Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A0 F0 I1 R1 245 579.6 28867798 97.7% 28203838 96.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,971,075 2 bp→GC coding (942‑943/1068 nt) rffG → dTDP‑glucose 4,6‑dehydratase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,971,0750CG99.4% 1778.4 / NA 462C314W (TGC→TGG) rffGdTDP‑glucose 4,6‑dehydratase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base G (241/217);  minor base T (0/3);  total (241/221)
Fisher's exact test for biased strand distribution p-value = 1.08e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*NC_0009133,971,0760GC99.8% 1806.9 / ‑5.0 461V315L (GTG→CTG) rffGdTDP‑glucose 4,6‑dehydratase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base C (238/221);  minor base T (1/0);  total (240/221)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.29e-01

Only 100 of 488 total aligned reads displayed.

TCGTGACCTGATCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGCGTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAG  >  NC_000913/3970985‑3971167
                                                                                          ||                                                                                           
tCGTGGCCTGATCACCTTTGTCGCTGACCGTCCTGGGCATGATCTGCGTTATGCCATTGATGCTCCGAAAATTGCCCGTGAACTTGGCTGGCTGcc                                                                                         <  1:9515938/96‑1 (MQ=255)
 cGTGACCTGATCACCTTTGTCGCTGACCGTTCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCg                                                                                        >  1:712348/1‑96 (MQ=255)
 cGTGACCTGATCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCg                                                                                        <  2:12308474/96‑1 (MQ=255)
  gTGACCTGATCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGccac                                                                                       <  1:3388780/96‑3 (MQ=255)
  gTGACCTGATCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGc                                                                                       >  1:112728/1‑96 (MQ=255)
     aCCTGATCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAgg                                                                                    >  2:2204372/1‑96 (MQ=255)
      ccTGATCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGa                                                                                   <  2:10873401/96‑1 (MQ=255)
      ccTGATCACCTTTGGCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGa                                                                                   >  2:1908637/1‑96 (MQ=255)
        tGATCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGaaa                                                                                 <  2:1434391/96‑1 (MQ=255)
        tGATCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGaaa                                                                                 >  1:479188/1‑96 (MQ=255)
          aTCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGGTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAAcc                                                                               >  1:1445240/1‑96 (MQ=255)
           tCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAACATTGCCCGTGACCTTGGCTGGCTGCCGCAGGAAACCt                                                                              <  1:5259875/96‑1 (MQ=255)
            cACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCtt                                                                             >  2:7456253/1‑96 (MQ=255)
             aCCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCttt                                                                            <  2:6148873/96‑1 (MQ=255)
             aCCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCttt                                                                            <  2:13528472/96‑1 (MQ=255)
               cTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGa                                                                          >  1:4517810/1‑96 (MQ=255)
                tttGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGaa                                                                         >  1:7297044/1‑96 (MQ=255)
                tttGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGaa                                                                         >  1:2444976/1‑96 (MQ=255)
                 ttGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGaaa                                                                        >  2:3980405/1‑96 (MQ=255)
                  tGTCGCTGACCGTCCGGGGCATGATCTGCGGTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCCGGAAACCTTTGAAAg                                                                       >  1:5683455/1‑96 (MQ=255)
                   gTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGt                                                                      <  1:6373427/96‑1 (MQ=255)
                    tCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGACCTTGGCTGGCTGCCGCAGGAAGCCTTTGAAAGTg                                                                     <  2:3286647/96‑1 (MQ=255)
                    tCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTg                                                                     <  2:8142104/96‑1 (MQ=255)
                    tCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTg                                                                     <  2:8493151/96‑1 (MQ=255)
                     cGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGtcg                                                                    <  1:7660796/96‑3 (MQ=255)
                     cGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTgg                                                                    <  1:3127272/96‑1 (MQ=255)
                     cGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTgg                                                                    <  2:938896/96‑1 (MQ=255)
                      gCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGa                                                                   >  2:12588466/1‑96 (MQ=255)
                        tGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGTAAt                                                                 >  1:3950116/1‑96 (MQ=255)
                        tGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAAt                                                                 <  1:5027180/96‑1 (MQ=255)
                        tGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAAt                                                                 >  2:13864546/1‑96 (MQ=255)
                        tGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAAt                                                                 >  1:10040750/1‑96 (MQ=255)
                        tGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAAt                                                                 >  2:7795891/1‑96 (MQ=255)
                            cGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGGAAGTGGAATGAGt                                                             >  1:1585007/1‑96 (MQ=255)
                             gTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCgca                                                            <  1:10833343/96‑3 (MQ=255)
                             gTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTa                                                            <  1:1948170/96‑1 (MQ=255)
                              tCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTaa                                                           >  1:6759713/1‑96 (MQ=255)
                                 ggggCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGTTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAAc                                                        >  2:4779150/1‑96 (MQ=255)
                                 ggggCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAAc                                                        <  2:2555464/96‑1 (MQ=255)
                                 ggggCATGATCTGCGTTATGCCATTGATGCCTCGAAAATTGCCCGTGACCTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAAc                                                        <  2:14117401/96‑1 (MQ=255)
                                  gggCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACg                                                       <  1:2258862/96‑1 (MQ=255)
                                  gggCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACg                                                       <  1:11545417/96‑1 (MQ=255)
                                    gCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGt                                                     <  2:6449711/96‑1 (MQ=255)
                                    gCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGt                                                     >  2:119468/1‑96 (MQ=255)
                                     cATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGtt                                                    <  2:4176545/96‑1 (MQ=255)
                                      aTGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTc                                                   <  2:13734395/96‑1 (MQ=255)
                                      aTGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTc                                                   <  1:2647866/96‑1 (MQ=255)
                                       tGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCa                                                  >  2:3151582/1‑96 (MQ=255)
                                       tGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCa                                                  >  2:7279130/1‑96 (MQ=255)
                                        gATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAg                                                 >  1:12009798/1‑96 (MQ=255)
                                        gATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAg                                                 <  2:10331500/96‑1 (MQ=255)
                                          tCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTg                                               <  2:7537989/96‑1 (MQ=255)
                                             gCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTggca                                            <  1:10272930/96‑3 (MQ=255)
                                             gCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTa                                            <  2:10136996/96‑1 (MQ=255)
                                              cGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTAt                                           >  2:1152734/1‑96 (MQ=255)
                                                ttATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCt                                         >  2:13297006/1‑96 (MQ=255)
                                                 tATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTg                                        <  1:11827444/96‑1 (MQ=255)
                                                  aTGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTgg                                       >  1:5920235/1‑96 (MQ=255)
                                                  aTGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTgg                                       >  2:5313271/1‑96 (MQ=255)
                                                   tGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGc                                      <  1:7790797/96‑1 (MQ=255)
                                                    gCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTggtt                                     <  1:3462625/96‑3 (MQ=255)
                                                    gCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCt                                     <  1:2136884/96‑1 (MQ=255)
                                                    gCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCt                                     >  1:12317518/1‑96 (MQ=255)
                                                     cccttGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGCAAAACGGTTCAGTGGTATCTGGCTa                                    <  1:9237989/93‑1 (MQ=255)
                                                     ccATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTa                                    <  1:13422552/96‑1 (MQ=255)
                                                      cATTGATGCTTCTAAAATTTCCCGTGAACTTGGCTGTCTGCCTCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTaa                                   <  1:7971378/96‑1 (MQ=255)
                                                       aTTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTAAGTGGTATCTGGCTAAt                                  >  2:7001142/1‑96 (MQ=255)
                                                         tGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTCTCTGGCGAATGa                                >  1:9087054/1‑96 (MQ=255)
                                                         tGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGa                                <  2:9607267/96‑1 (MQ=255)
                                                          gATGCTTCGAAAACTGCCCGTGAACTTGGCTGTCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGaa                               <  1:8416593/96‑1 (MQ=255)
                                                             gCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGc                            >  1:9664818/1‑96 (MQ=255)
                                                             gCTTCGAAAACTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAGTGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGc                            <  2:13175584/96‑1 (MQ=255)
                                                              cTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCt                           >  1:6211655/1‑96 (MQ=255)
                                                               ttCGGAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGGATCTGGCTAATGaaaacag                          >  1:10632340/1‑92 (MQ=255)
                                                                 cGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCtggt                        >  2:9103081/1‑96 (MQ=255)
                                                                  ggaaaTTGCCCGGGAACTTGGCTGGCTGCCGCAGGAAACCTGTGAAAGGGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAAGGACAGCtggtg                       >  2:8832688/3‑96 (MQ=255)
                                                                   aaaaTTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCtggtgg                      >  2:603577/1‑96 (MQ=255)
                                                                    aaaTTGCCCGTAAAATTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGa                     <  1:1367780/96‑1 (MQ=255)
                                                                     aaTTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGaa                    <  2:5891778/96‑1 (MQ=255)
                                                                      aTTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAg                   <  2:12444530/96‑1 (MQ=255)
                                                                        tGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCa                 <  1:3559628/96‑1 (MQ=255)
                                                                         gCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAg                >  2:10519819/1‑96 (MQ=255)
                                                                         gCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAg                >  1:3001378/1‑96 (MQ=255)
                                                                          cccGTGAACTTGGCTGTCTGCCGCAGGAACCCTTTGAAAGTGGAATGCGTAAAACGGTGCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCaag               <  1:9884233/96‑3 (MQ=255)
                                                                           ccGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGt              <  2:11830186/96‑1 (MQ=255)
                                                                            cGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTg             <  1:8648124/96‑1 (MQ=255)
                                                                             gTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGc            <  2:502161/96‑1 (MQ=255)
                                                                               gAACTTGGCTGGCTGCCGCAGGAAGCCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAg          <  1:13201991/96‑1 (MQ=255)
                                                                               gAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAg          <  1:824364/96‑1 (MQ=255)
                                                                               gAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGGGGAAGCAGGTGcgg          >  1:5473007/1‑94 (MQ=255)
                                                                                aaCTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAagcaggagcagg         >  2:3731083/1‑96 (MQ=255)
                                                                                aaCTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAgg         <  2:11307958/96‑1 (MQ=255)
                                                                                aaCTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAgg         <  1:426852/96‑1 (MQ=255)
                                                                                aaCTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGCAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAgg         >  1:12937173/1‑96 (MQ=255)
                                                                                 aCTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGGATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGa        >  1:14033808/1‑96 (MQ=255)
                                                                                  cTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGAc       >  1:12487981/1‑96 (MQ=255)
                                                                                  cTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGAc       <  1:654861/96‑1 (MQ=255)
                                                                                   ttGGCTGGCTGCCGCAGGAACCCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACg      <  1:6890621/96‑1 (MQ=255)
                                                                                    tggctggcTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACgg     >  2:3616294/1‑96 (MQ=255)
                                                                                     ggctggctGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGc    <  2:5467410/96‑1 (MQ=255)
                                                                                          ||                                                                                           
TCGTGACCTGATCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGCGTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAG  >  NC_000913/3970985‑3971167

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 274 total aligned reads displayed.
TGGCGCACTATCGTGACCTGATCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGCGTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAG  >  NC_000913/3970975‑3971174
                                                                                                    |                                                                                                   
TGGCGCGCTATCGTGACCTGATCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGG                                                                                                     <  SRR4302518.4866038/101‑1 (MQ=60)
TGGCGCACTATCGTGACCTGATCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCNNN                                                                                                     >  SRR4302518.11583266/1‑101 (MQ=60)
 TGCGCACTATCGTGACCTGATCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTNNN                                                                                                    >  SRR4302518.11907927/1‑101 (MQ=60)
     NNNTATCGTGACCTGATCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCC                                                                                                <  SRR4302518.8674359/101‑1 (MQ=60)
     NNNTATCGTGACCTGATCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCC                                                                                                <  SRR4302518.10898970/101‑1 (MQ=60)
     CACTATCGTGACCTGATCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCC                                                                                                >  SRR4302518.3134910/1‑101 (MQ=60)
     CACTATCGTGACCTGATCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCC                                                                                                >  SRR4302518.3583661/1‑101 (MQ=60)
      CCTATCGTGACCTGATCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCG                                                                                               <  SRR4302518.5462185/101‑1 (MQ=60)
      ACTATCGTGACCTGATCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCG                                                                                               >  SRR4302518.3019753/1‑101 (MQ=60)
        TATCGTGTCCTGATCACCTTTGTCGCTGACCGTCCGGGGAATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCA                                                                                             <  SRR4302518.695314/101‑1 (MQ=60)
        TATCGTGACCTGATCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCA                                                                                             >  SRR4302518.2965682/1‑101 (MQ=60)
         ATCGTGACCTGATCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAG                                                                                            >  SRR4302518.3834606/1‑101 (MQ=60)
         ATCGTGACCTGATCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAG                                                                                            <  SRR4302518.2535823/101‑1 (MQ=60)
           NNNGACCTGATCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGA                                                                                          <  SRR4302518.12332740/101‑1 (MQ=60)
           CGTGACCTGATCACCTTTGTCGCTGACCGTTCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGA                                                                                          >  SRR4302518.740158/1‑101 (MQ=60)
            GTGACCTGATCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAA                                                                                         >  SRR4302518.2788255/1‑101 (MQ=60)
             NNNCCTGATCGCCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAA                                                                                        <  SRR4302518.12948021/101‑1 (MQ=60)
              GACCTGATCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGANNN                                                                                       >  SRR4302518.10014454/1‑101 (MQ=60)
               NNNTGATCACCGTTGTCGCTGGCCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACC                                                                                      <  SRR4302518.9939505/101‑1 (MQ=60)
               ACCTGATCACCTTTGGCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACC                                                                                      >  SRR4302518.4528465/1‑101 (MQ=60)
               AACTGATCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAANNN                                                                                      >  SRR4302518.12449319/1‑101 (MQ=60)
                CCTGATCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAACATTGCCCGTGACCTTGGCTGGCTGCCGCAGGAAACCT                                                                                     <  SRR4302518.5530171/101‑1 (MQ=60)
                CCCGATCACCGTTGTCGCTGATAGTCCGGCGTATGCTCAGAGTTATGCCATTGATGCTTCGAAAATTGCCCGTGATCTTGGATGGCTGCCTCAGGAAACCT                                                                                     <  SRR4302518.4745233/101‑1 (MQ=23)
                 NTGATCACCTTTGTCGCTGACCGTCCGGGGCATGATCGGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTN                                                                                    >  SRR4302518.1439952/1‑101 (MQ=60)
                 NNNATCACCTCTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTT                                                                                    <  SRR4302518.8652643/101‑1 (MQ=60)
                 CTGATCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTT                                                                                    >  SRR4302518.3591775/1‑101 (MQ=60)
                 CTGATCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTT                                                                                    >  SRR4302518.6672175/1‑101 (MQ=60)
                  TGATCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTT                                                                                   >  SRR4302518.498072/1‑101 (MQ=60)
                  TGATCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCNNN                                                                                   >  SRR4302518.8345492/1‑101 (MQ=60)
                  TGATCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTGGGCTGGCTGCCGCAGGAAACCTTT                                                                                   >  SRR4302518.2223800/1‑101 (MQ=60)
                   GATCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTG                                                                                  <  SRR4302518.3260755/101‑1 (MQ=60)
                   GATCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTNNN                                                                                  >  SRR4302518.11695057/1‑101 (MQ=60)
                   GATCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTNNN                                                                                  >  SRR4302518.13555056/1‑101 (MQ=60)
                    ATCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGGTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGA                                                                                 >  SRR4302518.1505946/1‑101 (MQ=60)
                      CACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGNNN                                                                               >  SRR4302518.8001851/1‑101 (MQ=60)
                      CACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAAACTTTGAAA                                                                               >  SRR4302518.4379614/1‑101 (MQ=60)
                      CACCATTGTCGCTGACCGCCCGGGGCATGATCTGCGTTACGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGNNN                                                                               >  SRR4302518.13195318/1‑101 (MQ=60)
                       ACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGANNN                                                                              >  SRR4302518.11719794/1‑101 (MQ=60)
                       ACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAG                                                                              >  SRR4302518.5325673/1‑101 (MQ=60)
                       ACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGGTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAACCCTTTGAAAG                                                                              >  SRR4302518.1576765/1‑101 (MQ=60)
                        NNNTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGT                                                                             <  SRR4302518.13984603/101‑1 (MQ=60)
                        CCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGT                                                                             <  SRR4302518.6698823/101‑1 (MQ=60)
                         CTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAANNN                                                                            >  SRR4302518.8750568/1‑101 (MQ=60)
                         CTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTG                                                                            >  SRR4302518.2347733/1‑101 (MQ=60)
                         CTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTG                                                                            >  SRR4302518.4753186/1‑101 (MQ=60)
                          TTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGA                                                                           >  SRR4302518.6544529/1‑101 (MQ=60)
                          TTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGNNN                                                                           >  SRR4302518.7677280/1‑101 (MQ=60)
                          NNNGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTCG                                                                           <  SRR4302518.8050653/101‑1 (MQ=60)
                           TTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGA                                                                          <  SRR4302518.6066019/101‑1 (MQ=60)
                           TTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGA                                                                          >  SRR4302518.5576225/1‑101 (MQ=60)
                           TTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGA                                                                          <  SRR4302518.3457833/101‑1 (MQ=60)
                            TGTCGCTGACCGTCCGGGGCATGATCTGCGGTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCCGGAAACCTTTGAAAGTGGAG                                                                         >  SRR4302518.5971784/1‑101 (MQ=60)
                            NNNCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAA                                                                         <  SRR4302518.10268951/101‑1 (MQ=60)
                            NNNCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAA                                                                         <  SRR4302518.12195210/101‑1 (MQ=60)
                            NNNCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAA                                                                         <  SRR4302518.9746345/101‑1 (MQ=60)
                             NNNGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAAT                                                                        <  SRR4302518.11981723/101‑1 (MQ=60)
                             GTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAAT                                                                        <  SRR4302518.5287985/101‑1 (MQ=60)
                             GTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAAT                                                                        <  SRR4302518.2258088/101‑1 (MQ=60)
                              TCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATG                                                                       <  SRR4302518.3669833/101‑1 (MQ=60)
                              TCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATG                                                                       <  SRR4302518.605737/101‑1 (MQ=60)
                              TCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGGGGANNN                                                                       >  SRR4302518.8293303/1‑101 (MQ=60)
                              NNNCTGACCGTCCGGGGCATGATCTGCTTTATGCCATTGATTCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATG                                                                       <  SRR4302518.13289227/101‑1 (MQ=60)
                              NNNCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATG                                                                       <  SRR4302518.10057788/101‑1 (MQ=60)
                               NNNTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGC                                                                      <  SRR4302518.9636837/101‑1 (MQ=60)
                               NNNTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGC                                                                      <  SRR4302518.7433102/101‑1 (MQ=60)
                               CTCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGTCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAANNN                                                                      >  SRR4302518.8175208/1‑101 (MQ=60)
                               CGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAANNN                                                                      >  SRR4302518.10071161/1‑101 (MQ=60)
                                NNNGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTGGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCG                                                                     <  SRR4302518.9352011/101‑1 (MQ=60)
                                NNNGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCG                                                                     <  SRR4302518.13549303/101‑1 (MQ=60)
                                GCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCG                                                                     <  SRR4302518.2375311/101‑1 (MQ=60)
                                GCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCG                                                                     <  SRR4302518.4972106/101‑1 (MQ=60)
                                GCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCG                                                                     <  SRR4302518.1485891/101‑1 (MQ=60)
                                 NNNACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGT                                                                    <  SRR4302518.10918503/101‑1 (MQ=60)
                                 CTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGT                                                                    <  SRR4302518.6281975/101‑1 (MQ=60)
                                  TGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGTAATGCGTA                                                                   >  SRR4302518.4138641/1‑101 (MQ=60)
                                  TGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTA                                                                   <  SRR4302518.2036986/101‑1 (MQ=60)
                                  NNNCCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGCA                                                                   <  SRR4302518.11341952/101‑1 (MQ=60)
                                   GACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAA                                                                  >  SRR4302518.3556435/1‑101 (MQ=60)
                                   GACCGTACGGGGCATGATCAGAGATATGCGATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAA                                                                  <  SRR4302518.4245018/101‑1 (MQ=60)
                                    ACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTNNN                                                                 >  SRR4302518.13993556/1‑101 (MQ=60)
                                     CCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAA                                                                <  SRR4302518.3180327/101‑1 (MQ=60)
                                     CCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTCCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAA                                                                >  SRR4302518.2727726/1‑101 (MQ=60)
                                      CGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGGAAGTGGAATGAGTAATAC                                                               >  SRR4302518.1653980/1‑101 (MQ=60)
                                      CGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAAC                                                               >  SRR4302518.726181/1‑101 (MQ=60)
                                       NNNCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACG                                                              <  SRR4302518.12088714/101‑1 (MQ=60)
                                       GTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACG                                                              >  SRR4302518.5758737/1‑101 (MQ=60)
                                       GTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACG                                                              <  SRR4302518.2368329/101‑1 (MQ=60)
                                        TCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGG                                                             >  SRR4302518.7112785/1‑101 (MQ=60)
                                         CCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACNNN                                                            >  SRR4302518.12597730/1‑101 (MQ=60)
                                          CGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAACCCTTTGAAAGTGGAATGCGTAAAACGGTT                                                           <  SRR4302518.4876882/101‑1 (MQ=60)
                                          CGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGNNN                                                           >  SRR4302518.9909970/1‑101 (MQ=60)
                                           NNNGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGACCTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTC                                                          <  SRR4302518.8882077/101‑1 (MQ=60)
                                           GGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTC                                                          <  SRR4302518.2774460/101‑1 (MQ=60)
                                           GGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTC                                                          >  SRR4302518.5745876/1‑101 (MQ=60)
                                             GGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGGGGAATGCGTAAAACGGgtnnn                                                        >  SRR4302518.8528146/1‑96 (MQ=60)
                                              GCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCTCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCNNN                                                       >  SRR4302518.10616093/1‑101 (MQ=60)
                                               CATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACGTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCANNN                                                      >  SRR4302518.12676273/1‑101 (MQ=60)
                                                ATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGG                                                     >  SRR4302518.3697557/1‑101 (MQ=60)
                                                ATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGG                                                     >  SRR4302518.1797490/1‑101 (MQ=60)
                                                ATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGNNN                                                     >  SRR4302518.12898490/1‑101 (MQ=60)
                                                 TGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGT                                                    >  SRR4302518.4297198/1‑101 (MQ=60)
                                                 NNNTCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGT                                                    <  SRR4302518.12378634/101‑1 (MQ=60)
                                                  NNNCTGCGTTATGCCATTGATGCTTCGAAAATTGCACGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGCA                                                   <  SRR4302518.10757759/101‑1 (MQ=60)
                                                  GATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGNNN                                                   >  SRR4302518.12582078/1‑101 (MQ=60)
                                                  GATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGNNN                                                   >  SRR4302518.8971183/1‑101 (MQ=60)
                                                  GATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGNNN                                                   >  SRR4302518.9567922/1‑101 (MQ=60)
                                                  GATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTA                                                   >  SRR4302518.6728240/1‑101 (MQ=60)
                                                    TCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGACGCAGGAAACCTTTGAAAGGGGGATGCGTAAAACGGTTCAGTGGTATC                                                 >  SRR4302518.1640569/1‑101 (MQ=60)
                                                     CTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCT                                                >  SRR4302518.3425774/1‑101 (MQ=60)
                                                     CTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCT                                                <  SRR4302518.5252895/101‑1 (MQ=60)
                                                      TGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGTAAGTGGAATGCGTAAAACGGTTCAGTGttttnnn                                               >  SRR4302518.7669852/1‑94 (MQ=60)
                                                      TACGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTAAGTGGTATCTG                                               >  SRR4302518.3757694/1‑101 (MQ=60)
                                                      NNNGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTG                                               <  SRR4302518.12455815/101‑1 (MQ=60)
                                                      NNNGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTG                                               <  SRR4302518.8915934/101‑1 (MQ=60)
                                                       NNNTTATGCGATTAATGCTTCAAAAATTGCCCGTGAACTTGGCTGACTGCCGCAGGAAACCTTTGAAAGTGAAATGCGTAAAACGGTTCAGTGGTATCTGG                                              <  SRR4302518.8110930/101‑1 (MQ=60)
                                                       NNNTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGG                                              <  SRR4302518.14578766/101‑1 (MQ=60)
                                                        NNNTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGC                                             <  SRR4302518.8184271/101‑1 (MQ=60)
                                                        CGTTATGCCATTGATGCTTCGAAAATTGCCCGTGACCTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGC                                             <  SRR4302518.2094795/101‑1 (MQ=60)
                                                        CGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTNNN                                             >  SRR4302518.8320946/1‑101 (MQ=60)
                                                        CGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGC                                             >  SRR4302518.7217031/1‑101 (MQ=60)
                                                         GTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGTT                                            <  SRR4302518.3623789/101‑1 (MQ=60)
                                                         GTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCT                                            <  SRR4302518.2110579/101‑1 (MQ=60)
                                                         GTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCT                                            >  SRR4302518.283398/1‑101 (MQ=60)
                                                         GTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCT                                            <  SRR4302518.2238009/101‑1 (MQ=60)
                                                         GGTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAAGGCGTAAAACGGTTCAGTGGTATCTGNNN                                            >  SRR4302518.11066227/1‑101 (MQ=60)
                                                          NNNTGCCCTTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGCAAAACGGTTCAGTGGTATCTGGCTA                                           <  SRR4302518.9683741/101‑1 (MQ=60)
                                                          NNNTGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTA                                           <  SRR4302518.14039584/101‑1 (MQ=60)
                                                           nnngtCATTGATGCTTCTAAAATTTCCCGTGAACTTGGCTGTCTGCCTCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAA                                          <  SRR4302518.8369627/96‑1 (MQ=60)
                                                             TGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGACTANNN                                        >  SRR4302518.13481793/1‑101 (MQ=60)
                                                             NNNCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATG                                        <  SRR4302518.9580293/101‑1 (MQ=60)
                                                              GCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGA                                       >  SRR4302518.6898453/1‑101 (MQ=60)
                                                              GCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAANNN                                       >  SRR4302518.12899474/1‑101 (MQ=60)
                                                              GCCATTGATGCTACGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGA                                       <  SRR4302518.7268037/101‑1 (MQ=60)
                                                               nnnnnnnnncnTCGAAAATTGCNCNTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAA                                      <  SRR4302518.14826048/90‑1 (MQ=60)
                                                               NNNTTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGCA                                      <  SRR4302518.8189478/101‑1 (MQ=60)
                                                               NNNTTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAA                                      <  SRR4302518.13782518/101‑1 (MQ=60)
                                                               NNNTTGATGCTTCGAAAACTGCCCGTGAACTTGGCTGTCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAA                                      <  SRR4302518.8829043/101‑1 (MQ=60)
                                                               CCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAA                                      <  SRR4302518.2572834/101‑1 (MQ=60)
                                                               CCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAA                                      >  SRR4302518.6295645/1‑101 (MQ=60)
                                                                CATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGNNN                                     >  SRR4302518.14240672/1‑101 (MQ=60)
                                                                CATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAA                                     >  SRR4302518.3547990/1‑101 (MQ=60)
                                                                 TTTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGANNN                                    >  SRR4302518.11837710/1‑101 (MQ=60)
                                                                 NNNGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAG                                    <  SRR4302518.10203498/101‑1 (MQ=60)
                                                                 ATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGTAAGTGGAATGCGTAAAACGGTTCAGTGGTTTCTGGTTAATGAAAG                                    >  SRR4302518.3176601/1‑101 (MQ=60)
                                                                 ATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCGGGCTAATGANNN                                    >  SRR4302518.14859764/1‑101 (MQ=60)
                                                                  TTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGC                                   <  SRR4302518.2531163/101‑1 (MQ=60)
                                                                   TGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAATCCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCT                                  <  SRR4302518.4882839/101‑1 (MQ=60)
                                                                   TGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTCTCTGGCGAATGAAANNN                                  >  SRR4302518.9525874/1‑101 (MQ=60)
                                                                   TGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCT                                  <  SRR4302518.1977259/101‑1 (MQ=60)
                                                                    GATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTG                                 >  SRR4302518.6690690/1‑101 (MQ=60)
                                                                    GATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGGATCTGGCTAATGAAAGCTG                                 >  SRR4302518.6331961/1‑101 (MQ=60)
                                                                    GATGATTCGAAAATGGCCCGTGATCTTGGCTGGCTGACGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTG                                 <  SRR4302518.5053655/101‑1 (MQ=60)
                                                                     NNNCTTCGTAAATTGCCCGTGAACTTGTCTGGCTGCCGCATGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTCG                                <  SRR4302518.11313718/101‑1 (MQ=60)
                                                                     ATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTTCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCCGGCTAATGAAAGCNNN                                >  SRR4302518.10530251/1‑101 (MQ=60)
                                                                      TGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGG                               >  SRR4302518.6170047/1‑101 (MQ=60)
                                                                       NNNTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTG                              <  SRR4302518.8455725/101‑1 (MQ=60)
                                                                       GCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGNNN                              >  SRR4302518.10130524/1‑101 (MQ=60)
                                                                        CTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGG                             >  SRR4302518.6528439/1‑101 (MQ=60)
                                                                        CTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGGGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGNNN                             >  SRR4302518.10346874/1‑101 (MQ=60)
                                                                         TTCGGAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGGATCTGGCTAATGAAAacagggnnn                            >  SRR4302518.11131971/1‑92 (MQ=60)
                                                                         TTCGAAAATTGCCCGTAAAATTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGA                            <  SRR4302518.1424584/101‑1 (MQ=60)
                                                                          TCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAA                           <  SRR4302518.229751/101‑1 (MQ=60)
                                                                          TCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAA                           >  SRR4302518.779322/1‑101 (MQ=60)
                                                                          TCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAA                           >  SRR4302518.2749679/1‑101 (MQ=60)
                                                                           CGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAG                          <  SRR4302518.5585835/101‑1 (MQ=60)
                                                                            GAAAATTGCCCGTGAACTTGGCTTGCTGCCGCAGGAAACCTTTGAAAGTGGAATTCGTAAAACGGTTCAGTGGTATCTGGCTAATAAAAGCTGGTGGAAGC                         >  SRR4302518.7069845/1‑101 (MQ=60)
                                                                             gatcTTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCA                        <  SRR4302518.5839816/97‑1 (MQ=60)
                                                                             AAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGgannn                        >  SRR4302518.13813644/1‑96 (MQ=60)
                                                                             AAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCA                        <  SRR4302518.3726089/101‑1 (MQ=60)
                                                                              AAATTGCCCGTGAACTTGGATGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAG                       <  SRR4302518.4593959/101‑1 (MQ=60)
                                                                               NNNTGCCCGTGAACTTGGCTGTCTGCCGCAGGAACCCTTTGAAAGTGGAATGCGTAAAACGGTGCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAAG                      <  SRR4302518.10356155/101‑1 (MQ=60)
                                                                                ATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCANNN                     >  SRR4302518.7508155/1‑101 (MQ=60)
                                                                                 NNNCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTG                    <  SRR4302518.9069084/101‑1 (MQ=60)
                                                                                  NNNCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGC                   <  SRR4302518.14655236/101‑1 (MQ=60)
                                                                                   GCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCA                  >  SRR4302518.3142363/1‑101 (MQ=60)
                                                                                    NNNGTGAACTTGGCTGGCTGCCGCAGGAAGCCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAG                 <  SRR4302518.13811075/101‑1 (MQ=60)
                                                                                    CCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAG                 <  SRR4302518.7238163/101‑1 (MQ=60)
                                                                                    CCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAG                 <  SRR4302518.856305/101‑1 (MQ=60)
                                                                                     CCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCNNN                >  SRR4302518.10398813/1‑101 (MQ=60)
                                                                                     CCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCNNN                >  SRR4302518.12430014/1‑101 (MQ=60)
                                                                                     CCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGG                <  SRR4302518.443575/101‑1 (MQ=60)
                                                                                       GTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGAC              <  SRR4302518.680297/101‑1 (MQ=60)
                                                                                       GTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGAC              <  SRR4302518.3344198/101‑1 (MQ=60)
                                                                                       GTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGGCCAGGAC              >  SRR4302518.6858913/1‑101 (MQ=60)
                                                                                       GAGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGNNN              >  SRR4302518.7915552/1‑101 (MQ=60)
                                                                                        TGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGNNN             >  SRR4302518.11276975/1‑101 (MQ=60)
                                                                                        TGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGGGGAAGCAGGTGCAGGNNN             >  SRR4302518.11751290/1‑101 (MQ=60)
                                                                                         NNNCTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGG            <  SRR4302518.9178546/101‑1 (MQ=60)
                                                                                         GAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGGGGAAGCAGGTGCGGGACGG            >  SRR4302518.5751697/1‑101 (MQ=60)
                                                                                          AACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGC           >  SRR4302518.7238915/1‑101 (MQ=60)
                                                                                          AACTTGGCTGGCTGCCGCAGGAAACCCTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACNNN           >  SRR4302518.12943340/1‑101 (MQ=60)
                                                                                           ACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCA          <  SRR4302518.6709537/101‑1 (MQ=60)
                                                                                           ACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGGATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGNNN          >  SRR4302518.14676136/1‑101 (MQ=60)
                                                                                            CTTGTCTGCCTGCCTCTGGAATCCTTTGAAAGTGTAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAG         <  SRR4302518.3918831/101‑1 (MQ=60)
                                                                                            CTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGNNN         >  SRR4302518.13075088/1‑101 (MQ=60)
                                                                                            CTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAG         <  SRR4302518.469221/101‑1 (MQ=60)
                                                                                            CTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGGATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGNNN         >  SRR4302518.14304333/1‑101 (MQ=60)
                                                                                             TTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGC        >  SRR4302518.5149030/1‑101 (MQ=60)
                                                                                               GGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTA      >  SRR4302518.1532644/1‑101 (MQ=60)
                                                                                                   NNNTGCCGCAGGAATCCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAG  <  SRR4302518.14206430/101‑1 (MQ=60)
                                                                                                    |                                                                                                   
TGGCGCACTATCGTGACCTGATCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGCGTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAG  >  NC_000913/3970975‑3971174

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑