Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,960,578 2 bp→CG coding (1266‑1267/1485 nt) gppA ← guanosine pentaphosphatase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,960,5780GC100.0% 864.5 / NA 269L423V (CTC→GTC) gppAguanosine pentaphosphatase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (120/149);  total (120/149)
*NC_0009133,960,5790CG100.0% 868.5 / NA 269R422R (CGG→CGC) gppAguanosine pentaphosphatase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (120/149);  total (120/149)

Only 100 of 279 total aligned reads displayed.

GTAATGTCATCTCTGGCACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGAGCCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTAGCGTCGCCAGCAATTTTTT  >  NC_000913/3960491‑3960665
                                                                                       ||                                                                                      
gTAATGTCATCTCTGGCACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGGGGTGTTTTGCGAcgcgcg                                                                                    >  2:2715599/1‑93 (MQ=255)
gTAATGTCATCTCTGGCACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGAcgcgcg                                                                                    >  2:964492/1‑93 (MQ=255)
   aTGTCATCTCTGGCACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCgcggcg                                                                                 <  2:914873/93‑1 (MQ=255)
   aTGTCATCTCTGGCACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCgcggcg                                                                                 >  1:1999277/1‑93 (MQ=255)
   aTGTCATCTCTGGCACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCgcggcg                                                                                 >  2:5480021/1‑93 (MQ=255)
   aTGTCATCTCTGGCACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCgcggcg                                                                                 <  2:5296649/93‑1 (MQ=255)
   aTGTCATCTCTGGCACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCgcggcg                                                                                 >  2:5349334/1‑93 (MQ=255)
    tGTCATCTCTGGCACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGcggcgg                                                                                >  1:2759260/1‑93 (MQ=255)
    tGTCATCTCTGGCACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGcggcgg                                                                                >  1:1724350/1‑93 (MQ=255)
    tGTCATCTCTGGCACGAGATCGTCACGGCGACGGCTGGCAAAAATGATCGCCAGGCCTAGTAAACGGCAGAGTTGTTCTGCGACGCGcggcgg                                                                                >  2:6235266/1‑93 (MQ=255)
     gTCATCTCTGGCACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGt                                                                               >  1:2589256/1‑93 (MQ=255)
     gTCATCTCTGGCACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGt                                                                               <  2:5030418/93‑1 (MQ=255)
      tCATCTCTGGCACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTa                                                                              <  1:746490/93‑1 (MQ=255)
      tCATCTCTGGCACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGATGTTCTGCGACGCGCGGCGGTa                                                                              >  2:3023723/1‑93 (MQ=255)
       cATCTCTGGCACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTAc                                                                             <  2:3942739/93‑1 (MQ=255)
        aTCTCTGGCACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACg                                                                            <  2:3347491/93‑1 (MQ=255)
         tctcTGGCACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACgg                                                                           >  2:4329106/1‑93 (MQ=255)
         tctcTGGCACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACgg                                                                           >  2:3418979/1‑93 (MQ=255)
          ctctGGCACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGc                                                                          >  2:1246374/1‑93 (MQ=255)
          ctctGGCACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGc                                                                          <  2:6008886/93‑1 (MQ=255)
          ctctGGCACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGc                                                                          <  1:3278592/93‑1 (MQ=255)
           tctGGCACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCa                                                                         >  2:1978487/1‑93 (MQ=255)
            ctGGCACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCAt                                                                        <  1:4295048/93‑1 (MQ=255)
              ggCACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCAttt                                                                      <  1:2369022/93‑1 (MQ=255)
                  cGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCt                                                                  >  1:3037746/1‑93 (MQ=255)
                  cGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCt                                                                  >  1:622375/1‑93 (MQ=255)
                  cGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCt                                                                  >  2:810368/1‑93 (MQ=255)
                   gagaTCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTg                                                                 >  1:5021697/1‑93 (MQ=255)
                    agaTCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGa                                                                <  1:2181954/93‑1 (MQ=255)
                    agaTCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGa                                                                <  1:5777122/93‑1 (MQ=255)
                     gaTCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGccagc                                                               <  2:3477528/93‑5 (MQ=255)
                     gaTCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGAt                                                               <  2:1186326/93‑1 (MQ=255)
                     gaTCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGAt                                                               >  1:5509411/1‑93 (MQ=255)
                      aTCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATg                                                              <  2:5779180/93‑1 (MQ=255)
                        cGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCa                                                            <  2:4273877/93‑1 (MQ=255)
                        cGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCa                                                            <  1:1123926/93‑1 (MQ=255)
                         gTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAg                                                           >  1:3189104/1‑93 (MQ=255)
                          tCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGc                                                          <  2:3665955/93‑1 (MQ=255)
                          tCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGc                                                          <  1:212182/93‑1 (MQ=255)
                           cACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCg                                                         <  1:703234/93‑1 (MQ=255)
                           cACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCg                                                         <  1:6069104/93‑1 (MQ=255)
                            aCGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGa                                                        <  2:1006713/93‑1 (MQ=255)
                            aCGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGa                                                        >  1:918770/1‑93 (MQ=255)
                             cGGCGACGGCTGGCAAAAATGATGTCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGAt                                                       <  2:2136811/93‑1 (MQ=255)
                             cGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGAt                                                       <  1:2681509/93‑1 (MQ=255)
                               gCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATga                                                     >  2:4717884/1‑93 (MQ=255)
                               gCGACGGCTGACAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATga                                                     <  1:3791854/93‑1 (MQ=255)
                                cGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATgag                                                    <  1:5576218/93‑1 (MQ=255)
                                  aCGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATgaggg                                                  >  1:5260597/1‑91 (MQ=255)
                                  aCGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATgagag                                                  >  1:944715/1‑93 (MQ=255)
                                   cGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATgagaga                                                 >  1:5884684/1‑93 (MQ=255)
                                   cGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATgagaga                                                 <  2:5615358/93‑1 (MQ=255)
                                    ggCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGAt                                                <  1:3658103/93‑1 (MQ=255)
                                     gCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATc                                               <  2:4830164/93‑1 (MQ=255)
                                     gCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATc                                               <  1:1015099/93‑1 (MQ=255)
                                       tGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGa                                             <  2:230297/93‑1 (MQ=255)
                                        ggCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGAc                                            <  1:1242652/93‑1 (MQ=255)
                                         gCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGAcc                                           <  1:1494570/93‑1 (MQ=255)
                                          cAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCg                                          >  1:5125222/1‑93 (MQ=255)
                                              aaTGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGAtt                                      >  1:852293/1‑93 (MQ=255)
                                               atgatgGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTa                                     <  2:1380152/93‑1 (MQ=255)
                                                tgatgGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAg                                    >  2:3202312/1‑93 (MQ=255)
                                                  atgGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTc                                  <  2:4108930/93‑1 (MQ=255)
                                                   tgGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCt                                 <  2:5383745/93‑1 (MQ=255)
                                                   gggCCAGACGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATGTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCt                                 <  2:3773919/92‑1 (MQ=255)
                                                    ggCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTg                                <  2:1167374/93‑1 (MQ=255)
                                                     gCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTgg                               <  1:1548244/93‑1 (MQ=255)
                                                       cAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGtt                             >  2:1785906/1‑93 (MQ=255)
                                                       cAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGtt                             <  2:2872031/93‑1 (MQ=255)
                                                        aGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTg                            >  2:4195652/1‑93 (MQ=255)
                                                         ggCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGa                           >  2:4123519/1‑93 (MQ=255)
                                                          gCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAg                          >  2:3128363/1‑93 (MQ=255)
                                                            gtagtaAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCa                        <  2:5862252/93‑1 (MQ=255)
                                                            gtagtaAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCa                        >  1:4178384/1‑93 (MQ=255)
                                                              agtaAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGt                      <  1:5325957/93‑1 (MQ=255)
                                                              agtaAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGt                      >  2:2515244/1‑93 (MQ=255)
                                                                taAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTAg                    <  1:2052230/93‑1 (MQ=255)
                                                                 aaaCGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTAGc                   >  1:3704104/1‑93 (MQ=255)
                                                                 aaaCGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTAGc                   <  1:2519252/93‑1 (MQ=255)
                                                                 aaaCGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTAGc                   >  1:4887434/1‑93 (MQ=255)
                                                                  aaCGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTAGCg                  <  2:2811912/93‑1 (MQ=255)
                                                                   aCGGCAGATTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTAGCGt                 <  2:5956778/93‑1 (MQ=255)
                                                                   aCGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTAGCGt                 >  2:690587/1‑93 (MQ=255)
                                                                   aCGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTAGCGt                 <  2:5123478/93‑1 (MQ=255)
                                                                   aCGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTAGCGt                 <  2:3701613/93‑1 (MQ=255)
                                                                     ggCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTAGCGTCg               >  1:4740587/1‑93 (MQ=255)
                                                                     ggCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTAGCGTCg               >  1:4228562/1‑93 (MQ=255)
                                                                      gCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTAGCGTCGc              <  1:2642784/93‑1 (MQ=255)
                                                                        agagTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTAGCGTCGCCa            <  1:1648903/93‑1 (MQ=255)
                                                                        agagTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTAGCGTCGCCa            >  1:5654474/1‑93 (MQ=255)
                                                                         gagTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTAGCGTCGCCAg           >  1:3155772/1‑93 (MQ=255)
                                                                           gttgttCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTAGCGTCGCCAGCa         >  1:454179/1‑93 (MQ=255)
                                                                            ttgttCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTAGCGTCGCCAGCaa        <  2:540848/93‑1 (MQ=255)
                                                                            ttgttCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTAGCGTCGCCAGCaa        <  2:4915409/93‑1 (MQ=255)
                                                                              gttCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTAGCGTCGCCAGCAAtt      <  1:390689/93‑1 (MQ=255)
                                                                              gttCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTAGCGTCGCCAGCAAtt      <  1:6015927/93‑1 (MQ=255)
                                                                               ttCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTAGCGTCGCCAGCAAttt     >  2:1579873/1‑93 (MQ=255)
                                                                                 cTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTAGCGTCGCCAGCAAttttt   >  1:4318926/1‑93 (MQ=255)
                                                                                 cTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTAGCGTCGCCAGCAAttttt   <  1:2958287/93‑1 (MQ=255)
                                                                                  tGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTAGCGTCGCCAGCAAtttttt  <  2:4495947/93‑1 (MQ=255)
                                                                                       ||                                                                                      
GTAATGTCATCTCTGGCACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGAGCCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTAGCGTCGCCAGCAATTTTTT  >  NC_000913/3960491‑3960665

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TAGCCTGTAATGTCATCTCTGGCACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGAGCCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTAGCGTCGCCAGCAATTTTTTCT  >  NC_000913/3960485‑3960667
                                                                                             |                                                                                         
TAGCCTGTAATGTCATCTCTGGCACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGAC                                                                                           <  SRR4302533.309144/94‑1 (MQ=60)
TAGCCTGTAATGTCATCTCTGGCACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGAC                                                                                           >  SRR4302533.4621051/1‑94 (MQ=60)
TAGCCTGTAATGTCATCTCTGGCACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGAC                                                                                           <  SRR4302533.2239891/94‑1 (MQ=60)
   CCTGTAATGTCATCTCTGGCACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGA‑‑CGCG                                                                                      >  SRR4302533.732550/1‑94 (MQ=60)
   CCTGTAATGTCATCTCTGGCACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGA‑‑CGCG                                                                                      >  SRR4302533.4071503/1‑94 (MQ=60)
     TGTAATTTCATCTCTGGCACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCG                                                                                      >  SRR4302533.4280757/1‑94 (MQ=60)
     TGTAATGTCATCTCTGGCACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCG                                                                                      <  SRR4302533.5987673/94‑1 (MQ=60)
     TGTAATGTCATCTCTGGCACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCG                                                                                      <  SRR4302533.2388210/94‑1 (MQ=60)
     TGTAATGTCATCTCTGGCACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCG                                                                                      <  SRR4302533.704772/94‑1 (MQ=60)
       TAATGTCATCTCTGGCACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGC                                                                                    >  SRR4302533.4744646/1‑94 (MQ=60)
       TAATGTCATCTCTGGCACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGC                                                                                    <  SRR4302533.3682952/94‑1 (MQ=60)
        AATGTCATCTCTGGCACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCG                                                                                   >  SRR4302533.2011581/1‑94 (MQ=60)
        AATGTCATCTCTGGCACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCG                                                                                   <  SRR4302533.3083723/94‑1 (MQ=60)
         ATGTCATCTCTGGCACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGG                                                                                  >  SRR4302533.6306635/1‑94 (MQ=60)
         ATGTCATCTCTGGCACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGG                                                                                  >  SRR4302533.1734812/1‑94 (MQ=60)
         ATGTCATCTCTGGCACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGG                                                                                  >  SRR4302533.6141996/1‑94 (MQ=60)
         ATGTCATCTCTGGCACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGG                                                                                  >  SRR4302533.2776173/1‑94 (MQ=60)
          TGTCATCTCTGGCACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGT                                                                                 >  SRR4302533.2605170/1‑94 (MQ=60)
           GTCATCTCTGGCACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTA                                                                                >  SRR4302533.739148/1‑94 (MQ=60)
            TCATCTCTGGCACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTAC                                                                               <  SRR4302533.750883/94‑1 (MQ=60)
             CATCTCTGGCACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACG                                                                              >  SRR4302533.5680581/1‑94 (MQ=60)
             CATCTCTGGCACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACG                                                                              <  SRR4302533.5753920/94‑1 (MQ=60)
              ATCTCTGGCACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGG                                                                             >  SRR4302533.3149220/1‑94 (MQ=60)
                CTCTGGCACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCA                                                                           <  SRR4302533.3298835/94‑1 (MQ=60)
                CTCTGGCACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCA                                                                           >  SRR4302533.1726674/1‑94 (MQ=60)
                 TCTGGCACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCAT                                                                          <  SRR4302533.371576/94‑1 (MQ=60)
                 TCTGGCACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCAT                                                                          >  SRR4302533.2684702/1‑94 (MQ=60)
                 TCTGGCACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCAT                                                                          >  SRR4302533.5016754/1‑94 (MQ=60)
                  CTGGCACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATT                                                                         <  SRR4302533.4320879/94‑1 (MQ=60)
                  CTGGCACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATT                                                                         >  SRR4302533.2650809/1‑94 (MQ=60)
                   TGGCACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTT                                                                        >  SRR4302533.5787474/1‑94 (MQ=60)
                    GGCACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTT                                                                       <  SRR4302533.2383680/94‑1 (MQ=60)
                     GCACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTG                                                                      <  SRR4302533.4231292/94‑1 (MQ=60)
                     GCACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTG                                                                      >  SRR4302533.836147/1‑94 (MQ=60)
                      CACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGC                                                                     >  SRR4302533.946550/1‑94 (MQ=60)
                      CACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGC                                                                     <  SRR4302533.2606429/94‑1 (MQ=60)
                       ACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCT                                                                    >  SRR4302533.626033/1‑94 (MQ=60)
                       ACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCT                                                                    >  SRR4302533.3056332/1‑94 (MQ=60)
                       ACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCT                                                                    <  SRR4302533.4760759/94‑1 (MQ=60)
                        CGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTG                                                                   >  SRR4302533.5052110/1‑94 (MQ=60)
                        CGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTG                                                                   <  SRR4302533.1423397/94‑1 (MQ=60)
                         GAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGA                                                                  >  SRR4302533.3257274/1‑94 (MQ=60)
                          AGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGAT                                                                 >  SRR4302533.5542670/1‑94 (MQ=60)
                          AGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGAT                                                                 <  SRR4302533.5812016/94‑1 (MQ=60)
                          AGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGAT                                                                 <  SRR4302533.2195406/94‑1 (MQ=60)
                            ATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGC                                                               >  SRR4302533.92576/1‑94 (MQ=60)
                             TCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCA                                                              <  SRR4302533.1179320/94‑1 (MQ=60)
                             TCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCA                                                              <  SRR4302533.673265/94‑1 (MQ=60)
                              CGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAG                                                             <  SRR4302533.2751991/94‑1 (MQ=60)
                              CGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAG                                                             >  SRR4302533.5344477/1‑94 (MQ=60)
                              CGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAG                                                             >  SRR4302533.3208720/1‑94 (MQ=60)
                              CGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAG                                                             <  SRR4302533.1130596/94‑1 (MQ=60)
                               GTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGC                                                            >  SRR4302533.2341852/1‑94 (MQ=60)
                                TCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCG                                                           <  SRR4302533.3741722/94‑1 (MQ=60)
                                TCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCG                                                           <  SRR4302533.2576766/94‑1 (MQ=60)
                                TCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCG                                                           <  SRR4302533.4499916/94‑1 (MQ=60)
                                TCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCG                                                           <  SRR4302533.213518/94‑1 (MQ=60)
                                 CACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGA                                                          >  SRR4302533.924168/1‑94 (MQ=60)
                                 CACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGA                                                          <  SRR4302533.6105826/94‑1 (MQ=60)
                                 CACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGA                                                          <  SRR4302533.1512489/94‑1 (MQ=60)
                                 CACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGA                                                          <  SRR4302533.707392/94‑1 (MQ=60)
                                  ACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGAT                                                         <  SRR4302533.5990013/94‑1 (MQ=60)
                                   CGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGTTGATGCAGCGATG                                                        <  SRR4302533.3428669/94‑1 (MQ=60)
                                   CGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATG                                                        <  SRR4302533.2697940/94‑1 (MQ=60)
                                   CGGCGACGGCGGACAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATG                                                        <  SRR4302533.3967969/94‑1 (MQ=60)
                                     GCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAG                                                      <  SRR4302533.5253231/94‑1 (MQ=60)
                                     GCGACGGCTGACAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAG                                                      <  SRR4302533.3815067/94‑1 (MQ=60)
                                      CGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGA                                                     <  SRR4302533.5609920/94‑1 (MQ=60)
                                      CGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGA                                                     <  SRR4302533.4235783/94‑1 (MQ=60)
                                       GACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGGG                                                    >  SRR4302533.5292178/1‑94 (MQ=60)
                                       GACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAG                                                    <  SRR4302533.2033991/94‑1 (MQ=60)
                                       GACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAG                                                    <  SRR4302533.2494316/94‑1 (MQ=60)
                                       GACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAG                                                    <  SRR4302533.5993874/94‑1 (MQ=60)
                                       GACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAG                                                    >  SRR4302533.950272/1‑94 (MQ=60)
                                        ACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGA                                                   >  SRR4302533.5920246/1‑94 (MQ=60)
                                         CGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGAT                                                  <  SRR4302533.4273034/94‑1 (MQ=60)
                                          GGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATC                                                 <  SRR4302533.3680596/94‑1 (MQ=60)
                                           GCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCG                                                <  SRR4302533.1021060/94‑1 (MQ=60)
                                           GCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCG                                                <  SRR4302533.1950168/94‑1 (MQ=60)
                                            CTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGA                                               <  SRR4302533.5308441/94‑1 (MQ=60)
                                            CTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGA                                               <  SRR4302533.3682463/94‑1 (MQ=60)
                                             TGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGAC                                              <  SRR4302533.978059/94‑1 (MQ=60)
                                              GGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACC                                             <  SRR4302533.1583145/94‑1 (MQ=60)
                                              GGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACC                                             <  SRR4302533.6128786/94‑1 (MQ=60)
                                              GGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACC                                             <  SRR4302533.1250073/94‑1 (MQ=60)
                                              GGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACC                                             <  SRR4302533.451313/94‑1 (MQ=60)
                                              GGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACC                                             <  SRR4302533.1837316/94‑1 (MQ=60)
                                               GCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCG                                            >  SRR4302533.837744/1‑94 (MQ=60)
                                               GCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCG                                            <  SRR4302533.1503577/94‑1 (MQ=60)
                                               GCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCG                                            <  SRR4302533.2370424/94‑1 (MQ=60)
                                               GCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCG                                            >  SRR4302533.5156184/1‑94 (MQ=60)
                                                CAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGG                                           <  SRR4302533.5409347/94‑1 (MQ=60)
                                                 AAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGA                                          >  SRR4302533.483739/1‑94 (MQ=60)
                                                  AAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGAT                                         <  SRR4302533.3002712/94‑1 (MQ=60)
                                                  AAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGAT                                         >  SRR4302533.4885520/1‑94 (MQ=60)
                                                   AAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATT                                        >  SRR4302533.857282/1‑94 (MQ=60)
                                                   AAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATT                                        <  SRR4302533.1715496/94‑1 (MQ=60)
                                                       GATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCCGCGATGAGAGATCGACCGGATTAGTC                                    >  SRR4302533.1155318/1‑94 (MQ=60)
                                                        ATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCT                                   <  SRR4302533.243909/94‑1 (MQ=60)
                                                         TGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTG                                  >  SRR4302533.885823/1‑94 (MQ=60)
                                                          GGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGG                                 <  SRR4302533.5563495/94‑1 (MQ=60)
                                                          GGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGG                                 <  SRR4302533.3753982/94‑1 (MQ=60)
                                                           GCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGT                                <  SRR4302533.1557574/94‑1 (MQ=60)
                                                            CCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTT                               <  SRR4302533.3184938/94‑1 (MQ=60)
                                                             CAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTG                              >  SRR4302533.3885520/1‑94 (MQ=60)
                                                              AGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGA                             <  SRR4302533.5250948/94‑1 (MQ=60)
                                                              AGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGA                             <  SRR4302533.2020200/94‑1 (MQ=60)
                                                               GGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAG                            <  SRR4302533.5003591/94‑1 (MQ=60)
                                                                 CGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCA                          >  SRR4302533.4203698/1‑94 (MQ=60)
                                                                 CGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCA                          >  SRR4302533.3264376/1‑94 (MQ=60)
                                                                  GTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAG                         >  SRR4302533.3944124/1‑94 (MQ=60)
                                                                  GTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAG                         <  SRR4302533.3829183/94‑1 (MQ=60)
                                                                  GTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAG                         <  SRR4302533.139585/94‑1 (MQ=60)
                                                                    AGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTA                       <  SRR4302533.3571051/94‑1 (MQ=60)
                                                                    AGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTA                       <  SRR4302533.5357877/94‑1 (MQ=60)
                                                                     GTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTAG                      <  SRR4302533.724358/94‑1 (MQ=60)
                                                                     GTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATACAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTAG                      <  SRR4302533.3036303/94‑1 (MQ=60)
                                                                      TAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTAGC                     <  SRR4302533.2064894/94‑1 (MQ=60)
                                                                      TAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTAGC                     <  SRR4302533.5156136/94‑1 (MQ=60)
                                                                      TAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTAGC                     >  SRR4302533.4917047/1‑94 (MQ=60)
                                                                      TAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTAGC                     <  SRR4302533.2718759/94‑1 (MQ=60)
                                                                      TAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTAGC                     >  SRR4302533.3726855/1‑94 (MQ=60)
                                                                       AAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTAGCG                    >  SRR4302533.1542914/1‑94 (MQ=60)
                                                                       AAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTAGCG                    <  SRR4302533.2122847/94‑1 (MQ=60)
                                                                       AAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTAGCG                    <  SRR4302533.2534774/94‑1 (MQ=60)
                                                                        AACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTAGCGT                   <  SRR4302533.432899/94‑1 (MQ=60)
                                                                          CGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTAGCGTCG                 >  SRR4302533.4254128/1‑94 (MQ=60)
                                                                          CGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTAGCGTCG                 >  SRR4302533.4769331/1‑94 (MQ=60)
                                                                          CGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTAGCGTCG                 >  SRR4302533.5419012/1‑94 (MQ=60)
                                                                            GCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTAGCGTCGCC               <  SRR4302533.5900271/94‑1 (MQ=60)
                                                                            GCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTAGCGTCGCC               <  SRR4302533.2659022/94‑1 (MQ=60)
                                                                             CAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTAGCGTCGCCA              <  SRR4302533.2127681/94‑1 (MQ=60)
                                                                             CAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTAGCGTCGCCA              >  SRR4302533.5688648/1‑94 (MQ=60)
                                                                              AGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTAGCGTCGCCAG             >  SRR4302533.3175174/1‑94 (MQ=60)
                                                                              AGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTAGCGTCGCCAG             >  SRR4302533.555703/1‑94 (MQ=60)
                                                                              AGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTAGCGTCGCCAG             >  SRR4302533.2868031/1‑94 (MQ=60)
                                                                              AGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTAGCGTCGCCAG             <  SRR4302533.2194657/94‑1 (MQ=60)
                                                                              AGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTAGCGTCGCCAG             <  SRR4302533.1658895/94‑1 (MQ=60)
                                                                              AGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTAGCGTCGACAG             >  SRR4302533.2868032/1‑94 (MQ=60)
                                                                                AGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTAGCGTCGCCAGCA           >  SRR4302533.1118830/1‑94 (MQ=60)
                                                                                AGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTAGCGTCGCCAGCA           >  SRR4302533.6075978/1‑94 (MQ=60)
                                                                                AGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTAGCGTCGCCAGCA           >  SRR4302533.456894/1‑94 (MQ=60)
                                                                                  TTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTAGCGTCGCCAGCAAT         >  SRR4302533.5862019/1‑94 (MQ=60)
                                                                                   TGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTAGCGTCGCCAGCAATT        >  SRR4302533.2547927/1‑94 (MQ=60)
                                                                                    GTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTAGCGTCGCCAGCAATTT       <  SRR4302533.6052381/94‑1 (MQ=60)
                                                                                    GTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTAGCGTCGCCAGCAATTT       <  SRR4302533.393052/94‑1 (MQ=60)
                                                                                      TCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTAGCGTCGCCAGCAATTTTT     >  SRR4302533.5543915/1‑94 (MQ=60)
                                                                                      TCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTAGCGTCGCCAGCAATTTTT     >  SRR4302533.4344939/1‑94 (MQ=60)
                                                                                       CTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTAGCGTCGCCAGCAATTTTTT    <  SRR4302533.2976370/94‑1 (MQ=60)
                                                                                        TGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTAGCGTCGCCAGCAATTTTTTC   >  SRR4302533.5480140/1‑94 (MQ=60)
                                                                                        TGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTAGCGTCGCCAGCAATTTTTTC   >  SRR4302533.2936874/1‑94 (MQ=60)
                                                                                         GCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTAGCGTCGCCAGCAATTTTTTCT  >  SRR4302533.343280/1‑94 (MQ=60)
                                                                                             |                                                                                         
TAGCCTGTAATGTCATCTCTGGCACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGAGCCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTAGCGTCGCCAGCAATTTTTTCT  >  NC_000913/3960485‑3960667

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑