Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1548 369.5 20095359 96.9% 19472402 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,435,689 C→T D135D (GAC→GAT)  ytfI → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,435,6890CT99.9% 2700.0 / ‑6.1 726D135D (GAC→GAT) ytfIhypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base T (327/398);  total (328/398)
Fisher's exact test for biased strand distribution p-value = 4.52e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.82e-01

Only 100 of 740 total aligned reads displayed.

TTGAATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAAC‑AAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCGATTGGTGACGTG  >  NC_000913/4435601‑4435778
                                                                                         |                                                                                         
 tGAATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGAtt                                                                                       >  2:3117735/1‑93 (MQ=255)
  gAATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTg                                                                                      >  1:1315008/1‑93 (MQ=255)
  gAATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTg                                                                                      >  2:8662447/1‑93 (MQ=255)
  gAATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTg                                                                                      >  1:8185823/1‑93 (MQ=255)
    aTACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTa                                                                                    >  1:1990264/1‑93 (MQ=255)
    aTACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTa                                                                                    <  1:2491214/93‑1 (MQ=255)
    aTACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTa                                                                                    <  1:7312818/93‑1 (MQ=255)
    aTACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTa                                                                                    <  2:8533430/93‑1 (MQ=255)
      aCGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATg                                                                                  <  2:2076598/93‑1 (MQ=255)
      aCGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATg                                                                                  >  1:6708999/1‑93 (MQ=255)
         aagaaATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCt                                                                               >  1:10008187/1‑93 (MQ=255)
         aagaaATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCt                                                                               <  1:3846703/93‑1 (MQ=255)
          agaaATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTg                                                                              >  2:2660357/1‑93 (MQ=255)
                aCCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGatat                                                                        <  2:1532709/93‑1 (MQ=255)
                aCCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGatat                                                                        >  1:8787019/1‑93 (MQ=255)
                aCCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGatat                                                                        <  1:4723028/93‑1 (MQ=255)
                  ccATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGACTTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTc                                                                      >  1:1529658/1‑93 (MQ=255)
                   cATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCt                                                                     >  2:2565844/1‑93 (MQ=255)
                     tAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTAc                                                                   >  1:974810/1‑93 (MQ=255)
                      aaCAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACa                                                                  >  1:9073827/1‑93 (MQ=255)
                       aCAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAg                                                                 >  1:8322807/1‑93 (MQ=255)
                       aCAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAg                                                                 >  1:7025225/1‑93 (MQ=255)
                       aCAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAg                                                                 >  1:9635553/1‑93 (MQ=255)
                        cAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGa                                                                <  1:2295917/93‑1 (MQ=255)
                        cAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGa                                                                >  1:9975739/1‑93 (MQ=255)
                        cAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGa                                                                <  1:5068530/93‑1 (MQ=255)
                         aaTAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGaa                                                               >  2:5713799/1‑93 (MQ=255)
                           tAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGaaaa                                                             >  2:5388638/1‑93 (MQ=255)
                           tAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGaaaa                                                             >  1:6231396/1‑93 (MQ=255)
                            aaaTGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGaaaaa                                                            <  1:5308660/93‑1 (MQ=255)
                            aaaTGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGaaaaa                                                            >  1:3672541/1‑93 (MQ=255)
                            aaaTGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGaaaaa                                                            >  1:7491223/1‑93 (MQ=255)
                            aaaTGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGaaaaa                                                            >  1:7697799/1‑93 (MQ=255)
                            aaaTGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGaaaaa                                                            >  2:8246021/1‑93 (MQ=255)
                             aaTGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGaaaaaa                                                           <  1:5759713/93‑1 (MQ=255)
                              aTGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAAc                                                          <  2:7684482/93‑1 (MQ=255)
                               tGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACa                                                         <  1:6117423/93‑1 (MQ=255)
                                 aaTATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGt                                                       <  1:7993430/93‑1 (MQ=255)
                                  atatAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTc                                                      <  2:7036847/93‑1 (MQ=255)
                                   tataACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCt                                                     >  2:6630503/1‑93 (MQ=255)
                                   tataACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCt                                                     <  1:2753362/93‑1 (MQ=255)
                                     taACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGa                                                   <  2:6790993/93‑1 (MQ=255)
                                     taACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTATGa                                                   >  2:8408982/1‑93 (MQ=255)
                                     taACTCATTCATGGATTTCAAAAACCGTGAATCTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGa                                                   >  2:7373786/1‑93 (MQ=255)
                                       aCTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACa                                                 >  1:7876216/1‑93 (MQ=255)
                                       aCTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACa                                                 <  1:4045623/93‑1 (MQ=255)
                                       aCTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACa                                                 <  1:3204752/93‑1 (MQ=255)
                                       aCTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACa                                                 <  2:946262/93‑1 (MQ=255)
                                       aCTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACa                                                 <  2:4624055/93‑1 (MQ=255)
                                       aCTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACa                                                 >  2:2835628/1‑93 (MQ=255)
                                         tcattcatGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATa                                               <  1:9191092/93‑1 (MQ=255)
                                         tcattcatGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATa                                               >  1:356313/1‑93 (MQ=255)
                                         tcattcatGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATa                                               >  2:5560039/1‑93 (MQ=255)
                                            ttcatGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCg                                            >  2:396141/1‑93 (MQ=255)
                                            ttcatGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCg                                            >  2:1193572/1‑93 (MQ=255)
                                             tcatGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGa                                           >  1:6356906/1‑93 (MQ=255)
                                             tcatGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGa                                           >  1:4317185/1‑93 (MQ=255)
                                              catGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGaa                                          >  1:9492636/1‑93 (MQ=255)
                                              catGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGaa                                          >  1:3445269/1‑93 (MQ=255)
                                               atGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGaaa                                         <  1:9355193/93‑1 (MQ=255)
                                               atGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGaaa                                         <  1:4925425/93‑1 (MQ=255)
                                                tGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGaaaa                                        >  1:8689416/1‑93 (MQ=255)
                                                tGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGaaaa                                        <  1:6839002/93‑1 (MQ=255)
                                                tGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGaaaa                                        >  1:5468043/1‑93 (MQ=255)
                                                 ggATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAg                                       <  1:6487002/93‑1 (MQ=255)
                                                  gATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAgg                                      <  2:5816529/93‑1 (MQ=255)
                                                  gATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAgg                                      >  2:9820126/1‑93 (MQ=255)
                                                   aTTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGa                                     <  2:305308/93‑1 (MQ=255)
                                                    tttCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGAt                                    <  2:9656748/93‑1 (MQ=255)
                                                      tCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTa                                  >  1:5948219/1‑93 (MQ=255)
                                                       cAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTaa                                 <  2:483289/93‑1 (MQ=255)
                                                        aaaaaCCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTaaa                                <  2:3267190/93‑1 (MQ=255)
                                                         aaaaCCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAg                               <  2:6057421/93‑1 (MQ=255)
                                                          aaaCCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAgg                              <  2:6397901/93‑1 (MQ=255)
                                                          aaaCCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAgg                              <  2:3373708/93‑1 (MQ=255)
                                                           acccGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGt                             <  2:1722689/91‑1 (MQ=255)
                                                            aCCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGtt                            >  2:9082946/1‑93 (MQ=255)
                                                             ccGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTta                           <  2:7819753/93‑1 (MQ=255)
                                                               gTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTtata                         >  2:4778345/1‑93 (MQ=255)
                                                               gTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTtata                         >  2:7954538/1‑93 (MQ=255)
                                                                tGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTatat                        <  2:3664345/93‑1 (MQ=255)
                                                                ggAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTatat                        <  2:7601019/92‑1 (MQ=255)
                                                                   aTTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCa                     <  1:439771/93‑1 (MQ=255)
                                                                     ttCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCAcc                   <  1:1713982/93‑1 (MQ=255)
                                                                      tCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCa                  <  1:4439611/93‑1 (MQ=255)
                                                                      tCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCa                  >  1:4629774/1‑93 (MQ=255)
                                                                         gTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGc               >  2:4431565/1‑93 (MQ=255)
                                                                          ttAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCg              >  2:5046229/1‑93 (MQ=255)
                                                                           tAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCGa             >  1:8524895/1‑93 (MQ=255)
                                                                           tAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCGa             <  2:6850046/93‑1 (MQ=255)
                                                                                caaaacaGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCGATTGGt        <  2:9306525/91‑1 (MQ=255)
                                                                                  aaacaGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCGATTGGTGa      <  1:8891897/93‑1 (MQ=255)
                                                                                  aaacaGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCGATTGGTGa      <  2:2263248/93‑1 (MQ=255)
                                                                                  aaacaGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCGATTGGTGa      <  2:5245257/93‑1 (MQ=255)
                                                                                  aaacaGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCGATTGGTGa      <  1:120660/93‑1 (MQ=255)
                                                                                  aaacaGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCCAAAAGGATTAAAGGTTATATTCACCAGGCGATTGGTGa      <  1:2144572/93‑1 (MQ=255)
                                                                                    acaGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCGATTGGTGACg    <  2:8840155/93‑1 (MQ=255)
                                                                                     caGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCGATTGGTGACgt   >  2:4285548/1‑93 (MQ=255)
                                                                                     caGATGATTGTATGCCTGGGATAGTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCGATTGGTGACgt   <  2:7190878/93‑1 (MQ=255)
                                                                                      aGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCGATTGGTGACgtg  >  1:3027751/1‑93 (MQ=255)
                                                                                         |                                                                                         
TTGAATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAAC‑AAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCGATTGGTGACGTG  >  NC_000913/4435601‑4435778

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 404 total aligned reads displayed.
TTTGAATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAAC‑A‑AA‑CAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCGATTGGTGACGTGTCT  >  NC_000913/4435600‑4435781
                                                                                            |                                                                                            
TTTGAATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAA‑AA‑CAGATGAT                                                                                           <  SRR4302540.2592097/94‑1 (MQ=60)
TTTGAATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAA‑AA‑CAGATGAT                                                                                           <  SRR4302540.9089133/94‑1 (MQ=60)
TTTGAATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAA‑AA‑CAGATGAT                                                                                           >  SRR4302540.8519601/1‑94 (MQ=60)
 TTGAATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAA‑AA‑CAGATGATT                                                                                          >  SRR4302540.7517500/1‑94 (MQ=60)
 TTGAATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAA‑AA‑CAGATGATT                                                                                          <  SRR4302540.7834620/94‑1 (MQ=60)
  TGAATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAA‑AA‑CAGATGATTG                                                                                         <  SRR4302540.6279125/94‑1 (MQ=60)
  TGAATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAA‑AA‑CAGATGATTG                                                                                         <  SRR4302540.6279435/94‑1 (MQ=60)
  TGAATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAA‑AA‑CAGATGATTG                                                                                         >  SRR4302540.6353550/1‑94 (MQ=60)
  TGAATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAA‑AA‑CAGATGATTG                                                                                         >  SRR4302540.1328683/1‑94 (MQ=60)
   GAATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAA‑AA‑CAGATGATTGT                                                                                        >  SRR4302540.3392944/1‑94 (MQ=60)
    AATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAA‑AA‑CAGATGATTGTA                                                                                       >  SRR4302540.9642996/1‑94 (MQ=60)
    AATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAA‑AA‑CAGATGATTGTA                                                                                       >  SRR4302540.2011834/1‑94 (MQ=60)
     ATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAA‑AA‑CAGATGATTGTAT                                                                                      <  SRR4302540.5719417/94‑1 (MQ=60)
     ATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAA‑AA‑CAGATGATTGTAT                                                                                      <  SRR4302540.8405480/94‑1 (MQ=60)
     ATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAA‑AA‑CAGATGATTGTAT                                                                                      <  SRR4302540.8232325/94‑1 (MQ=60)
     ATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAA‑AA‑CAGATGATTGTAT                                                                                      <  SRR4302540.8169242/94‑1 (MQ=60)
     ATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAA‑AA‑CAGATGATTGTAT                                                                                      <  SRR4302540.2518042/94‑1 (MQ=60)
      TACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAA‑AA‑CAGATGATTGTATG                                                                                     >  SRR4302540.6783036/1‑94 (MQ=60)
       ACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAA‑AA‑CAGATGATTGTATGC                                                                                    >  SRR4302540.5863531/1‑94 (MQ=60)
        CGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAA‑AA‑CAGATGATTGTATGCC                                                                                   <  SRR4302540.7285517/94‑1 (MQ=60)
         GAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAA‑AA‑CAGATGATTGTATGCCT                                                                                  <  SRR4302540.6723326/94‑1 (MQ=60)
         GAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAA‑AA‑CAGATGATTGTATGCCT                                                                                  <  SRR4302540.2116890/94‑1 (MQ=60)
         GAAGAAATACCCATAACAATAAATGAACATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAA‑AA‑CAGATGATTGTATGCCT                                                                                  <  SRR4302540.2406764/94‑1 (MQ=60)
          AAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAA‑AA‑CAGATGATTGTATGCCTG                                                                                 <  SRR4302540.1305469/94‑1 (MQ=60)
          AAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAA‑AA‑CAGATGATTGTATGCCTG                                                                                 <  SRR4302540.3211308/94‑1 (MQ=60)
             AAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAA‑AA‑CAGATGATTGTATGCCTGGGA                                                                              >  SRR4302540.2149655/1‑94 (MQ=60)
              AATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAA‑AA‑CAGATGATTGTATGCCTGGGAT                                                                             >  SRR4302540.3689446/1‑94 (MQ=60)
               ATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAA‑AA‑CAGATGATTGTATGCCTGGGATA                                                                            >  SRR4302540.3026083/1‑94 (MQ=60)
                TCCCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAA‑AA‑CAGATGATTGTATGCCTGGGATAT                                                                           <  SRR4302540.4315483/94‑1 (MQ=60)
                TACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAA‑AA‑CAGATGATTGTATGCCTGGGATAT                                                                           >  SRR4302540.8888701/1‑94 (MQ=60)
                 ACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAA‑AA‑CAGATGATTGTATGCCTGGGATATT                                                                          <  SRR4302540.4773473/94‑1 (MQ=60)
                 ACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAA‑AA‑CAGATGATTGTATGCCTGGGATATT                                                                          <  SRR4302540.8021542/94‑1 (MQ=60)
                  CCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGACTTTCCGTTAGAACAA‑AA‑CAGATGATTGTATGCCTGGGATATTC                                                                         >  SRR4302540.1546596/1‑94 (MQ=60)
                  CCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAA‑AA‑CAGATGATTGTATGCCTGGGATATTC                                                                         >  SRR4302540.6968441/1‑94 (MQ=60)
                   CCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAA‑AA‑CAGATGATTGTATGCCTGGGATATTCT                                                                        <  SRR4302540.2946519/94‑1 (MQ=60)
                   CCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAA‑AA‑CAGATGATTGTATGCCTGGGATATTCT                                                                        <  SRR4302540.9511308/94‑1 (MQ=60)
                   CCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAA‑AA‑CAGATGATTGTATGCCTGGGATATTCT                                                                        >  SRR4302540.9592910/1‑94 (MQ=60)
                    CATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAA‑AA‑CAGATGATTGTATGCCTGGGATATTCTA                                                                       >  SRR4302540.7039291/1‑94 (MQ=60)
                    CATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAA‑AA‑CAGATGATTGTATGCCTGGGATATTCTA                                                                       >  SRR4302540.3311192/1‑94 (MQ=60)
                     ATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAA‑AA‑CAGATGATTGTATGCCTGGGATATTCTAC                                                                      <  SRR4302540.3500548/94‑1 (MQ=60)
                     ATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACAGTGAATTTCCGTTAGAACAA‑AA‑AAGATGATTGTATGCCTGGGGTATTCTAC                                                                      >  SRR4302540.8603317/1‑94 (MQ=60)
                      TAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAA‑AA‑CAGATGATTGTATGCCTGGGATATTCTACA                                                                     >  SRR4302540.9182800/1‑94 (MQ=60)
                       AACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAA‑AA‑CAGATGATTGTATGCCTGGGATATTCTACTG                                                                    >  SRR4302540.8807884/1‑94 (MQ=60)
                       AACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAA‑AA‑CAGATGATTGTATGCCTGGGATATTCTACAG                                                                    >  SRR4302540.7103266/1‑94 (MQ=60)
                       AACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAA‑AA‑CAGATGATTGTATGCCTGGGATATTCTACAG                                                                    <  SRR4302540.7335081/94‑1 (MQ=60)
                       AACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAA‑AA‑CAGATGATTGTATGCCTGGGATATTCTACAG                                                                    >  SRR4302540.8419407/1‑94 (MQ=60)
                       AACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAAC‑ACAA‑CAGATGATTGTATGCCTGGGATATTCTACAG                                                                    >  SRR4302540.9797550/1‑94 (MQ=60)
                        ACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAA‑AA‑CAGATGATTGTATGCCTGGGATATTCTACAGA                                                                   <  SRR4302540.6511686/94‑1 (MQ=60)
                        ACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAA‑AA‑CAGATGATTGTATGCCTGGGATATTCTACAGA                                                                   >  SRR4302540.4461134/1‑94 (MQ=60)
                        ACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAA‑AA‑CAGATGATTGTATGCCTGGGATATTCTACAGA                                                                   >  SRR4302540.10092079/1‑94 (MQ=60)
                        ACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAA‑AA‑CAGATGATTGTATGCCTGGGATATTCTACAGA                                                                   <  SRR4302540.2285534/94‑1 (MQ=60)
                         CAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAA‑AA‑CAGATGATTGTATGCCTGGGATATTCTACAGAA                                                                  >  SRR4302540.2049241/1‑94 (MQ=60)
                         CAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAA‑AA‑CAGATGATTGTATGCCTGGGATATTCTACAGAA                                                                  <  SRR4302540.5123329/94‑1 (MQ=60)
                         CAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAA‑AA‑CAGATGATTGTATGCCTGGGATATTCTACAGAA                                                                  <  SRR4302540.2320628/94‑1 (MQ=60)
                          TATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAA‑AA‑CAGATGATTGTATGCCTGGGATATTCTACAGAAA                                                                 >  SRR4302540.5409443/1‑94 (MQ=60)
                          AATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAA‑AA‑CAGATGATTGTATGCCTGGGATATTCTACAGAAA                                                                 <  SRR4302540.3932389/94‑1 (MQ=60)
                          AATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAA‑AA‑CAGATGATTGTATGCCTGGGATATTCTACACAAA                                                                 >  SRR4302540.2881339/1‑94 (MQ=60)
                           ATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAA‑AA‑CAGATGATTGTATGCCTGGGATATTCTACAGAAAA                                                                >  SRR4302540.7533030/1‑94 (MQ=60)
                           ATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAA‑AA‑CAGATGATTGTATGCCTGGGATATTCTACAGAAAA                                                                >  SRR4302540.1214486/1‑94 (MQ=60)
                           ATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAA‑AA‑CAGATGATTGTATGCCTGGGATATTCTACAGAAAA                                                                >  SRR4302540.8957223/1‑94 (MQ=60)
                            TAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAA‑AA‑CAGATGATTGTATGCCTGGGATATTCTACAGAAAAA                                                               >  SRR4302540.7574862/1‑94 (MQ=60)
                            TAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAA‑AA‑CAGATGATTGTATGCCTGGGATATTCTACAGAAAAA                                                               <  SRR4302540.44920/94‑1 (MQ=60)
                            TAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAA‑AA‑CAGATGATTGTATGCCTGGGATATTCTACAGAAAAA                                                               >  SRR4302540.9494560/1‑94 (MQ=60)
                            TAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAA‑AA‑CAGATGATTGTATGCCTGGGATATTCTACAGAAAAA                                                               >  SRR4302540.7785596/1‑94 (MQ=60)
                            TAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAA‑AA‑CAGATGATTGTATGCCTGGGATATTCTACAGAAAAA                                                               <  SRR4302540.7885034/94‑1 (MQ=60)
                             AAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAA‑AA‑CAGATGATTGTATGCCTGGGATATTCTACAGAAAAAA                                                              >  SRR4302540.2812997/1‑94 (MQ=60)
                             AAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAA‑AA‑CAGATGATTGTATGCCTGGGATATTCTACAGAAAAAA                                                              <  SRR4302540.4016624/94‑1 (MQ=60)
                             AAATGAATATAACTCATTCATGGATTTCAAAAACCGGGAATTTCCGTTAGAAC‑AGAA‑CAGATGATTGTATGCCTGGGATATTCTACAGAAAcac                                                              >  SRR4302540.6830282/1‑91 (MQ=60)
                              AATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAA‑AA‑CAGATGATTGTATGCCTGGGATATTCTACAGAAAAAAC                                                             >  SRR4302540.8187640/1‑94 (MQ=60)
                               ATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAA‑AA‑CAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACA                                                            <  SRR4302540.2193459/94‑1 (MQ=60)
                               ATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAA‑AA‑CAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACA                                                            >  SRR4302540.4711077/1‑94 (MQ=60)
                                TGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAA‑AA‑CAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAG                                                           >  SRR4302540.1265412/1‑94 (MQ=60)
                                TGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAA‑AA‑CAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAG                                                           <  SRR4302540.6185552/94‑1 (MQ=60)
                                 GAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAA‑AA‑CAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGT                                                          >  SRR4302540.5107313/1‑94 (MQ=60)
                                 GAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAA‑AA‑CAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGT                                                          >  SRR4302540.6324875/1‑94 (MQ=60)
                                 GAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAA‑AA‑CAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGT                                                          <  SRR4302540.8591393/94‑1 (MQ=60)
                                  AATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAA‑AA‑CAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTC                                                         >  SRR4302540.4396631/1‑94 (MQ=60)
                                  AATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAA‑AA‑CAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTC                                                         >  SRR4302540.10135117/1‑94 (MQ=60)
                                  AATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAA‑AA‑CAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTC                                                         <  SRR4302540.8086485/94‑1 (MQ=60)
                                  AATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAA‑AA‑CAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTC                                                         <  SRR4302540.8129462/94‑1 (MQ=60)
                                   ATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAA‑AA‑CAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCT                                                        >  SRR4302540.3832783/1‑94 (MQ=60)
                                   ATATAACTCATTCAGAGATTGCAAAACCCGTGAATTTCCGTTAGAACAA‑AA‑CAGATGAGTGTATGCCTGGGATATTCTACAGAAAAAACAGTCT                                                        <  SRR4302540.9369442/94‑1 (MQ=60)
                                    TATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAA‑AA‑CAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTG                                                       >  SRR4302540.8078880/1‑94 (MQ=60)
                                    TATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAA‑AA‑CAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTG                                                       <  SRR4302540.5331199/94‑1 (MQ=60)
                                    TATAACTCATTCATGGATTTCAAAAACCGTGAAGTTCCGTTAGAACAA‑AA‑CAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTG                                                       <  SRR4302540.4961040/94‑1 (MQ=60)
                                     ATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAA‑AA‑CAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGA                                                      >  SRR4302540.8990493/1‑94 (MQ=60)
                                      TAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAA‑AA‑CAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGAC                                                     <  SRR4302540.1124122/94‑1 (MQ=60)
                                       AACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAA‑AA‑CAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACA                                                    >  SRR4302540.8694761/1‑94 (MQ=60)
                                       AACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAA‑AA‑CAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACA                                                    <  SRR4302540.6032/94‑1 (MQ=60)
                                       AACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAA‑AA‑CAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACA                                                    >  SRR4302540.3484607/1‑94 (MQ=60)
                                       AACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAA‑AA‑CAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACA                                                    >  SRR4302540.7968208/1‑94 (MQ=60)
                                       AACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAA‑AA‑CAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACA                                                    <  SRR4302540.1998511/94‑1 (MQ=60)
                                        ACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAA‑AA‑CAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACAT                                                   <  SRR4302540.7811446/94‑1 (MQ=60)
                                        ACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAA‑AA‑CAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACAT                                                   <  SRR4302540.4089244/94‑1 (MQ=60)
                                        ACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAA‑AA‑CAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACAT                                                   <  SRR4302540.3239556/94‑1 (MQ=60)
                                        ACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAA‑AA‑CAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACAT                                                   <  SRR4302540.3662112/94‑1 (MQ=60)
                                        ACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAA‑AA‑CAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACAT                                                   <  SRR4302540.4628528/94‑1 (MQ=60)
                                        ACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAA‑AA‑CAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACAT                                                   <  SRR4302540.4736097/94‑1 (MQ=60)
                                         CTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAA‑AA‑CAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATA                                                  >  SRR4302540.359977/1‑94 (MQ=60)
                                         CTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAA‑AA‑CAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATA                                                  <  SRR4302540.4054938/94‑1 (MQ=60)
                                         CTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAA‑AA‑CAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATA                                                  <  SRR4302540.1423702/94‑1 (MQ=60)
                                         CTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAA‑AA‑CAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATA                                                  >  SRR4302540.5385940/1‑94 (MQ=60)
                                          TCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAA‑AA‑CAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATAC                                                 <  SRR4302540.9850786/94‑1 (MQ=60)
                                          TCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAA‑AA‑CAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATAC                                                 <  SRR4302540.5003630/94‑1 (MQ=60)
                                          TCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAA‑AA‑CAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATAC                                                 >  SRR4302540.6312602/1‑94 (MQ=60)
                                          TCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAA‑AA‑CAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATAC                                                 <  SRR4302540.9337617/94‑1 (MQ=60)
                                          TCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAA‑AA‑CAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATAC                                                 <  SRR4302540.9301321/94‑1 (MQ=60)
                                          TCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAA‑AA‑CAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATAC                                                 <  SRR4302540.9766428/94‑1 (MQ=60)
                                          TCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAA‑AA‑CAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATAC                                                 >  SRR4302540.8637957/1‑94 (MQ=60)
                                           CATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAA‑AA‑CAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACC                                                <  SRR4302540.335035/94‑1 (MQ=60)
                                           CATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAA‑AA‑CAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACC                                                >  SRR4302540.447931/1‑94 (MQ=60)
                                            ATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAA‑AA‑CAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCG                                               <  SRR4302540.9138417/94‑1 (MQ=60)
                                            ATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAA‑AA‑CAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCG                                               >  SRR4302540.4983569/1‑94 (MQ=60)
                                            ATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAA‑AA‑CAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCG                                               >  SRR4302540.4833589/1‑94 (MQ=60)
                                            ATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAA‑AA‑CAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCG                                               >  SRR4302540.5791611/1‑94 (MQ=60)
                                            ATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAA‑AA‑CAGATGATTGTATGCATGGGATATTCTACAGAAAAAACAGTCTGACATACCG                                               <  SRR4302540.5538938/94‑1 (MQ=60)
                                             TTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAA‑AA‑CAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGA                                              <  SRR4302540.3822625/94‑1 (MQ=60)
                                             TTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAA‑AA‑CAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGA                                              >  SRR4302540.4364184/1‑94 (MQ=60)
                                             TTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAA‑AA‑CAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGA                                              >  SRR4302540.6427694/1‑94 (MQ=60)
                                              TCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAA‑AA‑CAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAA                                             >  SRR4302540.5266774/1‑94 (MQ=60)
                                              TCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAA‑AA‑CAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAA                                             >  SRR4302540.9605153/1‑94 (MQ=60)
                                              TCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAA‑AA‑CAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAA                                             >  SRR4302540.9241356/1‑94 (MQ=60)
                                              TCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAA‑AA‑CAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAA                                             >  SRR4302540.8800026/1‑94 (MQ=60)
                                               CATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAA‑AA‑CAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAA                                            <  SRR4302540.6447239/94‑1 (MQ=60)
                                               CATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAA‑AA‑CAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAA                                            >  SRR4302540.2717913/1‑94 (MQ=60)
                                                ATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAA‑AA‑CAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAA                                           >  SRR4302540.7174995/1‑94 (MQ=60)
                                                ATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAA‑AA‑CAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAA                                           >  SRR4302540.2412686/1‑94 (MQ=60)
                                                ATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAA‑AA‑CAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAA                                           <  SRR4302540.9466614/94‑1 (MQ=60)
                                                ATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAA‑AA‑CAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAA                                           >  SRR4302540.9009114/1‑94 (MQ=60)
                                                ATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAA‑AA‑CAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAA                                           >  SRR4302540.1389700/1‑94 (MQ=60)
                                                 TGGATTTCAAAAACCGTGAATTTCCGTTAGAACAA‑AA‑CAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAG                                          <  SRR4302540.5250749/94‑1 (MQ=60)
                                                 TGGATTTCAAAAACCGTGAATTTCCGTTAGAACAA‑AA‑CAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAG                                          <  SRR4302540.2489028/94‑1 (MQ=60)
                                                  GGATTTCAAAAACCGTGAATTTCCGTTAGAACAA‑AA‑CAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGG                                         >  SRR4302540.3571022/1‑94 (MQ=60)
                                                  GGATTTCAAAAACCGTGAATTTCCGTTAGAACAA‑AA‑CAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGG                                         >  SRR4302540.6909108/1‑94 (MQ=60)
                                                  GGATTTCAAAAACCGTGAATTTCCGTTAGAACAA‑AA‑CAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGG                                         <  SRR4302540.6559145/94‑1 (MQ=60)
                                                  GGATTTCAAAAACCGTGAATTTCCGTTAGAACAA‑AA‑CAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGG                                         >  SRR4302540.6580586/1‑94 (MQ=60)
                                                   GATTTCAAAAACCGTGAATTTCCGTTAGAACAA‑AA‑CAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGA                                        >  SRR4302540.9231978/1‑94 (MQ=60)
                                                   GATTTCAAAAACCGTGAATTTCCGTTAGAACAA‑AA‑CAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGA                                        <  SRR4302540.4364797/94‑1 (MQ=60)
                                                    ATTTCAAAAACCGTGAATTTCCGTTAGAACAA‑AA‑CAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGAT                                       >  SRR4302540.2268462/1‑94 (MQ=60)
                                                    ATTTCAAAAACCGTGAATTTCCGTTAGAACAA‑AA‑CAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGAT                                       <  SRR4302540.5445371/94‑1 (MQ=60)
                                                    ATTTCAAAAACCGTGAATTTCCGTTAGAACAA‑AA‑CAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACAGAAAAGGAT                                       >  SRR4302540.1830645/1‑94 (MQ=60)
                                                     TTTCAAAAACCGTGAATTTCCGTTAGAACAA‑AA‑CAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATT                                      >  SRR4302540.866551/1‑94 (MQ=60)
                                                     TTTCAAAAACCGTGAATTTCCGTTAGAACAA‑AA‑CAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATT                                      >  SRR4302540.7757148/1‑94 (MQ=60)
                                                     TTTCAAAAACCGTGAATTTCCGTTAGAACAA‑AA‑CAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATT                                      >  SRR4302540.7082735/1‑94 (MQ=60)
                                                      TTCAAAAACCGTGAATTTCCGTTAGAACAA‑AA‑CAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTA                                     >  SRR4302540.6013989/1‑94 (MQ=60)
                                                       TCAAAAACCGTGAATTTCCGTTAGAACAA‑AA‑CAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAA                                    <  SRR4302540.4248777/94‑1 (MQ=60)
                                                       TCAAAAACCGTGAATTTCCGTTAGAACAA‑AA‑CAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAA                                    <  SRR4302540.6130208/94‑1 (MQ=60)
                                                        CAAAAACCGTGAATTTCCGTTAGAACAA‑AA‑CAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAA                                   <  SRR4302540.9253434/94‑1 (MQ=60)
                                                         AAAAACCGTGAATTTCCGTTAGAACAA‑AA‑CAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAG                                  >  SRR4302540.641497/1‑94 (MQ=60)
                                                         AAAAACCGTGAATTTCCGTTAGAACAA‑AA‑CAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAG                                  <  SRR4302540.4886314/94‑1 (MQ=60)
                                                         AAAAACCGTGAATTTCCGTTAGAACAA‑AA‑CAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAG                                  <  SRR4302540.5437270/94‑1 (MQ=60)
                                                           AAACCGTGAATTTCCGTTAGAACAA‑AA‑CAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGT                                <  SRR4302540.6212520/94‑1 (MQ=60)
                                                            AACCGTGAATTTCCGTTAGAACAA‑AA‑CAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTT                               >  SRR4302540.9826829/1‑94 (MQ=60)
                                                              CCGTGAATTTCCGTTAGAACAA‑AA‑CAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTAT                             >  SRR4302540.8350809/1‑94 (MQ=60)
                                                              CCGTGAATTTCCGTTAGAACAA‑AA‑CAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTAT                             >  SRR4302540.7738826/1‑94 (MQ=60)
                                                               CGTGAATTTCCGTTAGAACAA‑AA‑CAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATA                            >  SRR4302540.6888500/1‑94 (MQ=60)
                                                                GTGAATTTCCGTTAGAACAA‑AA‑CAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATAT                           <  SRR4302540.4412568/94‑1 (MQ=60)
                                                                GTGAATTTCCGTTAGAACAA‑AA‑CAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATAT                           <  SRR4302540.9723901/94‑1 (MQ=60)
                                                                 TGAATTTCCGTTAGAACAA‑AA‑CAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATT                          <  SRR4302540.9684545/94‑1 (MQ=60)
                                                                  GAATTTCCGTTAGAACAA‑AA‑CAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTC                         <  SRR4302540.10048901/94‑1 (MQ=60)
                                                                  GAATTTCCGTTAGAACAA‑AA‑CAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTC                         <  SRR4302540.5046218/94‑1 (MQ=60)
                                                                  GAATTTCCGTTAGAACAA‑AA‑CAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTC                         <  SRR4302540.8784627/94‑1 (MQ=60)
                                                                  GAATTTCCGTTAGAACAA‑AA‑CAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTC                         <  SRR4302540.8363795/94‑1 (MQ=60)
                                                                   AATTTCCGTTAGAACAA‑AA‑CAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCA                        <  SRR4302540.7400773/94‑1 (MQ=60)
                                                                    ATTTCCGTTAGAACAA‑AA‑CAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCAC                       <  SRR4302540.444449/94‑1 (MQ=60)
                                                                    ATTTCCGTTAGAACAA‑AA‑CAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCAC                       <  SRR4302540.3081339/94‑1 (MQ=60)
                                                                      TTCCGTTAGAACAA‑AA‑CAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCA                     >  SRR4302540.9900899/1‑94 (MQ=60)
                                                                      TTCCGTTAGAACAA‑AA‑CAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCA                     <  SRR4302540.7359636/94‑1 (MQ=60)
                                                                      TTCCGTTAGAACAA‑AA‑CAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCA                     <  SRR4302540.6756680/94‑1 (MQ=60)
                                                                      TTCCGTTAGAACAA‑AA‑CAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCA                     >  SRR4302540.4679369/1‑94 (MQ=60)
                                                                       TCCGTTAGAACAA‑AA‑CAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAG                    <  SRR4302540.2645773/94‑1 (MQ=60)
                                                                       TCCGTTAGAACAA‑AA‑CAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAG                    <  SRR4302540.6054590/94‑1 (MQ=60)
                                                                       TCCGTTAGAACAA‑AA‑CAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAG                    <  SRR4302540.5985457/94‑1 (MQ=60)
                                                                        CCGTTAGAACAA‑AA‑CAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGG                   <  SRR4302540.161391/94‑1 (MQ=60)
                                                                        CCGTTAGAACAA‑AA‑CAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGG                   <  SRR4302540.4654212/94‑1 (MQ=60)
                                                                          GTTAGAACAA‑AA‑CAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCG                 <  SRR4302540.1694373/94‑1 (MQ=60)
                                                                          GTTAGAACAA‑AA‑CAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCG                 >  SRR4302540.1821856/1‑94 (MQ=60)
                                                                            TAGAACAA‑AA‑CAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCGAT               <  SRR4302540.3705106/94‑1 (MQ=60)
                                                                             AGAACAA‑AA‑CAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACaagcggttg              >  SRR4302540.189772/1‑85 (MQ=60)
                                                                             AGAACAA‑AA‑CAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCGATT              >  SRR4302540.2689837/1‑94 (MQ=60)
                                                                             AGAACAA‑AA‑CAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCGATT              <  SRR4302540.1569468/94‑1 (MQ=60)
                                                                             AGAACAA‑AA‑CAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCGATT              <  SRR4302540.388487/94‑1 (MQ=60)
                                                                             AGAAC‑A‑AACCAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCCCCAGGCGATT              <  SRR4302540.4514976/94‑1 (MQ=60)
                                                                               AACAA‑AA‑CAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCGATTGG            <  SRR4302540.316182/94‑1 (MQ=60)
                                                                                acaA‑AA‑CAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCGATTGGT           >  SRR4302540.8854030/4‑94 (MQ=60)
                                                                                 A‑A‑AA‑CAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCGATTGGTGA         >  SRR4302540.446858/1‑94 (MQ=60)
                                                                                   A‑AA‑CAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCGATTGGTGAC        <  SRR4302540.1509359/94‑1 (MQ=60)
                                                                                   A‑AA‑CAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCGATTGGTGAC        <  SRR4302540.8994829/94‑1 (MQ=60)
                                                                                   A‑AA‑CAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCGATTGGTGAC        <  SRR4302540.1483922/94‑1 (MQ=60)
                                                                                   A‑AA‑CAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCGATTGGTGAC        <  SRR4302540.3763185/94‑1 (MQ=60)
                                                                                   A‑AA‑CAGATGATTGTATGCCTGGGATATTCCACAGAAAAACCAGTCTGACATCCCGAAAAGGATTAAAGGTTATATTCACCAGGCGATTGGTGAC        <  SRR4302540.121957/94‑1 (MQ=60)
                                                                                     AA‑CAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCGATTGGTGACG       <  SRR4302540.4420953/94‑1 (MQ=60)
                                                                                     AA‑CAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCGATTGGTGACG       >  SRR4302540.592302/1‑94 (MQ=60)
                                                                                        CAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCGATTGGTGACGTG     >  SRR4302540.8896767/1‑94 (MQ=60)
                                                                                        CAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCGATTGGTGACGTG     >  SRR4302540.9397791/1‑94 (MQ=60)
                                                                                         AGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCGATTGGTGACGTGT    >  SRR4302540.9674291/1‑94 (MQ=60)
                                                                                          GATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCGATTGGTGACGTGTC   >  SRR4302540.7671978/1‑94 (MQ=60)
                                                                                          GATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCGATTGGTGACGTGTC   <  SRR4302540.5498858/94‑1 (MQ=60)
                                                                                           ATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCGATTGGTGACGTGTCT  <  SRR4302540.1089068/94‑1 (MQ=60)
                                                                                           ATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCGATTGGTGACGTGTCT  <  SRR4302540.6845515/94‑1 (MQ=60)
                                                                                            |                                                                                            
TTTGAATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAAC‑A‑AA‑CAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCGATTGGTGACGTGTCT  >  NC_000913/4435600‑4435781

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑