Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1548 369.5 20095359 96.9% 19472402 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,865,007 A→G T26A (ACC→GCC)  yeaG → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,865,0070AG98.3% 754.3 / 0.4 239T26A (ACC→GCC) yeaGhypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (1/3);  new base G (119/116);  total (120/119)
Fisher's exact test for biased strand distribution p-value = 3.70e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.68e-01

Only 100 of 244 total aligned reads displayed.

AAGGACGGCATACCATGAATATATTCGATCACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTACCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGTGAGCCTGTCATGGTCGATACAGC  >  NC_000913/1864918‑1865095
                                                                                         |                                                                                        
aaGGACGGCATACCATGAATATATTCGATCACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCa                                                                                       <  1:4707403/93‑1 (MQ=255)
  ggACGGCATACCATGAATATATTCGATCACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACt                                                                                     <  2:5341622/93‑1 (MQ=255)
   gACGGCATCCCATGAGTCTCTTCGATCACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACtt                                                                                    <  1:8934134/93‑1 (MQ=255)
   gACGGCATACCATGAATATATTCGATCACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACtt                                                                                    <  2:7379601/93‑1 (MQ=255)
   gACGGCATACCATGAATATATTCGATCACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTACCACtt                                                                                    <  1:3803734/93‑1 (MQ=255)
    aCGGCATACCATGAATATATTCGATCACTATCGCCAGCGATATGAAGCTGCCACGGACGAAGAGGTCACACTGCAGGAGTTTCTTGCCACTTg                                                                                   >  2:5954949/1‑93 (MQ=255)
      ggCATACCATGAATATATTCGATCACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTc                                                                                 <  1:4111410/93‑1 (MQ=255)
       gCATACCATGAATATATTCGATCACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCg                                                                                >  1:8539915/1‑93 (MQ=255)
           aCCATGAATATATTCGATCACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCaa                                                                            >  2:4502791/1‑93 (MQ=255)
           aCCATGAATATATTCGATCACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCaa                                                                            <  2:5588103/93‑1 (MQ=255)
            ccATGAATATATTCGATCACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAg                                                                           >  1:5566955/1‑93 (MQ=255)
            ccATGAATATATTCGATCACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAg                                                                           >  1:1434659/1‑93 (MQ=255)
              aTGAATATATTCGATCACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGAt                                                                         >  2:6167306/1‑93 (MQ=255)
               tGAATATATTCGATCACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATc                                                                        >  1:10038743/1‑93 (MQ=255)
               tGAATATATTCGATCACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATc                                                                        <  2:4795622/93‑1 (MQ=255)
                gAATATATTCGATCACTATCGCCAGCGATATGAAGCTGCCAAGGACGGAGAGTTCACACTGCAGGAGTGTCTTACCCCTTGTCGGCAAGATCg                                                                       >  2:3458774/1‑93 (MQ=255)
                gAATATATTCGATCACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCg                                                                       >  2:8683675/1‑93 (MQ=255)
                gAATATATTCGATCACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCg                                                                       <  2:8515546/93‑1 (MQ=255)
                gAATATATTCGATCACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCg                                                                       >  1:1353770/1‑93 (MQ=255)
                aaaTATATTCGATCACTAGCGCTAGCTATATGAAGCGGCCAAGGACGAAGATTTCACACTGCAGGAGTTTCTGGCCACTTGTCGGCAAGATCg                                                                       <  2:4918358/92‑1 (MQ=255)
                 aaTATATTCGATCACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGc                                                                      <  2:2162497/93‑1 (MQ=255)
                  atatatTCGATCACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCa                                                                     <  1:75117/93‑1 (MQ=255)
                   tatatTCGATCACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAg                                                                    <  1:5533524/93‑1 (MQ=255)
                   tatatTCGATCACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAg                                                                    >  1:1087753/1‑93 (MQ=255)
                   tatatTCGATCACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAg                                                                    <  2:934556/93‑1 (MQ=255)
                   tatatTCGATCACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGAACGCAg                                                                    <  2:8933921/93‑1 (MQ=255)
                     tatTCGATCACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTg                                                                  >  2:179389/1‑93 (MQ=255)
                      atTCGATCACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGc                                                                 >  1:7816602/1‑93 (MQ=255)
                      atTCGATCACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGc                                                                 <  1:6589890/93‑1 (MQ=255)
                       ttCGATCACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCt                                                                <  1:1260658/93‑1 (MQ=255)
                       ttCGATCACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCt                                                                <  1:281304/93‑1 (MQ=255)
                         cGATCACTATCGCCAGCGATATGAAGCTGCCAAGGATGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTa                                                              <  1:1314486/93‑1 (MQ=255)
                         cGATCACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTa                                                              <  2:8070785/93‑1 (MQ=255)
                         cGATCACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTa                                                              >  2:3130053/1‑93 (MQ=255)
                         cGATCACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTa                                                              <  1:169225/93‑1 (MQ=255)
                          gATCACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTAt                                                             <  1:709920/93‑1 (MQ=255)
                           aTCACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATg                                                            >  1:5478630/1‑93 (MQ=255)
                               cTATCGCCGGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCaa                                                        >  1:8352296/1‑93 (MQ=255)
                               cTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCaa                                                        <  1:4581300/93‑1 (MQ=255)
                                tATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAAc                                                       <  1:2109708/93‑1 (MQ=255)
                                 aacGCCATCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAAcg                                                      <  1:2530379/91‑1 (MQ=255)
                                 aTCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAAcg                                                      <  2:9611311/93‑1 (MQ=255)
                                  tCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAAcgc                                                     >  2:393472/1‑93 (MQ=255)
                                  tCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAAcgc                                                     <  2:8285448/93‑1 (MQ=255)
                                     ccAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGc                                                  <  2:7118431/93‑1 (MQ=255)
                                       aGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTg                                                >  2:580464/1‑93 (MQ=255)
                                        gCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGa                                               >  1:2293332/1‑93 (MQ=255)
                                        gCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGa                                               >  1:6950147/1‑93 (MQ=255)
                                        gCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGa                                               >  1:7980294/1‑93 (MQ=255)
                                         cGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAg                                              <  2:7234040/93‑1 (MQ=255)
                                          gATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGc                                             <  1:2670758/93‑1 (MQ=255)
                                          gATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGc                                             <  1:8907733/93‑1 (MQ=255)
                                           atatGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCg                                            >  1:8785280/1‑93 (MQ=255)
                                            tatGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCTAACGCGGCTGAGTgg                                           >  2:8970220/1‑93 (MQ=255)
                                             atGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGc                                          >  2:4263976/1‑93 (MQ=255)
                                               gAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTa                                        <  1:9612578/93‑1 (MQ=255)
                                                aaGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTAt                                       >  1:8504175/1‑93 (MQ=255)
                                                aaGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTAt                                       >  2:9456318/1‑93 (MQ=255)
                                                   cTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGa                                    <  1:4193639/93‑1 (MQ=255)
                                                    tGCCAAGGACGCAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGAt                                   >  2:5218420/1‑93 (MQ=255)
                                                    tGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGACAACGCGGCTGAGCGGCTATTGAt                                   >  2:3964617/1‑93 (MQ=255)
                                                     gCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATg                                  <  1:3226471/93‑1 (MQ=255)
                                                     gCCAAGGACGAAGAGTTCACACTGCAGGAGGTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGGGGCGATTGGTg                                  >  2:1423435/1‑93 (MQ=255)
                                                        aaGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCt                               >  1:7281732/1‑93 (MQ=255)
                                                         aGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTa                              <  1:9583821/93‑1 (MQ=255)
                                                         aGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTa                              <  2:7585508/93‑1 (MQ=255)
                                                          ggACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTAt                             <  1:5314919/93‑1 (MQ=255)
                                                          ggACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTAt                             <  1:9362793/93‑1 (MQ=255)
                                                           gACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATc                            >  1:7686509/1‑93 (MQ=255)
                                                           gACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATc                            <  2:445896/93‑1 (MQ=255)
                                                           gACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATc                            >  2:5461878/1‑93 (MQ=255)
                                                            aCGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCg                           >  2:7819159/1‑93 (MQ=255)
                                                            aCGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCg                           <  2:9134157/93‑1 (MQ=255)
                                                              gAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGt                         <  2:6742158/93‑1 (MQ=255)
                                                              gAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGt                         >  2:2789175/1‑93 (MQ=255)
                                                              gAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGt                         >  2:1599454/1‑93 (MQ=255)
                                                               aaGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGTg                        >  2:6553601/1‑93 (MQ=255)
                                                                 gagTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGTGAg                      >  2:5894065/1‑93 (MQ=255)
                                                                  agTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGTGAGc                     <  2:1051380/93‑1 (MQ=255)
                                                                   gTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGTGAGcc                    >  1:8375199/1‑93 (MQ=255)
                                                                   gTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGTGAGcc                    <  2:6600858/93‑1 (MQ=255)
                                                                      cacaCTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGTGAGCCTGt                 <  1:461548/93‑1 (MQ=255)
                                                                        cacTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGTGAGCCTGTCa               <  2:1922800/93‑1 (MQ=255)
                                                                          cTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGTGAGCCTGTCATg             <  1:3386525/93‑1 (MQ=255)
                                                                           tGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGTGAGCCTGTCATgg            >  1:3283093/1‑93 (MQ=255)
                                                                           tGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGTGAGCCTGTCATgg            >  2:6880497/1‑93 (MQ=255)
                                                                            gCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGTGAGCCTGTCATGGt           <  1:5483896/93‑1 (MQ=255)
                                                                            gCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGTGAGCCTGTCATGGt           <  1:7764030/93‑1 (MQ=255)
                                                                             cAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGTGAGCCTGTCATGGTc          >  1:386597/1‑93 (MQ=255)
                                                                              aGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGTGAGCCTGTCATGGTCg         >  2:3892345/1‑93 (MQ=255)
                                                                               ggAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGTGAGCCTGTCATGGTCGa        >  1:866818/1‑93 (MQ=255)
                                                                               agAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGTGAGCCTGTCATAGTCGa        <  1:475597/92‑1 (MQ=255)
                                                                                 aGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGTGAGCCTGTCATGGTCGATa      >  2:7940371/1‑93 (MQ=255)
                                                                                 aGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGTGAGCCTGTCATGGTCGATa      <  1:9434940/93‑1 (MQ=255)
                                                                                  gTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGTGAGCCTGTCATGGTCGATAc     <  1:6117650/93‑1 (MQ=255)
                                                                                  gTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGTGAGCCTGTCATGGTCGATAc     <  2:256075/93‑1 (MQ=255)
                                                                                  gTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGTGAGCCTGTCATGGTCGATAc     >  1:2311985/1‑93 (MQ=255)
                                                                                   tttCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGTGAGCCTGTCATGGTCGATACa    >  2:6382072/1‑93 (MQ=255)
                                                                                    ttCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGTGAGCCTGTCATGGTCGATACAg   <  2:2474833/93‑1 (MQ=255)
                                                                                     tCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGTGAGCCTGTCATGGTCGATACAGc  <  1:2974873/93‑1 (MQ=255)
                                                                                         |                                                                                        
AAGGACGGCATACCATGAATATATTCGATCACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTACCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGTGAGCCTGTCATGGTCGATACAGC  >  NC_000913/1864918‑1865095

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CGAAAGGACGGCATACCATGAATATATTCGATCACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTACCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGTGAGCCTGTCATGGTCGATACAGCCCAGG  >  NC_000913/1864915‑1865100
                                                                                            |                                                                                             
CGAAAGGACGGCATACCATGAATATATTCGATCACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGC                                                                                              >  SRR4302540.9905783/1‑94 (MQ=60)
CGAAAGGACGGCATACCATGAATATATTCGATCACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGC                                                                                              >  SRR4302540.2669819/1‑94 (MQ=60)
 GAAAGGACGGCATACCATGAATATATTCGATCACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCC                                                                                             <  SRR4302540.2806750/94‑1 (MQ=60)
   AAGGACGGCATACCATGAATATATTCGATCACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCAC                                                                                           <  SRR4302540.4757712/94‑1 (MQ=60)
   AAGGACGGCATACCATGAATATATTCGATCACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCAC                                                                                           >  SRR4302540.8015803/1‑94 (MQ=60)
    AGGACGGCATACCATGAATATATTCGATCACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACT                                                                                          <  SRR4302540.5082525/94‑1 (MQ=60)
     AGACGGCATACCATGAATATATTCGATCACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTT                                                                                         <  SRR4302540.3333829/94‑1 (MQ=60)
      GACGGCATCCCATGAGTCTCTTCGATCACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTG                                                                                        <  SRR4302540.9038587/94‑1 (MQ=60)
      GACGGCATACCATGAATATATTCGATCACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTG                                                                                        >  SRR4302540.5953121/1‑94 (MQ=60)
      GACGGCATACCATGAATATATTCGATCACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTACCACTTG                                                                                        <  SRR4302540.3845167/94‑1 (MQ=60)
       ACGGCATACCATGAATATATTCGATCACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGT                                                                                       <  SRR4302540.445864/94‑1 (MQ=60)
         GGCATACCATGAATATATTCGATCACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCG                                                                                     >  SRR4302540.8639146/1‑94 (MQ=60)
         GGCATACCATGAATATATTCGATCACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCG                                                                                     <  SRR4302540.4155788/94‑1 (MQ=60)
          GCATACCATGAATATATTCGATCACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGG                                                                                    >  SRR4302540.278756/1‑94 (MQ=60)
           CATACCATGAATATATTCGATCACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGC                                                                                   >  SRR4302540.8714123/1‑94 (MQ=60)
            ATACCATGAATATATTCGATCACTATCTCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCA                                                                                  >  SRR4302540.3417316/1‑94 (MQ=60)
            ATACCATGAATATATTCGATCACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCA                                                                                  >  SRR4302540.6799190/1‑94 (MQ=60)
             TACCATGAATATATTCGATCACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAA                                                                                 <  SRR4302540.4710609/94‑1 (MQ=60)
              ACCATGAATATATTCGATCACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAG                                                                                >  SRR4302540.5628256/1‑94 (MQ=60)
              ACCATGAATATATTCGATCACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAG                                                                                >  SRR4302540.1450213/1‑94 (MQ=60)
               CCATGAATATATTCGATCACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGA                                                                               <  SRR4302540.4159516/94‑1 (MQ=60)
                CATGAATATATTCGATCACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGAT                                                                              >  SRR4302540.1704096/1‑94 (MQ=60)
                CATGAATATATTCGATCACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGAT                                                                              <  SRR4302540.2785220/94‑1 (MQ=60)
                CATGAATATATACGATCACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGAT                                                                              <  SRR4302540.254182/94‑1 (MQ=60)
                CATAAATATATTCGATCACTATCGACAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTACCACTTGTCGGCAAGAT                                                                              <  SRR4302540.7103276/94‑1 (MQ=60)
                 ATGAATATATTCGATCACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATC                                                                             >  SRR4302540.10155473/1‑94 (MQ=60)
                  TGAATATATTCGATCACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCG                                                                            >  SRR4302540.1368019/1‑94 (MQ=60)
                  TGAATATATTCGATCACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGGTTCTTGCCACTTGTCGGCAAGATCG                                                                            >  SRR4302540.1368018/1‑94 (MQ=60)
                   GAATATATTCGATCACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGC                                                                           >  SRR4302540.2850185/1‑94 (MQ=60)
                     ATATATTCGATCACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAG                                                                         <  SRR4302540.7792176/94‑1 (MQ=60)
                     ATATATTCGATCACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAG                                                                         >  SRR4302540.1098911/1‑94 (MQ=60)
                     ATATATTCGATCACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAG                                                                         <  SRR4302540.7792178/94‑1 (MQ=60)
                     ATATATTCGATCACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAG                                                                         <  SRR4302540.75894/94‑1 (MQ=60)
                      TATATTCGATCACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGT                                                                        <  SRR4302540.5594460/94‑1 (MQ=60)
                      TATATTCGATCACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGT                                                                        <  SRR4302540.1967869/94‑1 (MQ=60)
                        TATTCGATCACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGC                                                                      <  SRR4302540.9504127/94‑1 (MQ=60)
                        TATTCGATCACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGC                                                                      >  SRR4302540.7907953/1‑94 (MQ=60)
                         ATTCGATCACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCT                                                                     >  SRR4302540.319366/1‑94 (MQ=60)
                         ATTCGATCACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCT                                                                     <  SRR4302540.6662880/94‑1 (MQ=60)
                          TTCGATCACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTT                                                                    <  SRR4302540.284066/94‑1 (MQ=60)
                          TTCGATCACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTT                                                                    <  SRR4302540.1273589/94‑1 (MQ=60)
                          TTCGATCACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTT                                                                    <  SRR4302540.1273590/94‑1 (MQ=60)
                           TCGATCACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTA                                                                   >  SRR4302540.7629365/1‑94 (MQ=60)
                            CGATCACTATCGCCAGCGATATGAAGCTGCCAAGGATGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTAT                                                                  <  SRR4302540.1328153/94‑1 (MQ=60)
                            CGATCACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTAT                                                                  >  SRR4302540.5340744/1‑94 (MQ=60)
                            CGATCACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTAT                                                                  <  SRR4302540.3992391/94‑1 (MQ=60)
                            CGATCACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTAT                                                                  <  SRR4302540.170919/94‑1 (MQ=60)
                            CGATCACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTAT                                                                  <  SRR4302540.8539530/94‑1 (MQ=60)
                             GATCACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATG                                                                 >  SRR4302540.6314362/1‑94 (MQ=60)
                             GATCACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATG                                                                 >  SRR4302540.1305435/1‑94 (MQ=60)
                             GATCACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATG                                                                 >  SRR4302540.5538940/1‑94 (MQ=60)
                             GATCACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATG                                                                 <  SRR4302540.717546/94‑1 (MQ=60)
                              ATCACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGC                                                                >  SRR4302540.1407205/1‑94 (MQ=60)
                                CACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCA                                                              >  SRR4302540.9579104/1‑94 (MQ=60)
                                CACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCA                                                              <  SRR4302540.3297112/94‑1 (MQ=60)
                                 ACTATCGCCGGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAA                                                             >  SRR4302540.8449232/1‑94 (MQ=60)
                                  CTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAAC                                                            <  SRR4302540.4630685/94‑1 (MQ=60)
                                   TATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACG                                                           <  SRR4302540.2132353/94‑1 (MQ=60)
                                    ATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGC                                                          >  SRR4302540.3431377/1‑94 (MQ=60)
                                    AACGCCATCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGC                                                          <  SRR4302540.2557685/94‑1 (MQ=60)
                                       TCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCGGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGC                                                       <  SRR4302540.9443900/94‑1 (MQ=60)
                                         CAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTG                                                     >  SRR4302540.144137/1‑94 (MQ=60)
                                          AGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGA                                                    >  SRR4302540.8073245/1‑94 (MQ=60)
                                          AGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGA                                                    >  SRR4302540.7027482/1‑94 (MQ=60)
                                          AGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGA                                                    <  SRR4302540.1060184/94‑1 (MQ=60)
                                          AGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGA                                                    >  SRR4302540.2318011/1‑94 (MQ=60)
                                          AGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGA                                                    >  SRR4302540.2318010/1‑94 (MQ=60)
                                           GCGATATGAAGCTGCCAAGGACGAATAGTTCACACTGCAGGAGTTTCTTACCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAG                                                   <  SRR4302540.1804095/94‑1 (MQ=60)
                                            CGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGC                                                  >  SRR4302540.4380404/1‑94 (MQ=60)
                                             GATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCG                                                 <  SRR4302540.9010879/94‑1 (MQ=60)
                                             GATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCG                                                 <  SRR4302540.2699685/94‑1 (MQ=60)
                                             GATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCG                                                 <  SRR4302540.9010880/94‑1 (MQ=60)
                                             GATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCG                                                 >  SRR4302540.8886960/1‑94 (MQ=60)
                                             GATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCG                                                 >  SRR4302540.8739758/1‑94 (MQ=60)
                                             GATATGAAGCTGCCAAGGACGAAGAGTTCACACTGAAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCG                                                 >  SRR4302540.8395957/1‑94 (MQ=60)
                                                ATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCT                                              >  SRR4302540.9593855/1‑94 (MQ=60)
                                                 TGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTA                                             >  SRR4302540.5539212/1‑94 (MQ=60)
                                                  GAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTAT                                            <  SRR4302540.9725915/94‑1 (MQ=60)
                                                  GAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTAT                                            <  SRR4302540.4704271/94‑1 (MQ=60)
                                                  GAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTAT                                            >  SRR4302540.2345169/1‑94 (MQ=60)
                                                  GAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTAT                                            >  SRR4302540.8602998/1‑94 (MQ=60)
                                                   AAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATT                                           <  SRR4302540.603532/94‑1 (MQ=60)
                                                      CTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGAT                                        <  SRR4302540.4239259/94‑1 (MQ=60)
                                                      CTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGAT                                        <  SRR4302540.3189623/94‑1 (MQ=60)
                                                      CTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGAT                                        <  SRR4302540.6929907/94‑1 (MQ=60)
                                                        GCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGG                                      <  SRR4302540.3261548/94‑1 (MQ=60)
                                                          CAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCT                                    >  SRR4302540.7362986/1‑94 (MQ=60)
                                                            AGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTAT                                  <  SRR4302540.9696976/94‑1 (MQ=60)
                                                            AGGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTAT                                  <  SRR4302540.5601736/94‑1 (MQ=60)
                                                             GGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATC                                 <  SRR4302540.5373116/94‑1 (MQ=60)
                                                             GGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATC                                 <  SRR4302540.9474288/94‑1 (MQ=60)
                                                             GGACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATC                                 >  SRR4302540.7773718/1‑94 (MQ=60)
                                                               ACGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGG                               >  SRR4302540.5526151/1‑94 (MQ=60)
                                                                CGAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGT                              >  SRR4302540.8938301/1‑94 (MQ=60)
                                                                 GAAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGTG                             >  SRR4302540.8061688/1‑94 (MQ=60)
                                                                  AAGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGTGA                            <  SRR4302540.754505/94‑1 (MQ=60)
                                                                   AGAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGTGAG                           >  SRR4302540.8794867/1‑94 (MQ=60)
                                                                    GAGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGTGAGC                          >  SRR4302540.9069371/1‑94 (MQ=60)
                                                                     AGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGTGAGCC                         >  SRR4302540.8472384/1‑94 (MQ=60)
                                                                     AGTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGTGAGCC                         >  SRR4302540.8472385/1‑94 (MQ=60)
                                                                      GTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGTGAGCCT                        >  SRR4302540.2257389/1‑94 (MQ=60)
                                                                      GTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGTGAGCCT                        >  SRR4302540.9712346/1‑94 (MQ=60)
                                                                      GTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGTGAGCCT                        >  SRR4302540.5559201/1‑94 (MQ=60)
                                                                      GTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGTGAGCCT                        <  SRR4302540.8455354/94‑1 (MQ=60)
                                                                      GTTCACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGTGAGCCT                        >  SRR4302540.8610112/1‑94 (MQ=60)
                                                                         CACACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGTGAGCCTGTC                     <  SRR4302540.466474/94‑1 (MQ=60)
                                                                         CACACTGCAGGAGTTGCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGTGAGCCTGTC                     <  SRR4302540.466473/94‑1 (MQ=60)
                                                                           CACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGTGAGCCTGTCAT                   <  SRR4302540.1199067/94‑1 (MQ=60)
                                                                           CACTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGTGAGCCTGTCAT                   <  SRR4302540.7428086/94‑1 (MQ=60)
                                                                             CTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGTGAGCCTGTCATGG                 >  SRR4302540.3318978/1‑94 (MQ=60)
                                                                             CTGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGTGAGCCTGTCATGG                 <  SRR4302540.3423479/94‑1 (MQ=60)
                                                                              TGCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGTGAGCCTGTCATGGT                >  SRR4302540.2663582/1‑94 (MQ=60)
                                                                               GCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGTGAGCCTGTCATGGTC               >  SRR4302540.390597/1‑94 (MQ=60)
                                                                               GCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGTGAGCCTGTCATGGTC               <  SRR4302540.7854783/94‑1 (MQ=60)
                                                                               GCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGTGAGCCTGTCATGGTC               <  SRR4302540.7854784/94‑1 (MQ=60)
                                                                               GCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGTGAGCCTGTCATGGTC               <  SRR4302540.5544266/94‑1 (MQ=60)
                                                                               GCAGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGTGAGCCTGTCATGGTC               <  SRR4302540.5544267/94‑1 (MQ=60)
                                                                                 AGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGTGAGCCTGTCATGGTCGA             >  SRR4302540.876018/1‑94 (MQ=60)
                                                                                 AGGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGTGAGCCTGTCATGGTCGA             >  SRR4302540.4792383/1‑94 (MQ=60)
                                                                                  AGAGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGTGAGCCTGTCATAGTCGAT            <  SRR4302540.480690/94‑1 (MQ=60)
                                                                                    AGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGTGAGCCTGTCATGGTCGATAC          >  SRR4302540.2336867/1‑94 (MQ=60)
                                                                                    AGTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGTGAGCCTGTCATGGTCGATAC          <  SRR4302540.9546997/94‑1 (MQ=60)
                                                                                     GTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGTGAGCCTGTCATGGTCGATACA         <  SRR4302540.9932562/94‑1 (MQ=60)
                                                                                     GTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGTGAGCCTGTCATGGTCGATACA         <  SRR4302540.7278103/94‑1 (MQ=60)
                                                                                     GTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGTGAGCCTGTCATGGTCGATACA         <  SRR4302540.6185780/94‑1 (MQ=60)
                                                                                     GTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGTGAGCCTGTCATGGTCGATACA         >  SRR4302540.3900654/1‑94 (MQ=60)
                                                                                     GTTTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGTGAGCCTGTCATGGTCGATACA         <  SRR4302540.8995841/94‑1 (MQ=60)
                                                                                       TTCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGTGAGCCTGTCATGGTCGATACAGC       >  SRR4302540.3910367/1‑94 (MQ=60)
                                                                                        TCTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGTGAGCCTGTCATGGTCGATACAGCC      <  SRR4302540.3007757/94‑1 (MQ=60)
                                                                                         CTTGCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGTGAGCCTGTCATGGTCGATACAGCCC     >  SRR4302540.3681522/1‑94 (MQ=60)
                                                                                            GCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGTGAGCCTGTCATGGTCGATACAGCCCAGG  <  SRR4302540.3691098/94‑1 (MQ=60)
                                                                                            GCCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGTGAGCCTGTCATGGTCGATACAGCCCAGG  >  SRR4302540.9864882/1‑94 (MQ=60)
                                                                                            |                                                                                             
CGAAAGGACGGCATACCATGAATATATTCGATCACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTACCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGTGAGCCTGTCATGGTCGATACAGCCCAGG  >  NC_000913/1864915‑1865100

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑