Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1873 183.1 9376148 98.2% 9207377 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,621,644 A→G W15R (TGG→CGG)  lplA ← lipoate‑protein ligase A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,621,6440AG99.5% 628.9 / ‑5.5 202W15R (TGG→CGG) lplAlipoate‑protein ligase A
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base G (87/114);  total (87/115)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01

Only 100 of 207 total aligned reads displayed.

CTACCGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTT  >  NC_000913/4621554‑4621733
                                                                                          |                                                                                         
ctacCGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACcgcg                                                                                         <  2:2220145/93‑1 (MQ=255)
 tacCGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCgg                                                                                        >  2:3514508/1‑93 (MQ=255)
  acCGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCggg                                                                                       >  2:2913289/1‑93 (MQ=255)
       gtCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTa                                                                                  >  2:2312860/1‑93 (MQ=255)
         cGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAag                                                                                >  1:1389927/1‑93 (MQ=255)
             aTTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAagagca                                                                            >  2:2444164/1‑91 (MQ=255)
             aTTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTc                                                                            >  1:1034715/1‑93 (MQ=255)
             aTTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTc                                                                            <  2:2696915/93‑1 (MQ=255)
              ttGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCa                                                                           <  2:1180323/93‑1 (MQ=255)
              ttGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCa                                                                           >  2:4550216/1‑93 (MQ=255)
              ttGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCa                                                                           >  1:252839/1‑93 (MQ=255)
              ttGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGCCa                                                                           >  2:3681154/1‑93 (MQ=255)
                 cgcCAGAGAAACAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCagag                                                                        <  1:1371714/93‑1 (MQ=255)
                  gcCAGAGAAACAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAgaga                                                                       <  1:4341539/93‑1 (MQ=255)
                  gcCAGAGAAACAGAACGCGCTGCGTGGCGGGCATTTGGAGAAAAATACCCTCTTCAACCGCCAGGTTAACCCGCGGGTCGTAAGAGTCAgaga                                                                       <  2:4084632/93‑1 (MQ=255)
                   ccAGAGAAACAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGAt                                                                      >  2:530345/1‑93 (MQ=255)
                   ccAGAGAAACAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGAt                                                                      >  1:2777614/1‑93 (MQ=255)
                    cAGAGAAACAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATg                                                                     <  1:1830103/93‑1 (MQ=255)
                    cAGAGAAACAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATg                                                                     >  1:2665130/1‑93 (MQ=255)
                     agagAAACAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGa                                                                    >  1:1300055/1‑93 (MQ=255)
                     agagAAACAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGa                                                                    >  2:234620/1‑93 (MQ=255)
                     agagAAACAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGa                                                                    <  2:4013513/93‑1 (MQ=255)
                     agagAAACAGAACGCGCGGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGa                                                                    <  1:3489401/93‑1 (MQ=255)
                      gagaAACAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAg                                                                   <  1:2396382/93‑1 (MQ=255)
                        gaAACAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCa                                                                 >  1:3888383/1‑93 (MQ=255)
                        gaAACAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCa                                                                 <  1:4164839/93‑1 (MQ=255)
                         aaaCAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAg                                                                >  2:219879/1‑93 (MQ=255)
                         aaaCAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAg                                                                <  2:3371303/93‑1 (MQ=255)
                          aaCAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAgg                                                               <  2:3287850/93‑1 (MQ=255)
                           aCAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGc                                                              <  1:4111327/93‑1 (MQ=255)
                            cAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCg                                                             <  1:120610/93‑1 (MQ=255)
                             aGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGt                                                            <  1:3096098/93‑1 (MQ=255)
                              gAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTa                                                           >  2:1947542/1‑93 (MQ=255)
                               aaCGCGCTGCGTGGCGGGCATTTGGCTAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTaa                                                          >  1:4003522/1‑93 (MQ=255)
                               aaCGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTaa                                                          >  1:3155136/1‑93 (MQ=255)
                                aCGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAACCCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAAt                                                         <  2:2519600/93‑1 (MQ=255)
                                aCGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAAt                                                         >  2:4582418/1‑93 (MQ=255)
                                 cgcgCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAAtg                                                        <  1:3032292/93‑1 (MQ=255)
                                  gcgcTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAAtgt                                                       >  2:2399486/1‑93 (MQ=255)
                                  gcgcTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAAtgt                                                       <  2:1701005/93‑1 (MQ=255)
                                  gcgcTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAAtgt                                                       <  1:2880461/93‑1 (MQ=255)
                                  gcgcTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAAtgt                                                       >  2:3993772/1‑93 (MQ=255)
                                   cgcTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAAtgtg                                                      >  2:267902/1‑93 (MQ=255)
                                   cgcTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAAtgtg                                                      <  2:360873/93‑1 (MQ=255)
                                   cgcTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAAtgtg                                                      <  1:1527380/93‑1 (MQ=255)
                                    gcTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTgg                                                     <  1:3719575/93‑1 (MQ=255)
                                     cTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGa                                                    <  2:669636/93‑1 (MQ=255)
                                     cTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGa                                                    >  1:3679720/1‑93 (MQ=255)
                                     cTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGa                                                    >  2:3413130/1‑93 (MQ=255)
                                      tGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGAc                                                   <  2:4621500/93‑1 (MQ=255)
                                         gTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGGCGTAAGAGTCAGAGTTGAGCGGGCGTAATGTGGACATa                                                >  1:1141473/1‑93 (MQ=255)
                                          tGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATaa                                               <  1:541078/93‑1 (MQ=255)
                                             cGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGa                                            <  2:4182705/93‑1 (MQ=255)
                                              gggCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGAt                                           >  1:57143/1‑93 (MQ=255)
                                               ggCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGAtt                                          >  2:3266358/1‑93 (MQ=255)
                                                gCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGAttt                                         <  2:2588259/93‑1 (MQ=255)
                                                 cATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTc                                        >  1:848499/1‑93 (MQ=255)
                                                 cATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTc                                        <  1:742961/93‑1 (MQ=255)
                                                  aTTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTcc                                       <  2:1250364/93‑1 (MQ=255)
                                                  aTTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTcc                                       <  1:2029432/93‑1 (MQ=255)
                                                   tttGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCt                                      >  1:3930467/1‑93 (MQ=255)
                                                    ttGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCtt                                     >  1:4573318/1‑93 (MQ=255)
                                                    ttGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCtt                                     <  1:99496/93‑1 (MQ=255)
                                                      ggCGAAAACTACACTCTTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTc                                   <  1:1471/93‑1 (MQ=255)
                                                      ggCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTc                                   <  2:567542/93‑1 (MQ=255)
                                                      ggCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTc                                   <  1:1346448/93‑1 (MQ=255)
                                                       gCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCt                                  <  1:917586/93‑1 (MQ=255)
                                                        cGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCtt                                 >  1:2157146/1‑93 (MQ=255)
                                                        cGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCtt                                 >  2:4476858/1‑93 (MQ=255)
                                                        cGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCtt                                 <  1:221351/93‑1 (MQ=255)
                                                        cGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCtt                                 <  1:1259177/93‑1 (MQ=255)
                                                           aaaaTACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGCGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTgta                              >  1:1411012/1‑93 (MQ=255)
                                                              aTACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTgtagta                           >  2:1497484/1‑93 (MQ=255)
                                                               tACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTaa                          >  1:2446641/1‑93 (MQ=255)
                                                               tACACTCTTCCACCGCCAGGCTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTaa                          >  2:2839988/1‑93 (MQ=255)
                                                                acacTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAAt                         <  2:1398004/93‑1 (MQ=255)
                                                                 cacTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATg                        <  2:404311/93‑1 (MQ=255)
                                                                  acTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATgg                       >  2:4167044/1‑93 (MQ=255)
                                                                   ctctTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATggg                      >  2:787524/1‑93 (MQ=255)
                                                                       tCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCAc                  <  1:1737523/93‑1 (MQ=255)
                                                                       tCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCAc                  >  2:2961199/1‑93 (MQ=255)
                                                                         cACGGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCActc                <  1:490160/93‑1 (MQ=255)
                                                                          aCCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCActct               <  2:3773993/93‑1 (MQ=255)
                                                                             gCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCtttt            >  2:2268281/1‑93 (MQ=255)
                                                                             gCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCtttt            <  2:3391837/93‑1 (MQ=255)
                                                                             gCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCtttt            <  2:1114508/93‑1 (MQ=255)
                                                                               cAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCtttttt          >  1:3599260/1‑93 (MQ=255)
                                                                               cAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCtttttt          >  2:3337015/1‑93 (MQ=255)
                                                                                 ggTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTtct        >  1:4532956/1‑93 (MQ=255)
                                                                                  gTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTtctc       <  1:2415986/93‑1 (MQ=255)
                                                                                  gTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTtctc       <  2:2753274/93‑1 (MQ=255)
                                                                                   ttAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCg      >  1:3841985/1‑93 (MQ=255)
                                                                                     aaaCCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCt    <  1:4295262/93‑1 (MQ=255)
                                                                                     aaaCCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCt    <  2:3887972/93‑1 (MQ=255)
                                                                                      aaCCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCtt   >  1:1628832/1‑93 (MQ=255)
                                                                                      aaCCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCtt   >  1:2613962/1‑93 (MQ=255)
                                                                                      aaCCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCtt   >  1:4386299/1‑93 (MQ=255)
                                                                                      aaCCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCtt   <  2:2024819/93‑1 (MQ=255)
                                                                                       aCCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTTTAGTAATGGGTCACTCTTTTTTCTCGCttt  <  1:1806029/93‑1 (MQ=255)
                                                                                       aCCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCttt  <  1:2486085/93‑1 (MQ=255)
                                                                                          |                                                                                         
CTACCGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTT  >  NC_000913/4621554‑4621733

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TTACTACCGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTTCTTC  >  NC_000913/4621551‑4621737
                                                                                             |                                                                                             
TTACTACCGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATTTGGCGTAAAATACACTCTTCCACCGCCAGGTTAAACCG                                                                                               >  SRR4302535.4718632/1‑94 (MQ=60)
   CTACCGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGG                                                                                            <  SRR4302535.2952450/94‑1 (MQ=60)
           TCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAG                                                                                    >  SRR4302535.1399845/1‑94 (MQ=60)
               CATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTC                                                                                >  SRR4302535.1042064/1‑94 (MQ=60)
                ATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCA                                                                               >  SRR4302535.254582/1‑94 (MQ=60)
                  TGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGA                                                                             <  SRR4302535.1420208/94‑1 (MQ=60)
                   GCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAG                                                                            >  SRR4302535.3615559/1‑94 (MQ=60)
                    CGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGA                                                                           <  SRR4302535.1381514/94‑1 (MQ=60)
                     GCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGAT                                                                          >  SRR4302535.2797387/1‑94 (MQ=60)
                     GCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGAT                                                                          <  SRR4302535.4371232/94‑1 (MQ=60)
                      CCAGAGAAACAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATG                                                                         >  SRR4302535.2684212/1‑94 (MQ=60)
                      CCAGAGAAACAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATG                                                                         >  SRR4302535.1339576/1‑94 (MQ=60)
                       CAGAGAAACAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGA                                                                        >  SRR4302535.1309322/1‑94 (MQ=60)
                       CAGAGAAACAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGA                                                                        <  SRR4302535.1843316/94‑1 (MQ=60)
                        AGAGAAACAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAG                                                                       <  SRR4302535.221202/94‑1 (MQ=60)
                        AGAGAAACAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAG                                                                       <  SRR4302535.4012689/94‑1 (MQ=60)
                        AGAGAAACAGAACGCGCGGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAG                                                                       <  SRR4302535.3513646/94‑1 (MQ=60)
                         GAGAAACAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGC                                                                      <  SRR4302535.2413606/94‑1 (MQ=60)
                          AGAAACAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCA                                                                     >  SRR4302535.3915051/1‑94 (MQ=60)
                           GAAACAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAG                                                                    <  SRR4302535.4193358/94‑1 (MQ=60)
                           GAAACAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAG                                                                    <  SRR4302535.1566248/94‑1 (MQ=60)
                              ACAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCG                                                                 <  SRR4302535.4139469/94‑1 (MQ=60)
                               CAGAACGCGCTGCGTGGCGTGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGT                                                                <  SRR4302535.432776/94‑1 (MQ=60)
                               CAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGT                                                                <  SRR4302535.121455/94‑1 (MQ=60)
                                AGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTA                                                               <  SRR4302535.3117660/94‑1 (MQ=60)
                                AGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTA                                                               >  SRR4302535.1179519/1‑94 (MQ=60)
                                 GAACGCGCTGCGTGGCGGGCATTTGGCTAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAA                                                              >  SRR4302535.4030907/1‑94 (MQ=60)
                                 GAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAA                                                              >  SRR4302535.3177052/1‑94 (MQ=60)
                                  AACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAAT                                                             <  SRR4302535.4573195/94‑1 (MQ=60)
                                  AACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAAT                                                             >  SRR4302535.573983/1‑94 (MQ=60)
                                    CGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGT                                                           <  SRR4302535.3053521/94‑1 (MQ=60)
                                     GCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTG                                                          >  SRR4302535.416887/1‑94 (MQ=60)
                                     GCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTG                                                          <  SRR4302535.2900852/94‑1 (MQ=60)
                                     GCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTG                                                          <  SRR4302535.3151907/94‑1 (MQ=60)
                                      CGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGG                                                         <  SRR4302535.1538383/94‑1 (MQ=60)
                                       GCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGA                                                        >  SRR4302535.3705188/1‑94 (MQ=60)
                                       GCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGA                                                        <  SRR4302535.3745341/94‑1 (MQ=60)
                                           CGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGGCGTAAGAGTCAGAGTTGAGCGGGCGTAATGTGGACATA                                                    >  SRR4302535.1149609/1‑94 (MQ=60)
                                            GTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAA                                                   <  SRR4302535.2748650/94‑1 (MQ=60)
                                            GTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAA                                                   <  SRR4302535.4209968/94‑1 (MQ=60)
                                             TGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAAC                                                  >  SRR4302535.215584/1‑94 (MQ=60)
                                             TGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAAC                                                  <  SRR4302535.544857/94‑1 (MQ=60)
                                             TGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAAC                                                  <  SRR4302535.1818095/94‑1 (MQ=60)
                                                CGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGAT                                               >  SRR4302535.57559/1‑94 (MQ=60)
                                                CGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGAT                                               <  SRR4302535.2603506/94‑1 (MQ=60)
                                                 GGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATT                                              <  SRR4302535.127112/94‑1 (MQ=60)
                                                 GGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATT                                              <  SRR4302535.1522583/94‑1 (MQ=60)
                                                   GCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTC                                            >  SRR4302535.854467/1‑94 (MQ=60)
                                                    CATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCC                                           <  SRR4302535.748177/94‑1 (MQ=60)
                                                     ATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCT                                          >  SRR4302535.3957307/1‑94 (MQ=60)
                                                     ATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCT                                          >  SRR4302535.3816999/1‑94 (MQ=60)
                                                     ATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCT                                          >  SRR4302535.3142686/1‑94 (MQ=60)
                                                     ATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCT                                          <  SRR4302535.2044060/94‑1 (MQ=60)
                                                      TTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTT                                         >  SRR4302535.4604493/1‑94 (MQ=60)
                                                       TTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTT                                        <  SRR4302535.100196/94‑1 (MQ=60)
                                                       TTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTT                                        >  SRR4302535.922200/1‑94 (MQ=60)
                                                         GGCGAAAACTACACTCTTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCT                                      <  SRR4302535.1488/94‑1 (MQ=60)
                                                         GGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCT                                      <  SRR4302535.1356061/94‑1 (MQ=60)
                                                         GGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCT                                      >  SRR4302535.734261/1‑94 (MQ=60)
                                                          GCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTT                                     >  SRR4302535.2172643/1‑94 (MQ=60)
                                                          GCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTT                                     <  SRR4302535.924094/94‑1 (MQ=60)
                                                          GCGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTT                                     <  SRR4302535.4381502/94‑1 (MQ=60)
                                                           CGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTG                                    <  SRR4302535.1044533/94‑1 (MQ=60)
                                                           CGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTG                                    <  SRR4302535.2478831/94‑1 (MQ=60)
                                                           CGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTG                                    <  SRR4302535.1268189/94‑1 (MQ=60)
                                                           CGAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTG                                    <  SRR4302535.222877/94‑1 (MQ=60)
                                                            GAAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGT                                   <  SRR4302535.2679475/94‑1 (MQ=60)
                                                             AAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGCGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTA                                  >  SRR4302535.1421088/1‑94 (MQ=60)
                                                             AAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTA                                  >  SRR4302535.3054644/1‑94 (MQ=60)
                                                             AAAAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTA                                  <  SRR4302535.1469944/94‑1 (MQ=60)
                                                               AAATACACTCTTCCACCGGCAGGGTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGT                                <  SRR4302535.3872492/94‑1 (MQ=60)
                                                               AAATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGT                                <  SRR4302535.2872788/94‑1 (MQ=60)
                                                                AATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTTTAGTA                               >  SRR4302535.183759/1‑94 (MQ=60)
                                                                AATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTA                               <  SRR4302535.4049519/94‑1 (MQ=60)
                                                                 ATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAA                              >  SRR4302535.3940778/1‑94 (MQ=60)
                                                                 ATACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAA                              >  SRR4302535.2464263/1‑94 (MQ=60)
                                                                  TACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAAT                             <  SRR4302535.3729960/94‑1 (MQ=60)
                                                                   ACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATG                            <  SRR4302535.252646/94‑1 (MQ=60)
                                                                   ACACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATG                            >  SRR4302535.1849535/1‑94 (MQ=60)
                                                                    atCTCTTCCACCGCCAGGTTAAGCCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATATCCTTTCTTGTAGTAATGG                           <  SRR4302535.3431053/92‑1 (MQ=60)
                                                                     CCTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGG                          <  SRR4302535.528120/94‑1 (MQ=60)
                                                                     ACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGG                          >  SRR4302535.4438943/1‑94 (MQ=60)
                                                                     ACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGG                          <  SRR4302535.1390089/94‑1 (MQ=60)
                                                                     ACTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGG                          <  SRR4302535.487319/94‑1 (MQ=60)
                                                                      CTCTTCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGT                         >  SRR4302535.2007717/1‑94 (MQ=60)
                                                                       TCTGCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTC                        <  SRR4302535.2456537/94‑1 (MQ=60)
                                                                          TCCACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACT                     <  SRR4302535.1750082/94‑1 (MQ=60)
                                                                            CACGGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCT                   <  SRR4302535.493601/94‑1 (MQ=60)
                                                                             ACCGCCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTT                  <  SRR4302535.2430480/94‑1 (MQ=60)
                                                                                 CCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTT              >  SRR4302535.2241770/1‑94 (MQ=60)
                                                                                 CCAGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTT              >  SRR4302535.3624238/1‑94 (MQ=60)
                                                                                   AGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCT            <  SRR4302535.800733/94‑1 (MQ=60)
                                                                                   AGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCT            >  SRR4302535.4563980/1‑94 (MQ=60)
                                                                                   AGGTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCT            >  SRR4302535.1715310/1‑94 (MQ=60)
                                                                                     GTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCG          <  SRR4302535.2433377/94‑1 (MQ=60)
                                                                                     GTTAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCG          >  SRR4302535.3868453/1‑94 (MQ=60)
                                                                                       TAAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCT        <  SRR4302535.138012/94‑1 (MQ=60)
                                                                                        AAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTT       >  SRR4302535.2632709/1‑94 (MQ=60)
                                                                                        AAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTT       <  SRR4302535.3643132/94‑1 (MQ=60)
                                                                                        AAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTT       >  SRR4302535.1640595/1‑94 (MQ=60)
                                                                                        AAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTT       <  SRR4302535.4324652/94‑1 (MQ=60)
                                                                                        AAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTT       <  SRR4302535.4563912/94‑1 (MQ=60)
                                                                                        AAACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTT       >  SRR4302535.4416307/1‑94 (MQ=60)
                                                                                         AACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTT      >  SRR4302535.1235037/1‑94 (MQ=60)
                                                                                          ACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTTTAGTAATGGGTCACTCTTTTTTCTCGCTTTC     <  SRR4302535.1819077/94‑1 (MQ=60)
                                                                                          ACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTTC     <  SRR4302535.2504010/94‑1 (MQ=60)
                                                                                          ACCGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTTC     <  SRR4302535.940530/94‑1 (MQ=60)
                                                                                            CGCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTTCTT   >  SRR4302535.4417741/1‑94 (MQ=60)
                                                                                             GCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTTCTTC  <  SRR4302535.168691/94‑1 (MQ=60)
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TTACTACCGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTTCTTC  >  NC_000913/4621551‑4621737

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑