Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1548 369.5 20095359 96.9% 19472402 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,057,759 +C coding (1773/1776 nt) yihK → putative GTP‑binding factor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,057,7581.C99.8% 1865.6 / ‑4.6 563coding (1772/1776 nt)yihKputative GTP‑binding factor
Reads supporting (aligned to +/- strand):  ref base . (0/1);  new base C (327/235);  total (327/236)
Fisher's exact test for biased strand distribution p-value = 4.19e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

Only 100 of 613 total aligned reads displayed.

TATCCGCATGACTCTGGAACAAGCTCTGGAGTTCATCGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCA‑CTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATG  >  NC_000913/4057666‑4057845
                                                                                             |                                                                                       
tATCCGCATGACTCTGGAACAAGCTCTGGAGTTCATCGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCa                                                                                          >  1:6094541/1‑93 (MQ=255)
tATCCGCATGACTCTGGAACAAGCTCTGGAGTTCATCGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCa                                                                                          >  2:6257572/1‑93 (MQ=255)
 aTCCGCATGACTCTGGAACAAGCTCTGGAGTTCATCGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCA‑c                                                                                        >  1:7055755/1‑93 (MQ=255)
  tCCGCATGACTCTGGAACAAGCTCTGGAGTTCATCGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCA‑cc                                                                                       >  2:3465927/1‑92 (MQ=255)
  tCCGCATGACTCTGGAACAAGCTCTGGAGTTCATCGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCA‑cc                                                                                       >  1:2759351/1‑92 (MQ=255)
    cGCATGACTCTGGAACAAGCTCTGGAGTTCATCGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCA‑cctg                                                                                     >  1:8541417/1‑90 (MQ=255)
    cGCATGACTCTGGAACAAGCTCTGGAGTTCATCGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCA‑cctg                                                                                     >  1:1198773/1‑90 (MQ=255)
     gCATGACTCTGGAACAAGCTCTGGAGTTCATCGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCA‑cctga                                                                                    >  1:7178492/1‑89 (MQ=255)
     gCATGACTCTGGAACAAGCTCTGGAGTTCATCGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCA‑cctga                                                                                    >  2:6947833/1‑89 (MQ=255)
       aTGACTCTGGAACAAGCTCTGGAGTTCATCGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACg                                                                                   >  1:150744/1‑93 (MQ=255)
        tGACTCTGGAACAAGCTCTGGAGTTCATCGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACgg                                                                                  <  1:6562386/93‑1 (MQ=255)
         gACTCTGGAACAAGCTCTGGAGTTCATCGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGa                                                                                 >  2:8305153/1‑93 (MQ=255)
           ctctGGAACAAGCTCTGGAGTTCATCGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGaaa                                                                               <  2:3978412/93‑1 (MQ=255)
           ctctGGAACAAGCTCTGGAGTTCATCGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGaaa                                                                               <  1:4660310/93‑1 (MQ=255)
            tctGGAACAAGCTCTGGAGTTCATCGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGaaaa                                                                              >  2:5983812/1‑93 (MQ=255)
            tctGGAACAAGCTCTGGAGTTCATCGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGaaaa                                                                              <  1:1341678/93‑1 (MQ=255)
             ctGGAACAAGCTCTGGAGTTCATCGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAAc                                                                             <  2:8074591/93‑1 (MQ=255)
             ctGGAACAAGCTCTGGAGTTCATCGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAAc                                                                             <  1:2925654/93‑1 (MQ=255)
               ggAACAAGCTCTGGAGTTCATCGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGa                                                                           >  1:9717418/1‑93 (MQ=255)
                gAACAAGCTCTGGAGTTCATCGATGATGACGAACTGGTAGAAGTGACTCCGTCTTCTATCCGTATTTGTAAACGTCACCTGACGGAAAACGAt                                                                          >  2:8988629/1‑93 (MQ=255)
                gAACAAGCTCTGGAGTTCATCGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGAt                                                                          <  1:8131288/93‑1 (MQ=255)
                 aaCAAGCTCTGGAGTTCATCGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGAtc                                                                         <  1:39433/93‑1 (MQ=255)
                 aaCAAGCTCTGGAGTTCATCGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAAAGAtg                                                                         >  2:1859639/1‑92 (MQ=255)
                  aCAAGCTCTGGAGTTCATCGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGAtcg                                                                        >  1:5539550/1‑93 (MQ=255)
                  aCAAGCTCTGGAGTTCATCGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGAtcg                                                                        >  1:9977724/1‑93 (MQ=255)
                         ttGGAGTTCATCGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTAGCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTcgccgc                                                                 <  2:1973243/92‑1 (MQ=255)
                         ctGGAGTTCATCGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTcgccgc                                                                 >  1:791150/1‑93 (MQ=255)
                          tGGAGTTCATCGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCcgcg                                                                >  1:2203923/1‑93 (MQ=255)
                           ggAGTTCATCGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCgcgc                                                               >  2:9569777/1‑93 (MQ=255)
                             aGTTCATCGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCa                                                             >  1:7624478/1‑93 (MQ=255)
                              gTTCATCGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCaa                                                            <  2:5350947/93‑1 (MQ=255)
                              gTTCATCGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCaa                                                            >  1:6877500/1‑93 (MQ=255)
                               ttCATCGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAAc                                                           <  1:8299738/93‑1 (MQ=255)
                                 cATCGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACcg                                                         >  2:4292081/1‑93 (MQ=255)
                                   tCGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACcgcg                                                       >  2:4446169/1‑93 (MQ=255)
                                     gatgatGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGGCGCGCCAACCGCGCa                                                     >  1:9470482/1‑93 (MQ=255)
                                     gatgatGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCa                                                     <  1:474861/93‑1 (MQ=255)
                                      atgatgACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCAc                                                    >  2:2905270/1‑93 (MQ=255)
                                      atgatgACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCAc                                                    <  1:263767/93‑1 (MQ=255)
                                         atgaCGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGa                                                 >  1:4160866/1‑93 (MQ=255)
                                          tgaCGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGaa                                                >  2:3063450/1‑93 (MQ=255)
                                          tgaCGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGaa                                                >  1:8443644/1‑93 (MQ=255)
                                           gaCGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGaaa                                               <  1:1736225/93‑1 (MQ=255)
                                            aCGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAg                                              <  2:3299959/93‑1 (MQ=255)
                                            aCGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAg                                              >  2:574007/1‑93 (MQ=255)
                                             cGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGa                                             <  2:2255006/93‑1 (MQ=255)
                                               aaCTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACg                                           >  1:6405671/1‑93 (MQ=255)
                                                acgggTAGAAGTGACTACCACCTCTATCCGTATTCGTAAACGTCACCTTACGGAAAACGATCGTCCCCGCGCCAACCGCGCACCGAAAGACGa                                          <  2:5866936/90‑1 (MQ=255)
                                                aCTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGa                                          >  1:1986087/1‑93 (MQ=255)
                                                  tGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGAtt                                        >  2:6740632/1‑93 (MQ=255)
                                                    gTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTaa                                      >  2:9237884/1‑93 (MQ=255)
                                                     tAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAAt                                     >  2:7743248/1‑93 (MQ=255)
                                                      aGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAAtt                                    >  2:1693403/1‑93 (MQ=255)
                                                       gAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAAttt                                   >  1:9366473/1‑93 (MQ=255)
                                                        aaGTGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTc                                  >  1:1120471/1‑93 (MQ=255)
                                                        aaGTGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAACACGAGTAATTTc                                  >  1:2451009/1‑93 (MQ=255)
                                                         aGTGACTCCTACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAAtttag                                 >  2:2641525/1‑91 (MQ=255)
                                                         aGTGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCg                                 <  2:7565362/93‑1 (MQ=255)
                                                             aCTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGtttt                             <  1:5002132/93‑1 (MQ=255)
                                                              cTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGttttt                            >  1:5287862/1‑93 (MQ=255)
                                                               tCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGtttttt                           <  2:413883/93‑1 (MQ=255)
                                                                ccGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTa                          <  2:8739573/93‑1 (MQ=255)
                                                                  gACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGt                        <  2:1214232/93‑1 (MQ=255)
                                                                   aCCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTc                       >  1:7403629/1‑93 (MQ=255)
                                                                   aCCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTc                       <  2:5059769/93‑1 (MQ=255)
                                                                   aCCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTc                       <  2:4803788/93‑1 (MQ=255)
                                                                    ccTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCa                      >  1:5720147/1‑93 (MQ=255)
                                                                     ctctATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCAt                     >  2:9375922/1‑93 (MQ=255)
                                                                     ctctATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCAt                     >  2:5616999/1‑93 (MQ=255)
                                                                      tctATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATa                    >  2:8498678/1‑93 (MQ=255)
                                                                      tctATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATa                    >  2:5159088/1‑93 (MQ=255)
                                                                      tctATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATa                    >  2:1564801/1‑93 (MQ=255)
                                                                      tctATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATa                    >  2:3837063/1‑93 (MQ=255)
                                                                       ctATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATaa                   <  1:669151/93‑1 (MQ=255)
                                                                       ctATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATaa                   <  2:540756/93‑1 (MQ=255)
                                                                        tATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATaaa                  >  2:1051755/1‑93 (MQ=255)
                                                                         aTCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATaaaa                 >  1:379679/1‑93 (MQ=255)
                                                                          tCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATaaaaa                >  1:5104348/1‑93 (MQ=255)
                                                                          tCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATaaaaa                >  1:4917411/1‑93 (MQ=255)
                                                                           ccGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATaaaaaa               >  2:3721579/1‑93 (MQ=255)
                                                                           ccGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATaaaaaa               >  1:4259416/1‑93 (MQ=255)
                                                                           ccGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATaaaaaa               <  2:7328698/93‑1 (MQ=255)
                                                                            cGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAAc              >  2:6486406/1‑93 (MQ=255)
                                                                            cGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAAc              <  1:8001408/93‑1 (MQ=255)
                                                                              tATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCt            >  1:9087911/1‑93 (MQ=255)
                                                                              tATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCt            <  1:6256557/93‑1 (MQ=255)
                                                                               aTTCGTAAACGTCACCTGACGTAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTg           <  2:2127945/93‑1 (MQ=255)
                                                                               aTTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTg           >  1:3662156/1‑93 (MQ=255)
                                                                               aTTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTg           >  2:3571802/1‑93 (MQ=255)
                                                                                 tCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGcc         <  2:4707469/93‑1 (MQ=255)
                                                                                   gTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAg       >  1:4451675/1‑93 (MQ=255)
                                                                                   gTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAg       <  1:5208515/93‑1 (MQ=255)
                                                                                   gTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAg       >  2:1392904/1‑93 (MQ=255)
                                                                                    tAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGc      >  1:3430589/1‑93 (MQ=255)
                                                                                    tAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGc      >  1:4586363/1‑93 (MQ=255)
                                                                                     aaaCGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCg     >  1:10005899/1‑93 (MQ=255)
                                                                                      aaCGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGa    <  1:9286172/93‑1 (MQ=255)
                                                                                      aaCGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGa    <  1:3069324/93‑1 (MQ=255)
                                                                                      aaCGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGa    <  1:6027684/93‑1 (MQ=255)
                                                                                        cGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATg  >  2:7199473/1‑93 (MQ=255)
                                                                                             |                                                                                       
TATCCGCATGACTCTGGAACAAGCTCTGGAGTTCATCGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCA‑CTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATG  >  NC_000913/4057666‑4057845

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 345 total aligned reads displayed.
TATCCGCATGACTCTGGAACAAGCTCTGGAGTTCATCGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCA‑CTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGTT  >  NC_000913/4057666‑4057852
                                                                                              |                                                                                             
TATCCGCATGACTCTGGAACAAGCTCTGGAGTTCATCGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCA‑C                                                                                               >  SRR4302540.7134220/1‑94 (MQ=60)
TATCCGCATGACTCTGGAACAAGCTCTGGAGTTCATCGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCA‑C                                                                                               >  SRR4302540.8618363/1‑94 (MQ=60)
 ATCCGCATGACTCTGGAACAAGCTCTGGAGTTCATCGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCA‑CC                                                                                              >  SRR4302540.2789161/1‑94 (MQ=60)
 ATCCGCATGACTCTGGAACAAGCTCTGGAGTTCATCGATGATGACGAACTGGGAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCA‑CC                                                                                              >  SRR4302540.2789160/1‑94 (MQ=60)
   CCGCATGACTCTGGAACAAGCTCTGGAGTTCATCGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCA‑Cctg                                                                                            >  SRR4302540.1211104/1‑91 (MQ=60)
    CGCATGACTCTGGAACAAGCTCTGGAGTTCATCGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGA                                                                                            >  SRR4302540.602645/1‑94 (MQ=60)
    CGCATGACTCTGGAACAAGCTCTGGAGTTCATCGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGA                                                                                            >  SRR4302540.7258516/1‑94 (MQ=60)
    CGCATGACTCTGGAACAAGCTCTGGAGTTCATCGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGA                                                                                            >  SRR4302540.4501466/1‑94 (MQ=60)
     GCATGACTCTGGAACAAGCTCTGGAGTTCATCGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGAC                                                                                           >  SRR4302540.4787829/1‑94 (MQ=60)
     GCATGACTCTGGAACAAGCTCTGGAGTTCATCGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGAC                                                                                           <  SRR4302540.10100163/94‑1 (MQ=60)
      CATGACTCTGGAACAAGCTCTGGAGTTCATCGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACG                                                                                          <  SRR4302540.9058254/94‑1 (MQ=60)
       ATGACTCTGGAACAAGCTCTGGAGTTCATCGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGG                                                                                         >  SRR4302540.9820959/1‑94 (MQ=60)
       ATGACTCTGGAACAAGCTCTGGAGTTCATCGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGG                                                                                         <  SRR4302540.10051164/94‑1 (MQ=60)
       ATGACTCTGGAACAAGCTCTGGAGTTCATCGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGG                                                                                         >  SRR4302540.137949/1‑94 (MQ=60)
        TGACTCTGGAACAAGCTCTGGAGTTCATCGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGA                                                                                        <  SRR4302540.6635124/94‑1 (MQ=60)
         GACTCTGGAACAAGCTCTGGAGTTCATCGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAA                                                                                       <  SRR4302540.724370/94‑1 (MQ=60)
          ACTCTGGAACAAGCTCTGGAGTTCATCGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAA                                                                                      >  SRR4302540.2668786/1‑94 (MQ=60)
          ACTCTGGAACAAGCTCTGGAGTTCATCGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAA                                                                                      <  SRR4302540.1293972/94‑1 (MQ=60)
           CTCTGGAACAAGCTCTGGAGTTCATCGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAA                                                                                     >  SRR4302540.167597/1‑94 (MQ=60)
           CTCTGGAACAAGCTCTGGAGTTCATCGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAA                                                                                     <  SRR4302540.4710194/94‑1 (MQ=60)
           CTCTGGAACAAGCTCTGGAGTTCATCGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAA                                                                                     >  SRR4302540.4043639/1‑94 (MQ=60)
           CTCTGGAACAAGCTCTGGAGTTCATCGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAA                                                                                     <  SRR4302540.2980216/94‑1 (MQ=60)
            TCTGGAACAAGCTCTGGAGTTCATCGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAAC                                                                                    <  SRR4302540.2285142/94‑1 (MQ=60)
               GGAACAAGCTCTGGAGTTCATCGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGAT                                                                                 <  SRR4302540.1978206/94‑1 (MQ=60)
                GAACAAGCTCTGGAGTTCATCGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATC                                                                                >  SRR4302540.3731426/1‑94 (MQ=60)
                GAACAAGCTCTGGAGTTCATCGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATC                                                                                <  SRR4302540.6453307/94‑1 (MQ=60)
                GAACAAGCTCTGGAGTTCATCGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATC                                                                                <  SRR4302540.8225671/94‑1 (MQ=60)
                 AACAAGCTCTGGAGTTCATCGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCG                                                                               <  SRR4302540.3552632/94‑1 (MQ=60)
                 AACAAGCTCTGGAGTTCATCGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCG                                                                               <  SRR4302540.39839/94‑1 (MQ=60)
                 AACAAGCTCTGGAGTTCATCGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCG                                                                               <  SRR4302540.6221560/94‑1 (MQ=60)
                 AACAAGCTCTGGAGTTCATCGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCG                                                                               >  SRR4302540.5600566/1‑94 (MQ=60)
                  ACAAGCTCTGGAGTTCATCGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGT                                                                              <  SRR4302540.9520385/94‑1 (MQ=60)
                   CAAGCTCTGGAGTTCATCGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTC                                                                             <  SRR4302540.8503262/94‑1 (MQ=60)
                    AAGCTCTGGAGTTCATCGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCG                                                                            >  SRR4302540.3059091/1‑94 (MQ=60)
                    AAGCTCTGGAGTTCATCGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCG                                                                            >  SRR4302540.3089625/1‑94 (MQ=60)
                    AAGCTCTGGAGTTCATCGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCG                                                                            <  SRR4302540.8953563/94‑1 (MQ=60)
                    AAGCTCTGGAGTTCATCGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCG                                                                            <  SRR4302540.8146725/94‑1 (MQ=60)
                     AGCTCTGGAGTTCATCGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGC                                                                           >  SRR4302540.787008/1‑94 (MQ=60)
                      GCTGTGGAGTTCATCGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCC                                                                          <  SRR4302540.1225165/94‑1 (MQ=60)
                      GCTCTGGAGTTCATCGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCC                                                                          <  SRR4302540.6192972/94‑1 (MQ=60)
                      GCTCGGGAGTTCATCGATGATGACGAACTGGTAAAAGTGACTCAGAGCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCC                                                                          <  SRR4302540.9844577/94‑1 (MQ=60)
                       CTCTGGAGTTCATCGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCG                                                                         >  SRR4302540.7302252/1‑94 (MQ=60)
                       CTCTGGAGTTCATCGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCG                                                                         >  SRR4302540.8469513/1‑94 (MQ=60)
                       CTCTGGAGTTCATCGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCG                                                                         >  SRR4302540.7301981/1‑94 (MQ=60)
                       CTCTGGAGTTCATCGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCG                                                                         >  SRR4302540.6380142/1‑94 (MQ=60)
                       CTCTGGAGTTCATCGATGATGACGAACGGGTAGAAGGGACTCCGACCTCTATCCGCATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCG                                                                         >  SRR4302540.3614075/1‑94 (MQ=60)
                        TCTGGAGTTCATCGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGC                                                                        >  SRR4302540.7068893/1‑94 (MQ=60)
                        TCTGGAGTTCATCGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGC                                                                        >  SRR4302540.799554/1‑94 (MQ=60)
                           GGAGTTCATCGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCC                                                                     >  SRR4302540.469321/1‑94 (MQ=60)
                            GAGTTCATCGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCA                                                                    >  SRR4302540.7710218/1‑94 (MQ=60)
                            GAGTTCATCGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCA                                                                    >  SRR4302540.9427401/1‑94 (MQ=60)
                            GAGTTCATCGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCA                                                                    <  SRR4302540.9573172/94‑1 (MQ=60)
                            GAGTTCATCGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCA                                                                    >  SRR4302540.5012277/1‑94 (MQ=60)
                            GAGTTCATCGATGATGACGAACTGGTAGAAGTCACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCA                                                                    <  SRR4302540.9573171/94‑1 (MQ=60)
                              GTTCATCGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAAC                                                                  >  SRR4302540.4757784/1‑94 (MQ=60)
                              GTTCATCGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAAC                                                                  >  SRR4302540.5339049/1‑94 (MQ=60)
                               TTCATCGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACC                                                                 <  SRR4302540.1552498/94‑1 (MQ=60)
                                TCATCGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCG                                                                >  SRR4302540.6605286/1‑94 (MQ=60)
                                TCATCGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCG                                                                >  SRR4302540.6732704/1‑94 (MQ=60)
                                TCATCGATGATGACGAACTGGGAGAAGTGAGTCCGAGCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATGGTGGGCGCGCCAACGG                                                                >  SRR4302540.2861911/1‑94 (MQ=60)
                                 CATCGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGC                                                               <  SRR4302540.4753724/94‑1 (MQ=60)
                                  ATCGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGGGCCAACCGCG                                                              >  SRR4302540.9469663/1‑94 (MQ=60)
                                  ATCGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCG                                                              >  SRR4302540.2707216/1‑94 (MQ=60)
                                  ATCGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCC                                                              >  SRR4302540.5749771/1‑94 (MQ=60)
                                   TCGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGC                                                             >  SRR4302540.6488398/1‑94 (MQ=60)
                                   TCGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGC                                                             <  SRR4302540.5136607/94‑1 (MQ=60)
                                    CGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCA                                                            >  SRR4302540.5177354/1‑94 (MQ=60)
                                    CGATGATGACGAACGGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCA                                                            <  SRR4302540.2587277/94‑1 (MQ=60)
                                     GATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCAC                                                           <  SRR4302540.479946/94‑1 (MQ=60)
                                     GATGATGACGAACTGGTAGAAGCGAATCAGACCTCTATCCGTATTCGTAAACATCACCTGACGGAAAACGCTCGTCGCCGCCCCAACCGCGCAC                                                           >  SRR4302540.8066174/1‑94 (MQ=60)
                                       TGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAAGGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCG                                                         >  SRR4302540.1820339/1‑94 (MQ=60)
                                       TGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCG                                                         <  SRR4302540.4150400/94‑1 (MQ=60)
                                        GATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGA                                                        >  SRR4302540.1176455/1‑94 (MQ=60)
                                         ATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAA                                                       >  SRR4302540.8541662/1‑94 (MQ=60)
                                         ATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAA                                                       >  SRR4302540.9947076/1‑94 (MQ=60)
                                         ATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAA                                                       <  SRR4302540.5685382/94‑1 (MQ=60)
                                         ATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAA                                                       <  SRR4302540.1519118/94‑1 (MQ=60)
                                         ATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAA                                                       >  SRR4302540.2092917/1‑94 (MQ=60)
                                             CGAACTGGTAGAAGTGCCTCCGACCTCTATCCGTATTCGTAAACGGCACCTGCCGGAAATCGATCGTCGCCGCGCCAACCGCGCACCGAAAGAC                                                   <  SRR4302540.1708203/94‑1 (MQ=60)
                                             CGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGAC                                                   <  SRR4302540.8203711/94‑1 (MQ=60)
                                             CGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGAC                                                   <  SRR4302540.5175353/94‑1 (MQ=60)
                                              GAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACG                                                  <  SRR4302540.8777195/94‑1 (MQ=60)
                                              GAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACG                                                  <  SRR4302540.133070/94‑1 (MQ=60)
                                              GAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACG                                                  >  SRR4302540.4695787/1‑94 (MQ=60)
                                              GAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACG                                                  <  SRR4302540.8670149/94‑1 (MQ=60)
                                               AACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGA                                                 >  SRR4302540.8578400/1‑94 (MQ=60)
                                               AACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGA                                                 >  SRR4302540.2007619/1‑94 (MQ=60)
                                               AACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGA                                                 >  SRR4302540.7356548/1‑94 (MQ=60)
                                               AACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGA                                                 >  SRR4302540.4285079/1‑94 (MQ=60)
                                                ACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGAT                                                >  SRR4302540.9813958/1‑94 (MQ=60)
                                                ACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGAT                                                <  SRR4302540.4355823/94‑1 (MQ=60)
                                                 CTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATT                                               >  SRR4302540.6706053/1‑94 (MQ=60)
                                                 CTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATT                                               <  SRR4302540.2956151/94‑1 (MQ=60)
                                                 CTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATT                                               >  SRR4302540.798060/1‑94 (MQ=60)
                                                  TGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTA                                              <  SRR4302540.2337650/94‑1 (MQ=60)
                                                   GGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGAacattat                                             >  SRR4302540.2324670/1‑87 (MQ=60)
                                                   GGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAA                                             <  SRR4302540.3945540/94‑1 (MQ=60)
                                                   GGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAA                                             >  SRR4302540.3909415/1‑94 (MQ=60)
                                                     TAGAAGTGACCCCGCCCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATT                                           <  SRR4302540.7594791/94‑1 (MQ=60)
                                                      AGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTT                                          <  SRR4302540.6105461/94‑1 (MQ=60)
                                                      AGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTT                                          >  SRR4302540.9478002/1‑94 (MQ=60)
                                                      AGAAGTCACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTT                                          <  SRR4302540.6105462/94‑1 (MQ=60)
                                                       GAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTC                                         <  SRR4302540.1049988/94‑1 (MQ=60)
                                                       GAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTC                                         >  SRR4302540.1132099/1‑94 (MQ=60)
                                                       GAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTC                                         >  SRR4302540.2477402/1‑94 (MQ=60)
                                                        AAGTGCCTCCGACCTCTATCCGTATTCGTAACCGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCG                                        <  SRR4302540.2063111/94‑1 (MQ=60)
                                                        AAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCG                                        <  SRR4302540.9389336/94‑1 (MQ=60)
                                                        AAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCG                                        >  SRR4302540.9671104/1‑94 (MQ=60)
                                                        AAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCG                                        <  SRR4302540.677719/94‑1 (MQ=60)
                                                         AGTGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTCATTTCGT                                       >  SRR4302540.7675418/1‑94 (MQ=60)
                                                          GTGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTT                                      >  SRR4302540.3391728/1‑94 (MQ=60)
                                                          GTGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTT                                      <  SRR4302540.8384151/94‑1 (MQ=60)
                                                          GTGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTT                                      <  SRR4302540.6601424/94‑1 (MQ=60)
                                                          GTGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTT                                      <  SRR4302540.9255361/94‑1 (MQ=60)
                                                           TGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTT                                     <  SRR4302540.500212/94‑1 (MQ=60)
                                                           TGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTT                                     <  SRR4302540.8118637/94‑1 (MQ=60)
                                                           TGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTT                                     <  SRR4302540.4159235/94‑1 (MQ=60)
                                                            GACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTT                                    >  SRR4302540.1149052/1‑94 (MQ=60)
                                                            GACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTT                                    >  SRR4302540.5257450/1‑94 (MQ=60)
                                                             ACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTT                                   <  SRR4302540.6798826/94‑1 (MQ=60)
                                                             ACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTT                                   >  SRR4302540.5345654/1‑94 (MQ=60)
                                                             ACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTT                                   <  SRR4302540.5056301/94‑1 (MQ=60)
                                                               TCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTA                                 >  SRR4302540.1093429/1‑94 (MQ=60)
                                                                CCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAG                                <  SRR4302540.8443104/94‑1 (MQ=60)
                                                                CCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAG                                <  SRR4302540.8330447/94‑1 (MQ=60)
                                                                CCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAG                                <  SRR4302540.4461227/94‑1 (MQ=60)
                                                                 CGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGT                               >  SRR4302540.5923304/1‑94 (MQ=60)
                                                                  GACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTC                              >  SRR4302540.3506555/1‑94 (MQ=60)
                                                                  GACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTC                              <  SRR4302540.2618893/94‑1 (MQ=60)
                                                                  GACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTC                              >  SRR4302540.7486110/1‑94 (MQ=60)
                                                                  GACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTC                              >  SRR4302540.9379145/1‑94 (MQ=60)
                                                                   ACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCA                             <  SRR4302540.6656607/94‑1 (MQ=60)
                                                                   ACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCA                             >  SRR4302540.7164591/1‑94 (MQ=60)
                                                                    CCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCAT                            >  SRR4302540.4209571/1‑94 (MQ=60)
                                                                    CCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCAT                            >  SRR4302540.8150648/1‑94 (MQ=60)
                                                                     CTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATA                           >  SRR4302540.4918554/1‑94 (MQ=60)
                                                                     CTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATA                           >  SRR4302540.8603117/1‑94 (MQ=60)
                                                                     CTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATA                           >  SRR4302540.4260188/1‑94 (MQ=60)
                                                                     CTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATA                           >  SRR4302540.4564078/1‑94 (MQ=60)
                                                                     CTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATA                           >  SRR4302540.5885536/1‑94 (MQ=60)
                                                                     CTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATA                           >  SRR4302540.4918553/1‑94 (MQ=60)
                                                                      TCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAA                          <  SRR4302540.2924907/94‑1 (MQ=60)
                                                                      TCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAA                          >  SRR4302540.6416129/1‑94 (MQ=60)
                                                                      TCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAA                          >  SRR4302540.7164116/1‑94 (MQ=60)
                                                                      TCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAA                          <  SRR4302540.9403379/94‑1 (MQ=60)
                                                                       CTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAA                         <  SRR4302540.4468160/94‑1 (MQ=60)
                                                                       CTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAA                         >  SRR4302540.9130995/1‑94 (MQ=60)
                                                                        TATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAA                        >  SRR4302540.5053847/1‑94 (MQ=60)
                                                                        TATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAA                        >  SRR4302540.383607/1‑94 (MQ=60)
                                                                        TATCCGCATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAATCGCGCACCGAAAGACGATTCATTTCGTTTTTTAGTCATAAAA                        >  SRR4302540.9289899/1‑94 (MQ=60)
                                                                         acgcattttcgcaaacgtcccCTGACGGAAAACGAACGTCGCCGCGCCAGCCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAA                       <  SRR4302540.4821160/73‑1 (MQ=60)
                                                                         ATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAA                       >  SRR4302540.4676872/1‑94 (MQ=60)
                                                                         ATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAA                       >  SRR4302540.4970373/1‑94 (MQ=60)
                                                                         ATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAA                       >  SRR4302540.5159605/1‑94 (MQ=60)
                                                                         ATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAA                       >  SRR4302540.2513030/1‑94 (MQ=60)
                                                                          TCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAA                      <  SRR4302540.7847478/94‑1 (MQ=60)
                                                                          TCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAA                      >  SRR4302540.4305749/1‑94 (MQ=60)
                                                                           CCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGACAGACGATTAATTTCGTTTTTTAGTCATAAAAAAC                     >  SRR4302540.1401508/1‑94 (MQ=60)
                                                                           CCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAAC                     >  SRR4302540.7399988/1‑94 (MQ=60)
                                                                            CGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACC                    >  SRR4302540.5582627/1‑94 (MQ=60)
                                                                            CGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACC                    <  SRR4302540.8094533/94‑1 (MQ=60)
                                                                             GTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCT                   <  SRR4302540.394488/94‑1 (MQ=60)
                                                                             GTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCT                   >  SRR4302540.9197086/1‑94 (MQ=60)
                                                                             GTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACAGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCT                   >  SRR4302540.7115144/1‑94 (MQ=60)
                                                                             GTATTCGTAAACGTCACCTGACGGAAAACGAACGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCT                   >  SRR4302540.680263/1‑94 (MQ=60)
                                                                              TATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTG                  <  SRR4302540.6326174/94‑1 (MQ=60)
                                                                               ATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGC                 >  SRR4302540.6832278/1‑94 (MQ=60)
                                                                               ATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGC                 <  SRR4302540.6155587/94‑1 (MQ=60)
                                                                                TTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCC                >  SRR4302540.1662156/1‑94 (MQ=60)
                                                                                 TCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCA               <  SRR4302540.7020668/94‑1 (MQ=60)
                                                                                 TCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCA               <  SRR4302540.222972/94‑1 (MQ=60)
                                                                                  CGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAG              <  SRR4302540.3159926/94‑1 (MQ=60)
                                                                                  CGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAG              >  SRR4302540.5271856/1‑94 (MQ=60)
                                                                                  CGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAG              <  SRR4302540.219961/94‑1 (MQ=60)
                                                                                   GTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGC             >  SRR4302540.4635765/1‑94 (MQ=60)
                                                                                   GTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGC             >  SRR4302540.3467875/1‑94 (MQ=60)
                                                                                   GTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGC             >  SRR4302540.3805974/1‑94 (MQ=60)
                                                                                   GTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGC             >  SRR4302540.4635764/1‑94 (MQ=60)
                                                                                     AAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAGTTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGA           >  SRR4302540.6234518/1‑94 (MQ=60)
                                                                                     AAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGA           <  SRR4302540.5224932/94‑1 (MQ=60)
                                                                                     AAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGA           >  SRR4302540.7678409/1‑94 (MQ=60)
                                                                                     AAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGA           <  SRR4302540.3816024/94‑1 (MQ=60)
                                                                                      AACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGAT          <  SRR4302540.6094570/94‑1 (MQ=60)
                                                                                      AACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGAT          >  SRR4302540.5733211/1‑94 (MQ=60)
                                                                                      AACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGAT          <  SRR4302540.5480008/94‑1 (MQ=60)
                                                                                      AACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGAT          >  SRR4302540.5060277/1‑94 (MQ=60)
                                                                                      AACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGAT          <  SRR4302540.3337596/94‑1 (MQ=60)
                                                                                      AACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGAT          <  SRR4302540.3102999/94‑1 (MQ=60)
                                                                                       ACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATG         >  SRR4302540.1095639/1‑94 (MQ=60)
                                                                                        CGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGG        <  SRR4302540.8057289/94‑1 (MQ=60)
                                                                                         GTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGC       >  SRR4302540.523167/1‑94 (MQ=60)
                                                                                         GTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGC       >  SRR4302540.1880276/1‑94 (MQ=60)
                                                                                            acCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG    >  SRR4302540.8263805/3‑94 (MQ=60)
                                                                                            acCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG    >  SRR4302540.9747761/3‑94 (MQ=60)
                                                                                            acCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG    >  SRR4302540.2326119/3‑94 (MQ=60)
                                                                                            acCTGACGGAAAACGATCGTCGCCGCGCCAACAGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG    <  SRR4302540.10155793/92‑1 (MQ=60)
                                                                                            C‑CTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGT   >  SRR4302540.5038969/1‑94 (MQ=60)
                                                                                            C‑CTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGT   >  SRR4302540.8333568/1‑94 (MQ=60)
                                                                                            C‑CTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGT   >  SRR4302540.1006877/1‑94 (MQ=60)
                                                                                              CTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGTT  >  SRR4302540.2166319/1‑94 (MQ=60)
                                                                                              |                                                                                             
TATCCGCATGACTCTGGAACAAGCTCTGGAGTTCATCGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCA‑CTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGTT  >  NC_000913/4057666‑4057852

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑