Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,078,049 A→G F189L (TTC→CTC)  yeeA ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,078,0490AG100.0% 586.8 / NA 180F189L (TTC→CTC) yeeAhypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (85/95);  total (85/95)

Only 100 of 183 total aligned reads displayed.

GCGCAATCAGTCCACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAATGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCCGTTGTGGCCAGATACCGG  >  NC_000913/2077959‑2078138
                                                                                          |                                                                                         
gcgcAATCAGTCCACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTg                                                                                         <  1:5492595/93‑1 (MQ=255)
  gcAATCAGTCCACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCa                                                                                       <  2:549894/93‑1 (MQ=255)
  gcAATCAGTCCACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCa                                                                                       <  2:5137758/93‑1 (MQ=255)
  gcAATCAGTCCACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCa                                                                                       >  2:4890565/1‑93 (MQ=255)
  gcAATCAGTCCACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCa                                                                                       <  2:4555206/93‑1 (MQ=255)
  gcAATCAGTCCACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGGGAGAGTGCa                                                                                       >  2:2118879/1‑93 (MQ=255)
  gcAATCAGTCCACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGACTAAGTTCGGTGAGAGTGCa                                                                                       <  1:2095728/93‑1 (MQ=255)
    aaTCAGTCCACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGa                                                                                     >  1:2822618/1‑93 (MQ=255)
    aaTCAGTCCACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGa                                                                                     <  2:1661828/93‑1 (MQ=255)
      tCAGTCCACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGAtt                                                                                   >  1:6012279/1‑93 (MQ=255)
         gTCCACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGAt                                                                                <  2:2388471/93‑1 (MQ=255)
          tCCACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATa                                                                               <  1:2508908/93‑1 (MQ=255)
          tCCACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATa                                                                               <  1:41216/93‑1 (MQ=255)
           ccgcGCATTTTCACGTCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTTCAGATTGATAg                                                                              <  2:1524521/90‑1 (MQ=255)
           ccACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAg                                                                              <  1:1984402/93‑1 (MQ=255)
            cACGCATTTTCGCGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGa                                                                             <  2:1272307/93‑1 (MQ=255)
             aCGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGAc                                                                            >  1:5021052/1‑93 (MQ=255)
              cGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGAcc                                                                           >  2:4810545/1‑93 (MQ=255)
                cATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCg                                                                         <  1:4007377/93‑1 (MQ=255)
                  ttttCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGAt                                                                       >  1:5216130/1‑93 (MQ=255)
                  ttttCACGGCATCGGTCAGGAGGTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGAt                                                                       <  2:2084562/93‑1 (MQ=255)
                    ttCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATta                                                                     <  1:3420669/93‑1 (MQ=255)
                    ttCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATta                                                                     <  2:4024604/93‑1 (MQ=255)
                    ttCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATta                                                                     <  1:570788/93‑1 (MQ=255)
                       aCGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATAc                                                                  <  2:2507210/93‑1 (MQ=255)
                        cGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACt                                                                 >  2:4337081/1‑93 (MQ=255)
                         ggCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGAGCCGATTGATAGACCCGATTATACTc                                                                >  1:782401/1‑93 (MQ=255)
                         ggCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGATGAGAGTGCAGATTGATAGACCCGATTATACTc                                                                <  1:2729421/93‑1 (MQ=255)
                          gCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCg                                                               >  1:4483291/1‑93 (MQ=255)
                          gCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCg                                                               <  2:842450/93‑1 (MQ=255)
                           cATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCgg                                                              <  1:3228987/93‑1 (MQ=255)
                           cATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCgg                                                              >  2:356117/1‑93 (MQ=255)
                            aTCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGt                                                             >  2:5442254/1‑93 (MQ=255)
                            aTCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGt                                                             <  1:1435305/93‑1 (MQ=255)
                              cGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCa                                                           <  2:1178043/93‑1 (MQ=255)
                              cGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCAAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCa                                                           <  1:1767282/93‑1 (MQ=255)
                                gTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAgc                                                         >  2:4469371/1‑92 (MQ=255)
                                gTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTACACTCGGTCAGa                                                         >  2:2591946/1‑93 (MQ=255)
                                 tCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGAc                                                        >  2:963883/1‑93 (MQ=255)
                                 tCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGAc                                                        <  1:2180329/93‑1 (MQ=255)
                                    ggAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACttt                                                     >  2:5654902/1‑93 (MQ=255)
                                    ggAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACttt                                                     >  2:4617491/1‑93 (MQ=255)
                                     gAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACtttt                                                    <  2:6246052/93‑1 (MQ=255)
                                     gAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACtttt                                                    <  1:3544768/93‑1 (MQ=255)
                                     gAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACtttt                                                    >  2:5959694/1‑93 (MQ=255)
                                     gAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACtttt                                                    >  1:753204/1‑93 (MQ=255)
                                     gAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACtttt                                                    <  1:1935061/93‑1 (MQ=255)
                                      aGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTc                                                   <  1:3020424/93‑1 (MQ=255)
                                      aGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTc                                                   <  1:1711027/93‑1 (MQ=255)
                                         ttttGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGcc                                                <  1:5752360/93‑1 (MQ=255)
                                             gTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGtt                                            >  2:3453986/1‑93 (MQ=255)
                                                gATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGaa                                         <  1:5984994/93‑1 (MQ=255)
                                                 aTGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAAt                                        <  2:1365757/93‑1 (MQ=255)
                                                   ggCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATgc                                      >  1:255940/1‑93 (MQ=255)
                                                   ggCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATgc                                      <  2:5959782/93‑1 (MQ=255)
                                                      tttCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCTAGTTGAATGCGcc                                   >  1:5341266/1‑93 (MQ=255)
                                                      tttCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGcc                                   >  1:2634490/1‑93 (MQ=255)
                                                       ttCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCa                                  <  2:5389758/93‑1 (MQ=255)
                                                        tCCAGACGTGGGCGTTCTAGTAAGTTCGGTGAGAGTGCAGATTGATAGCCCCGATTATCCTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAg                                 <  2:4735116/93‑1 (MQ=255)
                                                        tCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAg                                 >  1:2012188/1‑93 (MQ=255)
                                                         ccAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGt                                >  1:3075648/1‑93 (MQ=255)
                                                         ccAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGt                                <  1:5693340/93‑1 (MQ=255)
                                                          cAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTg                               >  2:3855282/1‑93 (MQ=255)
                                                           aGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTgg                              >  1:1207902/1‑93 (MQ=255)
                                                           aGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTgg                              >  1:5265973/1‑93 (MQ=255)
                                                           aGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTgg                              >  2:1585894/1‑93 (MQ=255)
                                                           aGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTgg                              <  1:345727/93‑1 (MQ=255)
                                                            gACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTggt                             >  1:1400029/1‑92 (MQ=255)
                                                            gACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGa                             >  2:1461281/1‑93 (MQ=255)
                                                              cGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATg                           <  1:1352331/93‑1 (MQ=255)
                                                              cGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATg                           >  1:2965725/1‑93 (MQ=255)
                                                                tGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGaa                         <  2:2811692/93‑1 (MQ=255)
                                                                 gggCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAAc                        <  2:2835677/93‑1 (MQ=255)
                                                                 gggCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAAc                        <  2:3888463/93‑1 (MQ=255)
                                                                   gCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGc                      >  2:205133/1‑93 (MQ=255)
                                                                     gTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGccc                    <  2:769552/93‑1 (MQ=255)
                                                                     gTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGccc                    >  1:6207767/1‑93 (MQ=255)
                                                                     gTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGccc                    >  2:4664132/1‑93 (MQ=255)
                                                                      ttCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCCg                   >  2:5894143/1‑93 (MQ=255)
                                                                      ttCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCCg                   <  1:4416886/93‑1 (MQ=255)
                                                                       tCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCCGt                  <  1:1993885/93‑1 (MQ=255)
                                                                       tCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCCGt                  <  2:1419756/93‑1 (MQ=255)
                                                                       tCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCCGt                  >  2:475610/1‑93 (MQ=255)
                                                                        cGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCCGtt                 >  1:3897432/1‑93 (MQ=255)
                                                                          aGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCCGTtgt               <  2:2995830/93‑1 (MQ=255)
                                                                          aGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCCGTtgt               <  2:368167/93‑1 (MQ=255)
                                                                          aGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCCGTtgt               >  2:3352886/1‑93 (MQ=255)
                                                                           gTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCCGTtgtg              >  1:4940437/1‑93 (MQ=255)
                                                                           gTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCCGTtgtg              >  1:5065406/1‑93 (MQ=255)
                                                                              aGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCCGTTGTGGcc           <  1:3575781/93‑1 (MQ=255)
                                                                                 tCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCCGTTGTGGCCAGa        <  2:5020991/93‑1 (MQ=255)
                                                                                 tCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCCGTTGTGGCCAGa        <  1:4007886/93‑1 (MQ=255)
                                                                                  cGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCCGTTGTGGCCAGAt       <  2:5298473/93‑1 (MQ=255)
                                                                                   ggTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCCGTTGTGGCCAGATa      >  1:2418954/1‑93 (MQ=255)
                                                                                   ggTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGCGGATGAACGCCCGTTGTGGCCAGCTa      >  2:4285132/1‑93 (MQ=255)
                                                                                    gTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCCGTTGTGGCCAGATAc     <  2:2705534/93‑1 (MQ=255)
                                                                                     tGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCCGTTGTGGCCAGATAcc    <  1:1606541/93‑1 (MQ=255)
                                                                                     tGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCCGTTGTGGCCAGATAcc    <  1:3714576/93‑1 (MQ=255)
                                                                                     tGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCCGTTGTGGCCAGATAcc    >  2:3753111/1‑93 (MQ=255)
                                                                                     tGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCCGTTGTGGCCAGATAcc    >  2:3715589/1‑93 (MQ=255)
                                                                                          |                                                                                         
GCGCAATCAGTCCACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAATGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCCGTTGTGGCCAGATACCGG  >  NC_000913/2077959‑2078138

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CGGGCGCAATCAGTCCACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAATGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCCGTTGTGGCCAGATACCGGTAAA  >  NC_000913/2077956‑2078142
                                                                                             |                                                                                             
CGGGCGCAATCAGTCCACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAG                                                                                               <  SRR4302533.2070171/94‑1 (MQ=60)
  GGCGCAATCAGTCCACGCATTTTCACTGCAACGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGGGGGCGTTCGAGTAAGTTCGGTGAGAGTG                                                                                             <  SRR4302533.1433015/94‑1 (MQ=60)
  GGCGCAATCAGTCCACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTG                                                                                             <  SRR4302533.476017/94‑1 (MQ=60)
   GCGCAATCAGTCCACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGC                                                                                            <  SRR4302533.5525724/94‑1 (MQ=60)
    CGCAATCAGTCCACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCA                                                                                           >  SRR4302533.2967637/1‑94 (MQ=60)
    CGCAATCAGTCCACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCA                                                                                           <  SRR4302533.3911799/94‑1 (MQ=60)
     GCAATCAGTCCACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGACTAAGTTCGGTGAGAGTGCAG                                                                                          <  SRR4302533.2108632/94‑1 (MQ=60)
      CAATCAGTCCACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGA                                                                                         >  SRR4302533.2839988/1‑94 (MQ=60)
      CAATCAGTCCACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGA                                                                                         >  SRR4302533.2643374/1‑94 (MQ=60)
        ATCAGTCCACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATT                                                                                       >  SRR4302533.6048704/1‑94 (MQ=60)
         TCAGTCCACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTG                                                                                      >  SRR4302533.5493536/1‑94 (MQ=60)
          CAGTCCACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGA                                                                                     >  SRR4302533.3334278/1‑94 (MQ=60)
            GTCCACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATA                                                                                   >  SRR4302533.4445411/1‑94 (MQ=60)
             TCCACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAG                                                                                  <  SRR4302533.41498/94‑1 (MQ=60)
             TCCACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAG                                                                                  <  SRR4302533.2524366/94‑1 (MQ=60)
              CCACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGA                                                                                 <  SRR4302533.1620008/94‑1 (MQ=60)
              CCACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGA                                                                                 <  SRR4302533.1996542/94‑1 (MQ=60)
               CACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGAC                                                                                >  SRR4302533.5051461/1‑94 (MQ=60)
               CACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGCGCAGATTGATAGAC                                                                                >  SRR4302533.3004182/1‑94 (MQ=60)
                ACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACC                                                                               <  SRR4302533.5712762/94‑1 (MQ=60)
                   CATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGA                                                                            <  SRR4302533.4031775/94‑1 (MQ=60)
                    ATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGAT                                                                           >  SRR4302533.5247534/1‑94 (MQ=60)
                     TTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATT                                                                          <  SRR4302533.2238709/94‑1 (MQ=60)
                       TTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTAT                                                                        <  SRR4302533.3441805/94‑1 (MQ=60)
                       TTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTAT                                                                        <  SRR4302533.574158/94‑1 (MQ=60)
                        TCNCNGNANCGNTNAGGAGNTTTTGTAGATNGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATA                                                                       <  SRR4302533.152746/94‑1 (MQ=60)
                           CGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGAGCCGATTGATAGACCCGATTATACTC                                                                    >  SRR4302533.787004/1‑94 (MQ=60)
                            GGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCG                                                                   >  SRR4302533.4510439/1‑94 (MQ=60)
                            GGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGATGAGAGTGCAGATTGATAGACCCGATTATACTCG                                                                   <  SRR4302533.2746162/94‑1 (MQ=60)
                             GCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGG                                                                  >  SRR4302533.1978228/1‑94 (MQ=60)
                              CATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGT                                                                 <  SRR4302533.3248895/94‑1 (MQ=60)
                               ATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTC                                                                >  SRR4302533.3204891/1‑94 (MQ=60)
                               ATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTC                                                                <  SRR4302533.1443969/94‑1 (MQ=60)
                                 CGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAG                                                              <  SRR4302533.6300874/94‑1 (MQ=60)
                                 CGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCAAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAG                                                              <  SRR4302533.1778042/94‑1 (MQ=60)
                                  GGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGA                                                             <  SRR4302533.4120640/94‑1 (MQ=60)
                                  GGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGA                                                             >  SRR4302533.5251199/1‑94 (MQ=60)
                                    TCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACT                                                           <  SRR4302533.2193771/94‑1 (MQ=60)
                                       GGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTT                                                        <  SRR4302533.5743798/94‑1 (MQ=60)
                                       GGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTT                                                        >  SRR4302533.757641/1‑94 (MQ=60)
                                        GAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTC                                                       <  SRR4302533.2012595/94‑1 (MQ=60)
                                        GAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTC                                                       <  SRR4302533.6050625/94‑1 (MQ=60)
                                        GAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTC                                                       <  SRR4302533.3566616/94‑1 (MQ=60)
                                        GAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTC                                                       <  SRR4302533.1946865/94‑1 (MQ=60)
                                         AGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCG                                                      <  SRR4302533.5133338/94‑1 (MQ=60)
                                         AGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCG                                                      <  SRR4302533.3038911/94‑1 (MQ=60)
                                         AGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCG                                                      <  SRR4302533.1721396/94‑1 (MQ=60)
                                            TTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCA                                                   <  SRR4302533.5787067/94‑1 (MQ=60)
                                                   GATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAAT                                            <  SRR4302533.30661/94‑1 (MQ=60)
                                                   GATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAAT                                            <  SRR4302533.6021241/94‑1 (MQ=60)
                                                     TGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGC                                          >  SRR4302533.257503/1‑94 (MQ=60)
                                                        CTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCTAGTTGAATGCGCC                                       >  SRR4302533.5373298/1‑94 (MQ=60)
                                                        CTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCC                                       >  SRR4302533.2650679/1‑94 (MQ=60)
                                                        CTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCC                                       >  SRR4302533.809753/1‑94 (MQ=60)
                                                         TTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCA                                      <  SRR4302533.3725369/94‑1 (MQ=60)
                                                         TTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCA                                      <  SRR4302533.1419386/94‑1 (MQ=60)
                                                         TTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCA                                      >  SRR4302533.5323321/1‑94 (MQ=60)
                                                          TTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAG                                     >  SRR4302533.2024584/1‑94 (MQ=60)
                                                           TCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGT                                    <  SRR4302533.3586270/94‑1 (MQ=60)
                                                           TCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGT                                    >  SRR4302533.3094524/1‑94 (MQ=60)
                                                            CCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTG                                   <  SRR4302533.5727721/94‑1 (MQ=60)
                                                             CAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGG                                  >  SRR4302533.1215155/1‑94 (MQ=60)
                                                             CAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGG                                  >  SRR4302533.5297573/1‑94 (MQ=60)
                                                              AGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGT                                 >  SRR4302533.1408467/1‑94 (MQ=60)
                                                              AGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGA                                 <  SRR4302533.347839/94‑1 (MQ=60)
                                                              AGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGA                                 <  SRR4302533.2727150/94‑1 (MQ=60)
                                                              AGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGA                                 >  SRR4302533.2534788/1‑94 (MQ=60)
                                                                ACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATG                               >  SRR4302533.2983852/1‑94 (MQ=60)
                                                                 CGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGA                              <  SRR4302533.1360464/94‑1 (MQ=60)
                                                                 CGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGA                              <  SRR4302533.4256296/94‑1 (MQ=60)
                                                                   TGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAAC                            <  SRR4302533.4556318/94‑1 (MQ=60)
                                                                   TGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAAC                            <  SRR4302533.3934522/94‑1 (MQ=60)
                                                                    GGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACG                           >  SRR4302533.4974426/1‑94 (MQ=60)
                                                                       CGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCC                        >  SRR4302533.6244998/1‑94 (MQ=60)
                                                                         TTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCCGT                      <  SRR4302533.4443592/94‑1 (MQ=60)
                                                                          TCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCCGTT                     <  SRR4302533.2006115/94‑1 (MQ=60)
                                                                          TCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCCGTT                     >  SRR4302533.3921214/1‑94 (MQ=60)
                                                                           CGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCCGTTG                    >  SRR4302533.1378786/1‑94 (MQ=60)
                                                                             AGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCCGTTGTG                  >  SRR4302533.5096001/1‑94 (MQ=60)
                                                                             AGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCCGTTGTG                  >  SRR4302533.4970345/1‑94 (MQ=60)
                                                                                AAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATGCTCGGTCAGACTTGTCGCCAGTTGAATGGGCCAGTGGATGAAGGCCCGTTGcgccc               >  SRR4302533.1507617/1‑89 (MQ=60)
                                                                                AAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCCGTTGTGGCC               >  SRR4302533.2002698/1‑94 (MQ=60)
                                                                                 AGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCCGTTGTGGCCA              <  SRR4302533.3597821/94‑1 (MQ=60)
                                                                                 AGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCCGTTGTGGCCA              >  SRR4302533.6042242/1‑94 (MQ=60)
                                                                                 AGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCCGTTGTGGCCA              >  SRR4302533.3561495/1‑94 (MQ=60)
                                                                                    TCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCCGTTGTGGCCAGAT           <  SRR4302533.4032291/94‑1 (MQ=60)
                                                                                     CGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCCGTTGTGGCCAGATA          >  SRR4302533.2433896/1‑94 (MQ=60)
                                                                                       GTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCCGTTGTGGCCAGATACC        >  SRR4302533.2040782/1‑94 (MQ=60)
                                                                                       GTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCCGTTGTGGCCAGATACC        <  SRR4302533.156523/94‑1 (MQ=60)
                                                                                        TGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCCGTTGTGGCCAGATACCG       <  SRR4302533.1874450/94‑1 (MQ=60)
                                                                                        TGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCCGTTGTGGCCAGATACCG       <  SRR4302533.1616292/94‑1 (MQ=60)
                                                                                        TGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCCGTTGTGGCCAGATACCG       <  SRR4302533.3737382/94‑1 (MQ=60)
                                                                                         GAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCCGTTGTGGCCAGATACCGG      <  SRR4302533.2479463/94‑1 (MQ=60)
                                                                                          AGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCCGTTGTGGCCAGATACCGGT     <  SRR4302533.5868198/94‑1 (MQ=60)
                                                                                             GTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCCGTTGTGGCCAGATACCGGTAAA  >  SRR4302533.5890253/1‑94 (MQ=60)
                                                                                             GTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCCGTTGTGGCCAGATACCGGTAAA  >  SRR4302533.5390194/1‑94 (MQ=60)
                                                                                             |                                                                                             
CGGGCGCAATCAGTCCACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAATGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCCGTTGTGGCCAGATACCGGTAAA  >  NC_000913/2077956‑2078142

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑