Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1873 183.1 9376148 98.2% 9207377 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,465,639 T→C D147D (GAT→GAC)  mgtA → Mg2+ transport ATPase, P‑type 1

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,465,6390TC99.5% 564.2 / NA 182D147D (GAT→GAC) mgtAMg2+ transport ATPase, P‑type 1
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base C (97/83);  total (98/84)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 192 total aligned reads displayed.

TTTATTTGCTGCGGGCGTTATCGCGCTAATGGTCGCTATTTCTACGTTGCTGAACTTTATTCAGGAAGCACGTTCCACTAAAGCGGCAGATGCCCTGAAAGCGATGGTCAGCAATACTGCGACGGTGCTGCGCGTAATTAACGACAAAGGCGAAAATGGCTGGCTGGAGATCCCGATCGA  >  NC_000913/4465549‑4465728
                                                                                          |                                                                                         
tttatttGCTGCGGGCGTTATCGCGCTAATGGTCGCTATTTCTACGTTGCTGAACTTTATTCAGGAAGCACGTTCCACTAAAGCGGCAGACGc                                                                                         >  2:1462109/1‑93 (MQ=255)
tttatttGCTGCGGGCGTTATCGCGCTAATGGTCGCTATTTCTACGTTGCTGAACTTTATTCAGGAAGCACGTTCCACTAAAGCGGCAGACGc                                                                                         >  2:2972335/1‑93 (MQ=255)
 ttatttGCTGCGGGCGTTATCGCGCTAATGGTCGCTATTTCTACGTTGCTGAACTTTATTCAGGAAGCACGTTCCACTAAAGCGGCAGACGcc                                                                                        <  1:309547/93‑1 (MQ=255)
 ttatttGCTGCGGGCGTTATCGCGCTAATGGTCGCTATTTCTACGTTGCTGAACTTTATTCAGGAAGCACGTTCCACTAAAGCGGCAGACGcc                                                                                        <  1:4385451/93‑1 (MQ=255)
 ttatttGCTGCGGGCGTTATCGCGCTAATGGTCGCTATTTCTACGTTGCTGAACTTTATTCAGGAAGCACGTTCCACTAAAGAGGCAGACGcc                                                                                        >  2:1145583/1‑93 (MQ=255)
   atttGCTGCGGGCGTTATCGCGCTAATGGTCGCTATTTCTACGTTGCTGAACTTTATTCAGGAAGCACGTTCCACTAAAGCGGCAGACGCCCt                                                                                      >  1:4103878/1‑93 (MQ=255)
   atttGCTGCGGGCGTTATCGCGCTAATGGTCGCTATTTCTACGTTGCTGAACTTTATTCAGGAAGCACGTTCCACTAAAGCGGCAGACGCCCt                                                                                      >  1:2523777/1‑93 (MQ=255)
    tttGCTGCGGGCGTTATCGCGCTAATGGTCGCTATTTCTACGTTGCTGAACTTTATTCAGGAAGCACGTTCCACTAAAGCGGCAGACGCCCTg                                                                                     >  2:836001/1‑93 (MQ=255)
    tttGCTGCGGGCGTTATCGCGCTAATGGTCGCTATTTCTACGTTGCTGAACTTTATTCAGGAAGCACGTTCCACTAAAGCGGCAGACGCCCTg                                                                                     >  2:480817/1‑93 (MQ=255)
    tttGCTGCGGGCGTTATCGCGCTAATGGTCGCTATTTCTACGTTGCTGAACTTTATTCAGGAAGCACGTTCCACTAAAGCGGCAGACGCCCTg                                                                                     >  1:4433499/1‑93 (MQ=255)
      tgctgcGGGCGTTATCGCGCTAATGGTCGCTATTTCTACGTTGCTGAACTTTATTCAGGAAGCACGTTCCACTAAAGCGGCAGACGCCCTGaa                                                                                   >  1:2952051/1‑93 (MQ=255)
       gctgcGGGCGTTATCGCGCTAATGGTCGCTATTTCTACGTTGCTGAACTTTATTCAGGAAGCACGTTCCACTAAAGCGGCAGACGCCCTGaaa                                                                                  >  2:1036678/1‑93 (MQ=255)
       gctgcGGGCGTTATCGCGCTAATGGTCGCTATTTCTACGTTGCTGAACTTTATTCAGGAAGCACGTTCCACTAAAGCGGCAGACGCCCTGaaa                                                                                  <  1:1278333/93‑1 (MQ=255)
            gggCGTTATCGCGCTAATGGTCGCTATTTCTACGTTGCTGAACTTTATTCAGGAAGCACGTTCCACTAAAGCGGCAGACGCCCTGAAAGCGAt                                                                             <  1:3795279/93‑1 (MQ=255)
            gggCGTTATCGCGCTAATGGTCGCTATTTCTACGTTGCTGAACTTTATTCAGGAAGCACGTTCCACTAAAGCGGCAGACGCCCTGAAAGCGAt                                                                             <  1:3246523/93‑1 (MQ=255)
            gggCGTTATCGCGCTAATGGTCGCTATTTCTACGTTGCTGAACTTTATTCAGGAAGCACGTTCCACTAAAGCGGCAGACGCCCTGAAAGCGAt                                                                             <  1:2450153/93‑1 (MQ=255)
             ggCGTTATCGCGCTAATGGTCGCTATTTCTACGTTGCTGAACTTTATTCAGGAAGCACGTTCCACTAAAGCGGCAGACGCCCTGAAAGCGATg                                                                            >  2:4030721/1‑93 (MQ=255)
              gCGTTATCGCGCTAATGGTCGCTATTTCTACGTTGCTGAACTTTATTCAGGAAGCACGTTCCACTAAAGCGGCAGACGCCCTGAAAGCGATgg                                                                           >  2:3539012/1‑93 (MQ=255)
              gCGTTATCGCGCTAATGGTCGCTATTTCTACGTTGCTGAACTTTATTCAGGAAGCACGTTCCACTAAAGCGGCAGACGCCCTGAAAGCGATgg                                                                           >  2:4177497/1‑93 (MQ=255)
                gTTATCGCGCTAATGGTCGCTATTTCTACGTTGCTGAACTTTATTCAGGAAGCACGTTCCACTAAAGCGGCAGACGCCCTGAAAGCGATGGTc                                                                         <  1:577398/93‑1 (MQ=255)
                gTTATCGCGCTAATGGTCGCTATTTCTACGTTGCTGAACTTTATTCAGGAAGCACGTTCCACTAAAGCGGCAGACGCCCTGAAAGCGATGGTc                                                                         <  2:4344690/93‑1 (MQ=255)
                 ttATCGCGCTAATGGTCGCTATTTCTACGTTGCTGAACTTTATTCAGGAAGCACGTTCCACTAAAGCGGCAGACGCCCTGAAAGCGATGGTCa                                                                        >  2:1201948/1‑93 (MQ=255)
                 ttATCGCGCTAATGGTCGCTATTTCTACGTTGCTGAACTTTATTCAGGAAGCACGTTCCACTAAAGCGGCAGACGCCCTGAAAGCGATGGTCa                                                                        <  1:4289283/93‑1 (MQ=255)
                 ttATCGCGCTAATGGTCGCTATTTCTACGTTGCTGAACTTTATTCAGGAAGCACGTTCCACTAAAGCGGCAGACGCCCTGAAAGCGATGGTCa                                                                        >  1:3563023/1‑93 (MQ=255)
                 ttATCGCGCTAATGGTCGCTATTTCTACGTTGCTGAACTTTATTCAGGAAGCACGTTCCACTAAAGCGGCAGACGCCCTGAAAGCGATGGCCa                                                                        >  1:675707/1‑93 (MQ=255)
                  tATCGCGCTAATGGTCGCTATTTCTACGTTGCTGAACTTTATTCAGGAAGCACGTTCCACTAAAGCGGCAGACGCCCTGAAAGCGATGGTCAg                                                                       >  1:3448865/1‑93 (MQ=255)
                   aTCGCGCTAATGGTCGCTATTTCTACGTTGCTGAACTTTATTCAGGAAGCACGTTCCACTAAAGCGGCAGACGCCCTGAAAGCGATGGTCAGc                                                                      >  2:1833035/1‑93 (MQ=255)
                        gcTAATGGTCGCTATTTCTACGTTGCTGAACTTTATTCAGGAAGCACGTTCCACTAAAGCGGCAGACGCCCTGAAAGCGATGGTCAGCAATAc                                                                 >  1:2634640/1‑93 (MQ=255)
                        gcTAATGGTCGCTATTTCTACGTTGCTGAACTTTATTCAGGAAGCACGTTCCACTAAAGCGGCAGACGCCCTGAAAGCGATGGTCAGCAATAc                                                                 >  1:2398282/1‑93 (MQ=255)
                        gcTAATGGTCGCTATTTCTACGTTGCTGAACTTTATTCAGGAAGCACGTTCCACTAAAGCGGCAGACGCCCTGAAAGCGATGGTCAGCAATAc                                                                 <  1:1684668/93‑1 (MQ=255)
                        gcTAATGGTCGCTATTTCTACGTTGCTGAACTTTATTCAGGAAGCACGTTCCACTAAAGCGGCAGACGCCCTGAAAGCGATGGTCAGCAATAc                                                                 <  1:3457578/93‑1 (MQ=255)
                         cTAATGGTCGCTATTTCTACGTTGCTGAACTTTATTCAGGAAGCACGTTCCACTAAAGCGGCAGACGCCCTGAAAGCGATGGTCAGCAATACt                                                                <  2:2200742/93‑1 (MQ=255)
                         cTAATGGTCGCTATTTCTACGTTGCTGAACTTTATTCAGGAAGCACGTTCCACTAAAGCGGCAGACGCCCTGAAAGCGATGGTCAGCAATACt                                                                <  1:3317578/93‑1 (MQ=255)
                          tAATGGTCGCTATTTCTACGTTGCTGAACTTTATTCAGGAAGCACGTTCCACTAAAGCGGCAGACACCCTGAAAGCGATGGTCAGCAATACTg                                                               >  2:3681647/1‑93 (MQ=255)
                           aaTGGTCGCTATTTCTACGTTGCTGAACTTTATTCAGGAAGCACGTTCCACTAAAGCGGCAGACGCCCTGAAAGCGATGGTCAGCAATACTGc                                                              <  2:3748112/93‑1 (MQ=255)
                           aaTGGTCGCTATTTCTACGTTGCTGAACTTTATTCAGGAAGCACGTTCCACTAAAGCGGCAGACGCCCTGAAAGCGATGGTCAGCAATACTGc                                                              <  2:1545561/93‑1 (MQ=255)
                            aTGGTCGCTATTTCTACGTTGCTGAACTTTATTCAGGAAGCACGTTCCACTAAAGCGGCAGACGCCCTGAAAGCGATGGTCAGCAATACTGCg                                                             <  2:948482/93‑1 (MQ=255)
                              ggTCGCTATTTCTACGTTGCTGAACTTTATTCAGGAAGCACGTTCCACTAAAGCGGCAGACGCCCTGAAAGCGATGGTCAGCAATACTGCGAc                                                           >  2:1121496/1‑93 (MQ=255)
                               gTCGCTATTTCTACGTTGCTGAACTTTATTCAGGAAGCACGTTCCACTAAAGCGGCAGACGCCCTGAAAGCGATGGTCAGCAATACTGCGACg                                                          >  2:1631625/1‑93 (MQ=255)
                                 cGCTATTTCTACGTTGCTGAACTTTATTCAGGAAGCACGTTCCACTAAAGCGGCAGACGCCCTGAAAGCGATGGTCAGCAATACTGCGACGGt                                                        <  2:3191966/93‑1 (MQ=255)
                                 cGCTATTTCTACGTTGCTGAACTTTATTCAGGAAGCACGTTCCACTAAAGCGGCAGACGCCCTGAAAGCGATGGTCAGCAATACGGCGACGGt                                                        <  2:4570122/93‑1 (MQ=255)
                                    tATTTCTACGTTGCTGAACTTTATTCAGGAAGCACGTTCCACTAAAGCGGCAGACGCCCTGAAAGCGATGGTCAGCAATACTGCGACGGtgct                                                     >  1:1059825/1‑93 (MQ=255)
                                    tATTTCTACGTTGCTGAACTTTATTCAGGAAGCACGTTCCACTAAAGCGGCAGACGCCCTGAAAGCGATGGTCAGCAATACTGCGACGGtgct                                                     >  2:2731106/1‑93 (MQ=255)
                                      tttCTACGTTGCTGAACTTTATTCAGGAAGCACGTTCCACTAAAGCGGCAGACGCCCTGAAAGCGATGGTCAGCAATACTGCGACGGtgc                                                      >  2:2126871/1‑90 (MQ=255)
                                      tttCTACGTTGCTGAACTTTATTCAGGAAGCACGTTCCACTAAAGCGGCAGACGCCCTGAAAGCGATGGTCAGCAATACTGCGACGGtgc                                                      <  1:2126871/90‑1 (MQ=255)
                                          tACGTTGCTGAACTTTATTCAGGAAGCACGTTCCACTAAAGCGGCAGACGCCCTGAAAGCGATGGTCAGCAATACTGCGACGGTGCTGCGCGt                                               >  2:4265250/1‑93 (MQ=255)
                                          tACGTTGCTGAACTTTATTCAGGAAGCACGTTCCACTAAAGCGGCAGACGCCCTGAAAGCGATGGTCAGCAATACTGCGACGGTGCTGCGCGt                                               >  2:1410268/1‑93 (MQ=255)
                                          tACGTTGCTGAACTTTATTCAGGAAGCACGTTCCACTAAAGCGGCAGACGCCCTGAAAGCGATGGTCAGCAATACTGCGACGGTGCTGCGCGt                                               <  2:221403/93‑1 (MQ=255)
                                           aCGTTGCTGAACTTTATTCAGGAAGCACGTTCCACTAAAGCGGCAGACGCCCTGAAAGCGATGGTCAGCAATACTGCGACGGTGCTGCGCGTa                                              <  2:2742477/93‑1 (MQ=255)
                                             gTTGCTGAACTTTATTCAGGAAGCACGTTCCACTAAAGCGGCAGACGCCCTGAAAGCGATGGTCAGCAATACTGCGACGGTGCTGCGCGTAAt                                            <  1:1108184/93‑1 (MQ=255)
                                             gTTGCTGAACTTTATTCAGGAAGCACGTTCCACTAAAGCCGCAGACGCCCTGAAAGCGATGGTCAGCAATACTGCGACGGTGCTGCGCGTAAt                                            >  1:1736012/1‑93 (MQ=255)
                                              ttGCTGAACTTTATTCAGGAAGCACGTTCCACTAAAGCGGCAGACGCCCTGAAAGCGATGGTCAGCAATACTGCGACGGTGCTGCGCGTAAtt                                           >  1:761835/1‑93 (MQ=255)
                                               tGCTGAACTTTATTCAGGAAGCACGTTCCACTAAAGCGGCAGACGCCCTGAAAGCGATGGTCAGCAATACTGCGACGGTGCTGCGCGTAATTa                                          <  1:848454/93‑1 (MQ=255)
                                                 cTGAACTTTATTCAGGAAGCACGTTCCACTAAAGCGGCAGACGCCCTGAAAGCGATGGTTAGCAATACTGCGACGGTGCTGCGCGTAATTAAc                                        <  1:2158066/93‑1 (MQ=255)
                                                   gAACTTTATTCAGGAAGCACGTTCCACTAAAGCGGCAGACGCCCTGAAAGCGATGGTCAGCAATACTGCGACGGTGCTGCGCGTAATTAACGa                                      >  1:4348610/1‑93 (MQ=255)
                                                    aaCTTTATTCAGGAAGCACGTTCCACTAAAGCGGCAGACGCCCTGAAAGCGATGGTCAGCAATACTGCGACGGTGCTGCGCGTAATTAACGAc                                     <  1:2356460/93‑1 (MQ=255)
                                                    aaCTTTATTCAGAAAGCACGTTCCACTAAAGCGGCAGACG‑CCTGAAAGCGATGGTCAGCAATACTGCGACGGTGCTGCGCGTAATTAACGACa                                    <  2:2704515/93‑1 (MQ=255)
                                                      cTTTATTCAGGAAGCACGTTCCACTAAAGCGGCAGACGCCCTGAAAGCGATGGTCAGCAATACTGCGACGGTGCTGCGCGTAATTAACGACaa                                   <  1:2034764/93‑1 (MQ=255)
                                                       tttATTCAGGAAGCACGTTCCACTAAAGCGGCAGACGCGCTGAAAGCGATGGTCAGCAGTACTGCGACGGTGCTGCGCGTAATTAACGACaaa                                  >  1:3616677/1‑93 (MQ=255)
                                                       tttATTCAGGAAGCACGTTCCACTAAAGCGGCAGACGCCCTGAAAGCGATGGTCAGCAATACTGCGACGGTGCTGCGCGTAATTAACGACaaa                                  >  1:4546461/1‑93 (MQ=255)
                                                        ttATTCAGGAAGCACGTTCCACTAAAGCGGCAGACGCCCTGAAAGCGATGGTCAGCAATACTGCGACGGTGCTGCGCGTAATTAACGACAAAg                                 <  2:3217361/93‑1 (MQ=255)
                                                         tATTCAGGAAGCACGTTCCACTAAAGCGGCAGACGCCCTGAAAGCGATGGTCAGCAATACTGCGACGGTGCTGCGCGTAATTAACGACAAAgg                                >  1:3011103/1‑93 (MQ=255)
                                                         tATTCAGGAAGCACGTTCCACTAAAGCGGCAGACGCCCTGAAAGCGATGGTCAGCAATACTGCGACGGTGCTGCGCGTAATTAACGACAAAgg                                >  2:2816339/1‑93 (MQ=255)
                                                         tATTCAGGAAGCACGTTCCACTAAAGCGGCAGACGCCCTGAAAGCGATGGTCAGCAATACTGCGACGGTGCTGCGCGTAATTAACGACAAAgg                                <  1:1575999/93‑1 (MQ=255)
                                                         tATTCAGGAAGCACGTTCCACTAAAGCGGCAGACGCCCTGAAAGCGATGGTCAGCAATACTGCGACGGTGCTGCGCGTAATTAACGACAAAgg                                <  2:3629309/93‑1 (MQ=255)
                                                            tCAGGAAGCACGTTCCACTAAAGCGGCAGACGCCCTGAAAGCGATGGTCAGCAATACTGCGACGGTGCTGCGCGTAATTAACGACAAAGGCGa                             >  1:1447234/1‑93 (MQ=255)
                                                              aGGAAGCACGTTCCACTAAAGCGGCAGACGCCCTGAAAGCGATGGTCAGCAATACTGCGACGGTGCTGCGCGTAATTAACGACAAAGGCGaaa                           >  1:3707379/1‑93 (MQ=255)
                                                               ggAATCACGTTCCACTAAAGCGGCAGACGCTCTGAAAGCGATGGTCAGCAATACTGCGACGGTGCTGCGCGTAATTAACGACAAAGGCGaaaa                          <  1:27054/93‑1 (MQ=255)
                                                               ggAAGCACGTTCCACTAAAGCGGCAGACGCCCTGAAAGCGATGGTCAGCAATACTGCGCCGGTGCTGCGCGTAATTAACGACAAAGGCGaaaa                          >  2:1582552/1‑93 (MQ=255)
                                                               ggAAGCACGTTCCACTAAAGCGGCAGACGCCCTGAAAGCGATGGTCAGCAATACTGCGACGGTGCTGCGCGTAATTAACGACAAAGGCGaaaa                          >  1:1313821/1‑93 (MQ=255)
                                                               ggAAGCACGTTCCACTAAAGCGGCAGACGCCCTGAAAGCGATGGTCAGCAATACTGCGACGGTGCTGCGCGTAATTAACGACAAAGGCGaaaa                          >  2:583479/1‑93 (MQ=255)
                                                               ggAAGCACGTTCCACTAAAGCGGCAGACGCCCTGAAAGCGATGGTCAGCAATACTGCGACGGTGCTGCGCGTAATTAACGACAAAGGCGaaaa                          <  1:2785491/93‑1 (MQ=255)
                                                               ggAAGCACGTTCCACTAAAGCGGCAGACGCCCTGAAAGCGATGGTCAGCAATACTGCGACGGTGCTGCGCGTAATTAACGACAAAGGCGaaaa                          <  1:950958/93‑1 (MQ=255)
                                                                gAAGCACGTTCCACTAAAGCGGCAGACGCCCTGAAAGCGATGGTCAGCAATACTGCGACGGTGCTGCGCGTAATTAACGACAAAGGCGAAAAt                         <  1:1637052/93‑1 (MQ=255)
                                                                 aaGCACGTTCCACTAAAGCGGCAGACGCCCTGAAAGCGATGGTCAGCAATACTGCGACGGTGCTGCGCGTAATTAACGACAAAGGCGAAAAtg                        >  2:3357065/1‑93 (MQ=255)
                                                                  aGCACGTTCCACTAAAGCGGCAGACGCCCTGAAAGCGATGGTCAGCAATACTGCGACGGTGCTGCGCGTAATTAACGACAAAGGCGAAAAtgg                       >  2:3293135/1‑93 (MQ=255)
                                                                   gCACGTTCCACTAAAGCGGCAGACGCCCTGAAAGCGATGGTCAGCAATACTGCGACGGTGCTGCGCGTAATTAACGACAAAGGCGAAAAtggc                      >  2:4605778/1‑93 (MQ=255)
                                                                   gCACGTTCCACTAAAGCGGCAGACGCCCTGAAAGCGATGGTCAGCAATACTGCGACGGTGCTGCGCGTAATTAACGACAAAGGCGAAAAtggc                      >  2:3077382/1‑93 (MQ=255)
                                                                    cACGTTCCACTAAAGCGGCAGACGCCCTGAAAGCGATGGTCAGCAATACTGCGACGGTGCTGCGCGTAATTAACGACAAAGGCGAAAAtggct                     <  1:226660/93‑1 (MQ=255)
                                                                    cACGTTCCACTAAAGCGGCAGACGCCCTGAAAGCGATGGTCAGCAATACTGCGACGGTGCTGCGCGTAATTAAAGACAAAGGCGAAAAtggct                     >  2:546173/1‑93 (MQ=255)
                                                                     aCGTTCCACTAAAGCGGCAGACGCCCTGAAAGCGATGGTCAGCAATACTGCGACGGTGCTGCGCGTAATTAACGACAAAGGCGAAAAtggctg                    <  1:3373406/93‑1 (MQ=255)
                                                                     aCGTTCCACTAAAGCGGCAGACGCCCTGAAAGCGATGGTCAGCAATACTGCGACGGTGCTGCGCGTAATTAACGACAAAGGCGAAAAtggctg                    >  1:2848063/1‑93 (MQ=255)
                                                                     aCGTTCCACTAAAGCGGCAGACGCCCTGAAAGCGATGGTCAGCAATACTGCGACGGTGCTGCGCGTAATTAACGACAAAGGCGAAAAtggctg                    <  1:3501390/93‑1 (MQ=255)
                                                                      cGTTCCACTAAAGCGGCAGACGCCCTGAAAGCGATGGTCAGCAATACTGCGACGGTGCTGCGCGTAATTAACGACAAAGGCGAAAAtggctgg                   >  2:2508356/1‑93 (MQ=255)
                                                                      cGTTCCACTAAAGCGGCAGACGCCCTGAAAGCGATGGTCAGCAATACTGCGACGGTGCTGCGCGTAATTAACGACAAAGGCGAAAAtggctgg                   <  1:1954140/93‑1 (MQ=255)
                                                                        ttCCACTAAAGCGGCAGACGCCCTGAAAGCGATGGTCAGCAATACTGCGACGGTGCTGCGCGTAATTAACGACAAAGGCGAAAATggctggct                 <  2:2176950/93‑1 (MQ=255)
                                                                        ttCCACTAAAGCGGCAGACGCCCTGAAAGCGATGGTCAGCAATACTGCGACGGTGCTGCGCGTAATTAACGACAAAGGCGAAAATggctggct                 >  1:2107890/1‑93 (MQ=255)
                                                                        ttCCACTAAAGCGGCAGACGCCCTGAAAGCGATGGTCAGCAATACTGCGACGGTGCTGCGCGTAATTAACGACAAAGGCGAAAATggctggct                 >  1:4445888/1‑93 (MQ=255)
                                                                         tCCACTAAAGCGGCAGACGCCCTGAAAGCGATGGTCAGCAATACTGCGACGGTGCTGCGCGTAATTAACGACAAAGGCGAAAATGgctggctg                >  2:3985783/1‑93 (MQ=255)
                                                                           cACTAAAGCGGCAGACGCCCTGAAAGCGATGGTCAGCAATACTGCGACGGTGCTGCGCGTAATTAACGACAAAGGCGAAAATGGCTGGCTGga              <  2:1918719/93‑1 (MQ=255)
                                                                           cACTAAAGCGGCAGACGCCCTGAAAGCGATGGTCAGCAATACTGCGACGGTGCTGCGCGTAATTAACGACAAAGGCGAAAATGGCTGGCTGga              <  1:4198933/93‑1 (MQ=255)
                                                                            aCTAAAGCGGCAGACGCCCTGAAAGCGATGGTCAGCAATACTGCGACGGTGCTGCGCGTAATTAACGACAAAGGCGAAAATGGCTGGCTGgag             <  1:499461/93‑1 (MQ=255)
                                                                              tAAAGCGGCAGACGCCCTGAAAGCGATGGTCAGCAATACTGCGACGGTGCTGCGCGTAATTAACGACAAAGGCGAAAATGGCTGGCTGGAGAt           >  2:2014595/1‑93 (MQ=255)
                                                                              tAAAGCGGCAGACGCCCTGAAAGCGATGGTCAGCAATACTGCGACGGTGCTGCGCGTAATTAACGACAAAGGCGAAAATGGCTGGCTGGAGAt           <  2:733720/93‑1 (MQ=255)
                                                                               aaaGCGGCAGACGCCCTGAAAGCGATGGTCAGCAATACTGCGACGGTGCTGCGCGTAATTAACGACAAAGGCGAAAATGGCTGGCTGGAGATc          >  1:1232833/1‑93 (MQ=255)
                                                                                aaGCGGCAGACGCCCTGAAAGCGATGGTCAGCAATACTGCGACGGTGCTGCGCGTAATTAACGACAAAGGCGAAAATGGCTGGCTGGAGATcc         >  1:1895809/1‑93 (MQ=255)
                                                                                aaGCGGCAGACGCCCTGAAAGCGATGGTCAGCAATACTGCGACGGTGCTGCGCGTAATTAACGACAAAGGCGAAAATGGCTGGCTGGAGAAcc         >  1:1350499/1‑93 (MQ=255)
                                                                                  gCGGCAGACGCCCTGAAAGCGATGGTCAGCAATACTGCGACGGTGCTGCGCGTAATTAACGACAAAGGCGAAAATGGCTGGCTGGAGATCCCg       >  2:1241573/1‑93 (MQ=255)
                                                                                    ggCAGACGCCCTGAAAGCGATGGTCAGCAATACTGCGACGGTGCTGCGCGTAATTAACGACAAAGGCGAAAATGGCTGGCTGGAGATCCCGAt     <  2:2619340/93‑1 (MQ=255)
                                                                                     gCAGACGCCCTGAAAGCGATGGTCAGCAATACTGCGACGGTGCTGCGCGTAATTAACGACAAAGGCGAAAATGGCTGGCTGGAGATCCCGATc    <  1:3740460/93‑1 (MQ=255)
                                                                                          |                                                                                         
TTTATTTGCTGCGGGCGTTATCGCGCTAATGGTCGCTATTTCTACGTTGCTGAACTTTATTCAGGAAGCACGTTCCACTAAAGCGGCAGATGCCCTGAAAGCGATGGTCAGCAATACTGCGACGGTGCTGCGCGTAATTAACGACAAAGGCGAAAATGGCTGGCTGGAGATCCCGATCGA  >  NC_000913/4465549‑4465728

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AGATTTATTTGCTGCGGGCGTTATCGCGCTAATGGTCGCTATTTCTACGTTGCTGAACTTTATTCAGGAAGCACGTTCCACTAAAGCGGCAGATGCCCTGAAAGCGATGGTCAGCAATACTGCGACGGTGCTGCGCGTAATTAACGACAAAGGCGAAAATGGCTGGCTGGAGATCCCGATCGACC  >  NC_000913/4465546‑4465730
                                                                                             |                                                                                           
AGATTTATTTGCTGCGGGCGTTATCGCGCTAATGGTTGCTATTTCTACGTTGCTGAACTTTATTCAGGAAGCACGTTCCACTAAAGCGGCAGAC                                                                                             <  SRR4302535.1136839/94‑1 (MQ=60)
AGATTTATTTGCTGCGGGCGTTATCGCGCTAATGGTCGCTATTTCTACGTTGCTGAACTTTATTCAGGAAGCACGTTCCACTAAAGCGGCAGAC                                                                                             >  SRR4302535.3204249/1‑94 (MQ=60)
 GATTTATTTGCTGCGGGCGTTATCGCGCTAATGGTCGCTATTTCTACGTTGCTGAACTTTATTCAGGAAGCACGTTCCACTAAAGCGGCAGACG                                                                                            <  SRR4302535.4343861/94‑1 (MQ=60)
    TTATTTGCTGCGGGCGTTATCGCGCTAATGGTCGCTATTTCTACGTTGCTGAACTTTATTCAGGAAGCACGTTCCACTAAAGCGGCAGACGCCC                                                                                         <  SRR4302535.311695/94‑1 (MQ=60)
    TTATTTGCTGCGGGCGTTATCGCGCTAATGGTCGCTATTTCTACGTTGCTGAACTTTATTCAGGAAGCACGTTCCACTAAAGCGGCAGACGCCC                                                                                         <  SRR4302535.4415453/94‑1 (MQ=60)
     TATTTGCTGCGGGCGTTATCGCGCTAATGGTCGCTATTTCTACGTTGCTGAACTTTATTCAGGAAGCACGTTCCACTAAAGCGGCAGACGCCCT                                                                                        >  SRR4302535.2541964/1‑94 (MQ=60)
     TATTTGCTGCGGGCGTTATCGCGCTAATGGTCGCTATTTCTACGTTGCTGAACTTTATTCAGGAAGCACGTTCCACTAAAGCGGCAGACGCCCT                                                                                        >  SRR4302535.4131973/1‑94 (MQ=60)
     TATTTGCTGCGGGCGTTATCGCGCTAATGGTCGCTATTTCTACGTTGCTGAACTTTATTCAGGAAGCACGTTCCACTAAAGCGGCAGACGCCCT                                                                                        <  SRR4302535.601568/94‑1 (MQ=60)
      ATTTGCTGCGGGCGTTATCGCGCTAATGGTCGCTATTTCTACGTTGCTGAACTTTATTCAGGAAGCACGTTCCACTAAAGCGGCAGACGCCCTG                                                                                       >  SRR4302535.4463878/1‑94 (MQ=60)
        TTGCTGCGGGCGTTATCGCGCTAATGGTCGCTATTTCTACGTTGCTGAACTTTATTCAGGAAGCACGTTCCACTAAAGCGGCAGACGCCCTGAA                                                                                     >  SRR4302535.2972788/1‑94 (MQ=60)
          GCTGCGGGCGTTATCGCGCTAATGGTCGCTATTTCTACGTTGCTGAACTTTATTCAGGAAGCACGTTCCACTAAAGCGGCAGACGCCCTGAAAG                                                                                   <  SRR4302535.1287462/94‑1 (MQ=60)
           CTGCGGGCGTTATCGCGCTAATGGTCGCTATTTCTACGTTGCTGAACTTTATTCAGGAAGCACGTTCCACTAAAGCGGCAGACGCCCTGAAAGC                                                                                  <  SRR4302535.2751152/94‑1 (MQ=60)
              CGGGCGTTATCGCGCTAATGGTCGCTATTTCTACGTTGCTGAACTTTATTCAGGAAGCACGTTCCACTAAAGCGGCAGACGCCCTGAAAGCGAT                                                                               <  SRR4302535.1271053/94‑1 (MQ=60)
              CGGGCGTTATCGCGCTAATGGTCGCTATTTCTACGTTGCTGAACTTTATTCAGGAAGCACGTTCCACTAAAGCGGCAGACGCCCTGAAAGCGAT                                                                               <  SRR4302535.3476288/94‑1 (MQ=60)
               GGGCGTTATCGCGCTAATGGTCGCTATTTCTACGTTGCTGAACTTTATTCAGGAAGCACGTTCCACTAAAGCGGCAGACGCCCTGAAAGCGATG                                                                              <  SRR4302535.3269068/94‑1 (MQ=60)
               GGGCGTTATCGCGCTAATGGTCGCTATTTCTACGTTGCTGAACTTTATTCAGGAAGCACGTTCCACTAAAGCGGCAGACGCCCTGAAAGCGATG                                                                              <  SRR4302535.2467808/94‑1 (MQ=60)
               GGGCGTTATCGCGCTAATGGTCGCTATTTCTACGTTGCTGAACTTTATTCAGGAAGCACGTTCCACTAAAGCGGCAGACGCCCTGAAAGCGATG                                                                              <  SRR4302535.3821441/94‑1 (MQ=60)
                GGCGTTATCGCGCTAATGGTCGCTATTTCTACGTTGCTGAACTTTATTCAGGAAGCACGTTCCACTAAAGCGGCAGACGCCCTGAAAGCGATGG                                                                             >  SRR4302535.94662/1‑94 (MQ=60)
                  CGTTATCGCGCTAATGGTCGCTATTTCTACGTTGCTGAACTTTATTCAGGAAGCACGTTCCACTAAAGCGGCAGACGCCCTGAAAGCGATGGTC                                                                           >  SRR4302535.2124150/1‑94 (MQ=60)
                  CGTTATCGCGCTAATGGTCGCTATTTCTACGTTGCTGAACTTTATTCAGGAAGCACGTTCCACTAAAGCGGCAGACGCCCTGAAAGCGATGGTC                                                                           <  SRR4302535.261850/94‑1 (MQ=60)
                   GTTATCGCGCTAATGGTCGCTATTTCTACGTTGCTGAACTTTATTCAGGAAGCACGTTCCACTAAAGCGGCAGACGCCCTGAAAGCGATGGTCA                                                                          >  SRR4302535.3587726/1‑94 (MQ=60)
                   GTTATCGCGCTAATGGTCGCTATTTCTACGTTGCTGAACTTTATTCAGGAAGCACGTTCCACTAAAGCGGCAGACGCCCTGAAAGCGATGGTCA                                                                          <  SRR4302535.581453/94‑1 (MQ=60)
                   GTTATCGCGCTAATGGTCGCTATTTCTACGTTGCTGAACTTTATTCAGGAAGCACGTTCCACTAAAGCGGCAGACGCCCTGAAAGCGATGGCCA                                                                          >  SRR4302535.680429/1‑94 (MQ=60)
                    TTATCGCGCTAATGGTCGCTATTTCTACGTTGCTGAACTTTATTCAGGAAGCACGTTCCACTAAAGCGGCAGACGCCCTGAAAGCGATGGTCAG                                                                         >  SRR4302535.3472843/1‑94 (MQ=60)
                    TTATCGCGCTAATGGTCGCTATTTCTACGTTGCTGAACTTTATTCAGGAAGCACGTTCCACTAAAGCGGCAGACGCCCTGAAAGCGATGGTCAG                                                                         <  SRR4302535.4318620/94‑1 (MQ=60)
                    TTATCGCGCTAATGGTCGCTATTTCTACGTTGCTGAACTTTATTCAGGAAGCACGTTCCACT‑AAGCGGGCGACGCCCTGAAAGCGATGGTTAGC                                                                        >  SRR4302535.572372/1‑94 (MQ=60)
                          CGCTAATGGTCGCTATTTCTACGTTGCTGAACTTTATTCAGGAAGCACGTTCCACTAAAGCGGCAGACGCCCTGAAAGCGATGGTCAGCAATAC                                                                   >  SRR4302535.2653522/1‑94 (MQ=60)
                          CGCTAATGGTCGCTATTTCTACGTTGCTGAACTTTATTCAGGAAGCACGTTCCACTAAAGCGGCAGACGCCCTGAAAGCGATGGTCAGCAATAC                                                                   >  SRR4302535.2415526/1‑94 (MQ=60)
                           GCTAATGGTCGCTATTTCTACGTTGCTGAACTTTATTCAGGAAGCACGTTCCACTAAAGCGGCAGACGCCCTGAAAGCGATGGTCAGCAATACT                                                                  <  SRR4302535.1696867/94‑1 (MQ=60)
                           GCTAATGGTCGCTATTTCTACGTTGCTGAACTTTATTCAGGAAGCACGTTCCACTAAAGCGGCAGACGCCCTGAAAGCGATGGTCAGCAATACT                                                                  <  SRR4302535.3481616/94‑1 (MQ=60)
                            CTAATGGTCGCTATTTCTACGTTGCTGAACTTTATTCAGGAAGCACGTTCCACTAAAGCGGCAGACGCCCTGAAAGCGATGGTCAGCAATACTG                                                                 <  SRR4302535.3340662/94‑1 (MQ=60)
                             TAATGGTCGCTATTTCTACGTTGCTGAACTTTATTCAGGAAGCACGTTCCACTAAAGCGGCAGACGCCCTGAAAGCGATGGTCAGCAATACTGC                                                                >  SRR4302535.4197092/1‑94 (MQ=60)
                             TAATGGTCGCTATTTCTACGTTGCTGAACTTTATTCAGGAAGCACGTTCCACTAAAGCGGCAGACGCCCTGAAAGCGATGGTCAGCAATACTGC                                                                <  SRR4302535.1389996/94‑1 (MQ=60)
                             TAATGGTCGCTATTTCTACGTTGCTGAACTTTATTCAGGAAGCACGTTCCACTAAAGCGGCAGACGACCTGCAAGCGATGGTCAGCAATACTGC                                                                >  SRR4302535.2372501/1‑94 (MQ=60)
                               ATGGTCGCTATTTCTACGTTGCTGAACTTTATTCAGGAAGCACGTTCCACTAAAGCGGCAGACGCCCTGAAAGCGATGGTCAGCAATACTGCGA                                                              <  SRR4302535.3097840/94‑1 (MQ=60)
                                 GGTCGCTATTTCTACGTTGCTGAACTTTATTCAGGAAGCACGTTCCACTAAAGCGGCAGACGCCCTGAAAGCGATGGTCAGCAATACTGCGACG                                                            >  SRR4302535.186444/1‑94 (MQ=60)
                                      GTATTTCTACGTTGCTGAACTTTATTCAGGAAGCACGTTCCACTAAAGCGGCAGACGCCCTGAAAGCGATGGTCAGCAATACTGCGACGGTGCT                                                       <  SRR4302535.2142205/94‑1 (MQ=60)
                                      CTATTTCTACGTTGCTGAACTTTATTCAGGAAGCACGTTCCACTAAAGCGGCAGACGCCCTGAAAGCGATGGTCAGCAATACTGCGACGGTGCT                                                       <  SRR4302535.3463139/94‑1 (MQ=60)
                                      CTATTTCTACGTTGCTGAACTTTATTCAGGAAGCACGTTCCACTAAAGCGGCAGACGCCCTGAAAGCGATGGTCAGCAATACTGCGACGGTGCT                                                       >  SRR4302535.244931/1‑94 (MQ=60)
                                      CTATTTCTACGTTGCTGAACTTTATTCAGGAAGCACGTTCCACTAAAGCGGCAGACGCCCTGAAAGCGATGGTCAGCAATACTGCGACGGTGCT                                                       >  SRR4302535.1067371/1‑94 (MQ=60)
                                       TATTTCTACGTTGCTGAACTTTATTCAGGAAGCACGTTCCACTAAAGCGGCAGACGCCCTGAAAGCGATGGTCAGCAATACTGCGACGGTGCTG                                                      >  SRR4302535.4232491/1‑94 (MQ=60)
                                       TATTTCTACGTTGCTGAACTTTATTCAGGAAGCACGTTCCACTAAAGCGGCAGACGCCCTGAAAGCGATGGTCAGCAATACTGCGACGGTGCTG                                                      >  SRR4302535.3283290/1‑94 (MQ=60)
                                         TTTCTACGTTGCTGAACTTTATTCAGGAAGCACGTTCCACTAAAGCGGCAGACGCCCTGAAAGCGATGGTCAGCAATACTGCGACGGTGCTGCG                                                    <  SRR4302535.1712741/94‑1 (MQ=60)
                                         TTTCTACGTTGCTGAACTTTATTCAGGAAGCACGTTCCACTAAAGCGGCAGACGCCCTGAAAGCGATGGTCAGCAATACTGCGACGGTGCTGCG                                                    <  SRR4302535.3973861/94‑1 (MQ=60)
                                         TTTCTACGTTGCTGAACTTTATTCAGGAAGCACGTTCCACTAAAGCGGCAGACGCCCTGAAAGCGATGGTCAGCAATACTGCGACGGTGCTGCG                                                    <  SRR4302535.2993257/94‑1 (MQ=60)
                                           TCTACGTTGCTGAACTTTATTCAGGAAGCACGTTCCACTAAAGCGGCAGACGCCCTGAAAGCGATGGTCAGCAATACTGCGACGGTGCTGCGCG                                                  >  SRR4302535.1359123/1‑94 (MQ=60)
                                            CTACGTTGCTGAACTTTATTCAGGAAGCACGTTCCACTAAAGCGGCAGACGCCCTGAAAGCGATGGTCAGCAATACTGCGACGGTGCTGCGCGT                                                 >  SRR4302535.2166975/1‑94 (MQ=60)
                                            CTACGTTGCTGAACTTTATTCAGGAAGCACGTTCCACTAAAGCGGCAGACGCCCTGAAAGCGATGGTCAGCAATACTGCGACGGTGCTGCGCGT                                                 >  SRR4302535.1309108/1‑94 (MQ=60)
                                              ACGTTGCTGAACTTTATTCAGGAAGCACGTTCCACTAAAGCGGCAGACGCCCTGAAAGCGATGGTCAGCAATACTGCGACGGTGCTGCGCGTAA                                               >  SRR4302535.4567699/1‑94 (MQ=60)
                                               CGTTGCTGAACTTTATTCAGGAAGCACGTTCCACTAAAGCGGCAGACGCCCTGAAAGCGATGGTCAGCAATACTGCGACGCTGCTGCGCGTAAT                                              >  SRR4302535.447034/1‑94 (MQ=60)
                                               CGTTGCTGAACTTTATTCAGGAAGCACGTTCCACTAAAGCCGCAGACGCCCTGAAAGCGATGGTCAGCAATACTGCGACGGTGCTGCGCGTAAT                                              >  SRR4302535.1748560/1‑94 (MQ=60)
                                                GTTGCTGAACTTTATTCAGGAAGCACGTTCCACTAAAGCGGCAGACGCCCTGAAAGCGATGGTCAGCAATACTGCGACGGTGCTGCGCGTAATT                                             >  SRR4302535.767205/1‑94 (MQ=60)
                                                GTTGCTGAACTTTATTCAGGAAGCACGTTCCACTAAAGCGGCAGACGCCCTGAAAGCGATGGTCAGCAATACTGCGACGGTGCTGCGCGTAATT                                             <  SRR4302535.1116099/94‑1 (MQ=60)
                                                GTTGCTGAACTTTATTCAGGAAGCACGTTCCACTAAAGCGGCAGACGCCCTGAAAGCGATGGTCAGCAATACTGCGACGGTGCTGCGCGTAATT                                             >  SRR4302535.4365098/1‑94 (MQ=60)
                                                  TGCTGAACTTTATTCAGGAAGCACGTTCCACTAAAGCGGCAGACGCCCTGAAAGCGATGGTCAGCAATACTGCGACGGTGCTGCGCGTAATTAA                                           <  SRR4302535.854422/94‑1 (MQ=60)
                                                    CTGAACTTTATTCAGGAAGCACGTTCCACTAAAGCGGCAGACGCCCTGAAAGCGATGGTTAGCAATACTGCGACGGTGCTGCGCGTAATTAACG                                         <  SRR4302535.2173575/94‑1 (MQ=60)
                                                    CTGAACTTTATTCAGGAAGCACGTTCCACTAAAGCGGCAGACGCCCTGAAAGCGATGGTCAGCAATACTGCGACGGTGCTGCGCGTAATTAACG                                         >  SRR4302535.4632045/1‑94 (MQ=60)
                                                    CTGAACTTTATTCAGGAAGCACGTTCCACTAAAGCGGCAGACGCCCTGAAAGCGATGGTCAGCAATACTGCGACGGTGCTGCGCGTAATTAACG                                         <  SRR4302535.2834938/94‑1 (MQ=60)
                                                     TGAACTTTATTCAGGAAGCACGTTCCACTAAAGCGGCAGACGCCCTGAAAGCGATGGTCAGCAATACTGCGACGGTGCTGCGCGTAATTAACGA                                        >  SRR4302535.4378361/1‑94 (MQ=60)
                                                      GAACTTTATTCAGGAAGCACGTTCCACTAAAGCGGCAGACGCCCTGAAAGCGATGGTCAGCAATACTGCGACGGTGCTGCGCGTAATTAACGAC                                       >  SRR4302535.3567405/1‑94 (MQ=60)
                                                       AACTTTATTCAGGAAGCACGTTCCACTAAAGCGGCAGACGCCCTGAAAGCGATGGTCAGCAATACTGCGACGGTGCTGCGCGTAATTAACGACA                                      <  SRR4302535.2373359/94‑1 (MQ=60)
                                                         CTTTATTCAGGAAGCACGTTCCACTAAAGCGGCAGACGCGCTGAAAGCGATGGTCAGCAGTACTGCGACGGTGCTGCGCGTAATTAACGACAAA                                    >  SRR4302535.3641750/1‑94 (MQ=60)
                                                         CTTTATTCAGGAAGCACGTTCCACTAAAGCGGCAGACGCCCTGAAAGCGATGGTCAGCAATACTGCGACGGTGCTGCGCGTAATTAACGACAAA                                    <  SRR4302535.2049423/94‑1 (MQ=60)
                                                         CTTTATTCAGGAAGCACGTTCCACTAAAGCGGCAGACGCCCTGAAAGCGATGGTCAGCAATACTGCGACGGTGCTGCGCGTAATTAACGACAAA                                    >  SRR4302535.4577548/1‑94 (MQ=60)
                                                         CTTTATTCAGGAAGCACGTTCCACTAAAGCGGCAGACGCCCTGAAAGCGATGGTCAGCAATACTGCGACGGTGCTGCGCGTAATTAACGACAAA                                    >  SRR4302535.3602329/1‑94 (MQ=60)
                                                           TTATTCAGGAAGCACGTTCCACTAAAGCGGCAGACGCCCTGAAAGCGATGGTCAGCAATACTGCGACGGTGCTGCGCGTAATTAACGACAAAGG                                  >  SRR4302535.3032200/1‑94 (MQ=60)
                                                            TATTCAGGAAGCACGTTCCACTAAAGCGGCAGACGCCCTGAAAGCGATGGTCAGCAATACTGCGACGGTGCTGCGCGTAATTAACGACAAAGGC                                 <  SRR4302535.1587367/94‑1 (MQ=60)
                                                             ATTCAGGAAGCACGTTCCACTAAAGCGGCAGACGCCCTGAAAGCGATGGTCAGCAATACTGCGACGGTGCTGCGCGTAATTAACGACAAAGGCG                                >  SRR4302535.325367/1‑94 (MQ=60)
                                                             ATTCAGGAAGCACGTTCCACTAAAGCGGCAGACGCCCTGAAAGCGATGGTCAGCAATACTGCGACGGTGCTGCGCGTAATTAACGACAAAGGCG                                <  SRR4302535.115019/94‑1 (MQ=60)
                                                              TTCAGGAAGCACGTTCCACTAAAGCGGCAGACGCCCTGAAAGCGATGGTCAGCAATACTGCGACGGTGCTGCGCGTAATTAACGACAAAGGCGA                               >  SRR4302535.1457583/1‑94 (MQ=60)
                                                               TCAGGAAGCACGTTCCACTAAAGCGGCAGACGCCCTGAAAGCGATGGTCAGCAATACTGCGACGGTGCTGCGCGTAATAAACGACAAAGGCGAA                              <  SRR4302535.1914019/94‑1 (MQ=60)
                                                                CAGGAAGCACGTTCCACTAAAGCGGCAGACGCCCTGAAAGCGATGGTCAGCAATACTGCGACGGTGCTGCGCGTAATTAACGACAAAGGCGAAA                             >  SRR4302535.3733047/1‑94 (MQ=60)
                                                                 AGGAAGCACGTTCCACTAAAGCGGCAGACGCCCTGAAAGCGATGGTCAGCAATACTGCGACGGTGCTGCGCGTAATTAACGACAAAGGCGAAAA                            >  SRR4302535.1323210/1‑94 (MQ=60)
                                                                  GGAATCACGTTCCACTAAAGCGGCAGACGCTCTGAAAGCGATGGTCAGCAATACTGCGACGGTGCTGCGCGTAATTAACGACAAAGGCGAAAAT                           <  SRR4302535.27267/94‑1 (MQ=60)
                                                                  GGAAGCACGTTCCACTAAAGCGGCAGACGCCCTGAAAGCGATGGTCAGCAATACTGCGACGGTGCTGCGCGTAATTAACGACAAAGGCGAAAAT                           <  SRR4302535.957689/94‑1 (MQ=60)
                                                                  GGAAGCACGTTCCACTAAAGCGGCAGACGCCCTGAAAGCGATGGTCAGCAATACTGCGACGGTGCTGCGCGTAATTAACGACAAAGGCGAAAAT                           <  SRR4302535.378342/94‑1 (MQ=60)
                                                                  GGAAGCACGTTCCACTAAAGCGGCAGACGCCCTGAAAGCGATGGTCAGCAATACTGCGACGGTGCTGCGCGTAATTAACGACAAAGGCGAAAAT                           <  SRR4302535.2805311/94‑1 (MQ=60)
                                                                   GAAGCACGTTCCACTAAAGCGGCAGACGCCCTGAAAGCGATGGTCAGCAATACTGCGACGGTGCTGCGCGTAATTAACGACAAAGGCGAAAATG                          >  SRR4302535.2682927/1‑94 (MQ=60)
                                                                   GAAGCACGTTCCACTAAAGCGGCAGACGCCCTGAAAGCGATGGTCAGCAATACTGCGACGGTGCTGCGCGTAATTAACGACAAAGGCGAAAATG                          <  SRR4302535.1648879/94‑1 (MQ=60)
                                                                    AAGCACGTTCCACTAAAGCGGCAGACGCCCTGAAAGCGATGGTCAGCAATACTGCGACGGTGCTGCGCGTAATTAACGACAAAGGCGAAAATGG                         >  SRR4302535.441809/1‑94 (MQ=60)
                                                                      GCACGTTCCACTAAAGCGGCAGACGCCCTGAAAGCGATGGTCAGCAATACTGCGACGGTGCTGCGCGTAATTAACGACAAAGGCGAAAATGGCT                       >  SRR4302535.2257124/1‑94 (MQ=60)
                                                                       CACGTTCCACTAAAGCGGCAGACGCCCTGAAAGCGATGGTCAGCAATACTGCGACGGTGCTGCGCGTAATTAACGACAAAGGCGAAAATGGCTG                      <  SRR4302535.228220/94‑1 (MQ=60)
                                                                       CACGTTCCACTAAAGCGGCAGACGCCCTGAAAGCGATGGTCAGCAATACTGCGACGGTGCTGCGCGTAATTAACGACAAAGGCGAAAATGGCTG                      >  SRR4302535.2868248/1‑94 (MQ=60)
                                                                        ACGTTCCACTAAAGCGGCAGACGCCCTGAAAGCGATGGTCAGCAATACTGCGACGGTGCTGCGCGTAATTAACGACAAAGGCGAAAATGGCTGG                     <  SRR4302535.3667859/94‑1 (MQ=60)
                                                                        ACGTTCCACTAAAGCGGCAGACGCCCTGAAAGCGATGGTCAGCAATACTGCGACGGTGCTGCGCGTAATTAACGACAAAGGCGAAAATGGCTGG                     <  SRR4302535.3396869/94‑1 (MQ=60)
                                                                        ACGTTCCACTAAAGCGGCAGACGCCCTGAAAGCGATGGTCAGCAATACTGCGACGGTGCTGCGCGTAATTAACGACAAAGGCGAAAATGGCTGG                     <  SRR4302535.3525731/94‑1 (MQ=60)
                                                                         CGTTCCACTAAAGCGGCAGACGCCCTGAAAGCGATGGTCAGCAATACTGCGACGGTGCTGCGCGTAATTAACGACAAAGGCGAAAATGGCTGGC                    <  SRR4302535.1968204/94‑1 (MQ=60)
                                                                          GTTCCACTAAAGCGGCAGACGCCCTGAAAGCGATGGTCAGCAATACTGCGACGGTGCTGCGCGTAATTAACGACAAAGGCGAAAATGGCTGGCT                   >  SRR4302535.2477579/1‑94 (MQ=60)
                                                                          GTTCCACTAAAGCGGCAGACGCCCTGAAAGCGATGGTCAGCAATACTGCGACGGTGCTGCGCGTAATTAACGACAAAGGCGAAAATGGCTGGCT                   >  SRR4302535.770509/1‑94 (MQ=60)
                                                                          GTTCCACTAAAGCGGCAGACGCCCTGAAAGCGATGGTCAGCAATACTGCGACGGTGCTGCGCGTAATTAACGACAAAGGCGAAAATGGCTGGCT                   <  SRR4302535.3360759/94‑1 (MQ=60)
                                                                          GTTCCACTAAAGCGGCAGACGCCCTGAAAGCGATGGTCAGCAATACTGCGACGGTGCTGCGCGTAATTAACGACAAAGGCGAAAATGGCTGGCT                   >  SRR4302535.4476379/1‑94 (MQ=60)
                                                                          GTTCCACTAAAGCGGCAGACGCCCTGAAAGCGATGGTCAGCAATACTGCGACGGTGCTGCGCGTAATTAACGACAAAGGCGAAAATGGCTGGCT                   >  SRR4302535.2123093/1‑94 (MQ=60)
                                                                           TTCCACTAAAGCGGCAGACGCCCTGAAAGCGATGGTCAGCAATACTGCGACGGTGCTGCGCGTAATTAACGACAAAGGCGAAAATGGCTGGCTG                  >  SRR4302535.1627614/1‑94 (MQ=60)
                                                                           TTCCACTAAAGCGGCAGACGCCCTGAAAGCGATGGTCAGCAATACTGCGACGGTGCTGCGCGTAATTAACGACAAAGGCGAAAATGGCTGGCTG                  >  SRR4302535.4386804/1‑94 (MQ=60)
                                                                            TCCACTAAAGCGGCAGACGCCCTGAAAGCGATGGTCAGCAATACTGCGACGGTGCTGCGCGTAATTAACGACAAAGGCGAAAATGGCTGGCTGG                 >  SRR4302535.3488313/1‑94 (MQ=60)
                                                                            TCCACTAAAGCGGCAGACGCCCTGAAAGCGATCGTCAGCAATACTGCGACGGTGCTGCGCGTAATTAACGACAAAGGCGAAAATGGCTGGCTGG                 <  SRR4302535.1577254/94‑1 (MQ=60)
                                                                             CCACTAAAGCGGCAGACGCCCTGAAAGCGATGGTCAGCAATACTGCGACGGTGCTGCGCGTAATTAACGACAAAGGCGAAAATGGCTGGCTGGA                >  SRR4302535.1649508/1‑94 (MQ=60)
                                                                              CACTAAAGCGGCAGACGCCCTGAAAGCGATGGTCAGCAATACTGCGACGGTGCTGCGCGTAATTAACGACAAAGGCGAAAATGGCTGGCTGGAG               >  SRR4302535.210722/1‑94 (MQ=60)
                                                                              CACTAAAGCGGCAGACGCCCTGAAAGCGATGGTCAGCAATACTGCGACGGTGCTGCGCGTAATTAACGACAAAGGCGAAAATGGCTGGCTGGAG               <  SRR4302535.4227668/94‑1 (MQ=60)
                                                                               ACTAAAGCGGCAGACGCCCTGAAAGCGATGGTCAGCAATACTGCGACGGTGCTGCGCGTAATTAACGACAAAGGCGAAAATGGCTGGCTGGAGA              <  SRR4302535.502963/94‑1 (MQ=60)
                                                                                 TAAAGCGGCAGACGCCCTGAAAGCGATGGTCAGCAATACTGCGACGGTGCTGCGCGTAATTAACGACAAAGGCGAAAATGGCTGGCTGGAGATC            >  SRR4302535.1241650/1‑94 (MQ=60)
                                                                                  AAAGCGGCAGACGCCCTGAAAGCGATGGTCAGCAATACTGCGACGGTGCTGCGCGTAATTAACGACAAAGGCGAAAATGGCTGGCTGGAGATCC           >  SRR4302535.1909449/1‑94 (MQ=60)
                                                                                  AAAGCGGCAGACGCCCTGAAAGCGATGGTCAGCAATACTGCGACGGTGCTGCGCGTAATTAACGACAAAGGCGAAAATGGCTGGCTGGAGAACC           >  SRR4302535.1360144/1‑94 (MQ=60)
                                                                                        GCAGACGCCCTGAAAGCGATGGTCAGCAATACTGCGACGGTGCTGCGCGTAATTAACGACAAAGGCGAAAATGGCTGGCTGGAGATCCCGATCG     <  SRR4302535.2405145/94‑1 (MQ=60)
                                                                                        GCAGACGCCCTGAAAGCGATGGTCAGCAATACTGCGACGGTGCTGCGCGTAATTAACGACAAAGGCGAAAATGGCTGGCTGGAGATCCCGATCG     <  SRR4302535.3766348/94‑1 (MQ=60)
                                                                                          AGACGCCCTGAAAGCGATGGTCAGCAATACTGCGACGGTGCTGCGCGTAATTAACGACAAAGGCGAAAATGGCTGGCTGGAGATCCCGATCGAC   >  SRR4302535.1003955/1‑94 (MQ=60)
                                                                                           GACGCCCTGAAAGCGATGGTCAGCAATACTGCGACGGTGCTGCGCGTAATTAACGACAAAGGCGAAAATGGCTGGCTGGAGATCCCGATCGACC  <  SRR4302535.4683270/94‑1 (MQ=60)
                                                                                           GACGCCCTGAAAGCGATGGTCAGCAATACTGCGACGGTGCTGCGCGTAATTAACGACAAAGGCGAAAATGGCTGGCTGGAGATCCCGATCGACC  <  SRR4302535.4403747/94‑1 (MQ=60)
                                                                                           GACGCCCTGAAAGCGATGGTCAGCAATACTGCGACGGTGCTGCGCGTAATTAACGACAAAGGCGAAAATGGCTGGCTGGAGATCCCGATCGACC  >  SRR4302535.3441713/1‑94 (MQ=60)
                                                                                           GACGCCCTGAAAGCGATGGTCAGCAATACTGCGACGGTGCTGCGCGTAATTAACGACAAAGGCGAAAATGGCTGGCTGGAGATCCCGATCGACC  <  SRR4302535.1381490/94‑1 (MQ=60)
                                                                                             |                                                                                           
AGATTTATTTGCTGCGGGCGTTATCGCGCTAATGGTCGCTATTTCTACGTTGCTGAACTTTATTCAGGAAGCACGTTCCACTAAAGCGGCAGATGCCCTGAAAGCGATGGTCAGCAATACTGCGACGGTGCTGCGCGTAATTAACGACAAAGGCGAAAATGGCTGGCTGGAGATCCCGATCGACC  >  NC_000913/4465546‑4465730

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑