Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1624	250.6	12620048	98.0%	12367647	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,557,200	T→C	V137A (GTG→GCG)	b2442 →	putative prophage integrase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,557,200	0	T	C	99.2%	754.3 / NA	238	V137A (GTG→GCG)	b2442	putative prophage integrase
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (126/110); minor base A (2/0); total (128/110)
Fisher's exact test for biased strand distribution p-value = 5.01e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 257 total aligned reads displayed.

CCCTTATTGCCAATGGAATTAACCCAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGTGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGTCGGAATCCTATGCAAAACAGGTCAGATCGGCACTAGAAAAAGACGTCTATC  >  NC_000913/2557110‑2557289

 ccTTATTGCCAATGGAATTAACCCAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAggcggc                                                                                        <  1:1506057/93‑1 (MQ=255)

  cTTATTGCCAATGGAATTAACCCAGTGGAACAAGCCCGCCAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGgcggcg                                                                                       >  2:1100144/1‑93 (MQ=255)

   ttATTGCCAATGGAATTAACCCAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGcggcgg                                                                                      >  1:3175234/1‑93 (MQ=255)

     aTTGCCAATGGAATTAACCCAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGCGGCGGaa                                                                                    <  2:4020986/93‑1 (MQ=255)

      ttGCCAATGGAATTAACCCAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGCGGCGGAAg                                                                                   <  2:4102639/93‑1 (MQ=255)

       tGCCAATGGAATTAACCCAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGCTGCGGAAGa                                                                                  <  2:1211776/93‑1 (MQ=255)

       tGCCAATGGAATTAACCCAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGCGGCGGAAGa                                                                                  >  2:1994152/1‑93 (MQ=255)

       tGCCAATGGAATTAACCCAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGCGGCGGAAGa                                                                                  <  2:5548947/93‑1 (MQ=255)

          cAATGGAATTAACCCAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGCGGCGGAAGACTg                                                                               <  1:87313/93‑1 (MQ=255)

           aaTGGAATTAACCCAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGCGGCGGAAGACTgg                                                                              >  2:6003541/1‑93 (MQ=255)

           aaTGGAATTAACCCAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGCGGCGGAAGACTgg                                                                              >  1:2525206/1‑93 (MQ=255)

            aTGGAATTAACCCAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGCGGCGGAAGACTGGt                                                                             <  2:1828146/93‑1 (MQ=255)

            aTGGAATTAACCCAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGCGGCGGAAGACTGGt                                                                             <  1:4938136/93‑1 (MQ=255)

             tGGAATTAACCCAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGCGGCGGAAGACTGGtt                                                                            >  1:4610109/1‑93 (MQ=255)

                 aTTAACCCAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGCGGCGGAAGACTGGTTTGCt                                                                        >  2:2392248/1‑93 (MQ=255)

                  ttAACCCAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGCGGCGGAAGACTGGTTTGCTa                                                                       <  2:4860538/93‑1 (MQ=255)

                   tAACCCAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGCGGCGGAAGACTGGTTTGCTAc                                                                      <  1:3439727/93‑1 (MQ=255)

                     aCCCAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGCGGCGGAAGACTGGTTTGCTACCa                                                                    >  2:2022106/1‑93 (MQ=255)

                      cccAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGCGGCGGAAGACTGGTTTGCTACCAg                                                                   <  1:5226388/93‑1 (MQ=255)

                        cAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGCGGCGGAAGACTGGTTTGCTACCAggg                                                                 >  2:5867305/1‑93 (MQ=255)

                        cAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGCGGCGGAAGACTGGTTTGCTACCAggg                                                                 >  1:38405/1‑93 (MQ=255)

                         aGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGCTTTAAGAAGGCGGCGGCAGACTGGTTTGCTACCAGGGt                                                                >  1:2989952/1‑93 (MQ=255)

                         aGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGCGGCGGAAGACTGGTTTGCTACCAGGGt                                                                >  1:5428296/1‑93 (MQ=255)

                         aGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGCGGCGGAAGACTGGTTTGCTACCAGGGt                                                                >  1:181992/1‑93 (MQ=255)

                          gTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGCGGCGGAAGACTGGTTTGCTACCAGGGtt                                                               >  2:4583176/1‑93 (MQ=255)

                            ggAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGCGGCGGAAGACTGGTTTGCTCTCAGGGCTgg                                                             >  2:3751194/1‑93 (MQ=255)

                            ggAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGCGGCGGAAGACTGGTTTGCTACCAGGGTTgg                                                             <  2:2143566/93‑1 (MQ=255)

                             gAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGCGGCGGAAGACTGGTTTGCTACCAGGGTTGGa                                                            <  2:5312593/93‑1 (MQ=255)

                              aaCAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGCGGCGGAAGACTGGTTTGCTACCAGGGTTGGAg                                                           <  1:1547555/93‑1 (MQ=255)

                               aCAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGCGGCGGAAGACTGGTTTGCTACCAGGGTTGGAgg                                                          >  2:431332/1‑93 (MQ=255)

                               aCAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGCGGCGGAAGACTGGTTTGCTACCAGGGTTGGAgg                                                          >  1:596116/1‑93 (MQ=255)

                               aCAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGCGGCGGAAGACTGGTTTGCTACCAGGGTTGGAgg                                                          >  1:1313112/1‑93 (MQ=255)

                                cAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGCGGCGGAAGACTGGTTTGCTACCAGGGTTGGAggg                                                         <  1:2424027/93‑1 (MQ=255)

                                 aaGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGCGGCGGATGACTGGTTTGCTACCAGGGTTGGAGggt                                                        <  2:5336481/93‑1 (MQ=255)

                                 aaGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGCGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGggt                                                        <  1:3618545/93‑1 (MQ=255)

                                   gCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGCGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGggtgg                                                      <  1:5130637/93‑1 (MQ=255)

                                     ccGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGCGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGTc                                                    >  1:3081842/1‑93 (MQ=255)

                                     ccGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGCGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGTc                                                    >  2:3807705/1‑93 (MQ=255)

                                      cGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGCGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGTCg                                                   >  2:127793/1‑93 (MQ=255)

                                      cGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGCGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGTCg                                                   >  2:5154810/1‑93 (MQ=255)

                                      cGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGCGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGGGGTCg                                                   >  1:3983160/1‑93 (MQ=255)

                                        cAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGCGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGTCGGa                                                 >  1:1017064/1‑93 (MQ=255)

                                         aaGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGCGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGTCGGaa                                                <  1:4568773/93‑1 (MQ=255)

                                         aaGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGCGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGTCGGaa                                                <  2:4644944/93‑1 (MQ=255)

                                          aGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGCGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGTCGGAAt                                               >  2:4077319/1‑93 (MQ=255)

                                          aGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGCGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGTCGGAAt                                               >  2:1630576/1‑93 (MQ=255)

                                          aGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGCGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGTCGGAAt                                               >  2:1618307/1‑93 (MQ=255)

                                           ggAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGCGGCGGAAGACTGGTTTGCTACCGGGGTTGGAGGGTGGTCGGAATc                                              >  1:2022187/1‑93 (MQ=255)

                                           ggAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGCGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGTCGGAATc                                              >  2:2614830/1‑93 (MQ=255)

                                           ggAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGCGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGTCGGAATc                                              >  1:2653143/1‑93 (MQ=255)

                                            gAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGCGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGTCGGAATcc                                             <  1:4880000/93‑1 (MQ=255)

                                              aaaaGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGCGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGTCGGAATCCTa                                           <  1:4233434/93‑1 (MQ=255)

                                              aaaaGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGCGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGTCGGAATCCTa                                           <  2:5457705/93‑1 (MQ=255)

                                               aaaGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGCGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGTCGGAATCCTAt                                          <  2:2913888/93‑1 (MQ=255)

                                               aaaGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGCGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGTCGGAATCCTAt                                          >  1:545145/1‑93 (MQ=255)

                                                 aGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGCGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGTCGGAATCCTATGc                                        >  1:1081781/1‑93 (MQ=255)

                                                     aTTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGCGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGTCGGAATCCTATGCaaaa                                    >  2:5099579/1‑93 (MQ=255)

                                                      ttGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGCGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGTCGGAATCCTATGCAAAAc                                   >  1:6081624/1‑93 (MQ=255)

                                                      ttGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGCGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGTCGGAATCCTATGCAAAAc                                   <  1:5303980/93‑1 (MQ=255)

                                                      ttGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGCGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGTCGGAATCCTATGCAAAAc                                   >  1:3783890/1‑93 (MQ=255)

                                                       tGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGCGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGTCGGAATCCTATGCAAAACa                                  <  2:4484895/93‑1 (MQ=255)

                                                        gATGCGCTCAACATGGCGGTAGGATTTAAGAAGGCGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGTCGGAATCCTATGCAAAACAg                                 >  2:6133269/1‑93 (MQ=255)

                                                         aTGCGCTCAACATGGCGGCAGGATTTAAGAAGGCGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGGCGGAATCCTATGCAAAACAgg                                >  2:4551410/1‑93 (MQ=255)

                                                          tGCGCTCAACATGGCGGCAGGATTTAAGAAGGCGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGTCGGAATCCTATGCAAAACAGGt                               <  1:1734730/93‑1 (MQ=255)

                                                            cgcTCAACATGGCGGCAGGATTTAAGAAGGCGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGTCGGAATCCTATGCAAAACATGTCa                             <  1:2370182/93‑1 (MQ=255)

                                                            cgcTCAACATGGCGGCAGGATTTAAGAAGGCGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGTCGGAATCCTATGCAAAACAGGTCa                             <  1:662658/93‑1 (MQ=255)

                                                            cgcTCAACATGGCGGCAGGATTTAAGAAGGCGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGTCGGAATCCTATGCAAAACAGGTCa                             <  1:4011667/93‑1 (MQ=255)

                                                            cgcTCAACATGGCGGCAGGATTTAAGAAGGCGGCGGAAGACTGGTTTGCTACCAGGGCTGGAGGGTGGTCGGAATCCTATGCAAAACAGGTCa                             >  2:2321509/1‑93 (MQ=255)

                                                             gcTCAACATGGCGGCAGGATTTAAGAAGGCGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGTCGGAATCCTATGCAAAACAGGTCAg                            >  1:134023/1‑93 (MQ=255)

                                                             gcTCAACATGGCGGCAGGATTTAAGAAGAAGGCGGGAGACTGGTTTGCTACCAGGGTTGGGGGGTGGGCGGCATCCTATGCAAAACAGGGCAg                            >  2:5619458/1‑93 (MQ=255)

                                                              cTCAACATGGCGGCAGGATTTAAGAAGGCGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGTCGGAATCCTATGCAAAACAGGTCAGa                           >  2:832404/1‑93 (MQ=255)

                                                              cTCAACATGGCGGCAGGATTTAAGAAGGCGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGTCGGAATCCTATGCAAAACAGGTCAGa                           >  2:3605642/1‑93 (MQ=255)

                                                               tCAACATGGCGGCAGGATTTAAGAAGGCGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGTCGGAATCCTATGCAAAACAGGTCAGAt                          <  2:38405/93‑1 (MQ=255)

                                                                cAACATGGCGGCAGGATTTAAGAAGGCGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGTCGGAATCCTATGCAAAACAGGTCAGATc                         <  1:2540576/93‑1 (MQ=255)

                                                                 aaCATGGCGGCAGGATTTAAGAAGGCGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGTCGGAATCCTATGCAAAACAGGTCAGATCg                        >  1:5560423/1‑93 (MQ=255)

                                                                  aCATGGCGGCAGGATTTAAGAAGGCGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGTCGGAATCCTATGCAAAACAGGTCAGATCgg                       >  1:2292592/1‑93 (MQ=255)

                                                                  aCATGGCGGCAGGATTTAAGAAGGCGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGTCGGAATCCTATGCAAAACAGGTCAGATCgg                       >  2:2473711/1‑93 (MQ=255)

                                                                   cATGGCGGCAGGATTTAAGAAGGCGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGTCGGAATCCTGTGCAAAACAGGTCAGATCGGc                      <  2:4008629/93‑1 (MQ=255)

                                                                   cATGGCGGCAGGATTTAAGAAGGCGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGTCGGAATCCTATGCAAAACAGGTCAGATCGGc                      <  2:5047343/93‑1 (MQ=255)

                                                                   cATGGCGGCAGGATTTAAGAAGGCGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGTCGGAATCCTATGCAAAACAGGTCAGATCGGc                      >  2:1454623/1‑93 (MQ=255)

                                                                      ggcggcAGGATTTAAGAAGGCGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGTCGGAATCCTATGCAAAACAGGTCAGATCGGCACt                   >  1:4366455/1‑93 (MQ=255)

                                                                        cggcAGGATTTAAGAAGGCGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGTCGGAATCCTATGCAAAACAGGTCAGATCGGCACTAg                 >  2:1044267/1‑93 (MQ=255)

                                                                         ggcAGGATTTAAGAAGGCGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGTCGGAATCCTATGCAAAACAGGTCAGATCGGCACTAGa                <  2:334996/93‑1 (MQ=255)

                                                                         ggcAGGATTTAAGAAGGCGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGTCGGAATCCTATGCAAAACAGGTCAGATCGGCACTAGa                <  1:5723803/93‑1 (MQ=255)

                                                                          gcAGGATTTAAGAAGGCGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGTCGGAATCCTATGCAAAACAGGTCAGATCGGCACTAGaa               >  1:5488541/1‑93 (MQ=255)

                                                                          gcAGGATTTAAGAAGGCGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGTCGGAATCCTATGCAAAACAGGTCAGATCGGCACTAGaa               <  1:6059287/93‑1 (MQ=255)

                                                                          gcAGGATTTAAGAAGGCGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGTCGGAATCCTATGCAAAACAGGTCAGATCGGCACTAGaa               >  2:1511893/1‑93 (MQ=255)

                                                                           cAGGATTTAAGAAGGCGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGTCGGAATCCTATGCAAAACAGGTCAGATCGGCACTAGaaa              >  2:3296251/1‑93 (MQ=255)

                                                                             ggATTTAAGACGGCGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGTCGGAATCCTATGCAAAACAGGTCAGATCGGCACTAGaacaa            >  1:2224554/1‑93 (MQ=255)

                                                                             ggATTTAAGAAGGCGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGCGGTCCGAATCCTATGCAAAACAGGTCAGATCGGCACTAGaaaaa            >  1:5823773/1‑93 (MQ=255)

                                                                               aTTTAAGAAGGCGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGTGGGAATCCTATGCAAAACAGGTCAGATCGGCACTAGAAAAAGa          <  1:4443098/93‑1 (MQ=255)

                                                                               aTTTAAGAAGGCGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGTCGGAATCCTATGCAAAACAGGTCAGATCGGCACTAGAAAAAGa          <  2:3384080/93‑1 (MQ=255)

                                                                                tttAAGAAGGCGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGTCGGAATCCTATGCAAAACAGGTCAGATCGGCACTAGAAAAAGAc         <  1:274304/93‑1 (MQ=255)

                                                                                  tAAGAAGGCGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGTCGGAATCCTATGCAAAACAGGTCAGATCGGCACTAGAAAAAGACGt       >  2:1334566/1‑93 (MQ=255)

                                                                                   aagaagGCGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGTCGGAATCCTATGCAAAACAGGTCAGATCGGCACTAGAAAAAGACGTc      >  1:3482855/1‑93 (MQ=255)

                                                                                    agaagGCGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGTCGGAATCCTATGCAAAACAGGTCAGATCGGCACTAGAAAAAGACGTCt     <  2:4370748/93‑1 (MQ=255)

                                                                                    agaagGCGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGTCGGAATCCTATGCAAAACAGGTCAGATCGGCACTAGAAAAAGACGTCt     <  2:5894421/93‑1 (MQ=255)

                                                                                      aagGCGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGTCGGAATCCTATGCAAAACAGGTCAGATCGGCACTAGAAAAAGACGTCTAt   >  2:4149036/1‑93 (MQ=255)

                                                                                       agGCGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGTCGGAATCCTATGCAAAACAGGTCAGATCGGCACTAGAAAAAGACGTCTATc  >  2:51184/1‑93 (MQ=255)

                                                                                       agGCGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGTCGGAATCCTATGCAAAACAGGTCAGATCGGCACTAGAAAAAGACGTCTATc  <  2:3241625/93‑1 (MQ=255)

CCCTTATTGCCAATGGAATTAACCCAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGTGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGTCGGAATCCTATGCAAAACAGGTCAGATCGGCACTAGAAAAAGACGTCTATC  >  NC_000913/2557110‑2557289

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

GATCCCTTATTGCCAATGGAATTAACCCAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGTGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGTCGGAATCCTATGCAAAACAGGTCAGATCGGCACTAGAAAAAGACGTCTATCCGGT  >  NC_000913/2557107‑2557293

GATCCCTTATTGCCAATGGAATTAACCCAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGC                                                                                               <  SRR4302533.4503923/94‑1 (MQ=60)

GATCCCTTATTGCCAATGGAATTAACCCAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGC                                                                                               <  SRR4302533.5838807/94‑1 (MQ=60)

GATCCCTTATTGCCAATGGAATTAACCCAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGC                                                                                               <  SRR4302533.5200666/94‑1 (MQ=60)

GATCCCTTATTGCCAATGGAATTAACCCAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGC                                                                                               >  SRR4302533.5213884/1‑94 (MQ=60)

  TCCCTTATTGCCAATGGAATTAACCCAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGCGG                                                                                             >  SRR4302533.5517038/1‑94 (MQ=60)

   CCCTTATTGCCAATGGAATTAACCCAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGCGGC                                                                                            >  SRR4302533.2757389/1‑94 (MQ=60)

    CCTTATTGCCAATGGAATTAACCCAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGCGGCG                                                                                           >  SRR4302533.3493576/1‑94 (MQ=60)

    CCTTATTGCCAATGGAATTAACCCAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGCGGCG                                                                                           <  SRR4302533.1515138/94‑1 (MQ=60)

    CCTTATTGCACATGGAATTAACCCAGTGGCACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGCGGCG                                                                                           <  SRR4302533.2122474/94‑1 (MQ=60)

     CTTATTGCCAATGGAATTAACCCAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGCGGCGG                                                                                          >  SRR4302533.3194769/1‑94 (MQ=60)

     CTTATTGCCAATGGAATTAACCCAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGCGGCGG                                                                                          >  SRR4302533.6004662/1‑94 (MQ=60)

      TTATTGCCAATGGAATTAACCCAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGCGGCGGA                                                                                         <  SRR4302533.9299/94‑1 (MQ=60)

        ATTGCCAATGGAATTAACCCAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGCGGCGGAAG                                                                                       <  SRR4302533.5276798/94‑1 (MQ=60)

           GCCAATGGAATTAACCCAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGCGGCGGAAGACT                                                                                    >  SRR4302533.5338391/1‑94 (MQ=60)

             CAATGGAATTAACCCAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGCGGCGGAAGACTGG                                                                                  <  SRR4302533.87872/94‑1 (MQ=60)

             CAATGGAATTAACCCAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGCGGCGGAAGACTGG                                                                                  >  SRR4302533.1525749/1‑94 (MQ=60)

             CAATGGAATTAACCCAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGCGGCGGAAGACTGG                                                                                  >  SRR4302533.2540756/1‑94 (MQ=60)

               ATGGAATTAACCCAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGCGGCGGAAGACTGGTT                                                                                <  SRR4302533.4397797/94‑1 (MQ=60)

               ATGGAATTAACCCAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGCGGCGGAAGACTGGTT                                                                                <  SRR4302533.4968031/94‑1 (MQ=60)

               ATGGAATTAACCCAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGCGGCGGAAGACTGGTT                                                                                >  SRR4302533.4638028/1‑94 (MQ=60)

                TGGAATTAACCCAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGCGGCGGAAGACTGGTTT                                                                               <  SRR4302533.5471210/94‑1 (MQ=60)

                    ATTAACCCAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGCGGCGGAAGACTGGTTTGCTA                                                                           <  SRR4302533.3034504/94‑1 (MQ=60)

                     TTAACCCAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGCGGCGGAAGACTGGTTTGCTAC                                                                          <  SRR4302533.2579613/94‑1 (MQ=60)

                      TAACCCAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGCGGCGGAAGACTGGTTTGCTACC                                                                         <  SRR4302533.3460971/94‑1 (MQ=60)

                       AACCCAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGCGGCGGAAGACTGGTTTGCTACCA                                                                        >  SRR4302533.5702714/1‑94 (MQ=60)

                        ACCCAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGCGGCGGAAGACTGGTTTGCTACCAG                                                                       <  SRR4302533.486660/94‑1 (MQ=60)

                         CCCAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGCGGCGGAAGACTGGTTTGCTACCAGG                                                                      <  SRR4302533.5257837/94‑1 (MQ=60)

                          CCAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGCGGCGGAAGACTGGTTTGCTACCAGGG                                                                     <  SRR4302533.4150896/94‑1 (MQ=60)

                          CCAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGCGGCGGAAGACTGGTTTGCTACCAGGG                                                                     >  SRR4302533.38657/1‑94 (MQ=60)

                           CAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGCTTTAAGAAGGCGGCGGCAGACTGGTTTGCTACCAGGGT                                                                    >  SRR4302533.3008239/1‑94 (MQ=60)

                           CAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGCGGCGGAAGACTGGTTTGCTACCAGGGT                                                                    >  SRR4302533.5966217/1‑94 (MQ=60)

                           CAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGCGGCGGAAGACTGGTTTGCTACCAGGGT                                                                    >  SRR4302533.5460988/1‑94 (MQ=60)

                           CAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGCGGCGGAAGACTGGTTTGCTACCAGGGT                                                                    >  SRR4302533.183123/1‑94 (MQ=60)

                            AGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGCGGCGGAAGACTGGTTTGCTACCAGGGTT                                                                   >  SRR4302533.5140604/1‑94 (MQ=60)

                            AGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGCGGCGGAAGACTGGTTTGCTACCAGGGTT                                                                   >  SRR4302533.29650/1‑94 (MQ=60)

                            AGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGCGGCGGAAGACTGGTTTGCTACCAGGGGT                                                                   >  SRR4302533.2251557/1‑94 (MQ=60)

                             ATGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGCGGCGGAAGACTGGTTTGCTACCAGGGTTG                                                                  <  SRR4302533.625615/94‑1 (MQ=60)

                              TGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGCGGCGGAAGACTGGTTTGCTACCAGGGTTGG                                                                 <  SRR4302533.4030808/94‑1 (MQ=60)

                              TGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGCGGCGGAAGACTGGTTTGCTACCAGGGTTGG                                                                 >  SRR4302533.3960439/1‑94 (MQ=60)

                                GAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGCGGCGGAAGACTGGTTTGCTACCAGGGTTGGAG                                                               <  SRR4302533.2134011/94‑1 (MQ=60)

                                 AACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGCGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGG                                                              >  SRR4302533.4571519/1‑94 (MQ=60)

                                 AACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGCGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGG                                                              >  SRR4302533.599631/1‑94 (MQ=60)

                                 AACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGCGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGG                                                              >  SRR4302533.1320992/1‑94 (MQ=60)

                                 AACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGCGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGG                                                              >  SRR4302533.4922762/1‑94 (MQ=60)

                                 AACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGCGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGG                                                              <  SRR4302533.1556880/94‑1 (MQ=60)

                                 AACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGCGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGG                                                              <  SRR4302533.3436666/94‑1 (MQ=60)

                                  ACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGCGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGG                                                             >  SRR4302533.1430018/1‑94 (MQ=60)

                                   CAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGCGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGT                                                            <  SRR4302533.2438991/94‑1 (MQ=60)

                                   CAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGCGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGT                                                            <  SRR4302533.1443856/94‑1 (MQ=60)

                                    AAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGCGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTG                                                           <  SRR4302533.3640802/94‑1 (MQ=60)

                                      GCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGCGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGT                                                         <  SRR4302533.5161630/94‑1 (MQ=60)

                                       CCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGCGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGTC                                                        >  SRR4302533.3100749/1‑94 (MQ=60)

                                       CCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGCGGCGGAAGACTGGCTTGCTACCAGGGGTGGAGGGTGGTC                                                        >  SRR4302533.868091/1‑94 (MQ=60)

                                        CCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGCGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGTCG                                                       <  SRR4302533.3454516/94‑1 (MQ=60)

                                        CCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGCGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGTCG                                                       >  SRR4302533.2342829/1‑94 (MQ=60)

                                        CCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGCGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGTCG                                                       >  SRR4302533.2391151/1‑94 (MQ=60)

                                        CCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGCGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGGGGTCG                                                       >  SRR4302533.4007408/1‑94 (MQ=60)

                                          GCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGCGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGTCGGA                                                     >  SRR4302533.1023042/1‑94 (MQ=60)

                                           CAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGATGGCGGCGGCAGACTGGTTTGCTAGCAGGGGTGGAGGGTGGTCGGAA                                                    >  SRR4302533.5940155/1‑94 (MQ=60)

                                            AAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGCGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGTCGGAAT                                                   <  SRR4302533.4596464/94‑1 (MQ=60)

                                            AAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGCGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGTCGGAAT                                                   >  SRR4302533.4173604/1‑94 (MQ=60)

                                            AAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGCGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGTCGGAAT                                                   <  SRR4302533.2680751/94‑1 (MQ=60)

                                             AGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGCGGCGGAAGACTGGTTTGCTACCGGGGTTGGAGGGTGGTCGGAATC                                                  >  SRR4302533.2034642/1‑94 (MQ=60)

                                             AGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGCGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGTCGGAATC                                                  >  SRR4302533.2669441/1‑94 (MQ=60)

                                               GAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGCGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGTCGGAATCCT                                                <  SRR4302533.4909566/94‑1 (MQ=60)

                                               GAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGCGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGTCGGAATCCT                                                <  SRR4302533.6223504/94‑1 (MQ=60)

                                                AAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGCGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGTCGGAATCCTA                                               <  SRR4302533.1328234/94‑1 (MQ=60)

                                                 AAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGCGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGTCGGAATCCTAT                                              <  SRR4302533.4259016/94‑1 (MQ=60)

                                                 AAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGCGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGTCGGAATCCTAT                                              >  SRR4302533.548383/1‑94 (MQ=60)

                                                  AAAGCCATTGATGCGCTCAACATGGCGGCAGGCTTTAAGAAGGCGGCGGAAGACTGGCTTGCTAACAGGGTTGGCGGGTGGTCGGAATCCTAAG                                             >  SRR4302533.2022020/1‑94 (MQ=60)

                                                   AAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGCGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGTCGGAATCCTATGC                                            >  SRR4302533.1557231/1‑94 (MQ=60)

                                                   AAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGCGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGTCGGAATCCTATGC                                            >  SRR4302533.1088163/1‑94 (MQ=60)

                                                    AGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGCGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGTCGGAATCCTATGCA                                           >  SRR4302533.2030985/1‑94 (MQ=60)

                                                    AGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGCGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGTCGGAATCCTATGCA                                           >  SRR4302533.4670162/1‑94 (MQ=60)

                                                    AGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGCGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGTCGGAATCCTATGCA                                           >  SRR4302533.3718530/1‑94 (MQ=60)

                                                     GCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGCGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGTCGGAATCCTATGCAA                                          >  SRR4302533.4603065/1‑94 (MQ=60)

                                                       CATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGCGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGTCGGAATCCTATGCAAAA                                        >  SRR4302533.5058981/1‑94 (MQ=60)

                                                       CATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGCGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGTCGGAATCCTATGCAAAA                                        >  SRR4302533.3684757/1‑94 (MQ=60)

                                                        ATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGCGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGTCGGAATCCTATGCAAAAC                                       >  SRR4302533.3899363/1‑94 (MQ=60)

                                                        ATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGCGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGTCGGAATCCTATGCAAAAC                                       >  SRR4302533.6118401/1‑94 (MQ=60)

                                                        ATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGCGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGTCGGAATCCTATGCAAAAC                                       >  SRR4302533.3807060/1‑94 (MQ=60)

                                                         TTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGCGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGTCGGAATCCTATGCAAAACA                                      <  SRR4302533.5335758/94‑1 (MQ=60)

                                                          TGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGCGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGCGGTCGGAATCCTATGCAAAACAG                                     >  SRR4302533.5489461/1‑94 (MQ=60)

                                                             TGCGCTCAACATGGCGGCAGGATTTAAGAAGGCGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGTCGGAATCCTATGCAAAACAGGTC                                  <  SRR4302533.1745255/94‑1 (MQ=60)

                                                              GCGCTCAACATGGCGGCAGGATTTAAGAAGGCGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGTCGGAATCCTATGCAAAACAGGTCA                                 <  SRR4302533.5205420/94‑1 (MQ=60)

                                                               CGCTCAACATGGCGGCAGGATTTAAGAAGGCGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGTCGGAATCCTATGCAAAACATGTCAG                                <  SRR4302533.2384842/94‑1 (MQ=60)

                                                               CGCTCAACATGGCGGCAGGATTTAAGAAGGCGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGTCGGAATCCTATGCAAAACAGGTCAG                                >  SRR4302533.134871/1‑94 (MQ=60)

                                                               CGCTCAACATGGCGGCAGGATTTAAGAAGGCGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGTCGGAATCCTATGCAAAACAGGTCAG                                <  SRR4302533.666571/94‑1 (MQ=60)

                                                               CGCTCAACATGGCGGCAGGATTTAAGAAGGCGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGTCGGAATCCTATGCAAAACAGGTCAG                                <  SRR4302533.4036093/94‑1 (MQ=60)

                                                                GCTCAACATGGCGGCAGGATTTAAGAAGGCGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGTCGGAATCCTATGCAAAACAGGTCAGA                               <  SRR4302533.1055526/94‑1 (MQ=60)

                                                                GCTCAACATGGCGGCAGGATTTAAGAAGGCGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGTCGGAATCCTATGCAAAACAGGTCAGA                               >  SRR4302533.3855036/1‑94 (MQ=60)

                                                                 CTCAACATGGCGGCAGGATTTAAGAAGGCGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGTCGGAATCCTATGCAAAACAGGTCAGAT                              >  SRR4302533.5321513/1‑94 (MQ=60)

                                                                  TCAACATGGCGGCAGGATTTAAGAAGGCGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGTCGGAATCCTATGCAAAACAGGTCAGATC                             >  SRR4302533.4338338/1‑94 (MQ=60)

                                                                   CAACATGGCGGCAGGATTTAAGAAGGCGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGTCGGAATCCTATGCAAAACAGGTCAGATCG                            >  SRR4302533.6085642/1‑94 (MQ=60)

                                                                   CAACATGGCGGCAGGATTTAAGAAGGCGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGTCGGAATCCTATGCAAAACAGGTCAGATCG                            <  SRR4302533.2556215/94‑1 (MQ=60)

                                                                   CAACATGGCGGCAGGATTTAAGAAGGCGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGTCGGAATCCTATGCAAAACAGGTCAGATCG                            >  SRR4302533.5365770/1‑94 (MQ=60)

                                                                   CAACATGGCGGCAGGATTTAAGAAGGCGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGTCGGAATCCTATGCAAAACAGGTCAGATCG                            >  SRR4302533.5594027/1‑94 (MQ=60)

                                                                    AACATGGCGGCAGGATTTAAGAAGGCGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGTCGGAATCCTATGCAAAACAGGTCAGATCGG                           >  SRR4302533.2306786/1‑94 (MQ=60)

                                                                    AACATGGCGGCAGGATTTAAGAAGGCGGCGGAAGACTGGTTTGATACCAGGGTTGGAGGGTGGTCGGAATCCTATGCAAAACAGGTCAGATCGG                           >  SRR4302533.4461439/1‑94 (MQ=60)

                                                                      CATGGCGGCAGGATTTAAGAAGGCGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGTCGGAATCCTATGCAAAACAGGTCAGATCGGCA                         <  SRR4302533.3607000/94‑1 (MQ=60)

                                                                       ATGGCGGCAGGATTTAAGAAGGCGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGTCGGAATCCTATGCAAAACAGGTCAGATCGGCAC                        >  SRR4302533.255164/1‑94 (MQ=60)

                                                                        TGGCGGCAGGATTTAAGAAGGCGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGTCGGAATCCTATGCAAAACAGGTCAGATCGGCACT                       >  SRR4302533.4392812/1‑94 (MQ=60)

                                                                        TGGCGGCAGGATTTAAGAAGGCGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGTCGGAATCCTATGCAAAACAGGTCAGATCGGCACT                       <  SRR4302533.5570029/94‑1 (MQ=60)

                                                                           CGGCAGGATTTAAGAAGGCGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGTCGGAATCCTATGCAAAACAGGTCAGATCGGCACTAGA                    >  SRR4302533.2377808/1‑94 (MQ=60)

                                                                            GGCAGGATTTAAGAAGGCGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGTCGGAATCCTATGCAAAACAGGTCAGATCGGCACTAGAA                   >  SRR4302533.5521629/1‑94 (MQ=60)

                                                                            GGCAGGATTTAAGAAGGCGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGTCGGAATCCTATGCAAAACAGGTCAGATCGGCACTAGAA                   <  SRR4302533.5758361/94‑1 (MQ=60)

                                                                            GGCAGGATTTAAGAAGGCGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGTCGGAATCCTATGCAAAACAGGTCAGATCGGCACTAGAA                   >  SRR4302533.4473725/1‑94 (MQ=60)

                                                                            GGCAGGATTTAAGAAGGCGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGTCGGAATCCTATGCAAAACAGGTCAGATCGGCACTAGAA                   >  SRR4302533.6102992/1‑94 (MQ=60)

                                                                             GCAGGATTTAAGAAGGCGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGTCGGAATCCTATGCAAAACAGGTCAGATCGGCACTAGAAA                  <  SRR4302533.6095969/94‑1 (MQ=60)

                                                                               AGGATTTAAGACGGCGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGTCGGAATCCTATGCAAAACAGGTCAGATCGGCACTAGAACAA                >  SRR4302533.2238290/1‑94 (MQ=60)

                                                                               AGGATTTAAGAAGGCGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGTCGGAATCCTATGCAAAACAGGTCAGATCGGCACTAGAAAAA                <  SRR4302533.3537887/94‑1 (MQ=60)

                                                                               AGGATTTAAGAAGGCGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGCGGTCCGAATCCTATGCAAAACAGGTCAGATCGGCACTAGAAAAA                >  SRR4302533.5858950/1‑94 (MQ=60)

                                                                                GGATTTAAGAAGGCGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGTCGGAATCCTATGCAAAACAGGTCAGATCGGCACTAGAAAAAG               <  SRR4302533.2459259/94‑1 (MQ=60)

                                                                                  ATTTAAGAAGGCGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGTGGGAATCCTATGCAAAACAGGTCAGATCGGCACTAGAAAAAGAC             <  SRR4302533.4469973/94‑1 (MQ=60)

                                                                                  ATTTAAGAAGGCGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGTCGGAATCCTATGCAAAACAGGTCAGATCGGCACTAGAAAAAGAC             <  SRR4302533.3127545/94‑1 (MQ=60)

                                                                                   TTTAAGAAGGCGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGTCGGAATCCTATGCCAAACAGGTCAGATCGGCACTAGAAAAAGACG            >  SRR4302533.2492541/1‑94 (MQ=60)

                                                                                   TTTAAGAAGGCGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGTCGGAATCCTATGCAAAACAGGTCAGATCGGCACTAGAAAAAGACG            <  SRR4302533.275973/94‑1 (MQ=60)

                                                                                   TTTAAGAAGGCGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGTCGGAATCCTATGCAAAACAGGTCAGATCGGCACTAGAAAAAGACG            <  SRR4302533.1629934/94‑1 (MQ=60)

                                                                                    TTAAGAAGGCGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGTCGGAATCCTATGCAAAACAGGTCAGATCGGCACTAGAAAAAGACGT           <  SRR4302533.1820293/94‑1 (MQ=60)

                                                                                     TAAGAAGGCGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGTCGGAATCCTATGCAAAACAGGTCAGATCGGCACTGGAAAAAGACGTC          <  SRR4302533.5831230/94‑1 (MQ=60)

                                                                                     TAAGAAGGCGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGTCGGAATCCTATGCAAAACAGGTCAGATCGGCACTAGAAAAAGACGTC          >  SRR4302533.3504340/1‑94 (MQ=60)

                                                                                     TAAGAAGGCGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGTCGGAATCCTATGCAAAACAGGTCAGATCGGCACTAGAAAAAGACGTC          <  SRR4302533.5604162/94‑1 (MQ=60)

                                                                                      AAGAAGGCGGCGGAAGACTGGTGTGCTACCAGGGTTGGAGGGTGGTCGGAATCCTATGCAAAACAGGTCAGATCGGCACTAGAAAAAGACGTCT         <  SRR4302533.1171170/94‑1 (MQ=60)

                                                                                       AGAAGGCGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGTCGGAATCCTATGCAAAACAGGTCAGATCGGCACTAGAAAAAGACGTCTA        <  SRR4302533.3013443/94‑1 (MQ=60)

                                                                                        GAAGGCGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGTCGGAATCCTATGCAAAACAGGTCAGATCGGCACTAGAAAAAGACGTCTAT       <  SRR4302533.2656398/94‑1 (MQ=60)

                                                                                          AGGCGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGTCGGAATCCTATGCAAAACAGGTCAGATCGGCACTAGAAAAAGACGTCTATCC     <  SRR4302533.1234654/94‑1 (MQ=60)

                                                                                          AGGCGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGTCGGAATCCTATGCAAAACAGGTCAGATCGGCACTAGAAAAAGACGTCTATCC     >  SRR4302533.3202874/1‑94 (MQ=60)

                                                                                             CGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGTCGGAATCCTATGCAAAACAGGTCAGATCGGCACTAGAAAAAGACGTCTATCtgga  >  SRR4302533.5147032/1‑90 (MQ=60)

GATCCCTTATTGCCAATGGAATTAACCCAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGTGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGTCGGAATCCTATGCAAAACAGGTCAGATCGGCACTAGAAAAAGACGTCTATCCGGT  >  NC_000913/2557107‑2557293

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 8 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`