Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1624	250.6	12620048	98.0%	12367647	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,747,963	T→C	H137R (CAC→CGC)	ydhU ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,747,963	0	T	C	99.5%	624.5 / NA	194	H137R (CAC→CGC)	ydhU	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (112/81); minor base A (1/0); total (113/81)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 209 total aligned reads displayed.

GGATGTTCTTCCCCCTGCATAATGCCAAACAAATAAAATCGCGTTTGTTTTGCCGCTCGTTCCAGCCACCCCTGACGACGAATGCGATAGTGGTGACCATTATCCCCAACGGCATTGATCAGCACAAAGCCGAGCCAGCATGCCAGTAACAAAAATCCGCAAACTTCATGCACCGCAACC  >  NC_000913/1747873‑1748052

 gATGTTCTTCCCCCTGCATAATGCCAAACAAATAAAATCGCGTTTGTTTTGCCGCTCGTTCCAGCCACCCCTGACGACGAATGCGATAgcggt                                                                                        <  1:913814/93‑1 (MQ=255)

  aTGTTCTTCCCCCTGCATAATGCCAAACAAATAAAATCGCGTTTGTTTTGCCGCTCGTTCCAGCCACCCCTGACGACGAATGCGATAgcggtg                                                                                       >  2:4676258/1‑93 (MQ=255)

   tGTTCTTCCCCCTGCATAATGCCAAACAAATAAAATCGCGTTTGTTTTGCCGCTCGTTCCAGCCACCCCTGACGACGAATGCGATAGCGgaga                                                                                      >  1:2365828/1‑93 (MQ=255)

   tGTTCTTCCCCCTGCATAATGCCAAACAAATAAAATCGCGTTTGTTTTGCCGCTCGTTCCAGCCACCCCTGACGACGAATGCGATAGCGGTGa                                                                                      <  1:1170070/93‑1 (MQ=255)

    gTTCTTCCCCCTGCATAATGCCAAACAAATAAAATCGCGTTTGTTTTGCCGCTCGTTCCAGCCACCCCTGACGACGAATGCGATAGCGGTGAc                                                                                     >  1:5276570/1‑93 (MQ=255)

    gTTCTTCCCCCTGCATAATGCCAAACAAATAAAATCGCGTTTGTTTTGCCGCTCGTTCCAGCCACCCCTGACGACGAATGCGATAGCGGTGAc                                                                                     <  2:1300504/93‑1 (MQ=255)

     ttcttcCCCCTGCATAATGCCAAACAAATAAAATCGCGTTTGTTTTGCCGCTCGTTCCAGCCACCCCTGACGACGAATGCGATAGCGGTGAcc                                                                                    <  2:5047801/93‑1 (MQ=255)

     ttcttcCCCCTGCATAATGCCAAACAAATAAAATCGCGTTTGTTTTGCCGCTCGTTCCAGCCACCCCTGACGACGAATGCGATAGCGGTGAcc                                                                                    <  1:2848526/93‑1 (MQ=255)

     ttcttcCCCCTGCATAATGCCAAACAAATAAAATCGCGTTTGTTTTGCCGCTCGTTCCAGCCACCCCTGACGACGAATGCGATAGCGGTGAca                                                                                    >  1:1986128/1‑92 (MQ=255)

         tcCCCCTGCATAATGCCAAACAAATAAAATCGCGTTTGTTTTGCCGCTCGTTCCAGCCACCCCTGACGACGAATGCGATAGCGGTGACCATTa                                                                                >  2:699219/1‑93 (MQ=255)

         tcCCCCTGCATAATGCCAAACAAATAAAATCGCGTTTGTTTTGCCGCTCGTTCCAGCCACCCCTGACGACGAATGCGATAGCGGTGACCATTa                                                                                >  2:265054/1‑93 (MQ=255)

         tcCCCCTGCATAATGCCAAACAAATAAAATCGCGTTTGTTTTGCCGCTCGTTCCAGCCACCCCTGACGACGAATGCGATAGCGGTGACCATTa                                                                                <  2:248418/93‑1 (MQ=255)

          cccccTGCATAATGCCAAACAAATAAAATCGCGTTTGTTTTGCCGCTCGTTCCAGCCACCCCTGACGACGAATGCGATAGCGGTGACCATTAt                                                                               <  1:5455030/93‑1 (MQ=255)

           ccccTGCATAATGCCAAACAAATAAAATCGCGTTTGTTTTGCCGCTCGTTCCAGCCACCCCTGACGACGAATGCGATAGCGGTGACCATTATc                                                                              >  1:1873720/1‑93 (MQ=255)

           ccccTGCATAATGCCAAACAAATAAAATCGCGTTTGTTTTGCCGCTCGTTCCAGCCACCCCTGACGACGAATGCGATAGCGGTGACCATTATc                                                                              >  1:5131664/1‑93 (MQ=255)

           ccccTGCATAATGCCAAACAAATAAAATCGCGTTTGTTTTGCCGCTCGTTCCAGCCACCCCTGACGACGAATGCGATAGAGGTGACCATTATc                                                                              >  1:2689651/1‑93 (MQ=255)

             ccatcATGATGCAAAACAAATTAAAGCCCGTTTGCTTTGCCGCTCGTTCCAGCCACCCCTGACGACGAATGCGATAGCGGTGACCATTATccc                                                                            <  2:1832087/89‑1 (MQ=255)

             ccTGCATAATGCCAAACAAATAAAATCGCGTTTGTTTTGCCGCTCGTTCCAGCCACCCCTGACGACGAATGCGATAGCGGTGACCATTATccc                                                                            >  1:273489/1‑93 (MQ=255)

              cTGCATAATGCCAAACAAATAAAATCGCGTTTGTTTTGCCGCTCGTTCCAGCCACCCCTGACGACGAATGCGATAGCGGTGACCATTATcccc                                                                           >  1:4556015/1‑93 (MQ=255)

               tGCATAATGCCAAACAAATAAAATCGCGTTTGTTTTGCCGCTCGTTCCAGCCACCCCTGACGACGAATGCGATAGCGGTGACCATTATCCCCa                                                                          >  2:5794104/1‑93 (MQ=255)

                gCATAATGCCAAACAAATAAAATCGCGTTTGTTTTGCCGCTCGTTCCAGCCACCCCTGACGACGAATGCGATAGCGGTGACCATTATCCCCaa                                                                         >  2:3932661/1‑93 (MQ=255)

                gCATAATGCCAAACAAATAAAATCGCGTTTGTTTTGCCGCTCGTTCCAGCCACCCCTGACGACGAATGCGATAGCGGTGACCATTATCCCCaa                                                                         >  2:4090360/1‑93 (MQ=255)

                gCATAATGCCAAACAAATAAAATCGCGTTTGTTTTGCCGCTCGTTCCAGCCACCCCTGACGACGAATGCGATAGCGGTGACCATTATCCCCaa                                                                         >  1:352009/1‑93 (MQ=255)

                gCATAATGCCAAACAAATAAAATCGCGTTTGTTTTGCCGCTCGTTCCAGCCACCCCTGACGACGAATGCGATAGCGGTGACCATTATCCCCaa                                                                         <  2:5132296/93‑1 (MQ=255)

                 cATAATGCCAAACAAATAAAATCGCGTTTGTTTTGCCGCTCGTTCCAGCCACCCCTGACGACGAATGCGATAGCGGTGACCATTATCCCCAAc                                                                        <  2:3101450/93‑1 (MQ=255)

                 cATAATGCCAAACAAATAAAATCGCGTTTGTTTTGCCGCTCGTTCCAGCCACCCCTGACGACGAATGCGATAGCGGTGACCATTATCCCCAAc                                                                        <  1:6194983/93‑1 (MQ=255)

                 cATAATGCCAAACAAATAAAATCGCGTTTGTTTTGCCGCTCGTTCCAGCCACCCCTGACGACGAATGCGATAGCGGTGACCATTATCCCCAAc                                                                        <  2:5325964/93‑1 (MQ=255)

                 cATAATGCCAAACAAATAAAATCGCGTTTGTTTTGCCGCTCGTTCCAGCCACCCCTGACGACGAATGCGATAGCGGTGACCATTATCCCCAAc                                                                        <  1:491789/93‑1 (MQ=255)

                  aTAATGCCAAACAAATAAAATCGCGTTTGTTTTGCCGCTCGTTCCAGCCACCCCTGACGACGAATGCGATAGCGGTGACCATTATCCCCAACg                                                                       <  1:2436816/93‑1 (MQ=255)

                  aTAATGCCAAACAAATAAAATCGCGTTTGTTTTGCCGCTCGTTCCAGCCACCCCTGACGACGAATGCGATAGCGGTGACCATTATCCCCAACg                                                                       >  2:1665948/1‑93 (MQ=255)

                  aTAATGCCAAACAAATAAAATCGCGTTTGTTTTGCCGCTCGTTCCAGCCACCCCTGACGACGAATGCGATAGCGGTGACCATTATCCCCAACg                                                                       >  1:203014/1‑93 (MQ=255)

                      tGCCAAACAAATAAAATCGCGTTTGTTTTGCCGCTCGTTCCAGCCACCCCTGACGACGAATGCGATAGCGGTGACCATTATCCCCAACGGCAt                                                                   >  1:1766731/1‑93 (MQ=255)

                      agCCAAACAAATAAAATCGCGTTTGTTTTGCCGCTCGTTCCAGCCACCCCTGACGACGAATGCGATAGCGGTGACCATTATCCCCAACGGCAt                                                                   <  1:4477696/92‑1 (MQ=255)

                          aaacaaaTAAAATCGCGTTTGTTTTGCCGCTCGTTCCAGCCACCCCTGACGACGAATGCGATAGCGGTGACCATTATCCCCAACGGCATTGAt                                                               >  2:2681582/1‑93 (MQ=255)

                          aaacaaaTAAAATCGCGTTTGTTTTGCCGCTCGTTCCAGCCACCCCTGACGACGAATGCGATAGCGGTGACCATTATCCCCAACGGCATTGAt                                                               <  1:569823/93‑1 (MQ=255)

                          aaacaaaTAAAATCGCGTTTGTTTTGCCGCTCGTTCCAGCCACCCCTGACGACGAATGCGATAGCGGTGACCATTATCCCCAACGGCATTGAt                                                               >  1:2774712/1‑93 (MQ=255)

                          aaacaaaTAAAATCGCGTTTGTTTTGCCGCTCGTTCCAGCCACCCCTGACGACGAATGCGATAGCGGTGACCATTATCCCCAACGGCATTGAt                                                               >  1:4072467/1‑93 (MQ=255)

                           aacaaaTAAAATCGCGTTTGTTTTGCCGCTCGTCCCAGCCACCCCTGACGACGAATGCGATAGCGGTGACCATTATCCCCAACGGCATTGATc                                                              <  1:4611854/93‑1 (MQ=255)

                            acaaaTAAAATCGCGTTTGTTTTGCCGCTCGTTCCAGCCACCCCTGACGACGAATGCGATAGCGGTGACCATTATCCCCAACGGCATTGATCa                                                             >  2:2534469/1‑93 (MQ=255)

                            acaaaTAAAATCGCGTTTGTTTTGCCGCTCGTTCCAGCCACCCCTGACGACGAATGCGATAGCGGTGACCATTATCCCCAACGGCATTGATCa                                                             <  1:1025610/93‑1 (MQ=255)

                            acaaaTAAAATCGCGTTTGTTTTGCCGCTCGTTCCAGCCACCCCTGACGACGAATGCGATAGCGGTGACCATTATCCCCAACGGCATTGATCa                                                             >  1:5215735/1‑93 (MQ=255)

                            acaaaTAAAATCGCGTTTGTTTTGCCGCTCGTTCCAGCCACCCCTGACGACGAATGCGATAGCGGTGACCATTATCCCCAACGGCATTGATCa                                                             >  1:5612523/1‑93 (MQ=255)

                             caaaTAAAATCGCGTTTGTTTTGCCGCTCGTTCCAGCCACCCCTGACGACGAATGCGATAGCGGTGACCATTATCCCCAACGGCATTGATCAg                                                            <  2:1197255/93‑1 (MQ=255)

                              aaaTAAAATCGCGTTTGTTTTGCCGCTCGTTCCAGCCACCCCTGACGACGAATGCGATAGCGGTGACCATTATCCCCAACGGCATTGATCAGc                                                           >  1:1618458/1‑93 (MQ=255)

                              aaaTAAAATCGCGTTTGTTTTGCCGCTCGGTCCAGCCACCCCTGACGACGAATGCGATAGCGGTGACCATTATCCCCAACGGCATTGATCAGc                                                           >  1:5640952/1‑93 (MQ=255)

                               aaTAAAATCGCGTTTGTTTTGCCGCTCGTTCCAGCCACCCCTGACGACGAATGCGATAGCGGTGACCATTATCCCCAACGGCATTGATCAGca                                                          <  1:1523740/93‑1 (MQ=255)

                                 tAAAATCGCGTTTGTTTTGCCGCTCGTTCCAGCCACCCCTGACGACGAATGCGATAGCGGTGACCATTATCCCCAACGGCATTGATCAGcaca                                                        >  1:298276/1‑93 (MQ=255)

                                      tCGCGTTTGTTTTGCCGCTCGTTCCAGCCACCCCTGACGACGAATGCGATAGCGGTGACCATTATCCCCAACGGCATTGATCAGCACAAAGcc                                                   <  2:169175/93‑1 (MQ=255)

                                        gcgTTTGTTTTGCCGCTCGTTCCAGCCACCCCTGACGACGAATGCGATAGCGGTGACCATTATCCCCAACGGCATTGATCAGCACAAAGCCGa                                                 <  1:5019103/93‑1 (MQ=255)

                                         cgTTTGTTTTGCCGCTCGTTCCAGCCACCTCTGACGACGAATGTGATACCGGTGACCATTATCCCCAACGGCATTGATCAGCACAAAGCCGAg                                                >  2:6017232/1‑93 (MQ=255)

                                         cgTTTGTTTTGCCGCTCGTTCCAGCCACCCCTGACGACGAATGCGATAGCGGTGACCATTATCCCTAACGGCATTGATCAGCACAAAGCCGAg                                                >  2:2145484/1‑93 (MQ=255)

                                         cgTTTGTTTTGCCGCTCGTTCCAGCCACCCCTGACGACGAATGCGATAGCGGTGACCATTATCCCCAACGGCATTGATCAGCACAAAGCCGAg                                                <  1:5082222/93‑1 (MQ=255)

                                           tttgtttTGCCGCTCGTTCCAGCCACCCCTGACGACGAATGCGATAGCGGTGACCATTATCCCCAACGGCATTGATCAGCACAAAGCCGAGcc                                              >  2:4808246/1‑93 (MQ=255)

                                           tttgtttTGCCGCTCGTTCCAGCCACCCCTGACGACGAATGCGATAGCGGTGACCATTATCCCCAACGGCATTGATCAGCACAAAGCCGAGcc                                              >  1:2122384/1‑93 (MQ=255)

                                           tttgtttTGCCGCTCGTTCCAGCCACCCCTGACGACGAATGCGATAGCGGTGACCATTATCCCCAACGGCATTGATCAGCACAAAGCCGAGcc                                              >  2:3720297/1‑93 (MQ=255)

                                              gtttTGCCGCTCGTTCCAGCCACCCCTGACGACGAATGCGATAGCGGTGACCATTATCCCCAACGGCATTGATCAGCACAAAGCCGAGCCAGc                                           >  1:4952731/1‑93 (MQ=255)

                                               ttttGCCGCTCGTTCCAGCCACCCCTGACGACGAATGCGATAGCGGTGACCATTATCCCCAACGGCATTGATCAGCACAAAGCCGAGCCAGCa                                          >  2:755778/1‑93 (MQ=255)

                                               ttttGCCGCTCGTTCCAGCCACCCCTGACGACGAATGCGATAGCGGTGACCATTATCCCCAACGGCATTGATCAGCACAAAGCCGAGCCAGCa                                          <  1:1238820/93‑1 (MQ=255)

                                                 ttGCCGCTCGTTCCAGCCACCCCTGACGACGAATGCGATAGCGGTGACCATTATCCCCAACGGCATTGATCAGCACAAAGCCGAGCCAGCATg                                        <  2:5288083/93‑1 (MQ=255)

                                                   gCCGCTCGTTCCAGCCACCCCTGACGACGAATGCGATAGCGGTGACCATTATCCCCAACGGCATTGATCAGCACAAAGCCGAGCCAGCATGcc                                      >  1:4680464/1‑93 (MQ=255)

                                                   gCCGCTCGTTCCAGCCACCCCTGACGACGAATGCGATAGCGGTGACCATTATCCCCAACGGCATTGATCAGCACAAAGCCGAGCCAGCATGcc                                      <  2:1553464/93‑1 (MQ=255)

                                                    ccGCTCGTTCCAGCCACCCCTGACGACGAATGCGATAGCGGTGACCATTATCCCCAACGGCATTGATCAGCACAAAGCCGAGCCAGCATGCCa                                     <  2:5871162/93‑1 (MQ=255)

                                                     cGCTCGTTCCAGCCACCCCTGACGACGAATGCGATAGCGGTGACCATTATCCCCAACGGCATTGATCAGCACAAAGCCGAGCCAGCATGCCAg                                    <  1:5354869/93‑1 (MQ=255)

                                                     cGCTCGTTCCAGCCACCCCTGACGACGAATGCGATAGCGGTGACCATTATCCCCAACGGCATTGATCAGCACAAAGCCGAGCCAGCATGCCAg                                    <  1:5038164/93‑1 (MQ=255)

                                                     cGCTCGTTCCAGCCACCCCTGACGACGAATGCGATAGCGGTGACCATTATCCCCAACGGCATTGATCAGCACAAAGCCGAGCCAGCATGCCAg                                    >  1:685577/1‑93 (MQ=255)

                                                     cGCTCGTTCCAGCCACCCCTGACGACGAATGCGATAGCGGTGACCATTATCCCCAACGGCATTGATCAGCACAAAGCCGAGCCAGCATGCCAg                                    >  2:5628506/1‑93 (MQ=255)

                                                      gCTCGTTCCAGCCACCCCTGACGACGAATGCGATAGCGGTGACCATTATCCCCAACGGCATTGATCAGCACAAAGCCGAGCCAGCATGCCAGt                                   >  2:5691280/1‑93 (MQ=255)

                                                         cGTTCCAGCCACCCCTGACGACGAATGCGATAGCGGTGACCATTATCCCCAACGGCATTGATCAGCACAAAGCCGAGCCAGCATGCCAGTAAc                                >  1:3583850/1‑93 (MQ=255)

                                                           ttCCAGCCACCCCTGACGACGAATGCGATAGCGGTGACCATTATCCCCAACGGCATTGATCAGCACAAAGCCGAGCCAGCATGCCAGTAACaa                              >  1:3879578/1‑93 (MQ=255)

                                                             ccAGCCACCCCTGACGACGAATGCGATAGCGGTGACCATTATCCCCAACGGCATTGATCAGCACAAAGCCGAGCCAGCATGCCAGTAACaaaa                            >  2:2275621/1‑93 (MQ=255)

                                                              cAGCCACCCCTGACGACGAATGCGATAGCGGTGACCATTATCCCCAACGGCATTGATCAGCACAAAGCCGAGCCAGCATGCCAGTAACaaaaa                           <  2:2987075/93‑1 (MQ=255)

                                                               aGCCACCCCTGACGACGAATGCGATAGCGGTGACCATTATCCCCAACGGCATTGATCAGCACAAAGCCGAGCCAGCATGCCAGTAACAAAAAt                          >  1:2494898/1‑93 (MQ=255)

                                                               aGCCACCCCTGACGACGAATGCGATAGCGGTGACCATTATCCCCAACGGCATTGATCAGCACAAAGCCGAGCCAGCATGCCAGTAACAAAAAt                          <  1:3299941/93‑1 (MQ=255)

                                                                gCCACCCCTGACGACGAATGCGATAGCGGTGACCATTATCCCCAACGGCATTGATCAGCACAAAGCCGAGCCAGCATGCCAGTAACAAAAATc                         >  1:1366842/1‑93 (MQ=255)

                                                                  cACCCCTGACGACGAATGCGATAGCGGTGACCATTATCCCCAACGGCATTGATCAGCACAAAGCCGAGCCAGCATGCCAGTAACAAAAATCCg                       >  1:5934432/1‑93 (MQ=255)

                                                                    ccccTGACGACGAATGCGATAGCGGTGACCATTATCCCCAACGGCATTGATCAGCACAAAGCCGAGCCAGCATGCCAGTAACAAAAATCCGCa                     <  2:545461/93‑1 (MQ=255)

                                                                    ccccTGACGACGAATGCGATAGCGGTGACCATTATCCCCAACGGCATTGATCAGCACAAAGCCGAGCCAGCATGCCAGTAACAAAAATCCGCa                     >  2:815821/1‑93 (MQ=255)

                                                                    ccccTGACGACGAATGCGATAGCGGTGACCATTATCCCCAACGGCATTGATCAGCACAAAGCCGAGCCAGCATGCCAGTAACAAAAATCCGCa                     >  1:368119/1‑93 (MQ=255)

                                                                     cccTGACGACGAATGCGATAGCGGTGACCATTATCCCCAACGGCATTGATCAGCACAAAGCCGAGCCAGCATGCCAGTAACAAAAATCCGCaa                    <  2:4963856/93‑1 (MQ=255)

                                                                      ccTGACGACGAATGCGATAGCGGTGACCATTATCCCCAACGGCATTGATCAGCACAAAGCCGAGCCAGCATGCCAGTAACAAAAATCCGCaaa                   >  1:5178063/1‑93 (MQ=255)

                                                                        tGACGACGAATGCGATAGCGGTGACCATTATCCCCAACGGCATTGATCAGCACAAAGCCGAGCCAGCATGCCAGTAACAAAAATCCGCAAACt                 >  1:147328/1‑93 (MQ=255)

                                                                          acgacgAATGCGATAGCGGTGACCATTATCCCCAACGGCATTGATCAGCACAAAGCCGAGCCAGCATGCCAGTAACAAAAATCCGCAAACTTc               <  1:2314065/93‑1 (MQ=255)

                                                                           cgacgaATGCGATAGCGGTGACCATTATCCCCAACGGCATTGATCAGCACAAAGCCGAGCCAGCATGCCAGTAACAAAAATCCGCAAACTTCa              <  2:14352/93‑1 (MQ=255)

                                                                           cgacgaATGCGATAGCGGTGACCATTATCCCCAACGGCATTGATCAGCACAAAGCCGAGCCAGCATGCCAGTAACAAAAATCCGCAAACTTCa              <  2:6285357/93‑1 (MQ=255)

                                                                            gacgaATGCGATAGCGGTGACCATTATCCCCAACGGCATTGATCAGCACAAAGCCGAGCCAGCATGCCAGTAACAAAAATCCGCAAACTTCAt             >  1:427531/1‑93 (MQ=255)

                                                                            gacgaATGCGATAGCGGTGACCATTATCCCCAACGGCATTGATCAGCACAAAGCCGAGCCAGCATGCCAGTAACAAAAATCCGCAAACTTCAt             <  1:4246057/93‑1 (MQ=255)

                                                                             acgaATGCGATAGCGGTGACCATTATCCCCAACGGCATTGATCAGCACAAAGCCGAGCCAGCATGCCAGTAACAAAAATCCGCAAACTTCATg            <  2:4325107/93‑1 (MQ=255)

                                                                             acgaATGCGATAGCGGTGACCATTATCCCCAACGGCATTGATCAGCACAAAGCCGAGCCAGCATGCCAGTAACAAAAATCCGCAAACTTCATg            <  1:786674/93‑1 (MQ=255)

                                                                             acgaATGCGATAGCGGGGACCATTATCCCCAACGGCATTGATCAGCACAAAGCCGAGCCAGCATGCCAGTAACAAAAATCCGCAAACTTCATg            >  2:2037016/1‑93 (MQ=255)

                                                                              cgaATGCGATAGCGGTGACCATTATCCCCAACGGCATTGATCAGCACAAAGCCGAGCCAGCATGCCAGTAACAAAAATCCGCAAACTTCATGc           <  2:3164061/93‑1 (MQ=255)

                                                                              cgaATGCGATAGCGGTGACCATTATCCCCAACGGCATTGATCAGCACAAAGCCGAGCCAGCATGCCAGTAACAAAAATCCGCAAACTTCATGc           <  2:5519589/93‑1 (MQ=255)

                                                                               gaATGCGATAGCGGTGACCATTATCCCCAACGGCATTGATCAGGACAAAGCCGAGCCAGCATGCCAGTAACAAAAATCCGCAAACTTCATgac          >  2:4534675/1‑91 (MQ=255)

                                                                                aaTGCGATAGCGGTGACCATTATCCCCAACGGCATTGATCAGCACAAAGCCGAGCCAGCATGCCAGTAACAAAAATCCGCAAACTTCATGCAc         >  2:2844768/1‑93 (MQ=255)

                                                                                aaTGCGATAGCGGTGACCATTATCCCCAACGGCATTGATCAGCACAAAGCCGAGCCAGCATGCCAGTAACAAAAATCCGCAAACTTCATGCAc         >  2:5183245/1‑93 (MQ=255)

                                                                                aaTGCGATAGCGGTGACCATTATCCCCAACGGCATTGATCAGCACAAAGCCGAGCCAGCATGCCAGTAACAAAAATCCGCAAACTTCATGCAc         >  1:6279806/1‑93 (MQ=255)

                                                                                 aTGCGATAGCGGTGACCATTATCCCCAACGGCATTGATCAGCACAAAGCCGAGCCAGCATGCCAGTAACAAAAATCCGCAAACTTCATGCAcc        >  1:2587200/1‑93 (MQ=255)

                                                                                 aTGCGATAGCGGTGACCATTATCCCCAACGGCATTGATCAGCACAAAGCCGAGCCAGCATGCCAGTAACAAAAATCCGCAAACTTCATGCAcc        >  1:4211113/1‑93 (MQ=255)

                                                                                  tGCGATAGCGGTGACCATTATCCCCAACGGCATTGATCAGCACAAAGCCGAGCCAGCATGCCAGTAACAAAAATCCGCAAACTTCATGCACCg       >  1:1333284/1‑93 (MQ=255)

                                                                                  tGCGATAGCGGTGACCATTATCCCCAACGGCATTGATCAGCACAAAGCCGAGCCAGCATGCCAGTAACAAAAATCCGCAAACTTCATGCACCg       <  1:3119808/93‑1 (MQ=255)

                                                                                       tAGCGGTGACCGTTATCCCCAACGGCATTGATCAGCACAAAGCCGAGCCAGCATGCCAGTAACAAAAATCCGCAAACTTCATGCACCGCAAcc  <  2:3474952/93‑1 (MQ=255)

GGATGTTCTTCCCCCTGCATAATGCCAAACAAATAAAATCGCGTTTGTTTTGCCGCTCGTTCCAGCCACCCCTGACGACGAATGCGATAGTGGTGACCATTATCCCCAACGGCATTGATCAGCACAAAGCCGAGCCAGCATGCCAGTAACAAAAATCCGCAAACTTCATGCACCGCAACC  >  NC_000913/1747873‑1748052

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

AAAGGATGTTCTTCCCCCTGCATAATGCCAAACAAATAAAATCGCGTTTGTTTTGCCGCTCGTTCCAGCCACCCCTGACGACGAATGCGATAGTGGTGACCATTATCCCCAACGGCATTGATCAGCACAAAGCCGAGCCAGCATGCCAGTAACAAAAATCCGCAAACTTCATGCACCGCAACCAGAC  >  NC_000913/1747870‑1748056

AAAGGATGTTCTTCCCCCTGCATAATGCCAAACAAATAAAATCGCGTTTGTTTTGCCGCTCGTTCCAGCCACCCCTGACGACGAATGCGATAGC                                                                                               <  SRR4302533.2165580/94‑1 (MQ=60)

 AAGGATGTTCTTCCCCCTGCATAATGCCAAACAAATAAAATCGCGTTTGTTTTGCCGCTCGTTCCAGCCACCCCTGACGACGAATGCGATAGCG                                                                                              <  SRR4302533.973107/94‑1 (MQ=60)

  AGGATGTTCTTCCCCCTGCATAATGCCAAACAAATAAAATCGCGTTTGTTTTGCCGCTCGTTCCAGCCACCCCTGACGACGAATGCGATAGCGG                                                                                             >  SRR4302533.5282085/1‑94 (MQ=60)

   GGATGTTCTTCCCCCTGCATAATGCCAAACAAATAAAATCGCGTTTGTTTTGCCGCTCGTTCCAGCCACCCCTGACGACGAATGCGATAGCGGT                                                                                            <  SRR4302533.5702753/94‑1 (MQ=60)

    GATGTTCTTCCCCCTGCATAATGCCAAACAAATAAAATCGCGTTTGTTTTGCCGCTCGTTCCAGCCACCCCTGACGACGAATGCGATAGCGGTG                                                                                           <  SRR4302533.919185/94‑1 (MQ=60)

    GATGTTCTTCCCCCTGCATAATGCCAAACAAATAAAATCGCGTTTGTTTTGCCGCTCGTTCCAGCCACCCCTGACGACGAATGCGATAGCGGTG                                                                                           <  SRR4302533.1946456/94‑1 (MQ=60)

     ATGTTCTTCCCCCTGCATAATGCCAAACAAATAAAATCGCGTTTGTTTTGCCGCTCGTTCCAGCCACCCCTGACGACGAATGCGATAGCGGTGA                                                                                          >  SRR4302533.4438514/1‑94 (MQ=60)

     ATGTTCTTCCCCCTGCATAATGCCAAACAAATAAAATCGCGTTTGTTTTGCCGCTCGTTCCAGCCACCCCTGACGACGAATGCGATAGCGGAGA                                                                                          >  SRR4302533.2380463/1‑94 (MQ=60)

      TGTTCTTCCCCCTGCATAATGCCAAACAAATAAAATCGCGTTTGTTTTGCCGCTCGTTCCAGCCACCCCTGACGACGAATGCGATAGCGGTGAC                                                                                         >  SRR4302533.5308203/1‑94 (MQ=60)

      TGTTCTTCCCCCTGCATAATGCCAAACAAATAAAATCGCGTTTGTTTTGCCGCTCGTTCCAGCCACCCCTGACGACGAATGCGATAGCGGTGAC                                                                                         <  SRR4302533.1177095/94‑1 (MQ=60)

       GTTCTTCCCCCTGCATAATGCCAAACAAATAAAATCGCGTTTGTTTTGCCGCTCGTTCCAGCCACCCCTGACGACGAATGCGATAGCGGTGACA                                                                                        >  SRR4302533.1998280/1‑94 (MQ=60)

        TTCTTCCCCCTGCATAATGCCAAACAAATAAAATCGCGTTTGTTTTGCCGCTCGTTCCAGCCACCCCTGACGACGAATGCGATAGCGGTGACCA                                                                                       <  SRR4302533.2866052/94‑1 (MQ=60)

         TCTTCCCCCTGCATAATGCCAAACAAATAAAATCGCGTTTGTTTTGCCGCTCGTTCCAGCCACCCCTGACGACGAATGCGATAGCGGTGACCAT                                                                                      <  SRR4302533.5242789/94‑1 (MQ=60)

         TCTTCCCCCTGCATAATGCCAAACAAATAAAATCGCGTTTGTTTTGCCGCTCGTTCCAGCCACCCCTGACGACGAATGCGATAGCGGTGACCAT                                                                                      <  SRR4302533.64272/94‑1 (MQ=60)

             CCCCCTGCATAATGCCAAACAAATAAAATCGCGTTTGTTTTGCCGCTCGTTCCAGCCACCCCTGACGACGAATGCGATAGCGGTGACCATTATC                                                                                  <  SRR4302533.5487894/94‑1 (MQ=60)

             CCCCCTGCATAATGCCAAACAAATAAAATCGCGTTTGTTTTGCCGCTCGTTCCAGCCACCCCTGACGACGAATGCGATAGCGGTGACCATTATC                                                                                  >  SRR4302533.2110563/1‑94 (MQ=60)

             CCCCCTGCATAATGCCAAACAAATAAAATCGCGTTTGTTTTGCCGCTCGTTCCAGCCACCCCTGACGACGAATGCGATAGCGGTGACCATTATC                                                                                  >  SRR4302533.1885139/1‑94 (MQ=60)

             CCCCCTGCATAATGCCAAACAAATAAAATCGCGTTTGTTTTGCCGCTCGTTCCAGCCACCCCTGACGACGAATGCGATAGCGGTGACCATTATC                                                                                  >  SRR4302533.5162662/1‑94 (MQ=60)

             CCCCCTGCATAATGCCAAACAAATAAAATCGCGTTTGTTTTGCCGCTCGTTCCAGCCACCCCTGACGACGAATGCGATAGAGGTGACCATTATC                                                                                  >  SRR4302533.2706140/1‑94 (MQ=60)

              CCCCTGCATAATGCCAAACAAATAAAATCGCGTTTGTTTTGCCGCTCGTTCCAGCCACCCCTGACGACGAATGCGATAGCGGTGACCATTATCC                                                                                 <  SRR4302533.4256562/94‑1 (MQ=60)

               CCCTGCATAATGCCAAACAAATAAAATCGCGTTTGTTTTGCCGCTCGTTCCAGCCACCCCTGACGACGAATGCGATAGCGGTGACCATTATCCC                                                                                >  SRR4302533.275153/1‑94 (MQ=60)

               CCCTGCATAATGCCAAACAAATAAAATCGCGTTTGTTTTGCCGCTCGTTCCAGCCACCCCTGACGACGAATGCGATAGCGGTGACCATTATCCC                                                                                <  SRR4302533.2025106/94‑1 (MQ=60)

               CCCTGCATAATGCCAAACAAATAAAATCGCGTTTGTTTTGCCGCTCGTTCCAGCCACCCCTGACGACGAATGCGATAGCGGTGACCATTATCCC                                                                                >  SRR4302533.701293/1‑94 (MQ=60)

                CCTGCATAATGCCAAACAAATAAAATCGCGTTTGTTTTGCCGCTCGTTCCAGCCACCCCTGACGACGAATGCGATAGCGGTGACCATTATCCCC                                                                               >  SRR4302533.1847471/1‑94 (MQ=60)

                CCTGCATAATGCCAAACAAATAAAATCGCGTTTGTTTTGCCGCTCGTTCCAGCCACCCCTGACGACGAATGCGATAGCGGTGACCATTATCCCC                                                                               >  SRR4302533.4583647/1‑94 (MQ=60)

                 CTGCATAATGCCAAACAAATAAAATCGCGTTTGTTTTGCCGCTCGTTCCAGCCACCCCTGACGACGAATGCGATAGCGGTGACCATTATCCCCA                                                                              >  SRR4302533.2471955/1‑94 (MQ=60)

                  TGCATAATGCCAAACAAATAAAATCGCGTTTGTTTTGCCGCTCGTTCCAGCCACCCCTGACGACGAATGCGATAGCGGTGACCATTATCCCCAA                                                                             >  SRR4302533.354156/1‑94 (MQ=60)

                    CATAATGCCAAACAAATAAAATCGCGTTTGTTTTGCCGCTCGTTCCAGCCACCCCTGACGACGAATGCGATAGCGGTGACCATTATCCCCAACG                                                                           <  SRR4302533.494729/94‑1 (MQ=60)

                    CATAATGCCAAACAAATAAAATCGCGTTTGTTTTGCCGCTCGTTCCAGCCACCCCTGACGACGAATGCGATAGCGGTGACCATTATCCCCAACG                                                                           <  SRR4302533.3662833/94‑1 (MQ=60)

                    CATAATGCCAAACAAATAAAATCGCGTTTGTTTTGCCGCTCGTTCCAGCCACCCCTGACGACGAATGCGATAGCGGTGACCATTATCCCCAACG                                                                           >  SRR4302533.204305/1‑94 (MQ=60)

                    CATAATGCCAAACAAATAAAATCGCGTTTGTTTTGCCGCTCGTTCCAGCCACCCCTGACGACGAATGCGATAGCGGTGACCATTATCCCCAACG                                                                           <  SRR4302533.6232153/94‑1 (MQ=60)

                     ATAATGCCAAACAAATAAAATCGCGTTTGTTTTGCCGCTCGTTCCAGCCACCCCTGACGACGAATGCGATAGCGGTGACCATTATCCCCAACGG                                                                          <  SRR4302533.2451862/94‑1 (MQ=60)

                     ATAATGCCAAACAAATAAAATCGCGTTTGTTTTGCCGCTCGTTCCAGCCACCCCTGACGACGAATGCGATAGCGGTGACCATTATCCCCAACGG                                                                          >  SRR4302533.5946003/1‑94 (MQ=60)

                        ATGCCAAACAAATAAAATCGCGTTTGTTTTGCCGCTCGTTCCAGCCACCCCTGACGACGAATGCGATAGCGGTGACCATTATCCCCAACGGCAT                                                                       >  SRR4302533.1777489/1‑94 (MQ=60)

                         AGCCAAACAAATAAAATCGCGTTTGTTTTGCCGCTCGTTCCAGCCACCCCTGACGACGAATGCGATAGCGGTGACCATTATCCCCAACGGCATT                                                                      <  SRR4302533.4504786/94‑1 (MQ=60)

                          GCCAAACAAATAAAATCGCGTTTGTTTTGCCGCTCGTTCCAGCCACCCCTGACGACGAATGCGATAGCGGTGACCATTATCCCCAACGGCATTG                                                                     <  SRR4302533.4297235/94‑1 (MQ=60)

                           CCAAACAAATAAAATCGCGTTTGTTTTGCCGCTCGTTCCAGCCACCCCTGACGACGAATGCGATAGCGGTGACCATTATCCCCAACGGCATTGA                                                                    >  SRR4302533.5184601/1‑94 (MQ=60)

                           CCAAACAAATAAAATCGCGTTTGTTTTGCCGCTCGTTCCAGCCACCCCTGACGACGAATGCGATAGCGGTGACCATTATCCCCAACGGCATTGA                                                                    <  SRR4302533.3101982/94‑1 (MQ=60)

                            CAAACAAATAAAATCGCGTTTGTTTTGCCGCTCGTTCCAGCCACCCCTGACGACGAATGCGATAGCGGTGACCATTATCCCCAACGGCATTGAT                                                                   >  SRR4302533.4097261/1‑94 (MQ=60)

                            CAAACAAATAAAATCGCGTTTGTTTTGCCGCTCGTTCCAGCCACCCCTGACGACGAATGCGATAGCGGTGACCATTATCCCCAACGGCATTGAT                                                                   >  SRR4302533.2791753/1‑94 (MQ=60)

                             AAACAAATAAAATCGCGTTTGTTTTGCCGCTCGTTCCAGCCACCCCTGACGACGAATGCGATAGCGGTGACCATTATCCCCAACGGCATTGATC                                                                  <  SRR4302533.573190/94‑1 (MQ=60)

                              AACAAATAAAATCGCGTTTGTTTTGCCGCTCGTTCCAGCCACCCCTGACGACGAATGCGATAGCGGTGACCATTATCCCCAACGGCATTGATCA                                                                 >  SRR4302533.5247138/1‑94 (MQ=60)

                              AACAAATAAAATCGCGTTTGTTTTGCCGCTCGTTCCAGCCACCCCTGACGACGAATGCGATAGCGGTGACCATTATCCCCAACGGCATTGATCA                                                                 >  SRR4302533.5646447/1‑94 (MQ=60)

                              AACAAATAAAATCGCGTTTGTTTTGCCGCTCGTTCCAGCCACCCCTGACGACGAATGCGATAGCGGTGACCATTATCCCCAACGGCATTGATCA                                                                 <  SRR4302533.1661739/94‑1 (MQ=60)

                              AACAAATAAAATCGCGTTTGTTTTGCCGCTCGTCCCAGCCACCCCTGACGACGAATGCGATAGCGGTGACCATTATCCCCAACGGCATTGATCA                                                                 <  SRR4302533.4639796/94‑1 (MQ=60)

                               ACAAATAAAATCGCGTTTGTTTTGCCGCTCGTTCCAGCCACCCCTGACGACGAATGCGATAGCGGTGACCATTATCCCCAACGGCATTGATCAG                                                                <  SRR4302533.1031637/94‑1 (MQ=60)

                               ACAAATAAAATCGCGTTTGTTTTGCCGCTCGTTCCAGCCACCCCTGACGACGAATGCGATAGCGGTGACCATTATCCCCAACGGCATTGATCAG                                                                >  SRR4302533.2129359/1‑94 (MQ=60)

                                CAAATAAAATCGCGTTTGTTTTGCCGCTCGTTCCAGCCACCCCTGACGACGAATGCGATAGCGGTGACCATTATCCCCAACGGCATTGATCAGC                                                               <  SRR4302533.2816771/94‑1 (MQ=60)

                                CAAATAAAATCGCGTTTGTTTTGCCGCTCGTTCCAGCCACCCCTGACGACGAATGCGATAGCGGTGACCATTATCCCCAACGGCATTGATCAGC                                                               >  SRR4302533.1628263/1‑94 (MQ=60)

                                CAAATAAAATCGCGTTTGTTTTGCCGCTCGGTCCAGCCACCCCTGACGACGAATGCGATAGCGGTGACCATTATCCCCAACGGCATTGATCAGC                                                               >  SRR4302533.5675048/1‑94 (MQ=60)

                                  AATAAAATCGCGTTTGTTTTGCCGCTCGTTCCAGCCACCCCTGACGACGAATGCGATAGCGGTGACCATTATCCCCAACGGCATTGATCAGCAC                                                             <  SRR4302533.1532914/94‑1 (MQ=60)

                                   ATAAAATCGCGTTTGTTTTGCCGCTCGTTCCAGCCACCCCTGACGACGAATGCGATAGCGGTGACCATTATCCCCAACGGCATTGATCAGCACA                                                            >  SRR4302533.300101/1‑94 (MQ=60)

                                      AAATCGCGTTTGTTTTGCCGCTCGTTCCAGCCACCCCTGACGACGAATGCGATAGCGGTGACCATTATCCCCAACGGCATTGATCAGCACAAAG                                                         <  SRR4302533.1325895/94‑1 (MQ=60)

                                        ATCGCGTTTGTTTTGCCGCTCGTTCCAGCCACCCCTGACGACGAATGCGATAGCGGTGACCATTATCCCCAACGGCATTGATCAGCACAAcgcg                                                       >  SRR4302533.4133417/1‑90 (MQ=60)

                                        ATCGCGTTTGTTTTGCCGCTCGTTCCAGCCACCCCTGACGACGAATGCGATAGCGGTGACCATTATCCCCAACGGCATTGATCAGCACAAAGCC                                                       >  SRR4302533.4959154/1‑94 (MQ=60)

                                         TCGCGTTTGTTTTGCCGCTCGTTCCAGCCACCCCTGACGACGAATGCGATAGCGGTGACCATTATCCCCAACGGCATTGATCAGCACAAAGCCG                                                      <  SRR4302533.5096665/94‑1 (MQ=60)

                                         TCGCGTTTGTTTTGCCGCTCGTTCCAGCCACCCCTGACGACGAATGCGATAGCGGTGACCATTATCCCCAACGGCATTGATCAGCACAAAGCCG                                                      <  SRR4302533.1090003/94‑1 (MQ=60)

                                           GCGTTTGTTTTGCCGCTCGTTCCAGCCACCCCTGACGACGAATGCGATAGCGGTGACCATTATCCCCAACGGCATTGATCAGCACAAAGCCGAG                                                    <  SRR4302533.5049502/94‑1 (MQ=60)

                                           GCGTTTGTTTTGCCGCTCGTTCCAGCCACCCCTGACGACGAATGCGATAGCGGTGACCATTATCCCCAACGGCATTGATCAGCACAAAGCCGAG                                                    <  SRR4302533.4061022/94‑1 (MQ=60)

                                           GCGTTTGTTTTGCCGCTCGTTCCAGCCACCCCTGACGACGAATGCGATAGCGGTGACCATTATCCCCAACGGCATTGATCAGCACAAAGCCGAG                                                    >  SRR4302533.1341499/1‑94 (MQ=60)

                                           GCGTTTGTTTTGCCGCTCGTTCCAGCCACCCCTGACGACGAATGCGATAGCGGTGACCATTATCCCCAACGGCATTGATCAGCACAAAGCCGAG                                                    >  SRR4302533.3364558/1‑94 (MQ=60)

                                            CGTTTGTTTTGCCGCTCGTTCCAGCCACCCCTGACGACGAATGCGATAGCGGTGACCATTATCCCCAACGGCATTGATCAGCACAAAGCCGAGC                                                   <  SRR4302533.5112938/94‑1 (MQ=60)

                                             GTTTGTTTTGCCGCTCGTTCCAGCCACCCCTGACGACGAATGCGATAGCGGTGACCATTATCCCCAACGGCATTGATCAGCACAAAGCCGAGCC                                                  >  SRR4302533.6068616/1‑94 (MQ=60)

                                             GTTTGTTTTGCCGCTCGTTCCAGCCACCCCTGACGACGAATGCGATAGCGGTGACCATTATCCCCAACGGCATTGATCAGCACAAAGCCGAGCC                                                  >  SRR4302533.1225/1‑94 (MQ=60)

                                             GTTTGTTTTGCCGCTCGTTCCAGCCACCCCTGACGACGAATGCGATAGCGGTGACCATTATCCCCAACGGCATTGATCAGCACAAAGCCGAGCC                                                  >  SRR4302533.2135466/1‑94 (MQ=60)

                                                TGTTTTGCCGCTCGTTCCAGCCACCCCTGACGACGAATGCGATAGCGGTGACCATTATCCCCAACGGCATTGATCAGCACAAAGCCGAGCCAGC                                               >  SRR4302533.3179908/1‑94 (MQ=60)

                                                TGTTTTGCCGCTCGTTCCAGCCACCCCTGACGACGAATGCGATAGCGGTGACCATTATCCCCAACGGCATTGATCAGCACAAAGCCGAGCCAGC                                               >  SRR4302533.4982729/1‑94 (MQ=60)

                                                 GTTTTGCCGCTCGTTCCAGCCACCCCTGACGACGAATGCGATAGCGGTGACCATTATCCCCAACGGCATTGATCAGCACAAAGCCGAGCCAGCA                                              >  SRR4302533.1521032/1‑94 (MQ=60)

                                                 GTTTTGCCGCTCGTTCCAGCCACCCCTGACGACGAATGCGATAGCGGTGACCATTATCCCCAACGGCATTGATCAGCACAAAGCCGAGCCAGCA                                              <  SRR4302533.5625097/94‑1 (MQ=60)

                                                 GTTTTGCCGCTCGTTCCAGCCACCCCTGACGACGAATGCGATAGCGGTGACCATTATCCCCAACGGCATTGATCAGCACAAAGCCGAGCCAGCA                                              >  SRR4302533.3603261/1‑94 (MQ=60)

                                                 GTTTTGCCGCTCGTTCCAGCCACCCCTGACGACGAATGCGATAGCGGTGACCATTATCCCCAACGGCATTGATCAGCACAAAGCCGAGCCAGCA                                              >  SRR4302533.4535433/1‑94 (MQ=60)

                                                  TTTTGCCGCTCGTTCCAGCCACCCCTGACGACGAATGCGATAGCGGTGACCATTATCCCCAACGGCATTGATCAGCACAAAGCCGAGCCCGCAT                                             >  SRR4302533.5051967/1‑94 (MQ=60)

                                                  TTTTGCCGCTCGTTCCAGCCACCCCTGACGACGAATGCGATAGCGGTGACCATTATCCCCAACGGCATTGATCAGCACAAAGCCGAGCCAGCAT                                             <  SRR4302533.1246223/94‑1 (MQ=60)

                                                   TTTGCCGCTCGTTCCAGCCACCCCTGACGACGAATGCGATAGCGGTGACCATTATCCCCAACGGCATTGATCAGCACAAAGCCGAGCCAGCATG                                            <  SRR4302533.4667033/94‑1 (MQ=60)

                                                     TGCCGCTCGTTCCAGCCACCCCTGACGACGAATGCGATAGCGGTGACCATTATCCCCAACGGCATTGATCAGCACAAAGCCGAGCCAGCATGCC                                          >  SRR4302533.2424690/1‑94 (MQ=60)

                                                     TGCCGCTCGTTCCAGCCACCCCTGACGACGAATGCGATAGCGGTGACCATTATCCCCAACGGCATTGATCAGCACAAAGCCGAGCCAGCATGCC                                          >  SRR4302533.4708827/1‑94 (MQ=60)

                                                       CCGCTCGTTCCAGCCACCCCTGACGACGAATGCGATAGCGGTGACCATTATCCCCAACGGCATTGATCAGCACAAAGCCGAGCCAGCATGCCAG                                        >  SRR4302533.689632/1‑94 (MQ=60)

                                                       CCGCTCGTTCCAGCCACCCCTGACGACGAATGCGATAGCGGTGACCATTATCCCCAACGGCATTGATCAGCACAAAGCCGAGCCAGCATGCCAG                                        <  SRR4302533.4804102/94‑1 (MQ=60)

                                                       CCGCTCGTTCCAGCCACCCCTGACGACGAATGCGATAGCGGTGACCATTATCCCCAACGGCATTGATCAGCACAAAGCCGAGCCAGCATGCCAG                                        >  SRR4302533.2791657/1‑94 (MQ=60)

                                                        CGCTCGTTCCAGCCACCCCTGACGACGAATGCGATAGCGGTGACCATTATCCCCAACGGCATTGATCAGCACAAAGCCGAGCCAGCATGCCAGT                                       <  SRR4302533.5387014/94‑1 (MQ=60)

                                                        CGCTCGTTCCAGCCACCCCTGACGACGAATGCGATAGCGGTGACCATTATCCCCAACGGCATTGATCAGCACAAAGCCGAGCCAGCATGCCAGT                                       <  SRR4302533.5068671/94‑1 (MQ=60)

                                                        CGCTCGTTCCAGCCACCCCTGACGACGAATGCGATAGCGGTGACCATTATCCCCAACGGCATTGATCAGCACAAAGCCGAGCCAGCATGCCAGT                                       >  SRR4302533.2253019/1‑94 (MQ=60)

                                                        CGCTCGTTCCAGCCACCCCTGACGACGAATGCGATACCGGTGACCATTATCCCCAACGGCATTGATCAGCACAAAGCCGAGCCAGCATGCCAGT                                       >  SRR4302533.2723173/1‑94 (MQ=60)

                                                           TCGTTCCAGCCACCCCTGACGACGAATGCGATAGCGGTGACCATTATCCCCAACGGCATTGATCAGCACAAAGCCGAGCCAGCATGCCAGTAAC                                    >  SRR4302533.3605922/1‑94 (MQ=60)

                                                           TCGTTCCAGCCACCCCTGACGACGAATGCGATAGCGGTGACCATTATCCCCAACGGCATTGATCAGCACAAAGCCGAGCCAGCATGCCAGTAAC                                    >  SRR4302533.1560753/1‑94 (MQ=60)

                                                            CGTTCCAGCCACCCCTGACGTCGAATGCGATAGCGGCGACCATTATCCCCAACGGCATTGATCAGCACAAAGCCGAGCCAGCATGCCAGTACCA                                   >  SRR4302533.5049116/1‑94 (MQ=60)

                                                             GTTCCAGCCACCCCTGACGACGAATGCGATAGCGGTGACCATTATCCCCAACGGCATTGATCAGCACAAAGCCGAGCCAGCATGCCAGTAACAA                                  >  SRR4302533.3903251/1‑94 (MQ=60)

                                                              TTCCAGCCACCCCTGACGACGAATGCGATAGCGGTGACCATTATCCCCAACGGCATTGATCAGCACAAAGCCGAGCCAGCATGCCAGTAACAAA                                 <  SRR4302533.3835059/94‑1 (MQ=60)

                                                              TTCCAGCCACCCCTGACGACGAATGCGATAGCGGTGACCATTATCCCCAACGGCATTGATCAGCACAAAGCCGAGCCAGCATGCCAGTAACAAA                                 <  SRR4302533.895235/94‑1 (MQ=60)

                                                               TCCAGCCACCCCTGACGACGAATGCGATAGCGGTGACCATTATCCCCAACGGCATTGATCAGCACAAAGCCGAGCCAGCATGCCAGTAACAAAA                                >  SRR4302533.2642376/1‑94 (MQ=60)

                                                                CCAGCCACCCCTGACGACGAATGCGATAGCGGTGACCATTATCCCCAACGGCATTGATCAGCACAAAGCCGAGCCAGCATGCCAGTAACAAAAA                               >  SRR4302533.5120023/1‑94 (MQ=60)

                                                                 CAGCCACCCCTGACGACGAATGCGATAGCGGTGACCATTATCCCCAACGGCATTGATCAGCACAAAGCCGAGCCAGCATGCCAGTAACAAAAAT                              >  SRR4302533.2510290/1‑94 (MQ=60)

                                                                 CAGCCACCCCTGACGACGAATGCGATAGCGGTGACCATTATCCCCAACGGCATTGATCAGCACAAAGCCGAGCCAGCATGCCAGTAACAAAAAT                              <  SRR4302533.1477042/94‑1 (MQ=60)

                                                                  AGCCACCCCTGACGACGAATGCGATAGCGGTGACCATTATCCCCAACGGCATTGATCAGCACAAAGCCGAGCCAGCATGCCAGTAACAAAAATC                             >  SRR4302533.992350/1‑94 (MQ=60)

                                                                  AGCCACCCCTGACGACGAATGCGATAGCGGTGACCATTATCCCCAACGGCATTGATCAGCACAAAGCCGAGCCAGCATGCCAGTAACAAAAATC                             <  SRR4302533.3320311/94‑1 (MQ=60)

                                                                  AGCCACCCCTGACGACGAATGCGATAGCGGTGACCATTATCCCCAACGGCATTGATCAGCACAAAGCCGAGCCAGCATGCCAGTAACAAAAATC                             >  SRR4302533.1375058/1‑94 (MQ=60)

                                                                    cctcccacgacgagtAATGCGATAGGGGTGCCCATTATCCCCAGCGGCATTGATCAGCACAAAGCCGAGCCAGCATGCCAGTAACAAAAATCCG                           <  SRR4302533.1412114/79‑1 (MQ=60)

                                                                    CCACCCCTGACGACGAATGCGATAGCGGTGACCATTATCCCCAACGGCATTGATCAGCACAAAGCCGAGCCAGCATGCCAGTAACAAAAATCCG                           >  SRR4302533.5970307/1‑94 (MQ=60)

                                                                     CACCCCTGACGACGAATGCGATAGCGGTGACCATTATCCCCAACGGCATTGATCAGCACAAAGCCGAGCCAGCATGCCAGTAACAAAAATCCGC                          >  SRR4302533.4530087/1‑94 (MQ=60)

                                                                      ACCCCTGACGACGAATGCGATAGCGGTGACCATTATCCCCAACGGCATTGATCAGCACAAAGCCGAGCCAGCATGCCAGTAACAAAAATCCGCA                         >  SRR4302533.370345/1‑94 (MQ=60)

                                                                        CCCTGACGACGAATGCGATAGCGGTGACCATTATCCCCAACGGCATTGATCAGCACAAAGCCGAGCCAGCATGCCAGTAACAAAAATCCGCAAA                       >  SRR4302533.2875739/1‑94 (MQ=60)

                                                                        CCCTGACGACGAATGCGATAGCGGTGACCATTATCCCCAACGGCATTGATCAGCACAAAGCCGAGCCAGCATGCCAGTAACAAAAATCCGCAAA                       >  SRR4302533.53851/1‑94 (MQ=60)

                                                                        CCCTGACGACGAATGCGATAGCGGTGACCATTATCCCCAACGGCATTGATCAGCACAAAGCCGAGCCAGCATGCCAGTAACAAAAATCCGCAAA                       >  SRR4302533.5209335/1‑94 (MQ=60)

                                                                        CCCTGACGACGAATGCGATAGCGGTGACCATTATCCCCAACGGCATTGATCAGCACAAAGCCGAGCCAGCATGCCAGTAACAAAAATCCGCAAA                       <  SRR4302533.2351996/94‑1 (MQ=60)

                                                                         CCTGACGACGAATGCGATAGCGGTGACCATTATCCCCAACGGCATTGATCAGCACAAAGCCGAGCCAGCATGCCAGTAACAAAAATCCGCAAAC                      >  SRR4302533.5474355/1‑94 (MQ=60)

                                                                          CTGACGACGAATGCGATAGCGGTGACCATTATCCCCAACGGCATTGATCAGCACAAAGCCGAGCCAGCATGCCAGTAACAAAAATCCGCAAACT                     >  SRR4302533.148269/1‑94 (MQ=60)

                                                                          CTGACGACGAATGCGATAGCGGGGACCATTATCCCCAACGGCATTGATCAGCACAAAGCCGAGCCAGCATGCCAGTAACAAAAATCCGCAAACT                     >  SRR4302533.5670657/1‑94 (MQ=60)

                                                                             ACGACGAATGCGATAGCGGTGACCATTATCCCCAACGGCATTGATCAGCACAAAGCCGAGCCAGCATGCCAGTAACAAAAATCCGCAAACTTCA                  <  SRR4302533.2328399/94‑1 (MQ=60)

                                                                             ACGACGAATGCGATAGCGGTGACCATTATCCCCAACGGCATTGATCAGCACAAAGCCGAGCCAGCATGCCAGTAACAAAAATCCGCAAACTTCA                  <  SRR4302533.2552880/94‑1 (MQ=60)

                                                                              CGACGAATGCGATAGCGGTGACCATTATCCCCAACGGCATTGATCAGCACAAAGCCGAGCCAGCATGCCAGTAACAAAAATCCGCAAACTTCAT                 <  SRR4302533.6264961/94‑1 (MQ=60)

                                                                              CGACGAATGCGATAGCGGTGACCATTATCCCCAACGGCATTGATCAGCACAAAGCCGAGCCAGCATGCCAGTAACAAAAATCCGCAAACTTCAT                 >  SRR4302533.430082/1‑94 (MQ=60)

                                                                              CGACGAATGCGATAGCGGTGACCATTATCCCCAACGGCATTGATCAGCACAAAGCCGAGCCAGCATGCCAGTAACAAAAATCCGCAAACTTCAT                 >  SRR4302533.5296073/1‑94 (MQ=60)

                                                                               GACGAATGCGATAGCGGTGACCATTATCCCCAACGGCATTGATCAGCACAAAGCCGAGCCAGCATGCCAGTAACAAAAATCCGCAAACTTCATG                <  SRR4302533.4271688/94‑1 (MQ=60)

                                                                                ACGAATGCGATAGCGGTGACCATTATCCCCAACGGCATTGATCAGCACAAAGCCGAGCCAGCATGCCAGTAACAAAAATCCGCAAACTTCATGC               <  SRR4302533.791297/94‑1 (MQ=60)

                                                                                  GAATGCGATAGCGGTGACCATTATCCCCAACGGCATTGATCAGCACAAAGCCGAGCCAGCATGCCAGTAACAAAAATCCGCAAACTTCATGCAC             >  SRR4302533.6317293/1‑94 (MQ=60)

                                                                                  GAATGCGATAGCGGTGACCATTATCCCCAACGGCATTGATCAGCACAAAGCCGAGCCAGCATGCCAGTAACAAAAATCCGCAAACTTCATGCAC             >  SRR4302533.918949/1‑94 (MQ=60)

                                                                                   AATGCGATAGCGGTGACCATTATCCCCAACGGCATTGATCAGCACAAAGCCGAGCCAGCATGCCAGTAACAAAAATCCGCAAACTTCATGCACC            >  SRR4302533.2603106/1‑94 (MQ=60)

                                                                                   AATGCGATAGCGGTGACCATTATCCCCAACGGCATTGATCAGCACAAAGCCGAGCCAGCATGCCAGTAACAAAAATCCGCAAACTTCATGCACC            >  SRR4302533.4236585/1‑94 (MQ=60)

                                                                                    ATGCGATAGCGGTGACCATTATCCCCAACGGCATTGATCAGCACAAAGCCGAGCCAGCATGCCAGTAACAAAAATCCGCAAACTTCATGCACCG           >  SRR4302533.1341287/1‑94 (MQ=60)

                                                                                     TGCGATAGCGGTGACCATTATCCCCAACGGCATTGATCAGCACAAAGCCGAGCCAGCATGCCAGTAACAAAAATCCGCAAACTTCATGCACCGC          <  SRR4302533.3138994/94‑1 (MQ=60)

                                                                                        GATAGCGGTGACCATTATCCCCAACGGCATTGATCAGCACAAAGCCGAGCCAGCATGCCAGTAACAAAAATCCGCAAACTTCATGCACCGCAAC       >  SRR4302533.4628069/1‑94 (MQ=60)

                                                                                        GATAGCGGTGACCATTATCCCCAACGGCATTGATCAGCACAAAGCCGAGCCAGCATGCCAGTAACAAAAATCCGCAAACTTCATGCACCGCAAC       <  SRR4302533.3030591/94‑1 (MQ=60)

                                                                                           AGCGGTGACCATTATCCCCAACGGCATTGATCAGCACAAAGCCGAGCCAGCATGCCAGTAACAAAAATCCGCAAACTTCATGCACCGCAACCAG    <  SRR4302533.1553587/94‑1 (MQ=60)

                                                                                           AGCGGTGACCATTATCCCCAACGGCATTGATCAGCACAAAGCCGAGCCAGCATGCCAGTAACAAAAATCCGCAAACTTCATGCACCGCAACCAG    >  SRR4302533.645552/1‑94 (MQ=60)

                                                                                           AGCGGTGACCATTATCCCCAACGGCATTGATCAGCACAAAGCCGAGCCAGCATGCCAGTAACAAAAATCCGCAAACTTCATGCACCGCAACCAG    >  SRR4302533.789190/1‑94 (MQ=60)

                                                                                             CGGTGACCATTATCCCCAACGGCATTGATCAGCACAAAGCCGAGCCAGCATGCCAGTAACAAAAATCCGCAAACTTCATGCACCGCAACCAGAC  >  SRR4302533.2268537/1‑94 (MQ=60)

                                                                                             CGGTGACCATTATCCCCAACGGCATTGATCAGCACAAAGCCGAGCCAGCATGCCAGTAACAAAAATCCGCAAACTTCATGCACCGCAACCAGAC  <  SRR4302533.803230/94‑1 (MQ=60)

AAAGGATGTTCTTCCCCCTGCATAATGCCAAACAAATAAAATCGCGTTTGTTTTGCCGCTCGTTCCAGCCACCCCTGACGACGAATGCGATAGTGGTGACCATTATCCCCAACGGCATTGATCAGCACAAAGCCGAGCCAGCATGCCAGTAACAAAAATCCGCAAACTTCATGCACCGCAACCAGAC  >  NC_000913/1747870‑1748056

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 7 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`