Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1548 369.5 20095359 96.9% 19472402 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,264,802 T→C F180L (TTT→CTT)  yeiQ → putative oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,264,8020TC100.0% 798.2 / NA 246F180L (TTT→CTT) yeiQputative oxidoreductase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (130/116);  total (130/116)

Only 100 of 253 total aligned reads displayed.

CACGATCTACAAACCCCTGAAGAACCTCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGTTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGTGCTGGGAATGGCAGAAAAACGTTCGCCAGAA  >  NC_000913/2264712‑2264891
                                                                                          |                                                                                         
cACGATCTACAAACCCCTGAAGAACCTCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGctt                                                                                         <  2:7491580/93‑1 (MQ=255)
  cGATCTACAAACCCCTGAAGAACCTCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTAc                                                                                       >  2:1159867/1‑93 (MQ=255)
   gATCTACAAACCCCTGAAGAACCTCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTAcc                                                                                      >  1:1619221/1‑93 (MQ=255)
   gATCTACAAACCCCTGAAGAACCTCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTAcc                                                                                      >  2:731499/1‑93 (MQ=255)
     tCTACAAACCCCTGAAGAACCTCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCgg                                                                                    >  2:8170438/1‑92 (MQ=255)
      cTACAAACCCCTGAAGAACCTCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTg                                                                                   >  2:4233745/1‑93 (MQ=255)
       tACAAACCCCTGAAGAACCTCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGc                                                                                  <  1:8746419/93‑1 (MQ=255)
       tACAAACCCCTGAAGAACCTCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGc                                                                                  <  2:8738791/93‑1 (MQ=255)
       tACAAACCCCTGAAGAACCTCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGc                                                                                  <  2:7698427/93‑1 (MQ=255)
           aaCCCCTGAAGAACCTCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCtctc                                                                              >  1:8697795/1‑93 (MQ=255)
           aaCCCCTGAAGAACCTCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCtctc                                                                              <  1:142355/93‑1 (MQ=255)
           aaCCCCTGAAGAACCTCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCtctc                                                                              <  2:9055284/93‑1 (MQ=255)
           aaCCCCTGAAGAACCTCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCtctc                                                                              <  1:4473793/93‑1 (MQ=255)
            aCCCCTGAAGAGCATCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACCCCGCTTACCGTGCTCTcc                                                                             <  1:10028671/93‑1 (MQ=255)
            aCCCCTGAAGAACCTCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTcc                                                                             <  2:8546954/93‑1 (MQ=255)
               ccTGAAGAACCTCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGc                                                                          <  2:2516318/93‑1 (MQ=255)
                cTGAAGAACCTCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCg                                                                         <  1:6620960/93‑1 (MQ=255)
                cTGAAGAACCTCACCCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCg                                                                         <  2:2574833/93‑1 (MQ=255)
                 tGAAGAACCTCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGa                                                                        >  2:512654/1‑93 (MQ=255)
                 tGAAGAACCTCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGa                                                                        >  2:610392/1‑93 (MQ=255)
                  gaagaaCCTCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGAc                                                                       >  1:3836402/1‑93 (MQ=255)
                    agaaCCTCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACaa                                                                     >  1:6036382/1‑93 (MQ=255)
                     gaaCCTCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAat                                                                    >  1:9493441/1‑93 (MQ=255)
                     gaaCCTCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAat                                                                    >  1:5405804/1‑93 (MQ=255)
                     gaaCCTCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAat                                                                    >  2:9746531/1‑93 (MQ=255)
                      aaCCTCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAata                                                                   >  2:2234215/1‑93 (MQ=255)
                       aCCTCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAatat                                                                  >  1:1779144/1‑93 (MQ=255)
                        ccTCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAACCGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATAtt                                                                 <  2:1916368/93‑1 (MQ=255)
                        ccTCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATAtt                                                                 <  2:1700358/93‑1 (MQ=255)
                        ccTCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATAtt                                                                 <  2:6646665/93‑1 (MQ=255)
                        ccTCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATAtt                                                                 <  1:6165743/93‑1 (MQ=255)
                         cTCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTc                                                                <  2:4356850/93‑1 (MQ=255)
                          tCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTcc                                                               >  2:4643481/1‑93 (MQ=255)
                          tCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTcc                                                               <  1:8178572/93‑1 (MQ=255)
                          tCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTcc                                                               >  2:7858572/1‑93 (MQ=255)
                            ccTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCg                                                             <  2:563822/92‑1 (MQ=255)
                            aCTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCg                                                             <  2:6369347/93‑1 (MQ=255)
                              cccGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGAc                                                           <  2:397656/92‑1 (MQ=255)
                                cGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACaa                                                         <  1:7830734/93‑1 (MQ=255)
                                cGCACCGGGTATTCTCGTAGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGCCaa                                                         >  2:7854729/1‑93 (MQ=255)
                                 gCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAAt                                                        >  1:4109821/1‑93 (MQ=255)
                                  cACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATg                                                       <  2:8437272/93‑1 (MQ=255)
                                   aCCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATgg                                                      >  2:2938339/1‑93 (MQ=255)
                                   aCCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATgg                                                      >  2:2022396/1‑93 (MQ=255)
                                     cGGGTATTCTCGTCGACGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTc                                                    <  1:8923211/93‑1 (MQ=255)
                                      gggTATTCTCGTCGAAGCACTGAGACGCCGCCGTGAGCGCGGCCTTACACCGCTTTCCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCa                                                   <  2:6935207/93‑1 (MQ=255)
                                      gggTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCa                                                   <  2:5955867/93‑1 (MQ=255)
                                      gggTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCa                                                   >  2:5993168/1‑93 (MQ=255)
                                       ggTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCAt                                                  >  2:2388919/1‑93 (MQ=255)
                                        gTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCAtg                                                 >  1:8458387/1‑93 (MQ=255)
                                            tctcGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATgtggt                                             <  1:3006500/93‑1 (MQ=255)
                                                 tcgAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGaaaa                                        >  1:1463834/1‑93 (MQ=255)
                                                 tcgAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGaaaa                                        >  2:2886015/1‑93 (MQ=255)
                                                 tcgAAGCACGGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGaaaa                                        <  1:8128540/93‑1 (MQ=255)
                                                 gcgAAGCCCTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGaaaa                                        <  1:7532214/92‑1 (MQ=255)
                                                   gAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAAc                                      >  2:806638/1‑93 (MQ=255)
                                                   gAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAAc                                      >  2:8628625/1‑93 (MQ=255)
                                                   gAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAAc                                      >  1:7303721/1‑93 (MQ=255)
                                                   gAAGCACGGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAAc                                      <  1:4617483/93‑1 (MQ=255)
                                                     aGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAAcgc                                    <  2:9883739/93‑1 (MQ=255)
                                                      gCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAAcgcg                                   >  2:8124841/1‑93 (MQ=255)
                                                      gCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAAcgcg                                   >  2:2104864/1‑93 (MQ=255)
                                                      gCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAAcgcg                                   >  2:1395031/1‑93 (MQ=255)
                                                      gCACTGAAACCCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAAcgcg                                   <  1:6954392/93‑1 (MQ=255)
                                                       cACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCgg                                  >  1:5075874/1‑93 (MQ=255)
                                                        aCTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGt                                 <  1:7652148/93‑1 (MQ=255)
                                                          tGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGTGc                               >  1:6663402/1‑93 (MQ=255)
                                                           gAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGTGCt                              <  1:3456093/93‑1 (MQ=255)
                                                             aaCGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGTGCTgg                            >  1:9971460/1‑93 (MQ=255)
                                                              aCGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGTGCTggg                           <  2:7213441/93‑1 (MQ=255)
                                                              aCGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGTGCTggg                           <  1:2546595/93‑1 (MQ=255)
                                                                gccgccGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGTGCTGGGaa                         <  1:610392/93‑1 (MQ=255)
                                                                gccgccGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGTGCTGGGaa                         <  2:6480836/93‑1 (MQ=255)
                                                                 ccgccgTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGTGCTGGGAAt                        >  2:3289341/1‑93 (MQ=255)
                                                                 ccgccgTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGTGCTGGGAAt                        <  2:626397/93‑1 (MQ=255)
                                                                  cgccgTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGTGCTGGGAATg                       >  2:5205216/1‑93 (MQ=255)
                                                                   gccgTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGTGCTGGGAATgg                      >  1:6270381/1‑93 (MQ=255)
                                                                     cgTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGTGCTGGGAATGGCa                    >  2:3154617/1‑93 (MQ=255)
                                                                      gTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGTGCTGGGAATGGCAg                   >  1:3163087/1‑93 (MQ=255)
                                                                       tGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGTGCTGGGAATGGCAGa                  <  1:4379269/93‑1 (MQ=255)
                                                                       tGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGTGCTGGGAATGGCAGa                  <  2:5436470/93‑1 (MQ=255)
                                                                        gAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGTGCTGGGAATGGCAGaa                 <  1:5669361/93‑1 (MQ=255)
                                                                          gcgcGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGTGCTGGGAATGGCAGaaaa               <  2:9716144/93‑1 (MQ=255)
                                                                            gcgGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGTGCTGGGAATGGCAGAAAAAc             >  1:4289101/1‑93 (MQ=255)
                                                                            gcgGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGTGCTGGGAATGGCAGAAAAAc             >  1:1983734/1‑93 (MQ=255)
                                                                            gcgGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGTGCTGGGAATGGCAGAAAAAc             >  1:106197/1‑93 (MQ=255)
                                                                            gcgGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGTGCTGGGAATGGCAGAAAAAc             <  1:6056658/93‑1 (MQ=255)
                                                                            gcgGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGTGCTGGGAATGGCAGAAAAAc             >  1:9240539/1‑93 (MQ=255)
                                                                             cgGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGTGCTGGGAATGGCAGAAAAACg            >  2:4463689/1‑93 (MQ=255)
                                                                              ggCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGTGCTGGGAATGGCAGAAAAACGt           <  2:4918077/93‑1 (MQ=255)
                                                                              ggCCTTACACCGCTTACCGTGCTCTCCTGCGACAAGATTCCCGACAATGGTCATGTGGTGAAAAACGCGGTGCGGGGAATGGCAGAAAAACGt           >  2:4504980/1‑93 (MQ=255)
                                                                               gCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGTGCTGGGAATGGCAGAAAAACGtt          >  2:587576/1‑93 (MQ=255)
                                                                               gCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGTGCTGGGAATGGCAGAAAAACGtt          >  2:9198014/1‑93 (MQ=255)
                                                                               gCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGGGCTGGGAATGGCAGAAAAACgat          >  2:3670057/1‑91 (MQ=255)
                                                                                ccTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGTGCTGGGAATGGCAGAAAAACGTTc         >  2:6779059/1‑93 (MQ=255)
                                                                                ccTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGTGCTGGGAATGGCAGAAAAACGTTc         <  1:824984/93‑1 (MQ=255)
                                                                                 cTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGTGCTGGGAATGGCAGAAAAACGTTCg        <  2:7948923/93‑1 (MQ=255)
                                                                                  ttACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGTGCTGGGAATGGCAGAAAAACGTTCGc       >  1:92028/1‑93 (MQ=255)
                                                                                  ttACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGTGCTGGGAATGGCAGAAAAACGTTCGc       >  2:244537/1‑93 (MQ=255)
                                                                                      acCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGTGCTGGGAATGGCAGAAAAACGTTCGCCAGa   <  1:6990825/93‑1 (MQ=255)
                                                                                          |                                                                                         
CACGATCTACAAACCCCTGAAGAACCTCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGTTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGTGCTGGGAATGGCAGAAAAACGTTCGCCAGAA  >  NC_000913/2264712‑2264891

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ATTCACGATCTACAAACCCCTGAAGAACCTCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGTTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGTGCTGGGAATGGCAGAAAAACGTTCGCCAGAACTCG  >  NC_000913/2264709‑2264895
                                                                                             |                                                                                             
ATTCACGATCTACAAACCCCTGAAGAACCTCACTCCGCACCGGGTATTCTCGTCGAGGAACTGAGACGCCGCCGTGAGCGCGGCCTTACACCGC                                                                                               >  SRR4302540.4197647/1‑94 (MQ=60)
ATTCACGATCTACAAACCCCTGAAGAACCTCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGC                                                                                               >  SRR4302540.4197648/1‑94 (MQ=60)
 TTCACGATCTACAAACCCCTGAAGAACCTCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCT                                                                                              >  SRR4302540.3676965/1‑94 (MQ=60)
 TTCACGATCTACAAACCCCTGAAGAACCTCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCT                                                                                              >  SRR4302540.6813127/1‑94 (MQ=60)
 TTCACGATCTACAAACCCCTGAAGAACCTCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCT                                                                                              <  SRR4302540.2885274/94‑1 (MQ=60)
 TTCACGATCTACAAACCCCTGAAGAACCTCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCT                                                                                              >  SRR4302540.891569/1‑94 (MQ=60)
  TCACGATCTACAAACCCCTGAAGAACCTCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTT                                                                                             >  SRR4302540.4427036/1‑94 (MQ=60)
    ACGATCTACAACCCCCTGAAGAACCTCACTCCGCACCGGTTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTAC                                                                                           <  SRR4302540.4383174/94‑1 (MQ=60)
    ACGATCTACAAACCCCTGAAGAACCTCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTAC                                                                                           <  SRR4302540.4383175/94‑1 (MQ=60)
     CGATCTACAAACCCCTGAAGAACCTCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACC                                                                                          >  SRR4302540.1636979/1‑94 (MQ=60)
          TACAAACCCCTGAAGAACCTCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCT                                                                                     <  SRR4302540.7499676/94‑1 (MQ=60)
          TACAAACCCCTGAAGAACCTCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCT                                                                                     <  SRR4302540.8847916/94‑1 (MQ=60)
          TACAAACCCCTGAAGAACCTCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCT                                                                                     <  SRR4302540.1053190/94‑1 (MQ=60)
            CAAACCCCTGAAGAACCTCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCT                                                                                   >  SRR4302540.3295377/1‑94 (MQ=60)
             AAACCCCTGAAGAACCTCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTC                                                                                  >  SRR4302540.8798763/1‑94 (MQ=60)
             AAACCCCTGAAGAACCTCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTC                                                                                  >  SRR4302540.4638495/1‑94 (MQ=60)
             AAACCCCTGAAGAACCTCACTCCGCACCGGGGATTCTCGTCGAAGCCCTGAAACGCCGCCGTGAGCGCGGCCTTACACCGTTTACCGTGCTCTC                                                                                  >  SRR4302540.7623782/1‑94 (MQ=60)
              AACCCCTGAAGAACCTCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCC                                                                                 <  SRR4302540.4522317/94‑1 (MQ=60)
              AACCCCTGAAGAACCTCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCC                                                                                 <  SRR4302540.143839/94‑1 (MQ=60)
               ACCCCTGAAGAGCATCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACCCCGCTTACCGTGCTCTCCT                                                                                <  SRR4302540.10145346/94‑1 (MQ=60)
               ACCCCTGAAGAACCTCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCT                                                                                <  SRR4302540.2007310/94‑1 (MQ=60)
                 CCCTGAAGAACCTCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGC                                                                              <  SRR4302540.8898682/94‑1 (MQ=60)
                  CCTGAAGAACCTCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCG                                                                             >  SRR4302540.6817559/1‑94 (MQ=60)
                   CTGAAGAACCTCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGA                                                                            <  SRR4302540.6694274/94‑1 (MQ=60)
                    TGAAGAACCTCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGAC                                                                           >  SRR4302540.3878135/1‑94 (MQ=60)
                      AAGAACCTCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAA                                                                         >  SRR4302540.6103392/1‑94 (MQ=60)
                      AAGAACCTCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAA                                                                         >  SRR4302540.5942463/1‑94 (MQ=60)
                      AAGAACCTCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAA                                                                         >  SRR4302540.6103391/1‑94 (MQ=60)
                      AAGAACCTCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAA                                                                         >  SRR4302540.8701705/1‑94 (MQ=60)
                       AGAACCTCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAAT                                                                        >  SRR4302540.5465244/1‑94 (MQ=60)
                       AGAACCTCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAAT                                                                        >  SRR4302540.9605964/1‑94 (MQ=60)
                        GAACCTCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATA                                                                       <  SRR4302540.8077851/94‑1 (MQ=60)
                        GAACCTCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATA                                                                       >  SRR4302540.6711592/1‑94 (MQ=60)
                         aaccccccctCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATAT                                                                      <  SRR4302540.4829491/84‑1 (MQ=60)
                         AACCTCCCGCCGCACCGGGTATGCTCGGCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATAT                                                                      <  SRR4302540.7685545/94‑1 (MQ=60)
                         AACCTCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATAT                                                                      <  SRR4302540.3084249/94‑1 (MQ=60)
                         AACCTCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATAT                                                                      >  SRR4302540.1798460/1‑94 (MQ=60)
                          ACCTCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATT                                                                     <  SRR4302540.8544790/94‑1 (MQ=60)
                           CCTCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTC                                                                    <  SRR4302540.6234444/94‑1 (MQ=60)
                            CTCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCC                                                                   >  SRR4302540.7354318/1‑94 (MQ=60)
                             TCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCC                                                                  >  SRR4302540.8216495/1‑94 (MQ=60)
                             TCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCC                                                                  <  SRR4302540.8273569/94‑1 (MQ=60)
                              CACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCG                                                                 >  SRR4302540.1887677/1‑94 (MQ=60)
                               CCTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGA                                                                <  SRR4302540.6577236/94‑1 (MQ=60)
                                CTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGAC                                                               >  SRR4302540.1776385/1‑94 (MQ=60)
                                CTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCATGCTCTCCTGCGACAATATTCCCGAC                                                               >  SRR4302540.9154147/1‑94 (MQ=60)
                                   CGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAAT                                                            >  SRR4302540.4154185/1‑94 (MQ=60)
                                   CGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAAT                                                            <  SRR4302540.7922231/94‑1 (MQ=60)
                                    GCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATG                                                           >  SRR4302540.6113853/1‑94 (MQ=60)
                                        CGGGTATTCTCGTCGACGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCA                                                       <  SRR4302540.9026791/94‑1 (MQ=60)
                                        CGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCA                                                       >  SRR4302540.8808131/1‑94 (MQ=60)
                                         GGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCAT                                                      >  SRR4302540.8256818/1‑94 (MQ=60)
                                         GGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCAT                                                      >  SRR4302540.4284267/1‑94 (MQ=60)
                                          GGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATG                                                     >  SRR4302540.8556597/1‑94 (MQ=60)
                                             ATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGG                                                  <  SRR4302540.10095723/94‑1 (MQ=60)
                                              TTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGT                                                 >  SRR4302540.635126/1‑94 (MQ=60)
                                               TCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTG                                                <  SRR4302540.3039597/94‑1 (MQ=60)
                                               TCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTG                                                <  SRR4302540.5123466/94‑1 (MQ=60)
                                                 TCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAA                                              <  SRR4302540.2703202/94‑1 (MQ=60)
                                                   GTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAA                                            >  SRR4302540.1479799/1‑94 (MQ=60)
                                                   GTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAA                                            >  SRR4302540.83099/1‑94 (MQ=60)
                                                    TCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAGAA                                           >  SRR4302540.5311920/1‑94 (MQ=60)
                                                    TCGAAGCACGGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAA                                           <  SRR4302540.8222888/94‑1 (MQ=60)
                                                    GCGAAGCCCTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAA                                           <  SRR4302540.7616554/94‑1 (MQ=60)
                                                     CGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAAC                                          >  SRR4302540.7385249/1‑94 (MQ=60)
                                                     CGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAAC                                          >  SRR4302540.4556262/1‑94 (MQ=60)
                                                      GAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACG                                         <  SRR4302540.5112845/94‑1 (MQ=60)
                                                      GAAGCACGGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACG                                         <  SRR4302540.4667001/94‑1 (MQ=60)
                                                         GCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGG                                      >  SRR4302540.5130755/1‑94 (MQ=60)
                                                         GCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGG                                      <  SRR4302540.3990765/94‑1 (MQ=60)
                                                         GCACTGAAACCCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGG                                      <  SRR4302540.7031764/94‑1 (MQ=60)
                                                         GCACGGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGG                                      <  SRR4302540.7031763/94‑1 (MQ=60)
                                                          cactgagcgGCCGCCGTGAGCGCGGCCTGAGACCGTTTACCGTGCTCTCCTGCGAAAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGT                                     <  SRR4302540.730875/85‑1 (MQ=60)
                                                          CACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGT                                     <  SRR4302540.730876/94‑1 (MQ=60)
                                                           ACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGTG                                    <  SRR4302540.7738291/94‑1 (MQ=60)
                                                           ACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGTG                                    >  SRR4302540.6129340/1‑94 (MQ=60)
                                                            CTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGCCAATATTCCCGACAATGGTCATGTGGGGAAAAACGCGGTGT                                   >  SRR4302540.8251947/1‑94 (MQ=60)
                                                            CTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGTGC                                   >  SRR4302540.6737064/1‑94 (MQ=60)
                                                             TGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGTGCT                                  <  SRR4302540.8443244/94‑1 (MQ=60)
                                                              GAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGTGCTG                                 <  SRR4302540.3493708/94‑1 (MQ=60)
                                                               AAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGTGCTGG                                >  SRR4302540.10087774/1‑94 (MQ=60)
                                                                ACCGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTCCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGTGCTGGG                               <  SRR4302540.9156377/94‑1 (MQ=60)
                                                                 ACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGTGCTGGGA                              <  SRR4302540.2574043/94‑1 (MQ=60)
                                                                   GCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGTGCTGGGAAT                            <  SRR4302540.616916/94‑1 (MQ=60)
                                                                   GCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGTGCTGGGAAT                            <  SRR4302540.5576907/94‑1 (MQ=60)
                                                                   CCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGTGCTGGGAAT                            <  SRR4302540.1940256/94‑1 (MQ=60)
                                                                    CCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGTGCTGGGAATG                           <  SRR4302540.9603831/94‑1 (MQ=60)
                                                                     CGCCGTGAGGGCGGCCTTACACCGATTACAGTGCTCTCCTGCGACAATATTCCCTCCAATGGTCAGGTGGTGAAAgaccggctgctgcgagagt                          >  SRR4302540.366770/1‑75 (MQ=54)
                                                                     CGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGTGCTGGGAATGG                          >  SRR4302540.6340145/1‑94 (MQ=60)
                                                                     CGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGTGCTGCGAATGG                          >  SRR4302540.366768/1‑94 (MQ=60)
                                                                       CCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGTGCTGGGAATGGCA                        <  SRR4302540.7820437/94‑1 (MQ=60)
                                                                        CGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGTGCTGGGAATGGCAG                       >  SRR4302540.3197492/1‑94 (MQ=60)
                                                                        CGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGTGCTGGGAATGGCAG                       >  SRR4302540.1576647/1‑94 (MQ=60)
                                                                          TGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGTGCTGGGAATGGCAGAA                     <  SRR4302540.4426817/94‑1 (MQ=60)
                                                                           GAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGTGCTGGGAATGGCAGAAA                    <  SRR4302540.880248/94‑1 (MQ=60)
                                                                           GAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGTGCTGGGAATGGCAGAAA                    <  SRR4302540.5731786/94‑1 (MQ=60)
                                                                           GAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGTGCTGGGAATGGCAGAAA                    <  SRR4302540.6322005/94‑1 (MQ=60)
                                                                              CGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGTGCTGGGAATGGCAGCACAAC                 >  SRR4302540.9351223/1‑94 (MQ=60)
                                                                              CGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGTGCTGGGAATGGCAGAAAAAC                 >  SRR4302540.2005240/1‑94 (MQ=60)
                                                                              CGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGTGCTGGGAATGGCAGAAAAAC                 >  SRR4302540.107331/1‑94 (MQ=60)
                                                                              CGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGTGCTGGGAATGGCAGAAAAAC                 >  SRR4302540.9351224/1‑94 (MQ=60)
                                                                              CGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGTGCTGGGAATGGCAGAAAAAC                 >  SRR4302540.4335760/1‑94 (MQ=60)
                                                                               GCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGTGCTGGGAATGGCAGAAAAACG                <  SRR4302540.6123969/94‑1 (MQ=60)
                                                                                 GGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGTGCTGGGAATGGCAGAAAAACGTT              >  SRR4302540.1360358/1‑94 (MQ=60)
                                                                                  GCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGTGCTGGGAATGGCAGAAAAACGTTC             <  SRR4302540.2979422/94‑1 (MQ=60)
                                                                                  GCCTTACACCGCTTACCGTGCTCTCCGGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGTGCTGGGAATGGCAGAAAAACGTTC             <  SRR4302540.9976406/94‑1 (MQ=60)
                                                                                   CCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGTGCTGGGAATGGCAGAAAAACGTTCG            <  SRR4302540.833761/94‑1 (MQ=60)
                                                                                   CCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGTGCTGGGAATGGCAGAAAAACGTTCG            >  SRR4302540.3980576/1‑94 (MQ=60)
                                                                                   CCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGTGCTGGGAATGGCAGAAAAACGTTCG            <  SRR4302540.3290933/94‑1 (MQ=60)
                                                                                    CTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGTGCTGGGATTGGCAGAAAAACGTTCGC           >  SRR4302540.6193718/1‑94 (MQ=60)
                                                                                    CTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGTGCTGGGAATGGCAGACAAACGTTCGC           >  SRR4302540.6193719/1‑94 (MQ=60)
                                                                                    CTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGTGCTGGGAATGGCAGAAAAACGTTCGC           >  SRR4302540.92998/1‑94 (MQ=60)
                                                                                    CTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGTGCTGGGAATGGCAGAAAAACGTTCGC           <  SRR4302540.9446006/94‑1 (MQ=60)
                                                                                     TTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGTGCTGGGAATGGCAGAAAAACGTTCGCC          >  SRR4302540.1569251/1‑94 (MQ=60)
                                                                                     TTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGTGCTGGGAATGGCAGAAAAACGTTCGCC          <  SRR4302540.1131178/94‑1 (MQ=60)
                                                                                        CACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGTGCTGGGAATGGCAGAAAAACGTTCGCCAGA       >  SRR4302540.7050865/1‑94 (MQ=60)
                                                                                        CACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGTGCTGGGAATGGCAGAAAAACGTTCGCCAGA       <  SRR4302540.147284/94‑1 (MQ=60)
                                                                                         ACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGTGCTGGGAATGGCAGAAAAACGTTCGCCAGAA      >  SRR4302540.5632049/1‑94 (MQ=60)
                                                                                         ACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGTGCTGGGAATGGCAGAAAAACGTTCGCCAGAA      <  SRR4302540.7068425/94‑1 (MQ=60)
                                                                                          CCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGTGCTGGGAATGGCAGAAAAACGTTCGCCAGAAC     >  SRR4302540.8769347/1‑94 (MQ=60)
                                                                                           CGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGTGCTGGGAATGGCAGAAAAACGTTCGCCAGAACT    >  SRR4302540.329463/1‑94 (MQ=60)
                                                                                           CGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGTGCTGGGAATGGCAGAAAAACGTTCGCCAGAACT    >  SRR4302540.958672/1‑94 (MQ=60)
                                                                                            GCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGTGCTGGGAATGGCAGAAAAACGTTCGCCAGAACTC   <  SRR4302540.5248623/94‑1 (MQ=60)
                                                                                            GCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGTGCTGGGAATGGCAGAAAAACGTTCGCCAGAACTC   <  SRR4302540.5033668/94‑1 (MQ=60)
                                                                                            GCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGTGCTGGGAATGGCAGAAAAACGTTCGCCAGAACTC   >  SRR4302540.4199263/1‑94 (MQ=60)
                                                                                             CTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGTGCTGGGAATGGCAGAAAAACGTTCGCCAGAACTCG  <  SRR4302540.7719112/94‑1 (MQ=60)
                                                                                             |                                                                                             
ATTCACGATCTACAAACCCCTGAAGAACCTCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGTTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGTGCTGGGAATGGCAGAAAAACGTTCGCCAGAACTCG  >  NC_000913/2264709‑2264895

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑