Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1873 183.1 9376148 98.2% 9207377 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,435,689 C→T D135D (GAC→GAT)  ytfI → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,435,6890CT99.5% 723.3 / ‑5.4 197D135D (GAC→GAT) ytfIhypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base T (105/91);  total (105/92)
Fisher's exact test for biased strand distribution p-value = 4.67e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.08e-01

Only 100 of 201 total aligned reads displayed.

TTGAATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAAC‑AAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCGATTGGTGACGTG  >  NC_000913/4435601‑4435778
                                                                                         |                                                                                         
ttGAATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAgatgat                                                                                        <  1:382109/93‑1 (MQ=255)
  gAATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTg                                                                                      <  1:3967912/93‑1 (MQ=255)
   aaTACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGt                                                                                     >  1:3900141/1‑93 (MQ=255)
    aTACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTa                                                                                    <  2:812363/93‑1 (MQ=255)
    aTACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTa                                                                                    <  2:376413/93‑1 (MQ=255)
     tACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTAt                                                                                   >  2:2353866/1‑93 (MQ=255)
     aaCGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTAt                                                                                   <  2:1733602/92‑1 (MQ=255)
      aCGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATg                                                                                  >  1:3594038/1‑93 (MQ=255)
      aCGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTGCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATg                                                                                  <  1:2014035/93‑1 (MQ=255)
          agaaATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTg                                                                              <  1:389049/93‑1 (MQ=255)
          agaaATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTg                                                                              <  1:1285825/93‑1 (MQ=255)
           gaaATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTgg                                                                             >  2:4318708/1‑93 (MQ=255)
            aaaTACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTggg                                                                            >  2:1110019/1‑93 (MQ=255)
              aTACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGat                                                                          <  1:1731885/93‑1 (MQ=255)
               tACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGtta                                                                         <  2:2935695/93‑1 (MQ=255)
                aCCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGatat                                                                        >  1:2361379/1‑93 (MQ=255)
                 cccATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATAtt                                                                       >  1:3094186/1‑93 (MQ=255)
                 cccATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATAtt                                                                       >  1:1505065/1‑93 (MQ=255)
                 cccATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATAtt                                                                       <  2:1493561/93‑1 (MQ=255)
                  ccATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTc                                                                      >  1:251035/1‑93 (MQ=255)
                  ccATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTc                                                                      >  2:4289001/1‑93 (MQ=255)
                   cATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATAttat                                                                     >  1:3697354/1‑91 (MQ=255)
                   cATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCt                                                                     <  2:656858/93‑1 (MQ=255)
                     tAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTAc                                                                   >  1:2681605/1‑93 (MQ=255)
                     tAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTAc                                                                   >  2:247388/1‑93 (MQ=255)
                      aaCAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACa                                                                  <  2:4216899/93‑1 (MQ=255)
                       aCAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAg                                                                 >  1:1500340/1‑93 (MQ=255)
                           tAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGaaaa                                                             >  2:1802319/1‑93 (MQ=255)
                           tAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGaaaa                                                             >  1:1027078/1‑93 (MQ=255)
                           tAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGaaaa                                                             >  1:3494979/1‑93 (MQ=255)
                           tAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGaaaa                                                             >  2:3202509/1‑93 (MQ=255)
                             aaTGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGaaaaaa                                                           <  2:4615635/93‑1 (MQ=255)
                              aTGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAAc                                                          >  1:439065/1‑93 (MQ=255)
                              aTGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAAc                                                          <  1:2644880/93‑1 (MQ=255)
                               tGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACa                                                         >  2:2629792/1‑93 (MQ=255)
                               tGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACa                                                         <  1:2126373/93‑1 (MQ=255)
                                  atatAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTc                                                      >  2:2678517/1‑93 (MQ=255)
                                  atatAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTc                                                      <  2:1413008/93‑1 (MQ=255)
                                  atatAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTc                                                      <  2:578112/93‑1 (MQ=255)
                                   tataACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCt                                                     <  1:1889585/93‑1 (MQ=255)
                                   tataACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCt                                                     <  1:804017/93‑1 (MQ=255)
                                   tataACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCt                                                     >  1:1883720/1‑93 (MQ=255)
                                   tataACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCt                                                     >  2:3295694/1‑93 (MQ=255)
                                     taACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGa                                                   >  1:2033687/1‑93 (MQ=255)
                                       aCTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACa                                                 <  2:284264/93‑1 (MQ=255)
                                       aCTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACa                                                 >  1:294536/1‑93 (MQ=255)
                                        cTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACAt                                                >  1:1563096/1‑93 (MQ=255)
                                          cattcatGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATAc                                              >  2:7917/1‑93 (MQ=255)
                                           attcatGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATAcc                                             <  2:4125525/93‑1 (MQ=255)
                                            ttcatGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCg                                            >  1:521869/1‑93 (MQ=255)
                                            ttcatGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCg                                            >  1:1313742/1‑93 (MQ=255)
                                            ttcatGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCg                                            >  1:1358475/1‑93 (MQ=255)
                                             tcatGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGa                                           >  2:1911394/1‑93 (MQ=255)
                                              catGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAATCTAACATACCGaa                                          >  2:3055162/1‑93 (MQ=255)
                                               atGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGaaa                                         >  2:2788810/1‑93 (MQ=255)
                                               atGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGaaa                                         >  1:4062030/1‑93 (MQ=255)
                                                tGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGaaaa                                        <  2:4413295/93‑1 (MQ=255)
                                                  gATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAgg                                      >  1:4642839/1‑93 (MQ=255)
                                                   aTTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGa                                     >  1:3203886/1‑93 (MQ=255)
                                                   aTTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGa                                     >  2:1301530/1‑93 (MQ=255)
                                                    tttCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGAt                                    <  2:2247964/93‑1 (MQ=255)
                                                     ttCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGAtt                                   <  2:1815564/93‑1 (MQ=255)
                                                     ttCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGAtt                                   <  2:2064156/93‑1 (MQ=255)
                                                     ttCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGAtt                                   <  1:112656/93‑1 (MQ=255)
                                                      tCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTa                                  <  1:4257709/93‑1 (MQ=255)
                                                       cAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTaa                                 <  2:510773/93‑1 (MQ=255)
                                                       cAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTaa                                 <  2:708729/93‑1 (MQ=255)
                                                        aaaaaCCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTaaa                                <  1:286816/93‑1 (MQ=255)
                                                         aaaaCCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAg                               <  2:296939/93‑1 (MQ=255)
                                                         aaaaCCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAg                               >  2:1131394/1‑93 (MQ=255)
                                                           aaCCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGt                             >  2:3965290/1‑93 (MQ=255)
                                                            aCCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGtt                            >  1:4468401/1‑93 (MQ=255)
                                                             ccGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTta                           <  1:3850917/93‑1 (MQ=255)
                                                             ccGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTta                           <  1:2958287/93‑1 (MQ=255)
                                                              cGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTtat                          >  2:3486918/1‑93 (MQ=255)
                                                               gTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTtata                         <  1:2578754/93‑1 (MQ=255)
                                                                tGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTatat                        >  1:3380917/1‑93 (MQ=255)
                                                                tGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTatat                        >  1:3230337/1‑93 (MQ=255)
                                                                  aaTTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTc                      <  1:2660420/93‑1 (MQ=255)
                                                                    tttCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCAc                    >  2:2106225/1‑93 (MQ=255)
                                                                    tttCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCAc                    >  2:4497207/1‑93 (MQ=255)
                                                                    tttCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCAc                    <  1:1627014/93‑1 (MQ=255)
                                                                     ttCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCAcc                   <  1:3708480/93‑1 (MQ=255)
                                                                        cGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAgg                <  2:4060682/93‑1 (MQ=255)
                                                                         gTTAGAACAAAACAGATGATTTTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGc               <  1:1932344/93‑1 (MQ=255)
                                                                         gTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGc               >  1:2773544/1‑93 (MQ=255)
                                                                         gTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGc               >  2:4373424/1‑93 (MQ=255)
                                                                          ttAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCg              >  1:3021248/1‑93 (MQ=255)
                                                                          ttAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCg              <  2:2288043/93‑1 (MQ=255)
                                                                            aGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCgct            >  2:2817197/1‑91 (MQ=255)
                                                                               acaaaacaGATGATTGTATGCCTGGGTTATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCGATTgg         <  1:572835/90‑1 (MQ=255)
                                                                               acaaaacaGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCGATTgg         >  1:513890/4‑93 (MQ=255)
                                                                               acaaaacaGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCGATTgg         <  1:788375/90‑1 (MQ=255)
                                                                                caaaacaGATGATTGTATGCCTTGGATATTCTACAGAAAAAACAGTCTGACATCCCGAAAAGGATTAAAGGTTATATTCACCAGGCGATTGGt        <  2:454034/91‑1 (MQ=255)
                                                                                 aaaacaGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCGATTGGTg       >  1:393067/2‑93 (MQ=255)
                                                                                 aaaacaGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCGATTGGTg       >  2:630450/2‑93 (MQ=255)
                                                                                  aaacaGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCGATTGGTGa      <  1:2437978/93‑1 (MQ=255)
                                                                                   aacaGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCGATTGGTGAc     >  1:1283776/1‑93 (MQ=255)
                                                                                     caGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCGATTGGTGACgt   <  2:4034508/93‑1 (MQ=255)
                                                                                      aGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCGATTGGTGACgtg  >  1:1158603/1‑93 (MQ=255)
                                                                                         |                                                                                         
TTGAATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAAC‑AAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCGATTGGTGACGTG  >  NC_000913/4435601‑4435778

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TTTGAATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAAC‑AAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCGATTGGTGACGTGTCTT  >  NC_000913/4435600‑4435782
                                                                                          |                                                                                             
TTTGAATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGAT                                                                                            <  SRR4302535.2866488/94‑1 (MQ=60)
 TTGAATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATT                                                                                           <  SRR4302535.384753/94‑1 (MQ=60)
 TTGAATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATT                                                                                           <  SRR4302535.3962337/94‑1 (MQ=60)
   GAATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGT                                                                                         >  SRR4302535.3875541/1‑94 (MQ=60)
   GAATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGT                                                                                         >  SRR4302535.3926856/1‑94 (MQ=60)
   GAATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGT                                                                                         <  SRR4302535.3995014/94‑1 (MQ=60)
      TACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATG                                                                                      >  SRR4302535.3618980/1‑94 (MQ=60)
       ACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGC                                                                                     >  SRR4302535.756044/1‑94 (MQ=60)
       ACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTGCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGC                                                                                     <  SRR4302535.2028543/94‑1 (MQ=60)
        CGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCT                                                                                    >  SRR4302535.3956525/1‑94 (MQ=60)
        CGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCC                                                                                    >  SRR4302535.4125831/1‑94 (MQ=60)
           AGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGG                                                                                 <  SRR4302535.1294989/94‑1 (MQ=60)
           AGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGG                                                                                 <  SRR4302535.391753/94‑1 (MQ=60)
            GAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGG                                                                                >  SRR4302535.285531/1‑94 (MQ=60)
               ATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATA                                                                             <  SRR4302535.1744404/94‑1 (MQ=60)
                TACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATAT                                                                            <  SRR4302535.2047275/94‑1 (MQ=60)
                TACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATAT                                                                            >  SRR4302535.2378334/1‑94 (MQ=60)
                 ACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATT                                                                           >  SRR4302535.1515885/1‑94 (MQ=60)
                 ACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATT                                                                           >  SRR4302535.3115733/1‑94 (MQ=60)
                  CCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTC                                                                          >  SRR4302535.252763/1‑94 (MQ=60)
                  CCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTC                                                                          >  SRR4302535.2910359/1‑94 (MQ=60)
                   CCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCT                                                                         >  SRR4302535.3722942/1‑94 (MQ=60)
                   CCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTAT                                                                         >  SRR4302535.3722940/1‑94 (MQ=60)
                     ATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTAC                                                                       >  SRR4302535.2700789/1‑94 (MQ=60)
                       AACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAG                                                                     >  SRR4302535.1511134/1‑94 (MQ=60)
                         CAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAA                                                                   >  SRR4302535.3237194/1‑94 (MQ=60)
                           ATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAA                                                                 >  SRR4302535.3519259/1‑94 (MQ=60)
                           ATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAA                                                                 >  SRR4302535.1034371/1‑94 (MQ=60)
                           ATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAA                                                                 >  SRR4302535.3168915/1‑94 (MQ=60)
                             AAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAA                                                               >  SRR4302535.143233/1‑94 (MQ=60)
                              AATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAAC                                                              >  SRR4302535.442138/1‑94 (MQ=60)
                              AATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAAC                                                              <  SRR4302535.2563376/94‑1 (MQ=60)
                               ATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACA                                                             <  SRR4302535.2663823/94‑1 (MQ=60)
                                TGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAG                                                            <  SRR4302535.2141701/94‑1 (MQ=60)
                                TGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAG                                                            >  SRR4302535.3453888/1‑94 (MQ=60)
                                  AATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTC                                                          <  SRR4302535.1047654/94‑1 (MQ=60)
                                   ATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCT                                                         >  SRR4302535.266922/1‑94 (MQ=60)
                                   ATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCT                                                         >  SRR4302535.1897297/1‑94 (MQ=60)
                                   ATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCT                                                         >  SRR4302535.3276764/1‑94 (MQ=60)
                                   ATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCT                                                         >  SRR4302535.248176/1‑94 (MQ=60)
                                    TATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTG                                                        <  SRR4302535.2979865/94‑1 (MQ=60)
                                    TATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTG                                                        <  SRR4302535.4085550/94‑1 (MQ=60)
                                    TATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTG                                                        <  SRR4302535.809662/94‑1 (MQ=60)
                                    TATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTG                                                        <  SRR4302535.1514842/94‑1 (MQ=60)
                                    TATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTG                                                        <  SRR4302535.1903196/94‑1 (MQ=60)
                                     ATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGA                                                       >  SRR4302535.2048340/1‑94 (MQ=60)
                                     ATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGA                                                       <  SRR4302535.2690809/94‑1 (MQ=60)
                                      TAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGAC                                                      >  SRR4302535.2025148/1‑94 (MQ=60)
                                       AACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACA                                                     <  SRR4302535.3113584/94‑1 (MQ=60)
                                       AACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACA                                                     >  SRR4302535.563811/1‑94 (MQ=60)
                                       AACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACA                                                     >  SRR4302535.296592/1‑94 (MQ=60)
                                        ACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACAT                                                    >  SRR4302535.1574357/1‑94 (MQ=60)
                                         CTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATA                                                   >  SRR4302535.3808174/1‑94 (MQ=60)
                                         CTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATA                                                   >  SRR4302535.2126557/1‑94 (MQ=60)
                                           CATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACC                                                 <  SRR4302535.3701404/94‑1 (MQ=60)
                                            ATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCG                                                >  SRR4302535.1323131/1‑94 (MQ=60)
                                            ATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCG                                                >  SRR4302535.1888255/1‑94 (MQ=60)
                                            ATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCG                                                >  SRR4302535.1368190/1‑94 (MQ=60)
                                            ATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCG                                                >  SRR4302535.525520/1‑94 (MQ=60)
                                               CATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAA                                             >  SRR4302535.4089832/1‑94 (MQ=60)
                                                 TGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAG                                           <  SRR4302535.3534339/94‑1 (MQ=60)
                                                  GGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGG                                          >  SRR4302535.4674308/1‑94 (MQ=60)
                                                  GGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGG                                          <  SRR4302535.1227696/94‑1 (MQ=60)
                                                   GATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGA                                         >  SRR4302535.3226074/1‑94 (MQ=60)
                                                    ATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGAT                                        >  SRR4302535.1321455/1‑94 (MQ=60)
                                                      TTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTA                                      <  SRR4302535.113447/94‑1 (MQ=60)
                                                      TTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTA                                      <  SRR4302535.1142763/94‑1 (MQ=60)
                                                       TCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAA                                     >  SRR4302535.1587648/1‑94 (MQ=60)
                                                       TCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAA                                     <  SRR4302535.4286877/94‑1 (MQ=60)
                                                         AAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAG                                   >  SRR4302535.2975175/1‑94 (MQ=60)
                                                         AAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAG                                   <  SRR4302535.288823/94‑1 (MQ=60)
                                                         AAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAG                                   <  SRR4302535.517714/94‑1 (MQ=60)
                                                          AAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGG                                  <  SRR4302535.3901901/94‑1 (MQ=60)
                                                          AAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGG                                  >  SRR4302535.1696541/1‑94 (MQ=60)
                                                          AAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGG                                  <  SRR4302535.1902392/94‑1 (MQ=60)
                                                          AAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGG                                  >  SRR4302535.2598976/1‑94 (MQ=60)
                                                           AAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGT                                 <  SRR4302535.1516325/94‑1 (MQ=60)
                                                            AACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTT                                >  SRR4302535.4499061/1‑94 (MQ=60)
                                                              CCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTAT                              <  SRR4302535.3877416/94‑1 (MQ=60)
                                                              CCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTAT                              <  SRR4302535.2451827/94‑1 (MQ=60)
                                                              CCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTAT                              <  SRR4302535.2979062/94‑1 (MQ=60)
                                                               CGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATA                             <  SRR4302535.215536/94‑1 (MQ=60)
                                                               CGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATA                             <  SRR4302535.4704894/94‑1 (MQ=60)
                                                                GTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATAT                            <  SRR4302535.2597282/94‑1 (MQ=60)
                                                                GTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATAT                            >  SRR4302535.3404420/1‑94 (MQ=60)
                                                                GTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATAT                            >  SRR4302535.3252731/1‑94 (MQ=60)
                                                                GTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATAT                            <  SRR4302535.3921444/94‑1 (MQ=60)
                                                                   AATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCA                         <  SRR4302535.2679478/94‑1 (MQ=60)
                                                                     TTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACC                       <  SRR4302535.1638759/94‑1 (MQ=60)
                                                                      TTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCA                      <  SRR4302535.3734158/94‑1 (MQ=60)
                                                                         CGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGC                   <  SRR4302535.1353260/94‑1 (MQ=60)
                                                                         CGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGC                   >  SRR4302535.2793293/1‑94 (MQ=60)
                                                                         CGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGC                   >  SRR4302535.4359320/1‑94 (MQ=60)
                                                                          GTTAGAACAAAACAGATGATTTTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCG                  <  SRR4302535.1946266/94‑1 (MQ=60)
                                                                          GTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCG                  >  SRR4302535.3042405/1‑94 (MQ=60)
                                                                             AGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCGATT               >  SRR4302535.110497/1‑94 (MQ=60)
                                                                             AGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCGATT               >  SRR4302535.1397693/1‑94 (MQ=60)
                                                                             AGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCGATT               >  SRR4302535.3768038/1‑94 (MQ=60)
                                                                               AACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCGATTGG             >  SRR4302535.517479/1‑94 (MQ=60)
                                                                                acaAAACAGATGATTGTATGCCTGGGTTATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCGATTGGT            <  SRR4302535.576857/91‑1 (MQ=60)
                                                                                acaAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCGATTGGT            <  SRR4302535.793911/91‑1 (MQ=60)
                                                                                 caAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCGATTGGTG           <  SRR4302535.1293384/92‑1 (MQ=60)
                                                                                 caAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCGATTGGTG           >  SRR4302535.395805/3‑94 (MQ=60)
                                                                                 A‑AAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCGATTGGTGA          >  SRR4302535.4500764/1‑94 (MQ=60)
                                                                                   AAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCGATTGGTGAC         >  SRR4302535.1292927/1‑94 (MQ=60)
                                                                                   AAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCGATTGGTGAC         <  SRR4302535.2455539/94‑1 (MQ=60)
                                                                                   AAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCGATTGGTGAC         <  SRR4302535.2774077/94‑1 (MQ=60)
                                                                                     ACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCGATTGGTGACGT       <  SRR4302535.4466142/94‑1 (MQ=60)
                                                                                      CAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCGATTGGTGACGTG      <  SRR4302535.1919203/94‑1 (MQ=60)
                                                                                      CAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCGATTGGTGACGTG      >  SRR4302535.79988/1‑94 (MQ=60)
                                                                                      CAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCGATTGGTGACGTG      >  SRR4302535.1166886/1‑94 (MQ=60)
                                                                                       AGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCGATTGGTGACGTGT     >  SRR4302535.2260397/1‑94 (MQ=60)
                                                                                       AGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCGATTGGTGACGTGT     >  SRR4302535.1690261/1‑94 (MQ=60)
                                                                                       AGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCGATTGGTGACGTGT     >  SRR4302535.1561309/1‑94 (MQ=60)
                                                                                         ATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCGATTGGTGACGTGTCT   <  SRR4302535.3797033/94‑1 (MQ=60)
                                                                                         ATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCGATTGGTGACGTGTCT   >  SRR4302535.4307002/1‑94 (MQ=60)
                                                                                          TGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCGATTGGTGACGTGTCTT  >  SRR4302535.3435893/1‑94 (MQ=60)
                                                                                          |                                                                                             
TTTGAATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAAC‑AAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCGATTGGTGACGTGTCTT  >  NC_000913/4435600‑4435782

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑