Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1624	250.6	12620048	98.0%	12367647	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,202,768	A→G	D97G (GAT→GGT)	ymfL →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,202,768	0	A	G	100.0%	785.4 / NA	243	D97G (GAT→GGT)	ymfL	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (127/116); total (127/116)

Only 100 of 251 total aligned reads displayed.

TTTTACAGCGTGCTGGTGGCACTCACTTCATTGCTGATGCTGTGGCGCAGTCTGCAAATGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGATGTGGACAACGCCGATATTAACCAGCGTCTGCTGGAAGTCATTGAACAGATCGGCAGTTATTCAAAACAGATTCGTTCAGCAATCGAA  >  NC_000913/1202678‑1202856

 tttACAGCGTGCTGGTGGCACTCACTTCATTGCTGATGCTGTGGCGCAGTCTGCAAATGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGgtgt                                                                                       <  1:92723/93‑1 (MQ=255)

  ttACAGCGTGCTGGTGGCACTCACTTCATTGCTGATGCTGTGGCGCAGTCTGCAAATGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGGtgtg                                                                                      <  1:394928/93‑1 (MQ=255)

    aCAGCGTGCTGGTGGCACTCACTTCATTGCTGATGCTGTGGCGCAGTCTGCAAATGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGGa                                                                                    <  1:2462369/93‑1 (MQ=255)

     cAGCGTGCTGGTGGCACTCACTTCATTGCTGATGCTGTGGCGCAGTCTGCAAATGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGGAc                                                                                   >  1:2143000/1‑93 (MQ=255)

      aGCGTGCTGGTGGCACTCACTTCATTGCTGATGCTGTGGCGCAGTCTGCAAATGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGGACa                                                                                  >  2:4708182/1‑93 (MQ=255)

       gCGTGCTGGTGGCACTCACTTCATTGCTGATGCTGTGGCGCAGTCTGCAAATGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGGACaa                                                                                 <  2:3477224/93‑1 (MQ=255)

           gCTGGTGGCACTCACTTCATTGCTGATGCTGTGGCGCAGTCTGCAAATGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGGACAACGcc                                                                             >  1:1169741/1‑93 (MQ=255)

           gCTGGTGGCACTCACTTCATTGCTGATGCTGTGGCGCAGTCTGCAAATGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGGACAACGcc                                                                             >  1:3923147/1‑93 (MQ=255)

           gCTGGTGGCACTCACTTCATTGCTGATGCTGTGGCGCAGTCTGCAAATGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGGACAACGcc                                                                             <  2:5119736/93‑1 (MQ=255)

           gCTGGTGGAACTCACTTCATTGCTGATGCTGTGGCGCAGTCTGCAAATGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGGACAACGcc                                                                             >  2:6136629/1‑93 (MQ=255)

            cTGGTGGCACTCACTTCATTGCTGATGCTGTGGCGCAGTCTGCAAATGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGGACAACGCCg                                                                            >  2:5615381/1‑93 (MQ=255)

             tggtggCACTCACTTCATTGCTGATGCTGTGGCGCAGTCTGCAAATGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGGACAACGCCGa                                                                           <  2:3988140/93‑1 (MQ=255)

             tggtggCACTCACTTCATTGCTGATGCTGTGGCGCAGTCTGCAAATGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGGACAACGCCGa                                                                           >  2:1156202/1‑93 (MQ=255)

                 ggCACTCACTTCATTGCTGATGCTGTGGCGCAGTCTGCAAATGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGGACAACGCCGATAtt                                                                       <  2:2846513/93‑1 (MQ=255)

                 ggCACTCACTTCATTGCTGATGCTGTGGCGCAGTCTGCAAATGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGGACAACGCCGATAtt                                                                       <  1:1284990/93‑1 (MQ=255)

                   cactcactTCATTGCTGATGCTGTGGCGCAGTCTGCAAATGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGGACAACGCCGATATTaa                                                                     >  1:5280868/1‑93 (MQ=255)

                    actcactTCATTGCTGATGCTGTGGCGCAGTCTGCAAATGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGGACAACGCCGATATTAAc                                                                    >  2:507835/1‑93 (MQ=255)

                    actcactTCATTGCTGATGCTGTGGCGCAGTCTGCAAATGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGGACAACGCCGATATTAAc                                                                    >  1:4058822/1‑93 (MQ=255)

                    actcactTCATTGCTGATGCTGTGGCGCAGTCTGCAAATGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGGACAACGCCGATATTAAc                                                                    <  2:1565616/93‑1 (MQ=255)

                     ctcactTCATTGCTGATGCTGTGGCGCAGTCTGCAAATGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGGACAACGCCGATATTAAcc                                                                   <  1:1987736/93‑1 (MQ=255)

                     ctcactTCATTGCTGATGCTGTGGCGCAGTCTGCAAATGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGGACAACGCCGATATTAAcc                                                                   <  1:3825715/93‑1 (MQ=255)

                      tcactTCATTGCTGATGCTGTGGCGCAGTCTGCAAATGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGGACAACGCCGATATTAACCa                                                                  >  1:571049/1‑93 (MQ=255)

                      tcactTCATTGCTGATGCTGTGGCGCAGTCTGCAAATGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGGACAACGCCGATATTAACCa                                                                  >  1:2633806/1‑93 (MQ=255)

                       cactTCATTGCTGATGCTGTGGCGCAGTCTGCAAATGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGGACAACGCCGATATTAACCAg                                                                 <  1:4691738/93‑1 (MQ=255)

                        actTCATTGCTGATGCTGTGGCGCAGTCTGCAAATGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGGACAACGCCGATATTAACCAGc                                                                <  2:2951076/93‑1 (MQ=255)

                        actTCATTGCTGATGCTGTGGCGCAGTCTGCAAATGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGGACAACGCCGATATTAACCAGc                                                                >  2:3623765/1‑93 (MQ=255)

                         ctTCATTGCTGATGCTGTGGCGCAGTCTGCAAATGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGGACAACGCCGATATTAACCAGCg                                                               >  2:3582238/1‑93 (MQ=255)

                           tCATTGCTGATGCTGTGGCGCAGTCTGCAAATGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGGACAACGCCGATATTAACCAGCGTc                                                             <  2:3066430/93‑1 (MQ=255)

                           tCATTGCTGATGCTGTGGCGCAGTCTGCAAATGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGGACAACGCCGATATTAACCAGCGTc                                                             <  2:5932312/93‑1 (MQ=255)

                            cATTGCTGATGCTGTGGCGCAGTCTGCAAATGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGGACAACGCCGATATTAACCAGCGTct                                                            <  1:1066797/93‑1 (MQ=255)

                            cATTGCTGATGCTGTGGCGCAGTCTGCAAATGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGGACAACGCCGATATTAACCAGCGTct                                                            <  1:5250976/93‑1 (MQ=255)

                             aTTGCTGATGCTGTGGCGCAGTCTGCAAATGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGGACAACGCCGATATTAACCAGCGTctg                                                           >  1:6080747/1‑93 (MQ=255)

                             aTTGCTGATGCTGTGGCGCAGTCTGCAAATGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGGACAACGCCGATATTAACCAGCGTctg                                                           >  2:4064897/1‑93 (MQ=255)

                             aTTGCTGATGCTGTGGCGCAGTCTGCAAATGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGGACAACGCCGATATTAACCAGCGTctg                                                           <  1:5147043/93‑1 (MQ=255)

                              ttGCTGATGCTGTGGCGCAGTCTGCAAGTGGCGTCTTTGTGTCTCTTCCTGACGTCGAGAGTGTGGACAACGCCGATATTAACCAGCGTctgc                                                          <  2:6300086/93‑1 (MQ=255)

                               tGCTGATGCTGTGGCGCAGTCTGCAAATGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGGACAACGCCGTTATTAACCAGCGTctgct                                                         >  1:3178467/1‑93 (MQ=255)

                               tGCTGATGCTGTGGCGCAGTCTGCAAATGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGGACAACGCCGATATTAACCAGCGTctgct                                                         <  2:4516306/93‑1 (MQ=255)

                                 cTGATGCTGTGGCGCAGTCTGCAAATGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGGACAACGCCGATATTAACCAGCGTCTGCTgg                                                       >  2:627185/1‑93 (MQ=255)

                                  tGATGCTGTGGCGCAGTCTGCAAATGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGGACAACGCCGATATTAACCAGCGTCTGCTGGa                                                      >  2:5458778/1‑93 (MQ=255)

                                   gATGCTGTGGCGCAGTCTGCAAATGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGGACAACGCCGATATTAACCAGCGTCTGCTGGaa                                                     >  1:1692036/1‑93 (MQ=255)

                                    aTGCTGTGGCGCAGTCTGCAAATGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGGACAACGCCGATATTAACCAGCGTCTGCTGGAAg                                                    <  1:1261339/93‑1 (MQ=255)

                                     tGCTGTGGCGCAGTCTGCAAATGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGGACAACGCCGATATTAACCAGCGTCTGCTGGAAGt                                                   >  1:4738444/1‑93 (MQ=255)

                                     tGCTGTGGCGCAGTCTGCAAATGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGGACAACGCCGATATTAACCAGCGTCTGCTGGAAGt                                                   >  2:2133332/1‑93 (MQ=255)

                                     tGCTGTGGCGCAGTCTGCAAATGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGGACAACGCCGATATTAACCAGCGTCTGCTGGAAGt                                                   >  1:6258438/1‑93 (MQ=255)

                                      gCTGTGGCGCAGTCTGCAAATGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGGACAACGCCGATATTAACCAGCGTCTGCTGGAAGTc                                                  >  2:5227247/1‑93 (MQ=255)

                                      gCTGTGGCGCAGTCTGCAAATGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGGACAACGCCGATATTAACCAGCGTCTGCTGGAAGTc                                                  >  1:2502709/1‑93 (MQ=255)

                                      gCTGTGGCGCAGTCTGCAAATGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGGACAACGCCGATATTAACCAGCGTCTGCTGGAAGTc                                                  >  1:4240883/1‑93 (MQ=255)

                                       cTGTGGCGCAGTCTGCAAATGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGGACAACGCCGATATTAACCAGCGTCTGCTGGAAGTCa                                                 >  2:2673416/1‑93 (MQ=255)

                                        tgtgGCGCAGTCTGCAAATGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGGACAACGCCGATATTAACCAGCGTCTGCTGGAAGTCAt                                                <  2:2835684/93‑1 (MQ=255)

                                        tgtgGCGCAGTCTGCAAATGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGGACAACGCCGATATTAACCAGCGTCTGCTGGAAGTCAt                                                >  1:2750079/1‑93 (MQ=255)

                                           ggCGCAGTCTGCAAATGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGGACAACGCCGATATTAACCAGCGTCTGCTGGAAGTCATTGa                                             <  1:4079675/93‑1 (MQ=255)

                                            gcgcAGTCTGCAAATGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGGACAACGCCGATATTAACCAGCGTCTGCTGGAAGTCATTGaa                                            <  1:5806142/93‑1 (MQ=255)

                                             cgcAGTCTGCAAATGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGGACAACGCCGATATTAACCAGCGTCTGCTGGAAGTCATTGAAc                                           <  1:5902127/93‑1 (MQ=255)

                                             cgcAGTCTGCAAATGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGGACAACGCCGATATTAACCAGCGTCTGCTGGAAGTCATTGAAc                                           <  1:3674156/93‑1 (MQ=255)

                                              gcAGTCTGCAAATGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGGACAACGCCGATATTAACCAGCGTCTGCTGGAAGTCATTGAACa                                          >  2:3491070/1‑93 (MQ=255)

                                               cAGTCTGCAAATGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGGACAACGCCGATATTAACCAGCGTCTGCTGGAAGTCATTGAACAg                                         >  2:3235941/1‑93 (MQ=255)

                                               cAGTCTGCAAATGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGGACAACGCCGATATTAACCAGCGTCTGCTGGAAGTCATTGAACAg                                         <  2:5753893/93‑1 (MQ=255)

                                               cAGTCTGCAAATGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGGACAACGCCGATATTAACCAGCGTCTGCTGGAAGTCATTGAACAg                                         <  2:1759648/93‑1 (MQ=255)

                                                aGTCTGCAAATGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGGACAACGCCGATATTAACCAGCGTCTGCTGGAAGTCATTGAACAGa                                        <  1:2927807/93‑1 (MQ=255)

                                                 gTCTGCAAATGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGGACAACGCCGATATTAACCAGCGTCTGCTGGAAGTCATTGAACAGAt                                       >  2:799032/1‑93 (MQ=255)

                                                 gTCTGCAAATGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGGACAACGCCGATATTAACCAGCGTCTGCTGGAAGTCATTGAACAGAt                                       <  2:1383236/93‑1 (MQ=255)

                                                 gTCTGCAAATGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGGACAACGCCGATATTAACCAGCGTCTGCTGGAAGTCATTGAACAGAt                                       <  1:506139/93‑1 (MQ=255)

                                                  tCTGCAAATGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGGACAACGCCGATATTAACCAGCGTCTGCTGGAAGTCATTGAACAGATc                                      <  2:5699186/93‑1 (MQ=255)

                                                   cTGCAAATGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGGACAACGCCGATATTAACCAGCGTCTGCTGGAAGTCATTGAACAGATCg                                     <  2:3552141/93‑1 (MQ=255)

                                                      cAAATGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGGACAACGCCGATATTAACCAGCGTCTGCTGGAAGTCATTGAACAGATCGGCa                                  >  1:5964198/1‑93 (MQ=255)

                                                      cAAATGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGGACAACGCCGATATTAACCAGCGTCTGCTGGAAGTCATTGAACAGATCGGCa                                  >  1:2064626/1‑93 (MQ=255)

                                                      cAAATGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGGACAACGCCGATATTAACCAGCGTCTGCTGGAAGTCATTGAACAGATCGGCa                                  >  2:4605162/1‑93 (MQ=255)

                                                       aaaTGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGGACAACGCCGATATTAACCAGCGTCTGCTGGAAGTCATTGAACAGATCGGCAg                                 <  2:1672278/93‑1 (MQ=255)

                                                        aaTGGCGTCTTTGTGTCTCTTCTTGACGTCGAGGGTGTGGACAACGCCGATATTAACCAGCGTCTGCTGGAAGTCATTGAACAGATCGGCAGt                                <  2:712056/93‑1 (MQ=255)

                                                        aaTGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGGACAACGCCGATATTAACCAGCGTCTGCTGGAAGTCATTGAACAGATCGGCAGt                                <  1:3249914/93‑1 (MQ=255)

                                                         aTGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGGACAACGCCGATATTAACCAGCGTCTGCTGGAAGTCATTGAACAGATCGGCAGtt                               >  1:1637145/1‑93 (MQ=255)

                                                          tGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGGACAACGCCGATATTAACCAGCGTCTGCTGGAAGTCATTGAACAGATCGGCAGTTa                              >  2:1125276/1‑93 (MQ=255)

                                                           ggCGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGGACAACGCCGATATTAACCAGCGTCCGCTGGAAGTCATTGAACCGATCGGCAGTTAt                             >  1:2992493/1‑93 (MQ=255)

                                                            gCGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGGACAACGCCGATATTAACCAGCGTCTGCTGGAAGTCATTGAACAGATCGGCAGTTAtt                            >  1:4343939/1‑93 (MQ=255)

                                                             cGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGGACAACGCCGATATTAACCAGCGTCTGCTGGAAGTCATTGAACAGATCGGCAGTTATTc                           >  1:5881776/1‑93 (MQ=255)

                                                              gTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGGACAACGCCGATATTAACCAGCGTCTGCTGGAAGTCATTGAACAGATCGGCAGTTATTCa                          >  2:2163325/1‑93 (MQ=255)

                                                               tCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGGACAACGCCGATATTAACCAGCGTCTGCTGGAAGTCATTGAACAGATCGGCAGTTATTCaa                         >  1:3583752/1‑93 (MQ=255)

                                                                  ttGTGTCTCTTCCTGACGTCGAGGGTGTGGACAACGCCGATATTAACCAGCGTCTGCTGGAAGTCATTGAACAGATCGGCAGTTATTCAAAAc                      <  2:6005136/93‑1 (MQ=255)

                                                                    gtgtCTCTTCCTGACGTCGAGGGTGTGGACAACGCCGATATTAACCAGCGTCTGCTGGAAGTCATTGAACAGATCGGCAGTTATTCAAAACAg                    <  2:4393775/93‑1 (MQ=255)

                                                                      gtCTCTTCCTGACGTCGAGGGTGTGGACAACGCCGATATTAACCAGCGTCTGCTGGAAGTCATTGAACAGATCGGCAGTTATTCAAAACAGAt                  >  2:4107295/1‑93 (MQ=255)

                                                                       tctcTTCCTGACGTCGAGGGTGTGGACAACGCCGATATTACCCAGCGTCTGCTGGAAGTCATTGAACAGATCGGCAGTTATTCAAAACAGAtt                 <  2:2424004/93‑1 (MQ=255)

                                                                       tctcTTCCTGACGTCGAGGGTGTGGACAACGCCGATATTAACCAGCGTCTGCTGGAAGTCATTGAACAGATCGGCAGTTATTCAAAACAGAtt                 >  1:49593/1‑93 (MQ=255)

                                                                       tctcTTCCTGACGTCGAGGGTGTGGACAACGCCGATATTAACCAGCGTCTGCTGGAAGTCATTGAACAGATCGGCAGGTATTCAAAACAGAtt                 >  1:3477629/1‑93 (MQ=255)

                                                                           ttCCTGACGTCGAGGGTGTGGACAACGCCGATATTAACCAGCGTCTGCTGGAAGTCATTGAACAGATCGGCAGTTATTCAAAACAGATTCGtt             >  2:2248074/1‑93 (MQ=255)

                                                                           ttCCTGACGTCGAGGGTGTGGACAACGCCGATATTAACCAGCGTCTGCTGGAAGTCATTGAACAGATCGGCAGTTATTCAAAACAGATTCGtt             <  2:1206177/93‑1 (MQ=255)

                                                                            tCCTGACGTCGAGGGTGTGGACAACGCCGATATTAACCAGCGTCTGCTGGAAGTCATTGAACAGATCGGCAGTTATTCAAAACAGATTCGTTc            <  1:1471885/93‑1 (MQ=255)

                                                                            tCCTGACGTCGAGGGTGTGGACAACGCCGATATTAACCAGCGTCTGCTGGAAGTCATTGAACAGATCGGCAGTTATTCAAAACAGATTCGTTc            <  1:2348563/93‑1 (MQ=255)

                                                                              cTGACGTCGAGGGTGTGGACAACGCCGATATTAACCAGCGTCTGCTGGAAGTCATTGAACAGATCGGCAGTTATTCAAAACAGATTCGTTCAg          >  2:1041593/1‑93 (MQ=255)

                                                                                 aCGTCGAGGGTGTGGACAACGCCGATATTAACCAGCGTCTGCTGGAAGTCATTGAACAGATCGGCAGTTATTCAAAACAGATTCGTTCAGCaa       >  2:101770/1‑93 (MQ=255)

                                                                                 aCGTCGAGGGTGTGGACAACGCCGATATTAACCAGCGTCTGCTGGAAGTCATTGAACAGATCGGCAGTTATTCAAAACAGATTCGTTCAGCaa       <  2:1861643/93‑1 (MQ=255)

                                                                                 aCGTCGAGGGTGTGGACAACGCCGATATTAACCAGCGTCTGCTGGAAGTCATTGAACAGATCGGCAGTTATTCAAAACAGATTCGTTCAGCaa       <  2:144566/93‑1 (MQ=255)

                                                                                 aCGTCGAGGGTGTGGACAACGCCGATATTAACCAGCGTCTGCTGGAAGTCATTGAACAGATCGGCAGTTATTCAAAACAGATTCGTTCAGCaa       <  2:263445/93‑1 (MQ=255)

                                                                                 aCGTCGAGGGTGTGGACAACGCCGATATTAACCAGCGTCTGCTGGAAGTCATTGAACAGATCGGCAGTTATTCAAAACAGATTCGTTCAGCaa       <  1:5508267/93‑1 (MQ=255)

                                                                                  cGTCGAGGGTGTGGACAACGCCGATATTAACCAGCGTCTGCTGGAAGTCATTGAACAGATCGGCAGTTATTCAAAACAGATTCGTTCAGCAAt      <  2:4972948/93‑1 (MQ=255)

                                                                                  cGTCGAGGGTGTGGACAACGCCGATATTAACCAGCGTCTGCTGGAAGTCATTGAACAGATCGGCAGTTATTCAAAACAGATTCGTTCAGCAAt      <  1:609723/93‑1 (MQ=255)

                                                                                   gTCGAGGGTGTGGACAACGCCGATATTAACCAGCGTCTGCTGGAAGTCATTGAACAGATCGGCAGTTATTCAAAACAGATTCGTTCAGCAATc     <  2:3347625/93‑1 (MQ=255)

                                                                                   gTCGAGGGTGTGGACAACGCCGATATTAACCAGCGTCTGCTGGAAGTCATTGAACAGATCGGCAGTTATTCAAAACAGATTCGTTCAGCAATc     <  1:5137492/93‑1 (MQ=255)

                                                                                    tCGAGGGTGTGGACAACGCCGATATTAACCAGCGTCTGCTGGAAGTCATTGAACAGATCGGCAGTTATTCAAAACAGATTCGTTCAGCAATCg    >  1:175473/1‑93 (MQ=255)

                                                                                      gAGGGTGTGGACAACGCCGATATTAACCAGCGTCTGCTGGAAGTCATTGAACAGATCGGCAGTTATTCAAAACAGATTCGTTCAGCAATCGaa  <  2:1274324/93‑1 (MQ=255)

                                                                                      gAGGGTGTGGACAACGCCGATATTAACCAGCGTCTGCTGGAAGTCATTGAACAGATCGGCAGTTATTCAAAACAGATTCGTTCAGCAATCGaa  >  1:716713/1‑93 (MQ=255)

TTTTACAGCGTGCTGGTGGCACTCACTTCATTGCTGATGCTGTGGCGCAGTCTGCAAATGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGATGTGGACAACGCCGATATTAACCAGCGTCTGCTGGAAGTCATTGAACAGATCGGCAGTTATTCAAAACAGATTCGTTCAGCAATCGAA  >  NC_000913/1202678‑1202856

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

GGTTTTACAGCGTGCTGGTGGCACTCACTTCATTGCTGATGCTGTGGCGCAGTCTGCAAATGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGATGTGGACAACGCCGATATTAACCAGCGTCTGCTGGAAGTCATTGAACAGATCGGCAGTTATTCAAAACAGATTCGTTCAGCAATCGAAGACGG  >  NC_000913/1202676‑1202861

GGTTTTACAGCGTGCTGGTAGCACTCACTTCATTGCTGATGCTGTGGCGCAGTCTGCAAATGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGGT                                                                                              <  SRR4302533.2554386/94‑1 (MQ=60)

 GTTTTACAGCGTGCTGGTGGCACTCACTTCATTGCTGATGCTGTGGCGCAGTCTGCAAATGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGGTG                                                                                             >  SRR4302533.452242/1‑94 (MQ=60)

 GTTTTACAGCGTGCTGGTGGCACTCACTTCATTGCTGATGCTGTGGCGCAGTCTGCAAATGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGGTG                                                                                             >  SRR4302533.5053990/1‑94 (MQ=60)

   TTTACAGCGTGCTGGTGGCACTCACTTCATTGCTGATGCTGTGGCGCAGTCTGCAAATGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTG                                                                                           <  SRR4302533.93310/94‑1 (MQ=60)

   TTTACAGCGTGCTGGTGGCACTCACTTCATTGCTGATGCTGTGGCGCAGTCTGCAAATGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTG                                                                                           <  SRR4302533.4300974/94‑1 (MQ=60)

    TTACAGCGTGCTGGTGGCACTCACTTCATTGCTGATGCTGTGGCGCAGTCTGCAAATGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGG                                                                                          <  SRR4302533.397322/94‑1 (MQ=60)

    TTACAGCGTGCTGGTGGCACTCACTTCATTGCTGATGCTGTGGCGCAGTCTGCAAATGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGG                                                                                          >  SRR4302533.1341678/1‑94 (MQ=60)

      ACAGCGTGCTGGTGGCACTCACTTCATTGCTGATGCTGTGGCGCAGTCTGCAAATGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGGAC                                                                                        <  SRR4302533.2477560/94‑1 (MQ=60)

      ACAGCGTGCTGGTGGCACTCACTTCATTGCTGATGCTGTGGCGCAGTCTGCAAATGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGGAC                                                                                        >  SRR4302533.2156202/1‑94 (MQ=60)

       CAGCGTGCTGGTGGCACTCACTTCATTGCTGATGCTGTGGCGCAGTCTGCAAATGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGGACA                                                                                       >  SRR4302533.2952163/1‑94 (MQ=60)

         GCGTGCTGGTGGCACTCACTTCATTGCTGATGCTGTGGCGCAGTCTGCAAATGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGGACAAC                                                                                     <  SRR4302533.5753166/94‑1 (MQ=60)

         GCGTGCTGGTGGCACTCACTTCATTGCTGATGCTGTGGCGCAGTCTGCAAATGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGGACAAC                                                                                     <  SRR4302533.392485/94‑1 (MQ=60)

          CGTGCTGGTGGCACTCACTTCATTGCTGATGCTGTGGCGCAGTCTGCAAATGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGGACAACG                                                                                    <  SRR4302533.5147914/94‑1 (MQ=60)

           GTGCTGGTGGCACTCACTTCATTGCTGATGCTGTGGCGCAGTCTGCAAATGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGGACAACGC                                                                                   >  SRR4302533.4771720/1‑94 (MQ=60)

           GTGCTGGTGGCACTCACTTCATTGCTGATGCTGTGGCGCAGTCTGCAAATGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGGACAACGC                                                                                   <  SRR4302533.3233995/94‑1 (MQ=60)

            TGCTGGTGGCACTCACTTCATTGCTGATGCTGTGGCGCAGTCTGCAAATGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGGACAACGCC                                                                                  >  SRR4302533.1176761/1‑94 (MQ=60)

            TGCTGGTGGCACTCACTTCATTGCTGATGCTGTGGCGCAGTCTGCAAATGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGGACAACGCC                                                                                  >  SRR4302533.3947100/1‑94 (MQ=60)

                GGTGGCACTCACTTCATTGCTGATGCTGTGGCGCAGTCTGCAAATGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGGACAACGCCGATA                                                                              <  SRR4302533.833445/94‑1 (MQ=60)

                GGTGGCACTCACTTCATTGCTGATGCTGTGGCGCAGTCTGCAAATGGCGTCTTTGTGTCTCTTCCTGAAGTCGAGGGTGTGGACAACGCCGATA                                                                              >  SRR4302533.6122702/1‑94 (MQ=60)

                  TGGCACTCACTTCATTGCTGATGCTGTGGCGCAGTCTGCAAATGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGGACAACGCCGATATT                                                                            >  SRR4302533.4145927/1‑94 (MQ=60)

                   GGCACTCACTTCATTGCTGATGCTGTGGCGCAGTCTGCAAATGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGGACAACGCCGATATTA                                                                           <  SRR4302533.1292720/94‑1 (MQ=60)

                    GCACTCACTTCATTGCTGATGCTGTGGCGCAGTCTGCAAATGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGGACAACGCCGATATTAA                                                                          >  SRR4302533.5312511/1‑94 (MQ=60)

                     CACTCACTTCATTGCTGATGCTGTGGCGCAGTCTGCAAATGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGGACAACGCCGATATTAAC                                                                         >  SRR4302533.2096171/1‑94 (MQ=60)

                     CACTCACTTCATTGCTGATGCTGTGGCGCAGTCTGCAAATGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGGACAACGCCGATATTAAC                                                                         >  SRR4302533.4083529/1‑94 (MQ=60)

                      ACTCACTTCATTGCTGATGCTGTGGCGCAGTCTGCAAATGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGGACAACGCCGATATTAACC                                                                        <  SRR4302533.2312856/94‑1 (MQ=60)

                       CTCACTTCATTGCTGATGCTGTGGCGCAGTCTGCAAATGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGGACAACGCCGATATTAACCA                                                                       <  SRR4302533.1999894/94‑1 (MQ=60)

                       CTCACTTCATTGCTGATGCTGTGGCGCAGTCTGCAAATGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGGACAACGCCGATATTAACCA                                                                       <  SRR4302533.3849104/94‑1 (MQ=60)

                       CTCACTTCATTGCTGATGCTGTGGCGCAGTCTGCAAATGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGGACAACGCCGATATTAACCA                                                                       >  SRR4302533.574421/1‑94 (MQ=60)

                       CTCACTTCATTGCTGATGCTGTGGCGCAGTCTGCAAATGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGGACAACGCCGATATTAACCA                                                                       >  SRR4302533.2649992/1‑94 (MQ=60)

                        TCACTTCATTGCTGATGCTGTGGCGCAGTCTGCAAATGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGGACAACGCCGATATTAACCAG                                                                      <  SRR4302533.6091463/94‑1 (MQ=60)

                         CACTTCATTGCTGATGCTGTGGCGCAGTCTGCAAATGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGGACAACGCCGCTATTAACCAGC                                                                     >  SRR4302533.2402144/1‑94 (MQ=60)

                         CACTTCATTGCTGATGCTGTGGCGCAGTCTGCAAATGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGGACAACGCCGATATTAACCAGC                                                                     <  SRR4302533.4720175/94‑1 (MQ=60)

                         CACTTCATTGCTGATGCTGTGGCGCAGTCTGCAAATGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGGACAACGCCGATATTAACCAGC                                                                     >  SRR4302533.3875451/1‑94 (MQ=60)

                          ACTTCATTGCTGATGCTGTGGCGCAGTCTGCAAATGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGGACAACGCCGATATTAACCAGCG                                                                    >  SRR4302533.2130103/1‑94 (MQ=60)

                          ACTTCATTGCTGATGCTGTGGCGCAGTCTGCAAATGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGGACAACGCCGATATTAACCAGCG                                                                    >  SRR4302533.5286203/1‑94 (MQ=60)

                           CTTCATTGCTGATGCTGTGGCGCAGTCTGCAAATGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGGACAACGCCGATATTAACCAGCGT                                                                   >  SRR4302533.4820286/1‑94 (MQ=60)

                            TTCATTGCTGATGCTGTGGCGCAGTCTGCAAATGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGGACAACGCCGATATTAACCAGCGTC                                                                  >  SRR4302533.204121/1‑94 (MQ=60)

                            TTCATTGCTGATGCTGTGGCGCAGTCTGCAAATGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGGACAACGCCGATATTAACCAGCGTC                                                                  <  SRR4302533.1670201/94‑1 (MQ=60)

                            TTCATTGCTGATGCTGTGGCGCAGTCTGCAAATGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGGACAACGCCGATATTAACCAGCGTC                                                                  <  SRR4302533.5169921/94‑1 (MQ=60)

                            TTCATTGCTGATGCTGTGGCGCAGTCTGCAAATGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGGACAACGCCGATATTAAACAGCGTC                                                                  >  SRR4302533.525321/1‑94 (MQ=60)

                             TCATTGCTGATGCTGTGGCGCAGTCTGCAAATGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGGACAACGCCGATATTAACCAGCGTCT                                                                 <  SRR4302533.5525807/94‑1 (MQ=60)

                              CATTGCTGATGCTGTGGCGCAGTCTGCAAATGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGGACAACGCCGATATTAACCAGCGTCTG                                                                <  SRR4302533.3693349/94‑1 (MQ=60)

                              CATTGCTGATGCTGTGGCGCAGTCTGCAAATGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGGACAACGCCGATATTAACCAGCGTCTG                                                                <  SRR4302533.5282529/94‑1 (MQ=60)

                              CATTGCTGATGCTGTGGCGCAGTCTGCAAATGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGGACAACGCCGATATTAACCAGCGTCTG                                                                >  SRR4302533.2744763/1‑94 (MQ=60)

                              CATTGCTGATGCTGTGGCGCAGTCTGCAAATGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGGACAACGCCGATATTAACCAGCGTCTG                                                                <  SRR4302533.1073066/94‑1 (MQ=60)

                              CATTGCTGATGCTGTGGCGCAGTCTGCAAATGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGGACAACGCCGATATTAACCAGCGTCTG                                                                >  SRR4302533.6117519/1‑94 (MQ=60)

                              CATTGCTGATGCTGTGGCGCAGTCTGCAAATGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGGACAACGCCGATATTAACCAGCGTCTG                                                                <  SRR4302533.2525682/94‑1 (MQ=60)

                               ATTGCTGATGCTGTGGCGCAGTCTGCAAATGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGGACAACGCCGATATTAACCAGCGTCTGC                                                               <  SRR4302533.5178146/94‑1 (MQ=60)

                                TTGCTGATGCTGTGGCGCAGTCTGCAAATGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGGACAACGCCGTTATTAACCAGCGTCTGCT                                                              >  SRR4302533.3198019/1‑94 (MQ=60)

                                TTGCTGATGCTGTGGCGCAGTCTGCAAATGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGGACAACGCCGATATTAACCAGCGTCTGCT                                                              >  SRR4302533.837287/1‑94 (MQ=60)

                                 TGCTGATGCTGTGGCGCAGTCTGCAAATGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGGACAACGCCGATATTAACCAGCGTCTGCTG                                                             <  SRR4302533.4030100/94‑1 (MQ=60)

                                  GCTGATGCTGTGGCGCAGTCTGCAAATGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGGACAACGCCGATATTAACCAGCGTCTGCTGG                                                            >  SRR4302533.1177436/1‑94 (MQ=60)

                                  GCTGATGCTGTGGCGCAGTCTGCAAATGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGGACAACGCCGATATTAACCAGCGTCTGCTGG                                                            <  SRR4302533.4754185/94‑1 (MQ=60)

                                    TGATGCTGTGGCGCAGTCTGCAAATGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGGACAACGCCGATATTAACCAGCGTCTGCTGGAA                                                          >  SRR4302533.1383630/1‑94 (MQ=60)

                                    TGATGCTGTGGCGCAGTCTGCAAATGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGGACAACGCCGATATTAACCAGCGTCTGCTGGAA                                                          >  SRR4302533.1702284/1‑94 (MQ=60)

                                     GATGCTGTGGCGCAGTCTGCAAATGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGGACAACGCCGATATTAACCAGCGTCTGCTGGAAG                                                         <  SRR4302533.512942/94‑1 (MQ=60)

                                     GATGCTGTGGCGCAGTCTGCAAATGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGGACAACGCCGATATTAACCAGCGTCTGCTGGAAG                                                         <  SRR4302533.5524256/94‑1 (MQ=60)

                                      ATGCTGTGGCGCAGTCTGCAAATGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGGACAACGCCGATATTAACCAGCGTCTGCTGGAAGT                                                        >  SRR4302533.4767175/1‑94 (MQ=60)

                                      ATGCTGTGGCGCAGTCTGCAAATGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGGACAACGCCGATATTAACCAGCGTCTGCTGGAAGT                                                        >  SRR4302533.6295862/1‑94 (MQ=60)

                                      ATGCTGTGGCGCAGTCTGCAAATGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGGACAACGCCGATATTAACCAGCGTCTGCTGGAAGT                                                        <  SRR4302533.1268882/94‑1 (MQ=60)

                                       TGCTGTGGCGCAGTCTGCAAATGTCGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGGACAACGCCGATATTAACCAGCGTCTGCTGGAAGTC                                                       <  SRR4302533.2487056/94‑1 (MQ=60)

                                       TGCTGTGGCGCAGTCTGCAAATGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGGACAACGCCGATATTAACCAGCGTCTGCTGGAAGTC                                                       >  SRR4302533.2518143/1‑94 (MQ=60)

                                       TGCTGTGGCGCAGTCTGCAAATGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGGACAACGCCGATATTAACCAGCGTCTGCTGGAAGTC                                                       >  SRR4302533.4266492/1‑94 (MQ=60)

                                       TGCTGTGGCGCAGTCTGCAAATGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGGACAACGCCGATATTAACCAGCGTCTGCTGGAAGTC                                                       <  SRR4302533.1155886/94‑1 (MQ=60)

                                        GCTGTGGCGCAGTCTGCAAATGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGGACAACGCCGATATTAACCAGCGTCTGCTGGAAGTCA                                                      >  SRR4302533.1716308/1‑94 (MQ=60)

                                         CTGTGGCGCAGTCTGCAAATGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGGACAACGCCGATATTAACCAGCGTCTGCTGGAAGTCAT                                                     <  SRR4302533.3531132/94‑1 (MQ=60)

                                         CTGTGGCGCAGTCTGCAAATGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGGACAACGCCGATATTAACCAGCGTCTGCTGGAAGTCAT                                                     >  SRR4302533.2766938/1‑94 (MQ=60)

                                          TGTGGCGCAGTCTGCAAATGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGGACAACGCCGATATTAACCAGCGTCTGCTGGAAGTCATT                                                    <  SRR4302533.2820345/94‑1 (MQ=60)

                                          TGTGGCGCAGTCTGCAAATGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGGAAAACGCCGATATTAACCAGCGTCTGCTGGAAGTCATT                                                    <  SRR4302533.2820346/94‑1 (MQ=60)

                                             GGCGCAGTCTGCAAATGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGGACAACGCCGATATTAACCAGCGTCTGCTGGAAGTCATTGAA                                                 <  SRR4302533.4104510/94‑1 (MQ=60)

                                              GCGCAGTCTGCAAATGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGGACAACGCCGATATTAACCAGCGTCTGCTGGAAGTCATTGAAC                                                <  SRR4302533.5841204/94‑1 (MQ=60)

                                               CGCAGTCTGCAAATGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGGACAACGCCGATATTAACCAGCGTCTGCTGGAAGTCATTGAACA                                               <  SRR4302533.5937768/94‑1 (MQ=60)

                                               CGCAGTCTGCAAATGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGGACAACGCCGATATTAACCAGCGTCTGCTGGAAGTCATTGAACA                                               <  SRR4302533.3696738/94‑1 (MQ=60)

                                                GCAGTCTGCAAATGGCGTCTTTGTGTCTCTTCCTGCCGTCGAGGGTGTGGACAACGCCGATATTAACCAGCGTCTGCTGGAAGTCATTGAACAG                                              <  SRR4302533.1205482/94‑1 (MQ=60)

                                                GCAGTCTGCAAATGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGGACAACGCCGATATTAACCAGCGTCTGCTGGAAGTCATTGAACAG                                              >  SRR4302533.3745564/1‑94 (MQ=60)

                                                  AGTCTGCAAATGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGGACAACGCCGATATTAACCAGCGTCTGCTGGAAGTCATTGAACAGAT                                            <  SRR4302533.2945720/94‑1 (MQ=60)

                                                   GTCTGCAAATGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGGACAACGCCGATATTAACCAGCGTCTGCTGGAAGTCATTGAACAGATC                                           <  SRR4302533.3274603/94‑1 (MQ=60)

                                                   GTCTGCAAATGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGGACAACGCCGATATTAACCAGCGTCTGCTGGAAGTCATTGAACAGATC                                           <  SRR4302533.6197121/94‑1 (MQ=60)

                                                   GTCTGCAAATGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGGACAACGCCGATATTAACCAGCGTCTGCTGGAAGTCATTGAACAGATC                                           <  SRR4302533.509143/94‑1 (MQ=60)

                                                    TCTGCAAATGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGGACAACGCCGATATTAACCAGCGTCTGCTGGAAGTCATTGAACAGATCG                                          >  SRR4302533.5935657/1‑94 (MQ=60)

                                                       GCAAATGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGGCCAACGCCGATATTAACCAGCGTCTGCTGGAAGTCATTGAACAGCTCGGCA                                       >  SRR4302533.6000283/1‑94 (MQ=60)

                                                       GCAAATGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGGACAACGCCGATATTAACCAGCGTCTGCTGGAAGTCATTGAACAGATCGGCA                                       >  SRR4302533.6000284/1‑94 (MQ=60)

                                                       GCAAATGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGGACAACGCCGATATTAACCAGCGTCTGCTGGAAGTCATTGAACAGATCGGCA                                       >  SRR4302533.2077352/1‑94 (MQ=60)

                                                        CAAATGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGGACAACGCCGATATTAACCAGCGTCTGCTGGAAGTCATTGAACAGATCGGCAG                                      >  SRR4302533.3109802/1‑94 (MQ=60)

                                                         AAATGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGGACAACGCCGATATTAACCAGCGTCTGCTGGAAGTCATTGAACAGATCGGCAGT                                     <  SRR4302533.6086906/94‑1 (MQ=60)

                                                         AAATGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGGAGTGGACAACGCCGATATTAACCAGCGTCTGCTGGAAGTCATTGAACAGCTCGGCAGT                                     >  SRR4302533.5898427/1‑94 (MQ=60)

                                                          AATGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGGACAACGCCGATATTAACCAGCGTCTGCTGGAAGTCATTGAACAGATCGGCAGTT                                    <  SRR4302533.3269953/94‑1 (MQ=60)

                                                          AATGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGGACAACGCCGATATTAACCAGCGTCTGCTGGAAGTCATTGAACAGATCGGCAGTT                                    >  SRR4302533.1647058/1‑94 (MQ=60)

                                                           ATGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGGACAACGCCGATATTAACCAGCGTCTGCTGGAAGTCATTGAACAGATCGGCAGTTA                                   >  SRR4302533.4005408/1‑94 (MQ=60)

                                                           ATGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGGACAACGCCGATATTAACCAGCGTCTGCTGGAAGTCATTGAACAGATCGGCAGTTA                                   <  SRR4302533.2524431/94‑1 (MQ=60)

                                                           ATGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGGACAACGCCGATATTAACCAGCGTCTGCTGGAAGTCATTGAACAGATCGGCAGTTA                                   >  SRR4302533.1541/1‑94 (MQ=60)

                                                            TGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGGACAACGCCGATATTAACCAGCGTCCGCTGGAAGTCATTGAACCGATCGGCAGTTAT                                  >  SRR4302533.3010788/1‑94 (MQ=60)

                                                             GGCGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGGACAACGCCGATATTAACCAGCGTCTGCTGGAAGTCATTGAACAGATCGGCAGTTATT                                 >  SRR4302533.4370117/1‑94 (MQ=60)

                                                              GCGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGGACAACGCCGATATTAACCAGCGTCTGCTGGAAGTCATTGAACAGATCGGCAGTTATTC                                >  SRR4302533.5917323/1‑94 (MQ=60)

                                                              GCGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGGACAACGCCGATATTAACCAGCGTCTGCTGGAAGTCATTGAACAGATCGGCAGTTATTC                                <  SRR4302533.178701/94‑1 (MQ=60)

                                                               CGTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGGACAACGCCGATATTAACCAGCGTCTGCTGGAAGTCATCGAACAGATCGGCAGTTATTCA                               >  SRR4302533.4111694/1‑94 (MQ=60)

                                                                GTCTTTGTGTCTCTTCCTGACGTCGAGGGTGTGGACAACGCCGATATTAACCAGCGTCTGCTGGAAGTCATTGAACAGATCGGCAGTTATTCAA                              >  SRR4302533.3605824/1‑94 (MQ=60)

                                                                     TGTGTCTCTTCCTGACGTCGAGGGTGTGGACAACGCCGATATTAACCAGCGTCTGCTGGAAGTCATTGAACAGATCGGCAGTTATTCAAAACAG                         >  SRR4302533.5926434/1‑94 (MQ=60)

                                                                      GTGTCTCTTCCTGACGTCGAGGGTGTGGACAACGCCGATATTAACCAGCGTCTGCTGGAAGTCATTGAACAGATCGGCAGTTATTCAAAACAGA                        >  SRR4302533.3195761/1‑94 (MQ=60)

                                                                       TGTCTCTTCCTGACGTCGAGGGTGTGGACAACGCCGATATTAACCAGCGTCTGCTGGAAGTCATTGAACAGATCGGCAGTTATTCAAAACAGAT                       >  SRR4302533.469310/1‑94 (MQ=60)

                                                                        GTCTCTTCCTGACGTCGAGGGTGTGGACAACGCCGATATTAACCAGCGTCTGCTGGAAGTCATTGAACAGATCGGCAGTTATTCAAAACAGATT                      >  SRR4302533.49935/1‑94 (MQ=60)

                                                                        GTCTCTTCCTGACGTCGAGGGTGTGGACAACGCCGATATTAACCAGCGTCTGCTGGAAGTCATTGAACAGATCGGCAGTTATTCAAAACAGATT                      <  SRR4302533.1003982/94‑1 (MQ=60)

                                                                        GTCTCTTCCTGACGTCGAGGGTGTGGACAACGCCGATATTAACCAGCGTCTGCTGGAAGTCATTGAACAGATCGGCAGGTATTCAAAACAGATT                      >  SRR4302533.3499087/1‑94 (MQ=60)

                                                                        GTCTCTTCCTGACGTCGAGGGAGTGGACAACGCCGATATTAACCAGCGTCTGCTGGAAGTCAGTGAACAGATCGGGAGTTATTCAAAAGAGAGT                      >  SRR4302533.325849/1‑94 (MQ=60)

                                                                         TCTCTTCCTGACGTCGAGGGTGTGGACAACGCCGATGTTAACCAGCGTCTGCTGGAAGTCATTGAACAGATCGGCAGTTATTCAAAACAGATTC                     <  SRR4302533.127259/94‑1 (MQ=60)

                                                                          CTCTTCCTGACGTCGAGGGTGTGGACAACGCCGATATTAACCAGCGTCTGCTGGAAGTCATTGAACAGATCGGCAGTTATTCAAAACAGATTCG                    >  SRR4302533.630776/1‑94 (MQ=60)

                                                                           TCTTCCTGACGTCGAGGGTGTGGACAACGCCGATATTAACCAGCGTCTGCTGGAAGTCATTGAACAGATCGGCAGTTATTCAAAACAGATTCGT                   >  SRR4302533.166026/1‑94 (MQ=60)

                                                                             TTCCTGACGTCGAGGGTGTGGACAACGCCGATATTAACCAGCGTCTGCTGGAAGTCATTGAACAGATCGGCAGTTATTCAAAACAGATTCGTTC                 <  SRR4302533.5242786/94‑1 (MQ=60)

                                                                             TTCCTGACGTCGAGGGTGTGGACAACGCCGATATTAACCAGCGTCTGCTGGAAGTCATTGAACAGATCGGCAGTTATTCAAAACAGATTCGTTC                 >  SRR4302533.5743653/1‑94 (MQ=60)

                                                                              TCCTGACGTCGAGGGTGTGGACAACGCCGATATTAACCAGCGTCTGCTGGAAGTCATTGAACAGATCGGCAGTTATTCAAAACAGATTCGTTCA                <  SRR4302533.2363095/94‑1 (MQ=60)

                                                                              TCCTGACGTCGAGGGTGTGGACAACGCCGATATTAACCAGCGTCTGCTGGAAGTCATTGAACAGATCGGCAGTTATTCAAAACAGATTCGTTCA                <  SRR4302533.1480782/94‑1 (MQ=60)

                                                                               CCTGACGTCGAGTGTGTGGACAACGCCGATATTAACCAGCGTCTGCTGGAAGTCATTGAACAGATCGGCAGTTATTCAAAACAGATTCGTTCAG               <  SRR4302533.1919710/94‑1 (MQ=60)

                                                                                CTGACGTCGAGGGTGTGGACAACGCCGATATTAACCAGCGTCTGCTGGAAGTCATTGAACAGATCGGCAGTTATTCAAAACAGATTCGTTCAGC              >  SRR4302533.5789182/1‑94 (MQ=60)

                                                                                 TGACGTCGAGGGTGTGGACAACGCCGATATTAACCAGCGTCTGCTGGAAGTCATTGAACAGATCGGCAGTTATTCAAAACAGATTCGTTCAGCA             >  SRR4302533.2383980/1‑94 (MQ=60)

                                                                                   ACGTCGAGGGTGTGGACAACGCCGATATTAACCAGCGTCTGCTGGAAGTCATTGAACAGATCGGCAGTTATTCAAAACAGATTCGTTCAGCAAT           <  SRR4302533.3670098/94‑1 (MQ=60)

                                                                                   ACGTCGAGGGTGTGGACAACGCCGATATTAACCAGCGTCTGCTGGAAGTCATTGAACAGATCGGCAGTTATTCAAAACAGATTCGTTCAGCAAT           <  SRR4302533.5541515/94‑1 (MQ=60)

                                                                                    CGTCGAGGGTGTGGACAACGCCGATATTAACCAGCGTCTGCTGGAAGTCATTGAACAGATCGGCAGTTATTCAAAACAGATTCGTTCAGCAATC          >  SRR4302533.3952981/1‑94 (MQ=60)

                                                                                    CGTCGAGGGTGTGGACAACGCCGATATTAACCAGCGTCTGCTGGAAGTCATTGAACAGATCGGCAGTTATTCAAAACAGATTCGTTCAGCAATC          >  SRR4302533.626688/1‑94 (MQ=60)

                                                                                    CGTCGAGGGTGTGGACAACGCCGATATTAACCAGCGTCTGCTGGAAGTCATTGAACAGATCGGCAGTTATTCAAAACAGATTCGTTCAGCAATC          <  SRR4302533.613309/94‑1 (MQ=60)

                                                                                    CGTCGAGGGTGTGGACAACGCCGATATTAACCAGCGTCTGCTGGAAGTCATTGAACAGATCGGCAGTTATTCAAAACAGATTCGTTCAGCAATC          <  SRR4302533.4092887/94‑1 (MQ=60)

                                                                                     GTCGAGGGTGTGGACAACGCCGATATTAACCAGCGTCTGCTGGAAGTCATTGAACAGATCGGCAGTTATTCAAAACAGATTCGTTCAGCAATCG         <  SRR4302533.5168540/94‑1 (MQ=60)

                                                                                     GTCGAGGGTGTGGACAACGCCGATATTAACCAGCGTCTGCTGGAAGTCATTGAACAGATCGGCAGTTATTCAAAACAGATTCGTTCAGCAATCG         >  SRR4302533.176578/1‑94 (MQ=60)

                                                                                     GTCGAGGGTGTGGACAACGCCGATATTAACCAGCGTCTGCTGGAAGTCATTGAACAGATCGGCAGTTATTCAAAACAGATTCGTTCAGCAATCG         <  SRR4302533.3378723/94‑1 (MQ=60)

                                                                                       CGAGGGTGTGGACAACGCCGATATTAACCAGCGTCTGCTGGAAGTCATTGAACAGATCGGCAGTTATTCAAAACAGATTCGTTCAGCAATCGAA       >  SRR4302533.720938/1‑94 (MQ=60)

                                                                                       CGAGGGTGTGGACAACGCCGATATTAACCACCGTCTGCTGGGAGTGATTGAACAGATCGGaagatatttcaaacagatatgtttaacaatacaa       >  SRR4302533.5483125/1‑60 (MQ=52)

                                                                                         AGGGTGTGGACAACGCCGATATTAACCAGCGTCTGCTGGAAGTCATTGAACAGATCGGCAGTTATTCAAAACAGATTCGTTCAGCAATCGAAGA     >  SRR4302533.2661098/1‑94 (MQ=60)

                                                                                         AGGGTGTGGACAACGCCGATATTAACCAGCGTCTGCTGGAAGTCATTGAACAGATCGGCAGTTATTCAAAACAGATTCGTTCAGCAATCGAAGA     >  SRR4302533.1793503/1‑94 (MQ=60)

                                                                                         AGGGTGTGGACAACGCCGATATTAACCAGCGTCTGCTGGAAGTCATTGAACAGATCGGCAGTTATTCAAAACAGATTCGTTCAGCAATCGAAGA     >  SRR4302533.5824368/1‑94 (MQ=60)

                                                                                          GGGTGTGGACAACGCCGATATTAACCAGCGTCTGCTGGAAGTCATTGAACAGATCGGCAGTTATTCAAAACAGATTCGTTCAGCAATCGCAGAC    >  SRR4302533.3231243/1‑94 (MQ=60)

                                                                                          GGGTGTGGACAACGCCGATATTAACCAGCGTCTGCTGGAAGTCATTGAACAGATCGGCAGTTATTCAAAACAGATTCGTTCAGCAATCGAAGAC    >  SRR4302533.4386079/1‑94 (MQ=60)

                                                                                           GGTGTGGACAACGCCGATATTAACCAGCGTCTGCTGGAAGTCATTGAACAGATCGGCAGTTATTCAAAACAGATTCGTTCAGCAATCGAAGACG   >  SRR4302533.2915375/1‑94 (MQ=60)

                                                                                           GGTGTGGACAACGCCGATATTAACCAGCGTCTGCTGGAAGTCATTGAACAGATCGGCAGTTATTCAAAACAGATTCGTTCAGCAATCGAAGACG   <  SRR4302533.3639344/94‑1 (MQ=60)

                                                                                            GTGTGGACAACGCCGATATTAACCAGCGTCTGCTGGAAGTCATTGAACAGATCGGCAGTTATTCAAAACAGATTCGTTCAGCAATCGAAGACGG  >  SRR4302533.4384569/1‑94 (MQ=60)

                                                                                            GTGTGGACAACGCCGATATTAACCAGCGTCTGCTGGAAGTCATTGAACAGATCGGCAGTTATTCAAAACAGATTCGTTCAGCAATCGAAGACGG  <  SRR4302533.2450244/94‑1 (MQ=60)

GGTTTTACAGCGTGCTGGTGGCACTCACTTCATTGCTGATGCTGTGGCGCAGTCTGCAAATGGCGTCTTTGTGTCTCTTCCTGACGTCGAGGATGTGGACAACGCCGATATTAACCAGCGTCTGCTGGAAGTCATTGAACAGATCGGCAGTTATTCAAAACAGATTCGTTCAGCAATCGAAGACGG  >  NC_000913/1202676‑1202861

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 8 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`