Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,398,840 +C coding (964/1533 nt) ydaH ← putative pump protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,398,8381.C99.5% 634.4 / ‑5.7 198G322G (GGC→GGG) ydaHputative pump protein
Reads supporting (aligned to +/- strand):  ref base . (0/1);  major base C (69/127);  minor base T (1/0);  total (70/128)
Fisher's exact test for biased strand distribution p-value = 3.55e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

Only 100 of 217 total aligned reads displayed.

AAAACCATCACGATAAATCCCGCCATCTCTTTCATCGGTTCAATCATTAAATGCGGTAAATCCGCCTGACGTCGAATTGTGCGGGTAGCGATG‑CCATAAGCCAGCGAGACAACAAAGAAAAAAAGAATGATCAGTGGCACGATACCTTTAATAAAGGGTGATGGCATCACGGTGTGATT  >  NC_000913/1398746‑1398924
                                                                                             |                                                                                      
aaaaCCATCACGATAAATCCCGCCATCTCTTTCATCGGTTCAATCATTAAATGCGGTAAATCCGCCTGACGTCGAATTGTGCGGGTAGCGATg                                                                                         >  2:2688052/1‑93 (MQ=255)
    ccATCACGATAAATCCCGCCATCTCTTTCATCGGTTCAATCATTAAATGCGGTAAATCCGCCTGACGTCGAATTGTGCGGGTAGCGATG‑ccca                                                                                    >  2:1301465/1‑91 (MQ=255)
     cATCACGATAAATCCCGCCATCTCTTTCATCGGTTCAATCATTAAATGCGGTAAATCCGCCTGACGTCGAATTGTGCGGGTAGCGATG‑cccat                                                                                   <  1:5300637/93‑4 (MQ=255)
     cATCACGATAAATCCCGCCATCTCTTTCATCGGTTCAATCATTAAATGCGGTAAATCCGCCTGACGTCGAATTGTGCGGGTAGCGATG‑cccat                                                                                   <  1:3325758/93‑4 (MQ=255)
      aTCACGATAAATCCCGCCATCTCTTTCATCGGTTCAATCATTAAATGCGGTAAATCCGCCTGACGTCGAATTGTGCGGGTAGCGATG‑cccata                                                                                  >  2:3778140/1‑89 (MQ=255)
      aTCACGATAAATCCCGCCATCTCTTTCATCGGTTCAATCATTAAATGCGGTAAATCCGCCTGACGTCGAATTGTGCGGGTAGCGATG‑cccata                                                                                  >  1:3165896/1‑89 (MQ=255)
        cACGATAAATCCCGCCATCTCTTTCATCGGTTCAATCATTAAATGCGGTAAATCCGCCTGACGTCGAATTGTGCGGGTAGCGATGCCCATAAg                                                                                 >  2:5672356/1‑93 (MQ=255)
         aCGATAAATCCCGCCATCTCTTTCATCGGTTCAATCATTAAATGCGGTAAATCCGCCTGACGTCGAATTGTGCGGGTAGCGATGCCCATAAGc                                                                                <  1:1300739/93‑1 (MQ=255)
         aCGATAAATCCCGCCATCTCTTTCATCGGTTCAATCATTAAATGCGGTAAATCCGCCTGACGTCGAATTGTGCGGGTAGCGATGCCCATAAGc                                                                                >  1:4006996/1‑93 (MQ=255)
          cGATAAATCCCGCCATCTCTTTCATCGGTTCAATCATTAAATGCGGTAAATCCGCCTGACGTCGAATTGTGCGGGTAGCGATGCCCATAAGcc                                                                               <  1:4156588/93‑1 (MQ=255)
           gATAAATCCCGCCATCTCTTTCATCGGTTCAATCATTAAATGCGGTAAATCCGCCTGACGTCGAATTGTGCGGGTAGCGATGCCCATAAGCCa                                                                              >  1:6077424/1‑93 (MQ=255)
           gATAAATCCCGCCATCTCTTTCATCGGTTCAATCATTAAATGCGGTAAATCCGCCTGACGTCGAATTGTGCGGGTAGCGATGCCCATAAGCCa                                                                              <  1:1694125/93‑1 (MQ=255)
            aTAAATCCCGCCATCTCTTTCATCGGTTCAATCATTAAATGCGGTAAATCCGCCTGACGTCGAATTGTGCGGGTAGCGATGCCCATAAGCCAg                                                                             <  2:3035166/93‑1 (MQ=255)
            aTAAATCCCGCCATCTCTTTCATCGGTTCAATCATTAAATGCGGTAAATCCGCCTGACGTCGAATTGTGCGGGTAGCGATGCCCATAAGCCAg                                                                             <  1:5393466/93‑1 (MQ=255)
              aaaTCCCGCCATCTCTTTCATCGGTTCAATCATTAAATGCGGTAAATCCGCCTGACGTCGAATTGTGCGGGTAGCGATGCCCATAAGCCAGCg                                                                           <  1:4572915/93‑1 (MQ=255)
               aaTCCCGCCATCTCTTTCATCGGTTCAATCATTAAATGCGGTAAATCCGCCTGACGTCGAATTGTGCGGGTAGCGATGCCCATAAGCCAGCga                                                                          <  1:2513091/93‑1 (MQ=255)
                 tCCCGCCATCTCTTTCATCGGTTCAATCATTAAATGCGGTAAATCCGCCTGACGTCGAATTGTGCGGGTAGCGATGCCCATAAGCCAGCgaga                                                                        >  1:2158241/1‑93 (MQ=255)
                  cccGCCATCTCTTTCATCGGTTCAATCATTAAATGCGGTAAATCCGCCTGACGTCGAATTGTGCGGGTAGCGATGCCCATAAGCCAGCGAGac                                                                       <  2:4872115/93‑1 (MQ=255)
                  cccGCCATCTCTTTCATCGGTTCAATCATTAAATGCGGTAAATCCGCCTGACGTCGAATTGTGCGGGTAGCGATGCCCATAAGCCAGCGAGac                                                                       <  2:5327437/93‑1 (MQ=255)
                  cccGCCATCTCTTTCATCGGTTCAATCATTAAATGCGGTAAATCCGCCTGACGTCGAATTGTGCGGGTAGCGATGCCCATAAGCCAGCGAGac                                                                       >  1:4251124/1‑93 (MQ=255)
                   ccGCCATCTCTTTCATCGGTTCAATCATTAAATGCGGTAAATCCGCCTGACGTCGAATTGTGCGGGTAGCGATGCCCATAAGCCAGCGAGaca                                                                      <  2:472948/93‑1 (MQ=255)
                    cGCCATCTCTTTCATCGGTTCAATCATTAAATGCGGTAAATCCGCCTGACGTCGAATTGTGCGGGTAGCGATGCCCATAAGCCAGCGAGacaa                                                                     <  1:3607194/93‑1 (MQ=255)
                    cGCCATCTCTTTCATCGGTTCAATCATTAAATGCGGTAAATCCGCCTGACGTCGAATTGTGCGGGTAGCGATGCCCATAAGCCAGCGAGacaa                                                                     <  2:5536548/93‑1 (MQ=255)
                     gCCATCTCTTTCATCGGTTCAATCATTAAATGCGGTAAATCCGCCTGACGTCGAATTGTGCGGGTAGCGATGCCCATAAGCCAGCGAGacaac                                                                    <  2:2122738/93‑1 (MQ=255)
                     gCCATCTCTTTCATCGGTTCAATCATTAAATGCGGTAAATCCGCCTGACGTCGAATTGTGCGGGTAGCGATGCCCATAAGCCAGCGAGacaac                                                                    >  2:5105943/1‑93 (MQ=255)
                     gCCATCTCTTTCATCGGTTCAATCATTAAATGCGGTAAATCCGCCTGACGTCGAATTGTGCGGGTAGCGATGCCCATAAGCCAGCGAGacaac                                                                    <  2:4225688/93‑1 (MQ=255)
                      ccATCTCTTTCATCGGTTCAATCATTAAATGCGGTAAATCCGCCTGACGTCGAATTGTGCGGGTAGCGATGCCCATAAGCCAGCGAGacaaca                                                                   >  2:3961797/1‑93 (MQ=255)
                      ccATCTCTTTCATCGGTTCAATCATTAAATGCGGTAAATCCGCCTGACGTCGAATTGTGCGGGTAGCGATGCCCATAAGCCAGCGAGacaaca                                                                   >  2:662984/1‑93 (MQ=255)
                      ccATCTCTTTCATCGGTTCAATCATTAAATGCGGTAAATCCGCCTGACGTCGAATTGTGCGGGTAGCGATGCCCATAAGCCAGCGAGacaaca                                                                   <  1:2787629/93‑1 (MQ=255)
                        aTCTCTTTCATCGGTTCAATCATTAAATGCGGTAAATCCGCCTGACGTCGAATTGTGCGGGTAGCGATGCCCATAAGCCAGCGAGACAACaaa                                                                 <  2:1814151/93‑1 (MQ=255)
                        aTCTCTTTCATCGGTTCAATCATTAAATGCGGTAAATCCGCCTGACGTCGAATTGTGCGGGTAGCGATGCCCATAAGCCAGCGAGACAACaaa                                                                 >  2:1874715/1‑93 (MQ=255)
                          ctctTTCATCGGTTCAATCATTAAATGCGGTAAATCCGCCTGACGTCGAATTGTGCGGGTAGCGATGCCCATAAGCCAGCGAGACAACAAAGa                                                               <  2:381064/93‑1 (MQ=255)
                            ctTTCATCGGTTCAATCATTAAATGCGGTAAATCCGCCTGACGTCGAATTGTGCGGGTAGCGATGCCCATAAGCCAGCGAGACAACAAAGaaa                                                             >  2:3716981/1‑93 (MQ=255)
                             tttCATCGGTTCAATCATTAAATGCGGTAAATCCGCCTGACGTCGAATTGTGCGGGTAGCGATGCCCATAAGCCAGCGAGACAACAAAGaaaa                                                            >  2:1725280/1‑93 (MQ=255)
                               tCATCGGTTCAATCATTAAATGCGGTAAATCCGCCTGACGTCGAATTGTGCGGGTAGCGATGCCCATAAGCCAGCGAGACAACAAAGaaaaaa                                                          <  1:4815412/93‑1 (MQ=255)
                                cATCGGTTCAATCATTAAATGCGGTAAATCCGCCTGACGTCGAATTGTGCGGGTAGCGATGCCCATAAGCCAGCGAGACAACAAAGaaaaaaa                                                         <  2:4937394/93‑1 (MQ=255)
                                 aTCGGTTCAATCATTAAATGCGGTAAATCCGCCTGACGTCGAATTGTGCGGGTAGCGATGCCCATAAGCCAGCGAGACAACAAAGaaaaaaaa                                                        >  2:1125590/1‑92 (MQ=255)
                                 aTCGGTTCAATCATTAAATGCGGTAAATCCGCCTGACGTCGAATTGTGCGGGTAGCGATGCCCATAAGCCAGCGAGACAACAAAGAAAAAAAg                                                        <  1:3092369/93‑1 (MQ=255)
                                 aTCGGTTCAATCATTAAATGCGGTAAATCCGCCTGACGTCGAATTGTGCGGGTAGCGATGCCCATAAGCCAGCGAGACAACAAAGAAAAAAAg                                                        <  1:234496/93‑1 (MQ=255)
                                  tCGGTTCAATCATTAAATGCGGTAAATCCGCCTGACGTCGAATTGTGCGGGTAGCGATGCCCATAAGCCAGCGAGACAACAAAGAAAAAAAGa                                                       <  1:6214225/93‑1 (MQ=255)
                                  tCGGTTCAATCATTAAATGCGGTAAATCCGCCTGACGTCGAATTGTGCGGGTAGCGATGCCCATAAGCCAGCGAGACAACAAAGAAAAAAAGa                                                       <  1:1084891/93‑1 (MQ=255)
                                  tCGGTTCAATCATTAAATGCGGTAAATCCGCCTGACGTCGAATTGTGCGGGTAGCGATGCCCATAAGCCAGCGAGACAACAAAGAAAAAAAGa                                                       >  1:2108664/1‑93 (MQ=255)
                                   cGGTTCAATCATTAAATGCGGTAAATCCGCCTGACGTCGAATTGTGCGGGTAGCGATGCCCATAAGCCAGCGAGACAACAAAGAAAAAAAGaa                                                      <  2:3466752/93‑1 (MQ=255)
                                     gTTCAATCATTAAATGCGGTAAATCCGCCTGACGTCGAATTGTGCGGGTAGCGATGCCCATAAGCCAGCGAGACAACAAAGAAAAAAAGAATg                                                    >  1:2327817/1‑93 (MQ=255)
                                     gTTCAATCATTAAATGCGGTAAATCCGCCTGACGTCGAATTGTGCGGGTAGCGATGCCCATAAGCCAGCGAGACAACAAAGAAAAAAAGAATg                                                    <  2:1431699/93‑1 (MQ=255)
                                     gTTCAATCATTAAATGCGGTAAATCCGCCTGACGTCGAATTGTGCGGGTAGCGATGCCCATAAGCCAGCGAGACAACAAAGAAAAAAAGAATg                                                    >  1:466791/1‑93 (MQ=255)
                                     gTTCAATCATTAAATGCGGTAAATCCGCCTGACGTCGAATTGTGCGGGCAGCGATGCCCATAAGCCAGCGAGACAACAAAGAAAAAAAGAAta                                                    >  1:5611424/1‑92 (MQ=255)
                                      ttCAATCATTAAATGCGGTAAATCCGCCTGACGTCGAATTGTGCGGGTAGCGATGCCCATAAGCCAGCGAGACAACAAAGAAAAAAAGAATGa                                                   >  1:5770511/1‑93 (MQ=255)
                                      ttCAATCATTAAATGCGGTAAATCCGCCTGACGTCGAATTGTGCGGGTAGCGATGCCCATAAGCCAGCGAGACAACAAAGAAAAAAAGAATGa                                                   <  1:5195393/93‑1 (MQ=255)
                                      ttCAATCATTAAATGCGGTAAATCCGCCTGACGTCGAATTGTGCGGGTAGCGATGCCCATAAGCCAGCGAGACAACAAAGAAAAAAAGAATGa                                                   <  2:3880621/93‑1 (MQ=255)
                                       tCAATCATTAAATGCGGTAAATCCGCCTGACGTCGAATTGTGCGGGTAGCGATGCCCATAAGCCAGCGATACAACAAAGAAAAAAAGAATGAt                                                  >  2:1907833/1‑93 (MQ=255)
                                        cAATCATTAAATGCGGTAAATCCGCCTGACGTCGAATTGTGCGGGTAGCGATGCCCATAAGCCAGCGAGACAACAAAGAAAAAAAGAATGATc                                                 <  1:4592739/93‑1 (MQ=255)
                                         aaTCATTAAATGCGGTAAATCCGCCTGACGTCGAATTGTGCGGGTAGCGATGCCCATAAGCCAGCGAGACAACAAAGAAAAAAAGAATGATCa                                                <  1:4418839/93‑1 (MQ=255)
                                         aaTCATTAAATGCGGTAAATCCGCCTGACGTCGAATTGTGCGGGTAGCGATGCCCATAAGCCAGCGAGACAACAAAGAAAAAAAGAATGATCa                                                >  1:4746147/1‑93 (MQ=255)
                                         aaTCATTAAATGCGGTAAATCCGCCTGACGTCGAATTGTGCGGGTAGCGATGCCCATAAGCCAGCGAGACAACAAAGAAAAAAAGAATGATCa                                                <  1:5114313/93‑1 (MQ=255)
                                          aTCATTAAATGCGGTAAATCCGCCTGACGTCGAATTGTGCGGGTAGCGATGCCCATAAGCCAGCGAGACAACAAAGAAAAAAAGAATGATCAg                                               >  2:2393008/1‑93 (MQ=255)
                                           tCATTAAATGCGGTAAATCCGCCTGACGTCGAATTGTGCGGGTAGCGATGCCCATAAGCCAGCGAGACAACAAAGAAAAAAAGAATGATCAGt                                              <  1:4697511/93‑1 (MQ=255)
                                           tCATTAAATGCGGTAAATCCGCCTGACGTCGAATTGTGCGGGTAGCGATGCCCATAAGCCAGCGAGACAACAAAGAAAAAAAGAATGATCAGt                                              <  2:795964/93‑1 (MQ=255)
                                             aTTAAATGCGGTAAATCCGCCTGACGTCGAATTGTGCGGGTAGCGATGCCCATAAGCCAGCGAGACAACAAAGAAAAAAAGAATGATCAGTgg                                            >  2:4527003/1‑93 (MQ=255)
                                                  aTGCGGTAAATCCGCCTGACGTCGAATTGTGCGGGTAGCGATGCCCATAAGCCAGCGAGACAACAAAGAAAAAAAGAATGATCAGTGGCACGa                                       <  2:4402537/93‑1 (MQ=255)
                                                   tGCGGTAAATCCGCCTGACGTCGAATTGTGCGGGTAGCGATGCCCATAAGCCAGCGAGACAACAAAGAAAAAAAGAATGATCAGTGGCACGAt                                      >  1:3021885/1‑93 (MQ=255)
                                                   tGCGGTAAATCCGCCTGACGTCGAATTGTGCGGGTAGCGATGCCCATAAGCCAGCGAGACAACAAAGAAAAAAAGAATGATCAGTGGCACGAt                                      <  1:311924/93‑1 (MQ=255)
                                                    gCGGTAAATCCGCCTGACGTCGAATTGTGCGGGTAGCGATGCCCATAAGCCAGCGAGACAACAAAGAAAAAAAGAATGATCAGTGGCACGATa                                     <  1:5001242/93‑1 (MQ=255)
                                                     cGGTAAACCCGCCTGACGTCGAATTGTGCGGGTAGCGATGCCCATAAGCCAGCGAGACAACAAAGAAAAAAAGAATGATCAGTGGCACGATAc                                    <  2:4093058/93‑1 (MQ=255)
                                                      gttAAACCGGCTTGACGTCGAATGGTGCGGGTAGCGATGCCCATAAGCCAGCGAGACAACAAAGAAAAAAAGAATGATCAGTGGCACGATAcc                                   <  1:5962616/91‑1 (MQ=255)
                                                      ggTAAATCCGCCTGACGTCGAATTGTGCGGGTAGCGATGCCCATAAGCCAGCGAGACAACAAAGAAAAAAAGAATGATCAGTGGCACGATAcc                                   <  1:218001/93‑1 (MQ=255)
                                                      ggTAAATCCGCCTGACGTCGAATTGTGCGGGTAGCGATGCCCATAAGCCAGCGAGACAACAAAGAAAAAAAGAATGATCAGTGGCACGATAcc                                   <  1:748251/93‑1 (MQ=255)
                                                       gTAAATCCGCCTGACGTCGAATTGTGCGGGTAGCGATGCCCATAAGCCAGCGAGACAACAAAGAAAAAAAGAATGATCAGTGGCACGATACCt                                  >  2:5141711/1‑93 (MQ=255)
                                                         aaaTCCGCCTGACGTCGAATTGTGCGGGTAGCGATGCCCATAAGCCAGCGAGACAACAAAGAAAAAAAGAATGATCAGTGGCACGATACCttt                                <  2:1794032/93‑1 (MQ=255)
                                                         aaaTCCGCCTGACGTCGAATTGTGCGGGTAGCGATGCCCATAAGCCAGCGACACAACAAAGAAAAAAAGAATGATCAGTGGCACGATACCttt                                <  2:6111745/93‑1 (MQ=255)
                                                           aTCCGCCTGACGTCGAATTGTGCGGGTAGCGATGCCCATAAGCCAGCGAGACAACAAAGAAAAAAAGAATGATCAGTGGCACGATACCTTtaa                              <  2:5981515/93‑1 (MQ=255)
                                                              cGCCTGACGTCGAATTGTGCGGGTAGCGATGCCCATAAGCCAGCGAGACAACAAAGAAAAAAAGAATGATCAGTGGCACGATACCTTtaataa                           >  2:2313604/1‑93 (MQ=255)
                                                               gCCTGACGTCGAATTGTGCGGGTAGCGATGCCCATAAGCCAGCGAGACAACAAAGAAAAAAAGAATGATCAGTGGCACGATACCTTTAATaaa                          <  2:5333522/93‑1 (MQ=255)
                                                               gCCTGACGTCGAATTGTGCGGGTAGCGATGCCCATAAGCCAGCGAGACAACAAAGAAAAAAAGAATGATCAGTGGCACGATACCTTTAATaaa                          >  2:4270670/1‑93 (MQ=255)
                                                               gCCTGACGTCGAATTGTGCGGGTAGCGATGCCCATAAGCCAGCGAGACAACAAAGAAAAAAAGAATGATCAGTGGCACGATACCTTTAATaaa                          <  2:817644/93‑1 (MQ=255)
                                                               gCCTGACGTCGAATTGTGCGGGTAGCGATGCCCATAAGCCAGCGAGACAACAAAGAAAAAAAGAATGATCAGTGGCACGATACCTTTAATaaa                          >  1:4294011/1‑93 (MQ=255)
                                                                ccTGACGTCGAATTGTGCGGGTAGCGATGCCCATAAGCCAGCGAGACAACAAAGAAAAAAAGAATGATCAGTGGCACGATACCTTTAATAAAg                         >  2:1393664/1‑93 (MQ=255)
                                                                ccTGACGTCGAATTGTGCGGGTAGCGATGCCCATAAGCCAGCGAGACAACAAAGAAAAAAAGAATGATCAGTGGCACGATACCTTTAATAAAg                         <  1:588033/93‑1 (MQ=255)
                                                                ccTGACGTCGAATTGTGCGGGTAGCGATGCCCATAAGCCAGCGAGACAACAAAGAAAAAAAGAATGATCAGTGGCACGATACCTTTAATAAAg                         >  1:940870/1‑93 (MQ=255)
                                                                 cTGACGTCGAATTGTGCGGGTAGCGATGCCCATAAGCCAGCGAGACAACAAAGAAAAAAAGAATGATCAGTGGCACGATACCTTTAATAAAgg                        <  1:1021454/93‑1 (MQ=255)
                                                                  tGACGTCGAATTGTGCGGGTAGCGATGCCCATAAGCCAGCGAGACAACAAAGAAAAAAAGAATGATCAGTGGCACGATACCTTTAATAAAggg                       <  1:4985357/93‑1 (MQ=255)
                                                                  tGACGTCGAATTGTGCGGGTAGCGATGCCCATAAGCCAGCGAGACAACAAAGAAAAAAAGAATGATCAGTGGCACGATACCTTTAATAAAggg                       <  2:5877821/93‑1 (MQ=255)
                                                                  tGACGTCGAATTGTGCGGGTAGCGATGCCCATAAGCCAGCGAGACAACAAAGAAAAAAAGAATGATCAGTGGCACGATACCTTTAATAAAggg                       <  1:4388572/93‑1 (MQ=255)
                                                                  tGACGTCGAATTGTGCGGGTAGCGATGCCCATAAGCCAGCGAGACAACAAAGAAAAAAAGAATGATCAGTGGCACGATACCTTTAATAAAggg                       <  1:956698/93‑1 (MQ=255)
                                                                      gTCGAATTGTGCGGGTAGCGATGCCCATAAGCCAGCGAGACAACAAAGAAAAAAAGAATGATCAGTGGCACGATACCTTTAATAAAGGGTGAt                   <  1:4257102/93‑1 (MQ=255)
                                                                       tCGAATTGTGCGGGTAGCGATGCCCATAAGCCAGCGAGACAACAAAGAAAAAAAGAATGATCAGTGGCACGATACCTTTAATAAAGGGTGATg                  <  2:2546204/93‑1 (MQ=255)
                                                                       tCGAATTGTGCGGGTAGCGATGCCCATAAGCCAGCGAGACAACAAAGAAAAAAAGAATGATCAGTGGCACGATACCTTTAATAAAGGGTGATg                  <  2:5584765/93‑1 (MQ=255)
                                                                          aaTTGTGCGGGTAGCGATGCCCATAAGCCAGCGAGACAACAAAGAAAAAAAGAATGATCAGTGGCACGATACCTTTAATAAAGGGTGATGGCa               <  2:1374119/93‑1 (MQ=255)
                                                                            ttGTGCGGGTAGCGATGCCCATAAGCCAGCGAGACAACAAAGAAAAAAAGAATGATCAGTGGCACGATACCTTTAATAAAGGGTGATGGCATc             <  1:873092/93‑1 (MQ=255)
                                                                              gtgCGGGTAGCGATGCCCATAAGCCAGCGAGACAACAAAGAAAAAAAGAATGATCAGTGGCACGATACCTTTAATACAGGGTGATGGCATCAc           >  1:3862055/1‑93 (MQ=255)
                                                                              gtgCGGGTAGCGATGCCCATAAGCCAGCGAGACAACAAAGAAAAAAAGAATGATCAGTGGCACGATACCTTTAATAAAGGGTGATGGCATCAc           <  2:4797696/93‑1 (MQ=255)
                                                                               tgCGGGTAGCGATGCCCATAAGCCAGCGAGACAACAAAGAAAAAAAGAATGATCAGTGGCACGATACCTTTAATAAAGGGTGATGGCATCACg          <  1:4341219/93‑1 (MQ=255)
                                                                               ggCGGGTAGCGATGCCCATAAGCCAGCGAGACAACAAAGAAAAAAAGAATGATCAGTGGCACGATACCTTTAATAAAGGGTGATGGCATCACg          <  2:2798236/92‑1 (MQ=255)
                                                                                gCGGGTAGCGATGCCCATAAGCCAGCGAGACAACAAAGAAAAAAAGAATGATCAGTGGCACGATACCTTTAATAAAGGGTGATGGCATCACgg         <  1:5816456/93‑1 (MQ=255)
                                                                                 cGGGTAGCGATGCCCATAAGCCAGCGAGACAACAAAGAAAAAAAGAATGATCAGTGGCACGATACCTTTAATAAAGGGTGATGGCATCACGgt        <  1:1808307/93‑1 (MQ=255)
                                                                                  gggTAGCGATGCCCATAAGCCAGCGAGACAACAAAGAAAAAAAGAATGATCAGTGGCACGATACCTTTAATAAAGGGTGATGGCATCACGgtg       >  2:1586459/1‑93 (MQ=255)
                                                                                  gggTAGCGATGCCCATAAGCCAGCGAGACAACAAAGAAAAAAAGAATGATCAGTGGCACGATACCTTTAATAAAGGGTGATGGCATCACGgtg       <  1:1987278/93‑1 (MQ=255)
                                                                                   ggTAGCGATGCCCATAAGCCAGCGAGACAACAAAGAAAAAAAGAATGATCAGTGGCACGATACCTTTAATAAAGGGTGATGGCATCACGgtgt      <  1:1413614/93‑1 (MQ=255)
                                                                                    gTAGCGATGCCCATAAGCCAGCGAGACAACAAAGAAAAAAAGAATGATCAGTGGCACGATACCTTTAATAAAGGGTGATGGCATCACGGtgtg     <  2:3591621/93‑1 (MQ=255)
                                                                                       gCGATGCCCATAAGCCAGCGAGACAACAAAGAAAAAAAGAATGATCAGTGGCACGATACCTTTAATAAAGGGTGATGGCATCACGGTGTGAtt  >  1:3415634/1‑93 (MQ=255)
                                                                                             |                                                                                      
AAAACCATCACGATAAATCCCGCCATCTCTTTCATCGGTTCAATCATTAAATGCGGTAAATCCGCCTGACGTCGAATTGTGCGGGTAGCGATG‑CCATAAGCCAGCGAGACAACAAAGAAAAAAAGAATGATCAGTGGCACGATACCTTTAATAAAGGGTGATGGCATCACGGTGTGATT  >  NC_000913/1398746‑1398924

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ACCATCACGATAAATCCCGCCATCTCTTTCATCGGTTCAATCATTAAATGCGGTAAATCCGCCTGACGTCGAATTGTGCGGGTAGCGATG‑CCATAAGCCAGCGAGACAACAAAGAAAAAAAGAATGATCAGTGGCACGATACCTTTAATAAAGGGTGATGGCATCACGGTGTGATTAATCGGATC  >  NC_000913/1398749‑1398933
                                                                                            |                                                                                             
ACCATCACGATAAATCCCGCCATCTCTTTCATCGGTTCAATCATTAAATGCGGTAAATCCGCCTGACGTCGAATTGTGCGGGTAGCGATG‑CCca                                                                                             <  SRR4302533.5978016/94‑3 (MQ=60)
  CATCACGATAAATCCCGCCATCTCTTTCATCGGTTCAATCATTAAATGCGGTAAATCCGCCTGACGTCGAATTGTGCGGGTAGCGATGCCCATA                                                                                            >  SRR4302533.4868557/1‑94 (MQ=60)
  CATCACGATAAATCCCGCCATCTCTTTCATCGGTTCAATCATTAAATGCGGTAAATCCGCCTGACGTCGAATTGTGCGGGTAGCGATGCCCATA                                                                                            >  SRR4302533.3185377/1‑94 (MQ=60)
  CATCACGATAAATCCCGCCATCTCTTTCATCGGTTCAATCATTAAATGCGGTAAATCCGCCTGACGTCGAATTGTGCGGGTAGCGATGCCCATA                                                                                            <  SRR4302533.5332391/94‑1 (MQ=60)
  CATCACGATAAATCCCGCCATCTCTTTCATCGGTTCAATCATTAAATGCGGTAAATCCGCCTGACGTCGAATTGTGCGGGTAGCGATGCCCATA                                                                                            >  SRR4302533.3030190/1‑94 (MQ=60)
  CATCACGATAAATCCCGCCATCTCTTTCATCGGTTCAATCATTAAATGCGGTAAATCCGCCTGACGTCGAATTGTGCGGGTAGCGATGCCCATA                                                                                            >  SRR4302533.4868555/1‑94 (MQ=60)
  CATCACGATAAATCCCGCCATCTCTTTCATCGGTTCAATCATTAAATGCGGTAAATCCGCCTGACGTCGAATTGTGCGGGTAGCGATGCCCATA                                                                                            <  SRR4302533.3346307/94‑1 (MQ=60)
   ATCACGATAAATCCCGCCATCTCTTTCATCGGTTCAATCATTAAATGCGGTAAATCCGCCTGACGTCGAATTGTGCGGGTAGCGATGCCCATAA                                                                                           <  SRR4302533.4821871/94‑1 (MQ=60)
    TCACGATAAATCCCGCCATCTCTTTCATCGGTTCAATCATTAAATGCGGTAAATCCGCCTGACGTCGAATTGTGCGGGTAGCGATGCCCATAAG                                                                                          <  SRR4302533.2450951/94‑1 (MQ=60)
     CACGATAAATCCCGCCATCTCTTTCATCGGTTCAATCATTAAATGCGGTAAATCCGCCTGACGTCGAATTGTGCGGGTAGCGATGCCCATAAGC                                                                                         >  SRR4302533.4031390/1‑94 (MQ=60)
      ACGATAAATCCCGCCATCTCTTTCATCGGTTCAATCATTAAATGCGGTAAATCCGCCTGACGTCGAATTGTGCGGGTAGCGATGCCCATAAGCC                                                                                        <  SRR4302533.1308563/94‑1 (MQ=60)
       CGATAAATCCCGCCATCTCTTTCATCGGTTCAATCATTAAATGCGGTAAATCCGCCTGACGTCGAATTGTGCGGGTAGCGATGCCCATAAGCCA                                                                                       >  SRR4302533.6114181/1‑94 (MQ=60)
       CGATAAATCCCGCCATCTCTTTCATCGGTTCAATCATTAAATGCGGTAAATCCGCCTGACGTCGAATTGTGCGGGTAGCGATGCCCATAAGCCA                                                                                       <  SRR4302533.4181772/94‑1 (MQ=60)
       CGATAAATCCCGCCATCTCTTTCATCGGTTCAATCATTAAATGCGGTAAATCCGCCTGACGTCGAATTGTGCGGGTAGCGATGCCCATAAGCCA                                                                                       <  SRR4302533.4765219/94‑1 (MQ=60)
       CGATAAATCCCGCCATCTCTTTCATCGGTTCAATCATTAAATGCGGTAAATCCGCCTGACGTCGAATTGTGCGGGTAGCGATG‑CCATAAGCCAG                                                                                      <  SRR4302533.5823557/94‑1 (MQ=60)
        GATAAATCCCGCCATCTCTTTCATCGGTTCAATCATTAAATGCGGTAAATCCGCCTGACGTCGAATTGTGCGGGTAGCGATGCCCATAAGCCAG                                                                                      <  SRR4302533.1704387/94‑1 (MQ=60)
        GATAAATCCCGCCATCTCTTTCATCGGTTCAATCATTAAATGCGGTAAATCCGCCTGACGTCGAATTGTGCGGGTAGCGATGCCCATAAGCCAG                                                                                      <  SRR4302533.2174792/94‑1 (MQ=60)
         ATAAATCCCGCCATCTCTTTCATCGGTTCAATCATTAAATGCGGTAAATCCGCCTGACGTCGAATTGTGCGGGTAGCGATGCCCATAAGCCAGC                                                                                     <  SRR4302533.5425870/94‑1 (MQ=60)
          TAAATCCCGCCATCTCTTTCATCGGTTCAATCATTAAATGCGGTAAATCCGCCTGACGTCGAATTGTGCGGGTAGCGATGCCCATAAGCCAGCG                                                                                    <  SRR4302533.6032275/94‑1 (MQ=60)
           AAATCCCGCCATCTCTTTCATCGGTTCAATCATTAAATGCGGTAAATCCGCCTGACGTCGAATTGTGCGGGTAGCGATGCCCATAAGCCAGCGA                                                                                   <  SRR4302533.4600628/94‑1 (MQ=60)
           AAATCCCGCCATCTCTTTCATCGGTTCAATCATTAAATGCGGTAAATCCGCCTGACGTCGAATTGTGCGGGTAGCGATGCCCATAAGCCAGCGA                                                                                   <  SRR4302533.2205594/94‑1 (MQ=60)
            AATCCCGCCATCTCTTTCATCGGTTCAATCATTAAATGCGGTAAATCCGCCTGACGTCGAATTGTGCGGGTAGCGATGCCCATAAGCCAGCGAG                                                                                  <  SRR4302533.412510/94‑1 (MQ=60)
            AATCCCGCCATCTCTTTCATCGGTTCAATCATTAAATGCGGTAAATCCGCCTGACGTCGAATTGTGCGGGTAGCGATGCCCATAAGCCAGCGAG                                                                                  <  SRR4302533.2528576/94‑1 (MQ=60)
            AATCCCGCCATCTCTTTCATCGGTTCAATCATTAAATGCGGTAAATCCGCCTGACGTCGAATTGTGCGGGTAGCGATGCCCATAAGACAGCGAG                                                                                  >  SRR4302533.2694628/1‑94 (MQ=60)
             ATCCCGCCATCTCTTTCATCGGTTCAATCATTAAATGCGGTAAATCCGCCTGACGTCGAATTGTGCGGGTAGCGATGCCCATAAGCCAGCGAGA                                                                                 >  SRR4302533.2171526/1‑94 (MQ=60)
              TCCCGCCATCTCTTTCATCGGTTCAATCATTAAATGCGGTAAATCCGCCTGACGTCGAATTGTGCGGGTAGCGATGCCCATAAGCCAGCGAGAC                                                                                <  SRR4302533.5685498/94‑1 (MQ=60)
              TCCCGCCATCTCTTTCATCGGTTCAATCATTAAATGCGGTAAATCCGCCTGACGTCGAATTGTGCGGGTAGCGATGCCCATAAGCCAGCGAGAC                                                                                >  SRR4302533.4276770/1‑94 (MQ=60)
               CCCGCCATCTCTTTCATCGGTTCAATCATTAAATGCGGTAAATCCGCCTGACGTCGAATTGTGCGGGTAGCGATGCCCATAAGCCAGCGAGACA                                                                               >  SRR4302533.5608002/1‑94 (MQ=60)
                CCGCCATCTCTTTCATCGGTTCAATCATTAAATGCGGTAAATCCGCCTGACGTCGAATTGTGCGGGTAGCGATGCCCATAAGCCAGCGAGACAA                                                                              >  SRR4302533.2366750/1‑94 (MQ=60)
                 CGCCATCTCTTTCATCGGTTCAATCATTAAATGCGGTAAATCCGCCTGACGTCGAATTGTGCGGGTAGCGATGCCCATAAGCCAGCGAGACAAC                                                                             <  SRR4302533.3629400/94‑1 (MQ=60)
                   CCATCTCTTTCATCGGTTCAATCATTAAATGCGGTAAATCCGCCTGACGTCGAATTGTGCGGGTAGCGATGCCCATAAGCCAGCGAGACAACAA                                                                           <  SRR4302533.3715254/94‑1 (MQ=60)
                   CCATCTCTTTCATCGGTTCAATCATTAAATGCGGTAAATCCGCCTGACGTCGAATTGTGCGGGTAGCGATGCCCATAAGCCAGCGAGACAACAA                                                                           <  SRR4302533.2804758/94‑1 (MQ=60)
                   CCATCTCTTTCATCGGTTCAATCATTAAATGCGGTAAATCCGCCTGACGTCGAATTGTGCGGGTAGCGATGCCCATAAGCCAGCGAGACAACAA                                                                           >  SRR4302533.4488770/1‑94 (MQ=60)
                    CATCTCTTTCATCGGTTCAATCATTAAATGCGGTAAATCCGCCTGACGTCGAATTGTGCGGGTAGCGATGCCCATAAGCCAGCGAGACAACAAA                                                                          <  SRR4302533.3532251/94‑1 (MQ=60)
                    CATCTCTTTCATCGGTTCAATCATTAAATGCGGTAAATCCGCCTGACGTCGAATTGTGCGGGTAGCGATGCCCATAAGCCAGCGAGACAACAAA                                                                          <  SRR4302533.1592081/94‑1 (MQ=60)
                    CATCTCTTTCATCGGTTCAATCATTAAATGCGGTAAATCCGCCTGAAGTTGAATTGTGCGGGTAGCGATGCCCATAAGCCAGTGAGACAACAAA                                                                          >  SRR4302533.4236139/1‑94 (MQ=60)
                     ATCTCTTTCATCGGTTCAATCATTAAATGCGGTAAATCCGCCTGACGTCGAATTGTGCGGGTAGCGATGCCCATAAGCCAGCGAGACAACAAAG                                                                         <  SRR4302533.5582705/94‑1 (MQ=60)
                     ATCTCTTTCATCGGTTCAATCATTAAATGCGGTAAATCCGCCTGACGTCGAATTGTGCGGGTAGCGATGCCCATAAGCCAGCGAGACAACAAAG                                                                         >  SRR4302533.4278174/1‑94 (MQ=60)
                      TCTCTTTCATCGGTTCAATCATTAAATGCGGTAAATCCGCCTGACGTCGAATTGTGCGGGTAGCGATGCCCATAAGCCAGCGAGACAACAAACA                                                                        <  SRR4302533.4398445/94‑1 (MQ=60)
                       CTCTTTCATCGGTTCAATCATTAAATGCGGTAAATCCGCCTGACGTCGAATTGTGCGGGTAGCGATGCCCATAAGCCAGCGAGACAACAAAGAA                                                                       <  SRR4302533.5023580/94‑1 (MQ=60)
                         CTTTCATCGGTTCAATCATTAAATGCGGTAAATCCGCCTGACGTCGAATTGTGCGGGTAGCGATGCCCATAAGCCAGCGAGACAACAAAGAAAA                                                                     <  SRR4302533.5174328/94‑1 (MQ=60)
                            TCATCGGTTCAATCATTAAATGCGGTAAATCCGCCTGACGTCGAATTGTGCGGGTAGCGATGCCCATAAGCCAGCGAGACAACAAAGAAAAAAA                                                                  <  SRR4302533.4844598/94‑1 (MQ=60)
                              ATCGGTTCAATCATTAAATGCGGTAAATCCGCCTGACGTCGAATTGTGCGGGTAGCGATGCCCATAAGCCAGCGAGACAACAAAGAAAAAAAGA                                                                <  SRR4302533.3111383/94‑1 (MQ=60)
                              ATCGGTTCAATCATTAAATGCGGTAAATCCGCCTGACGTCGAATTGTGCGGGTAGCGATGCCCATAAGCCAGCGAGACAACAAAGAAAAAAAGA                                                                <  SRR4302533.235944/94‑1 (MQ=60)
                              ATCGGTTCAATCATTAAATGCGGTAAATCCGCCTGACGTCGAATTGTGCGGGTAGCGATGCCCATAAGCCAGCGAGACAACAAAGAAAAAAAGA                                                                >  SRR4302533.2121671/1‑94 (MQ=60)
                               TCGGTTCAATCATTAAATGCGGTAAATCCGCCTGACGTCGAATTGTGCGGGTAGCGATGCCCATAAGCCAGCGAGACAACAAAGAAAAAAAGAA                                                               <  SRR4302533.1091289/94‑1 (MQ=60)
                               TCGGTTCAATCATTAAATGCGGTAAATCCGCCTGACGTCGAATTGTGCGGGTAGCGATGCCCATAAGCCAGCGAGACAACAAAGAAAAAAAGAA                                                               <  SRR4302533.6251490/94‑1 (MQ=60)
                               TCGGTTCAATCATTAAATGCGGTAAATCCGCCTGACGTCGAATTGTGCGGGTAGCGATGCCCATAAGCCAGCGAGACAACAAAGAAAAAAAGAA                                                               <  SRR4302533.1090793/94‑1 (MQ=60)
                               TCGGTTCAATCATTAAATGCGGTAAATCCGCCTGACGTCGAATTGTGCGGGTAGCGATGCCCATAAGCCAGCGAGACAACAAAGAAAAAAAGAA                                                               <  SRR4302533.469846/94‑1 (MQ=60)
                                CGGTTCAATCATTAAATGCGGTAAATCCGCCTGACGTCGAATTGTGCGGGTAGCGATGCCCATAAGCCAGCGAGACAACAAAGAAAAAAAGAAT                                                              <  SRR4302533.3180628/94‑1 (MQ=60)
                                CGATTCAATCATTAAATGCGGTAAATCCGCCTGACGTCGAATTGTGCGGGTAGCGATGCCCATAAGCCAGCGAGACAACAAAGAAAAAAAGAAT                                                              <  SRR4302533.6189487/94‑1 (MQ=60)
                                 GGTTCAATCATTAAATGCGGTAAATCCGCCTGACGTCGAATTGTGCGGGTAGCGATGCCCATAAGCCAGCGAGACAACAAAGAAAAAAAGAATG                                                             >  SRR4302533.469579/1‑94 (MQ=60)
                                 GGTTCAATCATTAAATGCGGTAAATCCGCCTGACGTCGAATTGTGCGGGTAGCGATGCCCATAAGCCAGCGAGACAACAAAGAAAAAAAGAATG                                                             >  SRR4302533.2342226/1‑94 (MQ=60)
                                 GGTTCAATCATTAAATGCGGTAAATCCGCCTGACGTCGAATTGTGCGGGTAGCGATGCCCATAAGCCAGCGAGACAACAAAGAAAAAAAGAATG                                                             <  SRR4302533.5040415/94‑1 (MQ=60)
                                 GGTTCAATCATTAAATGCGGTAAATCCGCCTGACGTCGAATTGTGCGGGTAGCGATGCCCATAAGCCAGCGAGACAACAAAGAAAAAAAGAATG                                                             <  SRR4302533.957450/94‑1 (MQ=60)
                                 GGTTCAATCATTAAATGCGGTAAATCCGCCTGACGTCGAATTGTGCGGGCAGCGATGCCCATAAGCCAGCGAGACAACAAAGAAAAAAAGAATA                                                             >  SRR4302533.5645340/1‑94 (MQ=60)
                                  GTTCAATCATTAAATGCGGTAAATCCGCCTGACGTCGAATTGTGCGGGTAGCGATGCCCATAAGCCAGCGAGACAACAAAGAAAAAAAGAATGA                                                            >  SRR4302533.5805352/1‑94 (MQ=60)
                                  GTTCAATCATTAAATGCGGTAAATCCGCCTGACGTCGAATTGTGCGGGTAGCGATGCCCATAAGCCAGCGAGACAACAAAGAAAAAAAGAATGA                                                            <  SRR4302533.4346531/94‑1 (MQ=60)
                                  GTTCAATCATTAAATGCGGTAAATCCGCCTGACGTCGAATTGTGCGGGTAGCGATGCCCATAAGCCAGCGAGACAACAAAGAAAAAAAGAATGA                                                            <  SRR4302533.204161/94‑1 (MQ=60)
                                   TTCAATCATTAAATGCGGTAAATCCGCCTGACGTCGAATTGTGCGGGTAGCGATGCCCATAAGCCAGCGAGACAACAAAGAAAAAAAGAATGAT                                                           <  SRR4302533.5226726/94‑1 (MQ=60)
                                   TTCAATCATTAAATGCGGTAAATCCGCCTGACGTCGAATTGTGCGGGTAGCGATGCCCATAAGCCAGCGAGACAACAAAGAAAAAAAGAATGAT                                                           >  SRR4302533.3970835/1‑94 (MQ=60)
                                     CAATCATTAAATGCGGTAAATCCGCCTGACGTCGAATTGTGCGGGTAGCGATGCCCATAAGCCAGCGAGACAACAAAGAAAAAAAGAATGATCA                                                         >  SRR4302533.4774924/1‑94 (MQ=60)
                                     CAATCATTAAATGCGGTAAATCCGCCTGACGTCGAATTGTGCGGGTAGCGATGCCCATAAGCCAGCGAGACAACAAAGAAAAAAAGAATGATCA                                                         >  SRR4302533.5423194/1‑94 (MQ=60)
                                     CAATCATTAAATGCGGTAAATCCGCCTGACGTCGAATTGTGCGGGTAGCGATGCCCATAAGCCAGCGAGACAACAAAGAAAAAAAGAATGATCA                                                         >  SRR4302533.4681039/1‑94 (MQ=60)
                                     CAATCATTAAATGCGGTAAATCCGCCTGACGTCGAATTGTGCGGGTAGCGATGCCCATAAGCCAGCGAGACAACAAAGAAAAAAAGAATGATCA                                                         <  SRR4302533.4620559/94‑1 (MQ=60)
                                      AATCATTAAATGCGGTAAATCCGCCTGACGTCGAATTGTGCGGGTAGCGATGCCCATAAGCCAGCGAGACAACAAAGAAAAAAAGAATGATCAG                                                        <  SRR4302533.5145222/94‑1 (MQ=60)
                                      AATCATTAAATGCGGTAAATCCGCCTGACGTCGAATTGTGCGGGTAGCGATGCCCATAAGCCAGCGAGACAACAAAGAAAAAAAGAATGATCAG                                                        <  SRR4302533.4445555/94‑1 (MQ=60)
                                       ATCATTAAATGCGGTAAATCCGCCTGACGTCGAATTGTGCGGGTAGCGATGCCCATAAGCCAGCGAGACAACAAAGAAAAAAAGAATGATCAGT                                                       <  SRR4302533.1406937/94‑1 (MQ=60)
                                        TCATTAAATGCGGTAAATCCGCCTGACGTCGAATTGTGCGGGTAGCGATGCCCATAAGCCAGCGAGACAACAAAGAAAAAAAGAATGATCAGTG                                                      <  SRR4302533.4725988/94‑1 (MQ=60)
                                         CATTAAATGCGGTAAATCCGCCTGACGTCGAATTGTGCGGGTAGCGATGCCCATAAGCCAGCGAGACAACAAAGAAAAAAAGAATGATCAGTGG                                                     <  SRR4302533.5869866/94‑1 (MQ=60)
                                           TTAAATGCGGTAAATCCGCCTGACGTCGAATTGTGCGGGTAGCGATGCCCATAAGCCAGCGAGAGAACAAAGAAAAAAAGAATGATCAGTGGCA                                                   >  SRR4302533.430694/1‑94 (MQ=60)
                                           TTAAATGCGGTAAATCCGCCTGACGTCGAATTGTGCGGGTAGCGATGCCCATAAGCCAGCGAGACAACAAAGAAAAAAAGAATGATCAGTGGCA                                                   <  SRR4302533.4262900/94‑1 (MQ=60)
                                           TTAAATGCGGTAAATCCGCCTGACGTCGAATTGTGCGGGTAGCGATGCCCATAAGCCAGAGAGACAACAAAGAAAAAAAGAATAATCAGTGGCA                                                   >  SRR4302533.3107536/1‑94 (MQ=60)
                                               ATGCGGTAAATCCGCCTGACGTCGAATTGTGCGGGTAGCGATGCCCATAAGCCAGCGAGACAACAAAGAAAAAAAGAATGATCAGTGGCACGAT                                               >  SRR4302533.4424612/1‑94 (MQ=60)
                                               ATGCGGTAAATCCGCCTGACGTCGAATTGTGCGGGTAGCGATGCCCATAAGCCAGCGAGACAACAAAGAAAAAAAGAATGATCAGTGGCACGAT                                               >  SRR4302533.3040374/1‑94 (MQ=60)
                                                TGCGGTAAATCCGCCTGACGTCGAATTGTGCGGGTAGCGATGCCCATAAGCCAGCGAGACAACAAAGAAAAAAAGAATGATCAGTGGCACGATA                                              <  SRR4302533.313832/94‑1 (MQ=60)
                                                 GCGGTAAATCCGCCTGACGTCGAATTGTGCGGGTAGCGATGCCCATAAGCCAGCGAGACAACAAAGAAAAAAAGAATGATCAGTGGCACGATAC                                             <  SRR4302533.5031558/94‑1 (MQ=60)
                                                  CGGTAAATCCGCCTGACGTCGAATTGTGCGGGTAGCGATGCCCATAAGCCAGCGAGACAACAAAGAAAAAAAGAATGATCAGTGGCACGATACC                                            >  SRR4302533.3298130/1‑94 (MQ=60)
                                                   gttaaaccggctTGACGTCGAATGGTGCGGGTAGCGATGCCCATAAGCCAGCGAGACAACAAAGAAAAAAAGAATGATCAGTGGCACGATACCT                                           <  SRR4302533.5998688/82‑1 (MQ=60)
                                                   GGTAAATCTGCCTGACGTCGAATTGTGCGGGTAGCGATGCCCATAAGCCAGCGTGACAACAAAGAAAAAAAGAATGATCAGTGGCACGATACCT                                           >  SRR4302533.3343813/1‑94 (MQ=60)
                                                   GGTAAATCCGCCTGACGTCGAATTGTGCGGGTAGCGATGCCCATAAGCCAGCGAGACAACAAAGAAAAAAAGAATGATCAGTGGCACGATACCT                                           <  SRR4302533.219366/94‑1 (MQ=60)
                                                   GGTAAATCCGCCTGACGTCGAATTGTGCGGGTAGCGATGCCCATAAGCCAGCGAGACAACAAAGAAAAAAAGAATGATCAGTGGCACGATACCT                                           >  SRR4302533.2137009/1‑94 (MQ=60)
                                                   GGTAAATCCGCCTGACGTCGAATTGTGCGGGTAGCGATGCCCATAAGCCAGCGAGACAACAAAGAAAAAAAGAATGATCAGTGGCACGATACCT                                           <  SRR4302533.752657/94‑1 (MQ=60)
                                                     TAAATCCGCGTGACGTCGAATTGTGCGGGTAGCGATGCCCATAAGCCAGCGAGACAACAAAGAAAAAAAGAATGATCAGTGGCACGATACCTTT                                         <  SRR4302533.992285/94‑1 (MQ=60)
                                                      AAATCCGCCTGACGTCGAATTGTGCGGGTAGCGATGCCCATAAGCCAGCGAGACAACAAAGAAAAAAAGAATGATCAGTGGCACGATACCTTTA                                        <  SRR4302533.4610514/94‑1 (MQ=60)
                                                          CCGCCTGACGTCGAATTGTGCGGGTAGCGATGCCCATAAGCCAGCGAGACAACAAAGAAAAAAAGAATGATCAGTGGCACGATACCTTTAATAA                                    >  SRR4302533.76594/1‑94 (MQ=60)
                                                          CCGCCTGACGTCGAATTGTGCGGGTAGCGATGCCCATAAGCCAGCGAGACAACAAAGAAAAAAAGAATGATCAGTGGCACGATACCTTTAATAA                                    <  SRR4302533.4445897/94‑1 (MQ=60)
                                                           CGCCTGACGTCGAATTGTGCGGGTAGCGATGCCCATAAGCCAGCGAGACAACAAAGAAAAAAAGAATGATCAGTGGCACGATACCTTTAATAAA                                   >  SRR4302533.4319837/1‑94 (MQ=60)
                                                           CGCCTGACGTCGAATTGTGCGGGTAGCGATGCCCATAAGCCAGCGAGACAACAAAGAAAAAAAGAATGATCAGTGGCACGATACATTTAATAAA                                   >  SRR4302533.3432522/1‑94 (MQ=60)
                                                            GCCTGACGTCGAATTGTGCGGGTAGCGATGCCCATAAGCCAGCGAGACAACAAAGAAAAAAAGAATGATCAGTGGCACGATACCTTTAATAAAG                                  >  SRR4302533.946399/1‑94 (MQ=60)
                                                             CCTGACGTCGAATTGTGCGGGTAGCGATGCCCATAAGCCAGCGAGACAACAAAGAAAAAAAGAATGATCAGTGGCACGATACCTTTAATAAAGG                                 <  SRR4302533.6310075/94‑1 (MQ=60)
                                                             CCTGACGTCGAATTGTGCGGGTAGCGATGCCCATAAGCCAGCGAGACAACAAAGAAAAAAAGAATGATCAGTGGCACGATACCTTTAATAAAGG                                 <  SRR4302533.591503/94‑1 (MQ=60)
                                                              CTGACGTCGAATTGTGCGGGTAGCGATGCCCATAAGCCAGCGAGACAACAAAGAAAAAAAGAATGATCAGTGGCACGATACCTTTAATAAAGGG                                <  SRR4302533.235767/94‑1 (MQ=60)
                                                              CTGACGTCGAATTGTGCGGGTAGCGATGCCCATAAGCCAGCGAGACAACAAAGAAAAAAAGAATGATCAGTGGCACGATACCTTTAATAAAGGG                                <  SRR4302533.1027456/94‑1 (MQ=60)
                                                              CTGACGTCGAATTGTGCGGGTAGCGATGCCCATAAGCCAGCGAGACAACAAAGAAAAAAAGAATGATCAGTGGCACGATACCTTTAATAAAGGG                                >  SRR4302533.1893530/1‑94 (MQ=60)
                                                               TGACGTCGAATTGTGCGGGTAGCGATGCCCATAAGCCAGCGAGACAACAAAGAAAAAAAGAATGATCAGTGGCACGATACCTTTAATAAAGGGT                               <  SRR4302533.5015585/94‑1 (MQ=60)
                                                               TGACGTCGAATTGTGCGGGTAGCGATGCCCATAAGCCAGCGAGACAACAAAGAAAAAAAGAATGATCAGTGGCACGATACCTTTAATAAAGGGT                               <  SRR4302533.962320/94‑1 (MQ=60)
                                                               TGACGTCGAATTGTGCGGGTAGCGATGCCCATAAGCCAGCGAGACAACAAAGAAAAAAAGAATGATCAGTGGCACGATACCTTTAATAAAGGGT                               <  SRR4302533.4415069/94‑1 (MQ=60)
                                                                GACGTCGAATTGTGCGGGTAGCGATGCCCATAAGCCAGCGAGACAACAAAGAAAAAAAGAATGATCAGTGGCACGATACCTTTAATAAAGGGTG                              >  SRR4302533.5329268/1‑94 (MQ=60)
                                                                 ACGTCGAATTGTGCGGGTAGCGATGCCCATAAGCCAGCGAGACAACAAAGAAAAAAAGAATGATCAGTGGCACGATACCTTTAATAAAGGGTGA                             <  SRR4302533.6172661/94‑1 (MQ=60)
                                                                   GTCGAATTGTGCGGGTAGCGATGCCCATAAGCCAGCGAGACAACAAAGAAAAAAAGAATGATCAGTGGCACGATACCTTTAATAAAGGGTGATG                           <  SRR4302533.4282767/94‑1 (MQ=60)
                                                                        ATTGTGCGGGTAGCGATGCCCATAAGCCAGCGAGACAACAAAGAAAAAAAGAATGATCAGTGGCACGATACCTTTAATAAAGGGTGATGGCATC                      <  SRR4302533.1733788/94‑1 (MQ=60)
                                                                         TTGTGCGGGTAGCGATGCCCATAAGCCAGCGAGACAACAAAGAAAAAAAGAATGATCAGTGGCACGATACCTTTAATAAAGGGTGATGGCATCA                     <  SRR4302533.878220/94‑1 (MQ=60)
                                                                          TGTGCGGGTAGCGATGCCCATAAGCCAGCGAGACAACAAAGAAAAAAAGAATGATCAGTGGCACGATACCTTTAATACAGGGTGATGGCATCAC                    >  SRR4302533.3885630/1‑94 (MQ=60)
                                                                           GTGCGGGTAGCGATGCCCATAAGCCAGCGAGACAACAAAGAAAAAAAGAATGATCAGTGGCACGATACCTTTAATAAAGGGTGATGGCATCACG                   <  SRR4302533.198825/94‑1 (MQ=60)
                                                                            TGCGGGTAGCGATGCCCATAAGCCAGCGAGACAACAAAGAAAAAAAGAATGATCAGTGGCACGATACCTTTAATAAAGGGTGATGGCATCACGG                  <  SRR4302533.4367383/94‑1 (MQ=60)
                                                                             GCGGGTAGCGATGCCCATAAGCCAGCGAGACAACAAAGAAAAAAAGAATGATCAGTGGCACGATACCTTTAATAAAGGGTGATGGCATCACGGT                 <  SRR4302533.5851586/94‑1 (MQ=60)
                                                                              CGGGTAGCGATGCCCATAAGCCAGCGAGACAACAAAGAAAAAAAGAATGATCAGTGGCACGATACCTTTAATAAAGGGTGATGGCATCACGGTG                <  SRR4302533.1819312/94‑1 (MQ=60)
                                                                               GGGTAGCGATGCCCATAAGCCAGCGAGACAACAAAGAAAAAAAGAATGATCAGTGGCACGATACCTTTAATAAAGGGTGATGGCATCACGGTGT               <  SRR4302533.891949/94‑1 (MQ=60)
                                                                               GGGTAGCGATGCCCATAAGCCAGCGAGACAACAAAGAAAAAAAGAATGATCAGTGGCACGATACCTTTAATAAAGGGTGATGGCATCACGGTGT               <  SRR4302533.237321/94‑1 (MQ=60)
                                                                               GGGTAGCGATGCCCATAAGCCAGCGAGACAACAAAGAAAAAAAGAATGATCAGTGGCACGATACCTTTAATAAAGGGTGATGGCATCACGGTGT               <  SRR4302533.1999434/94‑1 (MQ=60)
                                                                                GGTAGCGATGCCCATAAGCCAGCGAGACAACAAAGAAAAAAAGAATGATCAGTGGCACGATACCTTTAATAAAGGGTGATGGCATCACGGTGTG              <  SRR4302533.1422163/94‑1 (MQ=60)
                                                                                  TAGCGATGCCCATAAGCCAGCGAGACAACAAAGAAAAAAAGAATGATCAGTGGCACGATACCTTTAATAAAGGGTGATGGCATCACGGTGTGAT            <  SRR4302533.1155274/94‑1 (MQ=60)
                                                                                   AGCGATGCCCATAAGCCAGCGAGACAACAAAGAAAAAAAGAATGATCAGTGGCACGATACCTTTAATAAAGGGTGATGGCATCACGGTGTGATT           >  SRR4302533.3436739/1‑94 (MQ=60)
                                                                                       ATGCCCATAAGCCAGCGAGACAACAAAGAAAAAAAGAATGATCAGTGGCACGATACCTTTAATAAAGGGTGATGGCATCACGGTGTGATTAATC       <  SRR4302533.4057908/94‑1 (MQ=60)
                                                                                        tgcCCATAAGCCAGCGAGACAACAAAGAAAAAAAGAATGATCAGTGGCACGATACCTTTAATAAAGGGTGATGGCATCACGGTGTGATTAATCG      <  SRR4302533.2233811/91‑1 (MQ=60)
                                                                                        agcCCATAAGCCAGCGAGACAACAAAGAAAAAAAGAATGATCAGTGGCACGATACCTTTAATAAAGGGTGATGGCATCACGGTGTGATTAATCG      <  SRR4302533.5172785/91‑1 (MQ=60)
                                                                                           CCATAAGCCAGCGAGACAACAAAGAAAAAAAGAATGATCAGTGGCACGATACCTTTAATAAAGGGTGATGGCATCACGGTGTGATTAATCGGAT   >  SRR4302533.55677/1‑94 (MQ=60)
                                                                                            CATAAGCCAGCGAGACAACAAAGAAAAAAAGAATGATCAGTGGCACGATACCTTTAATAAAGGGTGATGGCATCACGGTGTGATTAATCGGATC  <  SRR4302533.4728595/94‑1 (MQ=60)
                                                                                            |                                                                                             
ACCATCACGATAAATCCCGCCATCTCTTTCATCGGTTCAATCATTAAATGCGGTAAATCCGCCTGACGTCGAATTGTGCGGGTAGCGATG‑CCATAAGCCAGCGAGACAACAAAGAAAAAAAGAATGATCAGTGGCACGATACCTTTAATAAAGGGTGATGGCATCACGGTGTGATTAATCGGATC  >  NC_000913/1398749‑1398933

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑