Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1624	250.6	12620048	98.0%	12367647	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,962,070	A→T	P335P (CCT→CCA)	flhA ←	FlhA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,962,070	0	A	T	99.5%	680.4 / NA	184	P335P (CCT→CCA)	flhA	FlhA
Reads supporting (aligned to +/- strand): ref base A (0/0); major base T (95/88); minor base G (0/1); total (95/89)
Fisher's exact test for biased strand distribution p-value = 4.84e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 193 total aligned reads displayed.

ATTCCCAGAGAATCTTCCAGTTGTACATCGTTCCACGTCGCTTCGACAACGGTATTATTCTCTGCCATTTTTACCGGTTTGGGTTCGGCAGGCGCTTTTTGTTCGCGTCCGCGTATCCACCAGGCCAGCCCGAGCAATCCGGCAGTGAACAGCAAAAATACCAGGTTCGGCATTCCAGG  >  NC_000913/1961981‑1962159

aTTCCCAGAGAATCTTCCAGTTGTACATCGTTCCACGTCGCTTCGACAACGGTATTATTCTCTGCCATTTTTACCGGTTTGGGTTCGGCTGgc                                                                                        <  2:2849367/93‑1 (MQ=255)

 ttCCCAGAGAATCTTCCAGTTGTACATCGTTCCACGTCGCTTCGACAACGGTATTATTCTCTGCCATTTTTACCGGTTTGGGTTCGGCTgggg                                                                                       >  2:3300313/1‑91 (MQ=255)

 ttCCCAGAGAATCTTCCAGTTGTACATCGTTCCACGTCGCTTCGACAACGGTATTATTCTCTGCCATTTTTACCGGTTTGGGTTCGGCTGgcg                                                                                       >  1:1180792/1‑93 (MQ=255)

 ttCCCAGAGAATCTTCCAGTTGTACATCGTTCCACGTCGCTTCGACAACGGTATTATTCTCTGCCATTTTTACCGGTTTGGGTTCGGCTGgcg                                                                                       <  2:2020983/93‑1 (MQ=255)

 ttCCCAGAGAATCTTCCAGTTGTACATCGTTCCACGTCGCTTCGACAACGGTATTATTCTCTGCCATTTTTACCGGTTTGGGTTCGGCTGgcg                                                                                       >  1:2427791/1‑93 (MQ=255)

  tCCCAGAGAATCTTCCAGTTGTACATCGTTCCACGTCGCTTCGACAACGGTATTATTCTCTGCCATTTTTACCGGTTTGGGTTCGGCTGgcgc                                                                                      >  1:1011219/1‑93 (MQ=255)

   cccAGAGAATCTTCCAGTTGTACATCGTTCCACGTCGCTTCGACAACGGTATTATTCTCTGCCATTTTTACCGGTTTGGGTTCGGCTGGCGCt                                                                                     >  1:803357/1‑93 (MQ=255)

    ccAGAGAATCTTCCAGTTGTACATCGTTCCACGTCGCTTCGACAACGGTATTATTCTCTGCCATTTTTACCGGTTTGGGTTCGGCTGGCGCtt                                                                                    >  2:1541723/1‑93 (MQ=255)

    ccAGAGAATCTTCCAGTTGTACATCGTTCCACGTCGCTTCGACAACGGTATTATTCTCTGCCATTTTTACCGGTTTGGGGTCGGCTGGCGCtt                                                                                    >  2:1323578/1‑93 (MQ=255)

     cAGAGAATCTTCCAGTTGTACATCGTTCCACGTCGCTTCGACAACGGTATTATTCTCTGCCATTTTTACCGGTTTGGGTTCGGCTGGCGCttt                                                                                   >  2:3943736/1‑93 (MQ=255)

     cAGAGAATCTTCCAGTTGTACATCGTTCCACGTCGCTTCGACAACGGTATTATTCTCTGCCATTTTTACCGGTTTGGGTTCGGCTGGCGCttt                                                                                   >  1:6070940/1‑93 (MQ=255)

        agaATCTTGCATTTGTACATCGTTCCACGTCGCTTCGACAACGGTATTATTCTCTGCCATTTTTACCGGTTTGGGTTCGGCTTTCGCTTTTTg                                                                                <  2:1178184/93‑1 (MQ=255)

        agaATCTTCCAGTTGTACATCGTTCCACGTCGCTTCGACAACGGTATTATTCTCTGCCATTTTTACCGGTTTGGGTTCGGCTGGCGCTTTTTg                                                                                <  2:81654/93‑1 (MQ=255)

        agaATCTTCCAGTTGTACATCGTTCCACGTCGCTTCGACAACGGTATTATTCTCTGCCATTTTTACCGGTTTGGGTTCGGCTGGCGCTTTTTg                                                                                <  2:5158746/93‑1 (MQ=255)

         gaATCTTCCAGTTGTACATCGTTCCACGTCGCTTCGACAACGGTATTATTCTCTGCCATTTTTACCGGTTTGGGTTCGGCTGGCGCTTTTTGt                                                                               >  1:1338953/1‑93 (MQ=255)

         gaATCTTCCAGTTGTACATCGTTCCACGTCGCTTCGACAACGGTATTATTCTCTGCCATTTTTACCGGTTTGGGTTCGGCTGGCGCTTTTTGt                                                                               >  2:5365772/1‑93 (MQ=255)

         gaATCTTCCAGTTGTACATCGTTCCACGTCGCTTCGACAACGGTATTATTCTCTGCCATTTTTACCGGTTTGGATTCGGCTGGCGCTTTTTGt                                                                               <  2:6144227/93‑1 (MQ=255)

          aaTCTTCCAGTTGTACATCGTTCCACGTCGCTTCGACAACGGTATTATTCTCTGCCATTTTTACCGGTTTGGGTTCGGCTGGCGCTTTTTGtt                                                                              >  1:1158619/1‑93 (MQ=255)

            tCTTCCAGTTGTACATCGTTCCACTTCGCTTCGACAACGGTATTAGTCTCTGACATTTTTACCGGCTTGGGTTCGGCGGGCGCTTTTTTTTct                                                                            >  2:5872840/1‑92 (MQ=255)

            tCTTCCAGTTGTACATCGTTCCACGTCGCTTCGACAACGGTATTATTCTCTGCCATTTTTACCGGTTTGGGTTCGGCTGGCGCTTTTTGTTcg                                                                            >  2:2344714/1‑93 (MQ=255)

              ttCCAGTTGTACATCGTTCCACGTCGCTTCGACAACGGTATTATTCTCTGCCATTTTTACCGGTTTGGGTTCGGCTGGCGCTTTTTGTTcgcg                                                                          >  1:176849/1‑93 (MQ=255)

                ccAGTTGTACATCGTTCCACGTCGCTTCGACAACGGTATTATTCTCTGCCATTTTTACCGGTTTGGGTTCGGCTGGCGCTTTTTGTTCGCGTc                                                                        <  1:5060948/93‑1 (MQ=255)

                 cAGTTGTACATCGTTCCACGTCGCTTCGACAACGGTATTATTCTCTGCCATTTTTACCGGTTTGGGTTCGGCTGGCGCTTTTTGTTCGCGTcc                                                                       >  2:1428002/1‑93 (MQ=255)

                 cAGATGTACATCGTTCCACGTCGCTTCGACAACGGTATTATTCTCTGCCATTTTTACCGGTTTGGGTTCGGCTGGCGCTTTTTGTTCGCGTcc                                                                       <  2:2759763/93‑1 (MQ=255)

                  aGTTGTACATCGTTCCACGTCGCTTCGACAACGGTATTATTCTCTGCCATTTTTACCGGTTTGGGTTCGGCTGGCGCTTTTTGTTCGCGTCcg                                                                      >  2:5511547/1‑93 (MQ=255)

                      gTACATCGTTCCACGTCGCTTCGACAACGGTATTATTCTCTGCCATTTTTACCGGTTTGGGTTCGGCTGGCGCTTTTTGTTCGCGTCCGCGTa                                                                  <  1:1017448/93‑1 (MQ=255)

                       tACATCGTTCCACGTCGCTTCGACAACGGTATTATTCTCTGCCATTTTTACCGGTTTGGGTTCGGCTGGCGCTTTTTGTTCGCGTCCGCGTAt                                                                 <  2:2507389/93‑1 (MQ=255)

                       tACATCGTTCCACGTCGCTTCGACAACGGTATTATTCTCTGCCATTTTTACCGGTTTGGGTTCGGCTGGCGCTTTTTGTTCGCGTCCGCGTAt                                                                 <  1:2858331/93‑1 (MQ=255)

                        ccATCGTTCCACGTCGCTCCGACAACGGTATTATTCTCTGCCATTTTTCCCGGTTTGGGTTCGGCTGGCGCTTTTTGTTCGCGTCCGCGTATc                                                                <  2:4311607/92‑1 (MQ=255)

                        aCATCGTTCCACGTCGCTTCGACAACGGTATTATTCTCTGCCATTTTTACCGGTTTGGGTTCGGCTGGCGCTTTTTGTTCGCGTCCGCGTATc                                                                <  2:4394324/93‑1 (MQ=255)

                         cATCGTTCCACGTCGCTTCGACAACGGTATTATTCTCTGCCATTTTTACCGGTTTGGGTTCGGCTGGCGCTTTTTGTTCGCGTCCGCGCATcc                                                               <  2:2748452/93‑1 (MQ=255)

                          aTCGTTCCACGTCGCTTCGACAACGGTATTATTCTCTGCCATTTTTACCGGTTTGGGTTCGGCTGGCGCTTTTTGTTCGCGTCCGCGTATcca                                                              <  1:5780212/93‑1 (MQ=255)

                           tCGTTCCACGTCGCTTCGACAACGGTATTATTCTCTGCCATTTTTACCGGTTTGGGTTCGGCTGGCGCTTTTTGTTCGCGTCCGCGTATccac                                                             >  2:4642255/1‑93 (MQ=255)

                             gTTCCACGTCGCTTCGACAACGGTATTATTCTCTGCCATTTTTACCGGTTTGGGTTCGGCTGGCGCTTTTTGTTCGCGTCCGCGTATccacca                                                           <  2:5944689/93‑1 (MQ=255)

                             gTTCCACGTCGCTTCGACAACGGTATTATTCTCTGCCATTTTTACCGGTTTGGGTTCGGCTGGCGCTTTTTGTTCGCGTCCGCGTATccacca                                                           >  1:5001915/1‑93 (MQ=255)

                             gTTCCACGTCGCTTCGACAACGGTATTATTCTCTGCCATTTTTACCGGTTTGGGTTCGGCTGGCGCTTTTTGTTCGCGTCCGCGTATccacca                                                           >  2:3161023/1‑93 (MQ=255)

                              ttCCACGTCGCTTCGACAACGGTATTATTCTCTGCCATTTTTACCGGTTTGGGTTCGGCTGGCGCTTTTTGTTCGCGTCCGCGTATCCACCAg                                                          >  2:3430097/1‑93 (MQ=255)

                               tCCACGTCGCTTCGACAACGGTATTATTCTCTGCCATTTTTACCGGTTTGGGTTCGGCTGGCGCTTTTTGTTCGCGTCCGCGTATCCACCAgg                                                         <  2:5239899/93‑1 (MQ=255)

                               tCCACGTCGCTTCGACAACGGTATTATTCTCTGCCATTTTTACCGGTTTGGGTTCGGCTGGCGCGTTTTGTTCGCGTCCGCGTATCCACCAgg                                                         >  2:5042498/1‑93 (MQ=255)

                                 cACGTCGCTTCGACAACGGTATTATTCTCTGCCATTTTTACCGGTTTGGGTTCGGCTGGCGCTTTTTGTTCGCGTCCGCGTATCCACCAggtc                                                       <  1:3008207/93‑3 (MQ=255)

                                  aCGTCGCTTCGACAACGGTATTATTCTCTGCCATTTTTACCGGTTTGGGTTCGGCTGGCGCTTTTTGTTCGCGTCCGCGTATCCACCAGGTCa                                                      >  2:2909013/1‑93 (MQ=255)

                                  aCGTCGCTTCGACAACGGTATTATTCTCTGCCATTTTTACCGGTTTGGGTTCGGCTGGCGCTCTTTGGTTGCGTCCGCGCATCCACCAGGccc                                                      >  1:5359422/1‑92 (MQ=255)

                                   cGTCGCTTCGACAACGGTATTATTCTCTGCCATTTTTACCGGTTTGGGTTCGGCTGGCGCTTTTTGTTCGCGTCCGCGTATCCACCAGGTCAg                                                     <  1:2881979/93‑1 (MQ=255)

                                    gTCGCTTCGACAACGGTATTATTCTCTGCCATTTTTACCGGTTTGGGTTCGGCTGGCGCTTTTTGTTCGCGTCCGCGTATCCACCAGGTCAGc                                                    <  2:330120/93‑1 (MQ=255)

                                     tCGCTTCGACAACGGTATTATTCTCTGCCATTTTTACCGGTTTGGGTTCGGCTGGCGCTTTTTGTTCGCGTCCGCGTATCCACCAGGTCAGcc                                                   <  2:4255479/93‑1 (MQ=255)

                                     tCGCTTCGACAACGGTATTATTCTCTGCCATTTTTACCGGTTTGGGTTCGGCTGGCGCTTTTTGTTCGCGTCCGCGTATCCACCAGGTCAGcc                                                   >  1:2704454/1‑93 (MQ=255)

                                      cGCTTCGACAACGGTATTATTCTCTGCCATTTTTACCGGTTTGGGTTCGGCTGGCGCTTTTTGTTCGCGTCCGCGTATCCACCAGGTCAGccc                                                  <  2:1234973/93‑1 (MQ=255)

                                       gCTTCGACAACGGTACTATTCTCTGCCATTTTTACCGGTTTGGGTTCGGCTGGCGCTTTTTGTTCGCGTCCGCGTATCCACCAGGTCAGCCCg                                                 >  2:5726004/1‑93 (MQ=255)

                                        cTTCTACAACGGTATTATTCTCTGCCATTTTTACCGGTTTGGGTTCGGCTGGCGCTTTTTGTTCGCGTCCGCGTATCCACCAGGTCAGCCCGa                                                <  1:1074399/93‑1 (MQ=255)

                                          tCGACAACGGTATTATTCTCTGCCATTTTTACCGGTTTGGGTTCGGCTGGCGCTTTTTGTTCGCGTCCGCGTATCCACCAGGTCAGCCCGAGc                                              >  1:3337907/1‑93 (MQ=255)

                                          tCGACAACGGTATTATTCTCTGCCATTTTTACCGGTTTGGGTTCGGCTGGCGCTTTTTGTTCGCGTCCGCGTATCCACCAGGTCAGCCCGAGc                                              >  2:3835614/1‑93 (MQ=255)

                                             aCAACGGTATTATTCTCTGCCATTTTTACCGGTTTGGGTTCGGCTGGCGCTTTTTGTTCGCGTCCGCGTATCCACCAGGTCAGCCCGAGCAAt                                           >  1:5963308/1‑93 (MQ=255)

                                              cAACGGTATTATTCTCTGCCATTTTTACCGGTTTGGGTTCGGCTGGCGCTTTTTGTTCGCGTCCGCGTATCCACCAGGTCAGCCCGAGCAATc                                          <  1:687893/93‑1 (MQ=255)

                                              cAACGGTATTATTCTCTGCCATTTTTACCGGTTTGGGTTCGGCTGGCGCTTTTTGTTCGCGTCCGCGTATCCACCAGGTCAGCCCGAGCAATc                                          >  1:3691610/1‑93 (MQ=255)

                                               aaCGGTATTATTCTCTGCCATTTTTGCCGGTTTGGGTTCGGCTGGCGCTTTTTGTTCGCGTCCGCGTATCCACCAGGTCAGCCCGAGCAATcc                                         <  2:6115578/93‑1 (MQ=255)

                                                aCGGTATTATTCTCTGCCATTTTTACCGGTTTGGGTTCGGCTGGCGCTTTTTGTTCGCGTCCGCGTATCCACCAGGTCAGCCCGAGCAATCCg                                        >  2:1474563/1‑93 (MQ=255)

                                                aCGGTATTATTCTCTGCCATTTTTACCGGTTTGGGTTCGGCTGGCGCTTTTTGTTCGCGTCCGCGTATCCACCAGGTCAGCCCGAGCAATCCg                                        <  2:2678934/93‑1 (MQ=255)

                                                  ggTATTATTCTCTGCCATTTTTACCGGTTTGGGTTCGGCTGGCGCTTTTTGTTCGCGTCCGCGTATCCACCAGGTCAGCCCGAGCAATCCGGc                                      >  1:1425023/1‑93 (MQ=255)

                                                   gTATTATTCTCTGCCATTTTTACCGGTTTGGGTTCGGCTGGCGCTTTTTGTTCGCGTCCGCGTATCCACCAGGTCAGCCCGAGCAATCCGGCa                                     >  1:5696544/1‑93 (MQ=255)

                                                   gTATTATTCTCTGCCATTTTTACCGGTTTGGGTTCGGCTGGCGCTTTTTGTTCGCGTCCGCGTATCCACCAGGTCAGCCCGAGCAATCCGGCa                                     <  1:4876058/93‑1 (MQ=255)

                                                   gTATTATTCTCTGCCATTTTTACCGGTTTGGGTTCGGCTGGCGCTTTTTGTTCGCGTCCGCGTATCCACCAGGTCAGCCCGAGCAATCCGGCa                                     >  1:1645920/1‑93 (MQ=255)

                                                    tattatTCTCTGCCATTTTTACCGGTTTGGGTTCGGCTGGCGCTTTTTGTTCGCGTCCGCGTATCCACCAGGTCAGCCCGAGCAATCCGGCAg                                    >  1:2807263/1‑93 (MQ=255)

                                                       tattCTCTGCCATTTTTACCGGTTTGGGTTCGGCTGGCGCTTTTTGTTCGCGTCCGCGTATCCACCAGGTCAGCCCGAGCAATCCGGCAGTGa                                 <  1:206943/93‑1 (MQ=255)

                                                        attCTCTGCCATTTTTACCGGTTTGGGTTCGGCTGGCGCTTTTTGTTCGCGTCCGCGTATCCACCAGGTCAGCCCGAGCAATCCGGCAGTGaa                                <  1:5703729/93‑1 (MQ=255)

                                                         ttCTCTGCCATTTTTACCGGTTTGGGTTCGGCTGGCGCTTTTTGTTCGCGTCCGCGTATCCACCAGGTCAGCCCGAGCAATCCGGCAGTGAAc                               >  1:6082081/1‑93 (MQ=255)

                                                          tctcTGCCATTTTTACCGGTTTGGGTTCGGCTGGCGCTTTTTGTTCGCGTCCGCGTATCCACCAGGTCAGCCCGAGCAATCCGGCAGTGAACa                              <  1:3279596/93‑1 (MQ=255)

                                                            tctGCCATTTTTACCGGTTTGGGATCCGCTGGCGCTTTTTGCTCGCGTCCGCGTATCCACCCGGGCAGGCCGAGCAATGCGGCCATGAACAgt                            >  2:1024153/1‑92 (MQ=255)

                                                             ctGCCTTTTTTACCGGTTTGGGTTCGGCTGGCGCTTTTTGTTCGCGGCCGAGTATCCACCAGCCCAGCCCGCGCACTCCGGCAGTGAACAGCa                           <  2:161865/93‑1 (MQ=255)

                                                              tGCCATTTTTACCGGTTTGGGTTCGGCTGGCGCTTTTTGTTCGCGTCCGCGTATCCACCAGGTCAGCCCGAGCAATCCGGCAGTGAACAGCaa                          >  1:4619537/1‑93 (MQ=255)

                                                               gCCATTTTTACCGGTTTGGGTTCGGCTGGCGCTTTTTGTTCGCGTCCGCGTATCCACCAGGTCAGCCCGAGCAATCCGGCAGTGAACAGCaaa                         >  2:189872/1‑93 (MQ=255)

                                                                ccATTTTTACCGGTTTGGGTTCGGCTGGCGCTTTTTGTTCGCGTCCGCGTATCCACCAGGTCAGCCCGAGCAATCCGGCAGTGAACAGCaaaa                        >  1:70554/1‑93 (MQ=255)

                                                                 cATTTTTACCGGTTTGGGTTCGGCTGGCGCTTTTTGTTCGTGTCCGCGTATCCACCCGGTCAGGCCGAGCAATCCGGCAGTGGACAGCaaaaa                       >  1:2346087/1‑93 (MQ=255)

                                                                 cATTTTTACCGGTTTGGGTTCGGCTGGCGCTTTTTGTTCGCGTCCGCGTATCCACCAGGTCAGCCCGAGCAATCCGGCAGTGAACAGCaaaaa                       <  1:2531553/93‑1 (MQ=255)

                                                                 cATTTTTACCGGTTTGGGTTCGGCTGGCGCTTTTTGTTCGCGTCCGCGTATCCACCAGGTCAGCCCGAGCAATCCGGCAGTGAACAGCaaaaa                       >  1:461844/1‑93 (MQ=255)

                                                                 cATTTTTACCGGTTTGGGTTCGGCTGGCGCTTTTTGTTCGCGTCCGCGTATCCACCAGGTCAGCCCGAGCAATCCGGCAGTGAACAGCaaaaa                       >  1:6217268/1‑93 (MQ=255)

                                                                  aTTTTTACCGGTTTGGGTTCGGCTGGCGCTTTTTGTTCGCGTCCGCGTATCCACCAGGTCAGCCCGAGCAATCCGGCAGTGAACAGCAAAAAt                      >  2:2833670/1‑93 (MQ=255)

                                                                    ttttACCGGTTTGGGTTCGGCTGGCGCTTTTTGTTCGCGTCCGCGTATCCACCAGGTCAGCCCGAGCAATCCGGCAGTGAACAGCAAAAATAc                    <  2:4807758/93‑1 (MQ=255)

                                                                     tttACCGGTTTGGGTTCGGCTGGCGCTTTTTGTTCGCGTCCGCGTATCCACCAGGTCAGCCCGAGCAATCCGGCAGTGAACAGCAAAAATAcc                   <  2:3427203/93‑1 (MQ=255)

                                                                       tACCGGTTTGGGTTCGGCTGGCGCTTTTTGTTCGCGTCCGCGTATCCACCAGGTCAGCCCGAGCAATCCGGCAGTGAACAGCAAAAATACCAg                 <  2:1656134/93‑1 (MQ=255)

                                                                       tACCGGTTTGGGTTCGGCTGGCGCTTTTTGTTCGCGTCCGCGTATCCACCAGGTCAGCCCGAGCAATCCGGCAGTGAACAGCAAAAATACCAg                 >  1:5818598/1‑93 (MQ=255)

                                                                        aCCGGTTTGGGTTCGGCTGGCGCTTTTTGTTCGCGTCCGCGTATCCACCAGGTCAGCCCGAGCAATCCGGCAGTGAACAGCAAAAATACCAgg                <  2:199587/93‑1 (MQ=255)

                                                                        aCCGGTTTGGGTTCGGCTGGCGCTTTTTGTTCGCGTCCGCGTATCCACCAGGTCAGCCCGAGCAATCCGGCAGTGAACAGCAAAAATACCAgg                <  2:6070940/93‑1 (MQ=255)

                                                                         ccGGTTTGGGTTCGGCTGGCGCTTTTTGTTCGCGTCCGCGTATCCACCAGGTCAGCCCGAGCAATCCGGCAGTGAACAGCAAAAATACCAGGt               <  1:2504214/93‑1 (MQ=255)

                                                                           ggTTTGGGTTCGGCTGGCGCTTTTTGTTCGCGTCCGCGTATCCACCAGGTCAGCCCGAGCAATCCGGCAGTGAACAGCAAAAATACCAGGTTc             >  2:5317935/1‑93 (MQ=255)

                                                                            gTTTGGGTTCGGCTGGCGCTTTTTGTTCGCGTCCGCGTATCCACCAGGTCAGCCCGAGCAATCCGGCAGTGAACAGCAAAAATACCAGGTTCg            >  2:1408276/1‑93 (MQ=255)

                                                                            gTTTGGGTTCGGCTGGCGCTTTTTGTTCGCGTCCGCGTATCCACCAGGTCAGCCCGAGCAATCCGGCAGTGAACAGCAAAAATACCAGGTTCg            >  2:1128074/1‑93 (MQ=255)

                                                                             tttGGGTTCGGCTGGCGCTTTTTGTTCGCGTCCGCGTATCCACCAGGTCAGCCCGAGCAATCCGGCAGTGAACAGCAAAAATACCAGGTTggg           >  1:1933632/1‑93 (MQ=255)

                                                                              ttGGGTTCGGCTGGCGCTTTTTGTTCGCGTCCGCGTATCCACCAGGTCAGCCCGAGCAATCCGGCAGTGAACAGCAAAAATACCAGGTTCGGc          <  2:72819/93‑1 (MQ=255)

                                                                               tGGGTTCGGCTGGCGCTTTTTGTTCGCGTCCGCGTATCCACCAGGTCAGCCCGAGCAATCCGGCAGTGAACAGCAAAAATACCAGGTTCGGCa         >  1:1864837/1‑93 (MQ=255)

                                                                                gggTTCGGCTGGCGCTTTTTGTTCGCGTCCGCGTATCCACCAGGTCAGCCCGAGCAATCCGGCAGTGAACAGCAAAAATACCAGGTTCGGCAt        <  1:423834/93‑1 (MQ=255)

                                                                                gggTTCGGCTGGCGCTTTTTGTTCGCGTCCGCGTATCCACCAGGTCAGCCCGAGCAATCCGGCAGTGAACAGCAAAAATACCAGGTTCGGCAt        <  1:2553833/93‑1 (MQ=255)

                                                                                 ggTTCGGCTGGCGCTTTTTGTTCGCGTCCGCGTATCCACCAGGTCAGCCCGAGCAATCCGGCAGTGAACAGCAAAAATACCAGGTTCGGCAtt       >  1:313583/1‑93 (MQ=255)

                                                                                  gTTCGGCTGGCGCTTTTTGTTCGCGTCCGCGTATCCACCAGGTCAGCCCGAGCAATCCGGCAGTGAACAGCAAAAATACCAGGTTCGGCATTc      >  1:5595718/1‑93 (MQ=255)

                                                                                   ttCGGCTGGCGCTTTTTGTTCGCGTCCGCGTATCCACCAGGTCAGCCCGAGCAATCCGGCAGTGAACAGCAAAAATACCAGGTTCGGCATTcc     <  2:3691610/93‑1 (MQ=255)

                                                                                   ttCGGCTGGCGCTTTTTGTTCGCGTCCGCGTATCCACCAGGTCAGCCCGAGCAATCCGGCAGTGAACAGCAAAAATACCAGGTTCGGCATTcc     <  2:8049/93‑1 (MQ=255)

                                                                                    tCGGCTGGCGCTTTTTGTTCGCGTCCGCGTATCCACCAGGTCAGCCCGAGCAATCCGGCAGTGAACAGCAAAAATACCAGGTTCGGCATTCCa    <  1:5542942/93‑1 (MQ=255)

                                                                                    tCGGCTGGCGCTTTTTGTTCGCGTCCGCGTATCCACCAGGTCAGCCCGAGCAATCCGGCAGTGAACAGCAAAAATACCAGGTTCGGCATTCCa    <  2:3925018/93‑1 (MQ=255)

                                                                                     cGGCTGGCGCTTTTTGTTCGCGTCCGCGTATCCACCAGGTCAGCCCGAGCAATCCGGCAGTGAACAGCAAAAATACCAGGTTCGGCATTCCAg   <  1:5768262/93‑1 (MQ=255)

                                                                                     cGGCTGGCGCTTTTTGTTCGCGTCCGCGTATCCACCAGGTCAGCCCGAGCAATCCGGCAGTGAACAGCAAAAATACCAGGTTCGGCATTCCAg   <  2:3894864/93‑1 (MQ=255)

                                                                                     cGGCTGGCGCTTTTTGTTCGCGTCCGCGTATCCACCAGGTCAGCCCGAGCAATCCGGCAGTGAACAGCAAAAATACCAGGTTCGGCATTCCAg   <  1:3925533/93‑1 (MQ=255)

ATTCCCAGAGAATCTTCCAGTTGTACATCGTTCCACGTCGCTTCGACAACGGTATTATTCTCTGCCATTTTTACCGGTTTGGGTTCGGCAGGCGCTTTTTGTTCGCGTCCGCGTATCCACCAGGCCAGCCCGAGCAATCCGGCAGTGAACAGCAAAAATACCAGGTTCGGCATTCCAGG  >  NC_000913/1961981‑1962159

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

TTCCATTCCCAGAGAATCTTCCAGTTGTACATCGTTCCACGTCGCTTCGACAACGGTATTATTCTCTGCCATTTTTACCGGTTTGGGTTCGGCAGGCGCTTTTTGTTCGCGTCCGCGTATCCACCAGGCCAGCCCGAGCAATCCGGCAGTGAACAGCAAAAATACCAGGTTCGGCATTCCAGGCACC  >  NC_000913/1961977‑1962163

TTCCATTCCCAGAGAATCTTCCAGTTGTACATCGTTCCACGTCGCTTCGACAACGGTATTATTCTCTGCCATTTTTACCGGTTTGGGTTCGGCT                                                                                               >  SRR4302533.6026280/1‑94 (MQ=60)

 tcatcaaaattttttttttcttttcttccgccccccccttcttctaaaacattgttttttTTCTCTGCCATTTTTACCGGTTTGGGTTCGGCTG                                                                                              <  SRR4302533.5911291/34‑1 (MQ=60)

  CCATTCCCAGAGAATCTTCCAGTTGTACATCGTTCCACGTCGCTTCGACAACGGTATTATTCTCTGCCATTTTTACCGGTTTGGGTTCGGCTGG                                                                                             <  SRR4302533.3577493/94‑1 (MQ=60)

  CCATTCCCAGAGAATCTTCCAGTTGTACATCGTTCCACGTCGCTTCGACAACGGTATTATTCTCTGCCATTTTTACCGGTTTGGGTTCGGCTGG                                                                                             <  SRR4302533.4316777/94‑1 (MQ=60)

    ATTCCCAGAGAATCTTCCAGTTGTACATCGTTCCACGTCGCTTCGACAACGGTATTATTCTCTGCCATTTTTACCGGTTTGGGTTCGGCTGGCG                                                                                           >  SRR4302533.2442800/1‑94 (MQ=60)

    ATTCCCAGAGAATCTTCCAGTTGTACATCGTTCCACGTCGCTTCGACAACGGTATTATTCTCTGCCATTTTTACCGGTTTGGGTTCGGCTGGCG                                                                                           >  SRR4302533.2738072/1‑94 (MQ=60)

    ATTCCCAGAGAATCTTCCAGTTGTACATCGTTCCACGTCGCTTCGACAACGGTATTATTCTCTGCCATTTTTACCGGTTTGGGTTCGGCTGGCG                                                                                           >  SRR4302533.4336187/1‑94 (MQ=60)

    ATTCCCAGAGAATCTTCCAGTTGTACATCGTTCCACGTCGCTTCGACAACGGTATTATTCTCTGCCATTTTTACCGGTTTGGGTTCGGCTGGCG                                                                                           >  SRR4302533.1187885/1‑94 (MQ=60)

     TTCCCAGAGAATCTTCCAGTTGTACATCGTTCCACGTCGCTTCGACAACGGTATTATTCTCTGCCATTTTTACCGGTTTGGGTTCGGCTGGCGC                                                                                          >  SRR4302533.1017136/1‑94 (MQ=60)

      TCCCAGAGAATCTTCCAGTTGTACATCGTTCCACGTCGCTTCGACAACGGTATTATTCTCTGCCATTTTTACCGGTTTGGGTTCGGCTGGCGCT                                                                                         >  SRR4302533.808075/1‑94 (MQ=60)

        CCAGAGAATCTTCCAGTTGTACATCGTTCCACGTCGCTTCGACAACGGTATTATTCTCTGCCATTTTTACCGGTTTGGGTTCGGCTGGCGCTTT                                                                                       >  SRR4302533.6107671/1‑94 (MQ=60)

         CAGAGAATCTTCCAGTTGTACATCGTTCCACGTCGCTTCGACAACGGTATTATTCTCTGCCATTTTTACCGGTTTGGGTTCGGCTGGCGCTTTT                                                                                      <  SRR4302533.863442/94‑1 (MQ=60)

           GAGAATCTTCCAGTTGTACATCGTTCCACGTCGCTTCGACAACGGTATTATTCTCTGCCATTTTTACCGGTTTGGGTTCGGCTGGCGCTTTTTG                                                                                    >  SRR4302533.73287/1‑94 (MQ=60)

            AGAATCTTCCAGTTGTACATCGTTCCACGTCGCTTCGACAACGGTATTATTCTCTGCCATTTTTACCGGTTTGGGTTCGGCTGGCGCTTTTTGT                                                                                   >  SRR4302533.1347013/1‑94 (MQ=60)

             GAATCTTCCAGTTGTACATCGTTCCACGTCGCTTCGACAACGGTATTATTCTCTGCCATTTTTACCGGTTTGGGTTCGGCTGGCGCTTTTTGTT                                                                                  >  SRR4302533.1165572/1‑94 (MQ=60)

              AATCTTCCAGTTGTACATCGTTCCACGTCGCTTCGACAACGGTATTATTCTCTGCCATTTTTACCGGTTTGGGTTCGGCTGGCGCTTTTTGTTC                                                                                 >  SRR4302533.1413482/1‑94 (MQ=60)

              AATCTTCCAGTTGTACATCGTTCCACGTCGCTTCGACAACGGTATTATTCTCTGCCATTTTTACCGGTTTGGGTTCGGCTGGCGCTTTTTGTTC                                                                                 <  SRR4302533.5660557/94‑1 (MQ=60)

               ATCTTCCAGTTGTACATCGTTCCACGTCGCTTCGACAACGGTATTATTCTCTGCCATTTTTACCGGTTTGGGTTCGGCTGGCGCTTTTTGTTCG                                                                                <  SRR4302533.6042935/94‑1 (MQ=60)

               ATCTTCCAGTTGTACATCGTTCCACGTCGCTTCGACAACGGTATTATTCTCTGCCATTTTTACCGGTTTGGGTTCGGCTGGCGCTTTTTGTTCG                                                                                <  SRR4302533.1978493/94‑1 (MQ=60)

                 CTTCCAGTTGTACATCGTTCCACGTCGCTTCGACAACGGTATTATTCTCTGCCATTTTTACCGGTTTGGGTTCGGCTGGCGCTTTTTGTTCGCG                                                                              >  SRR4302533.177959/1‑94 (MQ=60)

                   TCCAGTTGTACATCGTTCCACGTCGCTTCGACAACGGTATTATTCTCTGCCATTTTTACCGGTTTGGGTTCGGCTGGCGCTTTTTGTTCGCGTC                                                                            <  SRR4302533.5588856/94‑1 (MQ=60)

                    CCAGTTGTACATCGTTCCACGTCGCTTCGACAACGGTATTATTCTCTGCCATTTTTACCGGTTTGGGTTCGGCTGGCGCTTTTTGTTCGCGTCC                                                                           <  SRR4302533.5091531/94‑1 (MQ=60)

                       GTTGTACATCGTTCCACGTCGCTTCGACAACGGTATTATTCTCTGCCATTTTTACCGGTTTGGGTTCGGCTGGCGCTTTTTGTTCGCGTCCGCG                                                                        <  SRR4302533.2658850/94‑1 (MQ=60)

                         TGTACATCGTTCCACGTCGCTTCGACAACGGTATTATTCTCTGCCATTTTTACCGGTTTGGGTTCGGCTGGCGCTTTTTGTTCGCGTCCGCGTT                                                                      >  SRR4302533.1180829/1‑94 (MQ=60)

                          GTACATCGTTCCACGTCGCTTCGACAACGGTATTATTCTCTGCCATTTTTACCGGTTTGGGTTCGGCTGGCGCTTTTTGTTCGCGTCCGCGTAT                                                                     <  SRR4302533.1023429/94‑1 (MQ=60)

                           TACATCGTTCCACGTCGCTTCGACAACGGTATTATTCTCTGCCATTTTTACCGGTTTGGGTTCGGCTGGCGCTTTTTGTTCGCGTCCGCGTATC                                                                    <  SRR4302533.2875916/94‑1 (MQ=60)

                             CATCGTTCCACGTCGCTTCGACAACGGTATTATTCTCTGCCATTTTTACCGGTTTGGGTTCGGCTGGCGCTTTTTGTTCGCGTCCGCGTATCCA                                                                  <  SRR4302533.1151894/94‑1 (MQ=60)

                              ATCGTTCCACGTCGCTTCGACAACGGTATTATTCTCTGCCATTTTTACCGGTTTGGGTTCGGCTGGCGCTTTTTGTTCGCGTCCGCGTATCCAC                                                                 <  SRR4302533.5815123/94‑1 (MQ=60)

                               TCGTTCCACGTCGCTTCGACAACGGTATTATTCTCTGCCATTTTTACCGGTTTGGGTTCGGCTGGCGCTTTTTGTTCGCGTCCGCGTATCCACC                                                                >  SRR4302533.3000967/1‑94 (MQ=60)

                                CGTTCCACGTCGCTTCGACAACGGTATTATTCTCTGCCATTTTTACCGGTTTGGGTTCGGCTGGCGCTTTTTGTTCGCGTCCGCGTATCCACCA                                                               >  SRR4302533.5857082/1‑94 (MQ=60)

                                CGTTCCACGTCGCTTCGACAACGGTATTATTCTCTGCCATTTTTACCGGTTTGGGTTCGGCTGGCGCTTTTTGTTCGCGTCCGCGTATCCACCA                                                               >  SRR4302533.5032236/1‑94 (MQ=60)

                                 GTTCCACGTCGCTTCGACAACGGTATTATTCTCTGCCATTTTTACCGGTTTGGGTTCGGCTGGCGCTTTTTGTTCGCGTCCGCGTATCCACCAG                                                              >  SRR4302533.1933037/1‑94 (MQ=60)

                                    CCACGTCGCTTCGACAACGGTATTATTCTCTGCCATTTTTACCGGTTTGGGTTCGGCTGGCGCTTTTTGTTCGCGTCCGCGTATCCACCAGGTC                                                           <  SRR4302533.5055917/94‑1 (MQ=60)

                                     CACGTCGCTTCGACAACGGTATTATTCTCTGCCATTTTTACCGGTTTGGGTTCGGCTGGCGCTTTTTGTTCGCGTCCGCGTATCCACCAGGTCA                                                          <  SRR4302533.1862725/94‑1 (MQ=60)

                                     CACGTCGCTTCGACAACGGTATTATTCTCTGCCATTTTTACCGGTTTGGGTTCGGCTGGCGCTTTTTGTTCGCGTCCGCGTATCCACCAGGTCA                                                          <  SRR4302533.3026611/94‑1 (MQ=60)

                                     CACGTCGCTTCGACAACGGTATTATTCTCTGCCATTTTTACCGGTTTGGGTTCGGCTGGCGCTCTTTGGTTGCGTCCGCGCATCCACCAGGCCC                                                          >  SRR4302533.5391605/1‑94 (MQ=60)

                                     CACGTCGCTTCGACAACGGTATTATTCTCTGCCATTTTTACCGGTTTGGGTTCGGCGGGCGCTTTTTGTTCGCGTCCGCGTATCCACCAGGTCA                                                          <  SRR4302533.3081309/94‑1 (MQ=60)

                                       CGTCGCTTCGACAACGGTATTATTCTCTGCCATTTTTACCGGTTTGGGTTCGGCTGGCGCTTTTTGTTCGCGTCCGCGTATCCACCAGGTCAGC                                                        >  SRR4302533.1026817/1‑94 (MQ=60)

                                       CGTCGCTTCGACAACGGTATTATTCTCTGCCATTTTTACCGGTTTGGGTTCGGCTGGCGCTTTTTGTTCGCGTCCGCGTATCCACCAGGTCAGC                                                        <  SRR4302533.2899709/94‑1 (MQ=60)

                                        GTCGCTTCGACAACGGTATTATTCTCTGCCATTTTTACCGGTTTGGGTTCGGCTGGCGCTTTTTGTTCGCGTCCGCGTATCCACCAGGTCAGCC                                                       <  SRR4302533.236825/94‑1 (MQ=60)

                                        GTCGCTTCGACAACGGTATTATTCTCTGCCATTTTTACCGGTTTGGGTTCGGCTGGCGCTTTTTGTTCGCGTCCGCGTATCCACCAGGTCAGCC                                                       >  SRR4302533.2721044/1‑94 (MQ=60)

                                            CTTCTACAACGGTATTATTCTCTGCCATTTTTACCGGTTTGGGTTCGGCTGGCGCTTTTTGTTCGCGTCCGCGTATCCACCAGGTCAGCCCGAG                                                   <  SRR4302533.1080732/94‑1 (MQ=60)

                                            CTTCGACAACGGTATTATTCTCTGCCATTTTTACCGGTTTGGGTTCGGCTGGCGCTTTTTGTTCGCGTCCGCGTATCCACCAGGTCAGCCCGAG                                                   >  SRR4302533.3328671/1‑94 (MQ=60)

                                             TTCGACAACGGTATTATTCTCTGCCATTTTTACCGGTTTGGGTTCGGCTGGCGCTTTTTGTTCGCGTCCGCGTATCCACCAGGTCAGCCCGAGC                                                  >  SRR4302533.3358538/1‑94 (MQ=60)

                                                GACAACGGTATTATTCTCTGCCATTTTTACCGGTTTGGGTTCGGCTGGCGCTTTTTGTTCGCGTCCGCGTATCCACCAGGTCAGCCCGAGCAAT                                               >  SRR4302533.5999384/1‑94 (MQ=60)

                                                 ACAACGGTATTATTCTCTGCCATTTTTACCGGTTTGGGTTCGGCTGGCGCTTTTTGTTCGCGTCCGCGTATCCACCAGGTCAGCCCGAGCAATC                                              >  SRR4302533.5741626/1‑94 (MQ=60)

                                                 ACAACGGTATTATTCTCTGCCATTTTTACCGGTTTGGGTTCGGCTGGCGCTTTTTGTTCGCGTCCGCGTATCCACCAGGTCAGCCCGAGCAATC                                              >  SRR4302533.3714291/1‑94 (MQ=60)

                                                  CAACGGTATTATTCTCTGCCATTTTTACCGGTTTGGGTTCGGCTGGCGCTTTTTGTTCGCGTCCGCGTATCCACCAGGTCAGCCCGAGCAATCC                                             <  SRR4302533.691961/94‑1 (MQ=60)

                                                   AACGGTATTATTCTCTGCCATTTTTACCGGTTTGGGTTCGGCTGGCGCTTTTTGTTCCCGTCCGCGTATCCCGCAGGTCAGCCCGCGCAATCCG                                            >  SRR4302533.5989151/1‑94 (MQ=60)

                                                     CGGTATTATTCTCTGCCATTTTTACCGGTTTGGGTTCGGCTGGCGCTTTTTGTTCGCGTCCGCGTATCCACCAGGTCAGCCCGAGCAATCCGGC                                          >  SRR4302533.1433642/1‑94 (MQ=60)

                                                      GGTATTATTCTCTGCCATTTTTACCGGTTTGGGTTCGGCTGGCGCTTTTTGTTCGCGTCCGCGTATCCACCAGGTCAGCCCGAGCAATCCGGCA                                         <  SRR4302533.5810716/94‑1 (MQ=60)

                                                      GGTATTATTCTCTGCCATTTTTACCGGTTTGGGTTCGGCTGGCGCTTTTTGTTCGCGTCCGCGTATCCACCAGGTCAGCCCGAGCAATCCGGCA                                         >  SRR4302533.1655884/1‑94 (MQ=60)

                                                      GGTATTATTCTCTGCCATTTTTACCGGTTTGGGTTCGGCTGGCGCTTTTTGTTCGCGTCCGCGTATCCACCAGGTCAGCCCGAGCAATCCGGCA                                         >  SRR4302533.5730957/1‑94 (MQ=60)

                                                       GTATTATTCTCTGCCATTTTTACCGGTTTGGGTTCGGCTGGCGCTTTTTGTTCGCGTCCGCGTATCCACCAGGTCAGCCCGAGCAATCCGGCAG                                        >  SRR4302533.2824538/1‑94 (MQ=60)

                                                       GTATTATTCTCTGCCATTTTTACCGGTTTGGGTTCGGCTGGCGCTTTTTGTTCGCGTCCGCGTATCCACCAGGTCAGCCCGAGCAATCCGGCAG                                        <  SRR4302533.4905601/94‑1 (MQ=60)

                                                         ATTATTCTCTGCCATTTTTACCGGTTTGGGTTCGGCTGGCGCTTTTTGTTCGCGTCCGCGTATCCACCAGGTCAGCCCGAGCAATCCGGCAGTG                                      >  SRR4302533.3699716/1‑94 (MQ=60)

                                                          TTATTCTCTGCCATTTTTACCGGTTTGGGTTCGGCTGGCGCTTTTTGTTCGCGTCCGCGTATCCACCAGGTCAGCCCGAGCAATCCGGCAGTGA                                     >  SRR4302533.1661286/1‑94 (MQ=60)

                                                          TTATTCTCTGCCATTTTTACCGGTTTGGGTTCGGCTGGCGCTTTTTGTTCGCGTCCGCGTATCCACCAGGTCAGCCCGAGCAATCCGGCAGTGA                                     <  SRR4302533.2831999/94‑1 (MQ=60)

                                                           TATTCTCTGCCATTTTTACCGGTTTGGGTTCGGCTGGCGCTTTTTGTTCGCGTCCGCGTATCCACCAGGTCAGCCCGAGCAATCCGGCAGTGAA                                    <  SRR4302533.208255/94‑1 (MQ=60)

                                                           TATTCTCTGCCATTTTTACCGGTTTGGGTTCGGCTGGCGCTTTTTGTTCGCGTCCGCGTATCCACCAGGTCAGCCCGAGCAATCCGGCAGTGAA                                    >  SRR4302533.1443984/1‑94 (MQ=60)

                                                           TATTCTCTGCCATTTTTACCGGTTTGGGTTCGGCTGGCGCTTTTTGTTCGCGTCCGCGTATCCACCAGGTCAGCCCGAGCAATCCGGCAGTGAA                                    <  SRR4302533.1283225/94‑1 (MQ=60)

                                                            ATTCTCTGCCATTTTTACCGGTTTGGGTTCGGCTGGCGCTTTTTGTTCGCGTCCGCGTATCCACCAGGTCAGCCCGAGCAATCCGGCAGTGAAC                                   <  SRR4302533.5738182/94‑1 (MQ=60)

                                                            ATTCTCTGCCATTTTTACCGGTTTGGGTTCGGCTGGCGCTTTTTGTTCGCGTCCGCGTATCCACCAGGTCAGCCCGAGCAATCCGGCAGTGAAC                                   >  SRR4302533.6118858/1‑94 (MQ=60)

                                                            ATTCTCTGCCATTTTTACCGGTTTGGGTTCGGCTGGCGCTTTTTGTTCGCGTCCGCGTATCCACCAGGTCAGCCCGAGCAATCCGGCAGTGAAC                                   <  SRR4302533.687145/94‑1 (MQ=60)

                                                              TCTCTGCCATTTTTACCGGTTTGGGTTCGGCTGGCGCTTTTTGTTCGCGTCCGCGTATCCACCAGGTCAGCCCGAGCAATCCGGCAGTGAACAG                                 >  SRR4302533.5165280/1‑94 (MQ=60)

                                                              TCTCTGCCATTTTTACCGGTTTGGGTTCGGCTGGCGCTTTTTGTTCGCGTCCGCGTATCCACCAGGTCAGCCCGAGCAATCCGGCAGTGAACAG                                 <  SRR4302533.3299842/94‑1 (MQ=60)

                                                               CTCTGCCATTTTTACCGGTTTGGGTTCGGCTGGCGCTTTTTGTTCGCGTTCGCGTATCCACCAGGTCAGCCCGAGCAATCCGGCAGTGCACAGC                                >  SRR4302533.4989648/1‑94 (MQ=60)

                                                                 CTGCCATTTTTACCGGTTTGGGTTCGGCTGGCGCTTTTTGTTCGCGTCCGCGTATCCACCAGGTCAGCCCGAGCAATCCGGCAGTGAACAGCAA                              >  SRR4302533.4647531/1‑94 (MQ=60)

                                                                  TGCCATTTTTACCGGTTTGGGTTCGGCTGGCGCTTTTTGTTCGCGTCCGCGTATCCACCAGGTCAGCCCGAGCAATCCGGCAGTGAACAGCAAA                             >  SRR4302533.2551649/1‑94 (MQ=60)

                                                                   GCCATTTTTACCGGTTTGGGTTCGGCTGGCGCTTTTTGTTCGCGTCCGCGTATCCACCAGGTCAGCCCGAGCAATCCGGCAGTGAACAGCAAAA                            >  SRR4302533.71011/1‑94 (MQ=60)

                                                                   GCCATTTTTACCGGTTTGGGTTCGGCTGGCGCTTTTTGGTCGCGTCCGCGTATGCACCAGGTCAGCACGAGCAATCCGGCAGTGAACAGCAAAA                            >  SRR4302533.5943489/1‑94 (MQ=60)

                                                                    CCATTTTTACCGGTTTGGGTTCGGCTGGCGCTTTTTGTTCGTGTCCGCGTATCCACCCGGTCAGGCCGAGCAATCCGGCAGTGGACAGCAAAAA                           >  SRR4302533.2360608/1‑94 (MQ=60)

                                                                    CCATTTTTACCGGTTTGGGTTCGGCTGGCGCTTTTTGTTCGCGTCCGCGTATCCACCAGGTCAGCCCGAGCAATCCGGCAGTGAACAGCAAAAA                           >  SRR4302533.6254547/1‑94 (MQ=60)

                                                                    CCATTTTTACCGGTTTGGGTTCGGCTGGCGCTTTTTGTTCGCGTCCGCGTATCCACCAGGTCAGCCCGAGCAATCCGGCAGTGAACAGCAAAAA                           >  SRR4302533.464599/1‑94 (MQ=60)

                                                                     CATTTTTACCGGTTTGGGTTCGGCTGGCGCTTTTTGTTCGCGTCCGCGTATCCACCAGGTCAGCCCGAGCAATCCGGCAGTGAACAGCAAAAAT                          <  SRR4302533.2474481/94‑1 (MQ=60)

                                                                     CATTTTTACCGGTTTGGGTTCGGCTGGCGCTTTTTGTTCGCGTCCGCGTATCCACCAGGTCAGCCCGAGCAATCCGGCAGTGAACAGCAAAAAT                          <  SRR4302533.2547137/94‑1 (MQ=60)

                                                                       TTTTTACCGGTTTGGGTTCGGCTGGCGCTTTTTGTTCGCGTCCGCGTATCCACCAGGTCAGCCCGAGCAATCCGGCAGTGAACAGCAAAAATAC                        <  SRR4302533.328918/94‑1 (MQ=60)

                                                                          TTACCGGTTTGGGTTCGGCTGGCGCTTTTTGTTCGCGTCCGCGTATCCACCAGGTCAGCCCGAGCAATCCGGCAGTGCACAGCAAAAATACCAG                     >  SRR4302533.6150864/1‑94 (MQ=60)

                                                                          TTACCGGTTTGGGTTCGGCTGGCGCTTTTTGTTCGCGTCCGCGTATCCACCAGGTCAGCCCGAGCAATCCGGCAGTGAACAGCAAAAATACCAG                     >  SRR4302533.5853746/1‑94 (MQ=60)

                                                                           TACCGGTTTGGGTTCGGCTGGCGCTTTTTGTTCGCGTCCGCGTATCCACCAGGTCAGCCCGAGCAATCCGGCAGTGAACAGCAAAAATACCAGG                    <  SRR4302533.3826867/94‑1 (MQ=60)

                                                                           TACCGGTTTGGGTTCGGCTGGCGCTTTTTGTTCGCGTCCGCGTATCCACCAGGTCAGCCCGAGCAATCCGGCAGTGAACAGCAAAAATACCAGG                    >  SRR4302533.4673105/1‑94 (MQ=60)

                                                                            ACCGGTTTGGGTTCGGCTCGCGCTTTTTGTCCGCGTCCGCGTATCCACCAGGTCAGCCCGAGCAATCCGGCAGTGAACAGCAAAAATACCAGGT                   <  SRR4302533.2091341/94‑1 (MQ=60)

                                                                             CCGGTTTGGGTTCGGCTGGCGCTTTTTGTTCGCGTCCGCGTATCCACCAGGTCAGCCCGAGCAATCCGGCAGTGAACAGCAAAAATACCAGGTT                  >  SRR4302533.704428/1‑94 (MQ=60)

                                                                             CCGGTTTGGGTTCGGCTGGCGCTTTTTGTTCGCGTCCGCGTATCCACCAGGTCAGCCCGAGCAATCCGGCAGTGAACAGCAAAAATACCAGGTT                  <  SRR4302533.2519654/94‑1 (MQ=60)

                                                                                GTTTGGGTTCGGCTGGCGCTTTTTGTTCGCGTCCGCGTATCCACCAGGTCAGCCCGAGCAATCCGGCAGTGAACAGCAAAAATACCAGGTTGGG               >  SRR4302533.1945431/1‑94 (MQ=60)

                                                                                GTTTGGGTTCGGCTGGCGCTTTTTGTTCGCGTCCGCGTATCCACCAGGTCAGCCCGAGCAATCCGGCAGTGAACAGCAAAAATACCAGGTTCGG               <  SRR4302533.2892868/94‑1 (MQ=60)

                                                                                GTTTGGGTTCGGCTGGCGCTTTTTGTTCGCGTCCGCGTATCCACCAGGTCAGCCCGAGCAATCCGGCAGTGAACAGCAAAAATACCAGGTTCGG               <  SRR4302533.4217755/94‑1 (MQ=60)

                                                                                 TTTGGGTTCGGCTGGCGCTTTTTGTTCGCGTCCGCGTATCCACCAGGTCAGCCCGAGCAATCCGGCAGTGAACAGCAAAAATACCAGGTTCGGC              >  SRR4302533.573154/1‑94 (MQ=60)

                                                                                  TTGGGTTCGGCTGGCGCTTTTTGTTCGCGTCCGCGTATCCACCAGGTCAGCCCGAGCAATCCGGCAGTGAACAGCAAAAATACCAGGTTCGGCA             >  SRR4302533.1876213/1‑94 (MQ=60)

                                                                                    GGGTTCGGCTGGCGCTTTTTGTTCGCGTCCGCGTATCCACCAGGTCAGCCCGAGCAATCCGGCAGTGAACAGCAAAAATACCAGGTTCGGCATT           <  SRR4302533.2569545/94‑1 (MQ=60)

                                                                                    GGGTTCGGCTGGCGCTTTTTGTTCGCGTCCGCGTATCCACCAGGTCAGCCCGAGCAATCCGGCAGTGAACAGCAAAAATACCAGGTTCGGCATT           <  SRR4302533.426369/94‑1 (MQ=60)

                                                                                    GGGTTCGGCTGGCGCTTTTTGTTCGCGTCCGCGTATCCACCAGGTCAGCCCGAGCAATCCGGCAGTGAACAGCAAAAATACCAGGTTCGGCATT           >  SRR4302533.315500/1‑94 (MQ=60)

                                                                                     GGTTCGGCTGGCGCTTTTTGTTCGCGTCCGCGTATCCACCAGGTCAGCCCGAGCAATCCGGCAGTGAACAGCAAAAATACCAGGTTCGGCATTC          >  SRR4302533.5629530/1‑94 (MQ=60)

                                                                                        TCGGCTGGCGCTTTTTGTTCGCGTCCGCGTATCCACCAGGTCAGCCCGAGCAATCCGGCAGTGAACAGCAAAAATACCAGGTTCGGCATTCCAG       <  SRR4302533.5576417/94‑1 (MQ=60)

                                                                                         CGGCTGGCGCTTTTTGTTCGCGTCCGCGTATCCACCAGGTCAGCCCGAGCAATCCGGCAGTGAACAGCAAAAATACCAGGTTCGGCATTCCAGG      <  SRR4302533.5832857/94‑1 (MQ=60)

                                                                                         CGGCTGGCGCTTTTTGTTCGCGTCCGCGTATCCACCAGGTCAGCCCGAGCAATCCGGCAGTGAACAGCAAAAATACCAGGTTCGGCATTCCAGG      <  SRR4302533.5803084/94‑1 (MQ=60)

                                                                                         CGGCTGGCGCTTTTTGTTCGCGTCCGCGTATCCACCAGGTCAGCCCGAGCAATCCGGCAGTGAACAGCAAAAATACCAGGTTCGGCATTCCAGG      <  SRR4302533.3949503/94‑1 (MQ=60)

                                                                                          GGCTGGCGCTTTTTGTTCGCGTCCGCGTATCCACCAGGTCAGCCCGAGCAATCCGGCAGTGAACAGCAAAAATACCAGGTTCGGCATTCCAGGC     <  SRR4302533.5470262/94‑1 (MQ=60)

                                                                                          GGCTGGCGCTTTTTGTTCGCGTCCGCGTATCCACCAGGTCAGCCCGAGCAATCCGGCAGTGAACAGCAAAAATACCAGGTTCGGCATTCCAGGC     >  SRR4302533.5267842/1‑94 (MQ=60)

                                                                                             TGGCGCTTTTTGTTCGCGTCCGCGTATCCACCAGGTCAGCCCGAGCAATCCGGCAGTGAACAGCAAAAATACCAGGTTCGGCATTCCAGGCACC  >  SRR4302533.5231002/1‑94 (MQ=60)

TTCCATTCCCAGAGAATCTTCCAGTTGTACATCGTTCCACGTCGCTTCGACAACGGTATTATTCTCTGCCATTTTTACCGGTTTGGGTTCGGCAGGCGCTTTTTGTTCGCGTCCGCGTATCCACCAGGCCAGCCCGAGCAATCCGGCAGTGAACAGCAAAAATACCAGGTTCGGCATTCCAGGCACC  >  NC_000913/1961977‑1962163

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`