Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1624	250.6	12620048	98.0%	12367647	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	996,684	A→G	S18P (TCC→CCC)	ycbP ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	996,684	0	A	G	100.0%	598.1 / NA	187	S18P (TCC→CCC)	ycbP	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (72/115); total (72/115)

Only 100 of 188 total aligned reads displayed.

GTAGATCTTCCGGGGCGAAGTTTTGCAGATTCCAGTGATAAACCTCTACATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGAGCTGGAGCGAGAAGGAAAGCGAGGACTACCCGCCAGGGTGATGACACGCATACTCTCTCCTTATAACCAATTGTTCTTTTTTTGTTAAC  >  NC_000913/996594‑996773

gTAGATCTTCCGGGGCGAAGTTTTGCAGATTCCAGTGATAAACCTCTACATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGGGc                                                                                         <  1:2420411/93‑1 (MQ=255)

gTAGATCTTCCGGGGCGAAGTTTTGCAGATTCCAGTGATAAACCTCTACATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGGGc                                                                                         <  1:3918620/93‑1 (MQ=255)

 tAGATCTTCCGGGGCGAAGTTTTGCAGATTCCAGTGATAAACCTCTACATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGGGCt                                                                                        >  2:409828/1‑93 (MQ=255)

 tAGATCTTCCGGGGCGAAGTTTTGCAGATTCCAGTGATAAACCTCTACATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGGGCt                                                                                        <  2:2113176/93‑1 (MQ=255)

   gATCTTCCGGGGCGAAGTTTTGCAGATTCCAGTGATAAACCTCTACATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGGGCTgg                                                                                      <  2:2494797/93‑1 (MQ=255)

    aTCTTCCGGGGCGAAGTTTTGCAGATTCCAGTGATAAACCTCTACATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGGGCTGGa                                                                                     <  1:3993913/93‑1 (MQ=255)

    aTCTTCCGGGGCGAAGTTTTGCAGATTCCAGTGATAAACCTCTACATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGGGCTGGa                                                                                     <  2:2896177/93‑1 (MQ=255)

    aTCTTCCGGGGCGAAGTTTTGCAGATTCCAGTGATAAACCTCTACATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGGGCTGGa                                                                                     >  2:2094331/1‑93 (MQ=255)

     tCTTCCGGGGCGAAGTTTTGCAGATTCCAGTGATAAACCTCTACATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGGGCTGGAg                                                                                    >  1:3105401/1‑93 (MQ=255)

      cTTCCGGGGCGAAGTTTTGCAGATTCCAGTGATAAACCTCTACATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGGGCTGGAGc                                                                                   <  2:4990621/93‑1 (MQ=255)

       ttCCGGGGCGAAGTTTTGCAGATTCCAGTGATAAACCTCTACATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGGGCTGGAGCg                                                                                  >  1:3094013/1‑93 (MQ=255)

         ccGGGGCGAAGTTTTGCAGATTCCAGTGATAAACCTCTACATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGGGCTGGAGCgag                                                                                <  1:1696017/93‑1 (MQ=255)

          cGGGGCGAAGTTTTGCAGATTCCAGTGATAAACCTCTACATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGGGCTGGAGCgaga                                                                               >  1:1649997/1‑93 (MQ=255)

           ggggCGAAGTTTTGCAGATTCCAGTGATAAACCTCTACATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGGGCTGGAGCGAGaa                                                                              <  2:796481/93‑1 (MQ=255)

           ggggCGAAGTTTTGCAGATTCCAGTGATAAACCTCTACATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGGGCTGGAGCGAGaa                                                                              <  1:3219727/93‑1 (MQ=255)

           ggggCGAAGTTTTGCAGATTCCAGTGATAAACCTCTACATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGGGCTGGAGCGAGaa                                                                              <  1:641975/93‑1 (MQ=255)

             ggCGAAGTTTTGCAGATTCCAGTGATAAACCTCTACATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGGGCTGGAGCGAGAAgg                                                                            >  1:1041993/1‑93 (MQ=255)

               cGAAGTTTTGCAGATTCCAGTGATAAACCTCTACATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGGGCTGGAGCGAGAAGGaa                                                                          <  2:1410582/93‑1 (MQ=255)

                 aaGTTTTGCAGATTCCAGTGATAAACCTCTACATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGGGCTGGAGCGAGAAGGAAAg                                                                        <  1:4508446/93‑1 (MQ=255)

                   gTTTTGCAGATTCCAGTGATAAACCTCTACATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGGGCTGGAGCGAGAAGGAAAGCg                                                                      >  1:3472742/1‑93 (MQ=255)

                   gTTTTGCAGATTCCAGTGATAAACCTCTACATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGGGCTGGAGCGAGAAGGAAAGCg                                                                      >  2:1588622/1‑93 (MQ=255)

                    ttttGCAGATTCCAGTGATAAACCTCTACATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGGGCTGGAGCGAGAAGGAAAGCGa                                                                     >  1:3405096/1‑93 (MQ=255)

                    gtttGCAGATTGCAGTGATAAGCCTCTACATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGGGCTGGAGCGAGAAGGAAAGCGa                                                                     <  1:1747038/92‑1 (MQ=255)

                     tttGCAGATTCCAGTGATAAACCTCTACATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGGGCTGGAGCGAGAAGGAAAGCGAg                                                                    <  1:3029198/93‑1 (MQ=255)

                     tttGCAGATTCCAGTGATAAACCTCTACATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGGGCTGGAGCGAGAAGGAAAGCGAg                                                                    <  1:2509381/93‑1 (MQ=255)

                        gCAGATTCCAGTGATAAACCTCTACATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGGGCTGGAGCGAGAAGGAAAGCGAGGAc                                                                 <  1:5827394/93‑1 (MQ=255)

                         cAGATTCCAGTGATAAACCTCTACATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGGGCTGGAGCGAGAAGGAAAGCGAGGACt                                                                >  2:4451710/1‑93 (MQ=255)

                          aGATTCCAGTGATAAACCTCTACATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGGGCTGGAGCGAGAAGGAAAGCGAGGACTa                                                               <  1:1100330/93‑1 (MQ=255)

                           gATTCCAGTGATAAACCTCTACATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGGGCTGGAGCGAGAAGGAAAGCGAGGACTAc                                                              <  1:4242118/93‑1 (MQ=255)

                           gATTCCAGTGATAAACCTCTACATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGGGCTGGAGCGAGAAGGAAAGCGAGGACTAc                                                              >  1:692096/1‑93 (MQ=255)

                            aTTCCAGTGATAAACCTCTACATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGGGCTGGAGCGAGAAGGAAAGCGAGGACTAcc                                                             <  2:5734104/93‑1 (MQ=255)

                             ttCCAGTGATAAACCTCTACATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGGGCTGGAGCGAGAAGGAAAGCGAGGACTAccc                                                            <  1:6048238/93‑1 (MQ=255)

                              tCCAGTGATAAACCTCTACATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGGGCTGGAGCGAGAAGGAAAGCGAGGACTACCCg                                                           >  2:4859135/1‑93 (MQ=255)

                                cAGTGATAAACCTCTACATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGGGCTGGAGCGAGAAGGAAAGCGAGGACTACCCGcc                                                         >  1:2821210/1‑93 (MQ=255)

                                 aGTGATAAACCTCTACATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGGGCTGGAGCGAGAAGGAAAGCGAGGACTACCCGCCa                                                        <  2:2020366/93‑1 (MQ=255)

                                  gTGATAAACCTCTACATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGGGCTGGAGCGAGAAGGAAAGCGAGGACTACCCGCCAg                                                       <  2:4090774/93‑1 (MQ=255)

                                  gTGATAAACCTCTACATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGGGCTGGAGCGAGAAGGAAAGCGAGGACTACCCGCCAg                                                       <  1:1455414/93‑1 (MQ=255)

                                  gTGATAAACCTCTACATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGGGCTGGAGCGAGAAGGAAAGCGAGGACTACCCGCCAg                                                       >  1:4950981/1‑93 (MQ=255)

                                    gATAAACCTCTACATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGGGCTGGAGCGAGAAGGAAAGCGAGGACTACCCGCCAggg                                                     <  1:2633783/93‑1 (MQ=255)

                                     aTAAACCTCTACATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGGGCTGGAGCGAGAAGGAAAGCGAGGACTACCCGCCAGGGt                                                    >  2:5685293/1‑93 (MQ=255)

                                     aTAAACCTCTACATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGGGCTGGAGCGAGAAGGAAAGCGAGGACTACCCGCCAGGGt                                                    <  2:1059518/93‑1 (MQ=255)

                                     aTAAACCTCTACATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGGGCTGGAGCGAGAAGGAAAGCGAGGACTAACCGCCAGGGt                                                    >  2:2399906/1‑93 (MQ=255)

                                       aaaCCTCTCCATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGGGCTGGAGCGAGAAGGAAAGCGAGGACTACCCGCCAGGGtga                                                  <  1:479361/93‑1 (MQ=255)

                                       aaaCCTCTACATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGGGCTGGAGCGAGAAGGAAAGCGAGGACTACCCGCCCGGGtga                                                  <  2:5239232/93‑1 (MQ=255)

                                       aaaCCTCTACATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGGGCTGGAGCGAGAAGGAAAGCGAGGACTACCCGCCAGGGtga                                                  <  1:892206/93‑1 (MQ=255)

                                       aaaCCTCTACATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGGGCTGGAGCGAGAAGGAAAGCGAGGACTACCCGCCAGGGtga                                                  >  1:374780/1‑93 (MQ=255)

                                        aaCCTCTACATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGGGCTGGAGCGAGAAGGAAAGCGAGGACTACCCGCCAGGGtgat                                                 <  1:3712226/93‑1 (MQ=255)

                                         aCCTCTACATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGGGCTGGAGCGAGAAGGAAAGCGAGGACTACCCGCCAGGGtgatg                                                >  2:2713411/1‑93 (MQ=255)

                                         aCCTCTACATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGGGCTGGAGCGAGAAGGAAAGCGAGGACTACCCGCCAGGGtgatg                                                <  1:2537518/93‑1 (MQ=255)

                                           ctctACATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGGGCTGGAGCGAGAAGGAAAGCGAGGACTACCCGCCAGGGTGATGac                                              <  1:5765128/93‑1 (MQ=255)

                                            tctACATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGGGCTGGAGCGAGAAGGAAAGCGAGGACTACCCGCCAGGGTGATGaca                                             >  2:3545317/1‑93 (MQ=255)

                                             ctACATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGGGCTGGAGCGAGAAGGAAAGCGAGGACTACCCGCCAGGGTGATGacac                                            >  2:919371/1‑93 (MQ=255)

                                                cATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGGGCTGGAGCGAGAAGGAAAGCGAGGACTACCCGCCAGGGTGATGACACGCa                                         >  2:4251170/1‑93 (MQ=255)

                                                cATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGGGCTGGAGCGAGAAGGAAAGCGAGGACTACCCGCCAGGGTGATGACACGCa                                         <  1:3359682/93‑1 (MQ=255)

                                                 aTCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGGGCTGGAGCGAGAAGGAAAGCGAGGACTACCCGCCAGGGTGATGACACGCAt                                        >  1:5456092/1‑93 (MQ=255)

                                                  tCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGGGCTGGAGCGAGAAGGAAAGCGAGGACTACCCGCCAGGGTGATGACACGCATa                                       <  1:91264/93‑1 (MQ=255)

                                                  tCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGGGCTGGAGCGAGAAGGAAAGCGAGGACTACCCGCCAGGGTGATGACACGCATa                                       <  2:3386359/93‑1 (MQ=255)

                                                  tCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGGGCTGGAGCGAGAAGGAAAGCGAGGACTACCCGCCAGGGTGATGACACGCATa                                       >  2:2075872/1‑93 (MQ=255)

                                                   ccAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGGGCTGGAGCGTGAAGGAAAGCGAGGACTACCCGCCAGGGTGATGACACGCATAc                                      <  1:5586956/93‑1 (MQ=255)

                                                   ccAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGGGCTGGAGCGAGAAGGAAAGCGAGGACTACCCGCCAGGGTGATGACACGCATAc                                      >  2:3134493/1‑93 (MQ=255)

                                                   ccAGCCCATTTAGTCTTCCCCGCGCATATTCCAGCAAGGGTCTGGACCGAGAAGAAAAGCGAGGCCTACCCGCCAGGGTGATGACACGCATAc                                      <  2:2261351/93‑1 (MQ=255)

                                                      ggCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGGGCTGGAGCGAGAAGGAAAGCGAGGACTACCCGCCAGGGTGATGACACGCATActct                                   <  2:4773867/93‑1 (MQ=255)

                                                         cATTTAGTTTTTCCCGCGCATATTCCAGCAAGGGGCTGGAGCGAGAAGGAAAGCGAGGACTACCCGCCAGGGTGATGACACGCATACtctctc                                <  1:2074435/93‑1 (MQ=255)

                                                          aTTTAGTTTTTCCCGCGCATATTCCAGCAAGGGGCTGGAGCGAGAAGGAAAGCGAGGACTACCCGCCAGGGTGATGACACGCATACTCTCTcc                               <  1:874278/93‑1 (MQ=255)

                                                          aTTTAGTTTTTCCCGCGCATATTCCAGCAAGGGGCTGGAGCGAGAAGGAAAGCGAGGACTACCCGCCAGGGTGATGACACGCATACTCTCTcc                               <  2:3727588/93‑1 (MQ=255)

                                                           tttAGTTTTTCCCGCGCATATTCCAGCAAGGGGCTGGAGCGAGAAGGAAAGCGAGGACTACCCGCCAGGGTGATGACACGCATACTCTCTCCt                              <  1:2218492/93‑1 (MQ=255)

                                                           tttAGTTTTTCCCGCGCATATTCCAGCAAGGGGCTGGAGCGAGAAGGAAAGCGAGGACTACCCGCCAGGGTGATGACACGCATACTCTCTCCt                              >  1:5195837/1‑93 (MQ=255)

                                                           tttAGTTTTTCCCGCGCATATTCCAGCAAGGGGCTGGAGCGAGAAGGAAAGCGAGGACTACCCGCCAGGGTGATGACACGCATACTCTCTCCt                              >  2:889609/1‑93 (MQ=255)

                                                            ttAGTTTTCCCCGCGCATATTCCAGCAAGGGGCTGGAGCGAGAAGGAAAGCGAGGACTACCCGCCAGGGTGATGACACGCATACTCTCTCCtt                             <  2:4300353/93‑1 (MQ=255)

                                                             tAGTTTTTCCCGCGCATATTCCAGCAAGGGGCTGGAGCGAGAAGGAAAGCGAGGACTACCCGCCAGGGTGATGACACGCATACTCTCTCCTta                            <  1:3082023/93‑1 (MQ=255)

                                                             tAGTTTTTCCCGCGCATATTCCAGCAAGGGGCTGGAGCGAGAAGGAAAGCGAGGACTACCCGCCAGGGTGATGACACGCATACTCTCTCCTta                            <  2:562227/93‑1 (MQ=255)

                                                             tAGTTTTTCCCGCGCATATTCCAGCAAGGGGCTGGAGCGAGAAGGAAAGCGAGGACTACCCGCCAGGGTGATGACACGCATACTCTCTCCTta                            <  2:6086561/93‑1 (MQ=255)

                                                             tAGTTTTTCCCGCGCATATTCCAGCAAGGGGCTGGAGCGAGAAGGAAAGCGAGGACTACCCGCCAGGGTGATGACACGCATACTCTCTCCTta                            <  2:3248930/93‑1 (MQ=255)

                                                              aGTTTTTCCCGCGCATATTCCAGCAAGGGGCTGGAGCGAGAAGGAAAGCGAGGACTCCCCGCCAGGGTGATGACACGCATACTCTCTCCTtat                           <  1:1186698/93‑1 (MQ=255)

                                                              aGTTTTTCCCGCGCATATTCCAGCAAGGGGCTGGAGCGAGAAGGAAAGCGAGGACTACCCGCCAGGGTGATGACACGCATACTCTCTCCTtat                           <  1:6006362/93‑1 (MQ=255)

                                                               gTTTTTCCCGCGCATATTCCAGCAAGGGGCTGGAGCGAGAAGGAAAGCGAGGACTACCCGCCAGGGTGATGACACGCATACTCTCTCCTtata                          <  2:4671495/93‑1 (MQ=255)

                                                               gTTTTTCCCGCGCATATTCCAGCAAGGGGCTGGAGCGAGAAGGAAAGCGAGGACTACCCGCCAGGGTGATGACACGCATACTCTCTCCTtata                          <  1:301646/93‑1 (MQ=255)

                                                                tttttCCCGCGCATATTCCAGCAAGGGGCTGGAGCGAGAAGGAAAGCGAGGACTACCCGCCAGGGTGATGACACGCATACTCTCTCCTTATaa                         >  2:1501387/1‑93 (MQ=255)

                                                                  tttCCCGCGCATATTCCAGCAAGGGGCTGGAGCGAGAAGGAAAGCGAGGACTACCCGCCAGGGTGATGACACGCATACTCTCTCCTTATAAcc                       <  2:4613907/93‑1 (MQ=255)

                                                                    tCCCGCGCATATTCCAGCAAGGGGCTGGAGCGAGAAGGAAAGCGAGGACTACCCGCCAGGGTGATGACACGCATACTCTCTCCTTATAACCaa                     <  1:3829187/93‑1 (MQ=255)

                                                                     cccGCGCATATTCCAGCAAGGGGCTGGAGCGAGAAGGAAAGCGAGGACTACCCGCCAGGGTGATGACACGCATACTCTCTCCTTATAACCAAt                    <  2:2608426/93‑1 (MQ=255)

                                                                     cccGCGCATATTCCAGCAAGGGGCTGGAGCGAGAAGGAAAGCGAGGACTACCCGCCAGGGTGATGACACGCATACTCTCTCCTTATAACCAAt                    >  2:5399299/1‑93 (MQ=255)

                                                                      ccGCGCATATTCCAGCAAGGGGCTGGAGCGAGAAGGAAAGCGAGGACTACCCGCCAGGGTGATGACACGCATACTCTCTCCTTATAACCAAtt                   >  2:3031268/1‑93 (MQ=255)

                                                                      ccGCGCATATTCCAGCAAGGGGCTGGAGCGAGAAGGAAAGCGAGGACTACCCGCCAGGGTGATGACACGCATACTCTCTCCTTATAACCAAtt                   <  1:1904375/93‑1 (MQ=255)

                                                                       cgcgCATATTCCAGCAAGGGGCTGGAGCGAGAAGGAAAGCGAGGACTACCCGCCAGGGTGATGACACGCATACTCTCTCCTTATAACCAATTg                  >  1:4600803/1‑93 (MQ=255)

                                                                       cgcgCATATTCCAGCAAGGGGCTGGAGCGAGAAGGAAAGCGAGGACTACCCGCCAGGGTGATGACACGCATACTCTCTCCTTATAACCAATTg                  >  2:629195/1‑93 (MQ=255)

                                                                       cgcgCATATTCCAGCAAGGGGCTGGAGCGAGAAGGAAAGCGAGCACTACCCGCCAGGGTGATGACACGCATACTCTCTCTTTATAACCAATTg                  <  2:4748668/93‑1 (MQ=255)

                                                                        gcgcATATTCCAGCAAGGGGCTGGAGCGAGAAGGAAAGCGAGGACTACCCGCCAGGGTGATGACACGCATACTCTCTCCTTATAACCAATTGt                 <  1:3965174/93‑1 (MQ=255)

                                                                          gcATATTCCAGCAAGGGGCTGGAGCGAGAAGGAAAGCGAGGACTACCCGCCAGGGTGATGACACGCATACTCTCTCCTTATAACCAATTGTTc               >  1:2896303/1‑93 (MQ=255)

                                                                           cATATTCCAGCAAGGGGCTGGAGCGAGAAGGAAAGCGAGGACTACCCGCCAGGGTGATGACACGCATACTCTCTCCTTATAACCAATTGTTCt              >  2:5446886/1‑93 (MQ=255)

                                                                            atatTCCAGCAAGGGGCTGGAGCGAGAAGGAAAGCGAGGACTACCCGCCAGGGTGATGACACGCATACTCTCTCCTTATAACCAATTGTTCtt             >  1:4869778/1‑93 (MQ=255)

                                                                            atatTCCAGCAAGGGGCTGGAGCGAGAAGGAAAGCGAGGACTACCCGCCAGGGTGATGACACGCATACTCTCTCCTTATAACCAATTGTTCtt             >  1:5903373/1‑93 (MQ=255)

                                                                            atacTCCAGCAAGGGGCTGGAGCGAGAAGGAAAGCGAGGACTACCCGCCAGGGTGATGACACGCATACTCTCTCCTTATAACCAATTGTTCtt             >  2:2718403/1‑93 (MQ=255)

                                                                             tatTCCAGCAAGGGGCTGGAGCGAGAAGGAAAGCGAGGACTACCCGCCAGGGTGATGACACGCATACTCTCTCCTTATAACCAATTGTTCttt            >  1:4800322/1‑93 (MQ=255)

                                                                              atTCCAGCAAGGGGCTGGAGCGAGAAGGAAAGCGAGGACTACCCGCCAGGGTGATGACACGCATACTCTCTCCTTATAACCAATTGTTCtttt           <  2:5993086/93‑1 (MQ=255)

                                                                                  cAGCAAGGGGCTGGAGCGAGAAGGAAAGCGAGGACTACCCGCCAGGGTGATGACACGCATACTCTCTCCTTATAACCAATTGTTCTTTTTTTg       <  2:2630463/93‑1 (MQ=255)

                                                                                  cAGCAAGGGGCTGGAGCGAGAAGGAAAGCGAGGACTACCCGCCAGGGTGATGACACGCATACTCTCTCCTTATAACCAATTGTTCTTTTTTTg       >  1:4059364/1‑93 (MQ=255)

                                                                                   aGCAAGGGGCTGGAGCGAGAAGGAAAGCGAGGACTACCCGCCAGGGTGATGACACGCATACTCTCTCCTTATAACCAATTGTTCTTTTTTTGt      <  2:6274882/93‑1 (MQ=255)

                                                                                     cAAGGGGCTGGAGCGAGAAGGAAAGCGAGGACTACCCGCCAGGGTGATGACACGCATACTCTCTCCTTATAACCAATTGTTCTTTTTTTGTTa    <  1:568756/93‑1 (MQ=255)

                                                                                       aGGGGCTGGAGCGAGAAGGAAAGCGAGGACTACCCGCCAGGGTGATGACACGCATACTCTCTCCTTATAACCAATTGTTCTTTTTTTGTTAAc  >  2:4783916/1‑93 (MQ=255)

GTAGATCTTCCGGGGCGAAGTTTTGCAGATTCCAGTGATAAACCTCTACATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGAGCTGGAGCGAGAAGGAAAGCGAGGACTACCCGCCAGGGTGATGACACGCATACTCTCTCCTTATAACCAATTGTTCTTTTTTTGTTAAC  >  NC_000913/996594‑996773

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

AAAGTAGATCTTCCGGGGCGAAGTTTTGCAGATTCCAGTGATAAACCTCTACATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGAGCTGGAGCGAGAAGGAAAGCGAGGACTACCCGCCAGGGTGATGACACGCATACTCTCTCCTTATAACCAATTGTTCTTTTTTTGTTAACAT  >  NC_000913/996591‑996775

AAAGTAGATCTTCCGGGGCGAAGTTTTGCAGATTCCAGTGATAAACCTCTACATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGG                                                                                             >  SRR4302533.2995971/1‑94 (MQ=60)

AAAGTAGATCTTCCGGGGCGAAGTTTTGCAGATTCCAGTGATAAACCTCTACATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGG                                                                                             <  SRR4302533.4002814/94‑1 (MQ=60)

AAAGTAGATCTTCCGGGGCGAAGTTTTGCAGATTCCAGTGATAAACCTCTACATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGG                                                                                             >  SRR4302533.1479541/1‑94 (MQ=60)

 AAGTAGATCTTCCGGGGCGAAGTTTTGCAGATTCCAGTGATAAACCTCTACATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGGG                                                                                            <  SRR4302533.5810321/94‑1 (MQ=60)

  AGTAGATCTTCCGGGGCGAAGTTTTGCAGATTCCAGTGATAAACCTCTACATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGGGC                                                                                           <  SRR4302533.830783/94‑1 (MQ=60)

   GTAGATCTTCCGGGGCGAAGTTTTGCAGATTCCAGTGATAAACCTCTACATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGGGCT                                                                                          <  SRR4302533.3942549/94‑1 (MQ=60)

   GTAGATCTTCCGGGGCGAAGTTTTGCAGATTCCAGTGATAAACCTCTACATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGGGCT                                                                                          <  SRR4302533.2435359/94‑1 (MQ=60)

    TAGATCTTCCGGGGCGAAGTTTTGCAGATTCCAGTGATAAACCTCTACATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGGGCTG                                                                                         <  SRR4302533.2328690/94‑1 (MQ=60)

       atttctCGGGGAGAAGTTTTGTAGGTTCCAGTGATAAACCTCTACAACCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGGGCTGGAG                                                                                      <  SRR4302533.1879431/88‑1 (MQ=60)

       ATCTTCCGGGGCGAAGTTTTGCAGATTCCAGTGATAAACCTCTACATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGGGCTGGAG                                                                                      <  SRR4302533.4018233/94‑1 (MQ=60)

       ATCTTCCGGGGCGAAGTTTTGCAGATTCCAGTGATAAACCTCTACATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGGGCTGGAG                                                                                      >  SRR4302533.3124490/1‑94 (MQ=60)

         CTTCCGGGGCGAAGTTTTGCAGATTCCAGTGATAAACCTCTACATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGGGCTGGAGCG                                                                                    >  SRR4302533.3113036/1‑94 (MQ=60)

         CTTCCGGGGCGAAGTTTTGCAGATTCCAGTGATAAACCTCTACATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGGGCTGGAGCG                                                                                    <  SRR4302533.4013654/94‑1 (MQ=60)

          TTCCGGGGCGAAGTTTTGCAGATTCCAGTGATAAACCTCTACATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGGGCTGGAGCGA                                                                                   >  SRR4302533.4746338/1‑94 (MQ=60)

            CCGGGGCGAAGTTTTGCAGATTCCAGTGATAAACCTCTACATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGGGCTGGAGCGAGA                                                                                 <  SRR4302533.1706289/94‑1 (MQ=60)

            CCGGGGCGAAGTTTTGCAGATTCCAGTGATAAACCTCTACATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGGGCTGGAGCGAGA                                                                                 >  SRR4302533.1659993/1‑94 (MQ=60)

              GGGGCGAAGTTTTGCAGATTCCAGTGATAAACCTCTACATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGGGCTGGAGCGAGAAG                                                                               <  SRR4302533.645749/94‑1 (MQ=60)

              GGGGCGAAGTTTTGCAGATTCCAGTGATAAACCTCTACATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGGGCTGGAGCGAGAAG                                                                               >  SRR4302533.1701501/1‑94 (MQ=60)

              GGGGCGAAGTTTTGCAGATTCCAGTGATAAACCTCTACATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGGGCTGGAGCGAGAAG                                                                               <  SRR4302533.3239579/94‑1 (MQ=60)

               GGGCGAAGTTTTGCAGATTCCAGTGATAAACCTCTACATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGGGCTGGAGCGAGAAGG                                                                              >  SRR4302533.1048121/1‑94 (MQ=60)

               GGGCGAAGTTTTGCAGATTCCAGTGATAAACCTCTACATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGGGCTGGAGCGAGAAGG                                                                              <  SRR4302533.154323/94‑1 (MQ=60)

                  CGAAGTTTTGCAGATTCCAGTGATAAACCTCTACATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGGGCTGGAGCGAGAAGGAAG                                                                           >  SRR4302533.3846829/1‑94 (MQ=60)

                    AAGTTTTGCAGATTCCAGTGATAAACCTCTACATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGGGCTGGAGCGAGAAGGAAAGC                                                                         <  SRR4302533.4535751/94‑1 (MQ=60)

                     AGTTTTGCAGATTCCAGTGATAAACCTCTACATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGGGCTGGAGCGAGAAGGAAAGCG                                                                        >  SRR4302533.3494172/1‑94 (MQ=60)

                     AGTTTTGCAGATTCCAGTGATAAACCTCTACATCCAGGCCATATAGTTTTTCCCGCGCATATTCCAGCAAGGGGCTGGAGCGAGAAGGAAAGCG                                                                        <  SRR4302533.3426242/94‑1 (MQ=60)

                      GTTTTGCAGATTCCAGTGATAAACCTCTACATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGGGCTGGAGCGAGAAGGAAAGCGA                                                                       >  SRR4302533.3426127/1‑94 (MQ=60)

                      GTTTTGCAGATTCCAGTGATAAACCTCTACATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGGGCTGGAGCGAGAAGGAAAGCGA                                                                       <  SRR4302533.3872109/94‑1 (MQ=60)

                       GTTTGCAGATTGCAGTGATAAGCCTCTACATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGGGCTGGAGCGAGAAGGAAAGCGAG                                                                      <  SRR4302533.1757670/94‑1 (MQ=60)

                        TTTGCAGATTCCAGTGATAAACCTCTACATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGGGCTGGAGCGAGAAGGAAAGCGAGG                                                                     <  SRR4302533.3047734/94‑1 (MQ=60)

                        TTTGCAGATTCCAGTGATAAACCTCTACATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGGGCTGGAGCGAGAAGGAAAGCGAGG                                                                     <  SRR4302533.2524841/94‑1 (MQ=60)

                           GCAGATTCCAGTGATAAACCTCTACATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGGGCTGGAGCGAGAAGGAAAGCGAGGACT                                                                  <  SRR4302533.5862604/94‑1 (MQ=60)

                             AGATTCCAGTGATAAACCTCTACATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGGGCTGGAGCGAGAAGGAAAGCGAGGACTAC                                                                <  SRR4302533.1106853/94‑1 (MQ=60)

                             AGATTCCAGTGATAAACCTCTACATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGGGCTGGAGCGAGAAGGAAAGCGAGGACTAC                                                                >  SRR4302533.696184/1‑94 (MQ=60)

                              GATTCCAGTGATAAACCTCTACATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGGGCTGGAGCGAGAAGGAAAGCGAGGACTACC                                                               <  SRR4302533.4267733/94‑1 (MQ=60)

                                TTCCAGTGATAAACCTCTACATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGGGCTGGAGCGAGAAGGAAAGCGAGGACTACCCG                                                             <  SRR4302533.6084884/94‑1 (MQ=60)

                                  CCAGTGATAAACCTCTACATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGGGCTGGAGCGAGAAGGAAAGCGAGGACTACCCGCC                                                           >  SRR4302533.2838573/1‑94 (MQ=60)

                                   CAGTGATAAACCTCTACATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGGGCTGGAGCGAGAAGGAAAGCGAGGACTACCCGCCA                                                          >  SRR4302533.2575042/1‑94 (MQ=60)

                                   CAGTGATAAACCTCTACATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGGGCTGGAGCGAGAAGGAAAGCGAGGACTACCCGCCA                                                          >  SRR4302533.6241233/1‑94 (MQ=60)

                                    AGTGATAAACCTCTACATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGGGCTGGAGCGAGAAGGAAAGCGAGGACTACCCGCCAG                                                         >  SRR4302533.4980965/1‑94 (MQ=60)

                                     GTGATAAACCTCTACATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGGGCTGGAGCGAGAAGGAAAGCGAGGACTACCCGCCAGG                                                        <  SRR4302533.1464188/94‑1 (MQ=60)

                                      TGATAAACCTCTACATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGGGCTGGAGCGAGAAGGAAAGCGAGGACTACCCGCCAGGG                                                       >  SRR4302533.3064730/1‑94 (MQ=60)

                                      TGATAAACCTCTACATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGGGCTGGAGCGAGAAGGAAAGCGAGGACTACCCGCCAGGG                                                       >  SRR4302533.906181/1‑94 (MQ=60)

                                       GATAAACCTCTACATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGGGCTGGAGCGAGAAGGAAAGCGAGGACTACCCGCCAGGGT                                                      <  SRR4302533.2649969/94‑1 (MQ=60)

                                        ATAAACCTCTACATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGGGCTGGAGCGAGAAGGAAAGCGAGGACTACCCGCCAGGGTG                                                     <  SRR4302533.1748966/94‑1 (MQ=60)

                                        ATAAACCTCTACATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGGGCTGGAGCGAGAAGGAAAGCGAGGACTACCCGCCAGGGTG                                                     <  SRR4302533.1248575/94‑1 (MQ=60)

                                         TAAACCTCTACATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGGGCTGGAGCGAGAAGGAAAGCGAGGACTACCCGCCAGGGTGA                                                    >  SRR4302533.377043/1‑94 (MQ=60)

                                          AAACCTCTCCATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGGGCTGGAGCGAGAAGGAAAGCGAGGACTACCCGCCAGGGTGAT                                                   <  SRR4302533.482225/94‑1 (MQ=60)

                                          AAACCTCTACATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGGGCTGGAGCGAGAAGGAAAGCGAGGACTACCCGCCAGGGTGAT                                                   >  SRR4302533.3263726/1‑94 (MQ=60)

                                          AAACCTCTACATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGGGCTGGAGCGAGAAGGAAAGCGAGGACTACCCGCCAGGGTGAT                                                   <  SRR4302533.897449/94‑1 (MQ=60)

                                           AACCTCTACATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGGGCTGGAGCGAGAAGGAAAGCGAGGACTACCCGCCAGGGTGATG                                                  <  SRR4302533.3735022/94‑1 (MQ=60)

                                            ACCTCTACATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGGGCTGGAGCGAGAAGGAAAGCGAGGACTACCCGCCAGGGTGATGA                                                 <  SRR4302533.2553141/94‑1 (MQ=60)

                                              CTCTACATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGGGCTGGAGCGAGAAGGAAAGCGAGGACTACCCGCCAGGGTGATGACA                                               <  SRR4302533.3107019/94‑1 (MQ=60)

                                              CTCTACATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGGGCTGGAGCGAGAAGGAAAGCGAGGACTACCCGCCAGGGTGATGACA                                               <  SRR4302533.4025936/94‑1 (MQ=60)

                                              CTCTACATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGGGCTGGAGCGAGAAGGAAAGCGAGGACTACCCGCCAGGGTGATGACA                                               <  SRR4302533.4466114/94‑1 (MQ=60)

                                              CTCTACATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGGGCTGGAGCGAGAAGGAAAGCGAGGACTACCCGCCAGGGTGATGACA                                               <  SRR4302533.5799919/94‑1 (MQ=60)

                                                   CATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGGGCTGGAGCGAGAAGGAAAGCGAGGACTACCCGCCAGGGTGATGACACGCAT                                          >  SRR4302533.5488963/1‑94 (MQ=60)

                                                   CATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGGGCTGGAGCGAGAAGGAAAGCGAGGACTACCCGCCAGGGTGATGACACGCAT                                          <  SRR4302533.3380439/94‑1 (MQ=60)

                                                     TCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGGGCTGGAGCGAGAAGGAAAGCGAGGACTACCCGCCAGGGTGATGACACGCATAC                                        <  SRR4302533.58132/94‑1 (MQ=60)

                                                     TCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGGGCTGGAGCGAGAAGGAAAGCGAGGACTACCCGCCAGGGTGATGACACGCATAC                                        <  SRR4302533.91843/94‑1 (MQ=60)

                                                      CCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGGGCTGGAGCGTGAAGGAAAGCGAGGACTACCCGCCAGGGTGATGACACGCATACT                                       <  SRR4302533.5620712/94‑1 (MQ=60)

                                                       CAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGGGCTGGAGCGAGAAGGAAAGCGAGGACTACCCGCCAGGGTGATGACACGCATACTC                                      >  SRR4302533.5759609/1‑94 (MQ=60)

                                                       CAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGGGCTGGAGCGAGAAGGAAAGCGAGGACTACCCGCCAGGGTGATGACACGCATACTC                                      <  SRR4302533.2545068/94‑1 (MQ=60)

                                                       CAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGGGCTGAAGCGAGAAGGAAAGCGAGGACTACCCGCCAGGGTGATGACACGCATACTC                                      >  SRR4302533.3410863/1‑94 (MQ=60)

                                                        AGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGGGCTGGAGCGAGAAGGAAAGCGAGGACTACCCGCCAGGGTGATGACACGCATACTCT                                     >  SRR4302533.673869/1‑94 (MQ=60)

                                                         GGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGGGCTGGAGCGAGAAGGAAAGCGAGGACTACCCGCCAGGGTGATGACACGCATACTCTC                                    >  SRR4302533.6073475/1‑94 (MQ=60)

                                                          GCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGGGCTGGAGCGAGAAGGAAAGCGAGGACTACCCGCCAGGGTGATGACACGCATACTCTCT                                   <  SRR4302533.5196280/94‑1 (MQ=60)

                                                           CCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGGGCTGGAGCGAGAAGGAAAGCGAGGACTACCCGCCAGGGTGATGACACGCATACTCTCTC                                  >  SRR4302533.5296023/1‑94 (MQ=60)

                                                            CATTTAGTTTTTCCCGCGCATATTCCAGCAAGGGGCTGGAGCGAGAAGGAAAGCGAGGACTACCCGCCAGGGTGATGACACGCATACTCTCTCC                                 <  SRR4302533.2087209/94‑1 (MQ=60)

                                                             ATTTAGTTTTTCCCGCGCATATTCCAGCAAGGGGCTGGAGCGAGTAGGAAAGAGAGGACTACCCGCCAGGGTGATGACACGCATACTCTCTCCT                                >  SRR4302533.3254110/1‑94 (MQ=60)

                                                             ATTTAGTTTTTCCCGCGCATATTCCAGCAAGGGGCTGGAGCGAGAAGGAAAGCGAGGACTACCCGCCAGGGTGATGACACGCATACTCTCTCCT                                <  SRR4302533.879410/94‑1 (MQ=60)

                                                             ATTTAGTTTTTCCCGCGCATATTCCAGCAAGGGGCTGGAGCGAGAAGGAAAGCGAGGACTACCCGCCAGGGTGATGACACGCATACTCTCTCCT                                >  SRR4302533.5227172/1‑94 (MQ=60)

                                                              TTTAGTTTTTCCCGCGCATATTCCAGCAAGGGGCTGGAGCGAGAAGGAAAGCGAGGACTACCCGCCAGGGTGATGACACGCATACTCTCTCCTT                               >  SRR4302533.1870953/1‑94 (MQ=60)

                                                              TTTAGTTTTTCCCGCGCATATTCCAGCAAGGGGCTGGAGCGAGAAGGAAAGCGAGGACTACCCGCCAGGGTGATGACACGCATACTCTCTCCTT                               <  SRR4302533.2232189/94‑1 (MQ=60)

                                                                TAGTTTTTCCCGCGCATATTCCAGCAAGGGGCTGGAGCGAGAAGGAAAGCGAGGACTACCCGCCAGGGTGATGACACGCATACTCTCTCCTTAT                             <  SRR4302533.3100930/94‑1 (MQ=60)

                                                                TAGTTTTTCCCGCGCATATTCCAGCAAGGGGCTGGAGCGAGAAGGAAAGCGAGGACTACCCGCCAGGGTGATGACACGCATACTCTCTCCTTAT                             <  SRR4302533.5906459/94‑1 (MQ=60)

                                                                TAGTTTTTCCCGCGCATATTCCAGCAAGGGGCTGGAGCGAGAAGGAAAGCGAGGACTACCCGCCAGGGTGATGACACGCATACTCTCTCCTTAT                             <  SRR4302533.422938/94‑1 (MQ=60)

                                                                 AGTTTTTCCCGCGCATATTCCAGCAAGGGGCTGGAGCGAGAAGGAAAGCGAGGACTCCCCGCCAGGGTGATGACACGCATACTCTCTCCTTATA                            <  SRR4302533.1193819/94‑1 (MQ=60)

                                                                 AGTTTTTCCCGCGCATATTCCAGCAAGGGGCTGGAGCGAGAAGGAAAGCGAGGACTACCCGCCAGGGTGATGACACGCATACTCTCTCCTTATA                            <  SRR4302533.6042752/94‑1 (MQ=60)

                                                                  GTTTTTCCCGCGCATATTCCAGCAAGGGGCTGGAGCGAGAAGGAAAGCGAGGACTACCCGCCAGGGTGATGACACGCATACTCTCTCCTTATAA                           <  SRR4302533.303489/94‑1 (MQ=60)

                                                                   TTTTTCCCGCGCATATTCCAGCAAGGGGCTGGAGCGAGAAGGAAAGCGAGGACTACCCGCCAGGGTGATGACACGCATACTCTCTCCTTATAAC                          <  SRR4302533.3113120/94‑1 (MQ=60)

                                                                       TCCCGCGCATATTCCAGCAAGGGGCTGGAGCGAGAAGGAAAGCGAGGACTACCCGCCAGGGTGATGACACGCATACTCTCTCCTTATAACCAAT                      <  SRR4302533.3852599/94‑1 (MQ=60)

                                                                        CCCGCGCATATTCCAGCAAGGGGCTGGAGCGAGAAGGAAAGCGAGGACTACCCGCCAGGGTGATGACACGCATACTCTCTCCTTATAACCAATT                     <  SRR4302533.113312/94‑1 (MQ=60)

                                                                         CCNNNCNTATTCCAGCAAGGGGNTGGAGCGAGAAGGAAAGCGAGGACTACCCGCCAGGGTGATGACACGCATACTCTCTCCTTATAACCAATTG                    <  SRR4302533.3101708/94‑1 (MQ=60)

                                                                         CCGCGCATATTCCAGCAAGGGGCTGGAGCGAGAAGGAAAGCGAGGACTACCCGCCAGGGTGATGACACGCATACTCTCTCCTTATAACCAATTG                    >  SRR4302533.4628676/1‑94 (MQ=60)

                                                                         CCGCGCATATTCCAGCAAGGGGCTGGAGCGAGAAGGAAAGCGAGGACTACCCGCCAGGGTGATGACACGCATACTCTCTCCTTATAACCAATTG                    <  SRR4302533.3026116/94‑1 (MQ=60)

                                                                         CCGCGCATATTCCAGCAAGGGGCTGGAGCGAGAAGGAAAGCGAGGACTACCCGCCAGGGTGATGACACGCATACTCTCTCCTTATAACCAATTG                    <  SRR4302533.2519107/94‑1 (MQ=60)

                                                                         CCGCGCATATTCCAGCAAGGGGCTGGAGCGAGAAGGAAAGCGAGGACTACCCGCCAGGGTGATGACACGCATACTCTCTCCTTATAACCAATTG                    <  SRR4302533.1915996/94‑1 (MQ=60)

                                                                          CGCGCATATTCCAGCAAGGGGCTGGAGCGAGAAGGAAAGCGAGGACTACCCGCCAGGGTGATGACACGCATACTCTCTCCTTATAACCAATTGT                   <  SRR4302533.3499409/94‑1 (MQ=60)

                                                                           GCGCATATTCCAGCAAGGGGCTGGAGCGAGAAGGAAAGCGAGGACTACCCGCCAGGGTGATGACACGCATACTCTCTCCTTATAACCAATTGTT                  <  SRR4302533.3195874/94‑1 (MQ=60)

                                                                           GCGCATATTCCAGCAAGGGGCTGGAGCGAGAAGGAAAGCGAGGACTACCCGCCAGGGTGATGACACGCATACTCTCTCCTTATAACCAATTGTT                  <  SRR4302533.3989330/94‑1 (MQ=60)

                                                                           GCGCATATTCCAGCAAGGGGCTGGAGCGAGAAGGAAAGCGAGGACTACCCGCCAGGGGGATGACACGCATACTCTCTCCTTATAACCAATTGTT                  >  SRR4302533.2839835/1‑94 (MQ=60)

                                                                            CGCATATTCCAGCAAGGGGCTGGAGCGAGAAGGAAAGCGAGGACTACCCGCCAGGGTGATGACACGCATACTCTCTCCTTATAACCAATTGTTC                 >  SRR4302533.2914111/1‑94 (MQ=60)

                                                                             GCATATTCCAGCAAGGGGCTGGAGCGAGAAGGAAAGCGAGGACTACCCGCCAGGGTGATGACACGCATACTCTCTCCTTATAACCAATTGTTCT                >  SRR4302533.482781/1‑94 (MQ=60)

                                                                              CATATTCCAGCAAGGGGCTGGAGCGAGAAGGAAAGCGAGGACTACCCGCCAGGGTGATGACACGCATACTCTCTCCTTATAACCAATTGTTCTT               >  SRR4302533.5939023/1‑94 (MQ=60)

                                                                              CATATTCCAGCAAGGGGCTGGAGCGAGAAGGAAAGCGAGGACTACCCGCCAGGGTGATGACACGCATACTCTCTCCTTATAACCAATTGTTCTT               >  SRR4302533.4899285/1‑94 (MQ=60)

                                                                               ATATTCCAGCAAGGGGCTGGAGCGAGAAGGAAAGCGAGGACTACCCTCCAGGGTGATGACACGCATACTCTCTCCTTATAACCAATTGTTCTTT              >  SRR4302533.4967203/1‑94 (MQ=60)

                                                                               ATATTCCAGCAAGGGGCTGGAGCGAGAAGGAAAGCGAGGACTACCCGCCAGGGTGATGACACGCATACTCTCTCCTTATAACCAATTGTTCTTT              <  SRR4302533.601995/94‑1 (MQ=60)

                                                                               ATATTCCAGCAAGGGGCTGGAGCGAGAAGGAAAGCGAGGACTACCCGCCAGGGTGATGACACGCATACTCTCTCCTTATAACCAATTGTTCTTT              >  SRR4302533.4829398/1‑94 (MQ=60)

                                                                                TATTCCAGCAAGGGGCTGGAGCGAGAAGGAAAGCGAGGACTACCCGCCAGGGTGATGACACGCATACTCTCTCCTTATAACCAATTGTTCTTTT             <  SRR4302533.4880022/94‑1 (MQ=60)

                                                                                  TTCCAGCAAGGGGCTGGAGCGAGAAGGAAAGCGAGGACTACCCGCCAGGGTGATGACACGCATACTCTCTCCTTATAACCAATTGTTCTTTTTT           <  SRR4302533.808395/94‑1 (MQ=60)

                                                                                   TCCAGCAAGGGGCTGGAGCGAGAAGGAAAGCGAGGACTACCCGCCAGGGTGATGACACGCATACTCTCTCCTTATAACCAATTGTTCTTTTTTT          <  SRR4302533.2822667/94‑1 (MQ=60)

                                                                                    CCAGCAAGGGGCTGGAGCGAGAAGGAAAGCGAGGACTACCCGCCAGGGTGATGACACGCATACTCTCTCCTTATAACCAATTGTTCTTTTTTTG         >  SRR4302533.4084073/1‑94 (MQ=60)

                                                                                       GCAAGGGGCTGGAGCGAGAAGGAAAGCGAGGACTACCCGCCAGGGTGATGACACGCATACTCTCTCCTTATAACCAATTGTTCTTTTTTTGTTA      <  SRR4302533.2021726/94‑1 (MQ=60)

                                                                                        CAAGGGGCTGGAGCGAGAAGGAAAGCGAGGACTACCCGCCAGGGTGATGACACGCATACTCTCTCCTTATAACCAATTGTTCTTTTTTTGTTAA     <  SRR4302533.572119/94‑1 (MQ=60)

                                                                                        CAAGGGGCTGGAGCGAGAAGGAAAGCGAGGACTACCCGCCAGGGTGATGACACGCATACTCTCTCCTTATAACCAATTGTTCTTTTTTTGTTAA     >  SRR4302533.5567448/1‑94 (MQ=60)

                                                                                         AAGGGGCTGGAGCGAGAAGGAAAGCGAGGACTACCCGCCAGGGTGATGACACGCATACTCTCTCCTTATAACCAATTGTTCTTTTTTTGTTAAC    <  SRR4302533.3958374/94‑1 (MQ=60)

                                                                                           GGGGCTGGAGCGAGAAGGAAAGCGAGGACTACCCGCCAGGGTGATGACACGCATACTCTCTCCTTATAACCAATTGTTCTTTTTTTGTTAACAT  <  SRR4302533.5409771/94‑1 (MQ=60)

AAAGTAGATCTTCCGGGGCGAAGTTTTGCAGATTCCAGTGATAAACCTCTACATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGAGCTGGAGCGAGAAGGAAAGCGAGGACTACCCGCCAGGGTGATGACACGCATACTCTCTCCTTATAACCAATTGTTCTTTTTTTGTTAACAT  >  NC_000913/996591‑996775

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 10 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`