Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,004,979 +G coding (1511/1833 nt) recQ → ATP‑dependent DNA helicase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,004,9791.G100.0% 618.6 / NA 191L504R (CTC→CGC) recQATP‑dependent DNA helicase
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (94/97);  total (94/97)

Only 100 of 209 total aligned reads displayed.

CGTGATCCGCCAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCT‑CGCG‑‑GAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAA  >  NC_000913/4004887‑4005060
                                                                                             |                                                                                   
 gTGATCCGCCAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCtg                                                                                     >  1:1019993/1‑92 (MQ=255)
  tGATCCGCCAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCtgc                                                                                    >  2:2755093/1‑91 (MQ=255)
  tGATCCGCCAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCtgc                                                                                    <  1:3728259/93‑3 (MQ=255)
   gATCCGCCAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCtgcg                                                                                   >  2:5450747/1‑90 (MQ=255)
    aTCCGCCAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCtgcgc                                                                                  <  1:4136890/93‑5 (MQ=255)
         ccAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCg‑‑gcga                                                                           >  1:177210/1‑90 (MQ=255)
          cAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCTCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCg‑‑gcgaa                                                                          <  1:3379572/93‑5 (MQ=255)
                aTTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCctct                                                                      >  2:2324309/1‑93 (MQ=255)
                 ttCGCCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCtt                                                                     <  1:3141190/93‑1 (MQ=255)
                 ttCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCtt                                                                     <  2:472872/93‑1 (MQ=255)
                 ttCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCtt                                                                     <  1:1421610/93‑1 (MQ=255)
                    aCCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGc                                                                  >  2:3772512/1‑93 (MQ=255)
                     ccTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCa                                                                 >  1:476749/1‑93 (MQ=255)
                      cTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCaa                                                                >  1:5923282/1‑93 (MQ=255)
                      cTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCaa                                                                <  2:6041194/93‑1 (MQ=255)
                       tCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAAc                                                               >  1:5611747/1‑93 (MQ=255)
                       tCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAAc                                                               >  1:329365/1‑93 (MQ=255)
                        cGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACt                                                              >  1:5416813/1‑93 (MQ=255)
                        cGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACt                                                              >  1:2665722/1‑93 (MQ=255)
                         ggCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACtt                                                             <  1:4201894/93‑1 (MQ=255)
                         ggCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACtt                                                             <  2:2665722/93‑1 (MQ=255)
                          gCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTtg                                                            <  2:4136421/93‑1 (MQ=255)
                          gCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTtg                                                            <  2:1787014/93‑1 (MQ=255)
                           ccTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTtgc                                                           <  2:116558/93‑1 (MQ=255)
                            cTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTtgcc                                                          >  1:3244999/1‑93 (MQ=255)
                            cTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTtgcc                                                          >  1:375247/1‑93 (MQ=255)
                             tGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTtgccg                                                         >  2:2603433/1‑93 (MQ=255)
                             tGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTtgccg                                                         >  2:1409522/1‑93 (MQ=255)
                             tGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTtgccg                                                         >  2:5838077/1‑93 (MQ=255)
                                 gACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTtgccgtgcc                                                     >  1:5346037/1‑93 (MQ=255)
                                 gACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTtgccgtgcc                                                     >  2:5746956/1‑93 (MQ=255)
                                 gACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTtgccgtgcc                                                     >  1:2225522/1‑93 (MQ=255)
                                  aCGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCTACTtgccgtgccg                                                    >  2:5490172/1‑93 (MQ=255)
                                  aCGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTtgccgtgccg                                                    >  1:2137368/1‑93 (MQ=255)
                                   cGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAAACCTCTTTGCAACTTGCCGTGCcgc                                                   <  1:3035147/93‑1 (MQ=255)
                                    gCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCcgcg                                                  <  1:2721216/93‑1 (MQ=255)
                                     cAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGt                                                 >  2:1973360/1‑93 (MQ=255)
                                      aaaaTATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTa                                                <  2:5556947/93‑1 (MQ=255)
                                      aaaaTATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTa                                                >  2:1887824/1‑93 (MQ=255)
                                        aaTATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATc                                              <  1:2871766/93‑1 (MQ=255)
                                        aaTATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGAGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATc                                              >  1:1171060/1‑93 (MQ=255)
                                           atTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTg                                           >  1:5875216/1‑93 (MQ=255)
                                            ttGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTgg                                          >  1:4895783/1‑93 (MQ=255)
                                             tGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGgc                                         <  1:6248955/93‑1 (MQ=255)
                                              gCCCAGCATTCTGCCCTACAACTGACGGAGTCCGCGCGGCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGgcg                                        <  1:1528993/93‑1 (MQ=255)
                                                  aGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCa                                    >  1:400987/1‑93 (MQ=255)
                                                    cATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCaaa                                  >  2:2448760/1‑93 (MQ=255)
                                                    cATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCaaa                                  <  1:1572688/93‑1 (MQ=255)
                                                     aTTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAAc                                 >  2:3171050/1‑93 (MQ=255)
                                                     aTTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAAc                                 >  1:2159908/1‑93 (MQ=255)
                                                      ttCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAAcc                                >  1:57628/1‑93 (MQ=255)
                                                      ttCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAAcc                                <  2:1258829/93‑1 (MQ=255)
                                                      ttCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAAcc                                <  1:5267505/93‑1 (MQ=255)
                                                        cTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGa                              <  1:1648239/93‑1 (MQ=255)
                                                        cTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGa                              <  2:121320/93‑1 (MQ=255)
                                                         tGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGaa                             >  2:355688/1‑93 (MQ=255)
                                                         tGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGaa                             <  1:826125/93‑1 (MQ=255)
                                                          gCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGaaa                            >  1:3651219/1‑93 (MQ=255)
                                                          gCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGaaa                            <  2:2910997/93‑1 (MQ=255)
                                                          gCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGaaa                            <  2:2654682/93‑1 (MQ=255)
                                                          gCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGaaa                            >  2:5956177/1‑93 (MQ=255)
                                                            ccTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGc                          <  1:120518/93‑1 (MQ=255)
                                                            ccTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGc                          <  2:5673688/93‑1 (MQ=255)
                                                             cTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCg                         >  1:3951979/1‑93 (MQ=255)
                                                              tACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGa                        >  1:2572263/1‑93 (MQ=255)
                                                               aCAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGAt                       <  2:3654673/93‑1 (MQ=255)
                                                               aCAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGAt                       <  1:3842594/93‑1 (MQ=255)
                                                                 aaCTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGc                     <  1:56444/93‑1 (MQ=255)
                                                                 aaCTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGc                     >  1:5098607/1‑93 (MQ=255)
                                                                  aCTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCa                    >  2:3268734/1‑93 (MQ=255)
                                                                  aCTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCa                    >  2:960315/1‑93 (MQ=255)
                                                                  aCTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCa                    <  2:1058371/93‑1 (MQ=255)
                                                                  aCTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGAAACTTGCCGTGCCGCGTATCGTGGCGCTCCAAACGTAAGCGATGCa                    >  2:4452810/1‑93 (MQ=255)
                                                                   cTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGGTGCAg                   >  1:1397753/1‑93 (MQ=255)
                                                                   cTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAg                   <  2:4172560/93‑1 (MQ=255)
                                                                    tGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGa                  <  1:1951798/93‑1 (MQ=255)
                                                                     gACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGaa                 >  1:4358371/1‑93 (MQ=255)
                                                                     gACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGaa                 >  1:5173511/1‑93 (MQ=255)
                                                                      aCAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGaaa                <  1:1109496/93‑1 (MQ=255)
                                                                      aCAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGaaa                <  1:6151220/93‑1 (MQ=255)
                                                                      aCAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGaaa                <  2:3750858/93‑1 (MQ=255)
                                                                      aCAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGaaa                <  2:519018/93‑1 (MQ=255)
                                                                       cAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAAt               <  1:5788524/93‑1 (MQ=255)
                                                                       cAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAAt               <  1:5042929/93‑1 (MQ=255)
                                                                        agagGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATc              <  2:4474593/93‑1 (MQ=255)
                                                                        agagGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATc              >  1:3577129/1‑93 (MQ=255)
                                                                        agagGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATc              <  2:2702180/93‑1 (MQ=255)
                                                                         gagGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCg             >  1:3124294/1‑93 (MQ=255)
                                                                           ggCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGtt           <  1:899722/93‑1 (MQ=255)
                                                                             ccGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTcg         <  1:5983589/93‑1 (MQ=255)
                                                                               gcgcgcCGGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTcggc       <  2:5776/93‑1 (MQ=255)
                                                                               gcgcgcCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTcggc       >  2:4019/1‑93 (MQ=255)
                                                                               gcgcgcCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTcggc       >  2:3424466/1‑93 (MQ=255)
                                                                               gcgcgcCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTaggc       >  2:754204/1‑93 (MQ=255)
                                                                                cgcgcCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTcggcg      <  1:507866/93‑1 (MQ=255)
                                                                                cgcgcCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTcggcg      >  1:668009/1‑93 (MQ=255)
                                                                                  cgcCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCggcggc    <  1:4622974/93‑1 (MQ=255)
                                                                                  cgcCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCggcggc    >  1:3500387/1‑93 (MQ=255)
                                                                                   gcCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCa   >  2:3042247/1‑93 (MQ=255)
                                                                                    cccGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCaa  <  1:5838077/93‑1 (MQ=255)
                                                                                             |                                                                                   
CGTGATCCGCCAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCT‑CGCG‑‑GAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAA  >  NC_000913/4004887‑4005060

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GCGTGATCCGCCAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCT‑CGCG‑‑GAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAACTA  >  NC_000913/4004886‑4005063
                                                                                             |                                                                                       
GCGTGATCCGCCAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCT                                                                                         <  SRR4302533.5502496/94‑1 (MQ=60)
 CGTGATCCGCCAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCT‑G                                                                                       >  SRR4302533.1025987/1‑94 (MQ=60)
 CGTGATCCGCCAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCT‑G                                                                                       <  SRR4302533.3726641/94‑1 (MQ=60)
 CGTGATCCGCCAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCACCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCT‑G                                                                                       >  SRR4302533.1828699/1‑94 (MQ=60)
  GTGATCCGCCAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTgc                                                                                       >  SRR4302533.5035507/1‑92 (MQ=60)
   TGATCCGCCAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCT‑‑GCG                                                                                    <  SRR4302533.3751129/94‑1 (MQ=60)
   TGATCCGCCAGCTGATTCACCTCGGCCTGGCGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCT‑‑GCG                                                                                    >  SRR4302533.4917720/1‑94 (MQ=60)
    GATCCGCCAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGC                                                                                     >  SRR4302533.4130101/1‑94 (MQ=60)
     ATCCGCCAGCTGATTCTCCTCGCCCTGGTGACGCAAAATATTGCCAAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCG                                                                                    <  SRR4302533.3795946/94‑1 (MQ=60)
     ATCCGCCAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCG                                                                                    >  SRR4302533.2776761/1‑94 (MQ=60)
     ATCCGCCAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCG                                                                                    <  SRR4302533.4273645/94‑1 (MQ=60)
     ATCCGCCAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCG                                                                                    <  SRR4302533.4161994/94‑1 (MQ=60)
     ATCCGCCAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCG                                                                                    <  SRR4302533.386978/94‑1 (MQ=60)
      TCCGCCAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCG‑‑G                                                                                 <  SRR4302533.3210563/94‑1 (MQ=60)
       CCGCCAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTgcgcggc                                                                                  <  SRR4302533.2562717/94‑8 (MQ=60)
       CCGCCAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTgcgcggc                                                                                  >  SRR4302533.2166299/1‑87 (MQ=60)
       CCGCCAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTgcgcggc                                                                                  >  SRR4302533.4246474/1‑87 (MQ=60)
         GCCAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTgcgcggcga                                                                                >  SRR4302533.178320/1‑85 (MQ=60)
          CCAGCTGATTCACCTCGGCCTTGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGTCGCGCGCCCGGTGCTgcgcagcgaa                                                                               >  SRR4302533.579756/1‑84 (MQ=60)
           CAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCTCTACAACTGACAGAGGCCGCGCGCCCGGTGCTgcgcggcgaat                                                                              <  SRR4302533.3400434/94‑12 (MQ=60)
           CAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTgcgcggcgaat                                                                              >  SRR4302533.4314004/1‑83 (MQ=60)
            AGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTgcgcggcgaatc                                                                             >  SRR4302533.1930749/1‑82 (MQ=60)
            AGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTgcgcggcgaatc                                                                             >  SRR4302533.754483/1‑82 (MQ=60)
             GCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTgcgcggcgaatcc                                                                            >  SRR4302533.5188368/1‑81 (MQ=60)
              CTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCT                                                                           >  SRR4302533.5054336/1‑94 (MQ=60)
              CTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCT                                                                           >  SRR4302533.5443741/1‑94 (MQ=60)
              CTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCT                                                                           >  SRR4302533.4009828/1‑94 (MQ=60)
               TGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTC                                                                          >  SRR4302533.1667563/1‑94 (MQ=60)
               TGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGGGCTGCGCGGCGAATCCTC                                                                          >  SRR4302533.977645/1‑94 (MQ=60)
                GATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCT                                                                         >  SRR4302533.365412/1‑94 (MQ=60)
                GATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCT                                                                         <  SRR4302533.4721311/94‑1 (MQ=60)
                GATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCT                                                                         <  SRR4302533.5855042/94‑1 (MQ=60)
                  TTCGCCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTT                                                                       <  SRR4302533.3160501/94‑1 (MQ=60)
                  TTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTT                                                                       <  SRR4302533.1430208/94‑1 (MQ=60)
                    CACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGC                                                                     >  SRR4302533.5805117/1‑94 (MQ=60)
                    CACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGC                                                                     >  SRR4302533.847798/1‑94 (MQ=60)
                     ACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCA                                                                    >  SRR4302533.479595/1‑94 (MQ=60)
                     ACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCA                                                                    >  SRR4302533.3096333/1‑94 (MQ=60)
                      CCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAA                                                                   >  SRR4302533.5959084/1‑94 (MQ=60)
                       CTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAAC                                                                  >  SRR4302533.5645665/1‑94 (MQ=60)
                       CTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAAC                                                                  >  SRR4302533.331374/1‑94 (MQ=60)
                       CTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAAC                                                                  <  SRR4302533.1568097/94‑1 (MQ=60)
                        TCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACT                                                                 >  SRR4302533.5449396/1‑94 (MQ=60)
                        TCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACT                                                                 >  SRR4302533.2682079/1‑94 (MQ=60)
                        TCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACT                                                                 >  SRR4302533.2661043/1‑94 (MQ=60)
                          GGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTG                                                               >  SRR4302533.804955/1‑94 (MQ=60)
                          GGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTG                                                               <  SRR4302533.4227316/94‑1 (MQ=60)
                           GCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGC                                                              >  SRR4302533.3147688/1‑94 (MQ=60)
                            CCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCC                                                             >  SRR4302533.377511/1‑94 (MQ=60)
                            CCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCC                                                             <  SRR4302533.1413018/94‑1 (MQ=60)
                            CCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCC                                                             >  SRR4302533.3265002/1‑94 (MQ=60)
                            CCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCC                                                             >  SRR4302533.420054/1‑94 (MQ=60)
                            CCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCC                                                             >  SRR4302533.5842481/1‑94 (MQ=60)
                               GGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTG                                                          <  SRR4302533.3435479/94‑1 (MQ=60)
                                GTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGC                                                         <  SRR4302533.974531/94‑1 (MQ=60)
                                 TGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCC                                                        >  SRR4302533.5658953/1‑94 (MQ=60)
                                 TGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCC                                                        >  SRR4302533.5378100/1‑94 (MQ=60)
                                 TGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCC                                                        <  SRR4302533.4477039/94‑1 (MQ=60)
                                 TGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCC                                                        >  SRR4302533.2239261/1‑94 (MQ=60)
                                  GACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCG                                                       >  SRR4302533.2150548/1‑94 (MQ=60)
                                    CGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCG                                                     <  SRR4302533.4023479/94‑1 (MQ=60)
                                    CGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAAACCTCTTTGCAACTTGCCGTGCCGCG                                                     <  SRR4302533.3053720/94‑1 (MQ=60)
                                     GCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGT                                                    <  SRR4302533.2737918/94‑1 (MQ=60)
                                     CCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGT                                                    <  SRR4302533.1754594/94‑1 (MQ=60)
                                       AAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTAT                                                  >  SRR4302533.3367837/1‑94 (MQ=60)
                                        AAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATC                                                 <  SRR4302533.3562624/94‑1 (MQ=60)
                                        AAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGAGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATC                                                 >  SRR4302533.1178090/1‑94 (MQ=60)
                                         AATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCG                                                <  SRR4302533.2889437/94‑1 (MQ=60)
                                         AATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCG                                                <  SRR4302533.6267665/94‑1 (MQ=60)
                                         AATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCG                                                <  SRR4302533.5104856/94‑1 (MQ=60)
                                          ATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAGTCCTCTTTGCAACTTGCCGTGCCGCGTATCGT                                               <  SRR4302533.1160810/94‑1 (MQ=60)
                                          ATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGT                                               <  SRR4302533.5604102/94‑1 (MQ=60)
                                           TATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTG                                              >  SRR4302533.5111728/1‑94 (MQ=60)
                                           TATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTG                                              >  SRR4302533.5910733/1‑94 (MQ=60)
                                            ATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGG                                             >  SRR4302533.4925449/1‑94 (MQ=60)
                                            ATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGG                                             <  SRR4302533.3238686/94‑1 (MQ=60)
                                              TGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCG                                           <  SRR4302533.6286343/94‑1 (MQ=60)
                                               GCCCAGCATTCTGCCCTACAACTGACGGAGTCCGCGCGGCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGC                                          <  SRR4302533.1538189/94‑1 (MQ=60)
                                                 CCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTC                                        >  SRR4302533.3032341/1‑94 (MQ=60)
                                                  CAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCA                                       >  SRR4302533.403406/1‑94 (MQ=60)
                                                  CAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCA                                       <  SRR4302533.3756064/94‑1 (MQ=60)
                                                   AGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAA                                      <  SRR4302533.1844340/94‑1 (MQ=60)
                                                     CATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAAC                                    >  SRR4302533.2173205/1‑94 (MQ=60)
                                                     CATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAAC                                    <  SRR4302533.1582159/94‑1 (MQ=60)
                                                     CATCCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAAC                                    <  SRR4302533.79101/94‑1 (MQ=60)
                                                      ATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACC                                   >  SRR4302533.5913821/1‑94 (MQ=60)
                                                      ATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACC                                   >  SRR4302533.58010/1‑94 (MQ=60)
                                                       TTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCG                                  <  SRR4302533.3360163/94‑1 (MQ=60)
                                                       TTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCG                                  <  SRR4302533.5299108/94‑1 (MQ=60)
                                                         CTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAA                                >  SRR4302533.1040962/1‑94 (MQ=60)
                                                         CTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAA                                <  SRR4302533.1658223/94‑1 (MQ=60)
                                                          TGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAA                               <  SRR4302533.830969/94‑1 (MQ=60)
                                                          TGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAA                               >  SRR4302533.3673667/1‑94 (MQ=60)
                                                           GCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCACACCGAAAG                              >  SRR4302533.543944/1‑94 (MQ=60)
                                                           GCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAG                              <  SRR4302533.2224953/94‑1 (MQ=60)
                                                           GCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAG                              <  SRR4302533.5371017/94‑1 (MQ=60)
                                                            CCCTACAACTGACCGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGC                             <  SRR4302533.2153653/94‑1 (MQ=60)
                                                             CCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCG                            <  SRR4302533.270883/94‑1 (MQ=60)
                                                             CCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCG                            >  SRR4302533.3976069/1‑94 (MQ=60)
                                                             CCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCG                            <  SRR4302533.121281/94‑1 (MQ=60)
                                                             CCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCG                            >  SRR4302533.62905/1‑94 (MQ=60)
                                                              CTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGA                           >  SRR4302533.5712185/1‑94 (MQ=60)
                                                              CTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGA                           >  SRR4302533.1185501/1‑94 (MQ=60)
                                                              CTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGA                           >  SRR4302533.2588089/1‑94 (MQ=60)
                                                               TACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGAT                          <  SRR4302533.6033779/94‑1 (MQ=60)
                                                                ACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATG                         <  SRR4302533.3866069/94‑1 (MQ=60)
                                                                 CAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGC                        >  SRR4302533.5129411/1‑94 (MQ=60)
                                                                  AACTGACAGAGGCCGCGCGCCCGTTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCA                       <  SRR4302533.4665829/94‑1 (MQ=60)
                                                                  AACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCA                       <  SRR4302533.56820/94‑1 (MQ=60)
                                                                   ACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGGTGCAG                      >  SRR4302533.1406178/1‑94 (MQ=60)
                                                                    CTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGA                     >  SRR4302533.3793075/1‑94 (MQ=60)
                                                                     TGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAA                    >  SRR4302533.5204766/1‑94 (MQ=60)
                                                                     TGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAA                    <  SRR4302533.1963694/94‑1 (MQ=60)
                                                                     TGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAA                    >  SRR4302533.4384678/1‑94 (MQ=60)
                                                                      GACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAA                   >  SRR4302533.5906508/1‑94 (MQ=60)
                                                                      GACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAA                   >  SRR4302533.2884522/1‑94 (MQ=60)
                                                                       ACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAAT                  <  SRR4302533.1116080/94‑1 (MQ=60)
                                                                       ACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAAT                  <  SRR4302533.6188241/94‑1 (MQ=60)
                                                                       ACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAAT                  <  SRR4302533.3297088/94‑1 (MQ=60)
                                                                        CAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATC                 <  SRR4302533.5823497/94‑1 (MQ=60)
                                                                        CAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATC                 <  SRR4302533.5073449/94‑1 (MQ=60)
                                                                        CAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATC                 >  SRR4302533.3599174/1‑94 (MQ=60)
                                                                         AGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAGATCG                >  SRR4302533.5467359/1‑94 (MQ=60)
                                                                         AGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCG                >  SRR4302533.3143499/1‑94 (MQ=60)
                                                                         AGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCG                >  SRR4302533.599892/1‑94 (MQ=60)
                                                                           AGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTT              >  SRR4302533.5170347/1‑94 (MQ=60)
                                                                           AGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTT              >  SRR4302533.1617606/1‑94 (MQ=60)
                                                                            GGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTC             <  SRR4302533.905002/94‑1 (MQ=60)
                                                                            GGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTC             >  SRR4302533.1471326/1‑94 (MQ=60)
                                                                              CCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGG           <  SRR4302533.6019825/94‑1 (MQ=60)
                                                                               CGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGC          <  SRR4302533.5268982/94‑1 (MQ=60)
                                                                                GCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCG         <  SRR4302533.1299733/94‑1 (MQ=60)
                                                                                GCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCG         >  SRR4302533.671956/1‑94 (MQ=60)
                                                                                 CGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGG        <  SRR4302533.510882/94‑1 (MQ=60)
                                                                                  GCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGC       >  SRR4302533.3521973/1‑94 (MQ=60)
                                                                                  GCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGC       <  SRR4302533.2127347/94‑1 (MQ=60)
                                                                                   CGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCA      <  SRR4302533.4650995/94‑1 (MQ=60)
                                                                                     CCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAAC    >  SRR4302533.1385068/1‑94 (MQ=60)
                                                                                     CCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAAC    <  SRR4302533.5873374/94‑1 (MQ=60)
                                                                                      CCGGTGCTGCGCGGCGAATCCTCTTTGCAATTCGCCTTGTCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAACT   <  SRR4302533.3901551/94‑1 (MQ=60)
                                                                                      CCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAACT   >  SRR4302533.4328543/1‑94 (MQ=60)
                                                                                      CCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAACT   <  SRR4302533.120965/94‑1 (MQ=60)
                                                                                      CCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAACT   <  SRR4302533.2342314/94‑1 (MQ=60)
                                                                                      CCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAACT   <  SRR4302533.309831/94‑1 (MQ=60)
                                                                                       CGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAACTA  >  SRR4302533.493595/1‑94 (MQ=60)
                                                                                             |                                                                                       
GCGTGATCCGCCAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCT‑CGCG‑‑GAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAACTA  >  NC_000913/4004886‑4005063

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑