Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1548 369.5 20095359 96.9% 19472402 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,026,252 2 bp→GC coding (110‑111/1164 nt) fadA ← thiolase I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,026,2520CG100.0% 1055.2 / NA 331T37S (ACG→AGC) fadAthiolase I
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (167/164);  total (167/164)
*NC_0009134,026,2530GC99.7% 1061.5 / ‑4.9 333T37S (ACG→AGC) fadAthiolase I
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base C (167/165);  minor base A (0/1);  total (167/166)
Fisher's exact test for biased strand distribution p-value = 4.98e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.69e-01

Only 100 of 347 total aligned reads displayed.

AAACCCTGCTCCAGCGTCTGCTGCACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGCGTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAACGGCCCATCGGGGTGCGAATTGCA  >  NC_000913/4026166‑4026339
                                                                                      ||                                                                                      
 aaCCCTGCTCCAGCGTCTGCTGCACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGGTACGCGCCAGCAGGCTACGCa                                                                                  >  2:9583337/1‑93 (MQ=255)
     cTGCTCCAGCGTCTGCTGCACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTaa                                                                              >  1:293773/1‑93 (MQ=255)
      tGCTCCAGCGTCTGCTGCACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTaaa                                                                             <  1:6092805/93‑1 (MQ=255)
      tGCTCCAGCGTCTGCTGCACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTaaa                                                                             <  1:2667086/93‑1 (MQ=255)
      tGCTCCAGCGTCTGCTCCACACACCCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTaaa                                                                             <  2:5374013/93‑1 (MQ=255)
       gCTCCAGCGTCTGCTGCACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCCGCGGCGGGCTACGCGCCAGCAGGCTACGCATTAAAt                                                                            >  2:9765974/1‑93 (MQ=255)
         tCCAGCGTCTGCTGCACACACCCCCAGTAAATAGCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGa                                                                          <  2:5663000/93‑1 (MQ=255)
         tCCAGCGTCTGCTGCACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGa                                                                          >  2:9524065/1‑93 (MQ=255)
         tCCAGCGTCTGCTGCACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGa                                                                          <  1:5583053/93‑1 (MQ=255)
          ccAGCGTCTGCTGCACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAg                                                                         <  2:7524755/93‑1 (MQ=255)
          ccAGCGTCTGCTGCACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAg                                                                         <  2:2605856/93‑1 (MQ=255)
          ccAGCGTCTGCTGCACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAg                                                                         <  2:6209041/93‑1 (MQ=255)
          ccAGCGTCTGCTGCACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAg                                                                         <  1:4204941/93‑1 (MQ=255)
          ccAGCGTCTGCTGCACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAg                                                                         >  1:3878798/1‑93 (MQ=255)
          ccAGCGTCTGCTGCACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGGTACGCGCCAGCAGGCTACGCATTACATgcg                                                                         >  1:3824519/1‑91 (MQ=255)
            aGCGTCTGCTGCACACACCCCCAGTAAATATCGACGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCg                                                                       <  1:8966973/93‑1 (MQ=255)
            aGCGTCTGCTGCACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCg                                                                       >  2:4059298/1‑93 (MQ=255)
            aGCGTCTGCTGCACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCCGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCg                                                                       >  1:7596710/1‑93 (MQ=255)
             gCGTCTGCTGCACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCgg                                                                      >  2:8399675/1‑93 (MQ=255)
              cGTCTGCTGCACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGga                                                                     <  2:2129348/93‑1 (MQ=255)
              cGTCTGCTGCACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGga                                                                     >  2:3608388/1‑93 (MQ=255)
              cGTCTGCCGCACACACCCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGga                                                                     <  1:308886/93‑1 (MQ=255)
               gTCTGCTGCACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGgag                                                                    <  1:4350984/93‑1 (MQ=255)
               gTCTGCTGCACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGgag                                                                    <  1:7429236/93‑1 (MQ=255)
                tCTGCTGCACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGgaga                                                                   <  1:344767/93‑1 (MQ=255)
                tCTGCTGCACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGCTACGCGCCAGCAGGCTACGACTTAAATGAGCggcac                                                                   >  1:5289928/1‑90 (MQ=255)
                  tgctgcACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGgagaga                                                                 <  1:8418175/93‑1 (MQ=255)
                  cgctgcACACCACCCCAGTAAACACCGTCGCGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGgagaga                                                                 <  1:5334229/92‑1 (MQ=255)
                   gctgcACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGAt                                                                <  2:3555747/93‑1 (MQ=255)
                   gctgcACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGAt                                                                >  1:5774641/1‑93 (MQ=255)
                   gcagcACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGAt                                                                <  1:4837588/90‑1 (MQ=255)
                     tgcACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGAtct                                                              >  2:6873218/1‑93 (MQ=255)
                     tgcACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGAtct                                                              >  2:5560055/1‑93 (MQ=255)
                       cacacaACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGAtcttc                                                            <  2:13717/93‑1 (MQ=255)
                       cacacaACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGAtcttc                                                            <  1:8027453/93‑1 (MQ=255)
                       cacacaACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGAtcttc                                                            <  1:581755/93‑1 (MQ=255)
                       cacacaACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGAtcttc                                                            <  2:7920127/93‑1 (MQ=255)
                        acacaACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGAtcttct                                                           <  1:1523412/93‑1 (MQ=255)
                         cacaACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTg                                                          >  1:4470253/1‑93 (MQ=255)
                         cacaACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGGTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTg                                                          >  2:7744005/1‑93 (MQ=255)
                          acaACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTgc                                                         <  1:7246585/93‑1 (MQ=255)
                           caACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTgca                                                        <  1:10022542/93‑1 (MQ=255)
                           caACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTgca                                                        <  1:3364411/93‑1 (MQ=255)
                             aCCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAACTGAGCGGAGAGATCTtgtgattg                                                      >  2:8478655/1‑89 (MQ=255)
                             aCCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTgcacg                                                      >  2:2320211/1‑93 (MQ=255)
                             aCCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTgcacg                                                      >  2:7282476/1‑93 (MQ=255)
                              ccccAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTgcacgc                                                     >  1:4945314/1‑93 (MQ=255)
                                ccAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTgcacgcac                                                   <  1:9376984/93‑1 (MQ=255)
                                 cAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGcacgcacg                                                  <  2:4574011/93‑1 (MQ=255)
                                  aGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGt                                                 >  1:4041689/1‑93 (MQ=255)
                                   gTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGtt                                                <  1:1407759/93‑1 (MQ=255)
                                   gTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGtt                                                <  1:8656830/93‑1 (MQ=255)
                                   gTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGtt                                                >  1:1982206/1‑93 (MQ=255)
                                   gTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGtt                                                >  2:4964876/1‑93 (MQ=255)
                                      aaTATCGTCGACGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACg                                             >  1:8691970/1‑93 (MQ=255)
                                        tatCGTCGAGGGCCGGCTCTTCCAGCGCCGGGGTACGCGCCAGCAGGCTTCGCATTAAATGAGCGAAGGGATCATTGTCATGCACGCTACgta                                           >  2:8964135/1‑91 (MQ=255)
                                        tatCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGaa                                           >  2:1783998/1‑93 (MQ=255)
                                         atCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGaaa                                          >  2:1490816/1‑93 (MQ=255)
                                          tcgtcgAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGaaaa                                         <  1:6907833/93‑1 (MQ=255)
                                          tcgtcgAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGaaaa                                         <  1:4147943/93‑1 (MQ=255)
                                           cgtcgAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAg                                        >  2:8129580/1‑93 (MQ=255)
                                            gtcgAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGc                                       <  1:2211013/93‑1 (MQ=255)
                                             tcgAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCa                                      >  2:910715/1‑93 (MQ=255)
                                              cgAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCAc                                     <  1:6254305/93‑1 (MQ=255)
                                              cgAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCAc                                     >  1:9756221/1‑93 (MQ=255)
                                              cgAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCAc                                     <  2:5172022/93‑1 (MQ=255)
                                               gAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCAcc                                    <  1:5177373/93‑1 (MQ=255)
                                               gAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCAcc                                    <  2:7444950/93‑1 (MQ=255)
                                                aGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCg                                   <  1:6789499/93‑1 (MQ=255)
                                                 gggCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGc                                  >  1:2543801/1‑93 (MQ=255)
                                                   gccgccGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGCGATCTTCTGCACGCACGTTACGAAAAGCACCGccc                                >  1:9820469/1‑93 (MQ=255)
                                                   gccgccGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGccc                                <  2:3479162/93‑1 (MQ=255)
                                                    ccgccgCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCt                               >  1:7861438/1‑93 (MQ=255)
                                                    ccgccgCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCt                               <  1:127124/93‑1 (MQ=255)
                                                      gccgcTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTc                             >  2:1220858/1‑93 (MQ=255)
                                                       ccgcTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCg                            >  1:9263401/1‑93 (MQ=255)
                                                       ccgcTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCg                            >  2:5990094/1‑93 (MQ=255)
                                                        cgcTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGa                           >  2:3310518/1‑93 (MQ=255)
                                                        cgcTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGa                           <  2:1894935/93‑1 (MQ=255)
                                                         gcTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGaa                          >  1:1841382/1‑93 (MQ=255)
                                                          cTTCCAGCGCCGGGTTACCCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGCAc                         >  1:7185911/1‑93 (MQ=255)
                                                           ttCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAACg                        >  2:7385043/1‑93 (MQ=255)
                                                            tCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAACgg                       >  2:6572731/1‑93 (MQ=255)
                                                            tCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAACgg                       <  2:2822448/93‑1 (MQ=255)
                                                                gcgcCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAACGGCCCa                   <  1:2364824/93‑1 (MQ=255)
                                                                gcgcCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAACGGCCCa                   >  1:9286509/1‑93 (MQ=255)
                                                                 cgcCGGGTTACGCACCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAACGGCCCAt                  <  1:8524664/93‑1 (MQ=255)
                                                                  gcCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAACGGCCCATc                 <  2:9834365/93‑1 (MQ=255)
                                                                   ccGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAACGGCCCATCg                >  1:284356/1‑93 (MQ=255)
                                                                    cGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAACGGCCCATCgg               <  2:6762668/93‑1 (MQ=255)
                                                                    cGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAACGGCCCATCgg               >  1:6564626/1‑93 (MQ=255)
                                                                      ggTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAACGGCCCATCgggg             >  2:5814444/1‑93 (MQ=255)
                                                                      ggTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAACGGCCCATCgggg             >  2:3106753/1‑93 (MQ=255)
                                                                        ttACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAACGGCCCATCGGGGTg           >  2:9337140/1‑93 (MQ=255)
                                                                         tACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAACGGCCCATCGGGGTGc          >  2:6487061/1‑93 (MQ=255)
                                                                            gcgcCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAACGGCCCATCGGGGTGCGaa       <  2:2953100/93‑1 (MQ=255)
                                                                               ccAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAACGGCCCATCGGGGTGCGAATTg    >  1:3212022/1‑93 (MQ=255)
                                                                                cagcagGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAACGGCCCATCGGGGTGCGCATTGc   >  1:8858052/1‑93 (MQ=255)
                                                                                cagcagGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAACGGCCCATCGGGGTGCGAATTGc   <  1:119758/93‑1 (MQ=255)
                                                                                 agcaggCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAACGGCCCATCGGGGTGCGAATTGCa  <  2:9274759/93‑1 (MQ=255)
                                                                                      ||                                                                                      
AAACCCTGCTCCAGCGTCTGCTGCACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGCGTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAACGGCCCATCGGGGTGCGAATTGCA  >  NC_000913/4026166‑4026339

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 201 total aligned reads displayed.
GATATTAAAACCCTGCTCCAGCGTCTGCTGCACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGCGTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAACGGCCCATCGGGGTGCGAATTGCATC  >  NC_000913/4026159‑4026341
                                                                                             |                                                                                         
GATATTAAAACCCTGCTCCAGCGTCTGCTGCACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGGCAGCAGG                                                                                           >  SRR4302540.4512912/1‑94 (MQ=60)
GATATTAAAACCCTGCTCCAGCGTCTGCTGCACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGG                                                                                           >  SRR4302540.9684065/1‑94 (MQ=60)
GATATTAAAACCCTGCTCCAGCGTCTGCTGCACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGG                                                                                           >  SRR4302540.2747291/1‑94 (MQ=60)
GATATTAAAACCCTGCTCCAGCGTCTGCTGCACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGG                                                                                           >  SRR4302540.4554911/1‑94 (MQ=60)
GATATTAAAACCCTGCTCCAGCGTCTGCTGCACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGGTACGCGCCAGCAGG                                                                                           >  SRR4302540.2747290/1‑94 (MQ=60)
GATATTAAAACCCTGCTCCAGCGTCTGCAGCACACAACCCCAGTAAATATCGTCGAGGGCCGCCCCTACCAGCGCCGGGTTACGCGCCAGCAGG                                                                                           >  SRR4302540.5591395/1‑94 (MQ=60)
GATAGTAAACCCCTGCTCCAGCCTCTGCTGCACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGG                                                                                           <  SRR4302540.5657168/94‑1 (MQ=60)
     TAAAACCCTGCTCCAGCGTCTGCTGCACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACG                                                                                      <  SRR4302540.8761514/94‑1 (MQ=60)
     TAAAACCCTGCTCCAGCGTCTGCTGCACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACG                                                                                      <  SRR4302540.2185315/94‑1 (MQ=60)
     TAAAACCCTGCTCCAGCGTCTGCTGCACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACG                                                                                      <  SRR4302540.605941/94‑1 (MQ=60)
      AAAACCCTGCTCCAGCGTCTGCTGCACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGC                                                                                     >  SRR4302540.5650380/1‑94 (MQ=60)
      AAAACCCTGCTCCAGCGTCTGCTGCACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGC                                                                                     >  SRR4302540.536986/1‑94 (MQ=60)
       AAACCCTGCTCCAGCGTCTGCTGCACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCA                                                                                    >  SRR4302540.7108935/1‑94 (MQ=60)
        AACCCTGCTCCAGCGTCTGCTGCACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCAT                                                                                   <  SRR4302540.5501124/94‑1 (MQ=60)
         ACCCTGCTCCAGCGTCTGCTGCACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATT                                                                                  >  SRR4302540.3889852/1‑94 (MQ=60)
          CCCTGCTCCAGCGTCTGCTGCACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTA                                                                                 <  SRR4302540.2766154/94‑1 (MQ=60)
          CCCTGCTCCAGCGTCTGCTGCACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTA                                                                                 >  SRR4302540.279048/1‑94 (MQ=60)
          CCCTGCTCCAGCGTCTGCTGCACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTA                                                                                 <  SRR4302540.2965734/94‑1 (MQ=60)
          CCCTGCTCCAGCGTCTGCTGCACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTA                                                                                 <  SRR4302540.8107559/94‑1 (MQ=60)
           CCTGCTCCAGCGTCTGCTGCACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAA                                                                                >  SRR4302540.3012/1‑94 (MQ=60)
           CCTGCTCCAGCGTCTGCTGCACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAA                                                                                >  SRR4302540.296675/1‑94 (MQ=60)
            CTGCTCCAGCGTCTGCTGCACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAA                                                                               <  SRR4302540.1682383/94‑1 (MQ=60)
             TGCTCCAGCGTCTGCTGCACACAACCCCAGTAAATATCGTCGAGGGGCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAAT                                                                              >  SRR4302540.6872167/1‑94 (MQ=60)
             TGCTCCAGCGTCTGCTGCACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAAT                                                                              <  SRR4302540.2695991/94‑1 (MQ=60)
             TGCTCCAGCGTCTGCTGCACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAAT                                                                              <  SRR4302540.5860578/94‑1 (MQ=60)
             TGCTCCAGCGTCTGCTGCACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAAT                                                                              <  SRR4302540.6160615/94‑1 (MQ=60)
               CTCCAGCGTCTGCTGCACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGA                                                                            >  SRR4302540.6524086/1‑94 (MQ=60)
               CTCCAGCGTCTGCTGCACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGA                                                                            >  SRR4302540.1230446/1‑94 (MQ=60)
               CTCCAGCGTCTGCTGCACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGA                                                                            <  SRR4302540.3126121/94‑1 (MQ=60)
               CTCCAGCGTCTGCTGCACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGGTACGCGCCAGCAGGCTACGCATTAAATGA                                                                            >  SRR4302540.93711/1‑94 (MQ=60)
                TCGAGCGTCGGCTGCACACAACCCCAGTAAATATCGTCGGGGGCCGCCGCTTCCGGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAG                                                                           <  SRR4302540.3361162/94‑1 (MQ=60)
                TCCAGCGTCTGCTGCACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAG                                                                           <  SRR4302540.5860638/94‑1 (MQ=60)
                TCCAGCGTCTGCTGCACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAG                                                                           >  SRR4302540.3920951/1‑94 (MQ=60)
                TCCAGCGTCTGCTGCACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAG                                                                           <  SRR4302540.5644507/94‑1 (MQ=60)
                TCCAGCGTCTGCTGCACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGGTACGCGCCAGCAGGCTACGCATTACATGCG                                                                           >  SRR4302540.3866136/1‑94 (MQ=60)
                 CCAGCGTCTGCTGCACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGC                                                                          <  SRR4302540.4250704/94‑1 (MQ=60)
                  CAGCGTCTGCTGCACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTAGGCGCCAGCAGGCTACGCATTAAATGAGCG                                                                         >  SRR4302540.3885194/1‑94 (MQ=60)
                  CAGCGTCTGCTGCACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCG                                                                         >  SRR4302540.8980559/1‑94 (MQ=60)
                  CAGCGTCTGCTGCACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCCGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCG                                                                         >  SRR4302540.7682055/1‑94 (MQ=60)
                   CGCGTCTGCTGCACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGG                                                                        <  SRR4302540.913436/94‑1 (MQ=60)
                   AGCGTCTGCTGCACACACCCCCAGTAAATATCGACGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGG                                                                        <  SRR4302540.9073274/94‑1 (MQ=60)
                   AGCGTCTGCTGCACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGG                                                                        <  SRR4302540.1911062/94‑1 (MQ=60)
                    GCGTCTGCTGCACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGA                                                                       <  SRR4302540.769987/94‑1 (MQ=60)
                    GCGTCTGCTGCACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGA                                                                       <  SRR4302540.8565711/94‑1 (MQ=60)
                     CGTCTGCTGCACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAG                                                                      >  SRR4302540.7011403/1‑94 (MQ=60)
                     CGTCTGCCGCACACACCCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAG                                                                      <  SRR4302540.311948/94‑1 (MQ=60)
                      GTCTGCTGCACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGA                                                                     <  SRR4302540.7511996/94‑1 (MQ=60)
                      GTCTGCTGCACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGA                                                                     >  SRR4302540.4111389/1‑94 (MQ=60)
                      GTCTGCTGCACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGA                                                                     <  SRR4302540.4398314/94‑1 (MQ=60)
                      GTCTGCTGCACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGA                                                                     >  SRR4302540.10048068/1‑94 (MQ=60)
                      GTCTGCTGCACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGA                                                                     >  SRR4302540.1501846/1‑94 (MQ=60)
                      GTCTGCTGCACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGA                                                                     >  SRR4302540.2209759/1‑94 (MQ=60)
                      GTCTGCTGCACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGA                                                                     >  SRR4302540.1387171/1‑94 (MQ=60)
                      GTCTGCTGCACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGA                                                                     >  SRR4302540.2273887/1‑94 (MQ=60)
                       TCTGCTGCACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAG                                                                    >  SRR4302540.4649960/1‑94 (MQ=60)
                       TCTGCTGCACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAG                                                                    <  SRR4302540.4225145/94‑1 (MQ=60)
                       TCTGCTGCACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAG                                                                    <  SRR4302540.348269/94‑1 (MQ=60)
                        CTGCTGCACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGA                                                                   >  SRR4302540.7588923/1‑94 (MQ=60)
                         TGCTGCACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGAT                                                                  >  SRR4302540.5838430/1‑94 (MQ=60)
                         TGCTGCACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGAT                                                                  >  SRR4302540.2186861/1‑94 (MQ=60)
                         TGCTGCACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGAT                                                                  <  SRR4302540.3604262/94‑1 (MQ=60)
                         TGCTGCACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGAT                                                                  <  SRR4302540.8515896/94‑1 (MQ=60)
                         CGCTGCACACCACCCCAGTAAACACCGTCGCGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGAT                                                                  <  SRR4302540.5392690/94‑1 (MQ=60)
                          GCAGCACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATC                                                                 <  SRR4302540.4889409/94‑1 (MQ=60)
                            TGCACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTT                                                               >  SRR4302540.3944139/1‑94 (MQ=60)
                             GCACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTC                                                              <  SRR4302540.449309/94‑1 (MQ=60)
                              CACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCT                                                             <  SRR4302540.6849985/94‑1 (MQ=60)
                              CACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCT                                                             <  SRR4302540.8120803/94‑1 (MQ=60)
                              CACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCT                                                             <  SRR4302540.588046/94‑1 (MQ=60)
                              CACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCT                                                             <  SRR4302540.4172493/94‑1 (MQ=60)
                              CACACAACCCCAGTAAATATCGACGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTAT                                                             <  SRR4302540.9041599/94‑1 (MQ=60)
                               ACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTG                                                            >  SRR4302540.6214410/1‑94 (MQ=60)
                               ACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTG                                                            >  SRR4302540.4518728/1‑94 (MQ=60)
                               ACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTG                                                            <  SRR4302540.1540273/94‑1 (MQ=60)
                                 ACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCA                                                          >  SRR4302540.1189695/1‑94 (MQ=60)
                                 ACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCA                                                          <  SRR4302540.7327435/94‑1 (MQ=60)
                                  CAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCAC                                                         <  SRR4302540.10139174/94‑1 (MQ=60)
                                  CAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCAC                                                         <  SRR4302540.3401223/94‑1 (MQ=60)
                                  CAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCAC                                                         <  SRR4302540.6762088/94‑1 (MQ=60)
                                   AACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACG                                                        <  SRR4302540.9650585/94‑1 (MQ=60)
                                    ACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGC                                                       >  SRR4302540.4980057/1‑94 (MQ=60)
                                    ACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGC                                                       >  SRR4302540.4998698/1‑94 (MQ=60)
                                     CCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCA                                                      >  SRR4302540.4977183/1‑94 (MQ=60)
                                      CCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGGGCCAGCAGGCTACGCATTAAATGAGCGGAGcaatctcctcaaaccac                                                     >  SRR4302540.9912602/1‑77 (MQ=60)
                                      CCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCCCGCAC                                                     >  SRR4302540.2362891/1‑94 (MQ=60)
                                      CCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCAC                                                     >  SRR4302540.6009694/1‑94 (MQ=60)
                                      CCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCAC                                                     >  SRR4302540.5042960/1‑94 (MQ=60)
                                      CCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCAC                                                     <  SRR4302540.9921687/94‑1 (MQ=60)
                                       CCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACG                                                    <  SRR4302540.9488609/94‑1 (MQ=60)
                                       CCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACG                                                    <  SRR4302540.5348079/94‑1 (MQ=60)
                                        CAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGT                                                   >  SRR4302540.4085257/1‑94 (MQ=60)
                                         AGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTT                                                  >  SRR4302540.2003700/1‑94 (MQ=60)
                                         AGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTT                                                  >  SRR4302540.8498827/1‑94 (MQ=60)
                                         AGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTT                                                  >  SRR4302540.8498826/1‑94 (MQ=60)
                                         AGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTT                                                  <  SRR4302540.2701868/94‑1 (MQ=60)
                                         AGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTT                                                  <  SRR4302540.3467303/94‑1 (MQ=60)
                                          GTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTA                                                 >  SRR4302540.3400792/1‑94 (MQ=60)
                                          GTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTA                                                 <  SRR4302540.8757391/94‑1 (MQ=60)
                                          GTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTA                                                 <  SRR4302540.1422916/94‑1 (MQ=60)
                                           TAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTAC                                                <  SRR4302540.8862531/94‑1 (MQ=60)
                                           TAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTAC                                                >  SRR4302540.9405475/1‑94 (MQ=60)
                                            AAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACG                                               <  SRR4302540.7428583/94‑1 (MQ=60)
                                            AAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACG                                               >  SRR4302540.3395824/1‑94 (MQ=60)
                                            AAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACG                                               >  SRR4302540.8792882/1‑94 (MQ=60)
                                            AAATATCGTCGACGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACG                                               >  SRR4302540.8792881/1‑94 (MQ=60)
                                             AATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGA                                              >  SRR4302540.4262349/1‑94 (MQ=60)
                                              acatcgtacgGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAA                                             <  SRR4302540.2004234/84‑1 (MQ=60)
                                              ATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAA                                             <  SRR4302540.8771180/94‑1 (MQ=60)
                                              ATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAA                                             >  SRR4302540.5303234/1‑94 (MQ=60)
                                                ATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAACA                                           >  SRR4302540.5655374/1‑94 (MQ=60)
                                                ATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAA                                           <  SRR4302540.3188313/94‑1 (MQ=60)
                                                 TCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAG                                          <  SRR4302540.6984672/94‑1 (MQ=60)
                                                 TCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAG                                          <  SRR4302540.9887616/94‑1 (MQ=60)
                                                 TCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAG                                          <  SRR4302540.4192850/94‑1 (MQ=60)
                                                  CGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGC                                         <  SRR4302540.6051271/94‑1 (MQ=60)
                                                   GTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCA                                        <  SRR4302540.2234895/94‑1 (MQ=60)
                                                    TCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCAC                                       >  SRR4302540.9870678/1‑94 (MQ=60)
                                                     CGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACC                                      <  SRR4302540.6323892/94‑1 (MQ=60)
                                                     CGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACC                                      <  SRR4302540.3054797/94‑1 (MQ=60)
                                                     CGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACC                                      <  SRR4302540.2047472/94‑1 (MQ=60)
                                                      GAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGTAGAGATCTTCTGCACGCACGTTACGAAAAGAACCG                                     >  SRR4302540.8642944/1‑94 (MQ=60)
                                                      GAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCG                                     >  SRR4302540.3605518/1‑94 (MQ=60)
                                                      GAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCG                                     <  SRR4302540.5233723/94‑1 (MQ=60)
                                                      GAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCG                                     >  SRR4302540.4193972/1‑94 (MQ=60)
                                                       AGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGC                                    >  SRR4302540.2571223/1‑94 (MQ=60)
                                                       AGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGC                                    <  SRR4302540.6864582/94‑1 (MQ=60)
                                                       AGGGCCGCCGCTTCCAGCGCCGGGGTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGC                                    >  SRR4302540.924176/1‑94 (MQ=60)
                                                        GGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCC                                   >  SRR4302540.1996600/1‑94 (MQ=60)
                                                         GGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCC                                  >  SRR4302540.2397393/1‑94 (MQ=60)
                                                          GCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCT                                 <  SRR4302540.6038064/94‑1 (MQ=60)
                                                          GCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCT                                 >  SRR4302540.7953291/1‑94 (MQ=60)
                                                           CCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTT                                <  SRR4302540.128464/94‑1 (MQ=60)
                                                            CGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTC                               >  SRR4302540.510385/1‑94 (MQ=60)
                                                            CGCCGCTTCCAGCGCCGGATTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGCTCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTC                               <  SRR4302540.1860481/94‑1 (MQ=60)
                                                             GCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCG                              >  SRR4302540.8945865/1‑94 (MQ=60)
                                                             GCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCG                              >  SRR4302540.9374211/1‑94 (MQ=60)
                                                              CCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGA                             >  SRR4302540.24828/1‑94 (MQ=60)
                                                               CGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAA                            >  SRR4302540.855848/1‑94 (MQ=60)
                                                               CGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAA                            <  SRR4302540.577696/94‑1 (MQ=60)
                                                               CGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAA                            <  SRR4302540.4790147/94‑1 (MQ=60)
                                                               CGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAA                            >  SRR4302540.5503255/1‑94 (MQ=60)
                                                               CGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAA                            >  SRR4302540.1861554/1‑94 (MQ=60)
                                                                GCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAAC                           <  SRR4302540.4288957/94‑1 (MQ=60)
                                                                GCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAAC                           >  SRR4302540.7266014/1‑94 (MQ=60)
                                                                GCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAAC                           >  SRR4302540.726794/1‑94 (MQ=60)
                                                                GCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAAC                           >  SRR4302540.7916060/1‑94 (MQ=60)
                                                                GCTTCCAGCGCCGGGTTACGCGCCAGCAGGATACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAAC                           <  SRR4302540.6191945/94‑1 (MQ=60)
                                                                GCTTCCAGCGCCGGGTTACCCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGCAC                           >  SRR4302540.7266016/1‑94 (MQ=60)
                                                                 CTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAACG                          <  SRR4302540.7546990/94‑1 (MQ=60)
                                                                 CTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAACG                          <  SRR4302540.8754486/94‑1 (MQ=60)
                                                                 CTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAACG                          >  SRR4302540.6357984/1‑94 (MQ=60)
                                                                 CTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAACG                          >  SRR4302540.1479008/1‑94 (MQ=60)
                                                                 CTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAACG                          >  SRR4302540.3917137/1‑94 (MQ=60)
                                                                  TTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAACGG                         >  SRR4302540.9374434/1‑94 (MQ=60)
                                                                   TCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAACGGC                        >  SRR4302540.934514/1‑94 (MQ=60)
                                                                    CCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAACGGCC                       >  SRR4302540.7498565/1‑94 (MQ=60)
                                                                    CCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAACGGCC                       <  SRR4302540.1083782/94‑1 (MQ=60)
                                                                    CCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAACGGCC                       <  SRR4302540.8685941/94‑1 (MQ=60)
                                                                     CAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAACGGCCC                      >  SRR4302540.4121738/1‑94 (MQ=60)
                                                                      CGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAACGGCCCA                     <  SRR4302540.2991357/94‑1 (MQ=60)
                                                                      AGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAACGGCCCA                     <  SRR4302540.2655842/94‑1 (MQ=60)
                                                                      AGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAACGGCCCA                     >  SRR4302540.9397482/1‑94 (MQ=60)
                                                                       GCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAACGGCCCAT                    >  SRR4302540.4027891/1‑94 (MQ=60)
                                                                       GCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAACGGCCCAT                    <  SRR4302540.9405193/94‑1 (MQ=60)
                                                                       GCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAACGGCCCAT                    <  SRR4302540.2390289/94‑1 (MQ=60)
                                                                       GCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAACGGCCCAT                    <  SRR4302540.6941741/94‑1 (MQ=60)
                                                                        CGCCGGGTTACGCACCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAACGGCCCATC                   <  SRR4302540.8623721/94‑1 (MQ=60)
                                                                         GCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAACGGCCCATCG                  <  SRR4302540.7985159/94‑1 (MQ=60)
                                                                         GCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAACGGCCCATCG                  >  SRR4302540.287156/1‑94 (MQ=60)
                                                                          CCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAACGGCCCATCGG                 >  SRR4302540.10115866/1‑94 (MQ=60)
                                                                          CCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAACGGCCCATCGG                 >  SRR4302540.6637379/1‑94 (MQ=60)
                                                                           CGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAACGGCCCATCGGG                <  SRR4302540.3401822/94‑1 (MQ=60)
                                                                           CGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAACGGCCCATCGGG                <  SRR4302540.5359716/94‑1 (MQ=60)
                                                                            GGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAACGGCCCATCGGGG               >  SRR4302540.251343/1‑94 (MQ=60)
                                                                            GGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAACGGCCCATCGGGG               >  SRR4302540.4497676/1‑94 (MQ=60)
                                                                            GGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAACGGCCCATCGGGG               >  SRR4302540.5116294/1‑94 (MQ=60)
                                                                             GGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAACGGCCCATCGGGGT              <  SRR4302540.7343457/94‑1 (MQ=60)
                                                                             GGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAACGGCCCATCGGGGT              >  SRR4302540.5343834/1‑94 (MQ=60)
                                                                              GTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGATAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAACGGCCCATCGGGGTG             >  SRR4302540.8479643/1‑94 (MQ=60)
                                                                              GTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAACGGCCCATCGGGGTG             >  SRR4302540.1306440/1‑94 (MQ=60)
                                                                               TTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAACGGCCCATCGGGGTGC            <  SRR4302540.6681585/94‑1 (MQ=60)
                                                                                 ACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAACGGCCCATCGGGGTGCGA          >  SRR4302540.4770923/1‑94 (MQ=60)
                                                                                   GCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAACGGCCCATCGGGGTGCGAAT        <  SRR4302540.7858386/94‑1 (MQ=60)
                                                                                   GCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAACGGCCCATCGGGGTGCGAAT        >  SRR4302540.8768517/1‑94 (MQ=60)
                                                                                    CGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAACGGCCCATCGGGGTGCGAATT       <  SRR4302540.7313816/94‑1 (MQ=60)
                                                                                    CGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAACGGCCCATCGGGGTGCGAATT       <  SRR4302540.2649842/94‑1 (MQ=60)
                                                                                    CGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAACGGCCCATCGGGGTGCGAATT       >  SRR4302540.1795898/1‑94 (MQ=60)
                                                                                     GCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAACGGCCCATCGGGGTGCGAATTG      >  SRR4302540.3246892/1‑94 (MQ=60)
                                                                                     GCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAACGGCCCATCGGGGTGCGAATTG      >  SRR4302540.287371/1‑94 (MQ=60)
                                                                                     GCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAACGGCCCATCGGGGTGCGAATTG      >  SRR4302540.9730435/1‑94 (MQ=60)
                                                                                     GCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAACGGCCCATCGGGGTGCGAATTG      >  SRR4302540.9730434/1‑94 (MQ=60)
                                                                                      CCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAACGGCCCATCGGGGTGCGCATTGC     >  SRR4302540.8960547/1‑94 (MQ=60)
                                                                                      CCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAACGGCCCATCGGGGTGCGAATTGC     >  SRR4302540.1353461/1‑94 (MQ=60)
                                                                                       CAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAACGGCCCATCGGGGTGCGAATTGCA    <  SRR4302540.121046/94‑1 (MQ=60)
                                                                                       CAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAACGGCCCATCGGGGTGCGAATTGCA    <  SRR4302540.121047/94‑1 (MQ=60)
                                                                                        AGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAACGGCCCATCGGGGTGCGAATTGCAT   >  SRR4302540.7538746/1‑94 (MQ=60)
                                                                                        AGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAACGGCCCATCGGGGTGCGAATTGCAT   >  SRR4302540.5724784/1‑94 (MQ=60)
                                                                                         GCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAACGGCCCATCGGGGTGCGAATTGCATC  <  SRR4302540.3141904/94‑1 (MQ=60)
                                                                                         GCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAACGGCCCATCGGGGTGCGAATTGCATC  <  SRR4302540.5843131/94‑1 (MQ=60)
                                                                                         GCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAACGGCCCATCGGGGTGCGAATTGCATC  >  SRR4302540.5824547/1‑94 (MQ=60)
                                                                                             |                                                                                         
GATATTAAAACCCTGCTCCAGCGTCTGCTGCACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGCGTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAACGGCCCATCGGGGTGCGAATTGCATC  >  NC_000913/4026159‑4026341

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑