Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,425,773 A→G G13G (GGT→GGC)  argT ← ArgT

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,425,7730AG100.0% 719.6 / NA 227G13G (GGT→GGC) argTArgT
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (105/122);  total (105/122)

Only 100 of 234 total aligned reads displayed.

ATCTTTCGATGAGAACGGTGCGTAGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGACCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGCTTTTTTATGTTGTGTATTGTGTTGTG  >  NC_000913/2425683‑2425861
                                                                                          |                                                                                        
aTCTTTCGATGAGAACGGTGCGTAGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGcc                                                                                        >  2:549541/1‑93 (MQ=255)
 tCTTTCGATGAGAACGGTGCGTAGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCg                                                                                       >  2:3602745/1‑93 (MQ=255)
 tCTTTCGATGAGAACGGTGCGTAGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCg                                                                                       >  2:3906019/1‑93 (MQ=255)
 tCTTTCGATGAGAACGGTGCGTAGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCg                                                                                       >  1:1014393/1‑93 (MQ=255)
   tttCGATGAGAACGGTGCGTAGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGAc                                                                                     <  1:4616821/93‑1 (MQ=255)
   tttCGATGAGAACGGTGCGTAGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGAc                                                                                     <  1:3872392/93‑1 (MQ=255)
   tttCGATGAGAACGGTGCGTAGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGAc                                                                                     >  1:3575705/1‑93 (MQ=255)
   tttCGATGAGAACGGTGCGTAGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGAc                                                                                     >  1:2947764/1‑93 (MQ=255)
      cGATGAGAACGGTGCGTAGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTaa                                                                                  >  2:935493/1‑93 (MQ=255)
      cGATGAGAACGGTGCGTAGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTaa                                                                                  <  2:4107860/93‑1 (MQ=255)
      cGATGAGAACGGTGCGTAGGTGGTATCGGTTCCGATACGTACAGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTaa                                                                                  <  2:3242344/93‑1 (MQ=255)
        aTGAGAACGGTGCGTAGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACa                                                                                >  1:833389/1‑93 (MQ=255)
         tGAGAACGGTGCGTAGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACaa                                                                               <  1:1833461/93‑1 (MQ=255)
         tGAGAACGGTGCGTAGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACaa                                                                               >  1:5088080/1‑93 (MQ=255)
         tGAGAACGGTGCGTAGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACaa                                                                               >  1:5254169/1‑93 (MQ=255)
          gagaACGGTGCGTAGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACaaa                                                                              <  2:1818843/93‑1 (MQ=255)
           agaACGGTGCGTAGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAg                                                                             >  1:2878205/1‑93 (MQ=255)
            gaACGGTGCGTAGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGa                                                                            <  2:2851835/93‑1 (MQ=255)
             aaCGGTGCGTAGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGAc                                                                           <  2:5930949/93‑1 (MQ=255)
              aCGGTGCGTAGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACa                                                                          <  2:3863970/93‑1 (MQ=255)
                ggTGCGTAGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACaga                                                                        >  1:5067999/1‑93 (MQ=255)
                ggTGCGTAGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACaga                                                                        <  1:1122351/93‑1 (MQ=255)
                ggTGCGTAGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACaga                                                                        <  2:5019286/93‑1 (MQ=255)
                ggTGCGTAGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACaga                                                                        <  1:4288066/93‑1 (MQ=255)
                 gTGCGTAGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACagag                                                                       <  1:3652218/93‑1 (MQ=255)
                  tGCGTAGGTGGTATCTGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGc                                                                      <  1:142835/93‑1 (MQ=255)
                    cGTAGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCga                                                                    <  1:3049318/93‑1 (MQ=255)
                    cGTAGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCga                                                                    <  2:4281424/93‑1 (MQ=255)
                    cGTAGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCga                                                                    >  2:626366/1‑93 (MQ=255)
                      tAGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCgaga                                                                  >  2:1449541/1‑93 (MQ=255)
                       aGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGaa                                                                 <  2:491400/93‑1 (MQ=255)
                       aGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGaa                                                                 >  2:3000086/1‑93 (MQ=255)
                        ggtggtATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAAt                                                                >  2:2731601/1‑93 (MQ=255)
                          tggtATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCg                                                              >  2:5973760/1‑93 (MQ=255)
                             tATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGAct                                                           <  1:1349498/93‑1 (MQ=255)
                             tATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGAct                                                           <  1:5525673/93‑1 (MQ=255)
                              aTCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGActt                                                          <  2:2054222/93‑1 (MQ=255)
                              aTCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGActt                                                          >  1:19887/1‑93 (MQ=255)
                              aTCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGActt                                                          <  2:3742767/93‑1 (MQ=255)
                               tCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGActtc                                                         >  1:1369921/1‑93 (MQ=255)
                                 ggTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGActtctt                                                       <  2:3602979/93‑1 (MQ=255)
                                  gTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACttcttc                                                      >  1:2332209/1‑93 (MQ=255)
                                  gTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACttcttc                                                      >  1:610211/1‑93 (MQ=255)
                                   ttCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTca                                                     <  2:6295193/93‑1 (MQ=255)
                                    tCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTcat                                                    >  1:5132452/1‑93 (MQ=255)
                                      cGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTcatac                                                  <  2:4560314/93‑1 (MQ=255)
                                       gATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTcataca                                                 >  2:125615/1‑93 (MQ=255)
                                        aTACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTcatacat                                                >  2:3128564/1‑93 (MQ=255)
                                        aTACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTcatacat                                                >  1:4356594/1‑93 (MQ=255)
                                        aTACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTcatacat                                                >  2:4976093/1‑93 (MQ=255)
                                         tACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTcatacata                                               >  1:1592848/1‑93 (MQ=255)
                                         tACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTcatacata                                               <  2:2481698/93‑1 (MQ=255)
                                          aCGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTcatacatac                                              >  2:2883812/1‑92 (MQ=255)
                                          aCGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATaa                                              <  1:3794665/93‑1 (MQ=255)
                                           cGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAAc                                             >  2:2669702/1‑93 (MQ=255)
                                            gTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAAcc                                            >  1:1087588/1‑93 (MQ=255)
                                              aCCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCt                                          >  1:1762396/1‑93 (MQ=255)
                                                cGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCa                                        >  1:2161060/1‑93 (MQ=255)
                                                 gTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCaa                                       >  1:1623183/1‑93 (MQ=255)
                                                 gTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCaa                                       >  2:4622956/1‑93 (MQ=255)
                                                 gTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCaa                                       <  1:5368166/93‑1 (MQ=255)
                                                 gTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCaa                                       <  2:5291373/93‑1 (MQ=255)
                                                  tctcCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAg                                      >  2:1680269/1‑93 (MQ=255)
                                                  tctcCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAg                                      >  2:6074756/1‑93 (MQ=255)
                                                  tctcCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAg                                      <  2:6193891/93‑1 (MQ=255)
                                                  tctcCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAg                                      <  1:4903989/93‑1 (MQ=255)
                                                  tctcCGGCAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAg                                      >  1:5741833/1‑93 (MQ=255)
                                                    tcCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTg                                    <  1:2433990/93‑1 (MQ=255)
                                                    tcCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTg                                    <  1:2551791/93‑1 (MQ=255)
                                                    tcCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTg                                    >  2:214266/1‑93 (MQ=255)
                                                    tcCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTg                                    <  1:54521/93‑1 (MQ=255)
                                                        gTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGaaaa                                <  2:480569/93‑1 (MQ=255)
                                                        gTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGaaaa                                >  2:5173519/1‑93 (MQ=255)
                                                        gTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGaaaa                                <  2:2082322/93‑1 (MQ=255)
                                                         tAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATACCCCTCAAGTGAAAAt                               <  2:460196/93‑1 (MQ=255)
                                                         tAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAAt                               <  1:3202062/93‑1 (MQ=255)
                                                         tAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAAt                               <  1:2236063/93‑1 (MQ=255)
                                                          aGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATg                              <  2:5576865/93‑1 (MQ=255)
                                                           gcgcCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATgg                             <  1:5012697/93‑1 (MQ=255)
                                                             gccgcATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGCt                           >  1:3433579/1‑93 (MQ=255)
                                                               cgcATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGCttt                         <  1:4792045/93‑1 (MQ=255)
                                                                gcATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGCtttt                        >  2:1378897/1‑93 (MQ=255)
                                                                    aGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGCTTTTTTAt                    <  1:6256110/93‑1 (MQ=255)
                                                                    aGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGCTTTTTTAt                    <  1:3586863/93‑1 (MQ=255)
                                                                     gCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGCTTTTTTAtg                   <  2:5896470/93‑1 (MQ=255)
                                                                      cTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGCTTTTTTAtgt                  >  1:2018275/1‑93 (MQ=255)
                                                                       tGGACGGCGATGTGGATAGGCCGCCTAACAAAGACAGAGAGAGAATCGACTTCTTCATACATACCCCTCAAGTGAAAATGGCTTTTTTAtgtt                 <  1:620308/93‑1 (MQ=255)
                                                                        ggAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGCTTTTTTAtgttg                <  2:3149483/93‑1 (MQ=255)
                                                                        ggAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGCTTTTTTAtgttg                <  2:1282714/93‑1 (MQ=255)
                                                                         gAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGCTTTTTTAtgttgt               >  1:1449687/1‑93 (MQ=255)
                                                                         gAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGCCTTTTTTAtgttgt               >  1:1073481/1‑93 (MQ=255)
                                                                          aaGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGCTTTTTTATGTtgtg              <  2:5113945/93‑1 (MQ=255)
                                                                          aaGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGCTTTTTTATGTtgtg              <  1:5234161/93‑1 (MQ=255)
                                                                           aGCCGCTGTGGATAGTCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGCTTTTTTATGTTgtgt             <  1:3261452/93‑1 (MQ=255)
                                                                            gCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGCTTTTTTATGTTgtgtt            >  1:4338062/1‑92 (MQ=255)
                                                                            gCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGCTTTTTTATGTTGTGTa            <  2:552260/93‑1 (MQ=255)
                                                                               gCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGCTTTTTTATGTTGTGTAttg         >  1:1492826/1‑93 (MQ=255)
                                                                                   tgGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGCTTTTTTATGTTGTGTAttgtgtt     >  1:4162174/1‑93 (MQ=255)
                                                                                     gagaggCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGCTTTTTTATGTTGTGTAttgtgttgt   <  1:2119103/93‑1 (MQ=255)
                                                                                      agaggCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGCTTTTTTATGTTGTGTAttgtgttgtg  <  2:4192724/93‑1 (MQ=255)
                                                                                          |                                                                                        
ATCTTTCGATGAGAACGGTGCGTAGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGACCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGCTTTTTTATGTTGTGTATTGTGTTGTG  >  NC_000913/2425683‑2425861

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CATCTTTCGATGAGAACGGTGCGTAGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGACCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGCTTTTTTATGTTGTGTATTGTGTTGTGTGTTT  >  NC_000913/2425682‑2425866
                                                                                           |                                                                                             
CATCTTTCGATGAGAACGGTGCGTAGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCC                                                                                             >  SRR4302533.6142419/1‑94 (MQ=60)
CATCTTTCGATGAGAACGGTGCGTAGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCC                                                                                             <  SRR4302533.1904978/94‑1 (MQ=60)
 ATCTTTCGATGAGAACGGTGCGTAGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCG                                                                                            >  SRR4302533.1020336/1‑94 (MQ=60)
   CTTTCGATGAGAACGGTGCGTAGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGAC                                                                                          >  SRR4302533.3597745/1‑94 (MQ=60)
   CTTTCGATGAGAACGGTGCGTAGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGAC                                                                                          >  SRR4302533.2965793/1‑94 (MQ=60)
    TTTCGATGAGAACGGTGCGTAGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACT                                                                                         <  SRR4302533.4644777/94‑1 (MQ=60)
    TTTCGATGAGAACGGTGCGTAGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACT                                                                                         <  SRR4302533.3896027/94‑1 (MQ=60)
      TCGATGAGAACGGTGCGTAGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAA                                                                                       >  SRR4302533.1450165/1‑94 (MQ=60)
        GATGAGAACGGTGCGTAGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACA                                                                                     >  SRR4302533.838272/1‑94 (MQ=60)
        GATGAGAACGGTGCGTAGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACA                                                                                     >  SRR4302533.2502544/1‑94 (MQ=60)
        GATGAGAACGGTGCGTAGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACA                                                                                     >  SRR4302533.2750553/1‑94 (MQ=60)
         ATGAGAACGGTGCGTAGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAA                                                                                    <  SRR4302533.2137928/94‑1 (MQ=60)
         ATGAGAACGGTGCGTAGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAA                                                                                    >  SRR4302533.5285734/1‑94 (MQ=60)
         ATGAGAACGGTGCGTAGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAA                                                                                    >  SRR4302533.5118824/1‑94 (MQ=60)
          TGAGAACGGTGCGTAGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAA                                                                                   <  SRR4302533.4335850/94‑1 (MQ=60)
          TGAGAACGGTGCGTAGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAA                                                                                   <  SRR4302533.1844651/94‑1 (MQ=60)
           GAGAACGGTGCGTAGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAG                                                                                  >  SRR4302533.2895914/1‑94 (MQ=60)
            AGAACGGTGCGTAGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCTCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGA                                                                                 <  SRR4302533.3404346/94‑1 (MQ=60)
            AGAACGGTGCGTAGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGACGACTAACAAAGA                                                                                 >  SRR4302533.5671112/1‑94 (MQ=60)
             GAACGGTGCGTAGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGAC                                                                                <  SRR4302533.1613469/94‑1 (MQ=60)
                CGGTGCGTAGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGA                                                                             >  SRR4302533.5098604/1‑94 (MQ=60)
                CGGTGCGTAGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGA                                                                             <  SRR4302533.3660071/94‑1 (MQ=60)
                 GGTGCGTAGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAG                                                                            >  SRR4302533.5272226/1‑94 (MQ=60)
                 GGTGCGTAGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAG                                                                            >  SRR4302533.4227412/1‑94 (MQ=60)
                 GGTGCGTAGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAG                                                                            <  SRR4302533.4313851/94‑1 (MQ=60)
                 GGTGCGTAGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAG                                                                            <  SRR4302533.1129013/94‑1 (MQ=60)
                  GTGCGTAGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGC                                                                           >  SRR4302533.5071559/1‑94 (MQ=60)
                  GTGCGTAGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGC                                                                           <  SRR4302533.3674674/94‑1 (MQ=60)
                   TGCGTAGGTGGTATCTGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCG                                                                          <  SRR4302533.143746/94‑1 (MQ=60)
                   TGCGTAGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCG                                                                          >  SRR4302533.5489413/1‑94 (MQ=60)
                     CGTAGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAG                                                                        <  SRR4302533.5868800/94‑1 (MQ=60)
                     CGTAGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAG                                                                        <  SRR4302533.3067982/94‑1 (MQ=60)
                         GGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATC                                                                    >  SRR4302533.3254415/1‑94 (MQ=60)
                             GTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACT                                                                <  SRR4302533.1676222/94‑1 (MQ=60)
                              TATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTT                                                               <  SRR4302533.1357615/94‑1 (MQ=60)
                              TATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTT                                                               >  SRR4302533.20024/1‑94 (MQ=60)
                              TATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTT                                                               <  SRR4302533.5559031/94‑1 (MQ=60)
                               GTCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTC                                                              >  SRR4302533.5106653/1‑94 (MQ=60)
                               ATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTC                                                              >  SRR4302533.1378155/1‑94 (MQ=60)
                                TCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCT                                                             <  SRR4302533.6176797/94‑1 (MQ=60)
                                 CGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTT                                                            <  SRR4302533.2128857/94‑1 (MQ=60)
                                  GGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTC                                                           >  SRR4302533.2346648/1‑94 (MQ=60)
                                  GGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTC                                                           >  SRR4302533.613799/1‑94 (MQ=60)
                                   GTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCA                                                          >  SRR4302533.4290454/1‑94 (MQ=60)
                                    TTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCAT                                                         >  SRR4302533.5163457/1‑94 (MQ=60)
                                     TCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATA                                                        <  SRR4302533.624687/94‑1 (MQ=60)
                                      CCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATAC                                                       >  SRR4302533.1002452/1‑94 (MQ=60)
                                      CCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATAC                                                       >  SRR4302533.5215569/1‑94 (MQ=60)
                                      CCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATAC                                                       <  SRR4302533.5351799/94‑1 (MQ=60)
                                       CGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACA                                                      >  SRR4302533.2897739/1‑94 (MQ=60)
                                        GATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACAT                                                     >  SRR4302533.5161523/1‑94 (MQ=60)
                                        GATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACAT                                                     >  SRR4302533.1567280/1‑94 (MQ=60)
                                        GATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACAT                                                     >  SRR4302533.4382890/1‑94 (MQ=60)
                                        GATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACAT                                                     <  SRR4302533.1920260/94‑1 (MQ=60)
                                         ATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATA                                                    >  SRR4302533.1602486/1‑94 (MQ=60)
                                          TACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAA                                                   <  SRR4302533.2015401/94‑1 (MQ=60)
                                          TACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAA                                                   <  SRR4302533.3802502/94‑1 (MQ=60)
                                           ACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAAC                                                  >  SRR4302533.3754047/1‑94 (MQ=60)
                                           ACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAAC                                                  <  SRR4302533.4137475/94‑1 (MQ=60)
                                           ACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAAC                                                  <  SRR4302533.3817887/94‑1 (MQ=60)
                                            CGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACC                                                 >  SRR4302533.1093996/1‑94 (MQ=60)
                                             GTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCC                                                <  SRR4302533.5140884/94‑1 (MQ=60)
                                              TACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCT                                               <  SRR4302533.4999874/94‑1 (MQ=60)
                                              TACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCT                                               >  SRR4302533.1773133/1‑94 (MQ=60)
                                               ACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTC                                              >  SRR4302533.296599/1‑94 (MQ=60)
                                               ACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTC                                              <  SRR4302533.560573/94‑1 (MQ=60)
                                                CCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCA                                             <  SRR4302533.1479890/94‑1 (MQ=60)
                                                CCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCA                                             <  SRR4302533.3566529/94‑1 (MQ=60)
                                                CCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCA                                             >  SRR4302533.2174361/1‑94 (MQ=60)
                                                CCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCA                                             >  SRR4302533.1123226/1‑94 (MQ=60)
                                                 CGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAA                                            >  SRR4302533.1633014/1‑94 (MQ=60)
                                                 CGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAA                                            >  SRR4302533.1080588/1‑94 (MQ=60)
                                                  GTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAG                                           <  SRR4302533.5400414/94‑1 (MQ=60)
                                                  GTCTCCGGCAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAG                                           >  SRR4302533.5776483/1‑94 (MQ=60)
                                                   TCTCCGGTGGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGT                                          <  SRR4302533.4552547/94‑1 (MQ=60)
                                                   TCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGT                                          <  SRR4302533.4933705/94‑1 (MQ=60)
                                                    CTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTG                                         <  SRR4302533.3146851/94‑1 (MQ=60)
                                                     TCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGA                                        <  SRR4302533.1806285/94‑1 (MQ=60)
                                                     TCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGA                                        <  SRR4302533.2567494/94‑1 (MQ=60)
                                                     TCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGA                                        <  SRR4302533.2449027/94‑1 (MQ=60)
                                                     TCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGA                                        <  SRR4302533.54890/94‑1 (MQ=60)
                                                      CCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAA                                       <  SRR4302533.3463874/94‑1 (MQ=60)
                                                          TAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATG                                   <  SRR4302533.3221768/94‑1 (MQ=60)
                                                          TAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATG                                   <  SRR4302533.2249887/94‑1 (MQ=60)
                                                          TAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATG                                   <  SRR4302533.1542339/94‑1 (MQ=60)
                                                            GCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGC                                 <  SRR4302533.5043070/94‑1 (MQ=60)
                                                             CGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGCT                                >  SRR4302533.3454793/1‑94 (MQ=60)
                                                             CGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGCT                                >  SRR4302533.1104044/1‑94 (MQ=60)
                                                             CGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGCT                                >  SRR4302533.1420779/1‑94 (MQ=60)
                                                              GCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGCTT                               >  SRR4302533.1228234/1‑94 (MQ=60)
                                                              GCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGCTT                               >  SRR4302533.2686414/1‑94 (MQ=60)
                                                               CCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGCTTT                              <  SRR4302533.4381318/94‑1 (MQ=60)
                                                               CCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGCTTT                              <  SRR4302533.6327090/94‑1 (MQ=60)
                                                               CCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGCTTT                              >  SRR4302533.2199534/1‑94 (MQ=60)
                                                                CGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGCTTTT                             <  SRR4302533.5002277/94‑1 (MQ=60)
                                                                CGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGCTTTT                             <  SRR4302533.4821081/94‑1 (MQ=60)
                                                                CGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGCTTTT                             >  SRR4302533.3104309/1‑94 (MQ=60)
                                                                    TAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGCTTTTTTAT                         <  SRR4302533.2329180/94‑1 (MQ=60)
                                                                     AGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGCTTTTTTATG                        <  SRR4302533.3608952/94‑1 (MQ=60)
                                                                     AGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGCTTTTTTATG                        <  SRR4302533.3608951/94‑1 (MQ=60)
                                                                     AGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGCTTTTTTATG                        >  SRR4302533.4676665/1‑94 (MQ=60)
                                                                     AGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGCTTTTTTATG                        <  SRR4302533.6293521/94‑1 (MQ=60)
                                                                      GCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGCTTTTTTATGT                       >  SRR4302533.2030711/1‑94 (MQ=60)
                                                                      GCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGCTTTTTTATGT                       <  SRR4302533.1809100/94‑1 (MQ=60)
                                                                         GGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGCTTTTTTATGTTGT                    >  SRR4302533.1458435/1‑94 (MQ=60)
                                                                         GGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGCCTTTTTTATGTTGT                    >  SRR4302533.1079809/1‑94 (MQ=60)
                                                                           AAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGCTTTTTTATGTTGTGT                  <  SRR4302533.5265637/94‑1 (MQ=60)
                                                                           AAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGCTTTTTTATGTTGTGT                  <  SRR4302533.2323589/94‑1 (MQ=60)
                                                                           AAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGCTTTTTTATGTTGTGT                  >  SRR4302533.1056594/1‑94 (MQ=60)
                                                                            AGCCGCTGTGGATAGTCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGCTTTTTTATGTTGTGTA                 <  SRR4302533.3281570/94‑1 (MQ=60)
                                                                            AGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGCTTTTTTATGTTGTGTT                 >  SRR4302533.4364209/1‑94 (MQ=60)
                                                                            AGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGCTTTTTTATGTTGTGTA                 >  SRR4302533.1365993/1‑94 (MQ=60)
                                                                             GCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGCTTTTTTATGTTGTGTAT                <  SRR4302533.5446684/94‑1 (MQ=60)
                                                                              CCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGCTTTTTTATGTTGTGTATT               <  SRR4302533.2390562/94‑1 (MQ=60)
                                                                              CCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGCTTTTTTATGTTGTGTATT               <  SRR4302533.3877014/94‑1 (MQ=60)
                                                                               CGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGCTTTTTTATGTTGTGTATTG              >  SRR4302533.1501829/1‑94 (MQ=60)
                                                                               CGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGCTTTTTTATGTTGTGTATTG              <  SRR4302533.4835556/94‑1 (MQ=60)
                                                                                GCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGCTTTTTTATGTTGTGTATTGT             <  SRR4302533.5294698/94‑1 (MQ=60)
                                                                                 CTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGCTTTTTTATGTTGTGTATTGTG            >  SRR4302533.2077926/1‑94 (MQ=60)
                                                                                  TGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGCTTTTTTATGTTGTGTATTGTGT           >  SRR4302533.4424013/1‑94 (MQ=60)
                                                                                   GTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGCTTTTTTATGTTGTGTATTGTGTT          <  SRR4302533.142213/94‑1 (MQ=60)
                                                                                   GTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGCTTTTTTATGTTGTGTATTGTGTT          >  SRR4302533.4187396/1‑94 (MQ=60)
                                                                                      GAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGCTTTTTTATGTTGTGTATTGTGTTGTG       <  SRR4302533.2132175/94‑1 (MQ=60)
                                                                                         AGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGCTTTTTTATGTTGTGTATTGTGTTGTGTGT    >  SRR4302533.4079561/1‑94 (MQ=60)
                                                                                           GCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGCTTTTTTATGTTGTGTATTGTGTTGTGTGTTT  >  SRR4302533.1432158/1‑94 (MQ=60)
                                                                                           GCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGCTTTTTTATGTTGTGTATTGTGTTGTGTGTTT  <  SRR4302533.857110/94‑1 (MQ=60)
                                                                                           |                                                                                             
CATCTTTCGATGAGAACGGTGCGTAGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGACCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGCTTTTTTATGTTGTGTATTGTGTTGTGTGTTT  >  NC_000913/2425682‑2425866

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑