Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,766,427 2 bp→AC coding (697‑698/864 nt) b2632 → putative GTP‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,766,4270TA99.5% 732.2 / NA 205W233T (TGG→ACG) b2632putative GTP‑binding protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base A (108/96);  minor base G (0/1);  total (108/97)
Fisher's exact test for biased strand distribution p-value = 4.73e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*NC_0009132,766,4280GC100.0% 646.8 / NA 205W233T (TGG→ACG) b2632putative GTP‑binding protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (108/97);  total (108/97)

Only 100 of 208 total aligned reads displayed.

AGGGTGATGGCAGAGCGGATGATTAAGTGTCTGCCGCGTGAGCCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGGACATGGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGATACCATACTCGATACGGTAAGCACCTTTCCCCTTATTC  >  NC_000913/2766340‑2766511
                                                                                       ||                                                                                   
aGGGTGATGGCAGAGCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTa                                                                                 <  1:4032107/93‑1 (MQ=25)
aGGGTGATGGCAGAGCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTa                                                                                 >  2:211718/1‑93 (MQ=25)
  ggTGATGGCAGAGCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGc                                                                               >  2:1877592/1‑93 (MQ=21)
   gTGATGGCAGAGCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGcc                                                                              <  1:3940248/93‑1 (MQ=21)
    tGATGGCAGAGCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGccc                                                                             >  2:1940208/1‑93 (MQ=25)
    tGATGGCAGAGCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGccc                                                                             <  2:284863/93‑1 (MQ=25)
    tGATGGCAGAGCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGccc                                                                             >  2:5274495/1‑93 (MQ=21)
    tGATGGCAGAGCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGccc                                                                             <  1:2174391/93‑1 (MQ=25)
     gATGGCAGAGCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCg                                                                            >  1:2898638/1‑93 (MQ=25)
      aTGGCAGAGCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCgg                                                                           >  1:2962268/1‑93 (MQ=25)
       tGNCAGNGCGGATGATTAAGTNNCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCggg                                                                          <  1:2307364/93‑1 (MQ=21)
       tGGCAGAGCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCggg                                                                          <  1:4487869/93‑1 (MQ=21)
       tGGCAGAGCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCggg                                                                          >  2:3889716/1‑93 (MQ=21)
        ggCAGAGCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGa                                                                         <  1:4209537/93‑1 (MQ=21)
           agagCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCa                                                                      <  1:2335107/93‑1 (MQ=37)
            gagCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAg                                                                     >  2:1056542/1‑93 (MQ=25)
            gagCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAg                                                                     >  2:5807482/1‑93 (MQ=21)
              gCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGc                                                                   >  2:4451851/1‑93 (MQ=21)
               gggATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCa                                                                  <  1:1682654/92‑1 (MQ=255)
                ggATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCAc                                                                 >  1:6221589/1‑93 (MQ=255)
                ggATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCAc                                                                 >  2:2712445/1‑93 (MQ=255)
                ggATGATTAAGTGTCGGCCGCGTGAGGCCAGCAGCCTGGTAGTAGCGCTACTGCAACATCCCTCTCGAACAACGGTAGCCCGGGAGCAGGCAc                                                                 <  2:5003746/93‑1 (MQ=255)
                 gatgatTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACg                                                                >  2:3222386/1‑93 (MQ=255)
                 gatgatTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACg                                                                >  2:4510455/1‑93 (MQ=255)
                    gatTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGa                                                             <  1:1456986/93‑1 (MQ=255)
                    gatTAAGTGTCTGCCGCGTGAGGCCAGCAGCCAGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGa                                                             <  2:2504144/93‑1 (MQ=255)
                     atTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGAc                                                            >  1:824822/1‑93 (MQ=255)
                      ttAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACg                                                           >  2:2921888/1‑93 (MQ=255)
                       tAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGa                                                          <  1:4928484/93‑1 (MQ=255)
                        aaGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGAt                                                         >  2:3398346/1‑93 (MQ=255)
                           tgtCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTc                                                      >  1:2714957/1‑93 (MQ=255)
                            gtCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAACAGGCACGTGACGATTTCg                                                     <  2:6132819/93‑1 (MQ=255)
                             tcggCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCgg                                                    <  2:4919214/90‑1 (MQ=255)
                             tCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCgg                                                    <  2:2948266/93‑1 (MQ=255)
                             gcTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCgg                                                    <  2:1744534/92‑1 (MQ=255)
                                 ccGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGaa                                                <  2:2633104/93‑1 (MQ=255)
                                  cgcgTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGaaa                                               >  1:1092772/1‑93 (MQ=255)
                                   gcgTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAATAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAAc                                              <  2:1404538/93‑1 (MQ=255)
                                   gcgTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAAc                                              >  2:3810491/1‑93 (MQ=255)
                                   gcgTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAAc                                              >  2:6197881/1‑93 (MQ=255)
                                   gcgTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAAc                                              >  1:6154247/1‑93 (MQ=255)
                                    cgTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAAcc                                             >  1:4423070/1‑93 (MQ=255)
                                     gTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCg                                            >  1:750504/1‑93 (MQ=255)
                                      tGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGt                                           >  1:5200277/1‑93 (MQ=255)
                                       gAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTa                                          <  1:1583748/93‑1 (MQ=255)
                                       gAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTa                                          <  2:3289101/93‑1 (MQ=255)
                                        aggccAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAg                                         <  2:3477865/90‑1 (MQ=255)
                                        aggccAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAg                                         <  2:3070888/90‑1 (MQ=255)
                                        aggccAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAg                                         >  1:1390464/4‑93 (MQ=255)
                                        aggccAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAg                                         >  2:4205930/4‑93 (MQ=255)
                                        aggccAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAg                                         <  1:1863350/90‑1 (MQ=255)
                                         ggccAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAgg                                        <  1:3651634/91‑2 (MQ=255)
                                          gccAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAggt                                       <  2:594603/92‑3 (MQ=255)
                                            cagcagCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAggtgc                                     >  1:5473084/1‑89 (MQ=255)
                                                agcCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATa                                 <  1:4158922/93‑1 (MQ=255)
                                                 gcCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATAc                                <  2:4114997/93‑1 (MQ=255)
                                                 gcCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATAc                                <  1:1181307/93‑1 (MQ=255)
                                                  cccGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACt                               >  1:3080200/1‑93 (MQ=255)
                                                   ccGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTc                              >  1:2473326/1‑93 (MQ=255)
                                                   ccGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTc                              <  2:4477722/93‑1 (MQ=255)
                                                   ccGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTc                              >  1:3479040/1‑93 (MQ=255)
                                                    cGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCg                             >  2:2165763/1‑93 (MQ=255)
                                                    cGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCg                             >  2:4305163/1‑93 (MQ=255)
                                                     ggTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCATGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGa                            <  2:1022866/93‑1 (MQ=255)
                                                     ggTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGa                            >  2:5652665/1‑93 (MQ=255)
                                                      gtagtaGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGAt                           <  2:1626838/93‑1 (MQ=255)
                                                      gtagtaGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGAt                           >  1:1989869/1‑93 (MQ=255)
                                                       tagtagCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATa                          >  2:5773134/1‑93 (MQ=255)
                                                        agtagCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATAc                         <  1:3222386/93‑1 (MQ=255)
                                                        agtagCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATAc                         <  1:4611489/93‑1 (MQ=255)
                                                         gtagCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACg                        <  1:2426865/93‑1 (MQ=255)
                                                         gtagCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACg                        >  2:3600077/1‑93 (MQ=255)
                                                           agCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGt                      >  1:561705/1‑93 (MQ=255)
                                                            gcgcTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTa                     >  2:108008/1‑93 (MQ=255)
                                                             cgcTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTaa                    <  2:1903890/93‑1 (MQ=255)
                                                             cgcTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTaa                    <  2:2326839/93‑1 (MQ=255)
                                                             cgcTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTaa                    >  1:3968969/1‑93 (MQ=255)
                                                             cgcTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGGGCCGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTaa                    <  1:2147605/93‑1 (MQ=255)
                                                              tcTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAg                   <  2:3969859/92‑1 (MQ=255)
                                                               cTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGc                  <  2:2667442/93‑1 (MQ=255)
                                                               cTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGc                  >  2:1183252/1‑93 (MQ=255)
                                                               cTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGc                  >  2:4553525/1‑93 (MQ=255)
                                                               cTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGc                  >  1:4276966/1‑93 (MQ=255)
                                                                tACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCa                 >  2:5525184/1‑93 (MQ=255)
                                                                  cTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCAcc               >  2:2291826/1‑93 (MQ=255)
                                                                   tGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCt              <  1:5950320/93‑1 (MQ=255)
                                                                   tGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCt              >  1:5024920/1‑93 (MQ=255)
                                                                    gCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCtt             >  1:311471/1‑93 (MQ=255)
                                                                     cAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCttt            >  1:5911910/1‑93 (MQ=255)
                                                                      aaCATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTc           >  2:4830411/1‑93 (MQ=255)
                                                                      aaCATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTc           >  1:2500239/1‑93 (MQ=255)
                                                                      aaCATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTc           >  1:3321861/1‑93 (MQ=255)
                                                                       aCATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTcc          >  1:2456371/1‑93 (MQ=255)
                                                                        cATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCTATACGGTAAGCACCTTTccc         >  2:1380022/1‑93 (MQ=255)
                                                                        cATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTccc         <  1:5316215/93‑1 (MQ=255)
                                                                        cATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTccc         >  2:398984/1‑93 (MQ=255)
                                                                         aTCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTcccc        <  1:874755/93‑1 (MQ=255)
                                                                          tCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCt       >  2:4565118/1‑93 (MQ=255)
                                                                            ccTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTa     <  2:5627425/93‑1 (MQ=255)
                                                                            ccTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTa     <  2:129773/93‑1 (MQ=255)
                                                                                       ||                                                                                   
AGGGTGATGGCAGAGCGGATGATTAAGTGTCTGCCGCGTGAGCCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGGACATGGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGATACCATACTCGATACGGTAAGCACCTTTCCCCTTATTC  >  NC_000913/2766340‑2766511

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AGGGTGATGGCAGAGCGGATGATTAAGTGTCTGCCGCGTGAGCCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGGACATGGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGATACCATACTCGATACGGTAAGCACCTTTCCCCTTATT  >  NC_000913/2766340‑2766510
                                                                                       |                                                                                   
AGGGTGATGGCAGAGCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAG                                                                               <  SRR4302533.2553137/94‑1 (MQ=60)
AGGGTGATGGCAGAGCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAG                                                                               <  SRR4302533.4056653/94‑1 (MQ=60)
   GTGATGGCAGAGCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCC                                                                            <  SRR4302533.3964292/94‑1 (MQ=60)
    TGATGGCAGAGCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCG                                                                           >  SRR4302533.2916452/1‑94 (MQ=60)
    TGATGGCAGAGCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCG                                                                           <  SRR4302533.2187790/94‑1 (MQ=60)
    TGATGGCAGAGCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCG                                                                           <  SRR4302533.717966/94‑1 (MQ=60)
     GATGGCAGAGCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGG                                                                          >  SRR4302533.2980378/1‑94 (MQ=60)
      ATGGCAGAGCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGG                                                                         >  SRR4302533.30129/1‑94 (MQ=60)
      ATGGCAGAGCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGG                                                                         <  SRR4302533.811554/94‑1 (MQ=60)
       TGNCAGNGCGGATGATTAAGTNNCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGA                                                                        <  SRR4302533.2321658/94‑1 (MQ=60)
       TGGCAGAGCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGA                                                                        <  SRR4302533.4515037/94‑1 (MQ=60)
        GGCAGAGCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAG                                                                       >  SRR4302533.2844094/1‑94 (MQ=60)
        GGCAGAGCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAG                                                                       <  SRR4302533.4235002/94‑1 (MQ=60)
           AGAGCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAG                                                                    >  SRR4302533.4205505/1‑94 (MQ=60)
           AGAGCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAG                                                                    <  SRR4302533.2349562/94‑1 (MQ=60)
               GGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCAC                                                                <  SRR4302533.1692846/94‑1 (MQ=60)
               CGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCAC                                                                >  SRR4302533.6258880/1‑94 (MQ=60)
                  ATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTG                                                             >  SRR4302533.5297247/1‑94 (MQ=60)
                  ATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTG                                                             <  SRR4302533.2195509/94‑1 (MQ=60)
                    GATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGAC                                                           >  SRR4302533.3176589/1‑94 (MQ=60)
                    GATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGAC                                                           <  SRR4302533.1465772/94‑1 (MQ=60)
                    GATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGAC                                                           >  SRR4302533.829662/1‑94 (MQ=60)
                       TAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGAT                                                        <  SRR4302533.4958329/94‑1 (MQ=60)
                        AAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATT                                                       >  SRR4302533.4389047/1‑94 (MQ=60)
                         AGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTT                                                      <  SRR4302533.2173424/94‑1 (MQ=60)
                          GTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTC                                                     >  SRR4302533.2731626/1‑94 (MQ=60)
                           TGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCG                                                    >  SRR4302533.470670/1‑94 (MQ=60)
                            GTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGG                                                   >  SRR4302533.1104169/1‑94 (MQ=60)
                                GCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAA                                               >  SRR4302533.4035727/1‑94 (MQ=60)
                                GCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAA                                               <  SRR4302533.1245828/94‑1 (MQ=60)
                                 CCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAA                                              <  SRR4302533.2965387/94‑1 (MQ=60)
                                 CCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAA                                              >  SRR4302533.1099234/1‑94 (MQ=60)
                                  CGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAAC                                             >  SRR4302533.6191278/1‑94 (MQ=60)
                                   GCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACC                                            >  SRR4302533.537467/1‑94 (MQ=60)
                                   GCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACC                                            <  SRR4302533.6102399/94‑1 (MQ=60)
                                   GCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACC                                            >  SRR4302533.4449810/1‑94 (MQ=60)
                                    CGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCG                                           <  SRR4302533.141549/94‑1 (MQ=60)
                                    CGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCG                                           <  SRR4302533.1852156/94‑1 (MQ=60)
                                    CGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCG                                           >  SRR4302533.754922/1‑94 (MQ=60)
                                     GTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGT                                          >  SRR4302533.5231626/1‑94 (MQ=60)
                                     GTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGT                                          >  SRR4302533.3620073/1‑94 (MQ=60)
                                       GAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAG                                        >  SRR4302533.1955356/1‑94 (MQ=60)
                                       GAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAG                                        >  SRR4302533.1398839/1‑94 (MQ=60)
                                       GAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAG                                        <  SRR4302533.1593292/94‑1 (MQ=60)
                                       GAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAG                                        <  SRR4302533.2339281/94‑1 (MQ=60)
                                       GAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAG                                        <  SRR4302533.2470693/94‑1 (MQ=60)
                                        AGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGG                                       <  SRR4302533.1874717/94‑1 (MQ=60)
                                         GGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGT                                      <  SRR4302533.3674085/94‑1 (MQ=60)
                                          GCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGgtg                                     >  SRR4302533.5080890/1‑91 (MQ=60)
                                           CCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGgtgc                                    >  SRR4302533.5506071/1‑90 (MQ=60)
                                            CAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGgtgcc                                   <  SRR4302533.3105444/94‑6 (MQ=60)
                                             AGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCA                                  >  SRR4302533.2392840/1‑94 (MQ=60)
                                                AGCGCGGTAGTGGCGCTACTGCAAAATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATAC                               <  SRR4302533.5515957/94‑1 (MQ=60)
                                                AGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATAC                               >  SRR4302533.5362490/1‑94 (MQ=60)
                                                AGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATAC                               <  SRR4302533.4184126/94‑1 (MQ=60)
                                                 GCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACT                              <  SRR4302533.500185/94‑1 (MQ=60)
                                                 GCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACT                              >  SRR4302533.3099098/1‑94 (MQ=60)
                                                 GCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACT                              <  SRR4302533.1188403/94‑1 (MQ=60)
                                                  CCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTC                             >  SRR4302533.3500506/1‑94 (MQ=60)
                                                  CCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTC                             >  SRR4302533.879250/1‑94 (MQ=60)
                                                  CCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTC                             <  SRR4302533.4498951/94‑1 (MQ=60)
                                                  CCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTC                             <  SRR4302533.4601523/94‑1 (MQ=60)
                                                  CCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTC                             >  SRR4302533.2488577/1‑94 (MQ=60)
                                                   CCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCG                            >  SRR4302533.3455315/1‑94 (MQ=60)
                                                   CCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCG                            >  SRR4302533.1598764/1‑94 (MQ=60)
                                                    CGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGA                           >  SRR4302533.1578914/1‑94 (MQ=60)
                                                     GGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGAT                          >  SRR4302533.2508237/1‑94 (MQ=60)
                                                     GGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGAT                          >  SRR4302533.2002051/1‑94 (MQ=60)
                                                      GTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATA                         >  SRR4302533.3959190/1‑94 (MQ=60)
                                                       TAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATAC                        <  SRR4302533.5967563/94‑1 (MQ=60)
                                                       TAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATAC                        <  SRR4302533.2583283/94‑1 (MQ=60)
                                                        AGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACG                       >  SRR4302533.4148461/1‑94 (MQ=60)
                                                        AGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACG                       <  SRR4302533.3242258/94‑1 (MQ=60)
                                                        AGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACG                       >  SRR4302533.32874/1‑94 (MQ=60)
                                                        AGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACG                       <  SRR4302533.4639429/94‑1 (MQ=60)
                                                        AGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACG                       <  SRR4302533.3734424/94‑1 (MQ=60)
                                                        AGTAGAGATACTGCAACATCCCTTTCGAACAGCGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACG                       <  SRR4302533.585470/94‑1 (MQ=60)
                                                         GTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGG                      <  SRR4302533.2441870/94‑1 (MQ=60)
                                                         GTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGG                      <  SRR4302533.248047/94‑1 (MQ=60)
                                                          TAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGT                     >  SRR4302533.565037/1‑94 (MQ=60)
                                                            GCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAA                   >  SRR4302533.3993144/1‑94 (MQ=60)
                                                             CGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGGGCCGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAG                  <  SRR4302533.2160833/94‑1 (MQ=60)
                                                              GCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGC                 >  SRR4302533.4302687/1‑94 (MQ=60)
                                                               CTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCA                >  SRR4302533.908008/1‑94 (MQ=60)
                                                                  CTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCT             >  SRR4302533.5055348/1‑94 (MQ=60)
                                                                   TGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTT            >  SRR4302533.4302425/1‑94 (MQ=60)
                                                                   TGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTT            <  SRR4302533.5986307/94‑1 (MQ=60)
                                                                   TGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTT            >  SRR4302533.313377/1‑94 (MQ=60)
                                                                    GCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTT           >  SRR4302533.5947633/1‑94 (MQ=60)
                                                                     CAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTC          >  SRR4302533.3342379/1‑94 (MQ=60)
                                                                     CAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTC          >  SRR4302533.2515662/1‑94 (MQ=60)
                                                                      AACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCC         >  SRR4302533.2471531/1‑94 (MQ=60)
                                                                      AACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCC         <  SRR4302533.2088633/94‑1 (MQ=60)
                                                                        CATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCC       <  SRR4302533.5348072/94‑1 (MQ=60)
                                                                         ATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCT      >  SRR4302533.6195952/1‑94 (MQ=60)
                                                                         ATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCT      <  SRR4302533.879890/94‑1 (MQ=60)
                                                                            CCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTAT   <  SRR4302533.2447407/94‑1 (MQ=60)
                                                                             CTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATT  >  SRR4302533.2655511/1‑94 (MQ=60)
                                                                             CTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATT  >  SRR4302533.6054275/1‑94 (MQ=60)
                                                                             CTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATT  >  SRR4302533.1008904/1‑94 (MQ=60)
                                                                             CTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATT  >  SRR4302533.89841/1‑94 (MQ=60)
                                                                                       |                                                                                   
AGGGTGATGGCAGAGCGGATGATTAAGTGTCTGCCGCGTGAGCCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGGACATGGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGATACCATACTCGATACGGTAAGCACCTTTCCCCTTATT  >  NC_000913/2766340‑2766510

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑