Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,020,045 C→T A54V (GCG→GTG)  b3838 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,020,0450CT99.6% 968.9 / NA 270A54V (GCG→GTG) b3838hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base T (150/119);  total (151/119)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 277 total aligned reads displayed.

TGACCCAGGAGTTAAAACTCCAGGAGTTTCAGGACAGTCTGAAAAAGGTTGAAAAGGCGAGCCTCACTAACCTGACGCCCGAACTGAAAGCGTCGATGGATGAACTACGCCAGGCCGCGGAGTCGATGAAGCGTTCCTACGTTGCAAACGATCCTGAAAAGGCGAGCGATGAAGCGCACA  >  NC_000913/4019955‑4020134
                                                                                          |                                                                                         
tGACCCAGGAGTTAAAACTCCAGGAGTTTCAGGACAGTCTGAAAAAGGTTGAAAAGGCGAGCCTCACTAACCTGACGCCCGGACTGAAAgtgt                                                                                         >  2:879832/1‑93 (MQ=255)
  aCCCAGGAGTTAAAACTCCAGGAGTTTCAGGACAGTCTGAAAAAGGTTGAAAAGGCGAGCCTCACTAACCTGACGCCCGAACTGAAAGTGTCg                                                                                       >  2:2320899/1‑93 (MQ=255)
   cccAGGAGTTAAAACTCCAGGAGTTTCAGGACAGTCTGAAAAAGGTTGAAAAGGCGAGCCTCACTAACCTGACGCCCGAACTGAAAGTGTCga                                                                                      >  1:5528682/1‑93 (MQ=255)
   cccAGGAGTTAAAACTCCAGGAGTTTCAGGACAGTCTGAAAAAGGTTGAAAAGGCGAGCCTCACTAACCTGACGCCCGAACTGAAAGTGTCga                                                                                      <  2:1684500/93‑1 (MQ=255)
   cccAGGAGTTAAAACTCCAGGAGTTTCAGGACAGTCTGAAAAAGGTTGAAAAGGCGAGCCTCACTAACCTGACGCCCGAACTGAAAGTGTCga                                                                                      <  2:3377957/93‑1 (MQ=255)
    ccAGGAGTTAAAACTCCAGGAGTTTCAGGACAGTCTGAAAAAGGTTGAAAAGGCGAGCCTCACTAACCTGACGCCCGAACTGAAAGTGTCgat                                                                                     <  1:3410077/93‑1 (MQ=255)
    ccAGGAGTTAAAACTCCAGGAGTTTCAGGACAGTCTGAAAAAGGTTGAAAAGGCGAGCCTCACTAACCTGACGCCCGAACTGAAAGTGTCgat                                                                                     <  2:1949444/93‑1 (MQ=255)
     cAGGAGTTAAAACTCCAGGAGTTTCAGGACAGTCTGAAAAAGGTTGAAAAGGCGAGCCTCACTAACCTGACGCCCGAACTGAAAGTGTCgatg                                                                                    >  1:131964/1‑93 (MQ=255)
     cAGGAGTTAAAACTCCAGGAGTTTCAGGACAGTCTGAAAAAGGTTGAAAAGGCGAGCCTCACTAACCTGACGCCCGAACTGAAAGTGTCgatg                                                                                    <  1:5418799/93‑1 (MQ=255)
      aGGAGTTAAAACTCCAGGAGTTTCAGGACAGTCTGAAAAAGGTTGAAAAGGCGAGCCTCACTAACCTGACGCCCGAACTGAAAGTGTCgatgg                                                                                   >  1:1247700/1‑93 (MQ=255)
      aGGAGTTAAAACTCCAGGAGTTTCAGGACAGTCTGAAAAAGGTTGAAAAGGCGAGCCTCACTAACCTGACGCCCGAACTGAAAGTGTCgatgg                                                                                   >  2:1302067/1‑93 (MQ=255)
       ggAGTTAAAACTCCAGGAGTTTCAGGACAGTCTGAAAAAGGTTGAAAAGGCGAGCCTCACTAACCTGACGCCCGAACTGAAAGTGTCgatgga                                                                                  >  2:1776502/1‑93 (MQ=255)
          gTTAAAACTCCAGGAGTTTCAGGACAGTCTGAAAAAGGTTGAAAAGGCGAGCCTCACTAACCTGACGCCCGAACTGAAAGTGTCGATGGATGa                                                                               >  1:3918765/1‑93 (MQ=255)
            tAAAACTCCAGGAGTTTCAGGACAGTCTGAAAAAGGTTGAAAAGGCGAGCCTCACTAACCTGACGCCCGAACTGAAAGTGTCGATGGATGAAc                                                                             <  2:6080771/93‑1 (MQ=255)
              aaaCTCCAGGAGTTTCAGGACAGTCTGAAAAAGGTTGAAAAGGCGAGCCTCACTAACCTGACGCCCGAACTGAAAGTGTCGATGGATGAACTa                                                                           <  2:2233671/93‑1 (MQ=255)
                  tCCAGGAGTTTCAGGACAGTCTGAAAAAGGTTGAAAAGGCGAGCCTCACTACCCTAACGCCCGAACTGAAAGTGTCGTTGGATGAACTCCGca                                                                       >  2:3561754/1‑92 (MQ=255)
                   ccAGGAGTTTCAGGACAGTCTGAAAAAGGTTGAAAAGGCGAGCCTCACTAACCTGACGCCCGAACTGAAAGTGTCGATGGATGAACTATGCCa                                                                      <  2:3952906/93‑1 (MQ=255)
                   ccAGGAGTTTCAGGACAGTCTGAAAAAGGTTGAAAAGGCGAGCCTCACTAACCTGACGCCCGAACTGAAAGTGTCGATGGATGAACTACGCCa                                                                      <  1:2890597/93‑1 (MQ=255)
                   ccAGGAGTTTCAGGACAGTCTGAAAAAGGTTGAAAAGGCGAGCCTCACTAACCTGACGCCCGAACTGAAAGTGTCGATGGATGAACTACGCCa                                                                      >  1:5001742/1‑93 (MQ=255)
                     aGGAGTTTCAGGACAGTCTGAAAAAGGTTGAAAAGGCGAGCCTCACTAACCTGACGCCCGAACTGAAAGTGTCGATGGATGAACTACGCCAgg                                                                    <  1:209533/93‑1 (MQ=255)
                     aGGAGTTTCAGGACAGTCTGAAAAAGGTTGAAAAGGCGAGCCTCACTAACCTGACGCCCGAACTGAAAGTGTCGATGGATGAACTACGCCAgg                                                                    >  1:3543690/1‑93 (MQ=255)
                         gTTTCAGGACAGTCTGAAAAAGGTTGAAAAGGCGAGCCTCACTAACCTGACGCCCGAACTGAAAGTGTCGATGGATGAACTACGCCAGGCcgc                                                                >  2:627668/1‑93 (MQ=255)
                          tttCAGGACAGTCTGAAAAAGGTTGAAAAGGCGAGCCTCACTAACCTGACGCCCGAACTGAAAGTGTCGATGGATGAACTACGCCAGGCcgcg                                                               >  2:1143196/1‑93 (MQ=255)
                          tttCAGGACAGTCTGAAAAAGGTTGAAAAGGCGAGCCTCACTAACCTGACGCCCGAACTGAAAGTGTCGATGGATGAACTACGCCAGGCcgcg                                                               <  1:5722857/93‑1 (MQ=255)
                            tCAGGACAGTCTGAAAAAGGTTGAAAAGGCGAGCCTCACTAACCTGACGCCCGAACTGAAAGTGTCGATGGATGAACTACGCCAGGCCGCGGa                                                             >  1:480463/1‑93 (MQ=255)
                             cAGGACAGTCTGAAAAAGGTTGAAAAGGCGAGCCTCACTAACCTGACGCCCGAACTGAAAGTGTCGATGGATGAACTACGCCAGGCCGCGGAg                                                            >  2:2904456/1‑93 (MQ=255)
                             cAGGACAGTCTGAAAAAGGTTGAAAAGGCGAGCCTCACTAACCTGACGCCCGAACTGAAAGTGTCGATGGATGAACTACGCCAGGCCGCGGAg                                                            <  2:4118744/93‑1 (MQ=255)
                             cAGGACAGTCTGAAAAAGGTTGAAAAGGCGAGCCTCACTAACCTGACGCCCGAACTGAAAGTGTCGATGGATGAACTACGCCAGGCCGCGGAg                                                            <  1:4669745/93‑1 (MQ=255)
                              aGGACAGTCTGAAAAAGGTTGAAAAGGCGAGCCTCACTACCCTGACGCCCGAACTGAAAGTGTCGATGGATGAACTACGCCAGGCCGCGGAGt                                                           <  2:4371291/93‑1 (MQ=255)
                              aGGACAGTCTGAAAAAGGTTGAAAAGGCGAGCCTCACTAACCTGACGCCCGAACTGAAAGTGTCGATGGATGAACTACGCCAGGCCGCGGAgc                                                           >  1:1434621/1‑92 (MQ=255)
                                gACAGTCTGAAAAAGGTTGAAAAGGCGAGCCTCACTAACCTGACGCCCGAACTGAAAGTGTCGATGGATGAACTACGCCAGGCCGCGGAGTCg                                                         <  1:1066595/93‑1 (MQ=255)
                                   aGTCTGAAAAAGGTTGAAAAGGCGAGCCTCACTAACCTGACGCCCGAACTGAAAGTGTCGATGGATGAACTACGCCAGGCCGCGGAGTCGATg                                                      >  2:4163825/1‑93 (MQ=255)
                                    gTCTGAAAAAGGTTGAAAAGGCGAGCCTCACTAACCTGACGCCCGAACTGAAAGTGTCGATGGATGAACTACGCCAGGCCGCGGAGTCGATGa                                                     <  1:370698/93‑1 (MQ=255)
                                    gTCTGAAAAAGGTTGAAAAGGCGAGCCTCACTAACCTGACGCCCGAACTGAAAGTGTCGATGGATGAACTACGCCAGGCCGCGGAGTCGATGa                                                     >  1:3193172/1‑93 (MQ=255)
                                    gTCTGAAAAAGGTTGAAAAGGCGAGCCTCACTAACCTGACGCCCGAACTGAAAGTGTCGATGGATGAACTACGCCAGGCCGCGGAGTCGATGa                                                     >  1:4553130/1‑93 (MQ=255)
                                    gTCTGAAAAAGGTTGAAAAGGCGAGCCTCACTAACCTGACGCCCGAACTGAAAGTGTCGATGGATGAACTACGCCAGGCCGCGGAGTCGATGa                                                     >  1:4155711/1‑93 (MQ=255)
                                     tCTGAAAAAGGTTGAAAAGGCGAGCCTCACTAACCTGACGCCCGAACTGAAAGTGTCGATGGATGAACTACGCCAGGCCGCGGAGTCGATGaa                                                    >  2:4269284/1‑93 (MQ=255)
                                     tCTGAAAAAGGTTGAAAAGGCGAGCCTCACTAACCTGACGCCCGAACTGAAAGAGTCGATGGATGAACTACGCCAGGCCACGGAGTCGATCaa                                                    >  2:3839711/1‑93 (MQ=255)
                                      cTGAAAAAGGTTGAAAAGGCGAGCCTCACTAACCTGACGCCCGAACTGAAAGTGTCGATGGATGAACTACGCCAGGCCGCGGAGTCGATGAAg                                                   >  2:4757944/1‑93 (MQ=255)
                                       tGAAAAAGTTTGAAAAGGCGAGCCTCACTAACCTGACGCCCGAACTGAAAGTGTCGATGGATGAACTACGCCAGGCCGCGGAGTCGATGAAGc                                                  <  2:1401067/93‑1 (MQ=255)
                                       tGAAAAAGGTTGAAAAGGCGAGCCTCACTAACCTGACGCCCGAACTGAAAGTGTCGATGGATGAACTACGCCAGGCCGCGGAGTCGATGAAGc                                                  <  2:3652024/93‑1 (MQ=255)
                                        gAAAAAGGTTGAAAAGGCGAGCCTCACTAACCTGACGCCCGAACTGAAAGTGTCGATGGATGAACTACGCCAGGCCGCGGAGTCGATGAAGCg                                                 >  2:245319/1‑93 (MQ=255)
                                          aaaaGGTTGAAAAGGCGAGCCTCACTAACCTGACGCCCGAACTGAAAGTGTCGATGGATGAACTACGCCAGGCCGCGGAGTCGATGAAGCGtt                                               <  2:4704867/93‑1 (MQ=255)
                                          aaaaGGTTGAAAAGGCGAGCCTCACTAACCTGACGCCCGAACTGAAAGTGTCGATGGATGAACTACGCCAGGCCGCGGAGTCGATGAAGCGtt                                               >  1:51385/1‑93 (MQ=255)
                                           aaaGGTTGAAAAGGCGAGCCTCACTAACCTGACGCCCGAACTGAAAGTGTCGATGGATGAACTACGCCAGGCCGCGGAGTCGATGAAGCGTTc                                              >  1:2228828/1‑93 (MQ=255)
                                              ggTTGAAAAGGCGAGCCTCACTAACCTGACGCCCGAACTGAAAGTGTCGATGGATGAACTACGCCAGGCCGCGGAGTCGATGAAGCGTTCCTa                                           >  2:3797700/1‑93 (MQ=255)
                                               gTTGAAAAGGCGAGCCTCACTAACCTGACGCCCGAACTGAAAGTGTCGATGGATGAACTACGCCAGGCCGCGGAGTCGATGAAGCGTTCCTAc                                          <  1:1400093/93‑1 (MQ=255)
                                                ttGAAAAGGCGAGCCTCACTAACCTGACGCCCGAACTGAAAGTGTCGATGGATGAACTACGCCAGGCCGCGGAGTCGATGAAGCGTTCCTACg                                         >  1:2347433/1‑93 (MQ=255)
                                                ttGAAAAGGCGAGCCTCACCAACCTGACGCCCGAACTGAAAGTGTCGATGGATGAACTACGCCAGGCCGCGGAGTCGATGAAGCGTTCCTACg                                         >  2:6122775/1‑93 (MQ=255)
                                                  gAAAAGGCGAGCCTCACTAACCTGACGCCCGAACTGAAAGTGTCGATGGATGAACTACGCCAGGCCGCGGAGTCGATGAAGCGTTCCTACGtt                                       >  2:686524/1‑93 (MQ=255)
                                                  gAAAAGGCGAGCCTCACTAACCTGACGCCCGAACTGAAAGTGTCGATGGATGAACTACGCCAGGCCGCGGAGTCGATGAAGCGTTCCTACGtt                                       >  1:1022402/1‑93 (MQ=255)
                                                  gAAAACGCGAGCCTCACTAACCTGACGCCCGAACTGAAAGTGTCGATGGATGAACTACGCCAGGCCGCGGAGTCGATGAAGCGTTCCTACGtt                                       <  2:2141341/93‑1 (MQ=255)
                                                    aaaGGCGAGCCTCACTAACCTGACGCCCGAACTGAAAGTGTCGATGGATGAACTACGCCAGGCCGCGGAGTCGATGAAGCGTTCCTACGTTGc                                     <  2:2026931/93‑1 (MQ=255)
                                                    aaaGGCGAGCCTCACTAACCTGACGCCCGAACTGAAAGTGTCGATGGATGAACTACGCCAGGCCGCGGAGTCGATGAAGCGTTCCTACGTTGc                                     <  2:2626878/93‑1 (MQ=255)
                                                     aaGGCGAGCCTCACTAACCTGACGCCCGAACTGAAAGTGTCGATGGATGAACTACGCCAGGCCGCGGAGTCGATGAAGCGTTCCTACGTTGCa                                    <  1:1445243/93‑1 (MQ=255)
                                                       ggCGAGCCTCACTAACCTGACGCCCGAACTGAAAGTGTCGATGGATGAACTACGCCAGGCCGCGGAGTCGATGAAGCGTTCCTACGTTGCaaa                                  <  2:4570064/93‑1 (MQ=255)
                                                       ggCGAGCCTCACTAACCTGACGCCCGAACTGAAAGTGTCGATGGATGAACTACGCCAGGCCGCGGAGTCGATGAAGCGTTCCTACGTTGCaaa                                  <  1:1850443/93‑1 (MQ=255)
                                                       ggCGAGCCTCACTAACCTGACGCCCGAACTGAAAGTGTCGATGGATGAACTACGCCAGGCCGCGGAGTCGATGAAGCGTTCCTACGTTGCaaa                                  <  1:3873109/93‑1 (MQ=255)
                                                          gAGCCTCACTAACCTGACGCCCGAACTGAAAGTGTCGATGGATGAACTACGCCAGGCCGCGGAGTCGATGAAGCGTTCCTACGTTGCAAACGa                               >  2:3323983/1‑93 (MQ=255)
                                                          gAGCCTCACTAACCTGACGCCCGAACTGAAAGTGTCGATGGATGAACTACGCCAGGCCGCGGAGTCGATGAAGCGTTCCTACGTTGCAAACGa                               <  2:1022445/93‑1 (MQ=255)
                                                          gAGCCTCACTAACCTGACGCCCGAACTGAAAGTGTCGATGGATGAACTACGCCAGGCCGCGGAGTCGATGAAGCGTTCCTACGTTGCAAACGa                               <  1:739615/93‑1 (MQ=255)
                                                              cTCACTAACCTGACGCCCGAACTGAAAGTGTCGATGGATGAACTACGCCAGGCCGCGGAGTCGATGAAGCGTTCCTACGTTGCAAACGATCCt                           >  1:3365294/1‑93 (MQ=255)
                                                              cTCACTAACCTGACGCCCGAACTGAAAGTGTCGATGGATGAACTACGCCAGGCCGCGGAGTCGATGAAGCGTTCCTACGTTGCAAACGATCCt                           >  1:916418/1‑93 (MQ=255)
                                                              cTCACTAACCTGACGCCCGAACTGAAAGTGTCGATGGATGAACTACGCCAGGCCGCGGAGTCGATGAAGCGTTCCTACGTTGCAAACGATCCt                           >  1:2752824/1‑93 (MQ=255)
                                                              cTCACTAACCTGACGCCCGAACTGAAAGTGTCGATGGATGAACTACGCCAGGCCGCGGAGTCGATGAAGCGTTCCTACGTTGCAAACGATCCt                           >  2:1179828/1‑93 (MQ=255)
                                                               tCACTAACCTGACGCCCGAACTGAAAGTGTCGATGGATGAACTACGCCAGGCCGCGGAGTCGATGAAGCGTTCCTACGTTGCAAACGATCCTg                          >  1:1582821/1‑93 (MQ=255)
                                                                cACTAACCTGACGCCCGAACTGAAAGTGTCGATGGATGAACTACGCCAGGCCGCGGAGTCGATGAAGCGTTCCTACGTTGCAAACGATCCTGa                         <  2:5888533/93‑1 (MQ=255)
                                                                 aCTAACCTGACGCCCGAACTGAAAGTGTCGATGGATGAACTACGCCAGGCCGCGGAGTCGATGAAGCGTTCCTACGTTGCAAACGATCCTGaa                        >  1:1970910/1‑93 (MQ=255)
                                                                   tAACCTGACGCCCGAACTGAAAGTGTCGATGGATGAACTACGCCAGGCCGCGGAGTCGATGAAGCGTTCCTACGTTGCAAACGATCCTGaaaa                      >  1:567580/1‑93 (MQ=255)
                                                                   tAACCTGACGCCCGAACTGAAAGTGTCGATGGATGAACTACGCCAGGCCGCGGAGTCGATGAAGCGTTCCTACGTTGCAAACGATCCTGaaaa                      <  1:6247431/93‑1 (MQ=255)
                                                                    aaCCTGACGCCCGAACTGAAAGTGTCGATGGATGAACTACGCCAGGCCGCGGAGTCGATGAAGCGTTCCTACGTTGCAAACGATCCTGAAAAg                     <  1:601379/93‑1 (MQ=255)
                                                                    aaCCTGACGCCCGAACTGAAAGTGTCGATGGATGAACTACGCCAGGCCGCGGAGTCGATGAAGCGTTCCTACGTTGCAAACGATCCTGAAAAg                     >  2:5721085/1‑93 (MQ=255)
                                                                      ccTGACGCCCGAACTGAAAGTGTCGATGGATGAACTACGCCAGGCCGCGGAGTCGATGAAGCGTTCCTACGTTGCAAACGATCCTGAAAAGgc                   <  2:5236769/93‑1 (MQ=255)
                                                                      ccTGACGCCCGAACTGAAAGTGTCGATGGATGAACTACGCCAGGCCGCGGAGTCGATGAAGCGTTCCTACGTTGCAAACGATCCTGAAAAGgc                   <  1:1799844/93‑1 (MQ=255)
                                                                      ccTGACGCCCGAACTGAAAGTGTCGATGGATGAACTACGCCAGCCCGCGGAGTCGATGAAGCGTTCCTACGTTGCAAACGATCCTGAAAAGgc                   <  1:780733/93‑1 (MQ=255)
                                                                         gACGCCCGAACTGAAAGTGTCGATGGATGAACTACGCCAGGCCGCGGAGTCGATGAAGCGTTCCTACGTTGCAAACGATCCTGAAAAGgcgag                <  2:4444639/93‑1 (MQ=255)
                                                                         gACGCCCGAACTGAAAGTGTCGATGGATGAACTACGCCAGGCCGCGGAGTCGATGAAGCGTTCCTACGTTGCAAACGATCCTGAAAAGgcgag                >  1:5094513/1‑93 (MQ=255)
                                                                         gACGCCCGAACTGAAAGTGTCGATGGATGAACTACGCCAGGCCGCGGAGTCGATGAAGCGTTCCTACGTTGCAAACGATCCTGAAAAGgcgag                <  1:5549512/93‑1 (MQ=255)
                                                                         gACGCCCGAACTGAAAGTGTCGATGGATGAACTACGCCAGGCCGCGGAGTCGATGAAGCGTTCCTACGTTGCAAACGATCCTGAAAAGgcgag                >  1:1467352/1‑93 (MQ=255)
                                                                          aCGCCCGAACTGAAAGTGTCGATGGATGAACTACGCCAGGCCGCGGAGTCGATGAAGCGTTCCTACGTTGCAAACGATCCTGAAAAGgcgagc               >  2:2982391/1‑93 (MQ=255)
                                                                           cGCCCGAACTGAAAGTGTCGATGGATGAACTACGCCAGGCCGCGGAGTCGATGAAGCGTTCCTACGTTGCAAACGATCCTGAAAAGgcgagcg              >  2:4072635/1‑93 (MQ=255)
                                                                             cccGAACTGAAAGTGTCGATGGATGAACTACGCCAGGCCGCGGAGTCGATGAAGCGTTCCTACGTTGCAAACGATCCTGAAAAGgcgagcgag            >  1:4400056/1‑92 (MQ=255)
                                                                             cccGAACTGAAAGTGTCGATGGATGAACTACGCCAGGCCGCGGAGTCGATGAAGCGTTCCTACGTTGCAAACGATCCTGAAAAGGCGAGCGAt            >  1:4267494/1‑93 (MQ=255)
                                                                              ccGAACTGAAAGTGTCGATGGATGAACTACGCCAGGCCGCGGAGTCGATGAAGCGTTCCTACGTTGCAAACGATCCTGAAAAGGCGAGCGATg           >  1:2559662/1‑93 (MQ=255)
                                                                              ccGAACTGAAAGTGTCGATGGATGAACTACGCCAGGCCGCGGAGTCGATGAAGCGTTCCTACGTTGCAAACGATCCTGAAAAGGCGAGCGATg           <  2:5786477/93‑1 (MQ=255)
                                                                              ccGAACTGAAAGTGTCGATGGATGAACTACGCCAGGCCGCGGAGTCGATGAAGAGTTCCTACGTTGCAAACGATCCTGAAAAGGCGAGCGATg           >  2:5586592/1‑93 (MQ=255)
                                                                               cGAACTGAAAGTGTCGATGGATGAACTACGCCAGGCCGCGGAGTCGATGAAGCGTTCCTACGTTGCAAACGATCCTGAAAAGGCGAGCGATGa          <  2:4979681/93‑1 (MQ=255)
                                                                               cGAACTGAAAGTGTCGATGGATGAACTACGCCAGGCCGCGGAGTCGATGAAGCGTTCCTACGTTGCAAACGATCCTGAAAAGGCGAGCGATGa          <  2:5153846/93‑1 (MQ=255)
                                                                                gAACTGAAAGTGTCGATGGATGAACTACGCCAGGCCGCGGAGTCGATGAAGCGTTCCTACGTTGCAAACGATCCTGAAAAGGCGAGCGATGaa         >  1:4514868/1‑93 (MQ=255)
                                                                                gAACTGAAAGTGTCGATGGATGAACTACGCCAGGCCGCGGAGTCGATGAAGCGTTCCTACGTTGCAAACGATCCTGAAAAGGCGAGCGATGaa         <  2:430284/93‑1 (MQ=255)
                                                                                gAACTGAAAGTGTCGATGGATGAACTACGCCAGGCCGCGGAGTCGATGAAGCGTTCCTACGTTGCAAACGATCCTGAAAAGGCGAGCGATGaa         >  2:5390250/1‑93 (MQ=255)
                                                                                gAACTGAAAGTGTCGATGGATGAACTACGCCAGGCCGCGGAGTCGATGAAGCGTTCCTACGTTGCAAACGATCCTGAAAAGGCGAGCGATGaa         <  1:5875340/93‑1 (MQ=255)
                                                                                   cTGAAAGTGTCGATGGATGAACTACGCCAGGCCGCGGAGTCGATGAAGCGTTCCTACGTTGCAAACGATCCTGAAAAGGCGAGCGATGAAgcg      >  1:2961206/1‑93 (MQ=255)
                                                                                    tGAAAGTGTCGATGGATGAACTACGCCAGGCCGCGGAGTCGATGAAGCGTTCCTACGTTGCAAACGATCCTGAAAAGGCGAGCGATGAAgcgc     >  1:4856672/1‑93 (MQ=255)
                                                                                     gAAAGTGTCGATGGATGAACTACGCCAGGCCGCGGAGTCGATGAAGCGTTCCTACGTTGCAAACGATCCTGAAAAGGCGAGCGATGAAGCGca    >  1:673088/1‑93 (MQ=255)
                                                                                     gAAAGTGTCGATGGATGAACTACGCCAGGCCGCGGAGTCGATGAAGCGTTCCTACGTTGCAAACGATCCTGAAAAGGCGAGCGATGAAGCGca    >  2:2734830/1‑93 (MQ=255)
                                                                                     gAAAGTGTCGATGGATGAACTACGCCAGGCCGCGGAGTCGATGAAGCGTTCCTACGTTGCAAACGATCCTGAAAAGGCGAGCGATGAAGCGca    <  1:5205306/93‑1 (MQ=255)
                                                                                      aaaGTGTCGATGGATGAACTACGCCAGGCCGCGGAGTCGATGAAGCGTTCCTACGTTGCAAACGATCCTGAAAAGGCGAGCGATGAAGCGcac   >  2:3219877/1‑93 (MQ=255)
                                                                                      aaaGTGTCGATGGATGAACTACGCCAGGCCGCGGAGTCGATGAAGCGTTCCTACGTTGCAAACGATCCTGAAAAGGCGAGCGATGAAGCGcac   >  1:4485350/1‑93 (MQ=255)
                                                                                       aaGTGTCGATGGATGAACTACGCCAGGCCGCGGAGTCGATGAAGCGTTCCTACGTTGCAAACGATCCTGAAAAGGCGAGCGATGAAGCGcaca  >  2:2283195/1‑93 (MQ=255)
                                                                                          |                                                                                         
TGACCCAGGAGTTAAAACTCCAGGAGTTTCAGGACAGTCTGAAAAAGGTTGAAAAGGCGAGCCTCACTAACCTGACGCCCGAACTGAAAGCGTCGATGGATGAACTACGCCAGGCCGCGGAGTCGATGAAGCGTTCCTACGTTGCAAACGATCCTGAAAAGGCGAGCGATGAAGCGCACA  >  NC_000913/4019955‑4020134

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CTGACCCAGGAGTTAAAACTCCAGGAGTTTCAGGACAGTCTGAAAAAGGTTGAAAAGGCGAGCCTCACTAACCTGACGCCCGAACTGAAAGCGTCGATGGATGAACTACGCCAGGCCGCGGAGTCGATGAAGCGTTCCTACGTTGCAAACGATCCTGAAAAGGCGAGCGATGAAGCGCACACCAT  >  NC_000913/4019954‑4020138
                                                                                           |                                                                                             
CTGACCCAGGAGTTAAAACTCCAGGAGTTTCAGGACAGTCTGAAAAAGGTTGAAAAGGCGAGCCTCACTAACCTGACGCCCGAACTGAAAGTGT                                                                                             <  SRR4302533.2412150/94‑1 (MQ=60)
 TGGCCCAGGAGTTAAAACTCCAGGAGTTTCAGGACAGTCTGAAAAAGGTTGAAAAGGCGAGCCTCGCTAACCTGACGCCCGAACTGAAAGTGTC                                                                                            <  SRR4302533.1395811/94‑1 (MQ=60)
   ACCCAGGAGTTAAAACTCCAGGAGTTTCAGGACAGTCTGAAAAAGGTTGAAAAGGCGAGCCTCACTAACCTGACGCCCGAACTGAAAGTGTCGA                                                                                          >  SRR4302533.5562062/1‑94 (MQ=60)
     CCAGGAGTTAAAACTCCAGGAGTTTCAGGACAGTCTGAAAAAGGTTGAAAAGGCGAGCCTCACTAACCTGACGCCCGAACTGAAAGTGTCGATG                                                                                        <  SRR4302533.4368787/94‑1 (MQ=60)
     CCAGGAGTTAAAACTCCAGGAGTTTCAGGACAGTCTGAAAAAGGTTGAAAAGGCGAGCCTCACTAACCTGACGCCCGAACTGAAAGTGTCGATG                                                                                        <  SRR4302533.3431138/94‑1 (MQ=60)
     CCAGGAGTTAAAACTCCAGGAGTTTCAGGACAGTCTGAAAAAGGTTGAAAAGGCGAGCCTCACTAACCTGACGCCCGAACTGAAAGTGTCGATG                                                                                        >  SRR4302533.132795/1‑94 (MQ=60)
      CAGGAGTTAAAACTCCAGGAGTTTCAGGACAGTCTGAAAAAGGTTGAAAAGGCGAGCCTCACTAACCTGACGCCCGAACTGAAAGTGTCGATGG                                                                                       >  SRR4302533.5577943/1‑94 (MQ=60)
      CAGGAGTTAAAACTCCAGGAGTTTCAGGACAGTCTGAAAAAGGTTGAAAAGGCGAGCCTCACTAACCTGACGCCCGAACTGAAAGTGTCGATGG                                                                                       >  SRR4302533.1255152/1‑94 (MQ=60)
      CAGGAGTTAAAACTCCAGGAGTTTCAGGACAGTCTGAAAAAGGTTGAAAAGGCGAGCCTCACTAACCTGACGCCCGAACTGAAAGTGTCGATGG                                                                                       <  SRR4302533.5451396/94‑1 (MQ=60)
       AGGAGTTAAAACTCCAGGAGTTTCAGGACAGTCTGAAAAAGGTTGAAAAGGCGAGCCTCACTAACCTGACGCCCGAACTGAAAGTGTCGATGGA                                                                                      >  SRR4302533.5129437/1‑94 (MQ=60)
       AGGAGTTAAAACTCCAGGAGTTTCAGGACAGTCTGAAAAAGGTTGAAAAGGCGAGCCTCACTAACCTGACGCCCGAACTGAAAGTGTCGATGGA                                                                                      >  SRR4302533.3617656/1‑94 (MQ=60)
        GGATTTAAAACTCCGTGAGTTTCAGGACAGTCTGAAAAAGGTTGAAAAGGCGAGCCTCACTAACCTGACGCCCGAACTGAAAGTGTCGATGGAT                                                                                     <  SRR4302533.5540278/94‑1 (MQ=60)
         GAGTTAAAACTCCAGGAGTTTCAGGACAGTCTGAAAAAGGTTGAAAAGGCGAGCCTCACTAACCTGACGCCCGAACTGAAAGTGTCGATGGATG                                                                                    <  SRR4302533.1152446/94‑1 (MQ=60)
          AGTTAAAACTCCAGGAGTTTCAGGACAGTCTGAAAAAGGTTGAAAAGGCGAGCCTCACTAACCTGACGCCCGAACTGAAAGTGTCGATGGATGA                                                                                   >  SRR4302533.3942695/1‑94 (MQ=60)
              AAAACTCCAGGAGTTTCAGGACAGTCTGAAAAAGGTTGAAAAGGCGAGCCTCACTAACCTGACGCCCGAACTGAAAGTGTCGATGGATGAACTA                                                                               >  SRR4302533.5580603/1‑94 (MQ=60)
                AACTCCAGGAGTTTCAGGACAGTCTGAAAAAGGTTGAAAAGGCGAGCCTCACTAACCTGACGCCCGAACTGAAAGTGTCGATGGATGAACTACG                                                                             <  SRR4302533.1237854/94‑1 (MQ=60)
                AACTCCAGGAGTTTCAGGACAGTCTGAAAAAGGTTGAAAAGGCGAGCCTCACTAACCTGACGCCCGAACTGAAAGTGTCGATGGATGAACTACG                                                                             <  SRR4302533.3169222/94‑1 (MQ=60)
                  CTCCAGGAGTTTTAGGACAGTCTGAAAAAGGTTGAAAAGGCGAGCCTCACTAACCTGACGCCCGAACTGAAAGTGTCGATGGATGAACTACGCC                                                                           <  SRR4302533.5752374/94‑1 (MQ=60)
                  CTCCAGGAGTTTCAGGACAGTCTGAAAAAGGTTGAAAAGGCGAGCCTCACTAACCTGACGCCCGCACTGAAAGTGTCGATGGATGAACTACGCC                                                                           >  SRR4302533.4155114/1‑94 (MQ=60)
                   TCCAGGAGTTTCAGGACAGTCTGAAAAAGGTTGAAAAGGCGAGCCTCACTAACCTGACGCCCGAACTGAAAGTGTCGATGGATGAACTACGCCA                                                                          <  SRR4302533.4693532/94‑1 (MQ=60)
                   TCCAGGAGTTTCAGGACAGTCTGAAAAAGGTTGAAAAGGCGAGCCTCACTAACCTGACGCCCGAACTGAAAGTGTCGATGGATGAACTACGCCA                                                                          >  SRR4302533.5032062/1‑94 (MQ=60)
                    CCAGGAGTTTCAGGACAGTCTGAAAAAGGTTGAAAAGGCGAGCCTCACTAACCTGACGCCCGAACTGAAAGTGTCGATGGATGAACTACGCCAG                                                                         <  SRR4302533.2908366/94‑1 (MQ=60)
                    CCAGGAGTTTCAGGACAGTCTGAAAAAGGTTGAAAAGGCGAGCCTCACTAACCTGACGCCCGAACTGAAAGTGTCGATGGATGAACTACGCCAG                                                                         <  SRR4302533.6313071/94‑1 (MQ=60)
                     CAGGAGTTTCAGGACAGTCTGAAAAAGGTTGAAAAGGCGAGCCTCACTAACCTGACGCCCGAACTGAAAGTGTCGATGGATGAACTACGCCAGG                                                                        <  SRR4302533.4455663/94‑1 (MQ=60)
                     CAGGAGTTTCAGGACAGTCTGAAAAAGGTTGAAAAGGCGAGCCTCACTAACCTGACGCCCGAACTGAAAGTGTCGATGGATGAACTACGCCAGG                                                                        >  SRR4302533.3565529/1‑94 (MQ=60)
                     CAGGAGTTTCAGGACAGTCTGAAAAAGGTTGAAAAGGCGAGCCTCACTAACCTGACGCCCGAACTGAAAGTGTCGATGGATGAACTACGCCAGG                                                                        >  SRR4302533.1050510/1‑94 (MQ=60)
                     CAGGAGTTTCAGGACAGTCTGAAAAAGATTGAAAAGGCGAGCCTCACTAACCTGACGCCCGAACTGAAAGTGTCGATGGATGAACTACGCCAGG                                                                        >  SRR4302533.2475193/1‑94 (MQ=60)
                      AGGAGTTTCAGGACAGTCTGAAAAAGGTTGAAAAGGCGAGCCTCACTAACCTGACGCCCGAACTGAAAGTGTCGATGGATGAACTACGCCAGGC                                                                       >  SRR4302533.856221/1‑94 (MQ=60)
                      AGGAGTTTCAGGACAGTCTGAAAAAGGTTGAAAAGGCGAGCCTCACTAACCTGACGCCCGAACTGAAAGTGTCGATGGATGAACTACGCCAGGC                                                                       <  SRR4302533.210858/94‑1 (MQ=60)
                       GGAGTTTCAGGACAGTCTGAAAAAGGTTGAAAAGGCGAGCCTCACTAACCTGACGCCCGAACTGAAAGTGTCGATGGATGAACTACGCCAGGCC                                                                      <  SRR4302533.1987867/94‑1 (MQ=60)
                       GGAGTTTCAGGACAGTCTGAAAAAGGTTGAAAAGGCGAGCCTCACTAACCTGACGCCCGAACTGAAAGTGTCGATGGATGAACTACGCCAGGCC                                                                      <  SRR4302533.1570292/94‑1 (MQ=60)
                       GGAGTTGCAGGACAGTCTGAAAAAGGTTGAAAAGGCGAGCCTCACTAACCTGACGCCCGAACTGAAAGTGTCGATGGATGAACTACGCCAGGCC                                                                      <  SRR4302533.691773/94‑1 (MQ=60)
                        GAGTTTCAGGACAGTCTGAAAAAGGTTGAAAAGGCGAGCCTCACTAACCTGACGCCCGAACTGAAAGTGTCGATGGATGAACTACGCCAGGCCG                                                                     <  SRR4302533.3911310/94‑1 (MQ=60)
                         AGTTTCAGGACAGTCTGAAAAAGGTTGAAAAGGCGAGCCTCACTAACCTGACGCCCGAACTGAAAGTGTCGATGGATGAACTACGCCAGGCCGC                                                                    >  SRR4302533.954356/1‑94 (MQ=60)
                           TTTCAGGACAGTCTGAAAAAGGTTGAAAAGGCGAGCCTCACTAACCTGACGCCCGAACTGAAAGTGTCGATGGATGAACTACGCCAGGCCGCGG                                                                  <  SRR4302533.5757410/94‑1 (MQ=60)
                            TTCAGGACAGTCTGAAAAAGGTTGAAAAGGCGAGCCTCACTAACCTGACGCCCGAACTGAAAGTGTCGATGGATGAACTACGCCAGGCCGCGGA                                                                 >  SRR4302533.483335/1‑94 (MQ=60)
                              CAGGACAGTCTGAAAAAGGTTGAAAAGGCGAGCCTCACTAACCTGACGCCCGAACTGAAAGTGTCGATGGATGAACTACGCCAGGCCGCGGAGT                                                               <  SRR4302533.1436576/94‑1 (MQ=60)
                              CAGGACAGTCTGAAAAAGGTTGAAAAGGCGAGCCTCACTAACCTGACGCCCGAACTGAAAGTGTCGATGGATGAACTACGCCAGGCCGCGGAGT                                                               <  SRR4302533.4698043/94‑1 (MQ=60)
                              CAGGACAGTCTGAAAAAGGTTGAAAAGGCGAGCCTCACTAACCTGACGCCCGAACTGAAAGTGTCGATGGATGAACTACGCCAGGCCGCGGAGC                                                               >  SRR4302533.1443282/1‑94 (MQ=60)
                               AGGACAGTCTGAAAAAGGTTGAAAAGGCGAGCCTCACTAACCTGACGCCCGAACTGAAAGTGTCGATGGATGAACTACGCCAGGCCGCGGAGTC                                                              >  SRR4302533.2647463/1‑94 (MQ=60)
                                GGACAGTCTGAAAAAGTTTGAAAAGGCGAGCCTCACTAACCTGACGCCCGAACTGAAAGTGTCGATGGATGAACTACGCCAGGCCGCGGAGTCG                                                             <  SRR4302533.5059920/94‑1 (MQ=60)
                                GGACAGTCTGAAAAAGGTTGAAAAGGCGAGCCTCACTAACCTGACGCCCGAACTGAAAGTGTCGATGGATGAACTACGCCAGGCCGCGGAGTCG                                                             >  SRR4302533.2196495/1‑94 (MQ=60)
                                GGACAGTCTGAAAAAGGTTGAAAAGGCGAGCCTCACTAACCTGACGCCCGAACTGAAAGTGTCGATGGATGAACTACGCCAGGCCGCGGAGTCG                                                             <  SRR4302533.78061/94‑1 (MQ=60)
                                GGACAGTCTGAAAAAGGTTGAAAAGGCGAGCCTCACTAACCTGACGCCCGAACTGAAAGTGTCGATGGATGAACTACGCCAGGCCGCGGAGTCG                                                             <  SRR4302533.4826995/94‑1 (MQ=60)
                                 GACAGTCTGAAAAAGGTTGAAAAGGCGAGCCTCACTAACCTGACGCCCGAACTGAAAGTGTCGATGGATGAACTACGCCAGGCCGCGGAGTCGA                                                            >  SRR4302533.130203/1‑94 (MQ=60)
                                 GACAGTCTGAAAAAGGTTGAAAAGGCGAGCCTCACTAACCTGACGCCCGAACTGAAAGTGTCGATGGATGAACTACGCCAGGCCGCGGAGTCGA                                                            <  SRR4302533.1072862/94‑1 (MQ=60)
                                 GACAGTCTGAAAAAGGTTGAAAAGGCGAGCCTCACTAACCTGAAGCCCGAACTGAAAGTGTCGATGGATGAACTACGCCAGGCCGCGGAGTCGA                                                            >  SRR4302533.4468787/1‑94 (MQ=60)
                                  ACAGTCTGAAAAAGGTTGAAAAGGCGAGCCTCACTAACCTGCCGCCAGAACTGAAAGTGTCGATGGATGAACTACGCCAGGCCGCGGAGTCGAT                                                           <  SRR4302533.898617/94‑1 (MQ=60)
                                  ACAGTCTGAAAAAGGTTGAAAAGGCGAGCCTCACTAACCTGACGCCCGAACTGAAAGTGTCGATGGATGAACTACGCCAGGCCGCGGAGTCGAT                                                           <  SRR4302533.4522358/94‑1 (MQ=60)
                                    AGTCTGAAAAAGGTTGAAAAGGCGAGCCTCACTAACCTGACGCCCGAACTGAAAGTGTCGATGGATGAACTACGCCAGGCCGCGGAGTCGATGA                                                         >  SRR4302533.3212814/1‑94 (MQ=60)
                                    AGTCTGAAAAAGGTTGAAAAGGCGAGCCTCACTAACCTGACGCCCGAACTGAAAGTGTCGATGGATGAACTACGCCAGGCCGCGGAGTCGATGA                                                         >  SRR4302533.4180891/1‑94 (MQ=60)
                                    AGTCTGAAAAAGGTTGAAAAGGCGAGCCTCACTAACCTGACGCCCGAACTGAAAGTGTCGATGGATGAACTACGCCAGGCCGCGGAGTCGATGA                                                         >  SRR4302533.4580745/1‑94 (MQ=60)
                                    AGTCTGAAAAAGGTTGAAAAGGCGAGCCTCACTAACCTGACGCCCGAACTGAAAGTGTCGATGGATGAACTACGCCAGGCCGCGGAGTCGATGA                                                         >  SRR4302533.6064789/1‑94 (MQ=60)
                                     GTCTGAAAAAGGTTGAAAAGGCGAGCCTCACTAACCTGACGCCCGAACTGAAAGTGTCGATGGATGAACTACGCCAGGCCGCGGAGTCGATGAA                                                        >  SRR4302533.1259811/1‑94 (MQ=60)
                                     GTCTGAAAAAGGTTGAAAAGGCGAGCCTCACTAACCTGACGCCCGAACTGAAAGTGTCGATGGATGAACTACGCCAGGCCGCGGAGTCGATGAA                                                        <  SRR4302533.372936/94‑1 (MQ=60)
                                        TGAAAAAGGTTGAAAAGGCGAGCCTCACTAACCTGACGCCCGAACTGAAAGTGTCGATGGATGAACTACGCCAGGCCGCGGAGTCGATGAAGCG                                                     <  SRR4302533.5178947/94‑1 (MQ=60)
                                          AAAAAGGTTGAAAAGGCGAGCCTCACTAACCTGACGCCCGAACTGAAAGTGTCGATGGATGAACTACGCCAGGCCGCGGAGTCGATGAAGCGTT                                                   <  SRR4302533.493320/94‑1 (MQ=60)
                                          AAAAAGGTTGAAAAGGCGAGCCTCACTAACCTGACGCCCGAACTGAAAGTGTCGATGGATGAACTACGCCAGGCCGCGGAGTCGATGAAGCGTT                                                   >  SRR4302533.3894886/1‑94 (MQ=60)
                                          AAAAAGGTTGAAAAGGCGAGCCTCACTAACCTGACGCCCGAACTGAAAGTGTCGATGGATGAACTACGCCAGGCCGCGGAGTCGATGAAGCGTT                                                   >  SRR4302533.51733/1‑94 (MQ=60)
                                           AAAAGGTTGAAAAGGCGAGCCTCACTAACCTGACGCCCGAACTGAAAGTGTCGATGGATGAACTACGCCAGGCCGCGGAGTCGATGAAGCGTTC                                                  <  SRR4302533.744691/94‑1 (MQ=60)
                                           AAAAGGTTGAAAAGGCGAGCCTCACTAACCTGACGCCCGAACTGAAAGTGTCGATGGATGAACTACGCCAGGCCGCGGAGTCGATGAAGCGTTC                                                  >  SRR4302533.2242584/1‑94 (MQ=60)
                                            AAAGGTTGAAAAGGCGAGCCTCACTAACCTGACGCCCGAACTGAAAGTGTCGATGGATGAACTACGCCAGGCCGCGGAGTCGATGAAGCGTTCC                                                 >  SRR4302533.4557399/1‑94 (MQ=60)
                                            AAAGGTTGAAAAGGCGAGCCTCACTAACCTGACGCCCGAACTGAAAGTGTCGATGGATGAACTACGCCAGGCCGCGGAGTCGATGAAGCGTTCC                                                 <  SRR4302533.5451300/94‑1 (MQ=60)
                                             AAGGTTGAAAAGGCGAGCCTCACTAACCTGACGCCCGAACTGAAAGTGTCGATGGATGAACTACGCCAGGCCGCGGAGTCGATGAAGCGTTCCT                                                <  SRR4302533.1948941/94‑1 (MQ=60)
                                             AAGGTTGAAAAGGCGAGCCTCACTAACCCGACGCCCGAACTGAAAGTGTCGATGGATGAACTACGCCAGGCCGCGGAGTCGATGAAGCGTTCCT                                                <  SRR4302533.3996466/94‑1 (MQ=60)
                                              AGGTTGAAAAGGCGAGCCTCACTAACCTGACGCCCGAACTGAAAGTGTCGATGGATGAACTACGCCAGGCCGCGGAGTCGATGAAGCGTTCCTA                                               >  SRR4302533.2758249/1‑94 (MQ=60)
                                               GGTTGAAAAGGCGAGCCTCACTAACCTGACGCCCGAACTGAAAGTGTCGATGGATGAACTACGCCAGGCCGCGGAGTCGATGAAGCGTTCCTAC                                              <  SRR4302533.4540935/94‑1 (MQ=60)
                                                GTTGAAAAGGCGAGCCTCACTAACCTGACGCCCGAACTGAAGGTGTCGATGGATGAACTACGCCAGGCCGCGGAGTCGATGAAGCGTTCCTACG                                             <  SRR4302533.1870810/94‑1 (MQ=60)
                                                GTTGAAAAGGCGAGCCTCACTAACCTGACGCCCGAACTGAAAGTGTCGATGGATGAACTACGCCAGGCCGCGGAGTCGATGAAGCGTTCCTACG                                             >  SRR4302533.2361957/1‑94 (MQ=60)
                                                GTTGAAAAGGCGAGCCTCACTAACCTGACGCCCGAACTGAAAGTGTCGATGGATGAACTACGCCAGGCCGCGGAGTCGATGAAGCGTTCCTACG                                             <  SRR4302533.1408532/94‑1 (MQ=60)
                                                GTTGAAAAGGCGAGCCTCACTAACCTGACGCCCGAACTGAAAGTGTCGATGGATGAACTACGCCAGGCCGCGGAGTCGATGAAGCGTTCCTACG                                             <  SRR4302533.6013733/94‑1 (MQ=60)
                                                  TGAAAAGGCGAGCCTCACTAACCTGACGCCCGAACTGAAAGTGTCGATGGATGAACTACGCCAGGCCGCGGAGTCGATGAAGCGTTCCTACGTT                                           >  SRR4302533.5174514/1‑94 (MQ=60)
                                                  TGAAAAGGCGAGCCTCACTAACCTGACGCCCGAACTGAAAGTGTCGATGGATGAACTACGCCAGGCCGCGGAGTCGATGAAGCGTTCCTACGTT                                           <  SRR4302533.1694147/94‑1 (MQ=60)
                                                  TGAAAAGGCGAGCCTCACTAACCTGACGCCCGAACTGAAAGTGTCGATGGATGAACTACGCCAGGCCGCGGAGTCGATGAAGCGTTCCTACGTT                                           >  SRR4302533.1028413/1‑94 (MQ=60)
                                                   GAAAAGGCGAGCCTCACTAACCTGACGCCCGAACTGAAAGTGTCGATGGATGAACTACGCCAGGCCGCGGAGTCGATGAAGCGTTCCTACGTTG                                          >  SRR4302533.503414/1‑94 (MQ=60)
                                                     AAAGGCGAGCCTCACTAACCTGACGCCCGAACTGAAAGTGTCGATGGATGAACTACGCCAGGCCGCGGAGTCGATGAAGCGTTCCTACGTTGCA                                        >  SRR4302533.5157617/1‑94 (MQ=60)
                                                      AAGGCGAGCCTCACTAACCTGACGCCCGAACTGAAAGTGTCGATGGATGAACTACGCCAGGCCGCGGAGTCGATGAAGCGTTCCTACGTTGCAA                                       <  SRR4302533.1453963/94‑1 (MQ=60)
                                                      AAGGCGAGCCTCACTAACCTGACGCCCGAACTGAAAGTGTCGATGGATGAACTACGCCAGGCCGCGGAGTCGATGAAGCGTTCCTACGTTGCAA                                       >  SRR4302533.1451279/1‑94 (MQ=60)
                                                        GGCGAGCCTCACTAACCTGACGCCCGAACTGAAAGTGTCGATGGATGAACTACGCCAGGCCGCGGAGTCGATGAAGCGTTCCTACGTTGCAAAC                                     <  SRR4302533.3896749/94‑1 (MQ=60)
                                                        GGCGAGCCTCACTAACCTGACGCCCGAACTGAAAGTGTCGATGGATGAACTACGCCAGGCCGCGGAGTCGATGAAGCGTTCCTACGTTGCAAAC                                     <  SRR4302533.1861728/94‑1 (MQ=60)
                                                         GCGAGCCTCACTAACCTGACGCCCGAACTGAAAGTGTCGATGGATGAACTACGCCAGGCCGCGGAGTCGATGAAGCGTTCCTACGTTGCAAACG                                    >  SRR4302533.6059183/1‑94 (MQ=60)
                                                          CGAGCCTCACTAACCTGACGCCCGAACTGAAAGTGTCGATGGATGAACTACGCCAGGCCGCGGAGTCGATGAAGCGTTCCTACGTTGCAAACGA                                   >  SRR4302533.1457085/1‑94 (MQ=60)
                                                          CGAGCCTCACTAACCTGACGCCCGAACTGAAAGTGTCGATGGATGAACTACGCCAGGCCGCGGAGTCGATGAAGCGTTCCTACGTTGCAAACGA                                   >  SRR4302533.4272299/1‑94 (MQ=60)
                                                          CGAGCCTCACTAACCTGACGCCCGAACTGAAAGTGTCGATGGATGAACTACGCCAGGCCGCGGAGTCGATGAAGCGTTCCTACGTTGCAAACGA                                   >  SRR4302533.2852217/1‑94 (MQ=60)
                                                           GAGCCTCACTAACCTGACGCCCGAACTGAAAGTGTCGATGGATGAACTACGCCAGGCCGCGGAGTCGATGAAGCGTTCCTACGTTGCAAACGAT                                  <  SRR4302533.743968/94‑1 (MQ=60)
                                                           GAGCCTCACTAACCTGACGCCCGAACTGAAAGTGTCGATGGATGAACTACGCCAGGCCGCGGAGTCGATGAAGCGTTCCTACGTTGCAAACGAT                                  >  SRR4302533.2000127/1‑94 (MQ=60)
                                                             GCCTCACTAACCTGACGCCCGAACTGAAAGTGTCGATGGATGAACTACGCCAGGCCGCGGAGTCGATGAAGCGTTCCTACGTTGCAAACGATCC                                <  SRR4302533.2217687/94‑1 (MQ=60)
                                                              CCTCACTAACCTGACGCCCGAACTGAAAGTGTCGATGGATGAACTACGCCAGGCCGCGGAGTCGATGAAGCGTTCCTACGTTGCAAACGATCCT                               >  SRR4302533.2769704/1‑94 (MQ=60)
                                                              CCTCACTAACCTGACGCCCGAACTGAAAGTGTCGATGGATGAACTACGCCAGGCCGCGGAGTCGATGAAGCGTTCCTACGTTGCAAACGATCCT                               <  SRR4302533.2349062/94‑1 (MQ=60)
                                                              CCTCACTAACCTGACGCCCGAACTGAAAGTGTCGATGGATGAACTACGCCAGGCCGCGGAGTCGATGAAGCGTTCCTACGTTGCAAACGATCCT                               >  SRR4302533.5652817/1‑94 (MQ=60)
                                                              CCTCACTAACCTGACGCCCGAACTGAAAGTGTCGATGGATGAACTACGCCAGGCCGCGGAGTCGATGAAGCGTTCCTACGTTGCAAACGATCCT                               >  SRR4302533.2912561/1‑94 (MQ=60)
                                                              CCTCACTAACCTGACGCCCGAACTGAAAGTGTCGATGGATGAACTACGCCAGGCCGCGGAGTCGATGAAGCGTTCCTACGTTGCAAACGATCCT                               <  SRR4302533.4309106/94‑1 (MQ=60)
                                                              CCTCACTAACCTGACGCCCGAACTGAAAGTGTCGATGGATGAACTACGCCAGGCCGCGGAGTCGATGAAGCGTTCCTACGTTGCAAACGATCCT                               >  SRR4302533.3386082/1‑94 (MQ=60)
                                                              CCTCACTAACCTGACGCCCGAACTGAAAGTGTCGATGGATGAACTACGCCAGGCCGCGGAGTCGATGAAGCGTTCCTACGTTGCAAACGATCCT                               <  SRR4302533.3512768/94‑1 (MQ=60)
                                                              CCTCACTAACCTGACGCCCGAACTGAAAGTGTCGATGGATGAACTACGCCAGGCCGCGGAGTCGATGAAGCGTTCCTACGTTGCAAACGATCCT                               >  SRR4302533.921802/1‑94 (MQ=60)
                                                               CTCACTAACCTGACGCCCGAACTGAAAGTGTCGATGGATGAACTACGCCAGGCCGCGGAGTCGATGAAGCGTTCCTACGTTGCAAACGATCCTG                              >  SRR4302533.1592359/1‑94 (MQ=60)
                                                               CTCACTAACCTGACGCCCGAACTGAAAGTGTCGATGGATGAACTACGCCAGGCCGCGGAGTCGATGAAGCGTTCCTACGTTGCAAACGATCCTG                              <  SRR4302533.983979/94‑1 (MQ=60)
                                                               CTCACTAACCTGACGCCCGAACTGAAAGTGTCGATGGATGAACTACGCCAGGCCGCGGAGTCGATGAAGCGTTCCTACGTTGCAAACGATCCTG                              >  SRR4302533.5711349/1‑94 (MQ=60)
                                                                TCACTAACCTGACGCCCGAACTGAAAGTGTCGATGGATGAACTACGCCAGGCCGCGGAGTCGATGAAGCGTTCCTACGTTGCAAACGATCCTGA                             <  SRR4302533.1817754/94‑1 (MQ=60)
                                                                TCACTAACCTGACGCCCGAACTGAAAGTGTCGATGGATGAACTACGCCAGGCCGCGGAGTCGATGAAGCGTTCCTACGTTGCAAACGATCCTGA                             >  SRR4302533.6046167/1‑94 (MQ=60)
                                                                TCACTAACCTGACGCCCGAACTGAAAGTGTCGATGGATGAACTACGCCAGGCCGCGGAGTCGATGAAGCGTTCCTACGTTGCAAACGATCCTGA                             <  SRR4302533.3418977/94‑1 (MQ=60)
                                                                TCACTAACCTGACGCCCGAACTGAAAGTGTCGATGGATGAACTACGCCAGGCCGCGGAGTCGATGAAGCGTTCCTACGTTGCAAACGATCCTGA                             >  SRR4302533.3106131/1‑94 (MQ=60)
                                                                 CACTAACCTGACGCCCGAACTGAAAGTGTCGATGGATGAACTACGCCAGGCCGCGGAGTCGATGAAGCGTTCCTACGTTGCAAACGATCCTGAA                            >  SRR4302533.1982969/1‑94 (MQ=60)
                                                                   CTAACCTGACGCCCGAACTGAAAGTGTCGATGGATGAACTACGCCAGGCCGCGGAGTCGATGAAGCGTTCCTACGTTGCAAACGATCCTGAAAA                          >  SRR4302533.5319773/1‑94 (MQ=60)
                                                                   CTAACCTGACGCCCGAACTGAAAGTGTCGATGGATGAACTACGCCAGGCCGCGGAGTCGATGAAGCGTTCCTACGTTGCAAACGATCCTGAAAA                          >  SRR4302533.570940/1‑94 (MQ=60)
                                                                   CTAACCTGACGCCCGAACTGAAAGTGTCGATGGATGAACTACGCCAGGACGCGGAGTCGATGAAGCGTTCCTACGTTGCAAACGATCCTGAAAA                          >  SRR4302533.37369/1‑94 (MQ=60)
                                                                    TAACCTGACGCCCGAACTGAAAGTGTCGATGGATGAACTACGCCAGGCCGCGGAGTCGATGAAGCGTTCCTACGTTGCAAACGATCCTGAAAAG                         <  SRR4302533.484920/94‑1 (MQ=60)
                                                                    TAACCTGACGCCCGAACTGAAAGTGTCGATGGATGAACTACGCCAGGCCGCGGAGTCGATGAAGCGTTCCTACGTTGCAAACGATCCTGAAAAG                         <  SRR4302533.6284817/94‑1 (MQ=60)
                                                                    TAACCTGACGCCCGAACTGAAAGTGTCGATGGATGAACTACGCCAGGCCGCGGAGTCGATGAAGCGTTCCTACGCTGCAAACGATCCTGAAAAG                         >  SRR4302533.566754/1‑94 (MQ=60)
                                                                     AACCTGACGCCCGAACTGAAAGTGTCGATGGATGAACTACGCCAGGCCGCGGAGTCGATGAAGCGTTCCTACGTTGCAAACGATCCTGAAAAGG                        <  SRR4302533.604915/94‑1 (MQ=60)
                                                                       CCTGACGCCCGAACTGAAAGTGTCGATGGATGAACTACGCCAGGCCGCGGAGTCGATGAAGCGTTCCTACGTTGCAAACGATCCTGAAAAGGCG                      >  SRR4302533.4690386/1‑94 (MQ=60)
                                                                       CCTGACGCCCGAACTGAAAGTGTCGATGGATGAACTACGCCAGGCCGCGGAGTCGATGAAGCGTTCCTACGTTGCAAACGATCCTGAAAAGGCG                      <  SRR4302533.1810793/94‑1 (MQ=60)
                                                                       CCTGACGCCCGAACTGAAAGTGTCGATGGATGAACTACGCCAGCCCGCGGAGTCGATGAAGCGTTCCTACGTTGCAAACGATCCTGAAAAGGCG                      <  SRR4302533.785333/94‑1 (MQ=60)
                                                                        CTGACGCCCGAACTGAAAGTGTCGATGGATGAACTACGCCAGGCCGCGGAGTCGATGAAGCGTTCCTACGTTGCAAACGATCCTGAAAAGGCGA                     <  SRR4302533.1821151/94‑1 (MQ=60)
                                                                         TGACGCCCGAACTGAAAGTGTCGATGGATGAACTACGCCAGGCCGCGGAGTCGATGAAGCGTTCCTACGTTGCAAACGATCCTGAAAAGGCGAG                    >  SRR4302533.1476200/1‑94 (MQ=60)
                                                                         TGACGCCCGAACTGAAAGTGTCGATGGATGAACTACGCCAGGCCGCGGAGTCGATGAAGCGTTCCTACGTTGCAAACGATCCTGAAAAGGCGAG                    >  SRR4302533.5125289/1‑94 (MQ=60)
                                                                         TGACGCCCGAACTGAAAGTGTCGATGGATGAACTACGCCAGGCCGCGGAGTCGATGAAGCGTTCCTACGTTGCAAACGATCCTAAAAAGGCGAG                    >  SRR4302533.3628605/1‑94 (MQ=60)
                                                                          GACGCCCGAACTGAAAGTGTCGATGGATGAACTACGCCAGGCCGCGGAGTCGATGAAGCGTTCCTACGTTGCAAACGATCCTGAAAAGGCGAGC                   <  SRR4302533.670007/94‑1 (MQ=60)
                                                                          GACGCCCGAACTGAAAGTGTCGATGGATGAACTACGCCAGGCCGCGGAGTCGATGAAGCGTTCCTACGTTGCAAACGATCCTGAAAAGGCGAGC                   <  SRR4302533.5583024/94‑1 (MQ=60)
                                                                          GACGCCCGAACTGAAAGTGTCGATGGATGAACTACGCCAGGCCGCGGAGTCGATGAAGCGTTCCTACGTTGCAAACGATCCTGAAAAGGCGAGC                   >  SRR4302533.585539/1‑94 (MQ=60)
                                                                            CGCCCGAACTGAAAGTGTCGATGGATGAACTACGCCAGGCCGCGGAGTCGATGAAGCGTTCCTACGTTGCAAACGATCCTGAAAAGGCGAGCGA                 >  SRR4302533.3332844/1‑94 (MQ=60)
                                                                            CGCCCGAACTGAAAGTGTCGATGGATGAACTACGCCAGGCCGCGGAGTCGATGAAGCGTTCCTACGTTGCAAACGATCCTGAAAAGGCGAGCGA                 >  SRR4302533.486182/1‑94 (MQ=60)
                                                                             GCCCGAACTGAAAGTGTCGATGGATGAACTACGCCAGGCCGCGGAGTCGATGAAGCGTTCCTACGTTGCAAACGATCCTGAAAAGGCGAGCGAT                >  SRR4302533.4293190/1‑94 (MQ=60)
                                                                             GCCCGAACTGAAAGTGTCGATGGATGAACTACGCCAGGCCGCGGAGTCGATGAAGCGTTCCTACGTTGCAAACGATCCTGAAAAGGCGAGCGAT                >  SRR4302533.3268042/1‑94 (MQ=60)
                                                                             GCCCGAACTGAAAGTGTCGATGGATGAACTACGCCAGGCCGCGGAGTCGATGAAGCGTTCCTACGTTGCAAACGATCCTGAAAAGGCGAGCGAG                >  SRR4302533.4426623/1‑94 (MQ=60)
                                                                              CCCGAACTGAAAGTGTCGATGGATGAACTACGCCAGGCCGCGGAGTCGATGAAGCGTTCCTACGTTGCAAACGATCCTGAAAAGGCGAGCGATG               >  SRR4302533.2575429/1‑94 (MQ=60)
                                                                              CCCGAACTGAAAGTGTCGATGGATGAACTACGCCAGGCCGCGGAGTCGATGAAGCGTTCCTACGTTGCAAACGATCCTGAAAAGGCGAGCGATG               >  SRR4302533.4725296/1‑94 (MQ=60)
                                                                               CCGAACTGAAAGTGTCGATGGATGAACTACGCCAGGCCGCGGAGTCGATGAAGCGTTCCTACGTTGCAAACGATCCTGAAAAGGCGAGCGATGA              >  SRR4302533.1518574/1‑94 (MQ=60)
                                                                                CGAACTGAAAGTGTCGATGGATGAACTACGCCAGGCCGCGGAGTCGATGAAGCGTTCCTACGTTGCAAACGATCCTGAAAAGGCGAGCGATGAA             >  SRR4302533.4542207/1‑94 (MQ=60)
                                                                                CGAACTGAAAGTGTCGATGGATGAACTACGCCAGGCCGCGGAGTCGATGAAGCGTTCCTACGTTGCAAACGATCCTGAAAAGGCGAGCGATGAA             >  SRR4302533.341105/1‑94 (MQ=60)
                                                                                 GAACTGAAAGTGTCGATGGATGAACTACGCCAGGCCGCGGAGTCGATGAAGCGTTCCTACGTTGCAAACGATCCTGAAAAGGCGAGCGATGAAG            <  SRR4302533.5910858/94‑1 (MQ=60)
                                                                                  AACTGAAAGTGTCGATGGATGAACTACGCCAGGCCGCGGAGTCGATGAAGCGTTCCTACGTTGCAAACGATCCTGAAAAGGCGAGCGATGAAGC           >  SRR4302533.1460787/1‑94 (MQ=60)
                                                                                   ACTGAAAGTGTCGATGGATGAACTACGCCAGGCCGCGGAGTCGATGAAGCGTTCCTACGTTGCAAACGATCCTGAAAAGGCGAGCGATGAAGCG          >  SRR4302533.2979309/1‑94 (MQ=60)
                                                                                    CTGAAAGTGTCGATGGATGAACTACGCCAGGCCGCGGAGTCGATGAAGCGTTCCTACGTTGCAAACGATCCTGAAAAGGCGAGCGATGAAGCGC         >  SRR4302533.4886105/1‑94 (MQ=60)
                                                                                    CTGAAAGTGTCGATGGATGAACTACGCCAGGCCGCGGAGTCGATGAAGCGTTCCTACGTTGCAAACGATCCTGAAAAGGCGAGCGATGAAGCGC         <  SRR4302533.1444541/94‑1 (MQ=60)
                                                                                     TGAAAGTGTCGATGGATGAACTACGCCAGGCCGCGGAGTCGATGAAGCGTTCCTACGTTGCAAACGATCCTGAAAAGGCGAGCGATGAAGCGCA        >  SRR4302533.677063/1‑94 (MQ=60)
                                                                                     TGAAAGTGTCGATGGATGAACTACGCCAGGCCGCGGAGTCGATGAAGCGTTCCTACGTTGCAAACGATCCTGAAAAGGCGAGCGATGAAGCGCA        >  SRR4302533.1349135/1‑94 (MQ=60)
                                                                                     TGAAAGTGTCGATGGATGAACTACGCCAGGCCGCGGAGGCGATGAAGCGTTCCTACGTTGCAAACGATCCTGAAAAGGCGAGCGATGAAGCGGA        >  SRR4302533.2369497/1‑94 (MQ=60)
                                                                                      GAAAGTGTCGATGGATGAACTACGCCAGGCCGCGGAGTCGATGAAGCGTTCCTACGTTGCAAACGATCCTGAAAAGGCGAGCGATGAAGCGCAC       <  SRR4302533.3918736/94‑1 (MQ=60)
                                                                                      GAAAGTGTCGATGGATGAACTACGCCAGGCCGCGGAGTCGATGAAGCGTTCCTACGTTGCAAACGATCCTGAAAAGGCGAGCGATGAAGCGCAC       <  SRR4302533.5236673/94‑1 (MQ=60)
                                                                                      GAAAGTGTCGATGGATGAACTACGCCAGGCCGCGGAGTCGATGAAGCGTTCCTACGTTGCAAACGATCCTGAAAAGGCGAGCGATGAAGCGCAC       >  SRR4302533.4512506/1‑94 (MQ=60)
                                                                                       AAAGTGTCGATGGATGAACTACGCCAGGCCGCGGAGTCGATGAAGCGTTCCTACGTTGCAAACGATCCTGAAAAGGCGAGCGATGAAGCGCACA      <  SRR4302533.2836747/94‑1 (MQ=60)
                                                                                         AGTGTCGATGGATGAACTACGCCAGGCCGCGGAGTCGATGAAGCGTTCCTACGTTGCAAACGATCCTGAAAAGGCGAGCGATGAAGCGCACACC    >  SRR4302533.3677359/1‑94 (MQ=60)
                                                                                          GTGTCGATGGATGAACTACGCCAGGCCGCGGAGTCGATGAAGCGTTCCTACGTTGCAAACGATCCTGAAAAGGCGAGCGATGAAGCGCACACCA   >  SRR4302533.5286647/1‑94 (MQ=60)
                                                                                           TGTCGATGGATGAACTACGCCAGGCCGCGGAGTCGATGAAGCGTTCCTACGTTGCAAACGATCCTGAAAAGGCGAGCGATGAAGCGCACACCAT  <  SRR4302533.1446935/94‑1 (MQ=60)
                                                                                           TGTCGATGGATGAACTACGCCAGGCCGCGGAGTCGATGAAGCGTTCCTACGTTGCAAACGATCCTGAAAAGGCGAGCGATGAAGCGCACACCAT  <  SRR4302533.4539408/94‑1 (MQ=60)
                                                                                           |                                                                                             
CTGACCCAGGAGTTAAAACTCCAGGAGTTTCAGGACAGTCTGAAAAAGGTTGAAAAGGCGAGCCTCACTAACCTGACGCCCGAACTGAAAGCGTCGATGGATGAACTACGCCAGGCCGCGGAGTCGATGAAGCGTTCCTACGTTGCAAACGATCCTGAAAAGGCGAGCGATGAAGCGCACACCAT  >  NC_000913/4019954‑4020138

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑