Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1548 369.5 20095359 96.9% 19472402 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,766,423 G→A R231R (CGG→CGA)  b2632 → putative GTP‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,766,4230GA99.6% 877.8 / NA 251R231R (CGG→CGA) b2632putative GTP‑binding protein
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base A (132/118);  total (133/118)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 259 total aligned reads displayed.

GGGACTAAGGGTGATGGCAGAGCGGATGATTAAGTGTCTGCCGCGTGAGCCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGGACATGGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGATACCATACTCGATACGGTAAGCACCTTTCCCCTTATTC  >  NC_000913/2766333‑2766511
                                                                                          |                                                                                        
gggACTAAGGGTGATGGCAGAGCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAAc                                                                                        >  2:2228233/1‑93 (MQ=255)
   aCTAAGGGTGATGGCAGAGCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACaac                                                                                     <  2:7848522/93‑3 (MQ=255)
   aCTAAGGGTGATGGCAGAGCGGATGATTAAGTGTCTGCCGCGGGAGGCCAGCAGCCCGGCAGTACCGCTACTGCAACATCCCTTTCGAAccag                                                                                     >  1:4538324/1‑90 (MQ=25)
     tAAGGGTGATGGCAGAGCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACaacgg                                                                                   >  2:3696476/1‑89 (MQ=25)
     tAAGGGTGATGGCAGAGCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACaacgg                                                                                   >  1:6121738/1‑89 (MQ=25)
     tAAGGGTGATGGCAGAGCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACaacgg                                                                                   >  2:6872208/1‑89 (MQ=25)
       aGGGTGATGGCAGAGCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTa                                                                                 >  1:8901068/1‑93 (MQ=25)
       aGGGTGATGGCAGAGCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTa                                                                                 <  2:6632763/93‑1 (MQ=25)
         ggTGATGGCAGAGCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGc                                                                               <  1:8268595/93‑1 (MQ=25)
         ggTGATGGCAGAGCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGc                                                                               <  2:8042130/93‑1 (MQ=21)
          gTGATGGCAGAGCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGcc                                                                              <  1:141855/93‑1 (MQ=25)
          gTGATGGCAGAGCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGcc                                                                              <  1:3871371/93‑1 (MQ=25)
           tGATGGCAGAGCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGccc                                                                             <  1:7066938/93‑1 (MQ=25)
           tGATGGCAGAGCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGccc                                                                             <  2:3874339/93‑1 (MQ=21)
            gATGGCAGAGCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGAAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCg                                                                            >  1:3042860/1‑93 (MQ=21)
             aTGGCAGAGCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCgg                                                                           <  2:8704021/93‑1 (MQ=25)
              tGGCAGAGCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCggg                                                                          <  1:3550729/93‑1 (MQ=25)
                  agagCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCa                                                                      >  2:2144892/1‑93 (MQ=25)
                  agagCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCa                                                                      >  1:5253625/1‑93 (MQ=25)
                  agagCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCa                                                                      <  1:5881214/93‑1 (MQ=25)
                     gCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGc                                                                   <  1:8097040/93‑1 (MQ=25)
                       ggATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCAc                                                                 >  1:6970805/1‑93 (MQ=255)
                        gatgatTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACg                                                                >  1:1765907/1‑93 (MQ=255)
                        gatgatTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACg                                                                >  2:4123487/1‑93 (MQ=255)
                        gatgatTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACg                                                                >  1:1544627/1‑93 (MQ=255)
                        gatgatTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACg                                                                >  2:2718457/1‑93 (MQ=255)
                        gatgatTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACg                                                                <  2:6365710/93‑1 (MQ=255)
                         atgatTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGt                                                               <  1:7593090/93‑1 (MQ=255)
                          tgatTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTg                                                              <  2:519629/93‑1 (MQ=255)
                           gatTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGGACAACGGTAGCCCGGGAGCAGGCACGTGa                                                             >  2:2086193/1‑93 (MQ=255)
                           gatTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCACGCACGTGa                                                             >  1:9183728/1‑93 (MQ=255)
                              tAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGa                                                          <  1:1317787/93‑1 (MQ=255)
                              tAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGa                                                          >  2:9100303/1‑93 (MQ=255)
                                aGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGact                                                        >  1:4664537/1‑91 (MQ=255)
                                aGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGAtt                                                        >  2:166969/1‑93 (MQ=255)
                                       gCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGa                                                 >  1:8570993/1‑93 (MQ=255)
                                       gCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGa                                                 >  2:5891057/1‑93 (MQ=255)
                                       gCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGa                                                 <  1:3106809/93‑1 (MQ=255)
                                       gCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGa                                                 <  1:2937258/93‑1 (MQ=255)
                                         cgcgTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGaaa                                               <  2:544681/93‑1 (MQ=255)
                                         cgcgTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGaaa                                               >  1:1241590/1‑93 (MQ=255)
                                          gcgTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAAc                                              >  2:9779704/1‑93 (MQ=255)
                                           cgTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAAcc                                             >  1:4076514/1‑93 (MQ=255)
                                           cgTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAAcc                                             >  1:1009661/1‑93 (MQ=255)
                                            gTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCCGGAGCAGGCACGTGACGCTTTCGGTGAAACCg                                            >  2:4880987/1‑93 (MQ=255)
                                             tGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGATCAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGt                                           >  2:6002303/1‑93 (MQ=255)
                                             tGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGt                                           <  2:2534500/93‑1 (MQ=255)
                                              gAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTGCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTa                                          <  2:1180978/93‑1 (MQ=255)
                                                ggccAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAgg                                        <  1:9024842/91‑2 (MQ=255)
                                                ggccAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAgg                                        <  1:5442585/91‑2 (MQ=255)
                                                ggccAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACAGTAgg                                        >  1:104120/3‑92 (MQ=255)
                                                 gccAGCAGCCCGGTAGTAGCTCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAggt                                       <  1:6713780/92‑3 (MQ=255)
                                                 gccAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAggt                                       >  2:2997775/2‑91 (MQ=255)
                                                     gcagcCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCa                                   >  1:8774340/1‑93 (MQ=255)
                                                       agcCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATa                                 >  2:3450042/1‑93 (MQ=255)
                                                        gcCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTCGGTGCCATAc                                >  1:8412691/1‑93 (MQ=255)
                                                        gcCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATAc                                >  2:6820255/1‑93 (MQ=255)
                                                        gcCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATAc                                <  2:9536398/93‑1 (MQ=255)
                                                         cccGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACt                               <  2:4292822/93‑1 (MQ=255)
                                                         cccGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACt                               >  1:2291753/1‑93 (MQ=255)
                                                         cccGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACt                               >  1:4967073/1‑93 (MQ=255)
                                                          ccGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTc                              >  2:7108825/1‑93 (MQ=255)
                                                          ccGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTc                              >  1:7231242/1‑93 (MQ=255)
                                                           cGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTCGGTGCCATACTCg                             >  2:6759131/1‑93 (MQ=255)
                                                           cGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCg                             <  1:9457851/93‑1 (MQ=255)
                                                            ggaagtagCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCTGACTCGa                            >  2:2419440/4‑93 (MQ=255)
                                                            ggTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGa                            >  1:7651467/1‑93 (MQ=255)
                                                            ggTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGa                            <  1:9559452/93‑1 (MQ=255)
                                                            ggTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGa                            >  2:5739750/1‑93 (MQ=255)
                                                             gtagtaGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGAt                           <  1:4477322/93‑1 (MQ=255)
                                                             gtagtaGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGAt                           >  2:6238447/1‑93 (MQ=255)
                                                              tggtagCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATa                          <  1:3784028/91‑1 (MQ=255)
                                                              tagtagCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATa                          <  2:5304924/93‑1 (MQ=255)
                                                              tagtagCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATa                          <  2:1079661/93‑1 (MQ=255)
                                                               agtagCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATAc                         <  1:5709242/93‑1 (MQ=255)
                                                                 tagCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACgg                       >  1:5608103/1‑93 (MQ=255)
                                                                 tagCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACgg                       <  2:3238862/93‑1 (MQ=255)
                                                                 tagCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACgg                       >  1:7262414/1‑93 (MQ=255)
                                                                  agCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGt                      <  1:6527594/93‑1 (MQ=255)
                                                                    cgcTACTGCAACTTCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTaa                    <  2:8117755/93‑1 (MQ=255)
                                                                    cgcTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTaa                    <  1:6672334/93‑1 (MQ=255)
                                                                    cgcTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTaa                    <  1:548467/93‑1 (MQ=255)
                                                                     gcTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAg                   >  2:7418363/1‑93 (MQ=255)
                                                                      cTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGc                  <  2:9419296/93‑1 (MQ=255)
                                                                        aCTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCAc                <  1:981251/93‑1 (MQ=255)
                                                                          tGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCt              >  1:2783220/1‑93 (MQ=255)
                                                                          tGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGAAATCACCt              >  1:6244604/1‑93 (MQ=255)
                                                                           gCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCtt             <  1:2157923/93‑1 (MQ=255)
                                                                           gCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCtt             >  2:6286187/1‑93 (MQ=255)
                                                                           gCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCtt             >  2:3344366/1‑93 (MQ=255)
                                                                             aaCATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTc           >  1:2422338/1‑93 (MQ=255)
                                                                              aCATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTcc          <  2:4408356/93‑1 (MQ=255)
                                                                              aCATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTcc          >  2:9201403/1‑93 (MQ=255)
                                                                              aCATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTcc          >  1:7487214/1‑93 (MQ=255)
                                                                               cATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTccc         <  2:1046606/93‑1 (MQ=255)
                                                                                aTCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTcccc        >  1:7369363/1‑93 (MQ=255)
                                                                                 tCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCTCCTTTCCCCt       <  2:527271/93‑1 (MQ=255)
                                                                                 tCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCt       >  2:8395484/1‑93 (MQ=255)
                                                                                    cTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTAt    <  1:3348896/93‑1 (MQ=255)
                                                                                      ttCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTc  <  2:306947/93‑1 (MQ=255)
                                                                                          |                                                                                        
GGGACTAAGGGTGATGGCAGAGCGGATGATTAAGTGTCTGCCGCGTGAGCCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGGACATGGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGATACCATACTCGATACGGTAAGCACCTTTCCCCTTATTC  >  NC_000913/2766333‑2766511

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GTGGGGACTAAGGGTGATGGCAGAGCGGATGATTAAGTGTCTGCCGCGTGAGCCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGGACATGGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGATACCATACTCGATACGGTAAGCACCTTTCCCCTTATT  >  NC_000913/2766330‑2766510
                                                                                             |                                                                                       
GTGGGGACTAAGGGCGATGGCAGAGCGGATGATTAAGTGTCTGCCGCGTGAGGCAAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGA                                                                                         >  SRR4302540.8351298/1‑94 (MQ=60)
 TGGGGACTAAGGGTGATGGCAGAGCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAA                                                                                        >  SRR4302540.9327049/1‑94 (MQ=60)
  GGGGACTAAGGGTGATGGCAGAGCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAAC                                                                                       <  SRR4302540.8711148/94‑1 (MQ=60)
  GGGGACTAAGGGTGATGGCAGAGCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAAC                                                                                       <  SRR4302540.1055289/94‑1 (MQ=60)
   GGGACTAAGGGTGATGGCAGAGCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACA                                                                                      <  SRR4302540.5673275/94‑1 (MQ=60)
   GGGACTAAGGGTGATGGCAGAGCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACA                                                                                      <  SRR4302540.2818551/94‑1 (MQ=60)
          AGGGTGATGGCAGAGCGGATGATTAAGTGTCCGCGGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCACCATCCCTTTCGAACAACGGTAG                                                                               <  SRR4302540.3906930/94‑1 (MQ=60)
            GGTGATGGCAGAGCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCC                                                                             >  SRR4302540.90053/1‑94 (MQ=60)
            GGTGATGGCAGAGCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCC                                                                             <  SRR4302540.8364671/94‑1 (MQ=60)
             GTGATGGCAGAGCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCC                                                                            <  SRR4302540.3913456/94‑1 (MQ=60)
             GTGATGGCAGAGCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCC                                                                            <  SRR4302540.143329/94‑1 (MQ=60)
             GTGATGGCAGAGCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCC                                                                            >  SRR4302540.7559792/1‑94 (MQ=60)
             GTGATGGCAGAGCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCC                                                                            <  SRR4302540.1905675/94‑1 (MQ=60)
              TGATGGCAGAGCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCG                                                                           <  SRR4302540.7145535/94‑1 (MQ=60)
              TGATGGCAGAGCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCG                                                                           <  SRR4302540.5589111/94‑1 (MQ=60)
              TGATGGCAGAGCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGAAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCG                                                                           >  SRR4302540.3076252/1‑94 (MQ=60)
                 TGGCAGAGCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGA                                                                        <  SRR4302540.3589523/94‑1 (MQ=60)
                    CAGAGCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCA                                                                     >  SRR4302540.5310929/1‑94 (MQ=60)
                     AGAGCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAG                                                                    <  SRR4302540.5946213/94‑1 (MQ=60)
                     AGAGCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAG                                                                    >  SRR4302540.7391304/1‑94 (MQ=60)
                     AGAGCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAG                                                                    <  SRR4302540.6656034/94‑1 (MQ=60)
                     AGAGCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAG                                                                    <  SRR4302540.1538106/94‑1 (MQ=60)
                      GAGCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGG                                                                   <  SRR4302540.1713573/94‑1 (MQ=60)
                      GAGCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGG                                                                   <  SRR4302540.9612681/94‑1 (MQ=60)
                       AGCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGC                                                                  <  SRR4302540.6303155/94‑1 (MQ=60)
                        GCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCA                                                                 <  SRR4302540.8191025/94‑1 (MQ=60)
                         CGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCAC                                                                >  SRR4302540.7048288/1‑94 (MQ=60)
                         CGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCAC                                                                <  SRR4302540.1143503/94‑1 (MQ=60)
                         CGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCAC                                                                <  SRR4302540.4270176/94‑1 (MQ=60)
                          GGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACG                                                               >  SRR4302540.3984048/1‑94 (MQ=60)
                          GGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACG                                                               >  SRR4302540.1785058/1‑94 (MQ=60)
                          GGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACG                                                               >  SRR4302540.1561680/1‑94 (MQ=60)
                          GGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACG                                                               >  SRR4302540.5627716/1‑94 (MQ=60)
                            ATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTG                                                             <  SRR4302540.7678395/94‑1 (MQ=60)
                            ATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTG                                                             >  SRR4302540.3025067/1‑94 (MQ=60)
                             TGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGA                                                            >  SRR4302540.9428130/1‑94 (MQ=60)
                             TGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCACGCACGTGA                                                            >  SRR4302540.9293868/1‑94 (MQ=60)
                               ATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACG                                                          >  SRR4302540.3047227/1‑94 (MQ=60)
                               ATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACACCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACG                                                          <  SRR4302540.3195540/94‑1 (MQ=60)
                                TTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGA                                                         >  SRR4302540.8366748/1‑94 (MQ=60)
                                 TAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGAT                                                        <  SRR4302540.6330565/94‑1 (MQ=60)
                                 TAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGAT                                                        <  SRR4302540.1331503/94‑1 (MQ=60)
                                  AAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATT                                                       >  SRR4302540.5214362/1‑94 (MQ=60)
                                  AAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATT                                                       >  SRR4302540.3588318/1‑94 (MQ=60)
                                  AAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATT                                                       >  SRR4302540.4714472/1‑94 (MQ=60)
                                  AAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGACT                                                       >  SRR4302540.4714471/1‑94 (MQ=60)
                                   AGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTT                                                      >  SRR4302540.5497651/1‑94 (MQ=60)
                                     TGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCG                                                    >  SRR4302540.8735640/1‑94 (MQ=60)
                                        CTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTG                                                 >  SRR4302540.5154263/1‑94 (MQ=60)
                                         TGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGA                                                <  SRR4302540.6107514/94‑1 (MQ=60)
                                         TGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGA                                                >  SRR4302540.8670565/1‑94 (MQ=60)
                                         TGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGA                                                <  SRR4302540.6107515/94‑1 (MQ=60)
                                          GCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAA                                               <  SRR4302540.2969495/94‑1 (MQ=60)
                                          GCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAA                                               <  SRR4302540.3140816/94‑1 (MQ=60)
                                           CCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAA                                              >  SRR4302540.1254260/1‑94 (MQ=60)
                                           CCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAA                                              >  SRR4302540.4650870/1‑94 (MQ=60)
                                            CGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAAC                                             <  SRR4302540.9130646/94‑1 (MQ=60)
                                            CGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTACCCCGGTAGCAGCAACGTGAGGATTTCGTTGAAAC                                             >  SRR4302540.8271156/1‑94 (MQ=42)
                                             GCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACC                                            >  SRR4302540.4120458/1‑94 (MQ=60)
                                             GCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACC                                            >  SRR4302540.1019898/1‑94 (MQ=60)
                                             GCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACC                                            <  SRR4302540.9694308/94‑1 (MQ=60)
                                             GCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACC                                            <  SRR4302540.3847954/94‑1 (MQ=60)
                                              CGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCG                                           >  SRR4302540.3621513/1‑94 (MQ=60)
                                              CGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCG                                           >  SRR4302540.7179168/1‑94 (MQ=60)
                                               GTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGT                                          >  SRR4302540.8919515/1‑94 (MQ=60)
                                               GTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGT                                          >  SRR4302540.6860957/1‑94 (MQ=60)
                                                  AGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGG                                       >  SRR4302540.105227/1‑94 (MQ=60)
                                                  AGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACAGTAGG                                       >  SRR4302540.105228/1‑94 (MQ=60)
                                                   GGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGT                                      >  SRR4302540.1750351/1‑94 (MQ=60)
                                                   GGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGT                                      <  SRR4302540.5502471/94‑1 (MQ=60)
                                                   GGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGT                                      <  SRR4302540.9133131/94‑1 (MQ=60)
                                                    GCCAGCAGCCCGGTAGTAGCTCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGgtg                                     <  SRR4302540.6787859/94‑4 (MQ=60)
                                                     gcgGCAGCCCGTTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCATGCACGTGACGATTTCGGTGAAACCGTAGgtgc                                    <  SRR4302540.8333197/91‑5 (MQ=60)
                                                       AGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCA                                  >  SRR4302540.8875987/1‑94 (MQ=60)
                                                        GCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCAT                                 <  SRR4302540.6772042/94‑1 (MQ=60)
                                                        GCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCAT                                 <  SRR4302540.6772043/94‑1 (MQ=60)
                                                         CAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATA                                <  SRR4302540.2798958/94‑1 (MQ=60)
                                                          AGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTCGGTGCCATAC                               >  SRR4302540.8510338/1‑94 (MQ=60)
                                                          AGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATAC                               >  SRR4302540.310694/1‑94 (MQ=60)
                                                           GCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACT                              >  SRR4302540.2316418/1‑94 (MQ=60)
                                                           GCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACT                              >  SRR4302540.5020801/1‑94 (MQ=60)
                                                            CCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTC                             >  SRR4302540.7311885/1‑94 (MQ=60)
                                                            CCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTC                             >  SRR4302540.7311882/1‑94 (MQ=60)
                                                            CCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTC                             <  SRR4302540.8693716/94‑1 (MQ=60)
                                                             CCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCG                            >  SRR4302540.4538660/1‑94 (MQ=60)
                                                              CGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGA                           >  SRR4302540.7737605/1‑94 (MQ=60)
                                                              CGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGA                           >  SRR4302540.7737604/1‑94 (MQ=60)
                                                              CGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGA                           <  SRR4302540.8632487/94‑1 (MQ=60)
                                                              CGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGA                           <  SRR4302540.9570083/94‑1 (MQ=60)
                                                               GGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGAT                          >  SRR4302540.9401897/1‑94 (MQ=60)
                                                               GGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGAT                          <  SRR4302540.9672477/94‑1 (MQ=60)
                                                               GGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGAT                          <  SRR4302540.10098375/94‑1 (MQ=60)
                                                                GTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATA                         <  SRR4302540.4525889/94‑1 (MQ=60)
                                                                GTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATA                         <  SRR4302540.3221419/94‑1 (MQ=60)
                                                                 TGGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATAC                        <  SRR4302540.3825297/94‑1 (MQ=60)
                                                                 TAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATAC                        <  SRR4302540.7110457/94‑1 (MQ=60)
                                                                 TAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATAC                        >  SRR4302540.8660806/1‑94 (MQ=60)
                                                                 TAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATAC                        <  SRR4302540.3825298/94‑1 (MQ=60)
                                                                  AGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACG                       <  SRR4302540.5772093/94‑1 (MQ=60)
                                                                  AGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACG                       <  SRR4302540.3668387/94‑1 (MQ=60)
                                                                   GTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGG                      >  SRR4302540.5669804/1‑94 (MQ=60)
                                                                   GTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGG                      >  SRR4302540.7343444/1‑94 (MQ=60)
                                                                   GTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGG                      >  SRR4302540.7285470/1‑94 (MQ=60)
                                                                   GTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGG                      >  SRR4302540.10028111/1‑94 (MQ=60)
                                                                     AGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTA                    <  SRR4302540.6600084/94‑1 (MQ=60)
                                                                       CGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAG                  <  SRR4302540.554443/94‑1 (MQ=60)
                                                                       CGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAG                  <  SRR4302540.6746055/94‑1 (MQ=60)
                                                                        GCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGC                 >  SRR4302540.4224297/1‑94 (MQ=60)
                                                                          TACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCAC               >  SRR4302540.1378406/1‑94 (MQ=60)
                                                                          TACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCAC               >  SRR4302540.1378407/1‑94 (MQ=60)
                                                                          TACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCAC               <  SRR4302540.219928/94‑1 (MQ=60)
                                                                          TACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTAGATACGGTAAGCAC               >  SRR4302540.1790482/1‑94 (MQ=60)
                                                                           ACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACC              <  SRR4302540.991303/94‑1 (MQ=60)
                                                                           ACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACC              <  SRR4302540.7439725/94‑1 (MQ=60)
                                                                            CTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCT             >  SRR4302540.7639101/1‑94 (MQ=60)
                                                                            CTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCT             >  SRR4302540.5854961/1‑94 (MQ=60)
                                                                            CTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCT             >  SRR4302540.9641298/1‑94 (MQ=60)
                                                                            CTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCT             >  SRR4302540.2813283/1‑94 (MQ=60)
                                                                            CTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGAAATCACCT             >  SRR4302540.6314095/1‑94 (MQ=60)
                                                                             TGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTT            <  SRR4302540.1258060/94‑1 (MQ=60)
                                                                              GCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTT           <  SRR4302540.5429635/94‑1 (MQ=60)
                                                                              GCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTT           <  SRR4302540.2181159/94‑1 (MQ=60)
                                                                               CAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTC          >  SRR4302540.2448437/1‑94 (MQ=60)
                                                                                AACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCC         >  SRR4302540.7465652/1‑94 (MQ=60)
                                                                                AACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCC         >  SRR4302540.7570791/1‑94 (MQ=60)
                                                                                AACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCC         >  SRR4302540.6397318/1‑94 (MQ=60)
                                                                                AACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCC         >  SRR4302540.772825/1‑94 (MQ=60)
                                                                                 ACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCC        >  SRR4302540.830015/1‑94 (MQ=60)
                                                                                  CATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCC       >  SRR4302540.7451511/1‑94 (MQ=60)
                                                                                   ATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCT      >  SRR4302540.4631672/1‑94 (MQ=60)
                                                                                   ATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCT      >  SRR4302540.5535142/1‑94 (MQ=60)
                                                                                       CTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATT  <  SRR4302540.3385616/94‑1 (MQ=60)
                                                                                       CTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATT  >  SRR4302540.8480142/1‑94 (MQ=60)
                                                                                             |                                                                                       
GTGGGGACTAAGGGTGATGGCAGAGCGGATGATTAAGTGTCTGCCGCGTGAGCCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGGACATGGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGATACCATACTCGATACGGTAAGCACCTTTCCCCTTATT  >  NC_000913/2766330‑2766510

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑