Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1624	250.6	12620048	98.0%	12367647	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,044,033	(C)_5→6	coding (1149/7152 nt)	b1978 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,044,028	1	.	C	93.3%	632.4 / 36.3	208	coding (1144/7152 nt)	b1978	hypothetical protein
Reads supporting (aligned to +/- strand): ref base . (1/13); new base C (119/75); total (120/88)
Fisher's exact test for biased strand distribution p-value = 7.23e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 234 total aligned reads displayed.

TCTATGAACAGTATTATGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑A‑CCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGTTTTGCCGTCGATTTTACCT  >  NC_000913/2043936‑2044115

  tATGAACAGTATTATGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑A‑cc                                                                                       >  2:3381348/1‑93 (MQ=255)

  tATGAACAGTATTATGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑A‑cc                                                                                       >  2:73525/1‑93 (MQ=255)

  tATGAACAGTATTATGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑A‑cc                                                                                       >  1:321443/1‑93 (MQ=255)

  tATGAACAGTATTATGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑A‑cc                                                                                       >  2:1618521/1‑93 (MQ=255)

  tATGAACAGTATTATGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑A‑cc                                                                                       <  2:5666233/93‑1 (MQ=255)

  tATGAACAGTATTATGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑A‑cc                                                                                       <  1:1874187/93‑1 (MQ=255)

  tATGAACAGTATTATGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑A‑cc                                                                                       >  2:4399159/1‑93 (MQ=255)

       aCAGTATTATGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑A‑cccccct                                                                                  >  1:5739769/1‑91 (MQ=255)

        cAGTATTATGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑A‑cccccctt                                                                                 >  2:1837779/1‑90 (MQ=255)

         aGTATTATGGCGATGAAGTGGCTCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑A‑ccccccttc                                                                                <  1:4352865/93‑5 (MQ=255)

           tattatGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑ACCCCCCTTccc                                                                               >  1:1537072/1‑93 (MQ=255)

           tattatGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑ACCCCCCTTccc                                                                               >  1:5628214/1‑93 (MQ=255)

           tataatGGCGATGAAGTGGCCCTGATAGATACAGACGTACGGCACAGTTATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑ACCCCCCTTccc                                                                               <  1:6307652/93‑1 (MQ=255)

            attatGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCg                                                                              >  1:5199015/1‑93 (MQ=255)

             ttatGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGc                                                                             <  2:4009419/93‑1 (MQ=255)

              tatGGGGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCt                                                                            <  2:5239179/93‑1 (MQ=255)

                 ggCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCtgat                                                                         >  2:3474098/1‑93 (MQ=255)

                  gCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCtgatg                                                                        >  2:3030546/1‑93 (MQ=255)

                    gATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGAc                                                                      >  2:326516/1‑93 (MQ=255)

                      tGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCt                                                                    >  1:4682969/1‑93 (MQ=255)

                      tGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCt                                                                    >  1:2937784/1‑93 (MQ=255)

                      tGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCt                                                                    >  1:4565450/1‑93 (MQ=255)

                       gAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCtt                                                                   >  1:291274/1‑93 (MQ=255)

                       gAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCtt                                                                   >  1:3703404/1‑93 (MQ=255)

                        aaGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTc                                                                  >  2:1405685/1‑93 (MQ=255)

                        aaGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTc                                                                  <  2:3918924/93‑1 (MQ=255)

                        aaGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTc                                                                  <  1:6132321/93‑1 (MQ=255)

                        aaGTGGCCCTGTTCGATAAAGACGATCAGTAGAGTAATCCTCATGCCATAACCGCTGGACTTAACTATT‑C‑CCCCCTTCCCGCTGATGACCTTc                                                                  <  2:5795987/93‑1 (MQ=255)

                         aGTGGCACTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAATCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCa                                                                 <  1:3379995/93‑1 (MQ=255)

                          gTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAg                                                                >  2:3456308/1‑93 (MQ=255)

                            ggCCCTGTTCGCTAAAGCAGATTGGAAAAGTAATCTTCATGCCATAACCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAgcg                                                              <  1:2036867/93‑1 (MQ=255)

                            ggCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAgcg                                                              >  1:6194294/1‑93 (MQ=255)

                             gCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAgcgc                                                             <  1:1041526/93‑1 (MQ=255)

                               ccTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCgg                                                           >  1:2338017/1‑93 (MQ=255)

                                cTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGGa                                                          <  2:1261600/93‑1 (MQ=255)

                                cTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGGa                                                          >  2:316154/1‑93 (MQ=255)

                                 tGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGGAg                                                         >  2:2739706/1‑93 (MQ=255)

                                 tGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGGAg                                                         <  2:5035277/93‑1 (MQ=255)

                                 tGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGGAg                                                         >  1:2522265/1‑93 (MQ=255)

                                  gTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGGAGc                                                        >  1:3281438/1‑93 (MQ=255)

                                   ttCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCa                                                       >  2:4876/1‑93 (MQ=255)

                                   ttCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCa                                                       >  1:5312380/1‑93 (MQ=255)

                                    tCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCaa                                                      <  1:4790092/93‑1 (MQ=255)

                                    tCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCaa                                                      >  2:4788224/1‑93 (MQ=255)

                                    tCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCaa                                                      <  1:1741101/93‑1 (MQ=255)

                                    tCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGAGCGGAGCaa                                                      >  1:5916138/1‑93 (MQ=255)

                                     cGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAAc                                                     <  1:3710623/93‑1 (MQ=255)

                                       aTAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGc                                                   <  1:4780524/93‑1 (MQ=255)

                                        tAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGcc                                                  >  1:264967/1‑93 (MQ=255)

                                          aaGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAg                                                >  1:2094745/1‑93 (MQ=255)

                                             ccactCGCCAAAGTACTCCTCATCCCATACCCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGt                                             <  2:556018/89‑1 (MQ=255)

                                              cGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTa                                            <  1:1457348/93‑1 (MQ=255)

                                              cGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTa                                            <  1:1285373/93‑1 (MQ=255)

                                               gATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTaa                                           <  1:1373921/93‑1 (MQ=255)

                                                 tCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑TCC‑CCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAAc                                         <  1:1146155/93‑1 (MQ=255)

                                                  cGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACa                                        <  2:2047263/93‑1 (MQ=255)

                                                   ggCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAg                                       >  1:3979160/1‑93 (MQ=255)

                                                   ggCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAg                                       >  1:1583497/1‑93 (MQ=255)

                                                   ggCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAg                                       >  2:2301038/1‑93 (MQ=255)

                                                    gCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAgg                                      <  1:2968798/93‑1 (MQ=255)

                                                    gCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAgg                                      <  1:5297514/93‑1 (MQ=255)

                                                      aaaGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑TCC‑CCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGc                                    <  2:3430912/93‑1 (MQ=255)

                                                      aaaGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGc                                    >  1:6238733/1‑93 (MQ=255)

                                                       aaGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCg                                   <  1:5438434/93‑1 (MQ=255)

                                                           aaTCCTCATGCCATAACCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGaaaa                               >  2:2139404/1‑93 (MQ=255)

                                                            aTCCTCATGCCATAACCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAAt                              >  1:4967066/1‑93 (MQ=255)

                                                             tCCTCATGCCATAACCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGATCATCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATg                             >  2:6289994/1‑93 (MQ=255)

                                                               cTCATGCCATAACCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGCAAATGac                           >  1:4487139/1‑93 (MQ=255)

                                                                tCATGCCATAACCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGaca                          >  1:4068620/1‑93 (MQ=255)

                                                                 cATGCCATAACCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGacac                         <  1:2255764/93‑1 (MQ=255)

                                                                  aTGCCATAACCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACAcc                        >  1:4895190/1‑93 (MQ=255)

                                                                    gCCATAACCGCTGGACTTAACTA‑TCC‑CCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAACGACACCCg                      <  2:6152851/93‑1 (MQ=255)

                                                                    gCCATAACCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCg                      >  2:403998/1‑93 (MQ=255)

                                                                    gCCATAACCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCg                      >  1:3802409/1‑93 (MQ=255)

                                                                     ccATAACCGCTGGACTTAACTATT‑C‑CCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGt                     <  1:2052572/93‑1 (MQ=255)

                                                                      cATAACCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCgat                    >  2:1160753/1‑91 (MQ=255)

                                                                      cATAACCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGtt                    <  1:850496/93‑1 (MQ=255)

                                                                        tAACCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGtttt                  <  1:985865/93‑1 (MQ=255)

                                                                        tAACCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGtttt                  >  2:1509008/1‑93 (MQ=255)

                                                                         aaCCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGTTTTg                 <  2:2641462/93‑1 (MQ=255)

                                                                          aCCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGTTTTGc                >  1:2873047/1‑93 (MQ=255)

                                                                           ccGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGTTTTGcc               <  2:813526/93‑1 (MQ=255)

                                                                           ccGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGTTTTGcc               >  2:681571/1‑93 (MQ=255)

                                                                           ccGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGTTTTGcc               >  2:4378624/1‑93 (MQ=255)

                                                                           ccGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGTTTTGcc               <  1:126012/93‑1 (MQ=255)

                                                                           ccGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGTTTTGcc               >  1:1496983/1‑93 (MQ=255)

                                                                            cGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGTTTTGCCg              >  1:2957284/1‑93 (MQ=255)

                                                                               tGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGTTTTAccgccg           >  1:4178072/1‑93 (MQ=255)

                                                                                ggACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGTTTTGCCGTCGa          >  2:4651771/1‑93 (MQ=255)

                                                                                ggACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGTTTTGCCGTCGa          >  2:4247935/1‑93 (MQ=255)

                                                                                ggACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGTTTTGCCGTCGa          >  1:1220519/1‑93 (MQ=255)

                                                                                 gACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGTTTTGCCGTCGAt         >  2:1332834/1‑93 (MQ=255)

                                                                                  aCTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGTTTTGCCGTCGAtt        >  2:5151229/1‑93 (MQ=255)

                                                                                      accTA‑T‑ACCCCCCTTCCCGCTGATGGCCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACCCCCGTTTTGCCGTCGATTTTac    <  2:311799/91‑1 (MQ=255)

                                                                                      aaCTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGTTTTGCCGTCGATTTTac    <  2:2896059/93‑1 (MQ=255)

                                                                                      aaCTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGTTTTGCCGTCGATTTTac    <  2:5886048/93‑1 (MQ=255)

                                                                                      aaCTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGTTTTGCCGTCGATTTTac    >  2:601249/1‑93 (MQ=255)

                                                                                       aCTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGTTTTGCCGTCGATTTTacc   <  1:5680446/93‑1 (MQ=255)

                                                                                       aCTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGTTTTGCCGTCGATTTTacc   >  2:3768635/1‑93 (MQ=255)

                                                                                        cTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGTTTTGCCGTCGATTTTacct  <  2:5821732/93‑1 (MQ=255)

TCTATGAACAGTATTATGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑A‑CCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGTTTTGCCGTCGATTTTACCT  >  NC_000913/2043936‑2044115

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

TGAACAGTATTATGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑A‑CCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGTTTTGCCGTCGATTTTACCTGGCAACCTGGC  >  NC_000913/2043940‑2044126

TGAACAGTATTATGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑A‑CCCCC                                                                                               >  SRR4302533.2964249/1‑94 (MQ=60)

 GAACAGTATTATGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑A‑CCCCCC                                                                                              >  SRR4302533.6180879/1‑94 (MQ=60)

  AACAGTATTATGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑A‑CCCCCCT                                                                                             >  SRR4302533.5774406/1‑94 (MQ=60)

  AACAGTATTATGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑A‑CCCCCCT                                                                                             <  SRR4302533.2733386/94‑1 (MQ=60)

   ACAGTATTATGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑A‑CCCCCctt                                                                                            >  SRR4302533.4173539/1‑91 (MQ=60)

     AGTATTATGGCGATGAAGTGGCTCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑ACCCCCCTTCC                                                                                           <  SRR4302533.4379134/94‑1 (MQ=60)

      GTATTATGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑ACCCCCCTTCCC                                                                                          >  SRR4302533.1546306/1‑94 (MQ=60)

      GTATTATGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑ACCCCCCTTCCC                                                                                          >  SRR4302533.5662244/1‑94 (MQ=60)

       TATTATGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCG                                                                                         >  SRR4302533.5230363/1‑94 (MQ=60)

       TATAATGGCGATGAAGTGGCCCTGATAGATACAGACGTACGGCACAGTTATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCG                                                                                         <  SRR4302533.6345476/94‑1 (MQ=50)

        ATTATGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑A‑CCCCCcttcacgc                                                                                       >  SRR4302533.3805045/1‑86 (MQ=60)

           ATGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGA                                                                                     <  SRR4302533.5488205/94‑1 (MQ=60)

           ATGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGA                                                                                     >  SRR4302533.4817033/1‑94 (MQ=60)

            TGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGAT                                                                                    >  SRR4302533.4638287/1‑94 (MQ=60)

             GGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATG                                                                                   <  SRR4302533.2993737/94‑1 (MQ=60)

             GGCGATGAAGGGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATG                                                                                   >  SRR4302533.3703303/1‑94 (MQ=60)

              GCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGA                                                                                  <  SRR4302533.1559519/94‑1 (MQ=60)

              GCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGA                                                                                  <  SRR4302533.1487480/94‑1 (MQ=60)

               GGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGAC                                                                                 <  SRR4302533.3853445/94‑1 (MQ=60)

                 ATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCT                                                                               >  SRR4302533.488585/1‑94 (MQ=60)

                 ATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCT                                                                               <  SRR4302533.3731313/94‑1 (MQ=60)

                 ATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCT                                                                               >  SRR4302533.2955738/1‑94 (MQ=60)

                 ATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCT                                                                               >  SRR4302533.4711342/1‑94 (MQ=60)

                 ATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCT                                                                               >  SRR4302533.4593130/1‑94 (MQ=60)

                  TGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTT                                                                              >  SRR4302533.3726150/1‑94 (MQ=60)

                  TGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTT                                                                              >  SRR4302533.293047/1‑94 (MQ=60)

                    AAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCA                                                                            <  SRR4302533.6169269/94‑1 (MQ=60)

                     AGTTGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAG                                                                           <  SRR4302533.943463/94‑1 (MQ=60)

                     AGTGGCACTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAATCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAG                                                                           <  SRR4302533.3400859/94‑1 (MQ=60)

                       TGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCG                                                                         >  SRR4302533.6231462/1‑94 (MQ=60)

                        GGCCCTGTTCGCTAAAGCAGATTGGAAAAGTAATCTTCATGCCATAACCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGC                                                                        <  SRR4302533.2049427/94‑1 (MQ=60)

                         GCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCG                                                                       <  SRR4302533.1047650/94‑1 (MQ=60)

                         GCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCG                                                                       >  SRR4302533.2061600/1‑94 (MQ=60)

                          CCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGG                                                                      >  SRR4302533.2352489/1‑94 (MQ=60)

                           CCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGGA                                                                     >  SRR4302533.1590832/1‑94 (MQ=60)

                           CCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGGA                                                                     <  SRR4302533.1168925/94‑1 (MQ=60)

                            CTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGGAG                                                                    >  SRR4302533.2537806/1‑94 (MQ=60)

                             TGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGGAGC                                                                   >  SRR4302533.3301697/1‑94 (MQ=60)

                             TGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGGAGC                                                                   >  SRR4302533.4244947/1‑94 (MQ=60)

                              GTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCA                                                                  >  SRR4302533.5344217/1‑94 (MQ=60)

                               TTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGAGCGGAGCAA                                                                 >  SRR4302533.5951894/1‑94 (MQ=60)

                                TCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAAC                                                                <  SRR4302533.1751657/94‑1 (MQ=60)

                                TCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAAC                                                                <  SRR4302533.4819110/94‑1 (MQ=60)

                                 CGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACG                                                               <  SRR4302533.3733411/94‑1 (MQ=60)

                                 CGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACG                                                               >  SRR4302533.2936701/1‑94 (MQ=60)

                                  GATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGC                                                              >  SRR4302533.4034240/1‑94 (MQ=60)

                                  GATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGC                                                              >  SRR4302533.5963422/1‑94 (MQ=60)

                                  GATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGAGCGGAGCAACGC                                                              >  SRR4302533.2572488/1‑94 (MQ=60)

                                   ATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCC                                                             >  SRR4302533.266579/1‑94 (MQ=60)

                                   ATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCC                                                             <  SRR4302533.3113836/94‑1 (MQ=60)

                                   ATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCC                                                             <  SRR4302533.1385576/94‑1 (MQ=60)

                                   ATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCC                                                             <  SRR4302533.4809486/94‑1 (MQ=60)

                                    TAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCA                                                            >  SRR4302533.1774892/1‑94 (MQ=60)

                                     AAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAG                                                           >  SRR4302533.2107643/1‑94 (MQ=60)

                                     AAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACATTCAGCGCGGAGCAACGCCAG                                                           >  SRR4302533.2219684/1‑94 (MQ=60)

                                      AAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGG                                                          <  SRR4302533.2086709/94‑1 (MQ=60)

                                       AGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGG                                                         >  SRR4302533.1148944/1‑94 (MQ=60)

                                          CGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAA                                                      <  SRR4302533.1466136/94‑1 (MQ=60)

                                          CGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAA                                                      <  SRR4302533.1293104/94‑1 (MQ=60)

                                           GATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAA                                                     <  SRR4302533.1382186/94‑1 (MQ=60)

                                             TCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑TCC‑CCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACA                                                   <  SRR4302533.1152995/94‑1 (MQ=60)

                                              CGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAG                                                  >  SRR4302533.1593040/1‑94 (MQ=60)

                                              CGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAG                                                  <  SRR4302533.4492294/94‑1 (MQ=60)

                                              CGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAG                                                  >  SRR4302533.4003385/1‑94 (MQ=60)

                                              CGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAG                                                  >  SRR4302533.134630/1‑94 (MQ=60)

                                                GCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGG                                                <  SRR4302533.2986950/94‑1 (MQ=60)

                                                GCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGG                                                <  SRR4302533.5329244/94‑1 (MQ=60)

                                                 CAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGC                                               >  SRR4302533.6276094/1‑94 (MQ=60)

                                                  AAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCG                                              <  SRR4302533.4803176/94‑1 (MQ=60)

                                                   AAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGA                                             <  SRR4302533.5471189/94‑1 (MQ=60)

                                                    AGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑TGC‑CCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAA                                            <  SRR4302533.4583149/94‑1 (MQ=60)

                                                     GTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAA                                           >  SRR4302533.735095/1‑94 (MQ=60)

                                                     GTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAA                                           >  SRR4302533.4615835/1‑94 (MQ=60)

                                                      TAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAA                                          >  SRR4302533.2435238/1‑94 (MQ=60)

                                                       AATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAAT                                         >  SRR4302533.4997177/1‑94 (MQ=60)

                                                       AATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAAT                                         >  SRR4302533.5284996/1‑94 (MQ=60)

                                                        ATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATG                                        <  SRR4302533.1982502/94‑1 (MQ=60)

                                                         TCCTCATGCCATAACCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAGAATGA                                       >  SRR4302533.2980935/1‑94 (MQ=60)

                                                         TCCTCATGCCATAACCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGA                                       <  SRR4302533.1522438/94‑1 (MQ=60)

                                                          CCTCATGCCATAACCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGCAAATGAC                                      >  SRR4302533.4514305/1‑94 (MQ=60)

                                                          CCTCATGCCATAACCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGAC                                      >  SRR4302533.3836462/1‑94 (MQ=60)

                                                          CCTCATGCCATAACCGCTAGACTTAGCTA‑T‑ACCCCCCTGCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGAC                                      <  SRR4302533.3336047/94‑1 (MQ=60)

                                                           CTCATGCCATAACCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACA                                     >  SRR4302533.4093388/1‑94 (MQ=60)

                                                            TCATGCCATAACCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACAC                                    <  SRR4302533.5511111/94‑1 (MQ=60)

                                                             CATGCCATAACCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACC                                   >  SRR4302533.4924856/1‑94 (MQ=60)

                                                             CATGCCATAACCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACC                                   <  SRR4302533.2269717/94‑1 (MQ=60)

                                                               TGCCATAACCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCG                                 >  SRR4302533.3825677/1‑94 (MQ=60)

                                                               TGCCATAACCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCG                                 >  SRR4302533.230349/1‑94 (MQ=60)

                                                                GCCATAACCGCTGGACTTAACTATT‑C‑CCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGT                                <  SRR4302533.1414188/94‑1 (MQ=60)

                                                                 CCATAACCGCTGGACTTAACTATT‑C‑CCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGTT                               <  SRR4302533.2065237/94‑1 (MQ=60)

                                                                  CATAACCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGTTT                              <  SRR4302533.855475/94‑1 (MQ=60)

                                                                  CATAACCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGTTT                              <  SRR4302533.6280901/94‑1 (MQ=60)

                                                                    TAACCGCTGGACTTAACTA‑TCC‑CCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGTTTTG                            <  SRR4302533.1267752/94‑1 (MQ=60)

                                                                    TAACCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGTTTTG                            <  SRR4302533.991635/94‑1 (MQ=60)

                                                                     AACCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGTTTTGC                           >  SRR4302533.5862855/1‑94 (MQ=60)

                                                                     AACCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGTTTTGC                           >  SRR4302533.126513/1‑94 (MQ=60)

                                                                     AACCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGTTTTGC                           >  SRR4302533.2937519/1‑94 (MQ=60)

                                                                     AACCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGTTTTGC                           >  SRR4302533.2890725/1‑94 (MQ=60)

                                                                      ACCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGTTTTGCC                          >  SRR4302533.1505999/1‑94 (MQ=60)

                                                                       CCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGTTTTGCCG                         >  SRR4302533.1594515/1‑94 (MQ=60)

                                                                       CCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGTTTTGCCG                         >  SRR4302533.258720/1‑94 (MQ=60)

                                                                       CCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGTTTTGCCG                         <  SRR4302533.126801/94‑1 (MQ=60)

                                                                       CCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGTTTTGCCG                         >  SRR4302533.4952714/1‑94 (MQ=60)

                                                                       CCGCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGTTTTGCCG                         >  SRR4302533.2975358/1‑94 (MQ=60)

                                                                         GCTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGTTTTGCCGTC                       >  SRR4302533.5337667/1‑94 (MQ=60)

                                                                          CTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGTTTTGCCGTCG                      >  SRR4302533.5681830/1‑94 (MQ=60)

                                                                          CTGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGTTTTACCGCCG                      >  SRR4302533.4203384/1‑94 (MQ=60)

                                                                           TGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGTTTTGCCGTCGA                     >  SRR4302533.1227845/1‑94 (MQ=60)

                                                                           TGGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGTTTTGCCGTCGA                     >  SRR4302533.571777/1‑94 (MQ=60)

                                                                            GGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGTTTTGCCGTCGAT                    >  SRR4302533.1886802/1‑94 (MQ=60)

                                                                            GGACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATAACACCCGTTTTGCCGTCGAT                    >  SRR4302533.5025436/1‑94 (MQ=60)

                                                                             GACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGTTTTGCCGTCGATT                   >  SRR4302533.591606/1‑94 (MQ=60)

                                                                              ACTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGTTTTGCCGTCGATTT                  <  SRR4302533.4854587/94‑1 (MQ=60)

                                                                               CTTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGTTTTGCCGTCGATTTT                 >  SRR4302533.761124/1‑94 (MQ=60)

                                                                                TTAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGTTTTGCCGTCGATTTTA                <  SRR4302533.3605190/94‑1 (MQ=60)

                                                                                 TAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGTTTTGCCGTCGATTTTAC               <  SRR4302533.2269551/94‑1 (MQ=60)

                                                                                 TAACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGTTTTGCCGTCGATTTTAC               <  SRR4302533.4373425/94‑1 (MQ=60)

                                                                                   ACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGTTTTGCCGTCGATTTTACCT             <  SRR4302533.6245976/94‑1 (MQ=60)

                                                                                   ACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGTTTTGCCGTCGATTTTACCT             <  SRR4302533.3281461/94‑1 (MQ=60)

                                                                                   ACTA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGTTTTGCCGTCGATTTTACCT             <  SRR4302533.5714756/94‑1 (MQ=60)

                                                                                     TA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGTTTTGCCGTCGATTTTACCTGG           <  SRR4302533.2841824/94‑1 (MQ=60)

                                                                                     TA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGTTTTGCCGTCGATTTTACCTGG           <  SRR4302533.726247/94‑1 (MQ=60)

                                                                                     TA‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGTTTTGCCGTCGATTTTACCTGG           <  SRR4302533.3967803/94‑1 (MQ=60)

                                                                                      A‑T‑ACCCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAAAGCCAGGGTAAACAGGGCGAAAATGACACCAGTTTTGCCGTCGATTTTACCTGGC          >  SRR4302533.5410711/1‑94 (MQ=60)

                                                                                        T‑C‑CCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGTTTTGCCGTCGATTTTACCTGGCAA        <  SRR4302533.4273690/94‑1 (MQ=60)

                                                                                         tacCCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGTTTTGCCGTCGATTTTACCTGGCA         >  SRR4302533.2643196/4‑94 (MQ=60)

                                                                                          C‑CCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGTTTTGCCGTCGATTTTACCTGGCAAC       >  SRR4302533.1288370/1‑94 (MQ=60)

                                                                                          C‑CCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGTTTTGCCGTCGATTTTACCTGGCAAC       >  SRR4302533.1659471/1‑94 (MQ=60)

                                                                                            CCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGTTTTGCCGTCGATTTTACCTGGCAACC      >  SRR4302533.3735480/1‑94 (MQ=60)

                                                                                             CCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGTTTTGCCGTCGATTTTACCTGGCAACCT     >  SRR4302533.2411590/1‑94 (MQ=60)

                                                                                               CCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGTTTTGCCGTCGATTTTACCTGGCAACCTGG   <  SRR4302533.4516555/94‑1 (MQ=60)

                                                                                                CTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGTTTTGCCGTCGATTTTACCTGGCAACCTGGC  >  SRR4302533.4515394/1‑94 (MQ=60)

TGAACAGTATTATGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTA‑T‑A‑CCCCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGTTTTGCCGTCGATTTTACCTGGCAACCTGGC  >  NC_000913/2043940‑2044126

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 8 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`