Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1624	250.6	12620048	98.0%	12367647	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,583,799	(T)_7→6	coding (49/1701 nt)	narQ →	NarQ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,583,793	0	T	.	100.0%	993.2 / NA	215	coding (43/1701 nt)	narQ	NarQ
Reads supporting (aligned to +/- strand): ref base T (0/0); new base . (110/105); total (110/105)

Only 100 of 234 total aligned reads displayed.

TGCGTTTCAGCGAACTGGAACATTAATGATTTTTTGTGGAGAAGACGCGTGTGATTGTTAAACGACCCGTCTCGGCCAGTCTGGCCCGGGCCTTTTTTTACATTGTGCTGCTGTCGATTCTTTCCACGGGTATCGCTCTGCTAACTCTGGCGAGCAGTTTGCGCGACGCTGAGGCTATCAA  >  NC_000913/2583701‑2583881

 gCGTTTCAGCGAACTGGAACATTAATGATTTTTTGTGGAGAAGACGCGTGTGATTGTTAAACGACCCGTCTCGGCCAGTCTGGCCCGGGCCtt                                                                                         <  1:2231520/93‑1 (MQ=255)

 gCGTTTCAGCGAACTGGAACATTAATGATTTTTTGTGGAGAAGACGCGTGTGATTGTTAAACGACCCGTCTCGGCCAGTCTGGCCCGGGCCtt                                                                                         >  2:2448559/1‑93 (MQ=255)

  cGTTTCAGCGAACTGGAACATTAATGATTTTTTGTGGAGAAGACGCGTGTGATTGTTAAACGACCCGTCTCGGCCAGTCTGGCCCGGGCCttt                                                                                        <  1:272966/93‑1 (MQ=255)

  cGTTTCAGCGAACTGGAACATTAATGATTTTTTGTGGAGAAGACGCGTGTGATTGTTAAACGACCCGTCTCGGCCAGTCTGGCCCGGGCCttt                                                                                        >  1:3407803/1‑93 (MQ=255)

    tttCAGCGAACTGGAACATTAATGATTTTTTGTGGAGAAGACGCGTGTGATTGTTAAACGACCCGTCTCGGCCAGTCTGGCCCGGGCCttttt                                                                                      >  2:3486199/1‑93 (MQ=255)

    tttCAGCGAACTGGAACATTAATGATTTTTTGTGGAGAAGACGCGTGTGATTGTTAAACGACCCGTCTCGGCCAGTCTGGCCCGGGCCttttt                                                                                      <  2:5113937/93‑1 (MQ=255)

    tttCAGCGAACTGGAACATTAATGATTTTTTGTGGAGAAGACGCGTGTGATTGTTAAACGACCCGTCTCGGCCAGTCTGGCCCGGGCCttttt                                                                                      >  2:3979544/1‑93 (MQ=255)

          cGAACTGGAACATTAATGATTTTTTGTGGAGAAGACGCGTGTGATTGTTAAACGACCCGTCTCGGCCAGTCTGGCCCGGGCC‑TTTTTTACAtt                                                                               <  1:548213/93‑1 (MQ=255)

            aaCTGGAACATTAATGATTTTTTGTGGAGAAGACGCGTGTGATTGTTAAACGACCCGTCTCGGCCAGTCTGGCCCGGGCC‑TTTTTTACATtgt                                                                             >  1:306947/1‑93 (MQ=255)

            aaCTGGAACATTAATGATTTTTTGTGGAGAAGACGCGTGTGATTGTTAAACGACCCGTCTCGGCCAGTCTGGCCCGGGCC‑TTTTTTACATtgt                                                                             >  1:4467603/1‑93 (MQ=255)

             aCTGGAACATTAATGATTTTTTGTGGAGAAGACGCGTGTGATTGTTAAACGACCCGTCTCGGCCAGTCTGGCCCGGGCC‑TTTTTTACATtgtg                                                                            <  2:3014779/93‑1 (MQ=255)

             aCTGGAACATTAATGATTTTTTGTGGAGAAGACGCGTGTGATTGTTAAACGACCCGTCTCGGCCAGTCTGGCCCGGGCC‑TTTTTTACATtgtg                                                                            >  1:3654469/1‑93 (MQ=255)

              cTGGAACATTAATGATTTTTTGTGGAGAAGACGCGTGTGATTGTTAAACGACCCGTCTCGGCCAGTCTGGCCCGGGCC‑TTTTTTACATTGtgc                                                                           >  2:4260895/1‑93 (MQ=255)

              cTGGAACATTAATGATTTTTTGTGGAGAAGACGCGTGTGATTGTTAAACGACCCGTCTCGGCCAGTCTGGCCCGGGCC‑TTTTTTACATTGtgc                                                                           >  1:165587/1‑93 (MQ=255)

              cTGGAACATTAATGATTTTTTGTGGAGAAGACGCGTGTGATTGTTAAACGACCCGTCTCGGCCAGTCTGGCCCGGGCC‑TTTTTTACATTGtgc                                                                           >  2:5697481/1‑93 (MQ=255)

               tGGAACATTAATGATTTTTTGTGGAGAAGACGCGTGTGATTGTTAAACGACCCGTCTCGGCCAGTCTGGCCCGGGCC‑TTTTTTACATTGtgct                                                                          <  2:51828/93‑1 (MQ=255)

               tGGAACATTAATGATTTTTTGTGGAGAAGACGCGTGTGATTGTTAAACGACCCGTCTCGGCCAGTCTGGCCCGGGCC‑TTTTTTACATTGtgct                                                                          >  2:1878092/1‑93 (MQ=255)

               tGGAACATTAATGATTTTTTGTGGAGAAGACGCGTGTGATTGTTAAACGACCCGTCTCGGCCAGTCTGGCCCGGGCC‑TTTTTTACATTGtgct                                                                          >  1:6025953/1‑93 (MQ=255)

                  aaCATTAATGATTTTTTGTGGAGAAGACGCGTGTGATTGTTAAACGACCCGTCTCGGCCAGTCTGGCCCGGGCC‑TTTTTTACATTGTgctgct                                                                       >  2:3762388/1‑93 (MQ=255)

                  aaCATTAATGATTTTTTGTGGAGAAGACGCGTGTGATTGTTAAACGACCCGTCTCGGCCAGTCTGGCCCGGGCC‑TTTTTTACATTGTgctgct                                                                       <  2:2363632/93‑1 (MQ=255)

                    cATTAATGATTTTTTGTGGAGAAGACGCGTGTGATTGTTAAACGACCCGTCTCGGCCAGTCTGGCCCGGGCC‑TTTTTTACATTGTGCTGCTGt                                                                     >  1:5631283/1‑93 (MQ=255)

                     aTTAATGATTTTTTGTGGAGAAGACGCGTGTGATTGTTAAACGACCCGTCTCGGCCAGTCTGGCCCGGGCC‑TTTTTTACATTGTGCTGCTGTc                                                                    <  1:1849632/93‑1 (MQ=255)

                       tAATGATTTTTTGTGGAGAAGACGCGTGTGATTGTTAAACGACCCGTCTCGGCCAGTCTGGCCCGGGCC‑TTTTTTACATTGTGCTGCTGTCGa                                                                  >  2:2070145/1‑93 (MQ=255)

                       tAATGATTTTTTGTGGAGAAGACGCGTGTGATTGTTAAACGACCCGTCTCGGCCAGTCTGGCCCGGGCC‑TTTTTTACATTGTGCTGCTGTCGa                                                                  >  1:4932880/1‑93 (MQ=255)

                         aTGATTTTTTGTGGAGAAGACGCGTGTGATTGTTAAACGACCCGTCTCGGCCAGTCTGGCCCGGGCC‑TTTTTTACATTGTGCTGCTGTCGAtt                                                                <  1:3012915/93‑1 (MQ=255)

                          tGATTTTTTGTGGAGAAGACGCGTGTGATTGTTAAACGACCCGTCTGGGCCAGTCTGACCCGGGCC‑TTTTTTACATTGTGCTGCTGTCGATTc                                                               >  2:2145305/1‑93 (MQ=255)

                          tGATTTTTTGTGGAGAAGACGCGTGTGATTGTTAAACGACCCGTCTCGGCCAGTCTGGCCCGGGCC‑TTTTTTACATTGTGCTGCTGTCGATTc                                                               <  2:4485426/93‑1 (MQ=255)

                           gATTTTTTGTGGAGAAGACGCGTGTGATTGTTAAACGACCCGTCTCGGCCAGTCTGGCCCGGGCC‑TTTTTTACATTGTGCTGCTGTCGATTCt                                                              <  2:5915658/93‑1 (MQ=255)

                           gATTTTTTGTGGAGAAGACGCGTGTGATTGTTAAACGACCCGTCTCGGCCAGTCTGGCCCGGGCC‑TTTTTTACATTGTGCTGCTGTCGATTCt                                                              >  1:438948/1‑93 (MQ=255)

                           gATTTTTTGTGGAGAAGACGCGTGTGATTGTTAAACGACCCGTCTCGGCCAGTCTGGCCCGGGCC‑TTTTTTACATTGTGCTGCTGTCGATTCt                                                              >  1:6152831/1‑93 (MQ=255)

                           gATTTTTTGTGGAGAAGACGCGTGTGATTGTTAAACGACCCGTCTCGGCCAGTCTGGCCCGGGCC‑TTTTTTACATTGTGCTGCTGTCGATTCt                                                              <  1:2401482/93‑1 (MQ=255)

                             ttttttGTGGAGAAGACGCGTGTGATTGTTAAACGACCCGTCTCGGCCAGTCTGGCCCGGGCC‑TTTTTTACATTGTGCTGCTGTCGATTCttt                                                            <  1:754486/93‑1 (MQ=255)

                              tttttGTGGAGAAGACGCGTGTGATTGTTAAACGACCCGTCTCGGCCAGTCTGGCCCGGGCC‑TTTTTTACATTGTGCTGCTGTCGATTCTTTc                                                           <  2:4461483/93‑1 (MQ=255)

                               ttttGTGGAGAAGACGCGTGTGATTGTTAAACGACCCGTCTCGGCCAGTCTGGCCCGGGCC‑TTTTTTACATTGTGCTGCTGTCGATTCTTTcc                                                          <  1:1178925/93‑1 (MQ=255)

                                  tgtgGAGAAGACGCGTGTGATTGTTAAACGACCCGTCTCGGCCAGTCTGGCCCGGGCC‑TTTTTTACATTGTGCTGCTGTCGATTCTTTCCACg                                                       >  1:1167992/1‑93 (MQ=255)

                                   gtgGAGAAGACGCGTGTGATTGTTAAACGACCCGTCTCGGCCAGTCTGGCCCGGGCC‑TTTTTTACATTGTGCTGCTGTCGATTCTTTCCACgg                                                      >  1:3919799/1‑93 (MQ=255)

                                     ggAGAAGACGCGTGTGATTGTTAAACGACCCGTCTCGGCCAGTCTGGCCCGGGCC‑TTTTTTACATTGTGCTGCTGTCGATTCTTTCCACGGGt                                                    >  1:4993193/1‑93 (MQ=255)

                                     ggAGAAGACGCGTGTGATTGTTAAACGACCCGTCTCGGCCAGTCTGGCCCGGGCC‑TTTTTTACATTGTGCTGCTGTCGATTATTTCCACGGGt                                                    >  2:4984829/1‑93 (MQ=255)

                                       agaagaCGCGTGTGATTGTTAAACGACCCGTCTCGGCCAGTCTGGCCCGGGCC‑TTTTTTACATTGTGCTGCTGTCGATTCTTTCCACGGGTAt                                                  <  1:5591863/93‑1 (MQ=255)

                                        gaagaCGCGTGTGGTTGTTAAACGACCCGTCTCGGCCAGTCTGGCCCGGGCC‑TTTTTTACATTGTGCTGCTGTCGATTCTTTCCACGGGTATc                                                 <  1:4893988/93‑1 (MQ=255)

                                        gaagaCGCGTGTGATTGTTAAACGACCCGTCTCGGCCAGTCTGGGCCGGGCC‑TTTTTTACATTGTGCTGCTGTCGATTCTTTCCACGGGTGTc                                                 >  2:1467652/1‑93 (MQ=255)

                                        gaagaCGCGTGTGATTGTTAAACGACCCGTCTCGGCCAGTCTGGCCCGGGCC‑TTTTTTACATTGTGCTGCTGTCGATTCTTTCCACGGGTATc                                                 >  2:3079028/1‑93 (MQ=255)

                                         aagaCGCGTGTGATTGTTAAACGACCCGTCTCGGCCAGTCTGGCCCGGGCC‑TTTTTTACATTGTGCTGCTGTCGATTCTTTCCACGGGTATCg                                                >  1:4666540/1‑93 (MQ=255)

                                          agaCGCGTGTGATTGTTAAACGACCCGTCTCGGCCAGTCTGGCCCGGGCC‑TTTTTTACATTGTGCTGCTGTCGATTCTTTCCACGGGTATCGc                                               <  1:2087872/93‑1 (MQ=255)

                                          agaCGCGTGTGATTGTTAAACGACCCGTCTCGGCCAGTCTGGCCCGGGCC‑TTTTTTACATTGTGCTGCTGTCGATTCTTTCCACGGGTATCGc                                               <  1:1785940/93‑1 (MQ=255)

                                           gaCGCGTGTGATTGTTAAACGACCCGTCTCGGCCAGTCTGGCCCGGGCC‑TTTTTTACATTGTGCTGCTGTCGATTCTTTCCACGGGTATCGct                                              <  2:765606/93‑1 (MQ=255)

                                            aCGCGTGTGATTGTTAAACGACCCGTCTCGGCCAGTCTGGCCCGGGCC‑TTTTTTACATTGTGCTGCTGTCGATTCTTTCCACGGGTATCGctc                                             <  1:5582524/93‑1 (MQ=255)

                                               cgTGTGATTGTTAAACGACCCGTCTCGGCCAGTCTGGCCCGGGCC‑TTTTTTACATTGTGCTGCTGTCGATTCTTTCCACGGGTATCGCTCTGc                                          <  2:2707346/93‑1 (MQ=255)

                                               cgTGTGATTGTTAAACGACACGTCTCGGCCAGTCTGGCCCGGGCC‑TTTTTTACATTGTGCTGCTGTCGATTCTTTCCACGGGTATCGCTCTGc                                          >  2:6153381/1‑93 (MQ=255)

                                                     aTTGTTAAACGACCCGTCTCGGCCAGTCTGGCCCGGGCC‑TTTTTTACATTGTGCTGCTGTCGATTCTTTCCACGGGTATCGCTCTGCTAActc                                    <  1:2147435/93‑1 (MQ=255)

                                                      ttGTTAAACGACCCGTCTCGGCCAGTCTGGCCCGGGCC‑TTTTTTACATTGTGCTGCTGTCGATTCTTTCCACGGGTATCGCTCTGCTAActct                                   <  1:2019269/93‑1 (MQ=255)

                                                       tGTTAAACGACCCGTCTCGGCCAGTCTGGCCCGGGCC‑TTTTTTACATTGTGCTGCTGTCGATTCTTTCCACGGGTATCGCTCTGCTAACTCTg                                  >  2:5779085/1‑93 (MQ=255)

                                                       tGTTAAACGACCCGTCTCGGCCAGTCTGGCCCGGGCC‑TTTTTTACATTGTGCTGCTGTCGATTCTTTCCACGGGTATCGCTCTGCTAACTCTg                                  <  2:5661118/93‑1 (MQ=255)

                                                        gTTAAACGACCCGTCTCGGCCAGTCTGGCCCGGGCC‑TTTTTTACATTGTGCTGCTGTCGATTCTTTCCACCGGGATCGCTCTGCTCACTCTgg                                 >  2:5642722/1‑93 (MQ=255)

                                                         ttAAACGACCCGTCTCGGCCAGTCTGGCCCGGGCC‑TTTTTTACATTGTGCTGCTGTCGATTCTTTCCACGGGTATCGCTCTGCTAACTCTGGc                                <  1:5072874/93‑1 (MQ=255)

                                                           aaaCGACCCGTCTCGGCCAGTCTGGCCCGGGCC‑TTTTTTACATTGTGCTGCTGTCGATTCTTTCCACGGGTATCGCTCTGCTAACTCTGGCGa                              <  1:385665/93‑1 (MQ=255)

                                                             aCGACCCGTCTCGGCCAGTCTGGCCCGGGCC‑TTTTTTACATTGTGCTGCTGTCGATTCTTTCCACGGGTATCGCTCTGCTAACTCTGGCGAGc                            <  2:1443825/93‑1 (MQ=255)

                                                             aCGACCCGTCTCGGCCAGTCTGGCCCGGGCC‑TTTTTTACATTGTGCTGCTGTCGATTCTTTCCACGGGTATCGCTCTGCTAACTCTGGCGAGc                            >  2:1885424/1‑93 (MQ=255)

                                                                aCCCGTCTCGGCCAGTCTGGCCCGGGCC‑TTTTTTACATTGTGCTGCTGTCGATTCTTTCCACGGGTATCGCTCTGCTAACTCTGGCGAGCAGt                         >  2:2415688/1‑93 (MQ=255)

                                                                 cccGTCTCGGCCAGTCTGGCCCGGGCC‑TTTTTTACATTGTGCTGCTGTCGATTCTTTCCACGGGTATCGCTCTGCTAACTCTGGCGAGCAGtt                        >  1:4025028/1‑93 (MQ=255)

                                                                 cccGTCTCGGCCAGTCTGGCCCGGGCC‑TTTTTTACATTGTGCTGCTGTCGATTCTTTCCACGGGTATCGCTCTGCTAACTCTGGCGAGCAGtt                        <  2:2655967/93‑1 (MQ=255)

                                                                 cccGTCTCGGCCAGTCTGGCCCGGGCC‑TTTTTTACATTGTGCTGCTGTCGATTCTTTCCACGGGTATCGCTCTGCTAACTCTGGCGAGCAGtt                        >  2:3478629/1‑93 (MQ=255)

                                                                 cccGTCTCGGCCAGTCTGGCCCGGGCC‑TTTTTTACATTGTGCTGCTGTCGATTCTTTCCACGGGTATCGCTCTGCTAACTCTGGCGAGCAGtt                        >  1:3172930/1‑93 (MQ=255)

                                                                 cccGTCTCGGCCAGTCTGGCCCGGGCC‑TTTTTTACATTGTGCTGCTGTCGATTCTTTCCACGGGTATCGCTCTGCTAACTCTGGCGAGCAGtt                        <  1:5172143/93‑1 (MQ=255)

                                                                  ccGTCTCGGCCAGTCTGGCCCGGGCC‑TTTTTTACATTGTGCTGCTGTCGATTCTTTCCACGGGTATCGCTCTGCTAACTCTGGCGAGCAGttt                       <  1:2612986/93‑1 (MQ=255)

                                                                  ccGTCTCGGCCAGTCTGGCCCGGGCC‑TTTTTTACATTGTGCTGCTGTCGAGTCTTTCCACGGGTATCGCTCTGCTATCTCTGGCGAGCAGttt                       >  1:2886784/1‑93 (MQ=255)

                                                                    gTCTCGGCCAGTCTGGCCCGGGCC‑TTTTTTACATTGTGCTGCTGTCGATTCTTTCCACGGGTATCGCTCTGCTAACTCTGGCGAGCAGTTTgc                     >  1:4768400/1‑93 (MQ=255)

                                                                    gTCTCGGCCAGTCTGGCCCGGGCC‑TTTTTTACATTGTGCTGCTGTCGATTCTTTCCACGGGTATCGCTCTGCTAACTCTGGCGAGCAGTTTgc                     <  1:1488701/93‑1 (MQ=255)

                                                                     tctcGGCCAGTCTGGCCCGGGCC‑TTTTTTACATTGTGCTGCTGTCGATTCTTTCCACGGGTATCGCTCTGCTAACTCTGGCGAGCAGTTTgcg                    >  1:722809/1‑93 (MQ=255)

                                                                     tctcGGCCAGTCTGGCCCGGGCC‑TTTTTTACATTGTGCTGCTGTCGATTCTTTCCACGGGTATCGCTCTGCTAACTCTGGCGAGCAGTTTgcg                    >  2:2768030/1‑93 (MQ=255)

                                                                     tctcGGCCAGTCTGGCCCGGGCC‑TTTTTTACATTGTGCTGCTGTCGATTCTTTCCACGGGTATCGCTCTGCTAACTCTGGCGAGCAGTTTgcg                    >  1:433421/1‑93 (MQ=255)

                                                                     tctcGGCCAGTCTGGCCCGGGCC‑TTTTTTACATTGTGCTGCTGTCGATTCTTTCCACGGGTATCGCTCTGCTAACTCTGGCGAGCAGTTTgcg                    >  1:497904/1‑93 (MQ=255)

                                                                      ctcGGCCAGTCTGGCCCGGGCC‑TTTTTTACATTGTGCTGCTGTCGATTCTTTCCACGGGTATCGCTCTGCTAACTCTGGCGAGCAGTTTgcgc                   <  1:481784/93‑1 (MQ=255)

                                                                        cGGCCAGTCTGGCCCGGGCC‑TTTTTTACATTGTGCTGCTGTCGATTCTTTCCACGGGTATCGCTCTGCTAACTCTGGCGAGCAGTTTGCGCGa                 <  2:1242039/93‑1 (MQ=255)

                                                                         ggCCAGTCTGGCCCGGGCC‑TTTTTTACATTGTGCTGCTGTCGATTCTTTCCACGGGTATCGCTCTGCTAACTCTGGCGAGCAGTTTGCGCGAc                <  2:3177906/93‑1 (MQ=255)

                                                                         ggCCAGTCTGGCCCGGGCC‑TTTTTTACATTGTGCTGCTGTCGATTCTTTCCACGGGTATCGCTCTGCTAACTCTGGCGAGCAGTTTGCGCGAc                >  1:5681223/1‑93 (MQ=255)

                                                                           ccAGTCTGGCCCGGGCC‑TTTTTTACATTGTGCTGCTGTCGATTCTTTCCACGGGTATCGCTCTGCTAACTCTGGCGAGCAGTTTGCGCGACGc              <  2:287057/93‑1 (MQ=255)

                                                                           ccAGTCTGGCCCGGGCC‑TTTTTTACATTGTGCTGCTGTCGATTCTTTCCACGGGTATCGCTCTGCTAACTCTGGCGAGCAGTTTGCGCGACGc              <  1:1411406/93‑1 (MQ=255)

                                                                            cAGTCTGGCCCGGGCC‑TTTTTTACATTGTGCTGCTGTCGATTCTTTCCACGGGTATCGCTCTGCTAACTCTGGCGAGCAGTTTGCGCGACGCt             <  2:5621561/93‑1 (MQ=255)

                                                                            cAGTCTGGCCCGGGCC‑TTTTTTACATTGTGCTGCTGTCGATTCTTTCCACGGGTATCGCTCTGCTAACTCTGGCGAGCAGTTTGCGCGACGCt             >  2:4591560/1‑93 (MQ=255)

                                                                             aGTCTGGCCCGGGCC‑TTTTTTACATTGTGCTGCTGTCGATTCTTTCCACGGGTATCGCTCTGCTAACTCTGGCGAGCAGTTTGCGCGACGCTg            <  2:3375015/93‑1 (MQ=255)

                                                                              gTCTGGCCCGGGCC‑TTTTTTACATTGTGCTGCTGTCGATTCTTTCCACGGGTATCGCTCTGCTAACTCTGGCGAGCAGTTTGCGCGACGCTGa           <  2:3582349/93‑1 (MQ=255)

                                                                              gTCTGGCCCGGGCC‑TTTTTTACATTGTGCTGCTGTCGATTCTTTCCACGGGTATCGCTCTGCTAACTCTGGCGAGCAGTTTGCGCGACGCTGa           <  1:5287649/93‑1 (MQ=255)

                                                                               tCTGGCCCGGGCC‑TTTTTTACATTGTGCTGCTGTCGATTCTTTCCACGGGTATCGCTCTGCTAACTCTGGCGAGCAGTTTGCGCGACGCTGAg          <  2:946947/93‑1 (MQ=255)

                                                                               tCTGGCCCGGGCC‑TTTTTTACATTGTGCTGCTGTCGATTCTTTCCACGGGTATCGCTCTGCTAACTCTGGCGAGCAGTTTGCGCGACGCTGAg          <  2:3837744/93‑1 (MQ=255)

                                                                               tCTGGCCCGGGCC‑TTTTTTACATTGTGCTGCTGTCGATTCTTTCCACGGGTATCGCTCTGCTAACTCTGGCGAGCAGTTTGCGCGACGCTGAg          >  1:5322337/1‑93 (MQ=255)

                                                                               tCTGGCCCGGGCC‑TTTTTTACATTGTGCTGCTGTCGATTCTTTCCACGGGTATCGCTCTGCTAACTCTGGCGAGCAGTTTGCGCGACGCTGAg          >  1:3736401/1‑93 (MQ=255)

                                                                                cTGGCCCGGGCC‑TTTTTTACATTGTGCTGCTGTCGATTCTTTCCACGGGTATCGCTCTGCTAACTCTGGCGAGCAGTTTGCGCGACGCTGAgg         >  2:636258/1‑93 (MQ=255)

                                                                                 tGGCCCGGGCC‑TTTTTTACATTGTGCTGCTGTCGATTCTTTCCACGGGTATCGCTCTGCTAACTCTGGCGAGCAGTTTGCGCGACGCTGAGGc        <  2:1040577/93‑1 (MQ=255)

                                                                                   gCCCGGGCC‑TTTTTTACATTGTGCTGCTGTCGATTCTTTCCACGGGTATCGCTCTGCTAACTCTGGCGAGCAGTTTGCGCGACGCTGAGGCTa      <  2:4684633/93‑1 (MQ=255)

                                                                                   gCCCGGGCC‑TTTTTTACATTGTGCTGCTGTCGATTCTTTCCACGGGTATCGCTCTGCTAACTCTGGCGAGCAGTTTGCGCGACGCTGAGGCTa      <  2:3031701/93‑1 (MQ=255)

                                                                                   gCCCGGGCC‑TTTTTTACATTGTGCTGCTGTCGATTCTTTCCACGGGTATCGCTCTGCTAACTCTGGCGAGCAGTTTGCGCGACGCTGAGGCTa      <  2:6039603/93‑1 (MQ=255)

                                                                                   gCCCGGGCC‑TTTTTTACATTGTGCTGCTGTCGATTCTTTCCACGGGTATCGCTCTGCTAACTCTGGCGAGCAGTTTGCGCGACGCTGAGGCTa      >  1:1052129/1‑93 (MQ=255)

                                                                                    cccGGGCC‑TTTTTTACATTGTGCTGCTGTCGATTCTTTCCACGGGTATCGCTCTGCTAACTCTGGCGAGCAGTTTGCGCGACGCTGAGGCTAt     >  2:197759/1‑93 (MQ=255)

                                                                                     ccGGGCC‑TTTTTTACATTGTGCTGCTGTCGATTCTTTCCACGGGTATCGCTCTGCTAACTCTGGCGAGCAGTTTGCGCGACGCTGAGGCTATc    <  2:106544/93‑1 (MQ=255)

                                                                                     ccGGGCC‑TTTTTTACATTGTGCTGCTGTCGATTCTTTCCACGGGTATCGCTCTGCTAACTCTGGCGAGCAGTTTGCGCGACGCTGAGGCTATc    <  1:4154443/93‑1 (MQ=255)

                                                                                     ccGGGCC‑TTTTTTACATTGTGCTGCTGTCGATTCTTTCCACGGGTATCGCTCTGCTAACTCTGGCGAGCAGTTTGCGCGACGCTGAGGCTATc    <  1:2452745/93‑1 (MQ=255)

                                                                                      cGGGCC‑TTTTTTACATTGTGCTGCTGTCGATTCTTTCCACGGGTATCGCTCTGCTAACTCTGGCGAGCAGTTTGCGCGACGCTGAGGCTATCa   >  2:1754475/1‑93 (MQ=255)

                                                                                      cGGGCC‑TTTTTTACATTGTGCTGCTGTCGATTCTTTCCACGGGTATCGCTCTGCTAACTCTGGCGAGCAGTTTGCGCGACGCTGAGGCTATCa   >  2:1019102/1‑93 (MQ=255)

                                                                                       gggCC‑TTTTTTACATTGTGCTGCTGTCGATTCTTTCCACGGGTATCGCTCTGCTAACTCTGGCGAGCAGTTTGCGCGACGCTGAGGCTATCaa  >  2:4790234/1‑93 (MQ=255)

TGCGTTTCAGCGAACTGGAACATTAATGATTTTTTGTGGAGAAGACGCGTGTGATTGTTAAACGACCCGTCTCGGCCAGTCTGGCCCGGGCCTTTTTTTACATTGTGCTGCTGTCGATTCTTTCCACGGGTATCGCTCTGCTAACTCTGGCGAGCAGTTTGCGCGACGCTGAGGCTATCAA  >  NC_000913/2583701‑2583881

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

CAGCGAACTGGAACATTAATGATTTTTTGTGGAGAAGACGCGTGTGATTGTTAAACGACCCGTCTCGGCCAGTCTGGCCCGGGCCTTTTTTTACATTGTGCTGCTGTCGATTCTTTCCACGGGTATCGCTCTGCTAACTCTGGCGAGCAGTTTGCGCGACGCTGAGGCTATCAATATTGCCGGAT  >  NC_000913/2583708‑2583892

CAGCGAACTGGAACATTAATGATTTTTTGTGGAGAAGACGCGTGTGATTGTTAAACGACCCGTCTCGGCCAGTCTGGCCCGGGCC‑TTTTTTACA                                                                                            >  SRR4302533.223689/1‑94 (MQ=60)

CAGCGAACTGGAACATTAATGATTTTTTGTGGAGAAGACGCGTGTGATTGTTAAACGACCCGTCTCGGCCAGTCTGGCCCGGGCC‑TTTTTTACA                                                                                            >  SRR4302533.5130383/1‑94 (MQ=60)

 AGCGAACTGGAACATTAATGATTTTTTGTGGAGAAGACGCGTGTGATTGTTAAACGACCCGTCTCGGCCAGTCTGGCCCGGGCC‑TTTTTTACAT                                                                                           >  SRR4302533.2687599/1‑94 (MQ=60)

   CGAACTGGAACATTAATGATTTTTTGTGGAGAAGACGCGTGTGATTGTTAAACGACCCGTCTCGGCCAGTCTGGCCCGGGCC‑TTTTTTACATTG                                                                                         <  SRR4302533.551467/94‑1 (MQ=60)

    GAACTGGAACATTAATGATTTTTTGTGGAGAAGACGCGTGTGATTGTTAAACGACCCGTCTCGGCCAGTCTGGCCCGGGCC‑TTTTTTACATTGT                                                                                        >  SRR4302533.308824/1‑94 (MQ=60)

    GAACTGGAACATTAATGATTTTTTGTGGAGAAGACGCGTGTGATTGTTAAACGACCCGTCTCGGCCAGTCTGGCCCGGGCC‑TTTTTTACATTGT                                                                                        <  SRR4302533.82549/94‑1 (MQ=60)

    GAACTGGAACATTAATGATTTTTTGTGGAGAAGACGCGTGTGATTGTTAAACGACCCGTCTCGGCCAGTCTGGCCCGGGCC‑TTTTTTACATTGT                                                                                        >  SRR4302533.4494622/1‑94 (MQ=60)

     AACTGGAACATTAATGATTTTTTGTGGAGAAGACGCGTGTGATTGTTAAACGACCCGTCTCGGCCAGTCTGGCCCGGGCC‑TTTTTTACATTGTG                                                                                       >  SRR4302533.3676935/1‑94 (MQ=60)

     AACTGGAACATTAATGATTTTTTGTGGAGAAGACGCGTGTGATTGTTAAACGACCCGTCTCGACCAGTCTGGCCCGGGCC‑TTTTTTACATTGTG                                                                                       >  SRR4302533.5697332/1‑94 (MQ=60)

      ACTGGAACATTAATGATTTTTTGTGGAGAAGACGCGTGTGATTGTTAAACGACCCGTCTCGGCCAGTCTGGCCCGGGCC‑TTTTTTACATTGTGC                                                                                      >  SRR4302533.166639/1‑94 (MQ=60)

       CTGGAACATTAATGATTTTTTGTGGAGAAGACGCGTGTGATTGTTAAACGACCCGTCTCGGCCAGTCTGGCCCGGGCC‑TTTTTTACATTGTGCT                                                                                     >  SRR4302533.6062469/1‑94 (MQ=60)

          GAACATTAATGATTTTTTGTGGAGAAGACGCGTGTGATTGTTAAACGACCCGTCTCGGCCAGTCTGGCCCGGGCC‑TTTTTTACATTGTGCTGCT                                                                                  >  SRR4302533.3919977/1‑94 (MQ=60)

          GAACATTAATGATTTTTTGTGGAGAAGACGCGTGTGATTGTTAAACGACCCGTCTCGGCCAGTCTGGCCCGGGCC‑TTTTTTACATTGTGCTGCT                                                                                  <  SRR4302533.4473204/94‑1 (MQ=60)

           AACATTAATGATTTTTTGTGGAGAAGACGCGTGTGATTGTTAAACGACCCGTCTCGGCCAGTCTGGCCCGGGCC‑TTTTTTACATTGTGCTGCTG                                                                                 <  SRR4302533.5271003/94‑1 (MQ=60)

           AACATTAATGATTTTTTGTGGAGAAGACGCGTGTGATTGTTAAACGACCCGTCTCGGCCAGTCTGGCCCGGGCC‑TTTTTTACATTGTGCTGCTG                                                                                 >  SRR4302533.4000030/1‑94 (MQ=60)

            ACATTAATGATTTTTTGTGGAGAAGACGCGTGTGATTGTTAAACGACCCGTCTCGGCCAGTCTGGCCCGGGCC‑TTTTTTACATTGTGCTGCTGT                                                                                >  SRR4302533.5665339/1‑94 (MQ=60)

            ACATTAATGATTTTTTGTGGAGAAGACGCGTGTGATTGTTAAACGACCCGTCTCGGCCAGTCTGGCCCGGGCC‑TTTTTTACATTGTGCTGCTGT                                                                                >  SRR4302533.2355459/1‑94 (MQ=60)

             CATTAATGATTTTTTGTGGAGAAGACGCGTGTGATTGTTAAACGACCCGTCTCGGCCAGTCTGGCCCGGGCC‑TTTTTTACATTGTGCTGCTGTC                                                                               >  SRR4302533.745701/1‑94 (MQ=60)

              ATTAATGATTTTTTGTGGAGAAGACGCGTGTGATTGTTAAACGACCCGTCTCGGCCAGTCTGGCCCGGGCC‑TTTTTTACATTGTGCTGCTGTCG                                                                              <  SRR4302533.1860914/94‑1 (MQ=60)

               TTAATGATTTTTTGTGGAGAAGACGCGTGTGATTGTTAAACGACCCGTCTCGGCCAGTCTGGCCCGGGCC‑TTTTTTACATTGTGCTGCTGTCGA                                                                             >  SRR4302533.4374112/1‑94 (MQ=60)

               TTAATGATTTTTTGTGGAGAAGACGCGTGTGATTGTTAAACGACCCGTCTCGGCCAGTCTGGCCCGGGCC‑TTTTTTACATTGTGCTGCTGTCGA                                                                             >  SRR4302533.4962750/1‑94 (MQ=60)

                TAATGATTTTTTGTGGAGAAGACGCGTGTGATTGTTAAACGACCCGTCTCGGCCAGTCTCGCCCGGGCC‑TTTTTTACATTTTGCTGCTGTCGAT                                                                            >  SRR4302533.4639544/1‑94 (MQ=60)

                  ATGATTTTTTGTGGAGAAGACGCGTGTGATTGTTAAACGACCCGTCTCGGCCAGTCTGGCCCGGGCC‑TTTTTTACATTGTGCTGCTGTCGATTC                                                                          <  SRR4302533.3031346/94‑1 (MQ=60)

                   TGATTTTTTGTGGAGAAGACGCGTGTGATTGTTAAACGACCCGTCTCGGCCAGTCTGGCCCGGGCC‑TTTTTTACATTGTGCTGCTGTCGATTCT                                                                         >  SRR4302533.441565/1‑94 (MQ=60)

                   TGATTTTTTGTGGAGAAGACGCGTGTGATTGTTAAACGACCCGTCTCGGCCAGTCTGGCCCGGGCC‑TTTTTTACATTGTGCTGCTGTCGATTCT                                                                         <  SRR4302533.195514/94‑1 (MQ=60)

                   TGATTTTTTGTGGAGAAGACGCGTGTGATTGTTAAACGACCCGTCTCGGCCAGTCTGGCCCGGGCC‑TTTTTTACATTGTGCTGCTGTCGATTCT                                                                         >  SRR4302533.6189859/1‑94 (MQ=60)

                    GATTTTTTGTGGAGAAGACGCGTGTGATTGTTAAACGACCCGTCTCGGCCAGTCTGGCCCGGGCC‑TTTTTTACATTGTGCTGCTGTCGATTCTT                                                                        <  SRR4302533.2416312/94‑1 (MQ=60)

                      TTTTTTGTGGAGAAGACGCGTGTGATTGTTAAACGACCCGTCTCGGCCAGTCTGGCCCGGGCC‑TTTTTTACATTGTGCTGCTGTCGATTCTTTC                                                                      <  SRR4302533.758926/94‑1 (MQ=60)

                        TTTTGTGGAGAAGACGCGTGTGATTGTTAAACGACCCGTCTCGGCCAGTCTGGCCCGGGCC‑TTTTTTACATTGTGCTGCTGTCGATTCTTTCCA                                                                    <  SRR4302533.4204522/94‑1 (MQ=60)

                        TTTTGTGGAGAAGACGCGTGTGATTGTTAAACGACCCGTCTCGGCCAGTCTGGCCCGGGCC‑TTTTTTACATTGTGCTGCTGTCGATTCTTTCCA                                                                    <  SRR4302533.1186004/94‑1 (MQ=60)

                         TTTGTGGAGAAGACGCGTGTGATTGTTAAACGACCCGTCTCGGCCAGTCTGGCCCGGGCC‑TTTTTTACATTGTGCTGCTGTCGATTCTTTCCAC                                                                   <  SRR4302533.4233090/94‑1 (MQ=60)

                         TTTGTGGAGAAGACGCGTGTGATTGTTAAACGACCCGTCTCGGCCAGTCTGGCCCGGGCC‑TTTTTTACATTGTGCTGCTGTCGATTCTTTCCAC                                                                   <  SRR4302533.2094647/94‑1 (MQ=60)

                         TTTGTGGAGAAGACGCGTGTGATTGTTAAACGACCCGTCTCGGCCAGTCTGGCCCGGGCC‑TTTTTTACATTGTGCTGCTGTCGATTCTTTCCAC                                                                   >  SRR4302533.1484486/1‑94 (MQ=60)

                          TTGTGGAGAAGACGCGTGTGATTGTTAAACGACCCGTCTCGGCCAGTCTGGCCCGGGCC‑TTTTTTACATTGTGCTGCTGTCGATTCTTTCCACG                                                                  >  SRR4302533.1174999/1‑94 (MQ=60)

                           TGTGGAGAAGACGCGTGTGATTGTTAAACGACCCGTCTCGGCCAGTCTGGCCCGGGCC‑TTTTTTACATTGTGCTGCTGTCGATTCTTTCCACGG                                                                 >  SRR4302533.3943736/1‑94 (MQ=60)

                           TGTGGAGAAGACGCGTGTGATTGTTAAACGACCCGTCTCGGCCAGTCTGGCCCGGGCC‑TTTTTTACATTGTGCTGCTGTCGATTCTTTCCACGG                                                                 <  SRR4302533.5003325/94‑1 (MQ=60)

                             TGGAGAAGACGCGTGTGATTGTTAAACGACCCGTCTCGGCCAGTCTGGCCCGGGCC‑TTTTTTACATTGTGCTGCTGTCGATTCTTTCCACGGGT                                                               >  SRR4302533.5651460/1‑94 (MQ=60)

                             TGGAGAAGACGCGTGTGATTGTTAAACGACCCGTCTCGGCCAGTCTGGCCCGGGCC‑TTTTTTACATTGTGCTGCTGTCGATTCTTTCCACGGGT                                                               >  SRR4302533.5023465/1‑94 (MQ=60)

                               GAGAAGACGCGTGTGATTGTTAAACGACCCGTCTCGGCCAGTCTGGCCCGGGCC‑TTTTTTACATTGTGCTGCTGTCGATTCTTTCCACGGGTAT                                                             <  SRR4302533.242897/94‑1 (MQ=60)

                                AGAAGACGCGTGTGATTGTTAAACGACCCGTCTCGGCCAGTCTGGCCCGGGCC‑TTTTTTACATTGTGCTGCTGTCGATTCTTTCCACGGGTATC                                                            <  SRR4302533.5625644/94‑1 (MQ=60)

                                 GAAGACGCGTGTGGTTGTTAAACGACCCGTCTCGGCCAGTCTGGCCCGGGCC‑TTTTTTACATTGTGCTGCTGTCGATTCTTTCCACGGGTATCG                                                           <  SRR4302533.4923649/94‑1 (MQ=60)

                                 GAAGACGCGTGTGATTGTTAAACGACCCGTCTCGGCCAGTCTGGCCCGGGCC‑TTTTTTACATTGTGCTGCTGTCGATTCTTTCCACGGGTATCG                                                           <  SRR4302533.1140090/94‑1 (MQ=60)

                                 GAAGACGCGTGTGATTGTTAAACGACCCGTCTCGGCCAGTCTGGCCCGGGCC‑TTTTTTACATTGTGCTGCTGTCGATTCTTTCCACGGGTATCG                                                           >  SRR4302533.4694813/1‑94 (MQ=60)

                                 GAAGACGCGTGTGATTGTTAAACGACCCGTCTCGGCCAGTCTGGCCCGGGCC‑TTTTTTACATTGTGCTGCTGTCGATTCTTTCCACGGGTATCG                                                           <  SRR4302533.3084492/94‑1 (MQ=60)

                                   AGACGCGTGTGATTGTTAAACGACCCGTCTCGGCCAGTCTGGCCCGGGCC‑TTTTTTACATTGTGCTGCTGTCGATTCTTTCCACGGGTATCGCT                                                         >  SRR4302533.4797493/1‑94 (MQ=60)

                                   AGACGCGTGTGATTGTTAAACGACCCGTCTCGGCCAGTCTGGCCCGGGCC‑TTTTTTACATTGTGCTGCTGTCGATTCTTTCCACGGGTATCGCT                                                         <  SRR4302533.2100728/94‑1 (MQ=60)

                                   AGACGCGTGTGATTGTTAAACGACCCGTCTCGGCCAGTCTGGCCCGGGCC‑TTTTTTACATTGTGCTGCTGTCGATTCTTTCCACGGGTATCGCT                                                         <  SRR4302533.1796800/94‑1 (MQ=60)

                                     ACGCGTGTGATTGTTAAACGACCCGTCTCGGCCAGTCTGGCCCGGGCC‑TTTTTTACATTGTGCTGCTGTCGATTCTTTCCACGGGTATCGCTCT                                                       <  SRR4302533.5616253/94‑1 (MQ=60)

                                      CGCGTGTGATTGTTAAACGACCCGTCTCGGCCAGTCTGGCCCGGGCC‑TTTTTTACATTGTGCTGCTGTCGATTCTTTCCACGGGTATCGCTCTG                                                      <  SRR4302533.5779290/94‑1 (MQ=60)

                                        CGTGTGATTGTTAAACGACCCGTCTCGGCCAGTCTGGCCCGGGCC‑TTTTTTACATTGTGCTGCTGTCGATTCTTTCCACCGGGATCGCTCTGCT                                                    >  SRR4302533.5332610/1‑94 (MQ=60)

                                         GTGTGATTGTTAAACGACCCGTCTCGGCCAGTCTGGCCCGGGCC‑TTTTTTACATTGTGCTGCTGTCGATTCTTTCCACGGGTATCGCTCTGCTA                                                   <  SRR4302533.5701704/94‑1 (MQ=60)

                                         GTGTGATTGTTAAACGACCCGTCTCGGCCAGTCTGGCCCGGGCC‑TTTTTTACATTGTGCTGCTGTCGATTCTTTCCACGGGTATCGCTCTGCTA                                                   >  SRR4302533.2147750/1‑94 (MQ=60)

                                         GTGTGATTGTTAAACGACCCGTCTCGGCCAGTCTGGCCCGGGCC‑TTTTTTACATTGTGCTGCTGTCGATTCTTTCCACGGGTATCGCTCTGCTA                                                   >  SRR4302533.521304/1‑94 (MQ=60)

                                         GTGTGATTGTTAAACGACCCGTCTCGGCCAGTCTGGCCCGGGCC‑TTTTTTACATTGTGCTGCTGTCGATTCTTTCCACGGGCATCGCTCTGCTA                                                   >  SRR4302533.3861154/1‑94 (MQ=60)

                                         GTGTGATTGTTAAACGACCCGTCTCGGCCAGTCTGGCCCGGGCC‑TTTTTTACATTGTGCTGCTCTCGATTCTTTCCACGGCGATCGCTCTGCTA                                                   >  SRR4302533.4782469/1‑94 (MQ=60)

                                          TGTGATTGTTAAACGACCCGTCTCGGCCAGTCTGGCCCGGGCC‑TTTTTTACATTGTGCTGCTGTCGATTCTTTCCACGGGTATCGCTCTGCTAA                                                  >  SRR4302533.4861982/1‑94 (MQ=60)

                                            TGATTGTTAAACGACCCGTCTCGGCCAGTCTGGCCCGGGCC‑TTTTTTACATTGTGCTGCTGTCGATTCTTTCCACGGGTATCGCTCTGCTAACT                                                <  SRR4302533.5547242/94‑1 (MQ=60)

                                            TGATTGTTAAACGACCCGTCTCGGCCAGTCTGGCCCGGGCC‑TTTTTTACATTGTGCTGCTGTCGATTCTTTCCACGGGTATCGCTCTGCTAACT                                                >  SRR4302533.370746/1‑94 (MQ=60)

                                            TGATTGTTAAACGACCCGTCTCGGCCAGTCTGGCCCGGGCC‑TTTTTTACATTGTGCTGCTGTCGATTCTTTCCACGGGTATCGCTCTGCTAACT                                                >  SRR4302533.4630711/1‑94 (MQ=60)

                                             GATTGTTAAACGACCCGTCTCGGCCAGTCTGGCCCGGGCC‑TTTTTTACATTGTGCTGCTGTCGATTCTTTCCACGGGTATCGCTCTGCTAACTC                                               <  SRR4302533.5022838/94‑1 (MQ=60)

                                             GATTGTTAAACGACCCGTCTCGGCCAGTCTGGCCCGGGCC‑TTTTTTACATTGTGCTGCTGTCGATTCTTTCCACGGGTATCGCTCTGCTAACTC                                               >  SRR4302533.3026776/1‑94 (MQ=60)

                                              ATTGTTAAACGACCCGTCTCGGCCAGTCTGGCCCGGGCC‑TTTTTTACATTGTGCTGCTGTCGATTCTTTCCACGGGTATCGCTCTGCTAACTCT                                              <  SRR4302533.2160659/94‑1 (MQ=60)

                                               TTGTTAAACGACCCGTCTCGGCCAGTCTGGCCCGGGCC‑TTTTTTACATTGTGCTGCTGTCGATTCTTTCCACGGGTATCGCTCTGCTAACTCTG                                             <  SRR4302533.3032839/94‑1 (MQ=60)

                                               TTGTTAAACGACCCGTCTCGGCCAGTCTGGCCCGGGCC‑TTTTTTACATTGTGCTGCTGTCGATTCTTTCCACGGGTATCGCTCTGCTAACTCTG                                             <  SRR4302533.2031706/94‑1 (MQ=60)

                                                TGTTAAACGACCCGTCTCGGCCAGTCTGGCCCGGGCC‑TTTTTTACATTGTGCTGCTGTCGATTCTTTCCACGGGTATCGCTCTGCTAACTCTGG                                            <  SRR4302533.2055853/94‑1 (MQ=60)

                                                TGTTAAACGACCCGTCTCGGCCAGTCTGGCCCGGGCC‑TTTTTTACATTGTGCTGCTGTCGATTCTTTCCACGGGTATCGCTCTGCTAACTCTGG                                            <  SRR4302533.4867655/94‑1 (MQ=60)

                                                  TTAAACGACCCGTCTCGGCCAGTCTGGCCCGGGCC‑TTTTTTACATTGTGCTGCTGTCGATTCTTTCCACGGGTATCGCTCTGCTAACTCTGGCG                                          >  SRR4302533.1176869/1‑94 (MQ=60)

                                                  TTAAACGACCCGTCTCGGCCAGTCTGGCCCGGGCC‑TTTTTTACATTGTGCTGCTGTCGATTCTTTCCACGGGTATCGCTCTGCTAACTCTGGCG                                          <  SRR4302533.5103534/94‑1 (MQ=60)

                                                    AAACGACCCGTCTCGGCCAGTCTGGCCCGGGCC‑TTTTTTACATTGTGCTGCTGTCGATTCTTTCCACGGGTATCGCTCTGCTAACTCTGGCGAG                                        <  SRR4302533.387992/94‑1 (MQ=60)

                                                     AACGACCCGTCTCGGCCAGTCTGGCCCGGGCC‑TTTTTTACATTGTGCTGCTGTCGATTCTTTCCACGGGTATCGCTCTGCTAACTCTGGCGAGC                                       <  SRR4302533.1224835/94‑1 (MQ=60)

                                                       CGACCCGTCTCGGCCAGTCTGGCCCGGGCC‑TTTTTTACATTGTGCTGCTGTCGATTCTTTCCACGGGTATCGCTCTGCTAACTCTGGCGAGCAG                                     >  SRR4302533.4945725/1‑94 (MQ=60)

                                                       CGACCCGTCTCGGCCAGTCTGGCCCGGGCC‑TTTTTTACATTGTGCTGCTGTCGATTCTTTCCACGGGTATCGCTCTGCTAACTCTGGCGAGCAG                                     >  SRR4302533.5527344/1‑94 (MQ=60)

                                                       CGACCCGTCTCGGCCAGTCTGGCCCGGGCC‑TTTTTTACATTGTGCTGCTGTCGATTCTTTCCACGGGTATCGCTCTGCTAACTCTGGCGAGCAG                                     >  SRR4302533.1556322/1‑94 (MQ=60)

                                                         ACCCGTCTCGGCCAGTCTGGCCCGGGCC‑TTTTTTACATTGTGCTGCTGTCGATTCTTTCCACGGGTATCGCTCTGCTAACTCTGGCGAGCAGTT                                   >  SRR4302533.6080790/1‑94 (MQ=60)

                                                         ACCCGTCTCGGCCAGTCTGGCCCGGGCC‑TTTTTTACATTGTGCTGCTGTCGATTCTTTCCACGGGTATCGCTCTGCTAACTCTGGCGAGCAGTT                                   >  SRR4302533.4049533/1‑94 (MQ=60)

                                                         ACCCGTCTCGGCCAGTCTGGCCCGGGCC‑TTTTTTACATTGTGCTGCTGTCGATTCTTTCCACGGGTATCGCTCTGCTAACTCTGGCGAGCAGTT                                   >  SRR4302533.3192461/1‑94 (MQ=60)

                                                          CCCGTCTCGGCCAGTCTGGCCCGGGCC‑TTTTTTACATTGTGCTGCTGTCGATTCTTTCCACGGGTATCGCTCTGCTAACTCTGGCGAGCAGTTT                                  <  SRR4302533.5203352/94‑1 (MQ=60)

                                                          CCCGTCTCGGCCAGTCTGGCCCGGGCC‑TTTTTTACATTGTGCTGCTGTCGATTCTTTCCACGGGTATCGCTCTGCTAACTCTGGCGAGCAGTTT                                  <  SRR4302533.184029/94‑1 (MQ=60)

                                                          CCCGTCTCGGCCAGTCTGGCCCGGGCC‑TTTTTTACATTGTGCTGCTGTCGATTCTTTCCACGGGTATCGCTCTGCTAACTCTGGCGAGCAGTTT                                  <  SRR4302533.6218651/94‑1 (MQ=60)

                                                          CCCGTCTCGGCCAGTCTGGCCCGGGCC‑TTTTTTACATTGTGCTGCTGTCGAGTCTTTCCACGGGTATCGCTCTGCTATCTCTGGCGAGCAGTTT                                  >  SRR4302533.2904535/1‑94 (MQ=60)

                                                           CCGTCTCGGCCAGTCTGGCCCGGGCC‑TTTTTTACATTGTGCTGCTGTCGATTCTTTCCACGGGTATCGCTCTGCTAACTCTGGCGAGCAGTTTG                                 <  SRR4302533.2629024/94‑1 (MQ=60)

                                                           CCGTCTCGGCCAGTCTGGCCCGGGCC‑TTTTTTACATTGTGCTGCTGTCGATTCTTTCCACGGGTATCGCTCTGCTAACTCTGGCGAGCAGTTTG                                 <  SRR4302533.34211/94‑1 (MQ=60)

                                                            CGTCTCGGCCAGTCTGGCCCGGGCC‑TTTTTTACATTGTGCTGCTGTCGATTCTTTCCACGGGTATCGCTCTGCTAACTCTGGCGAGCAGTTTGC                                >  SRR4302533.4797304/1‑94 (MQ=60)

                                                            CGTCTCGGCCAGTCTGGCCCGGGCC‑TTTTTTACATTGTGCTGCTGTCGATTCTTTCCACGGGTATCGCTCTGCTAACTCTGGCGAGCAGTTTGC                                <  SRR4302533.2500763/94‑1 (MQ=60)

                                                             GTCTCGGCCAGTCTGGCCCGGGCC‑TTTTTTACATTGTGCTGCTGTCGATTCTTTCCACGGGTATCGCTCTGCTAACTCTGGCGAGCAGTTTGCG                               <  SRR4302533.1497681/94‑1 (MQ=60)

                                                             GTCTCGGCCAGTCTGGCCCGGGCC‑TTTTTTACATTGTGCTGCTGTCGATTCTTTCCACGGGTATCGCTCTGCTAACTCTGGCGAGCAGTTTGCG                               <  SRR4302533.1235377/94‑1 (MQ=60)

                                                             GTCTCGGCCAGTCTGGCCCGGGCC‑TTTTTTACATTGTGCTGCTGTCGATTCTTTCCACGGGTATCGCTCTGCTAACTCTGGCGAGCAGTTTGCG                               >  SRR4302533.727084/1‑94 (MQ=60)

                                                             GTCTCGGCCAGTCTGGCCCGGGCC‑TTTTTTACATTGTGCTGCTGTCGATTCTTTCCACGGGTATCGCTCTGCTAACTCTGGCGAGCAGTTTGCG                               <  SRR4302533.2848522/94‑1 (MQ=60)

                                                             GTCTCGGCCAGTCTGGCCCGGGCC‑TTTTTTACATTGTGCTGCTGTCGATTCTTTCCACGGGTATCGCTCTGCTAACTCTGGCGAGCAGTTTGCG                               >  SRR4302533.436006/1‑94 (MQ=60)

                                                             GTCTCGGCCAGTCTGGCCCGGGCC‑TTTTTTACATTGTGCTGCTGTCGATTCTTTCCACGGGTATCGCTCTGCTAACTCTGGCGAGCAGTTTGCG                               >  SRR4302533.500871/1‑94 (MQ=60)

                                                             GTCTCGGCCAGTCTGGCCCGGGCC‑TTTTTTACATTGTGCTGCTGTCGATTCTTTCCACGGGTATCGCTCTGCTAACTCTGGCGAGCAGTTTGCG                               <  SRR4302533.3926718/94‑1 (MQ=60)

                                                             GTCTCGGCCAGTCTGGCCCGGGCC‑TTTTTTACATTGTGCTGCTGTCGATTCTTTCCACGGGAATCGCTCTGCTAACTCTGGCGAGCAGcatgag                               >  SRR4302533.4182938/1‑88 (MQ=60)

                                                               CTCGGCCAGTCTGGCCCGGGCC‑TTTTTTACATTGTGCTGCTGTCGATTCTTTCCACGGGTATCGCTCTGCTAACTCTGGCGAGCAGTTTGCGCG                             <  SRR4302533.484662/94‑1 (MQ=60)

                                                               CTCGGCCAGTCTGGCCCGGGCC‑TTTTTTACATTGTGCTGCTGTCGATTCTTTCCACGGGTATCGCTCTGCTAACTCTGGCGAGCAGTTTGCGCG                             <  SRR4302533.2362698/94‑1 (MQ=60)

                                                                TCGGCCAGTCTGGCCCGGGCC‑TTTTTTACATTGTGCTGCTGTCGATTCTTTCCACGGGTATCGCTCTGCTAACTCTGGCGAGCAGTTTGCGCGA                            >  SRR4302533.416398/1‑94 (MQ=60)

                                                                 CGGCCAGTCTGGCCCGGGCC‑TTTTTTACATTGTGCTGCTGTCGATTCTTTCCACGGGTATCGCTCTGCTAACTCTGGCGAGCAGTTTGCGCGAC                           <  SRR4302533.1676131/94‑1 (MQ=60)

                                                                 CGGCCAGTCTGGCCCGGGCC‑TTTTTTACATTGTGCTGCTGTCGATTCTTTCCACGGGTATCGCTCTGCTAACTCTGGCGAGCAGTTTGCGCGAC                           >  SRR4302533.629460/1‑94 (MQ=60)

                                                                 CGGCCAGTCTGGCCCGGGCC‑TTTTTTACATTGTGCTGCTGTCGATTCTTTCCACGGGTATCGCTCTGCTAACTCTGGCGAGCAGTTTGCGCGAC                           >  SRR4302533.5715538/1‑94 (MQ=60)

                                                                  GGCCAGTCTGGCCCGGGCC‑TTTTTTACATTGTGCTGCTGTCGATTCTTTCCACGGGTATCGCTCTGCTAACTCTGGCGAGCAGTTTGCGCGACG                          >  SRR4302533.5055301/1‑94 (MQ=60)

                                                                  GGCCAGTCTGGCCCGGGCC‑TTTTTTACATTGTGCTGCTGTCGATTCTTTCCACGGGTATCGCTCTGCTAACTCTGGCGAGCAGTTTGCGCGACG                          >  SRR4302533.5407204/1‑94 (MQ=60)

                                                                    CCAGTCTGGCCCGGGCC‑TTTTTTACATTGTGCTGCTGTCGATTCTTTCCACGGGTATCGCTCTGCTAACTCTGGCGAGCAGTTTGCGCGACGCT                        <  SRR4302533.1419939/94‑1 (MQ=60)

                                                                      AGTCTGGCCCGGGCC‑TTTTTTACATTGTGCTGCTGTCGATTCTTTCCACGGGTATCGCTCTGCTAACTCTGGCGAGCAGTTTGCGCGACGCTGA                      >  SRR4302533.5624445/1‑94 (MQ=60)

                                                                       GTCTGGCCCGGGCC‑TTTTTTACATTGTGCTGCTGTCGATTCTTTCCACGGGTATCGCTCTGCTAACTCTGGCGAGCAGTTTGCGCGACGCTGAG                     <  SRR4302533.5319317/94‑1 (MQ=60)

                                                                       GTCTGGCCCGGGCC‑TTTTTTACATTGTGCTGCTGTCGATTCTTTCCACGGGTATCGCTCTGCTAACTCTGGCGAGCAGTTTGCGCGACGCTGAG                     >  SRR4302533.3759320/1‑94 (MQ=60)

                                                                       GTCTGGCCCGGGCC‑TTTTTTACATTGTGCTGCTGTCGATTCTTTCCACGGGTATCGCTCTGCTAACTCTGGCGAGCAGTTTGCGCGACGCTGAG                     >  SRR4302533.5354232/1‑94 (MQ=60)

                                                                       GTCTGGCCCGGGCC‑TTTTTTACATTGTGCTGCTGTCGATTCTTTCCACGGGTATCGCTCTGCTAACTCTGGCGAGCAGTTTGCGCGACGCTGAG                     >  SRR4302533.5010812/1‑94 (MQ=60)

                                                                           GGCCCGGGCC‑TTTTTTACATTGTGCTGCTGTCGATTCTTTCCACGGGTATCGCTCTGCTAACTCTGGCGAGCAGTTTGCGCGACGCTGAGGCTA                 >  SRR4302533.1058325/1‑94 (MQ=60)

                                                                            GCCCGGGCC‑TTTTTTACATTGTGCTGCTGTCGATTCTTTCCACGGGTATCGCTCTGCTAACTCTGGCGAGCAGTTTGCGCGACGCTGAGGCTAT                <  SRR4302533.354952/94‑1 (MQ=60)

                                                                             CCCGGGCC‑TTTTTTACATTGTGCTGCTGTCGATTCTTTCCACGGGTATCGCTCTGCTAACTCTGGCGAGCAGTTTGCGCGACGCTGAGGCTATC               >  SRR4302533.1795911/1‑94 (MQ=60)

                                                                              CCGGGCC‑TTTTTTACATTGTGCTGCTGTCGATTCTTTCCACGGGTATCGCTCTGCTAACTCTGGCGAGCAGTTTGCGCGACGCTGAGGCTATCA              <  SRR4302533.4179617/94‑1 (MQ=60)

                                                                              CCGGGCC‑TTTTTTACATTGTGCTGCTGTCGATTCTTTCCACGGGTATCGCTCTGCTAACTCTGGCGAGCAGTTTGCGCGACGCTGAGGCTATCA              <  SRR4302533.2467882/94‑1 (MQ=60)

                                                                              CCGGGCC‑TTTTTTACATTGTGCTGCTGTCGATTCTTTCCACGGGTATCGCTCTGCTAACTCTGGCGAGCAGTTTGCGCGACGCTGAGGCTATCA              >  SRR4302533.2693766/1‑94 (MQ=60)

                                                                                GGGCC‑TTTTTTACATTGTGCTGCTGTCGATTCTTTCCACGGGTATCGCTCTGCTAACTCTGGCGAGCAGTTTGCGCGACGCTGAGGCTATCAAT            <  SRR4302533.868278/94‑1 (MQ=60)

                                                                                GGGCC‑TTTTTTACATTGTGCTGCTGTCGATTCTTTCCACGGGTATCGCTCTGCTAACTCTGGCGAGCAGTTTGCGCGACGCTGAGGCTATCAAT            >  SRR4302533.2038823/1‑94 (MQ=60)

                                                                                GGGCC‑TTTTTTACATTGTGCTGCTGTCGATTCTTTCCACGGGTATCGCTCTGCTAACTCTGGCGAGCAGTTTGCGCGACGCTGAGGCTATCAAT            >  SRR4302533.1796376/1‑94 (MQ=60)

                                                                                GGGCC‑TTTTTTACATTGTGCTGCTGTCGATTCTTTCCACGGGTATCGCTCTGCTAACTCTGGCGAGCAGTTTGCGCGACGCTGAGGCTATCAAT            <  SRR4302533.3607074/94‑1 (MQ=60)

                                                                                GGGCC‑TTTTTTACATTGTGCTGCTGTCGATTCTTTCCACGGGTATCGCTCTGCTAACTCTGGCGAGCAGTTTGCGCGACGCTGAGGCTATCAAT            >  SRR4302533.5538221/1‑94 (MQ=60)

                                                                                 GGCC‑TTTTTTACATTGTGCTGCTGTCGATTCTTTCCACGGGTATCGCTCTGCTAACTCTGGCGAGCAGTTTGCGCGACGCTGAGGCTATCAATT           >  SRR4302533.3764405/1‑94 (MQ=60)

                                                                                 GGCC‑TTTTTTACATTGTGCTGCTGTCGATTCTTTCCACGGGTATCGCTCTGCTAACTCTGGCGAGCAGTTTGCGCGACGCTGAGGCTATCAATA           >  SRR4302533.4346817/1‑94 (MQ=60)

                                                                                 GGCC‑TTTTTTACATTGTGCTGCTGTCGATTCTTTCCACGGGTATCGCTCTGCTAACTCTGGCGAGCAGTTTGCGCGACGCTGAGGCTATCAATA           >  SRR4302533.2116428/1‑94 (MQ=60)

                                                                                  GCC‑TTTTTTACATTGTGCTGCTGTCGATTCTTTCCACGGGTATCGCTCTGCTAACTCTGGCGAGCAGTTTGCGCGACGCTGAGGCTATCAATAT          >  SRR4302533.6250759/1‑94 (MQ=60)

                                                                                  GCC‑TTTTTTACATTGTGCTGCTGTCGATTCTTTCCACGGGTATCGCTCTGCTAACTCTGGCGAGCAGTTTGCGCGACGCTGAGGCTATCAATAT          >  SRR4302533.3914774/1‑94 (MQ=60)

                                                                                    CCTTTTTTACATTGTGCTGCTGTCGATTCTTTCCACGGGTATCGCTCTGCTAACTCTGGCGAGCAGTTTGCGCGACGCTGAGGCTATCAATATT         >  SRR4302533.2148320/1‑94 (MQ=60)

                                                                                     CTTTTTTACATTGTGCTGCTGTCGATTCTTTCCACGGGTATCGCTCTGCTAACTCTGGCGAGCAGTTTGCGCGACGCTGAGGCTATCAATATTG        >  SRR4302533.4723838/1‑94 (MQ=60)

                                                                                     CTTTTTTACATTGTGCTGCTGTCGATTCTTTCCACGGGTATCGCTCTGCTAACTCTGGCGAGCAGTTTGCGCGACGCTGAGGCTATCAATATTG        >  SRR4302533.1291394/1‑94 (MQ=60)

                                                                                     CTTTTTTACATTGTGCTGCTGTCGATTCTTTCCACGGGTATCGCTCTGCTAACTCTGGCGAGCAGTTTGCGCGACGCTGAGGCTATCAATATTG        <  SRR4302533.5083618/94‑1 (MQ=60)

                                                                                     CTTTTTTACATTGTGCTGCTGTCGATTCTTTCCACGGGTATCGCTCTGCTAACTCTGGCGAGCAGTTTGCGCGACGCTGAGGCTATCAATATTG        <  SRR4302533.5969191/94‑1 (MQ=60)

                                                                                     CTTTTTTACATTGTGCTGCTGTCGATTCTTTCCACGGGTATCGCTCTGCTAACTCTGGCGAGCAGTTTGCGCGACGCTGAGGCTATCAATATTG        >  SRR4302533.5497416/1‑94 (MQ=60)

                                                                                     CTTTTTTACATTGTGCTGCTGTCGATTCTTTCCACGGGTATCGCTCTGCTAACTCTGGCGAGCAGTTTGCGCGACGCTGAGGCTATCAATATTG        >  SRR4302533.3740723/1‑94 (MQ=60)

                                                                                     CTTTTTTACATTGTGCTGCTGTCGATTCTTTCCACGGGTATCGCTCTGCTAACTCTGGCGAGCAGCTTGCGCGACGCTGAGGCTATCAATATTG        >  SRR4302533.2308486/1‑94 (MQ=60)

                                                                                       TTTTTACATTGTGCTGCTGTCGATTCTTTCCACGGGTATCGCTCTGCTAACTCTGGCGAGCAGTTTGCGCGACGCTGAGGCTATCAATATTGCC      <  SRR4302533.3621006/94‑1 (MQ=60)

                                                                                       TTTTTACATTGTGCTGCTGTCGATTCTTTCCACGGGTATCGCTCTGCTAACTCTGGCAAGCAGTTTGCGCGACGCTGAGGCTATCAATATTGCC      <  SRR4302533.4595984/94‑1 (MQ=60)

                                                                                        TTTTACATTGTGCTGCTGTCGATTCTTTCCACGGGTATCGCTCTGCTAACTCTGGCGAGCAGTTTGCGCGACGCTGAGGCTATCAATATTGCCG     >  SRR4302533.3510218/1‑94 (MQ=60)

                                                                                          TTACATTGTGCTACTGTCGATTCTTTCCACGGGTATCGCTCTGCTAACTCTGGCGAGCAGTTTGCGCGACGCTGAGGCTATCAATATTGCCGGA   >  SRR4302533.2391232/1‑94 (MQ=60)

                                                                                           TACATTGTGCTGCTGTCGATTCTTTCCACGGGTATCGCTCTGCTAACTCTGGCGAGCAGTTTGCGCGACGCTGAGGCTATCAATATTGCCGGAT  <  SRR4302533.5123124/94‑1 (MQ=60)

CAGCGAACTGGAACATTAATGATTTTTTGTGGAGAAGACGCGTGTGATTGTTAAACGACCCGTCTCGGCCAGTCTGGCCCGGGCCTTTTTTTACATTGTGCTGCTGTCGATTCTTTCCACGGGTATCGCTCTGCTAACTCTGGCGAGCAGTTTGCGCGACGCTGAGGCTATCAATATTGCCGGAT  >  NC_000913/2583708‑2583892

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 7 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`