Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1873 183.1 9376148 98.2% 9207377 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,437,861 A→G V311A (GTG→GCG)  ytfL ← putative transport protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,437,8610AG100.0% 627.7 / NA 205V311A (GTG→GCG) ytfLputative transport protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (99/106);  total (99/106)

Only 100 of 215 total aligned reads displayed.

AGCCCCTGACCGACCAGATCGCCCATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCACCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCACAATCAGCGTGTTGCGAATTTGT  >  NC_000913/4437771‑4437950
                                                                                          |                                                                                         
 gCCCCTGACCGACCAGATCGCCCATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCcg                                                                                        <  1:4322389/93‑1 (MQ=255)
   cccTGACCGACCAGATCGCCCATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATcgccgca                                                                                      >  1:2861193/1‑92 (MQ=255)
   cccTGACCGACCAGATCGCCCATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCcgcg                                                                                      >  2:4638438/1‑93 (MQ=255)
     cTGACCGACCAGATCGCCCATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGaa                                                                                    <  1:2926497/93‑1 (MQ=255)
     cTGACCGACCAGATCGCCCATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGaa                                                                                    <  2:2704732/93‑1 (MQ=255)
      tgtccgaccAGATCGCCCATCAGCGTGGTCATCACGTCATTGAGGTTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAg                                                                                   <  2:4652051/90‑1 (MQ=255)
       gaccgaccAGATCGCCCATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGt                                                                                  >  2:170170/1‑93 (MQ=255)
       gaccgaccAGATCGCCCATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGt                                                                                  >  1:1714355/1‑93 (MQ=255)
       gaccgaccAGATCGCCCATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGt                                                                                  >  2:809605/1‑93 (MQ=255)
        accgaccAGATCGCCCATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTc                                                                                 >  1:4496077/1‑93 (MQ=255)
        accgaccAGATCGCCCATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTc                                                                                 <  1:1466671/93‑1 (MQ=255)
         ccgaccAGATCGCCCATCAGCGTGGTCATCACGTCATTGAGGGGGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCt                                                                                >  1:78926/1‑93 (MQ=255)
          cgaccAGATCGCCCATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCtt                                                                               <  1:4554860/93‑1 (MQ=255)
          cgaccAGATCGCCCATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCtt                                                                               <  2:1896197/93‑1 (MQ=255)
           gaccAGATCGCCCATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTc                                                                              >  2:3043360/1‑93 (MQ=255)
           gaccAGATCCCCCATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTc                                                                              <  1:696774/93‑1 (MQ=255)
            accAGATCGCCCATCAGCGTGGTCATTACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCa                                                                             >  1:123615/1‑93 (MQ=255)
            accAGATCGCCCATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCa                                                                             <  2:4218435/93‑1 (MQ=255)
             ccAGATCGCCCATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGACGTCTTCAc                                                                            >  2:1104698/1‑93 (MQ=255)
             ccAGATCGCCCATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCAc                                                                            >  1:1810433/1‑93 (MQ=255)
             ccAGATCGCCCATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCAc                                                                            <  1:1551688/93‑1 (MQ=255)
              cAGATCGCCCATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCAcc                                                                           <  1:4666808/93‑1 (MQ=255)
              cAGATCGCCCATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCAcc                                                                           <  2:1840684/93‑1 (MQ=255)
               aGATCGCCCATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCt                                                                          >  1:3732743/1‑93 (MQ=255)
               aGATCGCCCATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCt                                                                          >  2:1481644/1‑93 (MQ=255)
               aGATCGCCCATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCt                                                                          >  2:4345070/1‑93 (MQ=255)
                gATCGCCCATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTg                                                                         >  2:120261/1‑93 (MQ=255)
                 aTCGCCCATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGc                                                                        <  2:3164262/93‑1 (MQ=255)
                    gCCCATCAGCGTGTTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGt                                                                     <  1:3476474/93‑1 (MQ=255)
                    gCCCATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGt                                                                     >  1:2654495/1‑93 (MQ=255)
                      ccATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGttt                                                                   >  2:470075/1‑93 (MQ=255)
                       cATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGtttt                                                                  <  2:2217831/93‑1 (MQ=255)
                       aaTCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGttta                                                                  >  1:550976/2‑92 (MQ=255)
                         tCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTaa                                                                >  1:1218201/1‑93 (MQ=255)
                         tCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTaa                                                                >  2:2527513/1‑93 (MQ=255)
                         tCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTaa                                                                >  1:2281945/1‑93 (MQ=255)
                          cAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTaaa                                                               >  2:1554759/1‑93 (MQ=255)
                          cAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTaaa                                                               <  2:3341079/93‑1 (MQ=255)
                             cGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACt                                                            <  1:3999573/93‑1 (MQ=255)
                             cGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACt                                                            <  2:3998042/93‑1 (MQ=255)
                              gTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACtt                                                           >  2:3585754/1‑93 (MQ=255)
                              gTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACtt                                                           <  1:970738/93‑1 (MQ=255)
                               tGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCTGCGAAGTCTTCACCTGCGGTTTTAAAACttt                                                          <  2:3087746/93‑1 (MQ=255)
                                 gTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTcc                                                        <  1:3509532/93‑1 (MQ=255)
                                 gTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTcc                                                        >  1:3346359/1‑93 (MQ=255)
                                 gTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTcc                                                        <  2:234310/93‑1 (MQ=255)
                                   catcaCGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCaa                                                      <  2:278067/93‑1 (MQ=255)
                                     tcaCGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACg                                                    >  2:2391940/1‑93 (MQ=255)
                                     tcaCGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACg                                                    >  2:3712937/1‑93 (MQ=255)
                                     tcaCGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACg                                                    >  1:3097632/1‑93 (MQ=255)
                                       aCGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGcc                                                  >  1:4256637/1‑93 (MQ=255)
                                       aCGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGcc                                                  >  1:4456584/1‑93 (MQ=255)
                                       aCGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGcc                                                  <  1:1609367/93‑1 (MQ=255)
                                          tCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCctct                                               >  2:2815856/1‑93 (MQ=255)
                                            aTTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGTGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGa                                             <  1:3305059/93‑1 (MQ=255)
                                              tGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGaaa                                           <  1:2110084/93‑1 (MQ=255)
                                               gAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAg                                          <  2:4001091/93‑1 (MQ=255)
                                               gAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAg                                          <  2:668605/93‑1 (MQ=255)
                                                aGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAgg                                         <  2:284276/93‑1 (MQ=255)
                                                  ggTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGt                                       <  2:2490237/93‑1 (MQ=255)
                                                  ggTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGt                                       <  1:3044138/93‑1 (MQ=255)
                                                       tgatCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACg                                  <  1:2514506/93‑1 (MQ=255)
                                                         atCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTa                                <  1:3135859/93‑1 (MQ=255)
                                                         atCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTa                                >  2:244849/1‑93 (MQ=255)
                                                           ccccACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATc                              >  2:1044423/1‑93 (MQ=255)
                                                           ccccACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATc                              <  1:2789617/93‑1 (MQ=255)
                                                                ccaccaGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGca                         <  1:1139971/93‑1 (MQ=255)
                                                                ccaccaGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGca                         <  1:2742879/93‑1 (MQ=255)
                                                                ccaccaGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGca                         >  1:3271181/1‑93 (MQ=255)
                                                                 caccaGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGcac                        <  1:1018539/93‑1 (MQ=255)
                                                                   ccaGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCACaa                      >  1:1188717/1‑93 (MQ=255)
                                                                   ccaGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCACaa                      >  1:4038810/1‑93 (MQ=255)
                                                                      gcgcGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCACAATCa                   <  1:2310583/93‑1 (MQ=255)
                                                                       cgcgTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCACAATCAg                  <  2:4512501/93‑1 (MQ=255)
                                                                        gcgTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCACCGCCTCTGAAAGGGTTAACGTATCCGGCACAATCAGc                 <  2:1263707/93‑1 (MQ=255)
                                                                        gcgTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCACAATCAGc                 <  2:4117938/93‑1 (MQ=255)
                                                                         cgTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCACAATCAGCg                <  2:3462939/93‑1 (MQ=255)
                                                                         cgTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCACAATCAGCg                >  2:1487141/1‑93 (MQ=255)
                                                                          gTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCACAATCAGCgt               <  2:1713900/93‑1 (MQ=255)
                                                                          gTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCACAATCAGCgt               >  2:3246809/1‑93 (MQ=255)
                                                                          gTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCACAATCAGCgt               <  1:1045216/93‑1 (MQ=255)
                                                                          gTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCACAATCAGCgt               <  2:3365050/93‑1 (MQ=255)
                                                                          gTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCACAATCAGCgt               >  1:411279/1‑93 (MQ=255)
                                                                          gTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCACAATCAGCgg               >  1:1389658/1‑92 (MQ=255)
                                                                           tACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCACAATCAGCgtg              >  2:2600979/1‑93 (MQ=255)
                                                                            aCTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCACAATCAGCgtgt             >  2:193834/1‑93 (MQ=255)
                                                                             cTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCACAATCAGCGTGtt            <  2:2314582/93‑1 (MQ=255)
                                                                               cGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCACAATCAGCGTGTTGc          <  2:94992/93‑1 (MQ=255)
                                                                               cGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCACAATCAGCGTGTTGc          <  1:3942376/93‑1 (MQ=255)
                                                                               cGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCACAATCAGCGTGTTGc          <  1:2021229/93‑1 (MQ=255)
                                                                                  tCATGAGCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCACAATCAGCGTGTTGCGaa       <  2:3385944/93‑1 (MQ=255)
                                                                                    aTGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCACAATCAGCGTGTTGCGAAtt     >  1:980272/1‑93 (MQ=255)
                                                                                    aTGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCACAATCAGCGTGTTGCGAAtt     <  1:904707/93‑1 (MQ=255)
                                                                                     tGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCACAATCAGCGTGTTGCGAAttt    >  1:4606965/1‑93 (MQ=255)
                                                                                     tGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCACAATCAGCGTGTTGCGAAttt    >  1:3792221/1‑93 (MQ=255)
                                                                                     tGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCACAATCAGCGTGTTGCGAAttt    >  1:3603609/1‑93 (MQ=255)
                                                                                     tGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCACAATCAGCGTGTTGCGAAttt    >  1:3450117/1‑93 (MQ=255)
                                                                                     tGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCACAATCAGCGTGTTGCGAAttt    >  1:2580976/1‑93 (MQ=255)
                                                                                      gATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCACAATCAGCGTGTTGCGAATTTg   <  2:1447990/93‑1 (MQ=255)
                                                                                      gATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCACAATCAGCGTGTTGCGAATTTg   <  1:1272152/93‑1 (MQ=255)
                                                                                          |                                                                                         
AGCCCCTGACCGACCAGATCGCCCATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCACCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCACAATCAGCGTGTTGCGAATTTGT  >  NC_000913/4437771‑4437950

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CCAGCCCCTGACCGACCAGATCGCCCATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCACCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCACAATCAGCGTGTTGCGAATTTGTACG  >  NC_000913/4437769‑4437953
                                                                                            |                                                                                            
CCAGCCCCTGACCGACCAGATCGCCCATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGC                                                                                             >  SRR4302535.2931445/1‑94 (MQ=60)
 CAGCCCCTGACCGACCAGATCGCCCATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCC                                                                                            <  SRR4302535.1682005/94‑1 (MQ=60)
  AGCCCCTGACCGCCCAGCTCGCCCATCCTCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCCCCAGCGCGTACTCGTTCATGATCGCCG                                                                                           <  SRR4302535.1212432/94‑1 (MQ=60)
  AGCCCCTGACCGACCAGATCGCCCATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCG                                                                                           >  SRR4302535.823603/1‑94 (MQ=60)
   GCCCCTGACCGACCAGATCGCCCATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGC                                                                                          >  SRR4302535.995094/1‑94 (MQ=60)
   GCCCCTGACCGACCAGATCGCCCATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGC                                                                                          <  SRR4302535.4351958/94‑1 (MQ=60)
    CCCCTGACCTACCAGATAGCCCATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCG                                                                                         <  SRR4302535.3705707/94‑1 (MQ=60)
    CCCCTGACCGACCAGATCGCCCATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCG                                                                                         >  SRR4302535.1428700/1‑94 (MQ=60)
    CCCCTGACCGACCAGATCGCCCATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCG                                                                                         <  SRR4302535.2122605/94‑1 (MQ=60)
    CCCCTGACCGACCAGATCGCCCATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCA                                                                                         >  SRR4302535.2881461/1‑94 (MQ=60)
       CTGACCGACCAGATCGCCCATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAG                                                                                      <  SRR4302535.2947126/94‑1 (MQ=60)
       CTGACCGACCAGATCGCCCATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAG                                                                                      <  SRR4302535.523776/94‑1 (MQ=60)
        TGACCGACCAGATCGCCCATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGT                                                                                     >  SRR4302535.4104198/1‑94 (MQ=60)
        TGACCGACCAGATCGCCCATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGT                                                                                     >  SRR4302535.1726752/1‑94 (MQ=60)
         GACCGACCAGATCGCCCATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTC                                                                                    <  SRR4302535.3579536/94‑1 (MQ=60)
         GACCGACCAGATCGCCCATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTC                                                                                    <  SRR4302535.3350041/94‑1 (MQ=60)
         GACCGACCAGATCGCCCATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTC                                                                                    >  SRR4302535.4526943/1‑94 (MQ=60)
         GACCGACCAGATCGCCCATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTC                                                                                    <  SRR4302535.3151943/94‑1 (MQ=60)
          ACCGACCAGATCGCCCATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCT                                                                                   >  SRR4302535.3203782/1‑94 (MQ=60)
          ACCGACCAGATCGCCCATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCT                                                                                   <  SRR4302535.1477179/94‑1 (MQ=60)
          ACCGACCAGATCGCCCATCAGCGTGGTCATCACGTCATTGAGGGGGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCT                                                                                   >  SRR4302535.79491/1‑94 (MQ=60)
           CCGACCAGATCGCCCATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTT                                                                                  <  SRR4302535.3490814/94‑1 (MQ=60)
           CCGACCAGATCGCCCATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTT                                                                                  <  SRR4302535.2549343/94‑1 (MQ=60)
           CCGACCAGATCGCCCATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTT                                                                                  <  SRR4302535.4539535/94‑1 (MQ=60)
            CGACCAGATCGCCCATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTC                                                                                 <  SRR4302535.4585973/94‑1 (MQ=60)
             GACCAGATCGCCCATCAGCGTGGTCATTACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCA                                                                                >  SRR4302535.124482/1‑94 (MQ=60)
             GACCAGATCGCCCATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCA                                                                                <  SRR4302535.1485399/94‑1 (MQ=60)
             GACCAGATCGCCCATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCA                                                                                <  SRR4302535.3165354/94‑1 (MQ=60)
             GACCAGATCGCCCATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCA                                                                                <  SRR4302535.466018/94‑1 (MQ=60)
             GACCAGATCCCCCATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCA                                                                                <  SRR4302535.701660/94‑1 (MQ=60)
              ACCAGATCGCCCATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCAC                                                                               >  SRR4302535.1823527/1‑94 (MQ=60)
               CCAGATCGCCCATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACC                                                                              <  SRR4302535.1562865/94‑1 (MQ=60)
                CAGATCGCCCATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCT                                                                             <  SRR4302535.4698351/94‑1 (MQ=60)
                CAGATCGCCCATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCT                                                                             >  SRR4302535.3758586/1‑94 (MQ=60)
                  GATCGCCCATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGC                                                                           <  SRR4302535.1389748/94‑1 (MQ=60)
                     CGCCCATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGT                                                                        >  SRR4302535.2673512/1‑94 (MQ=60)
                      GCCCATCAGCGTGTTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTT                                                                       <  SRR4302535.3500634/94‑1 (MQ=60)
                      GCCCATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTT                                                                       <  SRR4302535.231042/94‑1 (MQ=60)
                        CAATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTA                                                                     >  SRR4302535.554832/1‑94 (MQ=60)
                         CATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTA                                                                    >  SRR4302535.2038733/1‑94 (MQ=60)
                         CATCAGCGTGGTCATCACGTCATTGAGGGCGATGATCCCCACTACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTATTCACCTGCGGATTTA                                                                    >  SRR4302535.2384115/1‑94 (MQ=60)
                          ATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAA                                                                   >  SRR4302535.1226940/1‑94 (MQ=60)
                          ATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAA                                                                   >  SRR4302535.2298246/1‑94 (MQ=60)
                          ATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAA                                                                   <  SRR4302535.3033571/94‑1 (MQ=60)
                             AGCGTGGTCATCACGTCATTGATGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAAC                                                                <  SRR4302535.117989/94‑1 (MQ=60)
                              GCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACT                                                               <  SRR4302535.1235644/94‑1 (MQ=60)
                               CGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTT                                                              <  SRR4302535.4026936/94‑1 (MQ=60)
                                GTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTT                                                             <  SRR4302535.363532/94‑1 (MQ=60)
                                GTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTT                                                             <  SRR4302535.977628/94‑1 (MQ=60)
                                GTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTT                                                             <  SRR4302535.109852/94‑1 (MQ=60)
                                 TGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTC                                                            <  SRR4302535.1565662/94‑1 (MQ=60)
                                  GGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCC                                                           >  SRR4302535.3369671/1‑94 (MQ=60)
                                   GTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCA                                                          <  SRR4302535.3533921/94‑1 (MQ=60)
                                     CATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAAC                                                        >  SRR4302535.1038594/1‑94 (MQ=60)
                                      ATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACG                                                       <  SRR4302535.4152540/94‑1 (MQ=60)
                                      ATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACG                                                       >  SRR4302535.3119201/1‑94 (MQ=60)
                                      ATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACG                                                       <  SRR4302535.2834200/94‑1 (MQ=60)
                                        CACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCC                                                     >  SRR4302535.4285801/1‑94 (MQ=60)
                                        CACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCC                                                     >  SRR4302535.4487148/1‑94 (MQ=60)
                                         ACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCT                                                    <  SRR4302535.1620971/94‑1 (MQ=60)
                                         ACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCT                                                    <  SRR4302535.3518567/94‑1 (MQ=60)
                                         ACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCT                                                    <  SRR4302535.3443390/94‑1 (MQ=60)
                                          CGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTC                                                   <  SRR4302535.2746741/94‑1 (MQ=60)
                                           GTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCT                                                  >  SRR4302535.943320/1‑94 (MQ=60)
                                           GTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCT                                                  <  SRR4302535.985189/94‑1 (MQ=60)
                                           GTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCT                                                  <  SRR4302535.433696/94‑1 (MQ=60)
                                           GTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCT                                                  >  SRR4302535.4610258/1‑94 (MQ=60)
                                            TCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTG                                                 >  SRR4302535.1663657/1‑94 (MQ=60)
                                              ATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGTGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAA                                               <  SRR4302535.3328053/94‑1 (MQ=60)
                                              ATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAA                                               >  SRR4302535.2687170/1‑94 (MQ=60)
                                                TGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAG                                             <  SRR4302535.2125305/94‑1 (MQ=60)
                                                  AGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGG                                           >  SRR4302535.2358024/1‑94 (MQ=60)
                                                    GGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTT                                         <  SRR4302535.3065462/94‑1 (MQ=60)
                                                     GTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTA                                        >  SRR4302535.2666451/1‑94 (MQ=60)
                                                       GATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAAC                                      <  SRR4302535.178659/94‑1 (MQ=60)
                                                       GATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAAC                                      <  SRR4302535.3082406/94‑1 (MQ=60)
                                                        ATGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACG                                     <  SRR4302535.4009815/94‑1 (MQ=60)
                                                         TGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGT                                    <  SRR4302535.2276040/94‑1 (MQ=60)
                                                         TGATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGT                                    <  SRR4302535.2532632/94‑1 (MQ=60)
                                                          GATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTA                                   >  SRR4302535.3792455/1‑94 (MQ=60)
                                                           ATCCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTAT                                  <  SRR4302535.3157685/94‑1 (MQ=60)
                                                             CCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCC                                <  SRR4302535.706861/94‑1 (MQ=60)
                                                             CCCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCC                                <  SRR4302535.2809470/94‑1 (MQ=60)
                                                              CCCACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGTGATAACGTATCCG                               >  SRR4302535.1903582/1‑94 (MQ=60)
                                                                 ACCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCA                            >  SRR4302535.3293902/1‑94 (MQ=60)
                                                                  CCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCAC                           <  SRR4302535.2762429/94‑1 (MQ=60)
                                                                  CCACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCAC                           <  SRR4302535.1148093/94‑1 (MQ=60)
                                                                   CACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCACA                          <  SRR4302535.1025776/94‑1 (MQ=60)
                                                                   CACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCACA                          >  SRR4302535.4161253/1‑94 (MQ=60)
                                                                    ACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCACAA                         >  SRR4302535.4066457/1‑94 (MQ=60)
                                                                    ACCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCACAA                         >  SRR4302535.1197230/1‑94 (MQ=60)
                                                                     CCAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGTCACAAT                        <  SRR4302535.2802644/94‑1 (MQ=60)
                                                                      CAGCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCACAATC                       >  SRR4302535.4369016/1‑94 (MQ=60)
                                                                        GCGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCACAATCAG                     <  SRR4302535.2327120/94‑1 (MQ=60)
                                                                         CGCGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCACAATCAGC                    >  SRR4302535.4495905/1‑94 (MQ=60)
                                                                           CGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCACAATCAGCGT                  >  SRR4302535.414169/1‑94 (MQ=60)
                                                                           CGTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCACAATCAGCGG                  >  SRR4302535.1399575/1‑94 (MQ=60)
                                                                            GTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACTTATCCGGCACAATCAGCGTG                 <  SRR4302535.1261109/94‑1 (MQ=60)
                                                                            GTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCACAATCAGCGTG                 <  SRR4302535.1052623/94‑1 (MQ=60)
                                                                            GTACTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCACAATCAGCGTG                 <  SRR4302535.4051398/94‑1 (MQ=60)
                                                                               CTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCACAATCAGCGTGTTG              >  SRR4302535.1296955/1‑94 (MQ=60)
                                                                               CTCGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCACAATCAGCGTGTTG              >  SRR4302535.493907/1‑94 (MQ=60)
                                                                                 CGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCACAATCAGCGTGTTGCG            <  SRR4302535.3969286/94‑1 (MQ=60)
                                                                                 CGTTCATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCACAATCAGCGTGTTGCG            <  SRR4302535.2035800/94‑1 (MQ=60)
                                                                                     CATGATCTCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCACAATCAGCGTGTTGCGAATT        <  SRR4302535.3302002/94‑1 (MQ=60)
                                                                                     CATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCACAATCAGCGTGTTGCGAATT        >  SRR4302535.987238/1‑94 (MQ=60)
                                                                                     CATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCACAATCAGCGTGTTGCGAATT        >  SRR4302535.595250/1‑94 (MQ=60)
                                                                                      ATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCACAATCAGCGTGTTGCGAATTT       >  SRR4302535.3865003/1‑94 (MQ=60)
                                                                                      ATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCACAATCAGCGTGTTGCGAATTT       <  SRR4302535.911128/94‑1 (MQ=60)
                                                                                      ATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCACAATCAGCGTGTTGCGAATTT       >  SRR4302535.3818366/1‑94 (MQ=60)
                                                                                      ATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCACAATCAGCGTGTTGCGAATTT       >  SRR4302535.3474100/1‑94 (MQ=60)
                                                                                      ATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCACAATCAGCGTGTTGCGAATTT       >  SRR4302535.4638273/1‑94 (MQ=60)
                                                                                      ATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCACAATCAGCGTGTTGCGAATTT       >  SRR4302535.3628612/1‑94 (MQ=60)
                                                                                      ATGATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCACAATCAGCGTGTTGCGAATTT       >  SRR4302535.2599515/1‑94 (MQ=60)
                                                                                        GATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCACAATCAGCGTGTTGCGAATTTGT     <  SRR4302535.1281243/94‑1 (MQ=60)
                                                                                        GATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATACGGCACAATCAGCGTGTTGCGAATTTGT     >  SRR4302535.2890526/1‑94 (MQ=60)
                                                                                         ATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCACAATCAGCGTGTTGCGAATTTGTA    >  SRR4302535.2819645/1‑94 (MQ=60)
                                                                                         ATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCACAATCAGCGTGTTGCGAATTTGTA    >  SRR4302535.963364/1‑94 (MQ=60)
                                                                                         ATCGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCACAATCAGCGTGTTGCGAATTTGTA    >  SRR4302535.1363710/1‑94 (MQ=60)
                                                                                           CGCCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCACAATCAGCGTGTTGCGAATTTGTACG  <  SRR4302535.2941432/94‑1 (MQ=60)
                                                                                            |                                                                                            
CCAGCCCCTGACCGACCAGATCGCCCATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCACCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCACAATCAGCGTGTTGCGAATTTGTACG  >  NC_000913/4437769‑4437953

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑