Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1873	183.1	9376148	98.2%	9207377	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,428,092	A→G	G217G (GGA→GGG)	cycA →	transport of D‑alanine, D‑serine, and glycine

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,428,092	0	A	G	100.0%	537.6 / NA	169	G217G (GGA→GGG)	cycA	transport of D‑alanine, D‑serine, and glycine
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (62/107); total (62/107)

Only 100 of 181 total aligned reads displayed.

TCAGTCACCGACTGGTGTGGAAGCGTCATTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGATTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCAAAGATCCAGAGAAATCACTGCCACG  >  NC_000913/4428002‑4428181

tCAGTCACCGACTGGTGTGGAAGCGTCATTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGtt                                                                                         >  1:18613/1‑93 (MQ=255)

 cAGTCACCGACTGGTGTGGAAGCGTCATTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTc                                                                                        <  2:3961243/93‑1 (MQ=255)

   gTCACCGACTGGTGTGGAAGCGTCATTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTTCa                                                                                      >  2:114219/1‑93 (MQ=255)

   gTCACCGACTGGTGTGGAAGCGTCATTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCa                                                                                      <  1:3018672/93‑1 (MQ=255)

     cACCGACTGGTGTGGAAGCGTCATTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGa                                                                                    <  1:4341922/93‑1 (MQ=255)

      aCCGACTGGTGTGGAAGCGTCATTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGAt                                                                                   <  2:1011480/93‑1 (MQ=255)

      aCCGACTGGTGTGGAAGCGTCATTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGAt                                                                                   <  1:1933358/93‑1 (MQ=255)

      aCCGACTGGTGTGGAAGCGTCATTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGAt                                                                                   <  2:174491/93‑1 (MQ=255)

      aCCGACTGGTGTGGAAGCGTCATTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCCTTGCCGGGGTCCAgct                                                                                   >  2:1916001/1‑91 (MQ=255)

       ccGACTGGTGTGGAAGCGTCATTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATa                                                                                  <  1:3862882/93‑1 (MQ=255)

       ccGACTGGTGTGGAAGCGTCATTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATa                                                                                  <  2:2330582/93‑1 (MQ=255)

       ccGACTGGTGTGGAAGCGTCATTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATa                                                                                  <  2:1536769/93‑1 (MQ=255)

         gACTGGTGTGGAAGCGTCATTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAgg                                                                                >  1:3684393/1‑92 (MQ=255)

         gACTGGTGTGGAAGCGTCATTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGc                                                                                <  1:2586490/93‑1 (MQ=255)

         gACTGGTGGGGAAGCGTCATTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGc                                                                                <  2:817668/93‑1 (MQ=255)

          aCTGGTGTGGAAGCGTCATTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCg                                                                               <  1:2522196/93‑1 (MQ=255)

          aCTGGTGTGGAAGCGTCATTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCg                                                                               >  1:4521732/1‑93 (MQ=255)

           cTGGTGTGGAAGCGTCATTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCgg                                                                              <  2:2309354/93‑1 (MQ=255)

           cTGGTGTGGAAGCGTCATTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCgg                                                                              <  2:3614551/93‑1 (MQ=255)

            tGGTGTGGAAGCGTCATTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGt                                                                             <  2:2413753/93‑1 (MQ=255)

            tGGTGTGGAAGCGTCATTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGt                                                                             >  1:696079/1‑93 (MQ=255)

             ggTGTGGAAGCGTCATTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGtt                                                                            >  2:3670652/1‑93 (MQ=255)

             ggTGTGGAAGCGTCATTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGtt                                                                            <  1:3786612/93‑1 (MQ=255)

               tgtgGAAGCGTCATTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGtttt                                                                          <  2:3143531/93‑1 (MQ=255)

                    aaGCGTCATTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCtt                                                                     <  1:780319/93‑1 (MQ=255)

                     aGCGTCATTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCttt                                                                    <  1:661394/93‑1 (MQ=255)

                     aGCGTCATTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCttt                                                                    >  1:4006219/1‑93 (MQ=255)

                      gCGTCATTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTc                                                                   <  1:797886/93‑1 (MQ=255)

                      gCGTCATTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTc                                                                   <  1:2606381/93‑1 (MQ=255)

                      gCGTCATTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTc                                                                   >  1:3362431/1‑93 (MQ=255)

                        gTCATTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCTCTTTCGt                                                                 <  2:2905964/93‑1 (MQ=255)

                        ctgaTTCGCGCATTTGTGGAATGACGGCGTCTGCTTCCAGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGt                                                                 <  1:3185451/90‑1 (MQ=255)

                         tCATTCGCGCATTTGTGGAATGACGTCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTg                                                                <  1:813997/93‑1 (MQ=255)

                         tCATTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTg                                                                <  1:4107970/93‑1 (MQ=255)

                         tCATTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTg                                                                <  1:2840400/93‑1 (MQ=255)

                         tCATTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTg                                                                <  2:4467571/93‑1 (MQ=255)

                          cATTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTgg                                                               <  1:3384613/93‑1 (MQ=255)

                          cATTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTgg                                                               <  1:3565305/93‑1 (MQ=255)

                           aTTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTggg                                                              <  2:4322444/93‑1 (MQ=255)

                            ttCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTgggg                                                             >  2:728993/1‑93 (MQ=255)

                             tCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGa                                                            <  1:848850/93‑1 (MQ=255)

                             tCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGa                                                            <  2:2778290/93‑1 (MQ=255)

                               gcgcATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGAtt                                                          >  2:3996386/1‑93 (MQ=255)

                               gcgcATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGCTCCAGATAGCGGATTTCGCTTTCGTGGGGAtt                                                          >  1:753378/1‑93 (MQ=255)

                                cgcATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTg                                                         <  2:1529789/93‑1 (MQ=255)

                                 gcATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGa                                                        <  1:4550317/93‑1 (MQ=255)

                                   aTTTGTGGAATGACGGCGGCTGGTTCCCGGAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGGTTTCGCTTTCGTGGGGATTGAGc                                                      >  1:1674639/1‑93 (MQ=255)

                                   aTTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGc                                                      >  1:2199734/1‑93 (MQ=255)

                                   aTTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGGTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGc                                                      >  2:2610909/1‑93 (MQ=255)

                                    tttGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCt                                                     >  2:3509114/1‑93 (MQ=255)

                                    tttGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCt                                                     >  2:3637284/1‑93 (MQ=255)

                                     ttGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTg                                                    <  1:4051536/93‑1 (MQ=255)

                                        tgGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTggta                                                 >  2:3426489/1‑93 (MQ=255)

                                         ggAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTggtag                                                >  2:1035805/1‑93 (MQ=255)

                                            aTGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGC‑TTCGTGGGGATTGAGCTGGTAGGTac                                            >  1:4596503/1‑93 (MQ=255)

                                               gcggcggCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTacaa                                          <  2:2661339/92‑1 (MQ=255)

                                                cggcggCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTacaac                                         <  1:2362259/93‑1 (MQ=255)

                                                cggaggCTGGTTCCCGAAAGGTATAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTacaac                                         <  2:4681691/93‑1 (MQ=255)

                                                 ggcggcTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTacaaca                                        <  2:764365/93‑1 (MQ=255)

                                                 ggcggcTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTacaaca                                        <  2:2506428/93‑1 (MQ=255)

                                                   gggcTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGc                                      <  2:2144903/92‑1 (MQ=255)

                                                   cggcTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGGCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGc                                      <  1:2324148/93‑1 (MQ=255)

                                                   cggcTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGc                                      <  1:1551480/93‑1 (MQ=255)

                                                     gcTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTg                                    >  1:4064412/1‑93 (MQ=255)

                                                      cTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGc                                   <  2:3773843/93‑1 (MQ=255)

                                                      cTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGc                                   <  1:626195/93‑1 (MQ=255)

                                                       tGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCg                                  >  1:289414/1‑93 (MQ=255)

                                                           tCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGaac                              >  1:3187341/1‑92 (MQ=255)

                                                           tCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGaaa                              <  2:3339445/93‑1 (MQ=255)

                                                             ccGAAAGGTTTAAGTGGCTTCTTTTCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAAcc                            >  1:1394845/1‑93 (MQ=255)

                                                                aaaGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCaac                         <  1:4424557/93‑2 (MQ=255)

                                                                aaaGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCaaa                         >  1:3840538/1‑93 (MQ=255)

                                                                aaaGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCaaa                         <  2:3146363/93‑1 (MQ=255)

                                                                  agtgttAAGTGGCTTCTTTGCAGCATTCCAGATAGCGGTTTTCGCCTTCGTGAGGATTGAACTGGTAGATACAGCAGCTGCAGAAACCAAAGa                       <  2:4189380/89‑1 (MQ=255)

                                                                  aGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCAAAGa                       <  1:370629/93‑1 (MQ=255)

                                                                  aGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCAAAGa                       <  2:1301176/93‑1 (MQ=255)

                                                                  aGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCAAAGa                       <  1:737909/93‑1 (MQ=255)

                                                                  aGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCAAAGa                       <  1:1113946/93‑1 (MQ=255)

                                                                  aGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCAAAGa                       <  1:250220/93‑1 (MQ=255)

                                                                    gTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCAAAGATc                     >  1:3887036/1‑93 (MQ=255)

                                                                      ttAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCAAAGATCCa                   <  1:3464093/93‑1 (MQ=255)

                                                                      ttAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCAAAGATCCa                   <  2:1477127/93‑1 (MQ=255)

                                                                      ttAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCAAAGATCCa                   <  2:1236148/93‑1 (MQ=255)

                                                                      ttAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTATCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCAAAGATCCa                   <  2:3032504/93‑1 (MQ=255)

                                                                        aaGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCAAAGATCCaga                 <  1:1592743/93‑1 (MQ=255)

                                                                        aaGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCAAAGATCCaga                 <  2:4090064/93‑1 (MQ=255)

                                                                         aGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCAAAGATCCagag                >  1:871452/1‑93 (MQ=255)

                                                                          gTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCAAAGATCCAgaga               <  1:3573955/93‑1 (MQ=255)

                                                                           tGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCAAAGATCCAGAGaa              >  2:2453100/1‑93 (MQ=255)

                                                                               ttcttTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCAAAGATCCAGAGAAATCa          <  1:1274186/93‑1 (MQ=255)

                                                                                tcttTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCAAAGATCCAGAGAAATCAc         >  2:3831466/1‑93 (MQ=255)

                                                                                tcttTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCAAAGATCCAGAGAAATCAc         <  1:3943095/93‑1 (MQ=255)

                                                                                tcttTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCAAAGATCCAGAGAAATCAc         >  1:1198807/1‑93 (MQ=255)

                                                                                tcttTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCAAAGATCCAGAGAAATCAc         >  1:2767918/1‑93 (MQ=255)

                                                                                tcttTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCAAAGATCCAGAGAAATCAc         <  2:2058349/93‑1 (MQ=255)

                                                                                tcttTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCAAAGATCCAGAGAAATCAc         <  1:2309060/93‑1 (MQ=255)

                                                                                  tttGCTGGGTTCCAGATAGCCGTTTTCGATTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCAAAGATCCAGAGAAAGCACTg       <  2:2322659/93‑1 (MQ=255)

                                                                                   ttGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCAAAGATCCAGAGAAATCACTGc      <  2:1666886/93‑1 (MQ=255)

                                                                                     gCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCAAAGATCCAGAGAAAGCACTGCCa    <  2:425403/93‑1 (MQ=255)

                                                                                       cGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCAAAGATCCAGAGAAATCACTGCCAcg  <  2:4404350/93‑1 (MQ=255)

TCAGTCACCGACTGGTGTGGAAGCGTCATTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGATTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCAAAGATCCAGAGAAATCACTGCCACG  >  NC_000913/4428002‑4428181

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

TTCAGTCACCGACTGGTGTGGAAGCGTCATTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGATTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCAAAGATCCAGAGAAATCACTGCCACGCG  >  NC_000913/4428001‑4428183

TTCAGTCACCGACTGGTGTGGAAGCGTCATTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTT                                                                                           >  SRR4302535.18751/1‑94 (MQ=60)

    GTCACCGACTGGTGTGGAAGCGTCATTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAG                                                                                       <  SRR4302535.3039817/94‑1 (MQ=60)

      CACCGACTGGTGTGGAAGCGTCATTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGAT                                                                                     <  SRR4302535.4371617/94‑1 (MQ=60)

      CACCGACTGGTGTGGAAGCGTCATTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGAT                                                                                     <  SRR4302535.2730093/94‑1 (MQ=60)

       ACCGACTGGTGTGGAAGCGTCATTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATA                                                                                    <  SRR4302535.1947282/94‑1 (MQ=60)

       ACCGACTGGTGTGGAAGCGTCATTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGCTCCAGATA                                                                                    >  SRR4302535.3722165/1‑94 (MQ=60)

        CCGACTGGTGTGGAAGCGTCATTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAG                                                                                   <  SRR4302535.3889458/94‑1 (MQ=60)

        CCGACTGGTGTGGAAGCGTCATTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAG                                                                                   <  SRR4302535.3819871/94‑1 (MQ=60)

         CGACTGGTGTGGAAGCGTCATTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGG                                                                                  >  SRR4302535.3709885/1‑94 (MQ=60)

          GACTGGTGTGGAAGCGTCATTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCG                                                                                 >  SRR4302535.4552720/1‑94 (MQ=60)

          GACTGGTGTGGAAGCGTCATTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCG                                                                                 <  SRR4302535.2605065/94‑1 (MQ=60)

          GACTGGTGTGGAAGCGTCATTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGCTCCAGATAGCG                                                                                 >  SRR4302535.3104294/1‑94 (MQ=60)

           ACTGGTGTGGAAGCGTCATTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGG                                                                                <  SRR4302535.2540371/94‑1 (MQ=60)

            CTGGTGTGGAAGCGTCATTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGT                                                                               <  SRR4302535.3925514/94‑1 (MQ=60)

            CTGGTGTGGAAGCGTCATTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGT                                                                               >  SRR4302535.700960/1‑94 (MQ=60)

              GGTGTGGAAGCGTCATTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTT                                                                             <  SRR4302535.3812732/94‑1 (MQ=60)

                TGTGGAAGCGTCATTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTTTTTGCCCGGTTCCAGATAGCGGTTTTC                                                                           >  SRR4302535.3468401/1‑94 (MQ=60)

                 GTGGAAGCGTCATTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCG                                                                          >  SRR4302535.4120552/1‑94 (MQ=60)

                 GTGGAAGCGTCATTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCG                                                                          <  SRR4302535.737457/94‑1 (MQ=60)

                   GGAAGCGTCATTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGGTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCT                                                                        >  SRR4302535.1287671/1‑94 (MQ=60)

                    GAAGCGTCATTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTT                                                                       <  SRR4302535.1219166/94‑1 (MQ=60)

                     AAGCGTCATTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTT                                                                      <  SRR4302535.785796/94‑1 (MQ=60)

                     AAGCGTCATTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTT                                                                      >  SRR4302535.4033622/1‑94 (MQ=60)

                     AAGCGTCATTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGACGGGTTCCCGATAGCGGTTTTCGCTTT                                                                      >  SRR4302535.3761815/1‑94 (MQ=60)

                      AGCGTCATTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTC                                                                     >  SRR4302535.3385828/1‑94 (MQ=60)

                      AGCGTCATTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTC                                                                     <  SRR4302535.666008/94‑1 (MQ=60)

                      AGCGTCATTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTC                                                                     >  SRR4302535.365694/1‑94 (MQ=60)

                      AGCGTCATTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTC                                                                     >  SRR4302535.843375/1‑94 (MQ=60)

                       GCGTCATTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCG                                                                    <  SRR4302535.2625085/94‑1 (MQ=60)

                       GCGTCATTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCG                                                                    <  SRR4302535.803486/94‑1 (MQ=60)

                         ctgATTCGCGCATTTGTGGAATGACGGCGTCTGCTTCCAGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTG                                                                  <  SRR4302535.3207475/91‑1 (MQ=60)

                         GTCATTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTG                                                                  >  SRR4302535.3578562/1‑94 (MQ=60)

                          TCATTCGCGCATTTGTGGAATGACGTCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGG                                                                 <  SRR4302535.819707/94‑1 (MQ=60)

                          TCATTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGG                                                                 <  SRR4302535.4136097/94‑1 (MQ=60)

                          TCATTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGG                                                                 <  SRR4302535.2860550/94‑1 (MQ=60)

                          GCATTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGG                                                                 <  SRR4302535.1417279/94‑1 (MQ=60)

                           CATTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGG                                                                <  SRR4302535.3408164/94‑1 (MQ=60)

                           CATTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGG                                                                <  SRR4302535.3590028/94‑1 (MQ=60)

                              TCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGAT                                                             <  SRR4302535.854820/94‑1 (MQ=60)

                              TCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGAGGTTTTCGCTTTCGTGGGGAT                                                             >  SRR4302535.771551/1‑94 (MQ=60)

                               CGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGCTCCAGATAGCGGATTTCGCTTTCGTGGGGATT                                                            >  SRR4302535.758681/1‑94 (MQ=60)

                                  GCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAG                                                         <  SRR4302535.4581420/94‑1 (MQ=60)

                                   CATTTGTGGAATGACGGCGGCTGGTTCCCGGAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGGTTTCGCTTTCGTGGGGATTGAGC                                                        >  SRR4302535.1686764/1‑94 (MQ=60)

                                   CATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGC                                                        >  SRR4302535.2999019/1‑94 (MQ=60)

                                   CATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGC                                                        >  SRR4302535.2215411/1‑94 (MQ=60)

                                   CATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGC                                                        <  SRR4302535.192438/94‑1 (MQ=60)

                                    ATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCT                                                       <  SRR4302535.457898/94‑1 (MQ=60)

                                     TTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTG                                                      <  SRR4302535.814103/94‑1 (MQ=60)

                                      TTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGG                                                     <  SRR4302535.4079273/94‑1 (MQ=60)

                                      TTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGG                                                     <  SRR4302535.4409997/94‑1 (MQ=60)

                                      TTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGG                                                     <  SRR4302535.2514086/94‑1 (MQ=60)

                                           GAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGT                                                >  SRR4302535.650139/1‑94 (MQ=60)

                                            AATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGC‑TTCGTGGGGATTGAGCTGGTAGGTAC                                              >  SRR4302535.4627776/1‑94 (MQ=60)

                                            AATGACGGCGGCTGGTTCCCGAAAGGTTTAAGCGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTA                                               >  SRR4302535.1138023/1‑94 (MQ=60)

                                             ATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTAC                                              >  SRR4302535.1705302/1‑94 (MQ=60)

                                                ACGGAGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAAC                                           <  SRR4302535.332295/94‑1 (MQ=60)

                                                 CGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACA                                          <  SRR4302535.2379224/94‑1 (MQ=60)

                                                 CGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACA                                          <  SRR4302535.2346009/94‑1 (MQ=60)

                                                  GGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAG                                         <  SRR4302535.2277817/94‑1 (MQ=60)

                                                    CGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGGCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCT                                       <  SRR4302535.2340778/94‑1 (MQ=60)

                                                    CGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCT                                       <  SRR4302535.4498432/94‑1 (MQ=60)

                                                    CGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCT                                       <  SRR4302535.1562651/94‑1 (MQ=60)

                                                     GGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTG                                      >  SRR4302535.286334/1‑94 (MQ=60)

                                                     GGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTG                                      >  SRR4302535.4092225/1‑94 (MQ=60)

                                                       CTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCG                                    >  SRR4302535.291439/1‑94 (MQ=60)

                                                       CTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCG                                    <  SRR4302535.630552/94‑1 (MQ=60)

                                                       CTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCG                                    >  SRR4302535.925058/1‑94 (MQ=60)

                                                           TTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAC                                >  SRR4302535.3209384/1‑94 (MQ=60)

                                                            TCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAAC                               <  SRR4302535.4031238/94‑1 (MQ=60)

                                                             CCCGAAAGGTTTAAGTGGCTTCTTTTCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACC                              >  SRR4302535.1404805/1‑94 (MQ=60)

                                                               CGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCAA                            >  SRR4302535.793992/1‑94 (MQ=60)

                                                                GAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCAAA                           <  SRR4302535.2540678/94‑1 (MQ=60)

                                                                GAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCAAA                           >  SRR4302535.3867002/1‑94 (MQ=60)

                                                                 AAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCAACG                          <  SRR4302535.4454861/94‑1 (MQ=60)

                                                                   AGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCAAAGAT                        <  SRR4302535.251943/94‑1 (MQ=60)

                                                                   AGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCAAAGAT                        <  SRR4302535.743096/94‑1 (MQ=60)

                                                                   AGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCAAAGAT                        >  SRR4302535.1967333/1‑94 (MQ=60)

                                                                   AGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCAAAGAT                        <  SRR4302535.1121888/94‑1 (MQ=60)

                                                                   AGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCAAAGAT                        <  SRR4302535.373191/94‑1 (MQ=60)

                                                                    GGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCAAAGATC                       >  SRR4302535.3913697/1‑94 (MQ=60)

                                                                       TTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCAAAGATCCAG                    >  SRR4302535.4326082/1‑94 (MQ=60)

                                                                       TTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCAAAGATCCAG                    <  SRR4302535.3488171/94‑1 (MQ=60)

                                                                         AAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCAAAGATCCAGAG                  >  SRR4302535.877628/1‑94 (MQ=60)

                                                                         AAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCAAAGATCCAGAG                  <  SRR4302535.1604238/94‑1 (MQ=60)

                                                                           GTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCAAAGATCCAGAGAA                <  SRR4302535.3598735/94‑1 (MQ=60)

                                                                             GGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCAAAGATCCAGAGAAAT              <  SRR4302535.2521694/94‑1 (MQ=60)

                                                                             GGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCAAAGATCCAGAGAAAT              <  SRR4302535.690930/94‑1 (MQ=60)

                                                                               CTTCTTTGCCGGGTTCCAGATAGCAGTTATCGCTTTAGTGAGGATTGAACTAGTAGGAACAACAGCTGCGGAAACCAAAGATCCAGAGAAATCA            <  SRR4302535.4710117/94‑1 (MQ=60)

                                                                                TTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCAAAGATCCAGAGAAATCAC           <  SRR4302535.1567156/94‑1 (MQ=60)

                                                                                TTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCAAAGATCCAGAGAAATCAC           <  SRR4302535.1283282/94‑1 (MQ=60)

                                                                                TTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCAAAGATCCAGAGAAATCAC           >  SRR4302535.1207396/1‑94 (MQ=60)

                                                                                TTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCAAAGATCCAGAGAAATCAC           >  SRR4302535.2787626/1‑94 (MQ=60)

                                                                                 TCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCAAAGATCCAGAGAAATCACT          <  SRR4302535.3970013/94‑1 (MQ=60)

                                                                                 TCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCAAAGATCCAGAGAAATCACT          <  SRR4302535.2325589/94‑1 (MQ=60)

                                                                                  CTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCAAAGATCCAGAGAAATCACTG         >  SRR4302535.3897257/1‑94 (MQ=60)

                                                                                   TTTGCCGGGTTGCAGATAGCGTTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCAAAGATCCAGAGAAATCACTGC        <  SRR4302535.2795625/94‑1 (MQ=60)

                                                                                    TTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTCCAACAGCTGCGGAAACCAAAGATCCAGAGAAATCACTGCC       <  SRR4302535.3408065/94‑1 (MQ=60)

                                                                                      GCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCAAAGATCCAGAGAAATCACTGCCAC     >  SRR4302535.40495/1‑94 (MQ=60)

                                                                                        CGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCAAAGATCCAGAGAAATCACTGCCACGC   >  SRR4302535.4254667/1‑94 (MQ=60)

                                                                                         GGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCAAAGATCCAGAGAAATCACTGCCACGCG  >  SRR4302535.658474/1‑94 (MQ=60)

TTCAGTCACCGACTGGTGTGGAAGCGTCATTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGATTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCAAAGATCCAGAGAAATCACTGCCACGCG  >  NC_000913/4428001‑4428183

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 5 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`