535.1402889/94‑1 (MQ=60)
                                                                                       CACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCGGGCTTCTCCTTAGACGTT        >  SRR4302535.4224735/1‑94 (MQ=60)
                                                                                        ACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTAGTAGTCGGGCTTCTCCTTAGACGTTT       <  SRR4302535.1444809/94‑1 (MQ=60)
                                                                                         CATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCGGGCTTCTCCTTAGACGTTTT      <  SRR4302535.811562/94‑1 (MQ=60)
                                                                                          ATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCGGGCTTCTCCTTAGACGTTTTA     <  SRR4302535.4416275/94‑1 (MQ=60)
                                                                                          ATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCGGGCTTCTCCTTAGACGTTTTA     >  SRR4302535.2836672/1‑94 (MQ=60)
                                                                                            CGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCGGGCTTCTCCTTAGGCGTTTTAAG   >  SRR4302535.2685189/1‑94 (MQ=60)
                                                                                             GGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCGGGCTTCTCCTTAGACGTTTTAAGA  <  SRR4302535.4536811/94‑1 (MQ=60)
                                                                                             GGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCGGGCTTCTCCTTAGACGTTTTAAGA  >  SRR4302535.4234140/1‑94 (MQ=60)
                                                                                             GGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGATCGCCAAACAGATTGTCGTCGTAGTCGGGCTTCTCCTTAGACGTTTTAAGA  >  SRR4302535.158388/1‑94 (MQ=60)
                                                                                             |                                                                                             
CGGACGCCAGACTACGCGGTCGCCGGTTACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCAGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCGGGCTTCTCCTTAGACGTTTTAAGA  >  NC_000913/4388839‑4389025

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑