Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,349,989 T→C M136V (ATG→GTG)  glpT ← sn‑glycerol‑3‑phosphate permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,349,9890TC100.0% 530.7 / NA 167M136V (ATG→GTG) glpTsn‑glycerol‑3‑phosphate permease
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (83/84);  total (83/84)

Only 100 of 180 total aligned reads displayed.

TGTGCGCACAGTTCCACACTGACACAATGCCGCCACGTTCTTTCTGCGACCACCAGTGCACCATAGTACGACCACACGGCGGCCACCCCATCCCCTGGAACCAACCGCAGAGGAACAACAGTACAAACATCACCGCAATGCTCGACGTCGCCCATGGCACAAAGCCCATAAACAACATCA  >  NC_000913/2349899‑2350078
                                                                                          |                                                                                         
  tgCGCACAGTTCCACACTGACACAATGCCGCCACGTTCTTTCTGCGACCACCAGTGCACCATAGTACGACCACACGGCGGCCACCCCACcccc                                                                                       <  2:3041624/93‑1 (MQ=255)
  tgCGCACAGTTCCACACTGACACAATGCCGCCACGTTCTTTCTGCGACCACCAGTGCACCATAGTACGACCACACGGCGGCCACCCCACcccc                                                                                       <  1:1795729/93‑1 (MQ=255)
  ggcgcACAGTTCCACACTGACACAATGCCGCCACGTTCCTTCTGCGACCACCAGTGCACCATAGTACGACCACACGGCGGCCACCCCACcccc                                                                                       <  2:4204635/92‑1 (MQ=255)
   gcgcACAGTTCCACACTGACACAATGCCGCCACGTTCTTTCTGCGACCACCAGTGCACCATAGTACGACCACACGGCGGCCACCCCACCCCCt                                                                                      >  1:4057952/1‑93 (MQ=255)
    cgcACAGTTCCACACTGACACAATGCCGCCACGTTCTTTCTGCGACCTCCAGTGCCCCATAGTACGACCACACGGCGGCCACCCCACCCCCTg                                                                                     <  2:5002431/93‑1 (MQ=255)
     gcACAGTTCCACACTGACACAATGCCGCCACGTTCTTTCTGCGACCACCAGTGCACCATAGTACGACCACACGGCGGCCACCCCACCCCCTgg                                                                                    <  2:2775189/93‑1 (MQ=255)
     gcACAGTTCCACACTGACACAATGCCGCCACGTTCTTTCTGCGACCACCAGTGCACCATAGTACGACCACACGGCGGCCACCCCACCCCCTgg                                                                                    >  2:5717856/1‑93 (MQ=255)
      cacaGTTCCACACTGACACAATGCCGCCACGTTCTTTCTGCGACCACCAGTGCACCATAGTACGACCACACGGCGGCCACCCCACCCCCTGGa                                                                                   >  2:769833/1‑93 (MQ=255)
       acaGTTCCACACTGACACAATGCCGCCACGTTCTTTCTGCGACCACCAGTGCACCATAGTACGACCACACGGCGGCCACCCCACCCCCTGGaa                                                                                  >  2:4480107/1‑93 (MQ=255)
        caGTTCCACACTGACACAATGCCGCCACGTTCTTTCTGCGACCACCAGTGCACCATAGTACGACCACACGGCGGCCACCCCACCCCCTGGaac                                                                                 >  2:2706038/1‑93 (MQ=255)
        caGTTCCACACTGACACAATGCCGCCACGTTCTTTCTGCGACCACCAGTGCACCATAGTACGACCACACGGCGGCCACCCCACCCCCTGGaac                                                                                 >  1:2048357/1‑93 (MQ=255)
        caGTTCCACACTGACACAATGCCGCCACGTTCTTTCTGCGACCACCAGTGCACCATAGTACGACCACACGGCGGCCACCCCACCCCCTGGaac                                                                                 >  1:3712714/1‑93 (MQ=255)
        caGTTCCACACTGACACAATGCCGCCACGTTCTTTCTGCGACCACCAGTGCACCATAGTACGACCACACGGCGGCCACCCCACCCCCTGGaac                                                                                 <  1:5786658/93‑1 (MQ=255)
         aGTTCCACACTGACACAATGCCGCCACGTTCTTTCTGCGACCACCAGTGCACCATAGTACGACCACACGGCGGCCACCCCACCCCCTGGaacc                                                                                >  2:6254414/1‑93 (MQ=255)
         aGTTCCACACTGACACAATGCCGCCACGTTCTTTCTGCGACCACCAGTGCACCATAGTACGACCACACGGCGGCCACCCCACCCCCTGGaacc                                                                                <  2:2475213/93‑1 (MQ=255)
            tCCACACTGACACAATGCCGCCACGTTCTTTCTGCGACCACCAGTGCACCATAGTACGACCACACGGCGGCCACCCCACCCCCTGGaaccaac                                                                             <  1:6163067/93‑1 (MQ=255)
            tCCACACTGACACAATGCCGCCACGTTCTTTCTGCGACCACCAGTGCACCATAGTACGACCACACGGCGGCCACCCCACCCCCTGGaaccaac                                                                             <  1:3348511/93‑1 (MQ=255)
            tCCACACTGACACAATGCCGCCACGTTCTTTCTGCGACCACCAGTGCACCATAGTACGACCACACGGCGGCCACCCCACCCCCTGGaaccaac                                                                             >  1:2610574/1‑93 (MQ=255)
             ccACACTGACACAATGCCGCCACGTTCTTTCTGCGACCACCAGTGCACCATAGTACGACCACACGGCGGCCACCCCACCCCCTGGaaccaacc                                                                            <  2:4917081/93‑1 (MQ=255)
                cacTGACACAATGCCGCCACGTTCTTTCTGCGACCACCAGTGCACCATAGTACGACCACACGGCGGCCACCCCACCCCCTGGAACCAACCGCa                                                                         >  1:1552814/1‑93 (MQ=255)
                cacTGACACAATGCCGCCACGTTCTTTCTGCGACCACCAGTGCACCATAGTACGACCACACGGCGGCCACCCCACCCCCTGGAACCAACCGCa                                                                         >  1:5637052/1‑93 (MQ=255)
                   tGACACAATGCCCCCACGTTCTTTCTGCGACCGCCAGTGCACCATAGTACGACCACACGGCGGCCACCCCACCCCCTGGAACCAACCGCagag                                                                      <  1:526812/93‑1 (MQ=255)
                     acacAATGCCGCCACGTTCTTTCTGCGACCACCAGTGCACCATAGTACGACCACACGGCGGCCACCCCACCCCCTGGAACCAACCGCAGAGGa                                                                    >  1:3970393/1‑93 (MQ=255)
                       acaATGCCGCCACGTTCTTTCTGCGACCACCAGTGCACCATAGTACGACCACACGGCGGCCACCCCACCCCCTGGAACCGACCGCAGAGGaac                                                                  >  1:3605185/1‑93 (MQ=255)
                       acaATGCCGCCACGTTCTTTCTGCGACCACCAGTGCACCATAGTACGACCACACGGCGGCCACCCCACCCCCTGGAACCAACCGCAGAGGaac                                                                  >  2:1726008/1‑93 (MQ=255)
                       acaATGCCGCCACGTTCTTTCTGCGACCACCAGTGCACCATAGTACGACCACACGGCGGCCACCCCACCCCCTGGAACCAACCGCAGAGGaaa                                                                  >  1:171144/1‑92 (MQ=255)
                        caATGCCGCCACGTTCTTTCTGCGACCACCAGTGCACCATAGTACGACCACACGGCGGCCACCCCACCCCCTGGAACCAACCGCAGAGGaaca                                                                 <  1:5650965/93‑1 (MQ=255)
                        caATGCCGCCACGTTCTTTCTGCGACCACCAGTGCACCATAGTACGACCACACGGCGGCCACCCCACCCCCTGGAACCAACCGCAGAGGaaca                                                                 >  1:645824/1‑93 (MQ=255)
                         aaTGCCGCCACGTTCTTTCTGCGACCACCAGTGCACCATAGTACGACCACACGGCGGCCACCCCACCCCCTGGAACCAACCGCAGAGGaacaa                                                                <  1:4669048/93‑1 (MQ=255)
                          aTGCCGCCACGTTCTTTCTGCGACCACCAGTGCACCATAGTACGACCACACGGCGGCCACCCCACCCCCTGGAACCAACCGCAGAGGaacaac                                                               >  1:5413159/1‑93 (MQ=255)
                          aTGCCGCCACGTTCTTTCTGCGACCACCAGTGCACCATAGTACGACCACACGGCGGCCACCCCACCCCCTGGAACCAACCGCAGAGGaacaac                                                               <  2:4165960/93‑1 (MQ=255)
                          aTGCCGCCACGTTCTTTCTGCGACCACCAGTGCACCATAGTACGACCACACGGCGGCCACCCCACCCCCTGGAACCAACCGCAGAGGaacaac                                                               >  1:237183/1‑93 (MQ=255)
                          aTGCCGCCACGTTCTTTCTGCGACCACCAGTGCACCATAGTACGACCACACGGCGGCCACCCCACCCCCTGGAACCAACCGCAGAGGaacaac                                                               <  2:5565631/93‑1 (MQ=255)
                           tGCCGCCACGTTCTTTCTGCGACCACCAGTGCACCATAGTACGACCACACGGCGGCCACCCCACCCCCTGGAACCAACCGCAGAGGAacaaca                                                              <  1:1016072/93‑1 (MQ=255)
                           tGCCGCCACGTTCTTTCTGCGACCACCAGTGCACCATAGTACGACCACACGGCGGCCACCCCACCCCCTGGAACCAACCGCAGAGGAacaaca                                                              <  2:6193065/93‑1 (MQ=255)
                                ccACGTTCTTTCTGCGACCACCAGTGCACCATAGTACGACCACACGGCGGCCACCCCACCCCCTGGAACCAACCGCAGAGGAACAACAGTACa                                                         <  2:4800959/93‑1 (MQ=255)
                                 cACGTTCTTTCTGCGACCACCAGTGCACCATAGTACGACCACACGGCGGCCACCCCACCCCCTGGAACCAACCGCAGAGGAACAACAGTACaa                                                        >  2:5973526/1‑93 (MQ=255)
                                  aCGTTCTTTCTGCGACCACCAGTGCACCATAGTACGACCACACGGCGGCCCCCCCACCCCCTGGAACCAACCGCAGAGGGACAACAGTACaaa                                                       >  1:1234977/1‑93 (MQ=255)
                                  aCGTTCTTTCTGCGACCACCAGTGCACCATAGTACGACCACACGGCGGCCACCCCACCCCCTGGAACCAACCGCAGAGGAACAACAGTACaaa                                                       >  1:4177482/1‑93 (MQ=255)
                                  aCGTTCTTTCTGCGACCACCAGTGCACCATAGTACGACCACACGGCGGCCACCCCACCCCCTGGAACCAACCGCAGAGGAACAACAGTACaaa                                                       >  1:2390791/1‑93 (MQ=255)
                                  aCGTTCTTTCTGCGACCACCAGTGCACCATAGTACGACCACACGGCGGCCACCCCACCCCCTGGAACCAACCGCAGAGGAACAACAGTACaaa                                                       >  1:5360389/1‑93 (MQ=255)
                                  aCGTTCTTTCTGCGACCACCAGTGCACCATAGTACGACCACACGGCGGCCACCCCACCCCCTGGAACCAACCGCAGAGGAACAACAGTACaaa                                                       >  1:2341567/1‑93 (MQ=255)
                                   cGTTCTTTCTGCGACCACCAGTGCACCATAGTACGACCACACGGCGGCCACCCCACCCCCTGGAACCAACCGCAGAGGAACAACAGTACAAAc                                                      >  2:4717347/1‑93 (MQ=255)
                                   cGTTCTTTCTGCGACCACCAGTGCACCATAGTACGACCACACGGCGGCCACCCCACCCCCTGGAACCAACCGCAGAGGAACAACAGTACAAAc                                                      >  1:4992564/1‑93 (MQ=255)
                                     ttctttctGCGACCACCAGTGCACCATAGTACGACCACACGGCGGCCACCCCACCCCCTGGAACCAACCGCAGAGGAACAACAGTACAAACAt                                                    >  1:825851/1‑93 (MQ=255)
                                     ttctttctGCGACCACCAGTGCACCATAGTACGACCACACGGCGGCCACCCCACCCCCTGGAACCAACCGCAGAGGAACAACAGTACAAACAt                                                    >  1:6082611/1‑93 (MQ=255)
                                      tctttctGCGACCACCAGTGCACCATAGTACGACCACACGGCGGCCACCCCACCCCCTGGAACCAACCGCAGAGGAACAACAATACAAACATc                                                   >  1:4883427/1‑93 (MQ=255)
                                       ttttctGCGACCACCAGTGCACCATAGTACGACCACACGGCGGCCACCCCACCCCCTGGAACCAACCGCAGAGGAACAACAGTACAAACATCa                                                  <  2:206078/92‑1 (MQ=255)
                                        tttctGCGACCACCAGTGCACCATAGTACGACCACACGGCGGCCACCCCACCCCCTGGAACCAACCGCAGAGGAACAACAGTACAAACATCAc                                                 <  2:267613/93‑1 (MQ=255)
                                        tttctGCGACCACCAGTGCACCATAGTACGACCACACGGCGGCCACCCCACCCCCTGGAACCAACCGCAGAGGAACAACACTACAAACATCAc                                                 >  1:192694/1‑93 (MQ=255)
                                          tctGCGACCACCAGTGCACCATAGTACGACCACACGGCGGCCACCCCACCCCCTGGAACCAACCGCAGAGGAACAACAGTACAAACATCACCg                                               <  1:1091829/93‑1 (MQ=255)
                                             gCGACCACCAGTGCACCATAGTACGACCACACGGCGGCCACCCCACCCCCTGGAACCAACCGCAGAGGAACAACAGTACAAACATCACCGCaa                                            <  2:4517156/93‑1 (MQ=255)
                                              cGACCACCAGTGCACCATAGTACGACCACACGGCGGCCACCCCACCCCCTGGAACCAACCGCAGAGGAACAACAGTACAAACATCACCGCAAt                                           <  2:5580933/93‑1 (MQ=255)
                                              cGACCACCAGTGCACCATAGTACGACCACACGGCGGCCACCCCACCCCCTGGAACCAACCGCAGAGGAACAACAGTACAAACATCACCGCAAt                                           >  1:4707039/1‑93 (MQ=255)
                                               gACCACCAGTGCACCATAGTACGACCACACGGCGGCCACCCCACCCCCTGGAACCAACCGCAGAGGAACAACAGTACAAACATCACCGCAATg                                          >  1:5937204/1‑93 (MQ=255)
                                                accaccAGTGCACCATAGTACGACCACACGGCGGCCACCCCACCCCCTGGAACCAACCGCAGAGGAACAACAGTACAAACATCACCGCAATGc                                         <  2:1116102/93‑1 (MQ=255)
                                                 ccaccaGTGCACCATAGTACGACCACACGGCGGCCACCCCACCCCCTGGAACCAACCGCAGAGGAACAACAGTACAAACATCACCGCAATGCt                                        <  2:2516282/93‑1 (MQ=255)
                                                  caccaGTGCACCATAGTACGACCACACGGCGGCCACCCCACCCCCTGGAACCAACCGCAGAGGAACAACAGTACAAACATCACCGCAATGCTc                                       >  2:5476559/1‑93 (MQ=255)
                                                   accaGTGCACCATAGTACGACCACACGGCGGCCACCCCACCCCCTGGAACCAACCGCAGAGGAACAACAGTACAAACATCACCGCAATGCTCg                                      >  2:1696476/1‑93 (MQ=255)
                                                   accaGTGCACCATAGTACGACCACACGGCGGCCACCCCACCCCCTGGAACCAACCGCAGAGGAACAACAGTACAAACATCACCGCAATGCTCg                                      >  2:3448930/1‑93 (MQ=255)
                                                    ccaGTGCACCATAGTACGACCACACGGCGGCCACCCCACCCCCTGGAACCAACCGCAGAGGAACAACAGTACAAACATCACCGCAATGCTCGa                                     <  1:2848166/93‑1 (MQ=255)
                                                    ccaGTGCACCATAGTACGACCACACGGCGGCCACCCCACCCCCTGGAACCAACCGCAGAGGAACAACAGTACAAACATCACCGCAATGCTCGa                                     <  1:3589152/93‑1 (MQ=255)
                                                    ccaGTGCACCATAGTACGACCACACGGCGGCCACCCCACCCCCTGGAACCAACCGCAGAGGAACAACAGTACAAACATCACCGCAATGCTCGa                                     >  2:3110372/1‑93 (MQ=255)
                                                     caGTGCACCATAGTACGACCACACGGCGGCCACCCCACCCCCTGGAACCAACCGCAGAGGAACAACAGTACAAACATCACCGCAATGCTCGAc                                    >  2:1068058/1‑93 (MQ=255)
                                                      aGTGCACCATAGTACGACCACACGGCGGCCACCCCACCCCCTGGAACCAACCGCAGAGGAACAACAGTACAAACATCACCGCAATGCTCGACg                                   >  1:2782797/1‑93 (MQ=255)
                                                            ccATAGTACGACCACACGGCGGCCACCCCACCCCCTGGAACCAACCGCAGAGGAACCACAGTACAAACATCACCGCCATGCTCGACGTCGccc                             >  2:1444594/1‑93 (MQ=255)
                                                            ccATAGTACGACCACACGGCGGCCACCCCACCCCCTGGAACCAACCGCAGAGGAACAACAGTACAAACATCACCGCAATGCTCGACGTCGccc                             <  2:690953/93‑1 (MQ=255)
                                                            ccATAGTACGACCACACGGCGGCCACCCCACCCCCTGGAACCAACCGCAGAGGAACAACAGTACAAACATCACCGCAATGCTCGACGTCGacc                             >  1:5185780/1‑93 (MQ=255)
                                                            ccATAGTACGACAACCCGGCGGCCACCCCACCCCCTGGAACCAACCGCAGAGGAACAACAGTACAAACATCCCCGCAATGCTCGACGCCGccc                             <  2:117156/93‑1 (MQ=255)
                                                             cATAGTACGACCACACGGCGGCCACCCCACCCCCTGGAACCAACCGCAGAGGAACAACAGTACAAACATCACCGCAATGCTCGACGTCGCCCa                            <  1:2507371/93‑1 (MQ=255)
                                                             cATAGTACGACCACACGGCGGCCACCCCACCCCCTGGAACCAACCGCAGAGGAACAACAGTACAAACATCACCGCAATGCTCGACGTCGCCCa                            <  2:2163696/93‑1 (MQ=255)
                                                               tAGTACGACCGCACGGCGGCCACCCCACCCCCTGGAACCAACCGCAGAGGAACAACAGTACAAACATCACCGCAATGCTCGACGTCGCCCATg                          <  1:3001533/93‑1 (MQ=255)
                                                               tAGTACGACCACACGGCGGCCGCCCCCCCCCCTGGAACCAACCGCAGAGGAACAACAGTACAAACATCACCGCAATGCTCGACGTCGCCCATg                          <  1:1407038/93‑1 (MQ=255)
                                                               tAGTACGACCACACGGCGGCCACCCCACCCCCTGGAACCAACCGCAGAGGAACAACAGTACAAACATCACCGCAATGCTCGACGTCGCCCATg                          <  1:1444941/93‑1 (MQ=255)
                                                               tAGTACGACCACACGGCGGCCACCCCACCCCCTGGAACCAACCGCAGAGGAACAACAGTACAAACATCACCGCAATGCTCGACGTCGCCCATg                          <  1:3148818/93‑1 (MQ=255)
                                                               tAGTACGACCACACGGCGGCCACCCCACCCCCTGGAACCAACCGCAGAGGAACAACAGTACAAACATCACCGCAATGCTCGACGTCGCCCATg                          >  1:4855273/1‑93 (MQ=255)
                                                                aGTACGACCACACGGCGGCCACCCCACCCCCTGGAACCAACCGCAGAGGAACAACAGTACAAACATCACCGCAATGCTCGACGTCGCCCATgg                         >  2:5361741/1‑93 (MQ=255)
                                                                  tACGACCACACGGCGGCCACCCCACCGCCGGGAACCAACCGCAGAGGAACAACAGTACAAACATCACCGCAATGCTCGACGTCGCCCATGGca                       <  1:2002586/93‑1 (MQ=255)
                                                                  tACGACCACACGGCGGCCACCCCACCCCCTGGAACCAACCGCAGAGGAACAACAGTACAAACATCACCGCAATGCTCGACGTCGCCCATGGca                       <  2:511197/93‑1 (MQ=255)
                                                                  tACGACCACACGGCGGCCACCCCACCCCCTGGAACCAACCGCAGAGGAACAACAGTACAAACATCACCGCAATGCTCGACGTCGCCCATGGca                       >  2:755089/1‑93 (MQ=255)
                                                                   aCGACCACACGGCGGCCACCCCACCCCCTGGAACCAACCGCAGAGGAACAACAGTACAAACATCACCGCAATGCTCGACGTCGCCCATGGcac                      >  1:3266428/1‑93 (MQ=255)
                                                                   aCGACCACACGGCGGCCACCCCACCCCCTGGAACCAACCGCAGAGGAACAACAGTACAAACATCACCGCAATGCTCGACGTCGCCCATGGcac                      <  2:5285583/93‑1 (MQ=255)
                                                                     gACCACACGGCGGCCACCCCTCCCCCTGGAACCAACCGCAGAGGAACAACAGTACAAACATCACCGCAATGCTCGACGTCGCCCATGGCACaa                    <  2:2873786/93‑1 (MQ=255)
                                                                      aCCACACGGCGGCCACCCCACCCCCTGGAACCAACCGCAGAGGAACAACAGTACAAACATCACCGCAATGCTCGACGTCGCCCATGGCACaaa                   >  2:2101340/1‑93 (MQ=255)
                                                                        cacaCGGCGGCCACCCCACCCCCTGGAACCAACCGCAGAGGAACAACAGTACAAACATCACCGCAATGCTCGACGTCGCCCATGGCACAAAGc                 >  2:3748525/1‑93 (MQ=255)
                                                                        cacaCGGCGGCCACCCCACCCCCTGGAACCAACCGCAGAGGAACAACAGTACAAACATCACCGCAATGCTCGACGTCGCCCATGGCACAAAGc                 <  2:1354364/93‑1 (MQ=255)
                                                                         acacGGCGGCCACCCCACCCCCTGGAACCAACCGCAGAGGAACAACAGTACAAACATCACCGCAATGCTCGACGTCGCCCATGGCACAAAgac                >  2:583009/1‑91 (MQ=255)
                                                                         acacGGCGGCCACCCCACCCCCTGGAACCAACCGCAGAGGAACAACAGTACAAACATCACCGCAATGCTCGACGTCGCCCATGGCACAAAGcc                >  2:3641456/1‑93 (MQ=255)
                                                                         acacGGCGGCCACCCCACCCCCTGGAACCAACCGCAGAGGAACAACAGTACAAACATCACCGCAATGCTCGACGTCGCCCATGGCACAAAGcc                >  1:984928/1‑93 (MQ=255)
                                                                           acGGCGGCCACCCCCCCCCCTGGAACCAACCGCAGAGGAACAACAGTACAAACATCACCGCAATGCTCGACGTCGCCCATGGCACAAAGCCCa              <  2:5172832/93‑1 (MQ=255)
                                                                           acGGCGGCCACCCCACCCCCTGGAACCAACCGCAGAGGAACAGCAGTACAAACATCACCGCAATGCTCGACGTCGCCCATGGCACAAAGCCCa              >  1:4432207/1‑93 (MQ=255)
                                                                           acGGCGGCCACCCCACCCCCTGGAACCAACCGCAGAGGAACAACAGTACAAACATCACCGCAATGCTCGACGTCGCCCATGGCACAAAGCCCa              >  2:4368594/1‑93 (MQ=255)
                                                                            cggcggCCACCCCACCCCCTGGAACCAACCGCAGAGGAACAACAGTACAAACATCACCGCAATGCTCGACGTCGCCCATGGCACAAAGCCCAt             >  1:1057547/1‑93 (MQ=255)
                                                                            cggcggCCACCCCACCCCCTGGAACCAACCGCAGAGGAACAACAGTACAAACATCACCGCAATGCTCGACGTCGCCCATGGCACAAAGCCCAt             <  1:4465561/93‑1 (MQ=255)
                                                                               cggcCACCCCCCCCCCTGGAACCAACCGCAGAGGAACAACAGTACAAACATCACCGCAATGCTCGACGTCGCCCATGGCACAAAGCCCATaaa          <  1:6105039/93‑1 (MQ=255)
                                                                                  cccccccACCCCCTGGAACCAACCGCAGAGGAACAACAGTACAAACATCACCGCAATGCTCGACGTCGCCCATGGCACAAAGCCCATAaacaa       <  1:557086/90‑1 (MQ=255)
                                                                                    cccccACCCCCTGGAACCAACCGCAGAGGAACAACAGTACAAACATCACCGCAATGCTCGACGTCGCCCATGGCACAAAGCCCATAAacaaca     <  2:4546274/92‑1 (MQ=255)
                                                                                    cccccACCCCCTGGAACCAACCGCAGAGGAACAACAGTACAAACATCACCGCAATGCTCGACGTCGCCCATGGCACAAAGCCCATAAacaaca     <  2:1954288/92‑1 (MQ=255)
                                                                                    accccaccccCTGGAACCAACCGCAGAGGAACAACAGTACAAACATCACCGCAATGCTCGACGTCGCCCATGGCACAAAGCCCATAAacaaca     >  1:4581836/1‑93 (MQ=255)
                                                                                       ccACCCCCTGGAACCAACCGCAGAGGAACAACAGTACAAACATCACCGCAATGCTCGACGTCGCCCATGGCACAAAGCCCATAAACAACATCa  <  2:330547/93‑1 (MQ=255)
                                                                                          |                                                                                         
TGTGCGCACAGTTCCACACTGACACAATGCCGCCACGTTCTTTCTGCGACCACCAGTGCACCATAGTACGACCACACGGCGGCCACCCCATCCCCTGGAACCAACCGCAGAGGAACAACAGTACAAACATCACCGCAATGCTCGACGTCGCCCATGGCACAAAGCCCATAAACAACATCA  >  NC_000913/2349899‑2350078

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CGTTGTGCGCACAGTTCCACACTGACACAATGCCGCCACGTTCTTTCTGCGACCACCAGTGCACCATAGTACGACCACACGGCGGCCACCCCATCCCCTGGAACCAACCGCAGAGGAACAACAGTACAAACATCACCGCAATGCTCGACGTCGCCCATGGCACAAAGCCCATAAACAACATCACTGC  >  NC_000913/2349896‑2350082
                                                                                             |                                                                                             
CGTTGTGCGCACAGTTCCACACTGACACAATGCCGCCACGTTCTTTCTGCGACCACCAGTGCACCATAGTACGACCACACGGCGGGCACCCCAC                                                                                               >  SRR4302533.3978218/1‑94 (MQ=60)
CGTTGTGCGCACAGTTCCACACTGACACAATGCCGCCACGTTCTTTCTGCGACCACCAGTGCACCATAGTACGACCACACGGCGGCCACCCCAC                                                                                               >  SRR4302533.223401/1‑94 (MQ=60)
  TTGTGCGCACAGTTCCACACTGACACAATGCCGCCACGTTCTTTCTGCGACCACCAGTGCACCATAGTACGACCACACGGCGGCCACCCCACCC                                                                                             >  SRR4302533.2382459/1‑94 (MQ=60)
     TGCGCACAGTTCCACACTGACACAATGCCGCCACGTTCTTTCTGCGACCACCAGTGCACCATAGTACGACCACACGGCGGCCACCCCACCCCCT                                                                                          >  SRR4302533.4082653/1‑94 (MQ=60)
     TGCGCACAGTTCCACACTGACACAATGCCGCCACGTTCTTTCTGCGACCACCAGTGCACCATAGTACGACCACACGGCGGCCACCCCACCCCCT                                                                                          <  SRR4302533.1806657/94‑1 (MQ=60)
        GCACAGTTCCACACTGACACAATGCCGCCACGTTCTTTCTGCGACCACCAGTGCACCATAGTACGACCACACGGCGGCCACCCCACCCCCTGGA                                                                                       <  SRR4302533.4743659/94‑1 (MQ=60)
          ACAGTTCCACACTGACACAATGCCGCCACGTTCTTTCTGCGACCACCAGTGCACCATAGTACGACCACACGGCGGCCACCCCACCCCCTGGAAC                                                                                     >  SRR4302533.2060987/1‑94 (MQ=60)
          ACAGTTCCACACTGACACAATGCCGCCACGTTCTTTCTGCGACCACCAGTGCACCATAGTACGACCACACGGCGGCCACCCCACCCCCTGGAAC                                                                                     >  SRR4302533.3735513/1‑94 (MQ=60)
           CAGTTCCACACTGACACAATGCCGCCACGTTCTTTCTGCGACCACCAGTGCACCATAGTACGACCACACGGCGGCCACCCCACCCCCTGGAACC                                                                                    <  SRR4302533.5821612/94‑1 (MQ=60)
            AGTTCCACACTGACACAATGCCGCCACGTTCTTTCTGCGACCACCAGTGCACCATAGTACGACCACACGGCGGCCACCCCACCCCCTGGAACCA                                                                                   >  SRR4302533.3729943/1‑94 (MQ=60)
              TTCCACACTGACACAATGCCGCCACGTTCTTTCTGCGACCACCAGTGCACCATAGTACGACCACACGGCGGCCACCCCACCCCCTGGAACCAAC                                                                                 >  SRR4302533.2626598/1‑94 (MQ=60)
               TCCACACTGACACAATGCCGCCACGTTCTTTCTGCGACCACCAGTGCACCATAGTACGACCACACGGCGGCCACCCCACCCCCTGGAACCAACC                                                                                <  SRR4302533.6200122/94‑1 (MQ=60)
               TCCACACTGACACAATGCCGCCACGTTCTTTCTGCGACCACCAGTGCACCATAGTACGACCACACGGCGGCCACCCCACCCCCTGGAACCAACC                                                                                <  SRR4302533.1412677/94‑1 (MQ=60)
               TCCACACTGACACAATGCCGCCACGTTCTTTCTGCGACCACCAGTGCACCATAGTACGACCACACGGCGGCCACCCCACCCCCTGGAACCAACC                                                                                <  SRR4302533.3369201/94‑1 (MQ=60)
                CCACACTGACACAATGCCGCCACGTTCTTTCTGCGACCACCAGTGCACCATAGTACGACCACACGGCGGCCACCCCACCCCCTGGAACCAACCG                                                                               <  SRR4302533.3450412/94‑1 (MQ=60)
                  ACACTGACACAATGCCGCCACGTTCTTTCTGCGACCACCAGTGCACCATAGTACGACCACACGGCGGCCACCCCACCCCCTGGAACCAACCGCA                                                                             >  SRR4302533.1562165/1‑94 (MQ=60)
                  ACACTGACACAATGCCGCCACGTTCTTTCTGCGACCACCAGTGCACCATAGTACGACCACACGGCGGCCACCCCACCCCCTGGAACCAACCGCA                                                                             >  SRR4302533.5671137/1‑94 (MQ=60)
                   CACTGACACAATGCCGCCACGTTCTTTCTGCGACCACCAGTGCACCATAGTACGACCACACGGCGGCCACCCCACCCCCTGGAACCAACCGCAG                                                                            <  SRR4302533.2741499/94‑1 (MQ=60)
                    ACTGACACAATGCCGCCACGTTCTTTCTGCGACCACCAGTGCACCATAGTACGACCACACGGCGGCCACCCCACCCCCTGGAACCAACCGCAGA                                                                           >  SRR4302533.740089/1‑94 (MQ=60)
                     CTGACACAATGCCGCCACGTTCTTTCTGCGACCACCAGTGCACCATAGTACGACCACACGGCGGCCACCCCACCCCCTGGAACCAACCGCAGAG                                                                          <  SRR4302533.6186602/94‑1 (MQ=60)
                      TGACACAATGCCCCCACGTTCTTTCTGCGACCGCCAGTGCACCATAGTACGACCACACGGCGGCCACCCCACCCCCTGGAACCAACCGCAGAGG                                                                         <  SRR4302533.529934/94‑1 (MQ=60)
                       GACACAATGCCGCCACGTTCTTTCTGCGACCACCAGTGCACCATAGTACGACCACACGGCGGCCACCCCACCCCCTGGAACCAACCGCAGAGGA                                                                        <  SRR4302533.3038178/94‑1 (MQ=60)
                       GACACAATGCCGCCACGTTCTTTCTGCGACCACCAGTGCACCATAGTACGACCACACGGCGGCCACCCCACCCCCTGGAACCAACCGCAGAGGA                                                                        >  SRR4302533.3994570/1‑94 (MQ=60)
                         CACAATGCCGCCACGTTCTTTCTGCGACCACCAGTGCACCATAGTACGACCACACGGCGGCCACCCCACCCCCTGGAACCGACCGCAGAGGAAC                                                                      >  SRR4302533.3627374/1‑94 (MQ=60)
                         CACAATGCCGCCACGTTCTTTCTGCGACCACCAGTGCACCATAGTACGACCACACGGCGGCCACCCCACCCCCTGGAACCAACCGCAGAGGAAA                                                                      >  SRR4302533.172224/1‑94 (MQ=60)
                          ACAATGCCGCCACGTTCTTTCTGCGACCACCAGTGCACCATAGTACGACCACACGGCGGCCACCCCACCCCCTGGAACCAACCGCAGAGGAACA                                                                     >  SRR4302533.649628/1‑94 (MQ=60)
                          ACAATGCCGCCACGTTCTTTCTGCGACCACCAGTGCACCATAGTACGACCACACGGCGGCCACCCCACCCCCTGGAACCAACCGCAGAGGAACA                                                                     >  SRR4302533.4245366/1‑94 (MQ=60)
                           CAATGCCGCCACGTTCTTTCTGCGACCACCAGTGCACCATAGTACGACCACACGGCGGCCACCCCACCCCCTGGAACCAACCGCAGAGGAACAA                                                                    <  SRR4302533.5685123/94‑1 (MQ=60)
                            AATGCCGCCACGTTCTTTCTGCGACCACCAGTGCACCATAGTACGACCACACGGCGGCCACCCCACCCCCTGGAACCAACCGCAGAGGAACAAC                                                                   >  SRR4302533.238650/1‑94 (MQ=60)
                            AATGCCGCCACGTTCTTTCTGCGACCACCAGTGCACCATAGTACGACCACACGGCGGCCACCCCACCCCCTGGAACCAACCGCAGAGGAACAAC                                                                   <  SRR4302533.4697341/94‑1 (MQ=60)
                            AATGCCGCCACGTTCTTTCTGCGACCACCAGTGCACCATAGTACGACCACACGGCGGCCACCCCACCCCCTGGAACCAACCGCAGAGGAACAAC                                                                   >  SRR4302533.5445710/1‑94 (MQ=60)
                              TGCCGCCACGTTCTTTCTGCGACCACCAGTGCACCATAGTACGACCACACGGCGGCCACCCCACCCCCTGGAACCAACCGCAGAGGAACAACAG                                                                 <  SRR4302533.1022045/94‑1 (MQ=60)
                                 CGCCACGTTCTTTCTGCGACCACCAGTGCACCATAGTACGACCACACGGCGGCCACCCCACCCCCTGGAACCAACCGCAGAGGAACAACAGTAC                                                              <  SRR4302533.536508/94‑1 (MQ=60)
                                   CCACGTTCTTTCTGCGACCACCAGTGCACCATAGTACGACCACACGGCGGCCACCCCACCCCCTGGAACCAACCGCAGAGGAACAACAGTACAA                                                            <  SRR4302533.1125863/94‑1 (MQ=60)
                                    CACGTTCTTTCTGCGACCACCAGTGCACCATAGTACGACCACACGGCGGCCCCCCCACCCCCTGGAACCAACCGCAGAGGGACAACAGTACAAA                                                           >  SRR4302533.1242358/1‑94 (MQ=60)
                                    CACGTTCTTTCTGCGACCACCAGTGCACCATAGTACGACCACACGGCGGCCACCCCACCCCCTGGAACCAACCGCAGAGGAACAACAGTACAAA                                                           <  SRR4302533.3018632/94‑1 (MQ=60)
                                    CACGTTCTTTCTGCGACCACCAGTGCACCATAGTACGACCACACGGCGGCCACCCCACCCCCTGGAACCAACCGCAGAGGAACAACAGTACAAA                                                           >  SRR4302533.4202793/1‑94 (MQ=60)
                                    CACGTTCTTTCTGCGACCACCAGTGCACCATAGTACGACCACACGGCGGCCACCCCACCCCCTGGAACCAACCGCAGAGGAACAACAGTACAAA                                                           >  SRR4302533.2405553/1‑94 (MQ=60)
                                    CACGTTCTTTCTGCGACCACCAGTGCACCATAGTACGACCACACGGCGGCCACCCCACCCCCTGGAACCAACCGCAGAGGAACAACAGTACAAA                                                           >  SRR4302533.2356063/1‑94 (MQ=60)
                                    CACGTTCTTTCTGCGACCACCAGTGCACCATAGTACGACCACACGGCGGCCACCCCACCCCCTGGAACCAACCGCAGAGGAACAACAGTACAAA                                                           >  SRR4302533.1425702/1‑94 (MQ=60)
                                    CACGTTCTTTCTGCGACCACCAGTGCACCATAGTACGACCACACGGCGGCCACCCCACCCCCTGGAACCAACCGCAGAGGAACAACAGTACAAA                                                           >  SRR4302533.5392580/1‑94 (MQ=60)
                                     ACGTTCTTTCTGCGACCACCAGTGCACCATAGTACGACCACACGGCGGCCACCCCACCCCCTGGAACCAACCGCAGAGGAACAACAGTACAAAC                                                          >  SRR4302533.5022833/1‑94 (MQ=60)
                                      CGTTCTTTCTGCGACCACCAGTGCACCATAGTACGACCACACGGCGGCCACCCCACCCCCTGGAACCAACCGCAGAGGAACAACAGTACAAACA                                                         >  SRR4302533.5399729/1‑94 (MQ=60)
                                       GTTCTTTCTGCGACCACCAGTGCACCATAGTACGACCACACGGCGGCCACCCCACCCCCTGGAACCAACCGCAGAGGAACAACAGTACAAACAT                                                        >  SRR4302533.830695/1‑94 (MQ=60)
                                       GTTCTTTCTGCGACCACCAGTGCACCATAGTACGACCACACGGCGGCCACCCCACCCCCTGGAACCAACCGCAGAGGAACAACAGTACAAACAT                                                        >  SRR4302533.6119390/1‑94 (MQ=60)
                                       GTTCTTTCTGCGACCACCAGTGCACCATAGTACGACCACACGGCGGCCACCCCACCCCCTGGAACCAACCGCAGAGGAACAACAGTACAAACAT                                                        <  SRR4302533.1588525/94‑1 (MQ=60)
                                        TTCTTTCTGCGACCACCAGTGCACCATAGTACGACCACACGGCGGCCACCCCACCCCCTGGAACCAACCGCAGAGGAACAACAATACAAACATC                                                       >  SRR4302533.4913012/1‑94 (MQ=60)
                                         TCTTTCTGCGACCACCAGTGCACCATAGTACGACCACACGGCGGCCACCCCACCCCCTGGAACCAACCGCAGAGGAACAACAGTACAAACATCA                                                      <  SRR4302533.5684708/94‑1 (MQ=60)
                                          CTTTCTGCGACCACCAGTGCACCATAGTACGACCACACGGCGGCCACCCCACCCCCTGGAACCAACCGCAGAGGAACAACACTACAAACATCAC                                                     >  SRR4302533.193908/1‑94 (MQ=60)
                                           TTTCTGCGACCACCAGTGCACCATAGTACGACCACACGGCGGCCACCCCACCCCCTGGAACCAACCGCAGAGGAACAACAGTACAAACATCACC                                                    <  SRR4302533.447489/94‑1 (MQ=60)
                                             TCTGCGACCACCAGTGCACCATAGTACGACCACACGGCGGCCACCCCACCCCCTGGAACCAACCGCAGAGGAACAACAGTACAAACATCACCGC                                                  <  SRR4302533.1098285/94‑1 (MQ=60)
                                                GCGACCACCAGTGCACCATAGTACGACCACACGGCGGCCACCCCACCCCCTGGAACCAACCGCAGAGGAACAACAGTACAAACATCACCGCAAT                                               <  SRR4302533.6137008/94‑1 (MQ=60)
                                                GCGACCACCAGTGCACCATAGTACGACCACACGGCGGCCACCCCACCCCCTGGAACCAACCGCAGAGGAACAACAGTACAAACATCACCGCAAT                                               >  SRR4302533.4735568/1‑94 (MQ=60)
                                                 CGACCACCAGTGCACCATAGTACGACCACACGGCGGCCACCCCACCCCCTGGAACCAACCGCAGAGGAACAACAGTACAAACATCACCGCAATG                                              >  SRR4302533.5973102/1‑94 (MQ=60)
                                                    CCACCAGTGCACCATAGTACGACCACACGGCGGCCACCCCACCCCCTGGAACCAACCGCAGAGGAACAACAGTACAAACATCACCGCAATGCTC                                           >  SRR4302533.5771270/1‑94 (MQ=60)
                                                     CACCAGTGCACCATAGTACGACCACACGGCGGCCACCCCACCCCCTGGAACCAACCGCAGAGGAACAACAGTACAAACATCACCGCAATGCTCG                                          <  SRR4302533.335881/94‑1 (MQ=60)
                                                      ACCAGTGCACCATAGTACGACCACACGGCGGCCACCCCACCCCCTGGAACCAACCGCAGAGGAACAACAGTACAAACATCACCGCAATGCTCGA                                         >  SRR4302533.4004014/1‑94 (MQ=60)
                                                       CCAGTGCACCATAGTACGACCACACGGCGGCCACCCCGCCCCCTGGAACCAACCGCAGAGGAACAACAGTACAATCATCACCGCAATGCTCGAC                                        <  SRR4302533.2404389/94‑1 (MQ=60)
                                                       CCAGTGCACCATAGTACGACCACACGGCGGCCACCCCACCCCCTGGAACCAACCGCAGAGGAACAACAGTACAAACATCACCGCAATGCTCGAC                                        <  SRR4302533.3611250/94‑1 (MQ=60)
                                                       CCAGTGCACCATAGTACGACCACACGGCGGCCACCCCACCCCCTGGAACCAACCGCAGAGGAACAACAGTACAAACATCACCGCAATGCTCGAC                                        <  SRR4302533.2865691/94‑1 (MQ=60)
                                                       CCAGTGCACCATAGTACGACCACACGGCGGCCACCCCACCCCCTGGAACCAACCGCAGAGGAACAACAGTACAAACATCACCGCAATGCTCGAC                                        >  SRR4302533.2437165/1‑94 (MQ=60)
                                                        CAGTGCACCATAGTACGACCACACGGCGGCCACCCCACCCCCTGGAACCAACCGCAGAGGAACAACAGTACAAACATCACCGCAATGCTCGACG                                       <  SRR4302533.4919593/94‑1 (MQ=60)
                                                        CAGTGCACCATAGTACGACCACACGGCGGCCACCCCACCCCCTGGAACCAACCGCAGAGGAACAACAGTACAAACATCACCGCAATGCTCGACG                                       >  SRR4302533.2799893/1‑94 (MQ=60)
                                                             CACCATAGTACGACCACACGGCGGCCACCCCACCCCCTGGAACCAACCGCAGAGGAACAACAGTACAAACATCACCGCAATGCTCGACGTCGCC                                  >  SRR4302533.1742651/1‑94 (MQ=60)
                                                              ACCATAGTACGACCACACGGCGGCCACCCCACCCCCTGGAACCAACCGCAGAGGAACAACAGTACAAACATCACCGCAATGCTCGACGTCGACC                                 >  SRR4302533.5217081/1‑94 (MQ=60)
                                                                CATAGTACGACCACACGGCGGCCACCCCACCCCCTGGAACCAACCGCAGAGGAACAACAGTACAAACATCACCGCAATGCTCGACGTCGCCCAT                               <  SRR4302533.2522826/94‑1 (MQ=60)
                                                                 ATAGTACGACCACACGGCGGCCACCCCACCCCCTGGAACCAACCGCAGAGGAACAACAGTACAAACATCACCGCAATGCTCGACGTCGCCCATG                              >  SRR4302533.4884697/1‑94 (MQ=60)
                                                                  TAGTACGACCGCACGGCGGCCACCCCACCCCCTGGAACCAACCGCAGAGGAACAACAGTACAAACATCACCGCAATGCTCGACGTCGCCCATGG                             <  SRR4302533.3019888/94‑1 (MQ=60)
                                                                  TAGTACGACCACACGGCGGCCGCCCCCCCCCCTGGAACCAACCGCAGAGGAACAACAGTACAAACATCACCGCAATGCTCGACGTCGCCCATGG                             <  SRR4302533.1415547/94‑1 (MQ=60)
                                                                  TAGTACGACCACACGGCGGCCACCCCACCCCCTGGAACCAACCGCAGAGGAACAACAGTACAAACATCACCGCAATGCTCGACGTCGCCCATGG                             <  SRR4302533.3168177/94‑1 (MQ=60)
                                                                  TAGTACGACCACACGGCGGCCACCCCACCCCCTGGAACCAACCGCAGAGGAACAACAGTACAAACATCACCGCAATGCTCGACGTCGCCCATGG                             <  SRR4302533.1453658/94‑1 (MQ=60)
                                                                   AGTACGACCACACGGCGGCCACCCCACCCCCTGGAACCAACCGCAGAGGAACAACAGTACAAACATCCCCGCAATGCTCGACGTCGCCCATGGC                            <  SRR4302533.1087528/94‑1 (MQ=60)
                                                                   AGTACGACCACACGGCGGCCACCCCACCCCCTGGAACCAACCGCAGAGGAACAACAGTACAAACATCACCGCAATGCTCGACGTCGCCCATGGC                            >  SRR4302533.4694042/1‑94 (MQ=60)
                                                                     TACGACCACACGGCGGCCACCCCACCGCCGGGAACCAACCGCAGAGGAACAACAGTACAAACATCACCGCAATGCTCGACGTCGCCCATGGCAC                          <  SRR4302533.2014921/94‑1 (MQ=60)
                                                                     TACGACCACACGGCGGCCACCCCACCCCCTGGAACCAACCGCAGAGGAACAACAGTACAAACATCACCGCAATGCTCGACGTCGCCCATGGCAC                          >  SRR4302533.3286584/1‑94 (MQ=60)
                                                                      ACGACCACACGGCGGCCACCCCACCCCCTGGAACCAACCGCAGAGGAACAACAGTACAAACATCACCGCAATGCTCGACGTCGCCCATGGCACA                         >  SRR4302533.2066793/1‑94 (MQ=60)
                                                                        GACCACACGGCGGCCACCCCACCCCCTGGAACCAACCGCAGAGGAACAACAGTACAAACATCACCGCAATGCTCGACGTCGCCCATGGCACAAA                       >  SRR4302533.5643883/1‑94 (MQ=60)
                                                                          CCACACGGCGGCCACCCCACCCCCTGGAACCAACCGCAGAGGAACAACAGTACAAACATCACCGCAATGCTCGACGTCGCCCATGGCACAAAGC                     >  SRR4302533.4708040/1‑94 (MQ=60)
                                                                           CACACGGCGGCCACCCCACCCCCTGGAACCAACCGCAGAGGAACAACAGTACAAACATCACCGCAATGCTCGACGTCGCCCATGGCACAAAGCC                    >  SRR4302533.990697/1‑94 (MQ=60)
                                                                             CACGGCGGCCACCCCACCCCCTGGAACCAACCGCAGAGGAACAGCAGTACAAACATCACCGCAATGCTCGACGTCGCCCATGGCACAAAGCCCA                  >  SRR4302533.4459000/1‑94 (MQ=60)
                                                                              ACGGCGGCCACCCCACCCCCTGGAACCAACCGCAGAGGAACAACAGTACAAACATCACCGCAATGCTCGACGTCGCCCATGGCACAAAGCCCAT                 >  SRR4302533.1063764/1‑94 (MQ=60)
                                                                              ACGGCGGCCACCCCACCCCCTGGAACCAACCGCAGAGGAACAACAGTACAAACATCACCGCAATGCTCGACGTCGCCCATGGCACAAAGCCCAT                 <  SRR4302533.1990588/94‑1 (MQ=60)
                                                                               CGGCGGCCACCCCACCCCCTGGAACCAACCGCAGAGGAACAACAGTACAAACATCACCGCAATGCTCGACGTCGCCCATGGCACAAAGCCCATA                <  SRR4302533.4492570/94‑1 (MQ=60)
                                                                                GGCGGCCACCCCACCCCCTGGAACCAACCGCAGAGGAACAACAGTACAAACATCACCGCAATGCTCGACGTCGCCCATGGCACAAAGCCCATAA               <  SRR4302533.5102162/94‑1 (MQ=60)
                                                                                 GCGGCCACCCCACCCCCTGGAACCAACCGCAGAGGAACAACAGTACAAACATCACCGCAATGCTCGACGTCGCCCATGGCACAAAGCCCATAAA              <  SRR4302533.4149118/94‑1 (MQ=60)
                                                                                  CGGCCACCCCCCCCCCTGGAACCAACCGCAGAGGAACAACAGTACAAACATCACCGCAATGCTCGACGTCGCCCATGGCACAAAGCCCATAAAC             <  SRR4302533.6141905/94‑1 (MQ=60)
                                                                                   GGCCACCCCACCCCCTGGAACCAACCGCAGAGGAACAACAGTACAAACATCACCGCAATGCTCGACGTCGCCCATGGCACAAAGCCCATAAACA            <  SRR4302533.5877172/94‑1 (MQ=60)
                                                                                     CCCCCCCACCCCCTGGAACCAACCGCAGAGGAACAACAGTACAAACATCACCGCAATGCTCGACGTCGCCCATGGCACAAAGCCCATAAACAAC          <  SRR4302533.560391/94‑1 (MQ=60)
                                                                                     CCACCCCACCCCCTGGAACCAACCGCAGAGGAACAACAGTACAAACATCACCGCAATGCTCGACGTCGCCCATGGCACAAAGCCCATAAACAAC          >  SRR4302533.2845095/1‑94 (MQ=60)
                                                                                      CACCCCACCCCCTGGAACCAACCGCAGAGGAACAACAGTACAAACATCACCGCAATGCTCGACGTCGCCCATGGCACAAAGCCCATAAACAACA         >  SRR4302533.1809658/1‑94 (MQ=60)
                                                                                      CACCCCACCCCCTGGAACCAACCGCAGAGGAACAACAGTACAAACATCACCGCAATGCTCGACGTCGCCCATGGCACAAAGCCCATAAACAACA         >  SRR4302533.4609597/1‑94 (MQ=60)
                                                                                      CACCCCACCCCCTGGAACCAACCGCAGAGGAACAACAGTACAAACATCACCGCAATGCTCGACGTCGCCCATGGCACAAAGCCCATAAACAACA         <  SRR4302533.6258683/94‑1 (MQ=60)
                                                                                       ACCCCACCCCCTGGAACCAACCGCAGAGGAACAACAGTACAAACATCACCGCAATGCTCGACGTCGCCCATGGCACAAAGCCCATAAACAACAT        <  SRR4302533.361516/94‑1 (MQ=60)
                                                                                       ACCCCACCCCCTGGAACCAACCGCAGAGGAACAACAGTACAAACATCACCGCAATGCTCGACGTCGCCCATGGCACAAAGCCCATAAACAACAT        >  SRR4302533.5260564/1‑94 (MQ=60)
                                                                                        CCCCCCCCCCTGGAACCAACCGCAGAGGAACAACAGTACAAACATCACCGCAATGCTCGACGTCGCCCATGGCACAAAGCCCATAAACAACATC       <  SRR4302533.2781878/94‑1 (MQ=60)
                                                                                           CACCCCCTGGAACCAACCGCAGAGGAACAACAGTACAAACATCACCGCAATGCTCGACGTCGCCCATGGCACAAAGCCCATAAACAACATCACT    <  SRR4302533.51551/94‑1 (MQ=60)
                                                                                            ACCCCCTGGAACCAACCGCAGAGGAACAACAGTACAAACATCACCGCAATGCTCGACGTCGCCCATGGCACAAAGCCCATAAACAACATCACTG   >  SRR4302533.6201839/1‑94 (MQ=60)
                                                                                             CCCCCTGGAACCAACCGCAGAGGAACAACAGTACAAACATCACCGCAATGCTCGACGTCGCCCATGGCACAAAGCCCATAAACAACATCACTGC  >  SRR4302533.2195353/1‑94 (MQ=60)
                                                                                             |                                                                                             
CGTTGTGCGCACAGTTCCACACTGACACAATGCCGCCACGTTCTTTCTGCGACCACCAGTGCACCATAGTACGACCACACGGCGGCCACCCCATCCCCTGGAACCAACCGCAGAGGAACAACAGTACAAACATCACCGCAATGCTCGACGTCGCCCATGGCACAAAGCCCATAAACAACATCACTGC  >  NC_000913/2349896‑2350082

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑