Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,137,410 A→G D496G (GAT→GGT)  b2063 → putative transport protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,137,4100AG100.0% 669.1 / NA 208D496G (GAT→GGT) b2063putative transport protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (113/95);  total (113/95)

Only 100 of 225 total aligned reads displayed.

ATGAAAAACGTGAATATCACACCATTGCCGGGCTGTTGATGGAGTATTTGCAGCGTATTCCAAAACCTGGCGAAGAAGTTCAGGTGGGGGATTATTTGCTTAAAACGTTGCAGGTAGAAAGCCATCGCGTGCAGAAGGTGCAGATTATACCGCTGCGTAAGGATGGCGAGATGGAGTACG  >  NC_000913/2137320‑2137499
                                                                                          |                                                                                         
 tGAAAAACGTGAATATCACACCATTGCCGGGCTGTTGATGGAGTATTTGCAGCGTATTCCAAAACCTGGCGAAGAAGTTCAGGTGGGGGgttt                                                                                        >  2:2060035/1‑92 (MQ=255)
 tGAAAAACGTGAATATCACACCATTGCCGGGCTGTTGATGGAGTATTTGCAGCGTATTCCAAAACCTGGCGAAGAAGTTCAGGTGGGGGgtta                                                                                        >  1:1018763/1‑93 (MQ=255)
   aaaaaCGTGAATATCACACCATTGCCGGGCTGTTGATGGAGTATTTGCAGCGTATTCCAAAACCTGGCGAAGAAGTTCAGGTGGGGGgttatt                                                                                      <  1:3569247/93‑1 (MQ=255)
   aaaaaCGTGAATATCACACCATTGCCGGGCTGTTGATGGAGTATTTGCAGCGTATTCCAAAACCTGGAGAAGAAGTTCAGGTGGGGGgttatt                                                                                      >  2:4230125/1‑93 (MQ=255)
    aaaaCGTGAATATCACACCATTGCCGGGCTGTTGATGGAGTATTTGCAGCGTATTCCAAAACCTGGCGAAGAAGTTCAGGTGGGGGGTTAttt                                                                                     >  1:1500712/1‑93 (MQ=255)
    aaaaCGTGAATATCACACCATTGCCGGGCTGTTGATGGAGTATTTGCAGCGTATTCCAAAACCTGGCGAAGAAGTTCAGGTGGGGGGTTAttt                                                                                     >  2:256258/1‑93 (MQ=255)
    aaaaCGTGAATATCACACCATTGCCGGGCTGTTGATGGAGTATTTGCAGCGTATTCCAAAACCTGGCGAAGAAGTTCAGGTGGGGGGTTAttt                                                                                     >  2:5816467/1‑93 (MQ=255)
      aaCGTGAATATCACACCATTGCCGGGCTGTTGATGGAGTATTTGCAGCGTATTCCAAAACCTGGCGAAGAAGTTCAGGTGGGGGGTTATTTGc                                                                                   <  1:1143923/93‑1 (MQ=255)
         gTGAATATCACACCATTGCCGGGCTGTTGATGGAGTATTTGCAGCGTATTCCAAAACCTGGCGAAGAAGTTCAGGTGGGGGGTTATTTGCTTa                                                                                >  2:884472/1‑93 (MQ=255)
         gTGAATATCACACCATTGCCGGGCTGTTGATGGAGTATTTGCAGCGTATTCCAAAACCTGGCGAAGAAGTTCAGGCGGGGGGGTATTTGCTTa                                                                                >  2:629007/1‑93 (MQ=255)
          tGAATATCACACCATTGCCGGGCTGTTGATGGAGTATTTGCAGCGTATTCCAAAACCTGGCGAAGAAGTTCAGGTGGGGGGTTATTTGCTTaa                                                                               <  2:2742610/93‑1 (MQ=255)
          tGAATATCACACCATTGCCGGGCTGTTGATGGAGTATTTGCAGCGTATTCCAAAACCTGGCGAAGAAGTTCAGGTGGGGGGTTATTTGCTTaa                                                                               >  1:1944304/1‑93 (MQ=255)
          tGAATATCACACCATTGCCGGGCTGTTGATGGAGTATTTGCAGCGTATTCCAAAACCTGGCGAAGAAGTTCAGGTGGGGGGTTATTTGCTTaa                                                                               <  1:544987/93‑1 (MQ=255)
           gAATATCACACCATTGCCGGGCTGTTGATGGAGTATTTGCAGCGTATTCCAAAACCTGGCGAAGAAGTTCAGGTGGGGGGTTATTTGCTTaaa                                                                              >  1:239408/1‑93 (MQ=255)
            aaTATCACACCATTGCCGGGCTGTTGATGGAGTATTTGCAGCGTATTCCAAAACCTGGCGAAGAAGTTCAGGTGGGGGGTTATTTGCTTaaaa                                                                             >  2:1435871/1‑93 (MQ=255)
            aaTATCACACCATTGCCGGGCTGTTGATGGAGTATTTGCAGCGTATTCCAAAACCTGGCGAAGAAGTTCAGGTGGGGGGTTATTTGCTTaaaa                                                                             <  1:3284452/93‑1 (MQ=255)
                tCACACCATTGCCGGGCTGTTGATGGAGTATTTGCAGCGTATTCCAAAACCTGGCGAAGAAGTTCAGGTGGGGGGTTATTTGCTTAAAACGtt                                                                         >  1:4901835/1‑93 (MQ=255)
                 ctctccATTGCCGGGCTGTTGATGGAGTATTTGCAGCGTATTCCAAAACCTGGCGAAGAAGTTCAGGTGGGGGGTTATTTGCTTAAAACGTTg                                                                        <  1:4678319/89‑1 (MQ=255)
                  acacCATTGCCGGGCTGTTGATGGAGTATTTGCAGCGTATTCCAAAACCTGGCGAAGAAGTTCAGGTGGGGGGTTATTTGCTTAAAACGTTGc                                                                       <  2:4816655/93‑1 (MQ=255)
                  acacCATTGCCGGGCTGTTGATGGAGTATTTGCAGCGTATTCCAAAACCTGGCGAAGAAGTTCAGGTGGGGGGTTATTTGCTTAAAACGTTGc                                                                       >  1:508644/1‑93 (MQ=255)
                    acCATTGCCGGGCTGTTGATGGAGTATTTGCAGCGTATTCCAAAACCTGGCGAAGAAGTTCAGGTGGGGGGTTATTTGCTTAAAACGTTGCAg                                                                     <  1:2451175/93‑1 (MQ=255)
                      cATTGCCGGGCTGTTGATGGAGTATTTGCAGCGTATTCCAAAACCTGGCGAAGAAGTTCAGGTGGGGGGTTATTTGCTTAAAACGTTGCAGGt                                                                   >  1:1403657/1‑93 (MQ=255)
                       aTTGCCGGGCTGTTGATGGAGTATTTGCAGCGTATTCCAAAACCTGGCGAAGAAGTTCAGGTGGGGGGTTATTTGCTTAAAACGTTGCAGGTa                                                                  >  1:315005/1‑93 (MQ=255)
                        ttGCCGGGCTGTTGATGGAGTATTTGCAGCGTATTCCAAAACCTGGCGAAGAAGTTCAGGTGGGGGGTTATTTGCTTAAAACGTTGCAGGTAg                                                                 <  1:5809450/93‑1 (MQ=255)
                          gCCGGGCTGTTGATGGAGTATTTGCAGCGTATTCCAAAACCTGGCGAAGAAGTTCAGGTGGGGGGTTATTTGCTTAAAACGTTGCAGGTAGaa                                                               <  2:4595237/93‑1 (MQ=255)
                          gCCGGGCTGTTGATGGAGTATTTGCAGCGTATTCCAAAACCTGGCGAAGAAGTTCAGGTGGGGGGTTATTTGCTTAAAACGTTGCAGGTAGaa                                                               >  1:2181581/1‑93 (MQ=255)
                               gCTGTTGATGGAGTATTTGCAGCGTATTCCAAAACCTGGCGAAGAAGTTCAGGTGGGGGGTTATTTGCTTAAAACGTTGCAGGTAGAAAGCCa                                                          <  2:2958378/93‑1 (MQ=255)
                                cTGTTGATGGAGTATTTGCAGCGTATTCCAAAACCTGGCGAAGAAGTTCAGGAGGGGGGTTATTTGCTTAAAACGTTGCAGGTAGAAAGCCAt                                                         <  2:5134289/93‑1 (MQ=255)
                                 tGTTGATGGAGTATTTGCAGCGTATTCCAAAACCTGGCGAAGAAGTTCAGGTGGGGGGTTATTTGCTTAAAACGTTGCAGGTAGAAAGCCATc                                                        >  2:1373940/1‑93 (MQ=255)
                                  gTTGATGGAGTATTTGCAGCGTATTCCAAAACCTGGCGAAGAAGTTCAGGTGGGGGGTTATTTGCTTAAAACGTTGCAGGTAGAAAGCCATcg                                                       >  2:5866153/1‑93 (MQ=255)
                                  gTTGATGGAGTATTTGCAGCGTATTCCAAAACCTGGCGAAGAAGTTCAGGTGGGGGGTTATTTGCTTAAAACGTTGCAGGTAGAAAGCCATcg                                                       >  2:1010499/1‑93 (MQ=255)
                                   ttGATGGAGTATTTGCAGCGTATTCCAAAACCTGGCGAAGAAGTTCAGGTGGGGGGTTATTTGCTTAAAACGTTGCAGGTAGAAAGCCATcgc                                                      <  2:851740/93‑1 (MQ=255)
                                   ttGATGGAGTATTTGCAGCGTATTCCAAAACCTGGCGAAGAAGTTCAGGTGGGGGGTTATTTGCTTAAAACGTTGCAGGTAGAAAGCCATcgc                                                      <  1:3732564/93‑1 (MQ=255)
                                   ttGATGGAGTATTTGCAGCGTATTCCAAAACCTGGCGAAGAAGTTCAGGTGGGGGGTTATTTGCTTAAAACGTTGCAGGTAGAAAGCCATcgc                                                      <  1:432927/93‑1 (MQ=255)
                                     gATGGAGTATTTGCAGCGTATTCCAAAACCTGGCGAAGAAGTTCAGGTGGGGGGTTATTTGCTTAAAACGTTGCAGGTAGAAAGCCATCGCGt                                                    <  1:2882950/93‑1 (MQ=255)
                                     gATGGAGTATTTGCAGCGTATTCCAAAACCTGGCGAAGAAGTTCAGGTGGGGGGTTATTTGCTTAAAACGTTGCAGGTAGAAAGCCATCGCGt                                                    <  1:3093739/93‑1 (MQ=255)
                                     gATGGAGTATTTGCAGCGTATTCCAAAACCTGGCGAAGAAGTTCAGGTGGGGGGTTATTTGCTTAAAACGTTGCAGGTAGAAAGCCATCGCGt                                                    <  2:1349115/93‑1 (MQ=255)
                                     gATGGAGTATTTGCAGCGTATTCCAAAACCTGGCGAAGAAGTTCAGGTGGGGGGTTATTTGCTTAAAACGTTGCAGGTAGAAAGCCATCGCGt                                                    >  2:5987410/1‑93 (MQ=255)
                                      aTGGAGTATTTGCAGCGTATTCCAAATCCTGGCGAAGAAGTTCAGGTGGGGGGTTATTTGCTTAAAACGTTGCAGGTAGAAAGCCATCGCGTg                                                   <  1:603247/93‑1 (MQ=255)
                                        ggAGTATTTGCAGCGTATTCCAAAACCTGGCGAAGAAGTTCAGGTGGGGGGTTATTTGCTTAAAACGTTGCAGGTAGAAAGCCATCGCGTGCa                                                 >  2:3256306/1‑93 (MQ=255)
                                           gTATTTGCAGCGTATTCCAAAACCTGGCGAAGAAGTTCAGGTGGGGGGTTATTTGCTTAAAACGTTGCAGGTAGAAAGCCATCGCGTGCAGaa                                              <  1:2647442/93‑1 (MQ=255)
                                            tATTTGCAGCGTATTCCAAATCCTGGCGAAGAAGTTCAGGTGGGGGGTTATTTGCTTAAAACGTTGCAGGTAGAAAGCCATCGCGTGCAGAAg                                             <  2:3128639/93‑1 (MQ=255)
                                             aTTTGCAGCGTATTCCAAAACCTGGCGAAGAAGTTCAGGTGGGGGGTTATTTGCTTAAAACGTTGCAGGTAGAAAGCCATCGCGTGCAGAAgg                                            >  2:2341589/1‑93 (MQ=255)
                                              tttGCAGCGTATTCCAAAACCTGGCGAAGAAGTTCAGGTGGGGGGTTATTTGCTTAAAACGTTGCAGGTAGAAAGCCATCGCGTGCAGAAGGt                                           <  1:5950277/93‑1 (MQ=255)
                                               ttGCAGCGTATTCCAAAACCTGGCGAAGAAGTTCAGGTGGGGGGTTATTTGCTTAAAACGTTGCAGGTAGAAAGCCATCGCGTGCAGAAGGTg                                          >  2:5529929/1‑93 (MQ=255)
                                                 gCAGCGTATTCCAAAACCTGGCGAAGAAGTTCAGGTGGGGGGTTATTTGCTTAAAACGTTGCAGGTAGAAAGCCATCGCGTGCAGAAGGTGCa                                        <  2:5681194/93‑1 (MQ=255)
                                                   aGCGTATTCCAAAACCTGGCGAAGAAGTTCAGGTGGGGGGTTATTTGCTTAAAACGTTGCAGGTAGAAAGCCATCGCGTGCAGAAGGTGCAGa                                      <  2:5067083/93‑1 (MQ=255)
                                                   aGCGTATTCCAAAACCTGGCGAAGAAGTTCAGGTGGGGGGTTATTTGCTTAAAACGTTGCAGGTAGAAAGCCATCGCGTGCAGAAGGTGCAGa                                      <  2:1546441/93‑1 (MQ=255)
                                                    gCGTATTCCAAAACCTGGCGGAGAAGTTCAGGTGGGGGGTTATTTGCTTAAAACGTTGCAGGTAGAAAGCCATCGCGTGCAGAAGGTGCAGAt                                     >  2:4888017/1‑93 (MQ=255)
                                                    gCGTATTCCAAAACCTGGCGAAGAAGTTCAGGTGGGGGGTTATTTGCTTAAAACGTTGCAGGTAGAAAGCCATCGCGTGCAGAAGGTGCAGAt                                     >  1:4957671/1‑93 (MQ=255)
                                                    gCGTATTCCAAAACCTGGCGAAGAAGTTCAGGTGGGGGGTTATTTGCTTAAAACGTTGCAGGTAGAAAGCCATCGCGTGCAGAAGGTGCAGAt                                     >  1:3412417/1‑93 (MQ=255)
                                                    gCGTATTCCAAAACCTGGCGAAGAAGTTCAGGTGGGGGGTTATTTGCTTAAAACGTTGCAGGTAGAAAGCCATCGCGTGCAGAAGGTGCAGAt                                     >  1:1718807/1‑93 (MQ=255)
                                                     cGTATTCCAAAACCTGGCGAAGAAGTTCAGGTGGGGGGTTATTTGCTTAAAACGTTGCAGGTAGAAAGCCATCGCGTGCAGAAGGTGCAGAtt                                    <  1:4022629/93‑1 (MQ=255)
                                                      gTATTCCAAAACCTGGCGAAGAAGTTCAGGTGGGGGGTTATTTGCTTAAAACGTTGCAGGTAGAAAGCCATCGCGTGCAGAAGGTGCAGATta                                   >  1:2300897/1‑93 (MQ=255)
                                                       tATTCCAAAACCTGGCGAAGAAGTTCAGGTGGGGGGTTATTTGCTTAAAACGTTGCAGGTAGAAAGCCATCGCGTGCAGAAGGTGCAGATtat                                  >  1:4707416/1‑93 (MQ=255)
                                                        aTTCCAAAACCTGGCGAAGAAGTTCAGGTGGGGGGTTATTTGCTTAAAACGTTGCAGGTAGAAAGCCATCGCGTGCAGAAGGTGCAGATtata                                 >  2:1060267/1‑93 (MQ=255)
                                                         ttCCAAAACCTGGCGAAGAAGTTCAGGTGGGGGGTTATTTGCTTAAAACGTTGCAGGTAGAAAGCCATCGCGTGCAGAAGGTGCAGATTATAc                                >  2:4092995/1‑93 (MQ=255)
                                                         ttCCAAAACCTGGCGAAGAAGTTCAGGTGGGGGGTTATTTGCTTAAAACGTTGCAGGTAGAAAGCCATCGCGTGCAGAAGGTGCAGATTATAc                                >  2:1822271/1‑93 (MQ=255)
                                                         ttCCAAAACCTGGCGAAGAAGTTCAGGTGGGGGGTTATTTGCTTAAAACGTTGCAGGTAGAAAGCCATCGCGTGCAGAAGGTGCAGATTATAc                                >  1:5390554/1‑93 (MQ=255)
                                                         ttCCAAAACCTGGCGAAGAAGTTCAGGTGGGGGGTTATTTGCTTAAAACGTTGCAGGTAGAAAGCCATCGCGTGCAGAAGGTGCAGATTATAc                                <  1:1335571/93‑1 (MQ=255)
                                                          tCCAAAACCTGGCGAAGAAGTTCAGGTGGGGGGTTATTTGCTTAAAACGTTGCAGGTAGAAAGCCATCGCGTGCAGAAGGTGCAGATTATAcc                               >  2:1454198/1‑93 (MQ=255)
                                                            cAAAACCTGGCGAAGAAGTTCAGGTGGGGGGTTATTTGCTTAAAACGTTGCAGGTAGAAAGCCATCGCGTGCAGAAGGTGCAGATTATACCGc                             <  2:221341/93‑1 (MQ=255)
                                                            cAAAACCTGGCGAAGAAGTTCAGGTGGGGGGTTATTTGCTTAAAACGTTGCAGGTAGAAAGCCATCGCGTGCAGAAGGTGCAGATTATACCGc                             <  2:2535337/93‑1 (MQ=255)
                                                               aaCCTGGCGAAGAAGTTCAGGTGGGGGGTTATTTGCTTAAAACGTTGCAGGTAGAAAGCCATCGCGTGCAGAAGGTGCAGATTATACCGCTGc                          <  1:1010348/93‑1 (MQ=255)
                                                                  cTGGCGAAGAAGTTCAGGTGGGGGGTTATTTGCTTAAAACGTTGCAGGTAGAAAGCCATCGCGTGCAGAAGGTGCAGATTATACCGCTGCGTa                       >  1:4461610/1‑93 (MQ=255)
                                                                  cTGGCGAAGAAGTTCAGGTGGGGGGTTATTTGCTTAAAACGTTGCAGGTAGAAAGCCATCGCGTGCAGAAGGTGCAGATTATACCGCTGCGTa                       >  1:805364/1‑93 (MQ=255)
                                                                  cTGGCGAAGAAGTTCAGGTGGGGGGTTATTTGCTTAAAACGTTGCAGGTAGAAAGCCATCGCGTGCAGAAGGTGCAGATTATACCGCTGCATa                       >  1:553615/1‑93 (MQ=255)
                                                                   tGGCGAAGAAGTTCAGGTGGGGGGTTATTTGCTTAAAACGTTGCAGGTAGAAAGCCATCGCGTGCAGAAGGTGCAGATTATACCGCTGCGTaa                      >  1:5276225/1‑93 (MQ=255)
                                                                   tGGCGAAGAAGTTCAGGTGGGGGGTTATTTGCTTAAAACGTTGCAGGTAGAAAGCCATCGCGTGCAGAAGGTGCAGATTATACCGCTGCGTaa                      >  2:3599455/1‑93 (MQ=255)
                                                                    ggCGAAGAAGTTCAGGTGGGGGGTTATTTGCTTAAAACGTTGCAGGTAGAAAGCCATCGCGTGCAGAAGGTGCAGATTATACCGCTGCGTAAg                     <  2:1190413/93‑1 (MQ=255)
                                                                    ggCGAAGAAGTTCAGGTGGGGGGTTATTTGCTTAAAACGTTGCAGGTAGAAAGCCATCGCGTGCAGAAGGTGCAGATTATACCGCTGCGTAAg                     >  2:798870/1‑93 (MQ=255)
                                                                      ggaagaaGTTCAGGTGGGGGGTTATTTGCTTAAAACGTTGCAGGTAGAAAGCCATCGCGTGCAGAAGGTGCAGATTATACCGCTGCGTAAGGa                   <  2:4476723/92‑1 (MQ=255)
                                                                       gaagaaGTTCAGGTGGGGGGTTATTTGCTTAAAACGTTGCAGGTAGAAAGCCATCGCGTGCAGAAGGTGCAGATTATACCGCTGCGTAAGGAt                  <  2:289977/93‑1 (MQ=255)
                                                                       gaagaaGTTCAGGTGGGGGGTTATTTGCTTAAAACGTTGCAGGTAGAAAGCCATCGCGTGCAGAAGGTGCAGATTATACCGCTGCGTAAGGAt                  >  2:3807325/1‑93 (MQ=255)
                                                                       gaagaaGTTCAGGTGGGGGGTTATTTGCTTAAAACGTTGCAGGTAGAAAGCCATCGCGTGCAGAAGGTGCAGATTATACCGCTGCGTAAGGAt                  <  1:5562149/93‑1 (MQ=255)
                                                                       gaagaaGTTCAGGTGGGGGGTTATTTGCTTAAAACGTTGCAGGTAGAAAGCCATCGCGTGCAGAAGGTGCAGATTATACCGCTGCGTAAGGAt                  <  1:1174351/93‑1 (MQ=255)
                                                                        aagaagTTCAGGTGGGGGGTTATTTGCTTAAAACGTTGCAGGTAGAAAGCCATCGCGTGCAGAAGGTGCAGATTATACCGCTGCGTAAGGCTg                 >  1:5867456/1‑93 (MQ=255)
                                                                        aagaagTTCAGGTGGGGGGTTATTTGCTTAAAACGTTGCAGGTAGAAAGCCATCGCGTGCAGAAGGTGCAGATTATACCGCTGCGTAAGGATg                 >  2:3490/1‑93 (MQ=255)
                                                                        aagaagTTCAGGTGGGGGGTTATTTGCTTAAAACGTTGCAGGTAGAAAGCCATCGCGTGCAGAAGGTGCAGATTATACCGCTGCGTAAGGATg                 >  2:268832/1‑93 (MQ=255)
                                                                         agaagTTCAGGTGGGGGGTTATTTGCTTAAAACGTTGCAGGTAGAAAGCCATCGCGTGCAGCAGGTGCAGATTATACCGCTGCGTAAGGATgg                >  2:979716/1‑93 (MQ=255)
                                                                         agaagTTCAGGTGGGGGGTTATTTGCTTAAAACGTTGCAGGTAGAAAGCCATCGCGTGCAGAAGGTGCAGATTATACCGCTGCGTAAGGATgg                >  1:4483749/1‑93 (MQ=255)
                                                                         agaagTTCAGGTGGGGGGTTATTTGCTTAAAACGTTGCAGGTAGAAAGCCATCGCGTGCAGAAGGTGCAGATTATACCGCTGCGTAAGGATgg                >  1:6233732/1‑93 (MQ=255)
                                                                         agaagTTCAGGTGGGGGGTTATTTGCTTAAAACGTTGCAGGTAGAAAGCCATCGCGTGCAGAAGGTGCAGATTATACCGCTGCGTAAGGATgg                <  1:1584379/93‑1 (MQ=255)
                                                                          gaagTTCAGGTGGGGGGTTATTTGCTTAAAACGTTGCAGGTAGAAAGCCATCGCGTGCAGAAGGTGCAGATTATACCGCTGCGTAAGGATGGc               >  2:3092675/1‑93 (MQ=255)
                                                                          gaagTTCAGGTGGGGGGTTATTTGCTTAAAACGTTGCAGGTAGAAAGCCATCGCGTGCAGAAGGTGCAGATTATACCGCTGCGTAAGGATGGc               >  2:5735542/1‑93 (MQ=255)
                                                                           aagTTCAGGTGGGGGGTTATTTGCTTAAAACGTTGCAGGTAGAAAGCCATCGCGTGCAGAAGGTGCAGATTATACCGCTGCGTAAGGATGGCg              >  2:561861/1‑93 (MQ=255)
                                                                           aagTTCAGGTGGGGGGTTATTTGCTTAAAACGTTGCAGGTAGAAAGCCATCGCGTGCAGAAGGTGCAGATTATACCGCTGCGTAAGGATGGCg              >  2:1666639/1‑93 (MQ=255)
                                                                           aagTTCAGGTGGGGGGTTATTTGCTTAAAACGTTGCAGGTAGAAAGCCATCGCGTGCAGAAGGTGCAGATTATACCGCTGCGTAAGGATGGCg              >  1:3842590/1‑93 (MQ=255)
                                                                             gTTCAGGTGGGGGGTTATTTGCTTAAAACGTTGCAGGTAGAAAGCCATCGCGTGCAGAAGGTGCAGATTATACCGCTGCGTAAGGATGGCgag            <  2:4647942/93‑1 (MQ=255)
                                                                              ttCAGGTGGGGGGTTATTTGCTTAAAACGTTGCAGGTAGAAAGCCATCGCGTGCAGAAGGTGCAGATTATACCGCTGCGTAAGGATGGCgaga           <  1:1893413/93‑1 (MQ=255)
                                                                                cAGGTGGGGGGTTATTTGCTTAACGCGTTGCAGGTAGAATGCCATCGCGTGCAGAAGGTGCAGATTATACCGCTCCGTAAGGAGGGCGAGATg         <  2:2302688/93‑1 (MQ=255)
                                                                                cAGGTGGGGGGTTATTTGCTTAAAACGTTGCAGGTAGAAAGCCATCGCGTGCAGAAGGTGCAGATTATACCGCTGCGTAAGGATGGCGAGATg         <  2:4215189/93‑1 (MQ=255)
                                                                                 aGGTGGGGGGTTATTTGCTTAAAACGTTGCAGGTAGAAAGCCATCGCGTGCAGAAGGTGCAGATTATACCGCTGCGTAAGGATGGCGAGATgg        >  1:6022169/1‑93 (MQ=255)
                                                                                    tGGGGGGTTATTTGCTTAAAACGTTGCAGGTAGAAAGCCATCGCGTGCAGAAGGTGCAGATTATACCGCTGCGTAAGGATGGCGAGATGGAGt     <  1:4401817/93‑1 (MQ=255)
                                                                                     ggggggTTATTTGCTTAAAACGTTGCAGGTAGAAAGCCATCGCGTGCAGAAGGTGCAGATTATACCGCTGCGTAAGGATGGCGAGATGGAGTa    <  1:821142/93‑1 (MQ=255)
                                                                                     ggggggTTATTTGCTTAAAACGTTGCAGGTAGAAAGCCATCGCGTGCAGAAGGTGCAGATTATACCGCTGCGTAAGGATGGCGAGATGGAGTa    >  1:2084343/1‑93 (MQ=255)
                                                                                     ggggggTTATTTGCTTAAAACGTTGCAGGTAGAAAGCCATCGCGTGCAGAAGGTGCAGATTATACCGCTGCGTAAGGATGGCGAGATGGAGTa    >  1:4554651/1‑93 (MQ=255)
                                                                                      ggggTTTATTTGATTAAAACGTTGCAGGTAGAAAGCCATCGCGTGCAGAAGGTGCAGATGATACCGCTGCGTAAGGATGGCGAGATGGAGTAc   <  2:4291542/93‑1 (MQ=255)
                                                                                       ggggTTATTTGCTTAAAACGTTGCAGGTAGAAAGCCATCGCGTGCAGAAGGTGCAGATTATACCGCTGCGTAAGGATGGCGAGATGGAGTACg  >  1:2852768/1‑93 (MQ=255)
                                                                                       ggggTTATTTGCTTAAAACGTTGCAGGTAGAAAGCCATCGCGTGCAGAAGGTGCAGATTATACCGCTGCGTAAGGATGGCGAGATGGAGTACg  >  2:6113122/1‑93 (MQ=255)
                                                                                          |                                                                                         
ATGAAAAACGTGAATATCACACCATTGCCGGGCTGTTGATGGAGTATTTGCAGCGTATTCCAAAACCTGGCGAAGAAGTTCAGGTGGGGGATTATTTGCTTAAAACGTTGCAGGTAGAAAGCCATCGCGTGCAGAAGGTGCAGATTATACCGCTGCGTAAGGATGGCGAGATGGAGTACG  >  NC_000913/2137320‑2137499

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TGGATGAAAAACGTGAATATCACACCATTGCCGGGCTGTTGATGGAGTATTTGCAGCGTATTCCAAAACCTGGCGAAGAAGTTCAGGTGGGGGATTATTTGCTTAAAACGTTGCAGGTAGAAAGCCATCGCGTGCAGAAGGTGCAGATTATACCGCTGCGTAAGGATGGCGAGATGGAGTACGAGGT  >  NC_000913/2137317‑2137503
                                                                                             |                                                                                             
TGGATGAAAAACGTGAATATCACACCATTGCCGGGCTGTTGATGGAGTATTTGCAGCGTATTCCAAAACCTGGCGAAGAAGTTCAGGTGGGGGG                                                                                               <  SRR4302533.5518575/94‑1 (MQ=60)
TGGATGAAAAACGTGAATATCACACCATTGCCGGGCTGTTGATGGAGTATTTGCAGCGTATTCCAAAACCTGGCGAAGAAGTTCAGGTGGGGGG                                                                                               >  SRR4302533.5709312/1‑94 (MQ=60)
TGGATGAAAAACGTGAATATCACACCATTGCCGGGCTGTTGATGGAGTATTTGCAGCGTATTCCAAAACCTGGCGAAGAAGTTCAGGTGGGGGG                                                                                               >  SRR4302533.223311/1‑94 (MQ=60)
 GGATGAAAAACGTGAATATCACACCATTGCCGGGCTGTTGATGGAGTATTTGCAGCGTATTCCAAAACCTGGCGAAGAAGTTCAGGTGGGGGGT                                                                                              >  SRR4302533.5463582/1‑94 (MQ=60)
  GATGAAAAACGTGAATATCACACCATTGCCGGGCTGTTGATGGAGTATTTGCAGCGTATTCCAAAACCTGGCGAAGAAGTTCAGGTGGGGGGTT                                                                                             <  SRR4302533.58791/94‑1 (MQ=60)
   ATGAAAAACGTGAATATCACACCATTGCCGGGCTGTTGATGGAGTATTTGCAGCGTATTCCAAAACCTGGCGAAGAAGTTCAGGTGGGGGGTTA                                                                                            >  SRR4302533.1024752/1‑94 (MQ=60)
      AAAAACGTGAATATCACACCATTGCCGGGCTGTTGATGGAGTATTTGCAGCGTATTCCAAAACCTGGCGAAGAAGTTCAGGTGGGGGGTTATTT                                                                                         >  SRR4302533.1509757/1‑94 (MQ=60)
      AAAAACGTGAATATCACACCATTGCCGGGCTGTTGATGGAGTATTTGCAGCGTATTCCAAAACCTGGCGAAGAAGTTCAGGTGGGGGGTTATTT                                                                                         <  SRR4302533.3591246/94‑1 (MQ=60)
         AACGTGAATATCACACCATTGCCGGGCTGTTGATGGAGTATTTGCAGCGTATTCCAAAACCTGGCGAAGAAGTTCAGGTGGGGGGTTATTTGCT                                                                                      <  SRR4302533.1150745/94‑1 (MQ=60)
         AACGTGAATATCACACCATTGCCGGGCTGTTGATGGAGTATTTGCAGCGTATTCCAAAACCTGGCGAAGAAGTTCAGGTGGGGGGTTATTTGCT                                                                                      <  SRR4302533.1245525/94‑1 (MQ=60)
            GTGAATATCACACCATTGCCGGGCTGTTGATGGAGTATTTGCAGCGTATTCCAAAACCTGGCGAAGAAGTTCAGGTGGGGGGTTATTTGCTTAA                                                                                   <  SRR4302533.330904/94‑1 (MQ=60)
            GTGAATATCACACCATTGCCGGGCTGTTGATGGAGTATTTGCAGCGTATTCCAAAACCTGGCGAAGAAGTTCAGGTGGGGGGTTATTTGCTTAA                                                                                   >  SRR4302533.1956163/1‑94 (MQ=60)
             TGAATATCACACCATTGCCGGGCTGTTGATGGAGTATTTGCAGCGTATTCCAAAACCTGGCGAAGAAGTTCAGGTGGGGGGTTATTTGCTTAAA                                                                                  <  SRR4302533.548224/94‑1 (MQ=60)
             TGAATATCACACCATTGCCGGGCTGTTGATGGAGTATTTGCAGCGTATTCCAAAACCTGGCGAAGAAGTTCAGGTGGGGGGTTATTTGCTTAAA                                                                                  >  SRR4302533.240890/1‑94 (MQ=60)
               AATATCACACCATTGCCGGGCTGTTGATGGAGTATTTGCAGCGTATTCCAAAACCTGGCGAAGAAGTTCAGGTGGGGGGTTATTTGCTTAAAAC                                                                                <  SRR4302533.3304725/94‑1 (MQ=60)
               AATATCACACCATTGCCGGGCTGTTGATGGAGTATTTGCAGCGTATTCCAAAACCTGGCGAAGAAGTTCAGGTGGGGGGTTATTTGCTTAAAAC                                                                                <  SRR4302533.820370/94‑1 (MQ=60)
               AATATCACACCATTGCCGGGCTGTTGATGGAGTATTTGCAGCGTATTCCAAAACCTGGCGAAGAAGTTCAGGTGGGGGGTTATTTGCTTAAAAC                                                                                >  SRR4302533.957525/1‑94 (MQ=60)
                ATATCACACCATTGCCGGGCTGTTGATGGAGTATTTGCAGCGTATTCCAAAACCTGGCGAAGAAGTTCAGGTGGGGGGTTATTTGCTTAAAACG                                                                               >  SRR4302533.290077/1‑94 (MQ=60)
                  ATCACACCATTGCCGGGCTGTTGATGGAGTATTTGCAGCGTATTCCAAAACCTGGCGAAGAAGTTCAGGTGGGGGGTTATTTGCTTAAAACGTT                                                                             >  SRR4302533.4931537/1‑94 (MQ=60)
                  ATCACACCATTGCCGGGCTGTTGATGGAGTATTTGCAGCGTATTCCAAAACCTGGCGAAGAAGTTCAGGTGGGGGGTTATTTGCTTAAAACGTT                                                                             <  SRR4302533.1312183/94‑1 (MQ=60)
                   TCACACCATTGCCGGGCTGTTGATGGAGTATTTGCAGCGTATTCCAAAACCTGGCGAAGAAGTTCAGGTGGGGGGTTATTTGCTTAAAACGTTG                                                                            >  SRR4302533.5015943/1‑94 (MQ=60)
                   TCACACCATTGCCGGGCTGTTGATGGAGTATTTGCAGCGTATTCCAAAACCTGGCGAAGAAGTTCAGGTGGGGGGTTATTTGCTTAAAACGTTG                                                                            <  SRR4302533.5936923/94‑1 (MQ=60)
                    ctctCCATTGCCGGGCTGTTGATGGAGTATTTGCAGCGTATTCCAAAACCTGGCGAAGAAGTTCAGGTGGGGGGTTATTTGCTTAAAACGTTGC                                                                           <  SRR4302533.4706671/90‑1 (MQ=60)
                    CACACCATTGCCGGGCTGTTGATGGAGTATTTGCAGCGTATTCCAAATCCTGGCGAAGAAGTTCAGGTGGGGGGTTATTTGCTTAAAACGTTGC                                                                           <  SRR4302533.4114549/94‑1 (MQ=60)
                    CACACCATTGCCGGGCTGTTGATGGAGTATTTGCAGCGTATTCCAAAACCTGGCGAAGAAGTTCAGGTGGGGGGTTATTTGCTTAAAACGTTGC                                                                           >  SRR4302533.511664/1‑94 (MQ=60)
                     ACACCATTGCCGGGCTGTTGATGGAGTATTTGCAGCGTATTCCAAAACCTGGCGAAGAAGTTCAGGTGGGGGGTTATTTGCTTAAAACGTTGCA                                                                          <  SRR4302533.2017730/94‑1 (MQ=60)
                      CACCATTGCCGGGCTGTTGATGGAGTATTTGCAGCGTATTCCAAAACCTGCCGAAGAAGTTCAGGTGGGGGGTTATTTGCTTAAAACGTTGCAG                                                                         <  SRR4302533.4460303/94‑1 (MQ=60)
                       ACCATTGCCGGGCTGTTGATGGAGTATTTGCAGCGTATTCCAAAACCTGGCGAAGAAGTTCAGGTGGGGGGTTATTTGCTTAAAACGTTGCAGG                                                                        <  SRR4302533.2466308/94‑1 (MQ=60)
                        CCATTGCCGGGCTGTTGATGGAGTATTTGCAGCGTATTCCAAAACCTGGCGAAGAAGTTCAGGTGGGGGGTTATTTGCTTAAAACGTTGCAGGT                                                                       >  SRR4302533.1412152/1‑94 (MQ=60)
                         CATTGCCGGGCTGTTGATGGAGTATTTGCAGCGTATTCCAAAACCTGGCGAAGAAGTTCAGGTGGGGGGTTATTTGCTTAAAACGTTGCAGGTA                                                                      >  SRR4302533.316928/1‑94 (MQ=60)
                         CATTGCCGGGCTGTTGATGGAGTATTTGCAGCGTATTCCAAAACCTGGCGAAGAAGTTCAGGTGGGGGGTTATTTGCTTAAAACGTTGCAGGTA                                                                      <  SRR4302533.5999703/94‑1 (MQ=60)
                          ATTGCCGGGCTGTTGATGGAGTATTTGCAGCGTATTCCAAAACCTGGCGAAGAAGTTCAGGTGGGGGGTTATTTGCTTAAAACGTTGCAGGTAG                                                                     <  SRR4302533.606026/94‑1 (MQ=60)
                           TTGCCGGGCTGTTGATGGAGTATTTGCAGCGTATTCCAAAACCTGGCGAAGAAGTTCAGGTGGGGGGTTATTTGCTTAAAACGTTGCAGGTAGA                                                                    <  SRR4302533.5844533/94‑1 (MQ=60)
                            TGCCGGGCTGTTGATGGAGTATTTGCAGCGTATTCCAAAACCTGGCGAAGAAGTTCAGGTGGGGGGTTATTTGCTTAAAACGTTGCAGGTAGAA                                                                   >  SRR4302533.2195031/1‑94 (MQ=60)
                            TGCCGGGCTGTTGATGGAGTATTTGCAGCGTATTCCAAAACCTGGCGAAGAAGTTCAGGTGGGGGGTTATTTGCTTAAAACGTTGCAGGTAGAA                                                                   >  SRR4302533.4570430/1‑94 (MQ=60)
                              CCGGGCTGTTGATGGAGTATTTGCAGCGTATTCCAAAACCTGGCGAAGAAGTTCAGGTGGGGGGTTATTTGCTTAAAACGTTGCAGGTAGAAAG                                                                 <  SRR4302533.5530861/94‑1 (MQ=60)
                                GGGCTGTTGATGGAGTATTTGCAGCGTATTCCAAAACCTGGCGAAGAAGTTCAGGTGGGGGGTTATTTGCTTAAAACGTTGCAGGTAGAAAGCC                                                               <  SRR4302533.345835/94‑1 (MQ=60)
                                GGGCTGTTGATGGAGTATTTGCAGCGTATTCCAAAACCTGGCGAAGAAGTTCAGGTGGGGGGTTATTTGCTTAAAACGTTGCAGGTAGAAAGCC                                                               >  SRR4302533.2630791/1‑94 (MQ=60)
                                 GGCTGTTGATGGAGTATTTGCAGCGTATTCCAAAACCTGGCGAAGAAGTTCAGGTGGGGGGTTATTTGCTTAAAACGTTGCAGGTAGAAAGCCA                                                              >  SRR4302533.1023104/1‑94 (MQ=60)
                                   CTGTTGATGGAGTATTTGCAGCGTATTCCAAAACCTGGCGAAGAAGTTCAGGTGGGGGGTTATTTGCTTAAAACGTTGCAGGTAGAAAGCCATC                                                            >  SRR4302533.5204462/1‑94 (MQ=60)
                                    TGTTGATGGAGTATTTGCAGCGTATTCCAAAACCTGGCGAAGAAGTTCAGGTGGGGGGTTATTTGCTTAAAACGTTGCAGGTAGAAAGCCATCG                                                           >  SRR4302533.3684056/1‑94 (MQ=60)
                                      TTGATGGAGTATTTGCAGCGTATTCCAAAACCTGGCGAAGAAGTTCAGGTGGGGGGTTATTTGCTTAAAACGTTGCAGGTAGAAAGCCATCGCG                                                         <  SRR4302533.3755463/94‑1 (MQ=60)
                                      TTGATGGAGTATTTGCAGCGTATTCCAAAACCTGGCGAAGAAGTTCAGGTGGGGGGTTATTTGCTTAAAACGTTGCAGGTAGAAAGCCATCGCG                                                         <  SRR4302533.435507/94‑1 (MQ=60)
                                       TGATGGAGTATTTGCAGCGTATTCCAAAACCTGGCGAAGAAGTTCAGGTGGGGGGTTATTTGCTTAAAACGTTGCAGGTAGAAAGCCATCGCGT                                                        >  SRR4302533.212959/1‑94 (MQ=60)
                                        GATGGAGTATTTGCAGCGTATTCCAAAACCTGGCGAAGAAGTTCAGGTGGGGGGTTATTTGCTTAAAACGTTGCAGGTAGAAAGCCATCGCGTG                                                       <  SRR4302533.2900684/94‑1 (MQ=60)
                                        GATGGAGTATTTGCAGCGTATTCCAAAACCTGGCGAAGAAGTTCAGGTGGGGGGTTATTTGCTTAAAACGTTGCAGGTAGAAAGCCATCGCGTG                                                       <  SRR4302533.3112761/94‑1 (MQ=60)
                                         ATGGAGTATTTGCAGCGTATTCCAAATCCTGGCGAAGAAGTTCAGGTGGGGGGTTATTTGCTTAAAACGTTGCAGGTAGAAAGCCATCGCGTGC                                                      <  SRR4302533.606792/94‑1 (MQ=60)
                                         ATGGAGTATTTGCAGCGTATTCCAAAACCTGGCGAAGAAGTTCAGGTGGGGGGTTATTTGCTTAAAACGTTGCAGGTAGAAAGCCATCGCGTGC                                                      >  SRR4302533.1389870/1‑94 (MQ=60)
                                         ATGGAGTATTTGCAGCGTATTCCAAAACCTGGCGAAGAAGTTCAGGTGGGGGGTTATTTGCTTAAAACGTTGCAGGTAGAAAGCCATCGCGTGC                                                      >  SRR4302533.5339906/1‑94 (MQ=60)
                                          TGGAGTATTTGCAGCGTATTCCAAAACCTGGCGAAGAAGTTCAGGTGGGGGGTTATTTGCTTAAAACGTTGCAGGTAGAAAGCCATCGCGTGCA                                                     >  SRR4302533.315691/1‑94 (MQ=60)
                                           GGAGTATTTGCAGCGTATTCCAAAACCTGGCGAAGAAGTTCAGGTGGGGGGTTATTTGCTTAAAACGTTGCAGGTAGAAAGCCATCGCGTGCAG                                                    <  SRR4302533.3956773/94‑1 (MQ=60)
                                           GGAGTATTTGCAGCGTATTCCAAAACCTGGCGAAGAAGTTCAGGTGGGGGGTTATTTGCTTAAAACGTTGCAGGTAGAAAGCCATCGCGTGCAG                                                    <  SRR4302533.3851444/94‑1 (MQ=60)
                                              GTATTTGCAGCGTATTCCAAAACCTGGCGAAGAAGTTCAGGTGGGGGGTTATTTGCTTAAAACGTTGCAGGTAGAAAGCCATCGCGTGCAGAAG                                                 <  SRR4302533.2663707/94‑1 (MQ=60)
                                              GTATTTGCAGCGTATTCCAAAACCTGGCGAAGAAGTTCAGGTGGGGGGTTATTTGCTTAAAACGTTGCAGGTAGAAAGCCATCGCGTGCAGAAG                                                 <  SRR4302533.1542487/94‑1 (MQ=60)
                                              GTATTTGCAGCGTATTCCAAAACCTGGCGAAGAAGTTCAGGTGGGGGGTTATTTGCTTAAAACGTTGCAGGTAGAAAGCCATCGCGTGCAGAAG                                                 <  SRR4302533.2322108/94‑1 (MQ=60)
                                              GTATTTGCAGCGTATTCCAAAACCTGGCGAAGAAGTTCAGGTGGGGGGTTATTTGCTTAAAACGTTGCAGGTAGAAAGCCATCGCGTGCAGAAG                                                 <  SRR4302533.1654649/94‑1 (MQ=60)
                                                 TTTGCAGCGTATTCCAAAACCTGGCGAAGAAGTTCAGGTGGGGGGTTATTTGCTTAAAACGTTGCAGGTAGAAAGCCATCGCGTGCAGAAGGTG                                              <  SRR4302533.5986264/94‑1 (MQ=60)
                                                 TTTGCAGCGTATTCCAAAACCTGGCGAAGAAGTTCAGGTGGGGGGTTATTTGCTTAAAACGTTGCAGGTAGAAAGCCATCGCGTGCAGAAGGTG                                              <  SRR4302533.1946639/94‑1 (MQ=60)
                                                  TTGCAGCGTATTCCAAACCCTGGCGAAGAAGTTCAGGTGGGGGGTTATTTGCTTAAAACGTTGCAGGTAGAAAGCCATCGCGTGCAGAAGGTGC                                             <  SRR4302533.1422236/94‑1 (MQ=60)
                                                  TTGCAGCGTATTCCAAAACCTGGCGAAGAAGTTCAGGTGGGGGGTTATTTGCTTAAAACGTTGCAGGTAGAAAGCCATCGCGTGCAGAAGGTGC                                             >  SRR4302533.3229582/1‑94 (MQ=60)
                                                    GCAGCGTATTCCAAAACCTGGCGAAGAAGTTCAGGTGGGGGGTTATTTGCTTAAAACGTTGCAGGTAGAAAGCCATCGCGTGCAGAAGGTGCAG                                           <  SRR4302533.2605894/94‑1 (MQ=60)
                                                    GCAGCGTATTCCAAAACCTGGCGAAGAAGTTCAGGTGGGGGGTTATTTGCTTAAAACGTTGCAGGTAGAAAGCCATCGCGTGCAGAAGGTGCAG                                           <  SRR4302533.553649/94‑1 (MQ=60)
                                                      AGCGTATTCCAAAACCTGGCGAAGAAGTTCAGGTGGGGGGTTATTTGCTTAAAACGTTGCAGGTAGAAAGCCATCGCGTGCAGAAGGTGCAGAT                                         >  SRR4302533.3433496/1‑94 (MQ=60)
                                                      AGCGTATTCCAAAACCTGGCGAAGAAGTTCAGGTGGGGGGTTATTTGCTTAAAACGTTGCAGGTAGAAAGCCATCGCGTGCAGAAGGTGCAGAT                                         >  SRR4302533.4987706/1‑94 (MQ=60)
                                                      AGCGTATTCCAAAACCTGGCGAAGAAGTTCAGGTGGGGGGTTATTTGCTTAAAACGTTGCAGGTAGAAAGCCATCGCGTGCAGAAGGTGCAGAT                                         <  SRR4302533.59561/94‑1 (MQ=60)
                                                      AGCGTATTCCAAAACCTGGCGAAGAAGTTCAGGTGGGGGGTTATTTGCTTAAAACGTTGCAGGTAGAAAGCCATCGCGTGCAGAAGGTGCAGAT                                         <  SRR4302533.1054670/94‑1 (MQ=60)
                                                      AGCGTATTCCAAAACCTGGCGAAGAAGTTCAGGTGGGGGGTTATTTGCTTAAAACGTTGCAGGTAGAAAGCCATCGCGTGCAGAAGGTGCAGAT                                         >  SRR4302533.1729229/1‑94 (MQ=60)
                                                       GCGTATTCCAAAACCTGGCGAAGAAGTTCAGGTGGGGGGTTATTTGCTTAAAACGTTGCAGGTAGAAAGCCATCGCGTGCAGAAGGTGCAGATT                                        >  SRR4302533.5882749/1‑94 (MQ=60)
                                                        CGTATTCCAAAACCTGGCGAAGAAGTTCAGGTGGGGGGTTATTTGCTTAAAACGTTGCAGGTAGAAAGCCATCGCGTGCAGAAGGTGCAGATTA                                       >  SRR4302533.2315158/1‑94 (MQ=60)
                                                        CGTATTCCAAAACCTGGCGAAGAAGTTCAGGTGGGGGGTTATTTGCTTAAAACGTTGCAGGTAGAAAGCCATCGCGTGCAGAAGGTGCAGATTA                                       <  SRR4302533.4047122/94‑1 (MQ=60)
                                                         GTATTCCAAAACCTGGCGAAGAAGTTCAGGTGGGGGGTTATTTGCTTAAAACGTTGCAGGTAGAAAGCCATCGCGTGCAGAAGGTGCAGATTAT                                      >  SRR4302533.4735949/1‑94 (MQ=60)
                                                         GTATTCCAAAACCTGGCGAAGAAGTTCAGGTGGGGGGTTATTTGCTTAAAACGTTGCAGGTAGAAAGCCATCGCGTGCAGAAGGTGCAGATTAT                                      <  SRR4302533.4374171/94‑1 (MQ=60)
                                                         GTATTCCAAAACCTGGCGAAGAAGTTCAGGTGGGGGGTTATTTGCTTAAAACGTTGCAGGTAGAAAGCCATCGCGTGCAGAAGGTGCAGATTAT                                      >  SRR4302533.5619260/1‑94 (MQ=60)
                                                           ATTCCAAAACCTGGCGAAGAAGTTCAGGTGGGGGGTTATTTGCTTAAAACGTTGCAGGTAGAAAGCCATCGCGTGCAGAAGGTGCAGATTATAC                                    >  SRR4302533.5422945/1‑94 (MQ=60)
                                                            TTCCAAAACCTGGCGAAGAAGTTCAGGTGGGGGGTTATTTGCTTAAAACGTTGCAGGTAGAAAGCCATCGCGTGCAGAAGGTGCAGATTATACC                                   <  SRR4302533.1343588/94‑1 (MQ=60)
                                                            TTCCAAAACCTGGCGAAGAAGTTCAGGTGGGGGGTTATTTGCTTAAAACGTTGCAGGTAGAAAGCCATCGCGTGCAGAAGGTGCAGATTATACC                                   <  SRR4302533.5455219/94‑1 (MQ=60)
                                                             TCCAAAACCTGGCGAAGAAGTTCAGGTGGGGGGTTATTTGCTTAAAACGTTGCAGGTAGAAAGCCATCGCGTGCAGAAGGTGCAGATTATACCG                                  >  SRR4302533.4399151/1‑94 (MQ=60)
                                                             TCCAAAACCTGGCGAAGAAGTTCAGGTGGGGGGTTATTTGCTTAAAACGTTGCAGGTAGAAAGCCATCGCGTGCAGAAGGTGCAGATTATACCG                                  >  SRR4302533.4986744/1‑94 (MQ=60)
                                                              CCAAAACCTGGCGAAGAAGTTCAGGTGGGGGGTTATTTGCTTAAAACGTTGCAGGTAGAAAGCCATCGCGTGCAGAAGGTGCAGATTATACCGC                                 >  SRR4302533.5000573/1‑94 (MQ=60)
                                                              CCAAAACCTGGCGAAGAAGTTCAGGTGGGGGGTTATTTGCTTAAAACGTTGCAGGTAGAAAGCCATCGCGTGCAGAAGGTGCAGATTATACCGC                                 >  SRR4302533.4604792/1‑94 (MQ=60)
                                                              CCAAAACCTGGCGAAGAAGTTCAGGTGGGGGGTTATTTGCTTAAAACGTTGCAGGTAGAAAGCCATCGCGTGCAGAAGGTGCAGATTATACCGC                                 >  SRR4302533.4709774/1‑94 (MQ=60)
                                                                 AAACCTGGCGAAGAAGTTCAGGTGGGGGGTTATTTGCTTAAAACGTTGCAGGTAGAAAGCCATCGCGTGCAGAAGGTGCAGATTATACCGCTGC                              <  SRR4302533.3166783/94‑1 (MQ=60)
                                                                  AACCTGGCGAAGAAGTTCAGGTGGGGGGTTATTTGCTTAAAACGTTGCAGGTAGAAAGCCATCGCGTGCAGAAGGTGCAGATTATACCGCTGCG                             <  SRR4302533.1016257/94‑1 (MQ=60)
                                                                    CCTGGCGAAGAAGTTCAGGTGGGGGGTTATTTGCTTAAAACGTTGCAGGTAGAAAGCCATCGCGTGCAGAAGGTGCAGATTATACCGCTGCGTA                           >  SRR4302533.1862882/1‑94 (MQ=60)
                                                                    CCTGGCGAAGAAGTTCAGGTGGGGGGTTATTTGCTTAAAACGTTGCAGGTAGAAAGCCATCGCGTGCAGAAGGTGCAGATTATACCGCTGCGTA                           >  SRR4302533.810095/1‑94 (MQ=60)
                                                                    CCTGGCGAAGAAGTTCAGGTGGGGGGTTATTTGCTTAAAACGTTGCAGGTAGAAAGCCATCGCGTGCAGAAGGTGCAGATTATACCGCTGCGTA                           >  SRR4302533.4488595/1‑94 (MQ=60)
                                                                    CCTGGCGAAGAAGTTCAGGTGGGGGGTTATTTGCTTAAAACGTTGCAGGTAGAAAGCCATCGCGTGCAGAAGGTGCAGATTATACCGCTGCATA                           >  SRR4302533.556897/1‑94 (MQ=60)
                                                                     CTGGCGAAGAAGTTCAGGTGGGGGGTTATTTGCTTAAAACGTTGCAGGTAGAAAGCCATCGCGTGCAGAAGGTGCAGATTATACCGCTGCGTAA                          >  SRR4302533.5307858/1‑94 (MQ=60)
                                                                     CTGGCGAAGAAGTTCAGGTGGGGGGTTATTTGCTTAAAACGTTGCAGGTAGAAAGCCATCGCGTGCAGAAGGTGCAGATTATACCGCTGCGTAA                          >  SRR4302533.281055/1‑94 (MQ=60)
                                                                       GGCGAAGAAGTTCAGGTGGGGGGTTATTTGCTTAAAACGTTGCAGGTAGAAAGCCATCGCGTGCAGAAGGTGCAGATTATACCGCTGCGTAAGG                        <  SRR4302533.4670445/94‑1 (MQ=60)
                                                                       GGCGAAGAAGTTCAGGTGGGGGGTTATTTGCTTAAAACGTTGCAGGTAGAAAGCCATCGCGTGCAGAAGGTGCAGATTATACCGCTGCGTAAGG                        >  SRR4302533.5585010/1‑94 (MQ=60)
                                                                         CGAAGAAGTTCAGGTGGGGGGTTATTTGCTTAAAACGTTGCAGGTAGAAAGCCATCGCGTGCAGAAGGTGCAGATTATACCGCTGCGTAAGGAT                      >  SRR4302533.2121372/1‑94 (MQ=60)
                                                                          GAAGAAGTTCAGGTGGGGGGTTATTTGCTTAAAACGTTGCAGGTAGAAAGCCATCGCGTGCAGAAGGTGCAGATTATACCGCTGCGTAAGGCTG                     >  SRR4302533.5902920/1‑94 (MQ=60)
                                                                          GAAGAAGTTCAGGTGGGGGGTTATTTGCTTAAAACGTTGCAGGTAGAAAGCCATCGCGTGCAGAAGGTGCAGATTATACCGCTGCGTAAGGATG                     <  SRR4302533.1181400/94‑1 (MQ=60)
                                                                          GAAGAAGTTCAGGTGGGGGGTTATTTGCTTAAAACGTTGCAGGTAGAAAGCCATCGCGTGCAGAAGGTGCAGATTATACCGCTGCGTAAGGATG                     <  SRR4302533.5595765/94‑1 (MQ=60)
                                                                          GAAGAAGTTCAGGTGGGGGGTTATTTGCTTAAAACGTTGCAGGTAGAAAGCCAGCGCGTGCAGAAGGTGCAGATTATGCCGCTGCGTAAGGATG                     <  SRR4302533.71736/94‑1 (MQ=60)
                                                                           AAGAAGTTCAGGTGGGGGGTTATTTGCTTAAAACGTTGCAGGTAGAAAGCCATCGCGTGCAGAAGGTGCAGATTATACCGCTGCGTAAGGATGG                    >  SRR4302533.6271055/1‑94 (MQ=60)
                                                                           AAGAAGTTCAGGTGGGGGGTTATTTGCTTAAAACGTTGCAGGTAGAAAGCCATCGCGTGCAGAAGGTGCAGATTATACCGCTGCGTAAGGATGG                    >  SRR4302533.4510900/1‑94 (MQ=60)
                                                                            AGAAGTTCAGGTGGGGGGTTATTTGCTTAAAACGTTGCAGGTAGAAAGCCATCGCGTGCAGAAGGTGCAGATTATACCGCTGCGTAAGGATGGC                   <  SRR4302533.2881519/94‑1 (MQ=60)
                                                                            AGAAGTTCAGGTGGGGGGTTATTTGCTTAAAACGTTGCAGGTAGAAAGCCATCGCGTGCAGAAGGTGCAGATTATACCGCTGCGTAAGGATGGC                   <  SRR4302533.2441813/94‑1 (MQ=60)
                                                                            AGAAGTTCAGGTGGGGGGTTATTTGCTTAAAACGTTGCAGGTAGAAAGCCATCGCGTGCAGAAGGTGCAGATTATACCGCTGCGTAAGGATGGC                   <  SRR4302533.1593925/94‑1 (MQ=60)
                                                                             GAAGTTCAGGTGGGGGGTTATTTGCTTAAAACGTTGCAGGTAGAAAGCCATCGCGTGCAGAAGGTGCAGATTATACCGCTGCGTAAGGATGGCG                  >  SRR4302533.3866065/1‑94 (MQ=60)
                                                                              AAGTTCAGGTGGGGGGTTATTTGCTTAAAACGTTGCAGGTAGAAAGCCATCGCGTGCAGAAGGTGCAGATTATACCGCTGCGTAAGGATGGCGA                 >  SRR4302533.476585/1‑94 (MQ=60)
                                                                                 TTCAGGTGGGGGGTTATTTGCTTAAAACGTTGCAGGTAGAAAGCCATCGCGTGCAGAAGGTGCAGATTATACCGCTGCGTAAGGATGGCGAGAT              <  SRR4302533.1904973/94‑1 (MQ=60)
                                                                                 TTCAGGTGGGGGGTTATTTGCTTAAAACGTTGCAGGTAGAAAGCCATCGCGTGCAGAAGGTGCAGATTATACCGCTGCGTAAGGATGGCGAGAT              >  SRR4302533.1175509/1‑94 (MQ=60)
                                                                                  TCAGGTGGGGGGTTATTTGCTTAAAACGTTGCAGGTAGAAAGCCATCGCGTGCAGAAGGTGCAGATTATACCGCTGCGTAAGGATGGCGAGATG             <  SRR4302533.775158/94‑1 (MQ=60)
                                                                                   CAGGTGGGGGGTTATTTGCTTAAAACGTTGCAGGTAGAAAGCCATCGCGTGCAGAAGGTGCAGATTATACCGCTGCGTAAGGATGGCGAGATGG            >  SRR4302533.6058665/1‑94 (MQ=60)
                                                                                     GGTGGGGGGTTATTTGCTTAAAACGTTGCAGGTAGAAAGCCATCGCGTGCAGAAGGTGCAGATTATACCGCTGCGTAAGGATGGCGAGATGGAG          >  SRR4302533.3745080/1‑94 (MQ=60)
                                                                                      GTGGGGGGTTATTTGCTTAAAACGTTGCAGGTAGAAAGCCATCGCGTGCAGAAGGTGCAGATTATACCGCTGCGTAAGGATGGCGAGATGGAGT         <  SRR4302533.2225880/94‑1 (MQ=60)
                                                                                       TGGGGGGTTATTTGCTTAAAACGTTGCAGGTAGAAAGCCATCGCGTGCAGAAGGTGCAGATTATACCGCTGCGTAAGGATGGCGAGATGGAGTA        >  SRR4302533.4582273/1‑94 (MQ=60)
                                                                                       TGGGGGGTTATTTGCTTAAAACGTTGCAGGTAGAAAGCCATCGCGTGCAGAAGGTGCAGATTATACCGCTGCGTAAGGATGGCGAGATGGAGTA        >  SRR4302533.2097175/1‑94 (MQ=60)
                                                                                       TGGGGGGTTATTTGCTTAAAACGTTGCAGGTAGAAAGCCATCGCGTGCAGAAGGTGCAGATTATACCGCTGCGTAAGGATGGCGAGATGGAGTA        <  SRR4302533.4428396/94‑1 (MQ=60)
                                                                                       TGGGGGGTGATTTGCTTAAAACGTTGCAGGTAGAAAGCCATCGCGTGCAGAAGGTGCAGATTATACCGCTGCGTAAGGATGGCGAGATGGAGTA        <  SRR4302533.1604956/94‑1 (MQ=60)
                                                                                        GGGGGGTTATTTGCTTAAAACGTTGCAGGTAGAAAGCCATCGCGTGCAGAAGGTGCAGATTATACCGCTGCGTAAGGATGGCGAGATGGAGTAC       <  SRR4302533.825959/94‑1 (MQ=60)
                                                                                         GGGGGTTATTTGCTTAAAACGTTGCAGGTAGAAAGCCATCGCGTGCAGAAGGTGCAGATTATACCGCTGCGTAAGGATGGCGAGATGGAGTACG      >  SRR4302533.4500449/1‑94 (MQ=60)
                                                                                         GGGGGTTATTTGCTTAAAACGTTGCAGGTAGAAAGCCATCGCGTGCAGAAGGTGCAGATTATACCGCTGCGTAAGGATGGCGAGATGGAGTACG      >  SRR4302533.6090050/1‑94 (MQ=60)
                                                                                         GGGGGTTATTTGCTTAAAACGTTGCAGGTAGAAAGCCATCGCGTGCAGAAGGTGCAGATTATACCGCTGCGTAAGGATGGCGAGATGGAGTACG      >  SRR4302533.2870316/1‑94 (MQ=60)
                                                                                           GGGTTATTTGCTTAAAACGTTGCAGGTAGAAAGCCATCGCGTGCAGAAGGTGCAGATTATACCGCTGCGTAAGGATGGCGAGATGGAGTACGAG    <  SRR4302533.2867217/94‑1 (MQ=60)
                                                                                           GGGTTATTTGCTTAAAACGTTGCAGGTAGAAAGCCATCGCGTGCAGAAGGTGCAGATTATACCGCTGCGTAAGGATGGCGAGATGGAGTACGAG    <  SRR4302533.2867218/94‑1 (MQ=60)
                                                                                           GGGTTATTTGCTTAAAACGTTGCAGGTAGAAAGCCATCGCGTGCAGAAGGTGCAGATTATACCGCTGCGTAAGGATGGCGAGATGGAGTACGAG    <  SRR4302533.6188381/94‑1 (MQ=60)
                                                                                            GGTTATTTGCTTAAAACGTTGCAGGTAGAAAGCCATCGCGTGCAGAAGGTGCAGATTATACCGCTGCGTAAGGATGGCGAGATGGAGTACGAGG   >  SRR4302533.3972834/1‑94 (MQ=60)
                                                                                             GTTATTTGCTTAAAACGTTGCAGGTAGAAAGCCATCGCGTGCAGAAGGTGCAGATTATACCGCTGCGTAAGGATGGCGAGATGGAGTACGAGGT  <  SRR4302533.1143256/94‑1 (MQ=60)
                                                                                             GTTATTTGCTTAAAACGTTGCAGGTAGAAAGCCATCGCGTGCAGAAGGTGCAGATTATACCGCTGCGTAAGGATGGCGAGATGGAGTACGAGGT  >  SRR4302533.863903/1‑94 (MQ=60)
                                                                                             GTTATTTGCTTAAAACGTTGCAGGTAGAAAGCCATCGCGTGCAGAAGGTGCAGATTATACCGCTGCGTAAGGATGGCGAGATGGAGTACGAGGT  >  SRR4302533.4173056/1‑94 (MQ=60)
                                                                                             |                                                                                             
TGGATGAAAAACGTGAATATCACACCATTGCCGGGCTGTTGATGGAGTATTTGCAGCGTATTCCAAAACCTGGCGAAGAAGTTCAGGTGGGGGATTATTTGCTTAAAACGTTGCAGGTAGAAAGCCATCGCGTGCAGAAGGTGCAGATTATACCGCTGCGTAAGGATGGCGAGATGGAGTACGAGGT  >  NC_000913/2137317‑2137503

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑