Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,056,373 A→G K129K (AAA→AAG)  yihK → putative GTP‑binding factor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,056,3730AG100.0% 831.5 / NA 258K129K (AAA→AAG) yihKputative GTP‑binding factor
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (115/143);  total (115/143)

Only 100 of 265 total aligned reads displayed.

TGACGCATTTGACGGCCCGATGCCGCAAACGCGCTTCGTAACCAAAAAAGCGTTTGCTTACGGCCTGAAGCCGATTGTTGTTATCAACAAAGTTGACCGCCCTGGCGCGCGTCCTGATTGGGTTGTGGATCAGGTATTCGATCTGTTCGTTAACCTCGACGCGACCGACGAGCAGCTGGA  >  NC_000913/4056283‑4056462
                                                                                          |                                                                                         
tGACGCATTTGACGGCCCGATGCCGCAAACGCGCTTCGTAACCAAAAAAGCGTTTGCTTACGGCCTGAAGCCGATTGTTGTTATCAACAAGGt                                                                                         >  1:4789197/1‑93 (MQ=255)
tGACGCATTTGACGGCCCGATGCCGCAAACGCGCTTCGTAACCAAAAAAGCGTTTGCTTACGGCCTGAAGCCGATTGTTGTTATCAACAAGGt                                                                                         >  1:2128024/1‑93 (MQ=255)
 gACGCATTTGACGGCCCGATGCCGCAAGCGCGCTTCGTAACCAAAAAAGCGTTTGCTTACGGCCTGAAGCCGATTGTTGTTATCAACAAGGtt                                                                                        <  2:3448445/93‑1 (MQ=255)
 gACGCATTTGACGGCCCGATGCCGCAAACGCGCTTCGTAACCAAAAAAGCGTTTGCTTACGGCCTGAAGCCGATTGTTGTTATCAACAAGGtt                                                                                        <  1:2953858/93‑1 (MQ=255)
 gACGCATTTGACGGCCCGATGCCGCAAACGCGCTTCGTAACCAAAAAAGCGTTTGCTTACGGCCTGAAGCCGATTGTTGTTATCAACAAGGtt                                                                                        <  2:1608437/93‑1 (MQ=255)
   cGCATTTGACGGCCCGATGCCGCAAACGCGCTTCGTAACCAAAAAAGCGTTTGCTTACGGCCTGAAGCCGATTGTTGTTATCAACAAGGTTGa                                                                                      <  2:1317563/93‑1 (MQ=255)
   cGCATTTGACGGCCCGATGCCGCAAACGCGCTTCGTAACCAAAAAAGCGTTTGCTTACGGCCTGAAGCCGATTGTTGTTATCAACAAGGTTGa                                                                                      <  1:3809117/93‑1 (MQ=255)
   cGCATTTGACGGCCCGATGCCGCAAACGCGCTTCGTAACCAAAAAAGCGTTTGCTTACGGCCTGAAGCCGATTGTTGTTATCAACAAGGTTGa                                                                                      <  2:2598736/93‑1 (MQ=255)
     cATTTGACGGCCCGATGCCGCAAACGCGCTTCGTAACCAAAAAAGCGTTTGCTTACGGCCTGAAGCCGATTGTTGTTATCAACAAGGTTGAcc                                                                                    >  2:4130845/1‑93 (MQ=255)
     cATTTGACGGCCCGATGCCGCAAACGCGCTTCGTAACCAAAAAAGCGTTTGCTTACGGCCTGAAGCCGATTGTTGTTATCAACAAGGTTGAcc                                                                                    >  1:2320781/1‑93 (MQ=255)
      atgtGACGGCCCGATGCCGCAAACGCGCTTCGTAACCAAAAAAGCGTTTGCTTACGGCCTGAAGCCGATTGTTGTTATCAACAAGGTTGACCg                                                                                   <  2:2908372/90‑1 (MQ=255)
      aTTTGACGGCCCGATGCCGCAAACGCGCTTCGTAACCAAAAAAGCGTTTGCTTACGGCCTGAAGCCGATTGTTGTTATCAACAAGGTTGACCg                                                                                   >  1:6137198/1‑93 (MQ=255)
       tttGACGGCCCGATGCCGCAAACGCGCTTCGTAACCAAAAAAGCGTTTGCTTACGGCCTGAAGCCGATTGTTGTTATCAACAAGGTTGACCGc                                                                                  >  2:189554/1‑93 (MQ=255)
         tGACGGCCCGATGCCGCAAACGCGCTTCGTAACCAAAAAAGCGTTTGCTTACGGCCTGAAGCCGATTGTTGTTATCAACAAGGTTGACCGccc                                                                                <  2:4857373/93‑1 (MQ=255)
          gACGGCCCGATGCCGCAAACGCGCTTCGTAACCAAAAAAGCGTTTGCTTACGGCCTGAAGCCGATTGTTGTTATCAACAAGGTTGACCGCCCt                                                                               <  1:2564623/93‑1 (MQ=255)
               cccGATGCCGCAAACGCGCTTCGTAACCAAAAAAGCGTTTGCTTACGGCCTGAAGCCGATTGTTGTTATCAACAAGGTTGACCGCCCTGgcgc                                                                          <  1:4890793/93‑1 (MQ=255)
               cccGATGCCGCAAACGCGCTTCGTAACCAAAAAAGCGTTTGCTTACGGCCTGAAGCCGATTGTTGTTATCAACAAGGTTGACCGCCCTGgcgc                                                                          >  2:5654834/1‑93 (MQ=255)
                ccGATGCCGCAAACGCGCTTCGTAACCAAAAAAGCGTTTGCTTACGGCCTGAAGCCGATTGTTGTTATCAACAAGGCTGATCGCCCTGgcgcg                                                                         >  2:3265155/1‑93 (MQ=255)
                 cGATGCCGCAAACGCGCTTCGTAACCAAAAAAGCGTTTGCTTACGGCCTGAAGCCGATTGTTGTTATCAACAAGGTTGACCGCCCTGgcgcgc                                                                        >  2:1129277/1‑93 (MQ=255)
                  gATGCCGCAAACGCGCTTCGTAACCAAAAAAGCGTTTGCTTACGGCCTGAAGCCGATTGTTGTTATCAACAAGGTTGACCGCCCTGGcgcgcg                                                                       >  1:3257741/1‑93 (MQ=255)
                  gATGCCGCAAACGCGCTTCGTAACCAAAAAAGCGTTTGCTTACGGCCTGAAGCCGATTGTTGTTATCAACAAGGTTGACCGCCCTGGcgcgcg                                                                       >  1:1190567/1‑93 (MQ=255)
                    tGCCGCAAACGCGCTTCGTAACCAAAAAAGCGTTTGCTTACGGCCTGAAGCCGATTGTTGTTATCAACAAGGTTGACCGCCCTGGCGCGCGTc                                                                     <  1:5663076/93‑1 (MQ=255)
                      ccGCAAACGCGCTTCGTAACCAAAAAAGCGTTTGCTTACGGCCTGAAGCCGATTGTTGTTATCAACAAGGTTGACCGCCCTGGCGCGCGTCCt                                                                   <  1:918051/93‑1 (MQ=255)
                      ccGCAAACGCGCTTCGTAACCAAAAAAGCGTTTGCTTACGGCCTGAAGCCGATTGTTGTTATCAACAAGGTTGACCGCCCTGGCGCGCGTCCt                                                                   <  1:5514887/93‑1 (MQ=255)
                       cGCAAACGCGCTTCGTAACCAAAAAAGCGTTTGCTTACGGCCTGAAGCCGATTGTTGTTATCAACAAGGTTGACCGCCCTGGCGCGCGTCCTg                                                                  <  2:1205616/93‑1 (MQ=255)
                       cGCAAACGCGCTTCGTAACCAAAAAAGCGTTTGCTTACGGCCTGAAGCCGATTGTTGTTATCAACAAGGTTGACCGCCCTGGCGCGCGTCCTg                                                                  <  1:4843163/93‑1 (MQ=255)
                       cGCAAACGCGCTTCGTAACCAAAAAAGCGTTTGCTTACGGCCTGAAGCCGATTGTTGTTATCAACAAGGTTGACCGCCCTGGCGCGCGTCCTg                                                                  >  1:4425076/1‑93 (MQ=255)
                        gCAAACGCGCTTCGTAACCAAAAAAGCGTTTGCTTACGGCCTGAAGCCGATTGTTGTTATCAACAAGGTTGACCGCCCTGGCGCGCGTCCTGa                                                                 <  2:779935/93‑1 (MQ=255)
                        gCAAACGCGCTTCGTAACCAAAAAAGCGTTTGCTTACGGCCTGAAGCCGATTGTTGTTATCAACAAGGTTGACCGCCCTGGCGCGCGTCCTGa                                                                 <  2:1383371/93‑1 (MQ=255)
                         cAAACGCGCTTCGTAACCAAAAAAGCGTTTGCTTACGGCCTGAAGCCGATTGTTGTTATCAACAAGGTTGACCGCCCTGGCGCGCGTCCTGAt                                                                <  2:5342381/93‑1 (MQ=255)
                          aaaCGCGCTTCGTAACCAAAAAAGCGTTTGCTTACGGCCTGAAGCCGATTGTTGTTATCAACAAGGTTGACCGCCCTGGCGCGCGTCCTGAtt                                                               >  2:2363898/1‑93 (MQ=255)
                          aaaCGCGCTTCGTAACCAAAAAAGCGTTTGCTTACGGCCTGAAGCCGATTGTTGTTATCAACAAGGTTGACCGCCCTGGCGCGCGTCCTGAtt                                                               >  1:1289694/1‑93 (MQ=255)
                           aaCGCGCTTCGTAACCAAAAAAGCGTTTGCTTACGGCCTGAAGCCGATTGTTGTTATCAACAAGGTTGACCGCCCTGGCGCGCGTCCTGATTg                                                              >  2:249849/1‑93 (MQ=255)
                            aCGCGCTTCGTAACCAAAAAAGCGTTTGCTTACGGCCTGAAGCCGATTGTTGTTATCAACAAGGTTGACCGCCCTGGCGCGCGTCCTGATTgg                                                             <  2:2943609/93‑1 (MQ=255)
                            aCGCGCTTCGTAACCAAAAAAGCGTTTGCTTACGGCCTGAAGCCGATTGTTGTTATCAACAAGGTTGACCGCCCTGGCGCGCGTCCTGATTgg                                                             >  2:1787168/1‑93 (MQ=255)
                            aCGCGCTTCGTAACCAAAAAAGCGTTTGCTTACGGCCTGAAGCCGATTGTTGTTATCAACAAGGTTGACCGCCCTGGCGCGCGTCCTGATTgg                                                             <  2:1614268/93‑1 (MQ=255)
                              gcgcTTCGTAACCAAAAAAGCGTTTGCTTACGGCCTGAAGCCGATTGTTGTTATCAACAAGGTTGACCGCCCTGGCGCGCGTCCTGATTGGGt                                                           >  1:1102794/1‑93 (MQ=255)
                              gcgcTTCGTAACCAAAAAAGCGTTTGCTTACGGCCTGAAGCCGATTGTTGTTATCAACAAGGTTGACCGCCCTGGCGCGCGTCCTGATTGGGt                                                           <  1:5736084/93‑1 (MQ=255)
                               cgcTTCGTAACCAAAAAAGCGTTTGCTTACGGCCTGAAGCCGATTGTTGTTATCAACAAGGTTGACCGCCCTGGCGCGCGTCCTGATTGGGtt                                                          >  1:576600/1‑93 (MQ=255)
                                gcTTCGTAACCAAAAAAGCTTTTGCTTACGGCCTGAAGCCGATTGTTGTTATCAACAAGGTTGACCGCCCTGGCGCGCGTCCTGATTGGGTtg                                                         >  1:5120245/1‑93 (MQ=255)
                                 cTTCGTAACCAAAAAAGCGTTTGCTTACGGCCTGAAGCCGATTGTTGTTATCAACAAGGTTGACCGCCCTGGCGCGCGTCCTGATTGGGTtgt                                                        <  2:4422389/93‑1 (MQ=255)
                                 cTTCGTAACCAAAAAAGCGTTTGCTTACGGCCTGAAGCCGATTGTTGTTATCAACAAGGTTGACCGCCCTGGCGCGCGTCCTGATTGGGTtgt                                                        <  1:2184039/93‑1 (MQ=255)
                                  ttCGTAACCAAAAAAGCGTTTGCTTACGGCCTGAAGCCGATTGTTGTTATCAACAAGGTTGACCGCCCTGGCGCGCGTCCTGATTGGGTtgtg                                                       <  2:2687452/93‑1 (MQ=255)
                                  ttCGTAACCAAAAAAGCGTTTGCTTACGGCCTGAAGCCGATTGTTGTTATCAACAAGGTTGACCGCCCTGGCGCGCGTCCTGATTGGGTtgtg                                                       <  1:1671553/93‑1 (MQ=255)
                                   tCGTAACCAAAAAAGCGTTTGCTTACGGCCTGAAGCCGATTGTTGTTATCAACAAGGTTGACCGCCCTGGCGCGCGTCCTGATTGGGTTGTgg                                                      <  2:3964595/93‑1 (MQ=255)
                                    cGTAACCAAAAAAGCGTTTGCTTACGGCCTGAAGCCGATTGTTGTTATCAACAAGGTTGACCGCCCTGGCGCGCGTCCTGATTGGGTTGTGGa                                                     <  2:5827871/93‑1 (MQ=255)
                                    cGTAACCAAAAAAGCGTTTGCTTACGGCCTGAAGCCGATTGTTGTTATCAACAAGGTTGACCGCCCTGGCGCGCGTCCTGATTGGGTTGTGGa                                                     <  1:4269629/93‑1 (MQ=255)
                                      tAACCAAAAAAGCGTTTGCTTACGGCCTGAAGCCGATTGTTGTTATCAACAAGGTTGACCGCCCTGGCGCGCGTCCTGATTGGGTTGTGGATc                                                   >  1:1391493/1‑93 (MQ=255)
                                       aaCCAAAAAAGCGTTTGCTTACGGCCTGAAGCCGATTGTTGTTATCAACAAGGTTGACCGCCCTGGCGCGCGTCCTGATTGGGTTGTGGATCa                                                  <  2:3367811/93‑1 (MQ=255)
                                       aaCCAAAAAAGCGTTTGCTTACGGCCTGAAGCCGATTGTTGTTATCAACAAGGTTGACCGCCCTGGCGCGCGTCCTGATTGGGTTGTGGATCa                                                  <  1:5219351/93‑1 (MQ=255)
                                        aCCAAAAAAGCGTTTGCTTACGGCCTGAAGCCGATTGTTGTTATCAACAAGGTTGACCGCCCTGGCGCGCGTCCTGATTGGGTTGTGGATCAg                                                 >  2:4240085/1‑93 (MQ=255)
                                         ccAAAAAAGCGTTTGCTTACGGCCTGAAGCCGATTGTTGTTATCAACAAGGTTGACCGCCCTGGCGCGCGTCCTGATTGGGTTGTGGATCAgg                                                <  2:4373586/93‑1 (MQ=255)
                                          cAAAAAAGCGTTTGCTTACGGCCTGAAGCCGATTGTTGTTATCAACAAGGTTGACCGCCCTGGCGCGCGTCCTGATTGGGTTGTGGATCAGGt                                               <  1:1983568/93‑1 (MQ=255)
                                           aaaaaaGCGTTTGCTTACGGCCTGAAGCCGATTGTTGTTATCAACAAGGTTGACCGCCCTGGCGCGCGTCCTGATTGGGTTGTGGATCAGGTa                                              >  1:872577/1‑93 (MQ=255)
                                            aaaaaGCGTTTGCTTACGGCCTGAAGCCGATTGTTGTTATCAACAAGGTTGACCGCCCTGGCGCGCGTCCTGATTGGGTTGTGGATCAGGTAt                                             >  1:6052753/1‑93 (MQ=255)
                                            aaaaaGCGTTTGCTTACGGCCTGAAGCCGATTGTTGTTATCAACAAGGTTGACCGCCCTGGCGCGCGTCCTGATTGGGTTGTGGATCAGGTAt                                             >  1:3297884/1‑93 (MQ=255)
                                              aaaGCGTTTGCTTGCGGCCTGAAGCCGATTGTTGTTATCAACAAGGTTGACCGCCCTGGCGCGCGTCCTGATTGGGTTGTGGATCAGGTATTc                                           <  2:4037304/93‑1 (MQ=255)
                                              aaaGCGTTTGCTTACGGCCTGAAGCCGATTGTTGTTATCAACAAGGTTGACCGCCCTGGCGCGCGTCCTGATTGGGTTGTGGATCAGGTATTc                                           <  1:5101008/93‑1 (MQ=255)
                                                aGCGTTTGCTTACGGCCTGAAGCCGATTGTTGTTATCAACAAGGTTGACCGCCCTGGCGCGCGTCCTGATTGGGTTGTGGATCAGGTATTCGa                                         <  2:6144643/93‑1 (MQ=255)
                                                aGCGTTTGCTTACGGCCTGAAGCCGATTGTTGTTATCAACAAGGTTGACCGCCCTGGCGCGCGTCCTGATTGGGTTGTGGATCAGGTATTCGa                                         >  2:2453744/1‑93 (MQ=255)
                                                  cGTTTGCTTACGGCCTGAAGCCGATTGTTGTTATCAACAAGGTTGACCGCCCTGGCGCGCGTCCTGATTGGGTTGTGGATCAGGTATTCGATc                                       >  1:3977603/1‑93 (MQ=255)
                                                  cGTTTGCTTACGGCCTGAAGCCGATTGTTGTTATCAACAAGGTTGACCGCCCTGGCGCGCGTCCTGATTGGGTTGTGGATCAGGTATTCGATc                                       >  1:642721/1‑93 (MQ=255)
                                                    tttGCTTACGGCCTGAAGCCGATTGTTGTTATCAACAAGGTTGACCGCCCTGGCGCGCGTCCTGATTGGGTTGTGGATCAGGTATTCGATCTg                                     >  1:2505712/1‑93 (MQ=255)
                                                     ttGCTTACGGCCTGAAGCCGATTGTTGTTATCAACAAGGTTGACCGCCCTGGCGCGCGTCCTGATTGGGTTGTGGATCAGGTATTCGATCTGt                                    <  2:2271854/93‑1 (MQ=255)
                                                       gCTTACGGCCTGAAGCCGATTGTTGTTATCAACAAGGTTGACCGCCCTGGCGCGCGTCCTGATTGGGTTGTGGATCAGGTATTCGATCTGTTc                                  <  1:4521824/93‑1 (MQ=255)
                                                          tACGGCCTGAAGCCGATTGTTGTTATCAACAAGGTTGACCGCCCTGGCGCGCGTCCTGATTGGGTTGTGGATCAGGTATTCGATCTGTTCGtt                               >  1:5399240/1‑93 (MQ=255)
                                                           aCGGCCTGAAGCCGATTGTTGTTATCAACAAGGTTGACCGCCCTGGCGCGCGTCCTGATTGGGTTGTGGATCAGGTATTCGATCTGTTCGTTa                              >  2:6246184/1‑93 (MQ=255)
                                                              gCCTGAAGCCGATTGTTGTTATCAACAGGGTTGACCGCCCTGGCGCGCGTCCTGATTGGGTTGTGGATCAGGTATTCGATCTGTTCGTTAAcc                           <  2:5005877/93‑1 (MQ=255)
                                                              gCCTGAAGCCGATTGTTGTTATCAACAAGGTTGACCGCCCTGGCGCGCGTCCTGATTGGGTTGTGGATCAGGTATTCGATCTGTTCGTTAAcc                           <  1:1547000/93‑1 (MQ=255)
                                                              gCCTGAAGCCGATTGTTGTTATCAACAAGGTTGACCGCCCTGGCGCGCGTCCTGATTGGGTTGTGGATCAGGTATTCGATCTGTTCGTTAAcc                           <  1:3350456/93‑1 (MQ=255)
                                                               ccTGAAGCCGATTGTTGTTATCAACAAGGTTGACCGCCCTGGCGCGCGTCCTGATTGGGTTGTGGATCAGGTATTCGATCTGTTCGTTAACCt                          <  2:265141/93‑1 (MQ=255)
                                                                 tGAAGCCGATTGTTGTTATCAACAAGGTTGACCGCCCTGGCGCGCGTCCTGATTGGGTTGTGGATCAGGTATTCGATCTGTTCGTTAACCTCg                        >  1:77242/1‑93 (MQ=255)
                                                                 tGAAGCCGATTGTTGTTATCAACAAGGTTGACCGCCCTGGCGCGCGTCCTGATTGGGTTGTGGATCAGGTATTCGATCTGTTCGTTAACCTCg                        >  1:1898425/1‑93 (MQ=255)
                                                                   aaGCCGATTGTTGTTATCAACAAGGTTGACCGCCCTGGCGCGCGTCCTGATTGGGTTGTGGATCAGGTATTCGATCTGTTCGTTAACCTCGAc                      <  2:4512599/93‑1 (MQ=255)
                                                                    aGCCGATTGTTGTTATCAACAAGGTTGACCGCCCTGGCGCGCGTCCTGATTGGGTTGTGGATCAGGTATTCGATCTGTTCGTTAACCTCGAcg                     <  2:2009212/93‑1 (MQ=255)
                                                                     gCCGATTGTTGTTATCAACAAGGTTGACCGCCCTGGCGCGCGTCCTGATTGGGTTGTGGATCAGGTATTCGATCTGTTCGTTAACCTCGAcgc                    >  2:4693816/1‑93 (MQ=255)
                                                                        gATTGTTGTTATCAACAAGGTTGACCGCCCTGGCGCGCGTCCTGATTGGGTTGTGGATCAGGTATTCGATCTGTTCGTTAACCTCGACGcgac                 >  2:6120121/1‑93 (MQ=255)
                                                                        gATTGTTGTTATCAACAAGGTTGACCGCCCTGGCGCGCGTCCTGATTGGGTTGTGGATCAGGTATTCGATCTGTTCGTTAACCTCGACGcgac                 >  2:1664608/1‑93 (MQ=255)
                                                                         aTTGTTGTTATCAACAAGGTTGACCGCCCTGGCGCGCGTCCTGATTGGGTTGTGGATCAGTCATTCGATCTGTTCGTTAACCTCGACGcgacc                >  2:2157149/1‑93 (MQ=255)
                                                                         aTTGTTGTTATCAACAAGGTTGACCGCCCTGGCGCGCGTCCTGATTGGGTTGTGGATCAGGTATTCGATCTGTTCGTTAACCTCGACGcgacc                <  1:3883077/93‑1 (MQ=255)
                                                                          ttgttgTTATCAACAAGGTTGACCGCCCTGGCGCGCGTCCTGATTGGGTTGTGGATCAGGTATTCGATCTGTTCGTTAACCTCGACGcgaccg               >  2:3594388/1‑93 (MQ=255)
                                                                            gttgttATCAACAAGGTTGACCGCCCTGGCGCGCGTCCTGATTGGGTTGTGGATCAGGTATTCGATCTGTTCGTTAACCTCGACGcgaccgac             <  1:3627250/93‑1 (MQ=255)
                                                                             ttgttATCAACAAGGTTGACCGCCCTGGCGCGCGTCCTGATTGGGTTGTGGATCAGGTATTCGATCTGTTCGTTAACCTCGACGCGACcgacg            >  1:1949078/1‑93 (MQ=255)
                                                                             ttgttATCAACAAGGTTGACCGCCCTGGCGCGCGTCCTGATTGGGTTGTGGATCAGGTATTCGATCTGTTCGTTAACCTCGACGCGACcgacg            >  1:1704979/1‑93 (MQ=255)
                                                                              tgttATCAACAAGGTTGACCGCCCTGGCGCGCGTCCTGATTGGGTTGTGGATCAGGTATTCGATCTGTTCGTTAACCTCGACGCGACcgacga           <  2:1173344/93‑1 (MQ=255)
                                                                              tgttATCAACAAGGTTGACCGCCCTGGCGCGCGTCCTGATTGGGTTGTGGATCAGGTATTCGATCTGTTCGTTAACCTCGACGCGACcgacga           <  1:4062367/93‑1 (MQ=255)
                                                                              tgttATCAACAAGGTTGACCGCCCTGGCGCGCGTCCTGATTGGGTTGTGGATCAGGTATTCGATCTGTTCGTTAACCTCGACGCGACcgacga           <  1:2083985/93‑1 (MQ=255)
                                                                               gttATCAACAAGGTTGACCGCCCTGGCGCGCGTCCTGATTGGGTTGTGGATCAGGTATTCGATCTGTTCGTTAACCTCGACGCGACCGACGag          <  1:4054214/93‑1 (MQ=255)
                                                                                ttATCAACAAGGTTGACCGCCCTGGCGCGCGTCCTGATTGGGTTGTGGATCAGGTATTCGATCTGTTCGTTAACCTCGACGCGACCGACGaga         >  2:1555154/1‑92 (MQ=255)
                                                                                 tATCAACAAGGTTGACCGCCCTGGCGCGCGTCCTGATTGGGTTGTGGATCAGGTATTCGATCTGTTCGTTAACCTCGACGCGACCGACGagca        <  1:5911566/93‑1 (MQ=255)
                                                                                 tATCAACAAGGTTGACCGCCCTGGCGCGCGTCCTGATTGGGTTGTGGATCAGGTATTCGATCTGTTCGTTAACCTCGACGCGACCGACGagca        <  2:6219066/93‑1 (MQ=255)
                                                                                   tCAACAAGGTTGACCGCCCTGGCGCGCGTCCTGATTGGGTTGTGGATCAGGTATTCGATCTGTTCGTTAACCTCGACGCGACCGACGagcagc      >  2:5178622/1‑93 (MQ=255)
                                                                                   tCAACAAGGTTGACCGCCCTGGCGCGCGTCCTGATTGGGTTGTGGATCAGGTATTCGATCTGTTCGTTAACCTCGACGCGACCGACGagcagc      <  1:3144077/93‑1 (MQ=255)
                                                                                    caacgaGGTTGACCGCCCTGGCGCGCGTCCTGATTGGGTTGTGGATCAGGTATTCGATCTGTTCGTTAACCTCGACGCGACCGACGAGCAGCt     <  2:3853383/93‑1 (MQ=255)
                                                                                     aacaaGGTTGACCGCCCTGGCGCGCGTCCTGATTGGGTTGTGGATCAGGTATTCGATCTGTTCGTTAACCTCGACGCGACCGACGAGCAGCTg    <  1:2758455/93‑1 (MQ=255)
                                                                                     aacaaGGTTGACCGCCCTGGCGCGCGTCCTGATTGGGTTGTGGATCAGGTATTCGATCTGTTCGTTAACCTCGACGCGACCGACGAGCAGCTg    <  2:3775030/93‑1 (MQ=255)
                                                                                     aacaaGGTTGACCGCCCTGGCGCGCGTCCTGATTGGGTTGTGGATCAGGTATTCGATCTGTTCGTTAACCTCGACGCGACCGACGAGCAGCTg    >  1:3503345/1‑93 (MQ=255)
                                                                                     aacaaGGTTGACCGCCCTGGCGCGCGTCCTGATTGGGTTGTGGATCAGGTATTCGATCTGTTCGTTAACCTCGACGCGACCGACACGCCGCTg    >  2:3670766/1‑93 (MQ=255)
                                                                                      acaaGGTTGACCGCCCTGGCGCGCGTCCTGATTGGGTTGTGGATCAGGTATTCGATCTGTTCGTTAACCTCGACGCGACCGACGAGCAGCTgg   <  2:4085825/93‑1 (MQ=255)
                                                                                       caaGGTTGACCGCCCTGGCGCGCGTCCTGATTGGGTTGTGGATCAGGTATTCGATCTGTTCGTTAACCTCGACGCGACCGACGAGCAGCTGGa  <  1:3398226/93‑1 (MQ=255)
                                                                                          |                                                                                         
TGACGCATTTGACGGCCCGATGCCGCAAACGCGCTTCGTAACCAAAAAAGCGTTTGCTTACGGCCTGAAGCCGATTGTTGTTATCAACAAAGTTGACCGCCCTGGCGCGCGTCCTGATTGGGTTGTGGATCAGGTATTCGATCTGTTCGTTAACCTCGACGCGACCGACGAGCAGCTGGA  >  NC_000913/4056283‑4056462

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GGTTGACGCATTTGACGGCCCGATGCCGCAAACGCGCTTCGTAACCAAAAAAGCGTTTGCTTACGGCCTGAAGCCGATTGTTGTTATCAACAAAGTTGACCGCCCTGGCGCGCGTCCTGATTGGGTTGTGGATCAGGTATTCGATCTGTTCGTTAACCTCGACGCGACCGACGAGCAGCTGGACTTC  >  NC_000913/4056280‑4056466
                                                                                             |                                                                                             
GGTTGACGCATTTGACGGCCCGATGCCGCAAACGCGCTTCGTAACCAAAAAAGCGTTTGCTTACGGCCTGAAGCCGATTGTTGTTATCAACAAG                                                                                               <  SRR4302533.940690/94‑1 (MQ=60)
  TTGACGCATTTGACGGCCCGATGCCGCAAACGCGCTTCGTAACCAAAAAAGCGTTTGCTTACGGCCTGAAGCCGATTGTTGTTATCAACAAGGT                                                                                             >  SRR4302533.2141148/1‑94 (MQ=60)
  TTGACGCATTTGACGGCCCGATGCCGCAAACGCGCTTCGTAACCAAAAAAGCGTTTGCTTACGGCCTGAAGCCGATTGTTGTTATCAACAAGGT                                                                                             >  SRR4302533.4818205/1‑94 (MQ=60)
   TGACGCATTTGACGGCCCGATGCCGCAAACGCGCTTCGTAACCAAAAAAGCGTTTGCTTACGGCCTGAAGCCGATTGTTGTTATCAACAAGGTT                                                                                            >  SRR4302533.5140134/1‑94 (MQ=60)
   TGACGCATTTGACGGCCCGATGCCGCAAACGCGCTTCGTAACCAAAAAAGCGTTTGCTTACGGCCTGAAGCCGATTGTTGTTATCAACAAGGTT                                                                                            >  SRR4302533.3723940/1‑94 (MQ=60)
    GACGCATTTGACGGCCCGATGCCGCAAACGCGCTTCGTAACCAAAAAAGCGTTTGCTTACGGCCTGAAGCCGATTGTTGTTATCAACAAGGTTG                                                                                           <  SRR4302533.5772166/94‑1 (MQ=60)
    GACGCATTTGACGGCCCGATGCCGCAAACGCGCTTCGTAACCAAAAAAGCGTTTGCTTACGGCCTGAAGCCGATTGTTGTTATCAACAAGGTTG                                                                                           <  SRR4302533.2971923/94‑1 (MQ=60)
     ACACATTTGACGGCCCGATGCCGCAAACGCGCTTCGTAACCAAAAAAGCGTTTGCTTACGGCCTGACGCCGATTGTTGTTATCAACAAGGTTGA                                                                                          <  SRR4302533.2188558/94‑1 (MQ=60)
      CGCATTTGACGGCCCGATGCCGCAAACGCGCTTCGTAACCAAAAAAGCGTTTGCTTACGGCCTGAAGCCGATTGTTGTTATCAACAAGGTTGAC                                                                                         <  SRR4302533.3832418/94‑1 (MQ=60)
       GCATTTGACGGCCCGATGCCGCAAACGCGCTTCGTAACCAAAAAAGCGTTTGCTTACGGCCTGAAGCCGATTGTTGTTATCAACAAGGTTGACC                                                                                        >  SRR4302533.4003704/1‑94 (MQ=60)
       GCATTTGACGGCCCGATGCCGCAAACGCGCTTCGTAACCAAAAAAGCGTTTGCTTACGGCCTGAAGCCGATTGTTGTTATCAACAAGGTTGACC                                                                                        <  SRR4302533.4763645/94‑1 (MQ=60)
       GCATTTGACGGCCCGATGCCGCAAACGCGCTTCGTAACCAAAAAAGCGTTTGCTTACGGCCTGAAGCCGATTGTTGTTATCAACAAGGTTGACC                                                                                        >  SRR4302533.2335146/1‑94 (MQ=60)
       GCATTTGACGGCCCGATGCCGCAAACGCGCTTCGTAACCAAAAAAGCGTTTGCTTACGGCCTGAAGCCGATTGTTGTTATCAACAAGGTTGACC                                                                                        <  SRR4302533.1221742/94‑1 (MQ=60)
       GCATTTGACGGCCCGATGCCGCAAACGCGCTTCGTAACCAAAAAAGCGTTTGCTTACGGCCTGAAGCCGATTGTTGTTATCAACAAGGTTGACC                                                                                        <  SRR4302533.3491612/94‑1 (MQ=60)
        CATTTGACGGCCCGATGCCGCAAACGCGCTTCGTAACCAAAAAAGCGTTTGCTTACGGCCTGAAGCCGATTGTTGTTATCAACAAGGTTGACCG                                                                                       >  SRR4302533.2772590/1‑94 (MQ=60)
        CATTTGACGGCCCGATGCCGCAAACGCGCTTCGTAACCAAAAAAGCGTTTGCTTACGGCCTGAAGCCGATTGTTGTTATCAACAAGGTTGACCG                                                                                       <  SRR4302533.2115827/94‑1 (MQ=60)
        CATTTGACGGCCCGATGCCGCAAACGCGCTTCGTAACCAAAAAAGCGTTTGCTTACGGCCTGAAGCCGATTGTTGTTATCAACAAGGTTGACCG                                                                                       <  SRR4302533.5176721/94‑1 (MQ=60)
        CATTTGACGGCCCGATGCCGCAAACGCGCTTCGTAACCAAAAAAGCGTTTGCTTACGGCCTGAAGCCGATTGTTGTTATCAACAAGGTTGACCG                                                                                       >  SRR4302533.6174171/1‑94 (MQ=60)
         ATTTGACGGCCCGATGCCGCAAACGCGCTTCGTAACCAAAAAAGCGTTTGCTTACGGCCTGAAGCCGATTGTTGTTATCAACAAGGTTGACCGC                                                                                      >  SRR4302533.1201094/1‑94 (MQ=60)
         ATTTGACGGCCCGATGCCGCAAACGCGCTTCGTAACCAAAAAAGCGTTTGCTTACGGCCTGAAGCCGATTGTTGTTATCAACAAGGTTGACCGC                                                                                      >  SRR4302533.1690244/1‑94 (MQ=60)
         ATTTGACGGCCCGATGCCGCAAACGCGCTTCGTAACCAAAAAAGCGTTTGCTTACGGCCTGAAGCCGATTGTTGTTATAAACAAGGTTGACCGC                                                                                      >  SRR4302533.4305729/1‑94 (MQ=60)
             GACGGCCCGATGCCGCAAACGCGCTTCGTAACCAAAAAAGCGTTTGCTTACGGCCTGAAGCCGATTGTTGTTATCAACAAGGTTGACCGCCCTG                                                                                  <  SRR4302533.68764/94‑1 (MQ=60)
             GACGGCCCGATGCCGCAAACGCGCTTCGTAACCAAAAAAGCGTTTGCTTACGGCCTGAAGCCGATTGTTGTTATCAACAAGGTTGACCGCCCTG                                                                                  <  SRR4302533.2580405/94‑1 (MQ=60)
             GACGGCCCGATGCCGCAAACGCGCTTCGTAACCAAAAAAGCGTTTGCTTACGGCCTGAAGCCGATTGTTGTTATCAACAAGGTTGACCGCCCTG                                                                                  <  SRR4302533.5310202/94‑1 (MQ=60)
              ACGGCCCGATGCCGCAAACGCGCTTCGTAACCAAAAAAGCGTTTGCTTACGGCCTGAAGCCGATTGTTGTTATCAACAAGGTTGACCGCCCTGG                                                                                 >  SRR4302533.2118409/1‑94 (MQ=60)
               CGGCCCGATGCCGCAAACGCGCTTCGTAACCAAAAAAGCGTTTGCTTACGGCCTGAAGCCGATTGTTGTTATCAACAAGGTTGACCGCCCTGGC                                                                                <  SRR4302533.2766447/94‑1 (MQ=60)
               CGGCCCGATGCCGCAAACGCGCTTCGTAACCAAAAAAGCGTTTGCTTACGGCCTGAAGCCGATTGTTGTTATCAACAAGGTTGACCGCCCTGGC                                                                                >  SRR4302533.5746839/1‑94 (MQ=60)
                GGCCCGATGCCGCAAACGCGCTTCGTAACCAAAAAAGCGTTTGCTTACGGCCTGAAGCCGATTGTTGTTATCAACAAGGTTGACCGCCCTGGCG                                                                               >  SRR4302533.4244836/1‑94 (MQ=60)
                GGCCCGATGCCGCAAACGCGCTTCGTAACCAAAAAAGCGTTTGCTTACGGCCTGAAGCCGATTGTTGTTATCAACAAGGTTGACCGCCCTGGCG                                                                               <  SRR4302533.5870362/94‑1 (MQ=60)
                GGCCCGATGCCGCAAACGCGCTTCGTAACCAAAAAAGCGTTTGCTTACGGCCTGAAGCCGATTGTTGTTATCAACAAGGTTGACCGCCCTGGCG                                                                               <  SRR4302533.5824778/94‑1 (MQ=60)
                  CCCGATGCCGCAAACGCGCTTCGTAACCAAAAAAGCGTTTGCTTACGGCCTGAAGCCGATTGTTGTTATCAACAAGGTTGACCGCCCTGGCGCG                                                                             <  SRR4302533.4920425/94‑1 (MQ=60)
                   CCGATGCCGCAAACGCGCTTCGTAACCAAAAAAGCGTTTGCTTACGGCCTGAAGCCGATTGTTGTTATCAACAAGGTTGACCGCCCTGGCGCGC                                                                            >  SRR4302533.5754451/1‑94 (MQ=60)
                    CGATGCCGCAAACGCGCTTCGTAACCAAAAAAGCGTTTGCTTACGGCCTGAAGCCGATTGTTGTTATCAACAAGGTTGACCGCCCTGGCGCGCG                                                                           >  SRR4302533.3277832/1‑94 (MQ=60)
                    CGATGCCGCAAACGCGCTTCGTAACCAAAAAAGCGTTTGCTTACGGCCTGAAGCCGATTGTTGTTATCAACAAGGTTGACCGCCCTGGCGCGCG                                                                           >  SRR4302533.1197721/1‑94 (MQ=60)
                       TGCCGCAAACGCGCTTCGTAACCAAAAAAGCGTTTGCTTACGGCCTGAAGCCGATTGTTGTTATCAACAAGGTTGACCGCCCTGGCGCGCGTCC                                                                        <  SRR4302533.5697290/94‑1 (MQ=60)
                       TGCCGCAAACGCGCTTCGTAACCAAAAAAGCGTTTGCTTACGGCCTGAAGCCGATTGTTGTTATCAACAAGGTTGACCGCCCTGGCGCGCGTCC                                                                        <  SRR4302533.904110/94‑1 (MQ=60)
                       TGCCGCAAACGCGCTTCGTAACCAAAAAAGCGTTTGCTTACGGCCTGAAGCCGATTGTTGTTATCAACAAGGTTGACCGCCCTGGCGCGCGTCC                                                                        <  SRR4302533.6029408/94‑1 (MQ=60)
                       TGCCGCAAACGCGCTTCGTAACCAAAAAAGCGTTTGCTTACGGCCTGAAGCCGATTGTTGTTATCAACAAGGATGACCGCCCTGGCGCGCGTCC                                                                        >  SRR4302533.150628/1‑94 (MQ=60)
                        GCCGCAAACGCGCTTCGTAACCAAAAAAGCGTTTGCTTACGGCCTGAAGCCGATTGTTGTTATCAACAAGGTTGACCGCCCTGGCGCGCGTCCT                                                                       <  SRR4302533.3614121/94‑1 (MQ=60)
                         CCGCAAACGCGCTTCGTAACCAAAAAAGCGTTTGCTTACGGCCTGAAGCCGATTGTTGTTATCAACAAGGTTGACCGCCCTGGCGCGCGTCCTG                                                                      <  SRR4302533.1826223/94‑1 (MQ=60)
                         CCGCAAACGCGCTTCGTAACCAAAAAAGCGTTTGCTTACGGCCTGAAGCCGATTGTTGTTATCAACAAGGTTGACCGCCCTGGCGCGCGTCCTG                                                                      <  SRR4302533.5548182/94‑1 (MQ=60)
                         CCGCAAACGCGCTTCGTAACCAAAAAAGCGTTTGCTTACGGCCTGAAGCCGATTGTTGTTATCAACAAGGTTGACCGCCCTGGCGCGCGTCCTG                                                                      <  SRR4302533.923445/94‑1 (MQ=60)
                         CCGCAAACGCGCTTCGTAACCAAAAAAGCGTTTGCTTACGGCCTGAAGCCGATTGTTGTTATCAACAAGGTTGACCGCCCTGGCGCGCGTCCTG                                                                      <  SRR4302533.1629969/94‑1 (MQ=60)
                         CCGCAAACGCGCTTCGTAACCAAAAAAGCGTTTGCTTACGGCCTGAAGCCGATTGTTGTTATCAACAAGGTTGACCGCCCTGGCGCGCGTCCTG                                                                      >  SRR4302533.4451824/1‑94 (MQ=60)
                          CGCAAACGCGCTTCGTAACCAAAAAAGCGTTTGCTTACGGCCTGAAGCCGATTGTTGTTATCAACAAGGTTGACCGCCCTGGCGCGCGTCCTGA                                                                     <  SRR4302533.4872517/94‑1 (MQ=60)
                           GCAAACGCGCTTCGTAACCAAAAAAGCGTTTGCTTACGGCCTGAAGCCGATTGTTGTTATCAACAAGGTTGACCGCCCTGGCGCGCGTCCTGAT                                                                    >  SRR4302533.797326/1‑94 (MQ=60)
                            CAAACGCGCTTCGTAACCAAAAAAGCGTTTGCTTACGGCCTGAAGCCGATTGTTGTTATCAACAAGGTTGACCGCCCTGGCGCGCGTCCTGATT                                                                   >  SRR4302533.1297456/1‑94 (MQ=60)
                             AAGCGCGCTTCGTAACCAAAAAAGCGTTTGCTTACGGCCTGAAGCCGATTGTTGTTATCAACAAGGTTGACCGCCCTGGCGCGCGTCCTGATTG                                                                  <  SRR4302533.2334339/94‑1 (MQ=60)
                             AAACGCGCTTCGTAACCAAAAAAGCGTTTGCTTACGGCCTGAAGCCGATTGTTGTTATCAACAAGGTTGACCGCCCTGGCGCGCGTCCTGATTG                                                                  >  SRR4302533.684347/1‑94 (MQ=60)
                              AACGCGCTTCGTAACCAAAAAAGCGTTTGCTTACGGCCTGAAGCCGATTGTTGTTATCAACAAGGTTGACCGCCCTGGCGCGCGTCCTGATTGG                                                                 >  SRR4302533.4862761/1‑94 (MQ=60)
                               ACGCGCTTCGTAACCAAAAAAGCGTTTGCTTACGGCCTGAAGCCGATTGTTGTTATCAACAAGGTTGACCGCCCTGGCGCGCGTCCTGATTGGG                                                                >  SRR4302533.1133463/1‑94 (MQ=60)
                                CGCGCTTCGTAACCAAAAAAGCGTTTGCTTACGGCCTGAAGCCGATTGTTGTTATCAACAAGGTTGACCGCCCTGGCGCGCGTCCTGATTGGGT                                                               >  SRR4302533.1109324/1‑94 (MQ=60)
                                CGCGCTTCGTAACCAAAAAAGCGTTTGCTTACGGCCTGAAGCCGATTGTTGTTATCAACAAGGTTGACCGCCCTGGCGCGCGTCCTGATTGGGT                                                               <  SRR4302533.4084507/94‑1 (MQ=60)
                                 GCGCTTCGTAACCAAAAAAGCGTTTGCTTACGGCCTGAAGCCGATTGTTGTTATCAACAAGGTTGACCGCCCTGGCGCGCGTCCTGATTGGGTT                                                              >  SRR4302533.580001/1‑94 (MQ=60)
                                 GCGCTTCGTAACCAAAAAAGCGTTTGCTTACGGCCTGAAGCCGATTGTTGTTATCAACAAGGTTGACCGCCCTGGCGCGCGTCCTGATTGGGTT                                                              <  SRR4302533.5770707/94‑1 (MQ=60)
                                 GCGCTTCGTAACCAAAAAAGCGTTTGCTTACGGCCTGAAGCCGATTGTTGTTATCAACAAGGTTGACCGCCCTGGCGCGCGTCCTGATTGGGTT                                                              <  SRR4302533.3389762/94‑1 (MQ=60)
                                 GCGCTTCGTAACCAAAAAAGCGTTTGCTTACGGCCTGAAGCCGATTGTTGTTATCAACAAGGTTGACCGCCCTGGCGCGCGTCCTGATTGGGTT                                                              >  SRR4302533.5120276/1‑94 (MQ=60)
                                  CGCTTCGTAACCAAAAAAGCTTTTGCTTACGGCCTGAAGCCGATTGTTGTTATCAACAAGGTTGACCGCCCTGGCGCGCGTCCTGATTGGGTTG                                                             >  SRR4302533.5151179/1‑94 (MQ=60)
                                  CGCTTCGTAACCAAAAAAGCGTTTGCTTACGGCCTGAAGCCGATTGTTGTTATCAACAAGGTTGACCGCCCTGGCGCGCGTCCTGATTGGGTTG                                                             >  SRR4302533.6305915/1‑94 (MQ=60)
                                  CGCTTCGTAACCAAAAAAGCGTTTGCTTACGGCCTGAAGCCGATTGTTGTTATCAACAAGGTTGACCGCCCTGGCGCGCGTCCTGATTGGGTTG                                                             >  SRR4302533.2505281/1‑94 (MQ=60)
                                   GCTTCGTAACCAAAAAAGCGTTTGCTTACGGCCTGAAGCCGATTGTTGTTATCGACAAGGTTGACCGCCCTGGCGCGCGTCCTGATTGGGTTGT                                                            >  SRR4302533.3992021/1‑94 (MQ=60)
                                    CTTCGTAACCAAAAAAGCGTTTGCTTACGGCCTGAAGCCGATTGTTGTTATCAACAAGGTTGACCGCCCTGGCGCGCGTCCTGATTGGGTTGTG                                                           <  SRR4302533.2197503/94‑1 (MQ=60)
                                    CTTCGTAACCAAAAAAGCGTTTGCTTACGGCCTGAAGCCGATTGTTGTTATCAACAAGGTTGACCGCCCTGGCGCGCGTCCTGATTGGGTTGTG                                                           >  SRR4302533.294378/1‑94 (MQ=60)
                                     TTCGTAACCAAAAAAGCGTTTGCTTACGGCCTGAAGCCGATTGTTGTTATCAACAAGGTTGACCGCCCTGGCGCGCGTCCTGATTGGGTTGTGG                                                          <  SRR4302533.3295367/94‑1 (MQ=60)
                                     TTCGTAACCAAAAAAGCGTTTGCTTACGGCCTGAAGCCGATTGTTGTTATCAACAAGGTTGACCGCCCTGGCGCGCGTCCTGATTGGGTTGTGG                                                          <  SRR4302533.1681656/94‑1 (MQ=60)
                                      TCGTAACCAAAAAAGCGTTTGCTTACGGCCTGAAGCCGATTGTTGTTATCAACAAGGTTGACCGCCCTGGCGCGCGTCCTGATTGGGTTGTGGA                                                         >  SRR4302533.384661/1‑94 (MQ=60)
                                      TCGTAACCAAAAAAGCGTTTGCTTACGGCCTGAAGCCGATTGTTGTTATCAACAAGGTTGACCGCCCTGGCGCGCGTCCTGATTGGGTTGTGGA                                                         <  SRR4302533.4475165/94‑1 (MQ=60)
                                      TCGTAACCAAAAAAGCGTTTGCTTACGGCCTGAAGCCGATTGTTGTTATCAACAAGGTTGACCGCCCTGGCGCGCGTCCTGATTGGGTTGTGGA                                                         <  SRR4302533.5559673/94‑1 (MQ=60)
                                       CGTAACCAAAAAAGCGTTTGCTTACGGCCTGAAGCCGATTGTTGTTATCAACAAGGTTGACCGCCCTGGCGCGCGTCCTGATTGGGTTGTGGAT                                                        >  SRR4302533.3726531/1‑94 (MQ=60)
                                       CGTAACCAAAAAAGCGTTTGCTTACGGCCTGAAGCCGATTGTTGTTATCAACAAGGTTGACCGCCCTGGCGCGCGTCCTGATTGGGTTGTGGAT                                                        <  SRR4302533.1556465/94‑1 (MQ=60)
                                       CGTAACCAAAAAAGCGTTTGCTTACGGCCTGAAGCCGATTGTTGTTATCAACAAGGTTGACCGCCCTGGCGCGCGTCCTGATTGGGTTGTGGAT                                                        <  SRR4302533.4295331/94‑1 (MQ=60)
                                        GTAACCAAAAAAGCGTTTGCTTACGGCCTGAAGCCGATTGTTGTTATCAACAAGGTTGACCGCCCTGGCGCGCGTCCTGATTGGGTTGTGGATC                                                       >  SRR4302533.1399875/1‑94 (MQ=60)
                                        GTAACCAAAAAAGCGTTTGCTTACGGCCTGAAGCCGATTGTTGTTATCAACAAGGTTGACCGCCCTGGCGCGCGTCCTGATTGGGTTGTGGATC                                                       >  SRR4302533.3245577/1‑94 (MQ=60)
                                          AACCAAAAAAGCGTTTGCTTACGGCCTGAAGCCGATTGTTGTTATCAACAAGGTTGACCGCCCTGGCGCGCGTCCTGATTGGGTTGTGGATCAG                                                     <  SRR4302533.5250764/94‑1 (MQ=60)
                                           ACCAAAAAAGCGTTTGCTTACGGCCTGAAGCCGATTGTTGTTATCAACAAGGTTGACCGCCCTGGCGCGCGTCCTGATTGGGTTGTGGATCAGG                                                    >  SRR4302533.3419125/1‑94 (MQ=60)
                                            CCAAAAAAGCGTTTGCTTACGGCCTGAAGCCGATTGTTGTTATCAACAAGGTTGACCGCCCTGGCGCGCGTCCTGATTGGGTTGTGGATCAGGT                                                   >  SRR4302533.2023178/1‑94 (MQ=60)
                                             CAAAAAAGCGTTTGCTTACGGCCTGAAGCCGATTGTTGTTATCAACAAGGTTGACCGCCCTGGCGCGCGTCCTGATTGGGTTGTGGATCAGGTA                                                  >  SRR4302533.877699/1‑94 (MQ=60)
                                             CAAAAAAGCGTTTGCTTACGGCCTGAAGCCGATTGTTGTTATCAACAAGGTTGACCGCCCTGGCGCGCGTCCTGATTGGGTTGTGGATCAGGTA                                                  <  SRR4302533.1995706/94‑1 (MQ=60)
                                              AAAAAAGCGTTTGCTTACGGCCTGAAGCCGATTGTTGTTATCAACAAGGTTGACCGCCCTGGCGCGCGTCCTGATTGGGTTGTGGATCAGGTAT                                                 <  SRR4302533.3553439/94‑1 (MQ=60)
                                              AAAAAAGCGTTTGCTTACGGCCTGAAGCCGATTGTTGTTATCAACAAGGTTGACCGCCCTGGCGCGCGTCCTGATTGGGTTGTGGATCAGGTAT                                                 >  SRR4302533.6089412/1‑94 (MQ=60)
                                              AAAAAAGCGTTTGCTTACGGCCTGAAGCCGATTGTTGTTATCAACAAGGTTGACCGCCCTGGCGCGCGTCCTGATTGGGTTGTGGATCAGGTAT                                                 >  SRR4302533.3318237/1‑94 (MQ=60)
                                               AAAAAGCGTTTGCTTACGGCCTGAAGCCGATTGTTGTTATCAACAAGGTTGACCGCCCTGGCGCGCGTCCTGATTGGGTTGTGGATCAGGTATT                                                <  SRR4302533.1312882/94‑1 (MQ=60)
                                                 AAAGCGTTTGCTTACGGCCTGAAGCCGATTGTTGTTATCAACAAGGTTGACCGCCCTGGCGCGCGTCCTGATTGGGTTGTGGATCAGGTATTCG                                              <  SRR4302533.5131821/94‑1 (MQ=60)
                                                    GCGTTTGCTTACGGCCTGAAGCCGATTGTTGTTATCAACAAGGTTGACCGCCCTGGCGCGCGTCCTGATTGGGTTGTGGATCAGGTATTCGATC                                           >  SRR4302533.4001820/1‑94 (MQ=60)
                                                    GCGTTTGCTTACGGCCTGAAGCCGATTGTTGTTATCAACAAGGTTGACCGCCCTGGCGCGCGTCCTGATTGGGTTGTGGATCAGGTATTCGATC                                           >  SRR4302533.646498/1‑94 (MQ=60)
                                                      GTTTGCTTACGGCCTGAAGCCGATTGTTGTTATCAACAAGGTTGACCGCCCTGGCGCGCGTCCTGATTGGGTTGTGGATCAGGTATTCGATCTG                                         >  SRR4302533.5476726/1‑94 (MQ=60)
                                                      GTTTGCTTACGGCCTGAAGCCGATTGTTGTTATCAACAAGGTTGACCGCCCTGGCGCGCGTCCTGATTGGGTTGTGGATCAGGTATTCGATCTG                                         <  SRR4302533.4404157/94‑1 (MQ=60)
                                                      GTTTGCTTACGGCCTGAAGCCGATTGTTGTTATCAACAAGGTTGACCGCCCTGGCGCGCGTCCTGATTGGGTTGTGGATCAGGTATTCGATCTG                                         >  SRR4302533.2521160/1‑94 (MQ=60)
                                                        TTGCTTACGGCCTGAAGCCGATTGTTGTTATCAACAAGGTTGACCGCCCTGGCGCGCGTCCTGATTGGGTTGTGGATCAGGTATTCGATCTGTT                                       >  SRR4302533.1943355/1‑94 (MQ=60)
                                                         TGCTTACGGCCTGAAGCCGATTGTTGTTATCAACAAGGTTGACCGCCCTGGCGCGCGTCCTGATTGGGTTGTGGATCAGGTATTCGATCTGTTC                                      <  SRR4302533.3317386/94‑1 (MQ=60)
                                                          TCTTACGGCCTGAAGCCGATTGTTGTTATCAACAAGGTTGACCGCCCTGGCGCGCGTCCTGATTGGGTTGTGGATCAGGTATTCGATCTGTTCG                                     <  SRR4302533.1978631/94‑1 (MQ=60)
                                                          GCTTACGGCCTGAAGCCGATTGTTGTTATCAACAAGGTTGACCGCCCTGGCGCGCGTCCTGATTGGGTTGTGGATCAGGTATTCGATCTGTTCG                                     <  SRR4302533.6202569/94‑1 (MQ=60)
                                                          GCTTACGGCCTGAAGCCGATTGTTGTTATCAACAAGGTTGACCGCCCTGGCGCGCGTCCTGATTGGGTTGTGGATCAGGTATTCGATCTGTTCG                                     <  SRR4302533.4549202/94‑1 (MQ=60)
                                                           CTTACGGCCTGAAGCCGATTGTTGTTATCAACAAGGTTGACCGCCCTGGCGCGCGTCCTGATTGGGTTGTGGATCAGGTATTCGATCTGTTCGT                                    >  SRR4302533.4880844/1‑94 (MQ=60)
                                                           CTTACGGCCTGAAGCCGATTGTTGTTATCAACAAGGTTGACCGCCCTGGCGCGCGTCCTGATTGGGTTGTGGATCAGGTATTCGATCTGTTCGT                                    <  SRR4302533.4704363/94‑1 (MQ=60)
                                                           CTTACGGCCTGAAGCCGATTGTTGTTATCAACAAGGTTGACCGCCCTGGCGCGCGTCCTGATTGGGTTGTGGATCAGGTATTCGATCTGTTCGT                                    <  SRR4302533.337052/94‑1 (MQ=60)
                                                           CTTACGGCCTGAAGCCGATTGTTGTTATCAACAAGGTTGACCGCCCTGGCGCGCGTCCTGATTGGGTTGTGGATCAGGCATTCGATCTGTTCGT                                    >  SRR4302533.2010020/1‑94 (MQ=60)
                                                            TTACGGCCTGAAGCCGATTGTTGTTATCAACAAGGTTGACCGCCCTGGCGCGCGTCCTGATTGGGTTGTGGATCAGGTATTCGATCTGTTCGTT                                   <  SRR4302533.4032820/94‑1 (MQ=60)
                                                            TTACGGCCTGAAGCCGATTGTTGTTATCAACAAGGTTGACCGCCCTGGCGCGCGTCCTGATTGGGTTGTGGATCAGGTATTCGATCTGTTCGTT                                   >  SRR4302533.3947134/1‑94 (MQ=60)
                                                            TTACGGCCTGAAGCCGATTGTTGTTATCAACAAGGTTGACCGCCCTGGCGCGCGTCCTGATTGGGTTGTGGATCAGGTATTCGATCTGTTCGTT                                   >  SRR4302533.5431686/1‑94 (MQ=60)
                                                              ACGGCCTGAAGCCGATTGTTGTTATCAACAAGGTTGACCGCCCTGGCGCGCGTCCTGATTGGGTTGTGGATCAGGTATTCGATCTGTTCGTTAA                                 >  SRR4302533.6046728/1‑94 (MQ=60)
                                                               CGGCCTGAAGCCGATTGTTGTTATCAACAAGGTTGACCGCCCTGGCGCGCGTCCTGATTGGGTTGTGGATCAGGTATTCGATCTGTTCGTTAAC                                <  SRR4302533.1837844/94‑1 (MQ=60)
                                                                 GCCTGAAGCCGATTGTTGTTATCAACAAGGTTGACCGCCCTGGCGCGCGTCCTGATTGGGTTGTGGATCAGGTATTCGATCTGTTCGTTAACCT                              >  SRR4302533.2275020/1‑94 (MQ=60)
                                                                 GCCTGAAGCCGATTGTTGTTATCAACAAGGTTGACCGCCCTGGCGCGCGTCCTGATTGGGTTGTGGATCAGGTATTCGATCTGTTCGTTAACCT                              <  SRR4302533.1556323/94‑1 (MQ=60)
                                                                 GCCTGAAGCCGATTGTTGTTATCAACAAGGTTGACCGCCCTGGCGCGCGTCCTGATTGGGTTGTGGATCAGGTATTCGATCTGTTCGTTAACCT                              <  SRR4302533.3371154/94‑1 (MQ=60)
                                                                   CTGAAGCCGATTGTTGTTATCAACAAGGTTGACCGCCCTGGCGCGCGTCCTGATTGGGTTGTGGATCAGGTATTCGATCTGTTCGTTAACCTCG                            >  SRR4302533.1910016/1‑94 (MQ=60)
                                                                   CTGAAGCCGATTGTTGTTATCAACAAGGTTGACCGCCCTGGCGCGCGTCCTGATTGGGTTGTGGATCAGGTATTCGATCTGTTCGTTAACCTCG                            >  SRR4302533.77740/1‑94 (MQ=60)
                                                                   CTGAAGCCGATTGTTGTTATCAACAAGGTTGACCGCCCTGGCGCGCGTCCTGATTGGGTTGTGGATCAGGTATTCGATCTGTTCGTTAACCTCG                            >  SRR4302533.5139997/1‑94 (MQ=60)
                                                                    TGAAGCCGATTGTTGTTATCAACAAGGTTGACCGCCCTGGCGCGCGTCCTGATTGGGTTGTGGATCAGGTATTCGATCTGTTCGTTAACCTCGA                           >  SRR4302533.6109213/1‑94 (MQ=60)
                                                                     GAAGCCGATTGTTGTTATCAACAAGGTTGACCGCCCTGGCGCGCGTCCTGATTGGGTTGTGGATCAGGTATTCGATCTGTTCGTTAACCTCGAC                          <  SRR4302533.1500288/94‑1 (MQ=60)
                                                                     GAAGCCGATTGTTGTTATCAACAAGGTTGACCGCCCTGGCGCGCGTCCTGATTGGGTTGTGGATCAGGTATTCGATCTGTTCGTTAACCTCGAC                          >  SRR4302533.1952757/1‑94 (MQ=60)
                                                                     GAAGCCGATTGTTGTTATCAACAAGGTTGACCGCCCTGGCGCGCGTCCTGATTGGGTTGTGGATCAGGTATTCGATCTGTTCGTTAACCTCGAC                          <  SRR4302533.4921587/94‑1 (MQ=60)
                                                                      AAGCCGATTGTTGTTATCAACAAGGTTGACCGCCCTGGCGCGCGTCCTGATTGGGTTGTGGATCAGGTATTCGATCTGTTCGTTAACCTCGACG                         <  SRR4302533.4847140/94‑1 (MQ=60)
                                                                        GCCGATTGTTGTTATCAACAAGGTTGACCGCCCTGGCGCGCGTCCTGATTGGGTTGTGGATCAGGTATTCGATCTGTTCGTTAACCTCGACGCG                       <  SRR4302533.3190867/94‑1 (MQ=60)
                                                                        GCCGATTGTTGTTATCAACAAGGTTGACCGCCCTGGCGCGCGTCCTGATTGGGTTGTGGATCAGGTATTCGATCTGTTCGTTAACCTCGACGCG                       <  SRR4302533.2565271/94‑1 (MQ=60)
                                                                          CGATTGTTGTTATCAACAAGGTTGACCGCCCTGGCGCGCGTCCTGATTGGGTTGTGGATCAGGTATTCGATCTGTTCGTTAACCTCGACGCGAC                     >  SRR4302533.2417269/1‑94 (MQ=60)
                                                                           GATTGTTGTTATCAACAAGGTTGACCGCCCTGGCGCGCGTCCTGATTGGGTTGTGGATCAGGTATTCGATCTGTTCGTTAACCTCGACGCGACC                    >  SRR4302533.5514664/1‑94 (MQ=60)
                                                                            ATTGTTGTTATCAACAAGGTTGACCGCCCTGGCGCGCGTCCTGATTGGGTTGTGGATCAGGTATTCGATCTGTTCGTTAACCTCGACGCGACCG                   <  SRR4302533.3906772/94‑1 (MQ=60)
                                                                              TGTTGTTATCAACAAGGTTGACCGCCCTGGCGCGCGTCCTGATTGGGTTGTGGATCAGGTATTCGATCTGTTCGTTAACCTCGACGCGACCGAC                 <  SRR4302533.920804/94‑1 (MQ=60)
                                                                               GTTGTTATCAACAAGGTTGACCGCCCTGGCGCGCGTCCTGATTGGGTTGTGGATCAGGTATTCGATCTGTTCGTTAACCTCGACGCGACCGACG                <  SRR4302533.3649570/94‑1 (MQ=60)
                                                                               GTTGTTATCAACAAGGTTGACCGCCCTGGCGCGCGTCCTGATTGGGTTGTGGATCAGGTATTCGATCTGTTCGTTAACCTCGACGCGACCGACG                <  SRR4302533.2959603/94‑1 (MQ=60)
                                                                               GTTGTTATCAACAAGGTTGACCGCCCTGGCGCGCGTCCTGATTGGGTTGTGGATCAGGTATTCGATCTGTTCGTTAACCTCGACGCGACCGACG                >  SRR4302533.1715305/1‑94 (MQ=60)
                                                                               GTTGTTATCAACAAGGTTGACCGCCCTGGCGCGCGTCCTGATTGGGTTGTGGATCAGGTATTCGATCTGTTCGTTAACCTCGACGCGACCGACG                >  SRR4302533.1960961/1‑94 (MQ=60)
                                                                                 TGTTATCAACAAGGTTGACCGCCCTGGCGCGCGTCCTGATTGGGTTGTGGATCAGGTATTCGATCTGTTCGTTAACCTCGACGCGACCGACGAG              <  SRR4302533.4087096/94‑1 (MQ=60)
                                                                                 TGTTATCAACAAGGTTGACCGCCCTGGCGCGCGTCCTGATTGGGTTGTGGATCAGGTATTCGATCTGTTCGTTAACCTCGACGCGACCGACGAG              <  SRR4302533.2096816/94‑1 (MQ=60)
                                                                                  GTTATCAACAAGGTTGACCGCCCTGGCGCGCGTCCTGATTGGGTTGTGGATCAGGTATTCGATCTGTTCGTTAACCTCGACGCGACCGACGAGC             <  SRR4302533.4078900/94‑1 (MQ=60)
                                                                                  GTTATCAACAAGGTTGACCGCCCTGGCGCGCGTCCTGATTGGGTTGTGGATCAGGTATTCGATCTGTTCGTTAACCTCGACGCGACCGACGAGC             <  SRR4302533.3073051/94‑1 (MQ=60)
                                                                                    TATCAACAAGGTTGACCGCCCTGGCGCGCGTCCTGATTGGGTTGTGGATCAGGTATTCGATCTGTTCGTTAACCTCGACGCGACCGACGAGCAG           <  SRR4302533.5947283/94‑1 (MQ=60)
                                                                                     ATCAACAAGGTTGACCGCCCTGGCGCGCGTCCTGATTGGGTTGTGGATCAGGTATTCGATCTGTTCGTTAACCTCGACGCGACCGACGAGCAGC          <  SRR4302533.4187105/94‑1 (MQ=60)
                                                                                      TCAACAAGGTTGACCGCCCTGGCGCGCGTCCTGATTGGGTTGTGGATCAGGTATTCGATCTGTTCGTTAACCTCGACGCGACCGACGAGCAGCT         <  SRR4302533.3163407/94‑1 (MQ=60)
                                                                                       CAACAAGGTTGACCGCCCTGGCGCGCGTCCTGATTGGGTTGTGGATCAGGTATTCGATCTGTTCGTTAACCTCGACGCGACCGACGAGCAGCTG        >  SRR4302533.3524943/1‑94 (MQ=60)
                                                                                       CAACAAGGTTGACCGCCCTGGCGCGCGTCCTGATTGGGTTGTGGATCAGGTATTCGATCTGTTCGTTAACCTCGACGCGACCGACGAGCAGCTG        >  SRR4302533.5253333/1‑94 (MQ=60)
                                                                                        AACAAGGTTGACCGCCCTGGCGCGCGTCCTGATTGGGTTGTGGATCAGGTATTCGATCTGTTCGTTAACCTCGACGCGACCGACGAGCAGCTGG       <  SRR4302533.2775364/94‑1 (MQ=60)
                                                                                          CAAGGTTGACCGCCCTGGCGCGCGTCCTGATTGGGTTGTGGATCAGGTATTCGATCTGTTCGTTAACCTCGACGCGACCGACGAGCAGCTGGAC     <  SRR4302533.3419213/94‑1 (MQ=60)
                                                                                           AAGGTTGACCGCCCTGGCGCGCGTCCTGATTGGGTTGTGGATCAGGTATTCGATCTGTTCGTTAACCTCGACGCGACCGACGAGCAGCTGGACT    <  SRR4302533.858002/94‑1 (MQ=60)
                                                                                             GGTTGACCGCCCTGGCGCGCGTCCTGATTGGGTTGTGGATCAGGTATTCGATCTGTTCGTTAACCTCGACGCGACCGACGAGCAGCTGGACTTC  >  SRR4302533.4042887/1‑94 (MQ=60)
                                                                                             |                                                                                             
GGTTGACGCATTTGACGGCCCGATGCCGCAAACGCGCTTCGTAACCAAAAAAGCGTTTGCTTACGGCCTGAAGCCGATTGTTGTTATCAACAAAGTTGACCGCCCTGGCGCGCGTCCTGATTGGGTTGTGGATCAGGTATTCGATCTGTTCGTTAACCTCGACGCGACCGACGAGCAGCTGGACTTC  >  NC_000913/4056280‑4056466

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑