Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1624	250.6	12620048	98.0%	12367647	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,117,363	G→A	C189C (TGC→TGT)	menA ←	MenA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,117,363	0	G	A	99.6%	852.1 / NA	231	C189C (TGC→TGT)	menA	MenA
Reads supporting (aligned to +/- strand): ref base G (0/1); new base A (142/88); total (142/89)
Fisher's exact test for biased strand distribution p-value = 3.85e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 233 total aligned reads displayed.

GCGCACCACCAGCGTGTTTTTGCCATTTTCGCGGTCGCTATTGATATCACGCAGGTTATTAATATTCAGTACTGCCGTTGCCAGCAGGCCGCATGCGGTCGCCGGAAGGATCAGTGCCGGAATCAATGTATGAGCCTGTAAATACCAGCTCCCCATGACACTCAACCAGCCAAAGAAAAC  >  NC_000913/4117273‑4117452

   caccacCAGCGTGTTTTTGCCATTTTCGCGGTCGCTATTGATATCACGCAGGTTATTAATATTCAGTACTGCCGTTGCCAGCAGGCCACATGc                                                                                      <  2:3030204/93‑1 (MQ=255)

    accaccAGCGTGTTTTTGCCAGTTTCGCGGTCGCTATTGATATCACGCAGGTTATTAATATTCAGTACTGCCGTTGCCAGCAGGCCACATGCg                                                                                     >  2:4008062/1‑93 (MQ=255)

       accaGCGTGTTTTTGCCATTTTCGCGGTCGCTATTGATATCACGCAGGTTATTAATATTCAGTACTGCCGTTGCCAGCAGGCCACATGCGGTc                                                                                  >  2:5087139/1‑93 (MQ=255)

       aacaGCGTGTTTTTGCCATTTTCGCGGTCGCTATTGATATCACGCAGGTTATTAATATTCAGTACTGCCGTTGCCAGCAGGCCACATGCGGTc                                                                                  >  1:4525719/3‑93 (MQ=255)

         caGCGTGTTTTTGCCATTTTCGCGGTCGCTATTGATATCACGCAGGTTATTAATATTCAGTACTGCCGTTGCCAGCAGGCCACATGCGGTCGc                                                                                >  2:1527366/1‑93 (MQ=255)

           gCGTGTTTTTGCCATTTTCGCGGTCGCTATTGATATCACGCAGGTTATTAATATTCAGTACTGCCGTTGCCAGCAGGCCACATGCGGTCGCCg                                                                              >  1:614308/1‑93 (MQ=255)

           gCGTGTTTTTGCCATTTTCGCGGTCGCTATTGATATCACGCAGGTTATTAATATTCAGTACTGCCGTTGCCAGCAGGCCACATGCGGTCGCCg                                                                              >  2:5866399/1‑93 (MQ=255)

           gCGTGTTTTTGCCATTTTCGCGGTCGCTATTGATATCACGCAGGTTATTAATATTCAGTACTGCCGTTGCCAGCAGGCCACATGCGGTCGCCg                                                                              <  1:4052865/93‑1 (MQ=255)

           gCGTGTTTTTGCCATTTTCGCGGTCGCTATTGATATCACGCAGGTTATTAATATTCAGTACTGCCGTTGCCAGCAGGCCACATGCGGTCGCCg                                                                              >  2:1189666/1‑93 (MQ=255)

           gCGTGTTTTTGCCATTTTCGCGGTCGCTATTGATATCACGCAGGTTATTAATATTCAGTACTGCCGTTGCCAGCAGGCCACATGCGGTCGCCg                                                                              <  2:4293495/93‑1 (MQ=255)

             gtgtTTTTGCCATTTTCGCGGTCGCTATTGATATCACGCAGGTTATTAATATTCAGTACTGCCGTTGCCAGCAGGCCACATGCGGTCGCCGGa                                                                            >  2:1555844/1‑93 (MQ=255)

             gtgtTTTTGCCATTTTCGCGGTCGCTATTGATATCACGCAGGTTATTAATATTCAGTACTGCCGTTGCCAGCAGGCCACATGCGGTCGCCGGa                                                                            >  1:1367761/1‑93 (MQ=255)

              tgtTTTTGCCATTTTCGCGGTCGCTATTGATATCACGCAGGTTATTAATATTCAGTACTGCCGTTGCCAGCAGGCCACATGCGGTCGCCGGaa                                                                           >  1:5897732/1‑93 (MQ=255)

               gtTTTTGCCATTTTCGCGGTCGCTATTGATATCACGCAGGTTATTAATATTCAGTACTGCCGTTGCCAGCAGGCCACATGCGGTCGCCGGAAg                                                                          >  2:4802009/1‑93 (MQ=255)

                tttttGCCATTTTCGCGGTCGCTATTGATATCACGCAGGTTATTAATATTCAGTACTGCCGTTGCCAGCAGGCCACATGCGGTCGCCGGAAgg                                                                         >  1:5159100/1‑93 (MQ=255)

                 ttttGCCATTTTCGCGTTCTCTATTGATATCACGCAGGTTATTAATATTCAGTACTGCCGTTGCCAGCAGGCCACATGCGGTCGCCGGAAGGa                                                                        <  1:4695511/93‑1 (MQ=255)

                 ttttGCCATTTTCGCGGTCGCTATTGATATCACGCAGGTTATTAATATTCAGTCCTGCCGTTGCCAGCAGGCCACATGCGGTCGCCGGAAGGa                                                                        <  2:2969951/93‑1 (MQ=255)

                 ttttGCCATTTTCGCGGTCGCTATTGATATCACGCAGGTTATTAATATTCAGTACTGCCGTTGCCAGCAGGCCACATGCGGTCGCCGGAAGGa                                                                        <  1:4625000/93‑1 (MQ=255)

                  tttGCCATTTTCGCGGTCGCTATTGATATCACGCAGGTTATTAATATTCAGTACTGCCGTTGCCAGCAGGCCACATGCGGTCGCCGGAAGGAt                                                                       <  2:5077001/93‑1 (MQ=255)

                    tGCCATTTTCGCGGTCGCTATTGATATCACGCAGGTTATTAATATTCAGTACTGCCGTTGCCAGCAGGCCACATGCGGTCGCCGGAAGGATCa                                                                     <  2:4912416/93‑1 (MQ=255)

                    tGCCATTTTCGCGGTCGCTATTGATATCACGCAGGTTATTAATATTCAGTACTGCCGTTGCCAGCAGGCCACATGCGGTCGCCGGAAGGATCa                                                                     >  1:5138785/1‑93 (MQ=255)

                    tGCCATTTTCGCGGTCGCTATTGATATCACGCAGGTTATTAATATTCAGTACTGCCGTTGCCAGCAGGCCACATGCGGTCGCCGGAAGGATCa                                                                     >  2:5443111/1‑93 (MQ=255)

                     gCCATTTTCGCGGTCGCTATTGATATCACGCAGGTTATTAATATTCAGTACTGCCGTTGCCAGCAGGCCACATGCGGTCGCCGGAAGGATCAg                                                                    <  2:2689859/93‑1 (MQ=255)

                     gCCATTTTCGCGGTCGCTATTGATATCACGCAGGTTATTAATATTCAGTACTGCCGTTGCCAGCAGGCCACATGCGGTCGCCGGAAGGATCAg                                                                    >  1:4913363/1‑93 (MQ=255)

                      ccATTTTCGCGGTCGCTATTGATATCACGCAGGTTATTAATATTCAGTACTGCCGTTGCCAGCAGGCCACATGCGGTCGCCGGAAGGATCAGt                                                                   <  2:6297349/93‑1 (MQ=255)

                      ccATTTTCGCGGTCGCTATTGATATCACGCAGGTTATTAATATTCAGTACTGCCGTTGCCAGCAGGCCACATGCGGTCGCCGGAAGGATCAGt                                                                   <  1:2115197/93‑1 (MQ=255)

                       cATTTTCGCGGTCGCTATTGATATCACGCAGGTTATTAATATTCAGTACTGCCGTTGCCAGCAGGCCACATGCGGTCGCCGGAAGGATCAGTg                                                                  >  2:3640640/1‑93 (MQ=255)

                        aTTTTCGCGGTCGCTATTGATATCACGCAGGTTATTAATATTCAGTACTGCCGTTGCCAGCAGGCCACATGCGGTCGCCGGAAGGATCAGTGc                                                                 >  2:3336085/1‑93 (MQ=255)

                        aTTTTCGCGGTCGCTACTGATATCACGCAGGTTATTAATATTCAGTACTGCCGTTGCCAGCAGGCCACATGCGGTCGCCGGAAGGATCAGTGc                                                                 <  2:4406659/93‑1 (MQ=255)

                         ttttCGCGGTCGCTATTGATATCACGCAGGTTATTAATATTCAGTACTGCCGTTGCCAGCAGGCCACATGCGGTCGCCGGAAGGATCAGTGcc                                                                >  1:357811/1‑93 (MQ=255)

                           ttCGCGGTCGCTATTGATATCACGCAGGTTATTAATATTCAGTACTGCCGTTGCCAGCAGGCCACATGCGGTCGCCGGAAGGATCAGTGCCgg                                                              <  2:2036361/93‑1 (MQ=255)

                           ttCGCGGTCGCTATTGATATCACGCAGGTTATTAATATTCAGTACTGCCGTTGCCAGCAGGCCACATGCGGTCGCCGGAAGGATCAGTGCCgg                                                              <  1:6297026/93‑1 (MQ=255)

                                ggTCGCTATTGATATCACGCAGGTTATTAATATTCAGTACTGCCGTTGCCAGCAGGCCACATGCGGTCGCCGGAAGGATCAGTGCCGGAATCa                                                         <  2:2393862/93‑1 (MQ=255)

                                ggTCGCTATTGATATCACGCAGGTTATTAATATTCAGTACTGCCGTTGCCAGCAGGCCACATGCGGTCGCCGGAAGGAACAGTGCCGGAATCa                                                         >  1:2604079/1‑93 (MQ=255)

                                 gTCGCTATTGATATCACGCAGGTTATTAATATTCAGTACTGCCGTTGCCAGCAGGCCACATGCGGTCGCCGGAAGGATCAGTGCCGGAATCaa                                                        <  2:321984/93‑1 (MQ=255)

                                 gTCGCTATTGATATCACGCAGGTTATTAATATTCAGTACTGCCGTTGCCAGCAGGCCACATGCGGTCGCCGGAAGGATCAGTGCCGGAATCaa                                                        >  2:3389414/1‑93 (MQ=255)

                                  tCGCTATTGATATCACGCAGGTTATTAATATTCAGTACTGCCGTTGCCAGCAGGCCACATGCGGTCGCCGGAAGGATCAGTGCCGGAATCAAt                                                       <  1:5646833/93‑1 (MQ=255)

                                   cGCTATTGATATCACGCAGGTTATTAATATTCAGTACTGCCGTTGCCAGCAGGCCACATGCGGTCGCCGGAAGGATCAGTGCCGGAATCAATg                                                      <  2:2443691/93‑1 (MQ=255)

                                    gCTATTGATATCACGCAGGTTATTAATATTCAGTACTGCCGTTGCCAGCAGGCCACATGCGGTCGCCGGAAGGATCAGTGGCGGAATCTATga                                                     >  2:4094260/1‑92 (MQ=255)

                                    gCTATTGATATCACGCAGGTTATTAATATTCAGTACTGCCGTTGCCAGCAGGCCACATGCGGTCGCCGGAAGGATCAGTGCCGGAATCAATGt                                                     >  1:3894304/1‑93 (MQ=255)

                                      tATTGATATCACGCAGGTTATTAATATTCAGTACTGCCGTTGCCAGCAGGCCACATGCGGTCGCCGGAAGGATCAGTGCCGGAATCAATGTAt                                                   >  1:5847624/1‑93 (MQ=255)

                                      tATTGATATCACGCAGGTTATTAATATTCAGTACTGCCGTTGCCAGCAGGCCACATGCGGTCGCCGGAAGGATCAGTGCCGGAATCAATGTAt                                                   <  1:787189/93‑1 (MQ=255)

                                       aTTGATATCACGCAGGTTATTAATATTCAGTACTGCCGTTGCCAGCAGGCCACATGCGGTCGCCGGAAGGATCAGTGCCGGAATCAATGTATg                                                  >  2:3537649/1‑93 (MQ=255)

                                       aTTGATATCACGCAGGTTATTAATATTCAGTACTGCCGTTGCCAGCAGGCCACATGCGGTCGCCGGAAGGATCAGTGCCGGAATCAATGTATg                                                  <  1:2850410/93‑1 (MQ=255)

                                       aTTGATATCACGCAGGTTATTAATATTCAGTACTGCCGTTGCCAGCAGGCCACATGCGGTCGCCGGAAGGATCAGTGCCGGAATCAATGTATg                                                  >  1:6155176/1‑93 (MQ=255)

                                        ttGATATCACGCAGGTTATTAATATTCAGTACTGCCGTTGCCAGCAGGCCACATGCGGTCGCCGGAAGGATCAGTGCCGGAATCAATGTATGa                                                 >  2:665253/1‑93 (MQ=255)

                                        ttGATATCACGCAGGTTATTAATATTCAGTACTGCCGTTGCCAGCAGGCCACATGCGGTCGCCGGAAGGATCAGTGCCGGAATCAATGTATGa                                                 <  1:3673733/93‑1 (MQ=255)

                                            tatCACGCAGGTTATTAATATTCAGTACTGCCGTTGCCAGCAGGCCACATGCGGTCGCCGGAAGGATCAGTGCCGGAATCAATGTATGAGCCt                                             >  2:4495336/1‑93 (MQ=255)

                                              tCACGCAGGTTATTAATATTCAGTACTGCCGTTGCCAGCAGGCCACATGCGGTCGCCGGAAGGATCAGTGCCGGAATCAATGTATGAGCCTGt                                           <  1:5795693/93‑1 (MQ=255)

                                              tCACGCAGGTTATTAATATTCAGTACTGCCGTTGCCAGCAGGCCACATGCGGTCGCCGGAAGGATCAGTGCCGGAATCAATGTATGAGCCTGt                                           >  2:1275279/1‑93 (MQ=255)

                                               cACGCAGGTTATTAATATTCAGTACTGCCGTTGCCAGCAGGCCACATGCGGTCGCCGGAAGGATCAGTGCCGGAATCAATGTATGAGCCTGTa                                          >  2:1870061/1‑93 (MQ=255)

                                               cACGCAGGTTATTAATATTCAGTACTGCCGTTGCCAGCAGGCCACATGCGGTCGCCGGAAGGATCAGTGCCGGAATCAATGTATGAGCCTGTa                                          <  2:3032547/93‑1 (MQ=255)

                                                 cGCAGGTTATTAATATTCAGTACTGCCGTTGCCAGCAGGCCACATGCGGTCGCCGGAAGGATCAGTGCCGGAATCAATGTATGAGCCTGTaaa                                        <  2:2555202/93‑1 (MQ=255)

                                                 cGCAGGTTATTAATATTCAGTACTGCCGTTGCCAGCAGGCCACATGCGGTCGCCGGAAGGATCAGTGCCGGAATCAATGTATGAGCCTGTaaa                                        >  2:830260/1‑93 (MQ=255)

                                                   cAGGTTATTAATATTCAGTACTGCCGTTGCCAGCAGGCCACATGCGGTCGCCGGAAGGATCAGTGCCGGAATCAATGTATGAGCCTGTAAATa                                      <  1:3479592/93‑1 (MQ=255)

                                                    aGGTTATTAATATTCAGTACTGCCGTTGCCAGCAGGCCACATGCGGTCGCCGGAAGGATCAGTGCCGGAATCAATGTATGAGCCTGTAAATAc                                     >  1:2867360/1‑93 (MQ=255)

                                                    aGGTTATTAATATTCAGTACTGCCGTTGCCAGCAGGCCACATGCGGTCGCCGGAAGGATCAGTGCCGGAATCAATGTATGAGCCTGTAAATAc                                     <  2:4829155/93‑1 (MQ=255)

                                                     ggTTATTAATATTCAGTACTGCCGTTGCCAGCAGGCCACATGCGGTCGCCGGAAGGATCAGTGCCGGAATCAATGTATGAGCCTGTAAATAcc                                    >  2:3789263/1‑93 (MQ=255)

                                                     ggTTATTAATATTCAGTACTGCCGTTGCCAGCAGGCCACATGCGGTCGCCGGAAGGATCAGTGCCGGAATCAATGTATGAGCCTGTAAATAcc                                    >  1:4202839/1‑93 (MQ=255)

                                                     ggTTATTAATATTCAGTACTGCCGTTGCCAGCAGGCCACATGCGGTCGCCGGAAGGATCAGTGCCGGAATCAATGTATGAGCCTGTAAATAcc                                    >  1:1481993/1‑93 (MQ=255)

                                                     ggTTATTAATATTCAGTACTGCCGTTGCCAGCAGGCCACATGCGGTCGCCGGAAGGATCAGTGCCGGAATCAATGTATGAGCCTGTAAATAcc                                    >  1:5638346/1‑93 (MQ=255)

                                                     ggTTATTAATATTCAGTACTGCCGTTGCCAGCAGGCCACATGCGGTCGCCGGAAGGATCAGTGCCGGAATCAATGTATGAGCCTGTAAATAcc                                    <  2:5691650/93‑1 (MQ=255)

                                                      gTTATTAATATTCAGTACTGCCGTTGCCAGCAGGCCACATGCGGTCGCCGGAAGGATCAGTGCCGGAATCAATGTATGAGCCTGTAAATACCa                                   <  2:5339417/93‑1 (MQ=255)

                                                      gTTATTAATATTCAGTACTGCCGTTGCCAGCAGGCCACATGCGGTCGCCGGAAGGATCAGTGCCGGAATCAATGTATGAGCCTGTAAATACCa                                   >  1:4721273/1‑93 (MQ=255)

                                                        tattaATATTCAGTACTGCCGTTGCCAGCAGGCCACATGCGGTCGCCGGAAGGATCAGTGCCGGAATCAATGTATGAGCCTGTAAATACCAGc                                 <  1:4083032/93‑1 (MQ=255)

                                                         attaATATTCAGTACTGCCGTTGCCAGCAGGCCACATGCGGTCGCCGGAAGGATCAGTGCCGGAATCAATGTATGAGCCTGTAAATACCAGCt                                >  1:3163962/1‑93 (MQ=255)

                                                         attaATATTCAGTACTGCCGTTGCCAGCAGGCCACATGCGGTCGCCGGAAGGATCAGTGCCGGAATCAATGTATGAGCCTGTAAATACCAGCt                                >  1:6269266/1‑93 (MQ=255)

                                                          ttaATATTCAGTACTGCCGTTGCCAGCAGGCCACATGCGGTCGCCGGAAGGATCAGTGCCGGAATCAATGTATGAGCCTGTAAATACCAGCTc                               >  2:2267790/1‑93 (MQ=255)

                                                            aaTATTCAGTACTGCCGTTGCCAGCAGGCCACATGCGGTCGCCGGAAGGATCAGTGCCGGAATCAATGTATGAGCCTGTAAATACCAGCTccc                             >  2:779293/1‑93 (MQ=255)

                                                             atatTCAGTACTGCCGTTGCCAGCAGGCCACATGCGGTCGCCGGAAGGATCAGTGCCGGAATCAATGTATGAGCCTGTAAATACCAGCTcccc                            <  2:2513182/93‑1 (MQ=255)

                                                              tatTCAGTACTGCCGTTGCCAGCAGGCCACATGCGGTCGCCGGAAGGATCAGTGCCGGAATCAATGTATGAGCCTGTAAATACCAGCTCCCCa                           <  1:1662545/93‑1 (MQ=255)

                                                              tatTCAGTACTGCCGTTGCCAGCAGGCCACATGCGGTCGCCGGAAGGATCAGTGCCGGAATCAATGTATGAGCCTGTAAATACCAGCTCCCCa                           >  2:3582046/1‑93 (MQ=255)

                                                               atTCAGTACTGCCGTTGCCAGCAGGCCACATGCGGTCGCCGGAAGGATCAGTGCCGGAATCAATGTATGAGCCTGTAAATACCAGCTCCCCAt                          >  1:1601089/1‑93 (MQ=255)

                                                                ttCAGTACTGCCGTTGCCAGCAGGCCACATGCGGTCGCCGGAAGGATCAGTGCCGGAATCAATGTATGAGCCTGTAAATACCAGCTCCCCATg                         >  1:2904640/1‑93 (MQ=255)

                                                                 tCAGTACTGCCGTTGCCAGCAGGCCACATGCGGTCGCCGGAAGGATCAGTGCCGGAATCAATGTATGAGCCTGTAAATACCAGCTCCCCATGa                        <  1:3089028/93‑1 (MQ=255)

                                                                  cAGTACTGCCGTTGCCAGCAGGCCACATGCGGTCGCCGGAAGGATCAGTGCCGGAATCAATGTATGAGCCTGTAAATACCAGCTCCCCATGac                       <  1:1038219/93‑1 (MQ=255)

                                                                  cAGTACTGCCGTTGCCAGCAGGCCACATGCGCTCGCCGGAAGGATCAGTCCCGGAATCAATGTATGAGCCTGTAAATACCAGCTCCCCATGac                       <  2:4441497/93‑1 (MQ=255)

                                                                   aGTACTGCCGTTGCCAGCAGGCCACATGCGGTCGCCGGAAGGATCAGTGCCGGAATCAATTTATGAGCCTGTAAATACCAGCTCCCCATGaca                      >  1:2431199/1‑93 (MQ=255)

                                                                    gTACTGCCGTTGCCAGCAGGCCACATGCGGTCGCCGGAAGGATCAGTGCCGGAATCAATGTATGAGCCTGTAAATACCAGCTCCCCATGacac                     <  1:6199829/93‑1 (MQ=255)

                                                                      aCTGCCGTTGCCAGCAGGCGACATGCGGTCGCCGGAAGGATCAGTGCCGGAATCAATGTATGAGCCTGTAAATACCAGCTCCCCATGACACTc                   >  2:6166144/1‑93 (MQ=255)

                                                                       cTGCCGTTGCCAGCAGGCCACATGCGGTCGCCGGAAGGATCAGTGCCGGAATCAATGTATGAGCCTGTAAATACCAGCTCCCCATGACACTCa                  >  2:3488098/1‑93 (MQ=255)

                                                                       cTGCCGTTGCCAGCAGGCCACATGCGGTCGCCGGAAGGATCAGTGCCGGAATCAATGTATGAGCCTGTAAATACCAGCTCCCCATGACACTCa                  >  1:435380/1‑93 (MQ=255)

                                                                       cTGCCGTTGCCAGCAGGCCACATGCGGTCGCCGGAAGGATCAGTGCCGGAATCAATGTATGAGCCTGTAAATACCAGCTCCCCATGACACTCa                  >  2:4109882/1‑93 (MQ=255)

                                                                        tGCCGTTGCCAGCAGGCCACATGCGGTCGCCGGAAGGATCAGTGCCGGAATCAATGTATGAGCCTGTAAATACCAGCTCCCCATGACACTCaa                 <  1:128241/93‑1 (MQ=255)

                                                                        tGCCGTTGCCAGCAGGCCACATGCGGTCGCCGGAAGGATCAGTGCCGGAATCAATGTATGAGCCTGTAAATACCAGCTCCCCATGACACTCaa                 >  1:521983/1‑93 (MQ=255)

                                                                        tGCCGTTGCCAGCAGGCCACATGCGGTCGCCGGAAGGATCAGTGCCGGAATCAATGTATGAGCCTGTAAATACCAGCTCCCCATGACACTCaa                 >  2:277914/1‑93 (MQ=255)

                                                                         gCCGTTGCCAGCAGGCCACATGCGGTCGCCGGAAGGATCAGTGCCGGAATCAATGTATGAGCCTGTAAATACCAGCTCCCCATGACACTCAAc                >  2:1752256/1‑93 (MQ=255)

                                                                         gCCGTTGCCAGCAGGCCACATGCGGTCGCCGGAAGGATCAGTGCCGGAATCAATGTATGAGCCTGTAAATACCAGCTCCCCATGACACTCAAc                >  2:5358745/1‑93 (MQ=255)

                                                                           cGTTGCCAGCAGGCCACATGCGGTCGCCGGAAGGATCAGTGCCGGAATCAATGTATGAGCCTGTAAATACCAGCTCCCCATGACACTCAACCa              <  1:2622747/93‑1 (MQ=255)

                                                                           cGTGGCAAGCAGGCCACATGCGGTCGCCGGAAGGATCAGTGCCGGAATCAATGTATGAGCCTGTAAATACCAGCTCCCCATGACACTCAACCa              <  2:3266804/93‑1 (MQ=255)

                                                                             ttGCCAGCAGGCCACATGCGGTCGCCGGAAGGATCAGTGCCGGAATCAATGTATGAGCCTGTAAATACCAGCTCCCCATGACACTCAACCAGc            >  1:1943011/1‑93 (MQ=255)

                                                                             ttGCCAGCAGGCCACATGCGGTCGCCGGAAGGATCAGTGCCGGAATCAATGTATGAGCCTGTAAATACCAGCTCCCCATGACACTCAACCAGc            >  1:6085098/1‑93 (MQ=255)

                                                                             ttGCCAGCAGGCCACATGCGGTCGCCGGAAGGATCAGTGCCGGAATCAATGTATGAGCCTGTAAATACCAGCTCCCCATGACACTCAACCAGc            >  1:4414347/1‑93 (MQ=255)

                                                                                 cagcagGCCACATGCGGTCGCCGGAAGGATCAGTGCCGGAATCAATGTATGAGCCTGTAAATACCAGCTCCCCATGACACTCAACCAGCCaaa        >  2:599307/1‑93 (MQ=255)

                                                                                  agcagGCCACATGCGGTCGCCGGAAGGATCAGTGCCGGAATCAATGTATGAGCCTGTAAATACCAGCTCCCCATGACACTCAACCAGCCAAAg       >  1:2309283/1‑93 (MQ=255)

                                                                                  agcagGCCACATGCGGTCGCCGGAAGGATCAGTGCCGGAATCAATGTATGAGCCTGTAAATACCAGCTCCCCATGACACTCAACCAGCCAAAg       <  1:3279389/93‑1 (MQ=255)

                                                                                   gcagGCCACATGCGGTCGCCGGAAGGATCAGTGCCGGAATCAATGTATGAGCCTGTAAATACCAGCTCCCCATGACACTCAACCAGCCAAAGa      >  1:5395955/1‑93 (MQ=255)

                                                                                    cagGCCACATGCGGTCGCCGGAAGGATCAGTGCCGGAATCAATGTATGAGCCTGTAAATACCAGCTCCCCATGACACTCAACCAGCCAAAGaa     <  1:4863293/93‑1 (MQ=255)

                                                                                      ggCCACATGCGGTCGCCGGAAGGATCAGTGCCGGAATCAATGTATGAGCCTGTAAATACCAGCTCCCCATGACACTCAACCAGCCAAAGaaac   >  1:2092155/1‑92 (MQ=255)

                                                                                      ggCCACATGCGGTCGCCGGAAGGATCAGTGCCGGAATCAATGTATGAGCCTGTAAATACCAGCTCCCCATGACACTCAACCAGCCAAAGaaaa   >  2:6185654/1‑93 (MQ=255)

GCGCACCACCAGCGTGTTTTTGCCATTTTCGCGGTCGCTATTGATATCACGCAGGTTATTAATATTCAGTACTGCCGTTGCCAGCAGGCCGCATGCGGTCGCCGGAAGGATCAGTGCCGGAATCAATGTATGAGCCTGTAAATACCAGCTCCCCATGACACTCAACCAGCCAAAGAAAAC  >  NC_000913/4117273‑4117452

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

TAAGCGCACCACCAGCGTGTTTTTGCCATTTTCGCGGTCGCTATTGATATCACGCAGGTTATTAATATTCAGTACTGCCGTTGCCAGCAGGCCGCATGCGGTCGCCGGAAGGATCAGTGCCGGAATCAATGTATGAGCCTGTAAATACCAGCTCCCCATGACACTCAACCAGCCAAAGAAAACCAGT  >  NC_000913/4117270‑4117456

TAAGCGCACCACCAGCGTGTTTTTGCCATTTTCGCGGTCGCTATTGATATCACGCAGGTTATTAATATTCAGTACTGCCGTTGCCAGCAGGCCA                                                                                               <  SRR4302533.2649198/94‑1 (MQ=60)

  AGCGCACCACCAGCGTGTTTTTGCCATTTTCGCGGTCGCTATTGATATCACGCAGGTTATTAATATTCAGTACTGCCGTTGCCAGCAGGCCACA                                                                                             <  SRR4302533.6305115/94‑1 (MQ=60)

     GCACCACCAGCGTGTTTTTGCCATTTTCGCGGTCGCTATTGATATCACGCAGGTTATTAATATTCAGTACTGCCGTTGCCAGCAGGCCACATGC                                                                                          >  SRR4302533.2760233/1‑94 (MQ=60)

     GCACCACCAGCGTGTTTTTGCCATTTTCGCGGTCGCTATTGATATCACGCAGGTTATTAATATTCAGTACTGCCGTTGCCAGCAGGCCACATGC                                                                                          <  SRR4302533.3801647/94‑1 (MQ=60)

      CACCACCAGCGTGTTTTTGCCATTTTCGCGGTCGCTATTGATATCACGCAGGTTATTAATATTCAGTACTGCCGTTGCCAGCAGGCCACATGCG                                                                                         <  SRR4302533.4705405/94‑1 (MQ=60)

       ACCACCAGCGTGTTTTTGCCATTTTCGCGGTCGCTATTGATATCACGCAGGTTATTAATATTCAGTACTGCCGTTGCCAGCAGGCCACATGCGG                                                                                        <  SRR4302533.1579054/94‑1 (MQ=60)

        CCACCAGCGTGTTTTTGCCATTTTCGCGGTCGCTATTGATATCACGCAGGTTATTAATATTCAGTACTGCCGTTGCCAGCAGGCCACATGCGGC                                                                                       >  SRR4302533.1949232/1‑94 (MQ=60)

         CACCAGCGTGTTTTTGCCATTTTCGCGGTCGCTATTGATATCACGCAGGTTATTAATATTCAGTACTGCCGTTGCCAGCAGGCCACATGCGGTC                                                                                      >  SRR4302533.5010142/1‑94 (MQ=60)

         CAACAGCGTGTTTTTGCCATTTTCGCGGTCGCTATTGATATCACGCAGGTTATTAATATTCAGTACTGCCGTTGCCAGCAGGCCACATGCGGTC                                                                                      >  SRR4302533.4553120/1‑94 (MQ=60)

             AGCGTGTTTTTGCCATTTTCGCGGTCGCTATTGATATCACGCAGGTTATTAATATTCAGTACTGCCGTTGCCAGCAGGCCACATGCGGTCGCCG                                                                                  <  SRR4302533.1430893/94‑1 (MQ=60)

             AGCGTGTTTTTGCCATTTTCGCGGTCGCTATTGATATCACGCAGGTTATTAATATTCAGTACTGCCGTTGCCAGCAGGCCACATGCGGTCGCCG                                                                                  <  SRR4302533.6215130/94‑1 (MQ=60)

             AGCGTGTTTTTGCCATTTTCGCGGTCGCTATTGATATCACGCAGGTTATTAATATTCAGTACTGCCGTTGCCAGCAGGCCACATGCGGTCGCCG                                                                                  >  SRR4302533.617919/1‑94 (MQ=60)

              GCGTGTTTTTGCCATTTTCGCGGTCGCTATTGATATCACGCAGGTTATTAATATTCAGTACTGCCGTTGCCAGCAGGCCACATGCGGTCGCCGG                                                                                 <  SRR4302533.4077546/94‑1 (MQ=60)

               CGTGTTTTTGCCATTTTCGCGGTCGCTATTGATATCACGCAGGTTATTAATATTCAGTACTGCCGTTGCCAGCAGGCCACATGCGGTCGCCGGA                                                                                >  SRR4302533.1375986/1‑94 (MQ=60)

                GTGTTTTTGCCATTTTCGCGGTCGCTATTGATATCACGCAGGTTATTAATATTCAGTACTGCCGTTGCCAGCAGGCCACATGCGGTCGCCGGAA                                                                               >  SRR4302533.5933351/1‑94 (MQ=60)

                GTGTTTTTGCCATTTTCGCGGTCGCTATTGATATCACGCAGGTTATTAATATTCAGTACTGCCGTTGCCAGCAGGCCACATGCGGTCGCCGGAA                                                                               >  SRR4302533.3300087/1‑94 (MQ=60)

                  GTTTTTGCCATTTTCGCGGTCGCTATTGATATCACGCAGGTTATTAATATTCAGTACTGCCGTTGCCAGCAGGCCACATGCGGTCGCCGGAAGG                                                                             >  SRR4302533.5190241/1‑94 (MQ=60)

                   TTTTTGCCATTTTCGCGGTCGCTATTGATATCACGCAGGTTATTAATATTCAGTACTGCCGTTGCCAGCAGGCCACATGCGGTCGCCGGAAGGA                                                                            >  SRR4302533.3272942/1‑94 (MQ=60)

                    TTTTGCCATTTTCGCGTTCTCTATTGATATCACGCAGGTTATTAATATTCAGTACTGCCGTTGCCAGCAGGCCACATGCGGTCGCCGGAAGGAT                                                                           <  SRR4302533.4723966/94‑1 (MQ=60)

                    TTTTGCCATTTTCGCGGTCGCTATTGATATCACGCAGGTTATTAATATTCAGTACTGCCGTTGCCAGCAGGCCACATGCGGTCGCCGGAAGGAT                                                                           <  SRR4302533.4653034/94‑1 (MQ=60)

                    TTTTGCCATTTTCGCGGTCGCTATTGATATCACGCAGGTTATTAATATTCAGTACTGCCGTTGCCAGCAGGCCACATGCGGTCGCCGGAAGGAT                                                                           >  SRR4302533.5304668/1‑94 (MQ=60)

                    TGTTGCCATTTTCGCGGTCGCTATTGATATCACGCAGGTTATTAATATTCAGTACTGCCGTTGCCAGCAGGCCACATGCGGTCGCCGGAAGGAT                                                                           <  SRR4302533.4723967/94‑1 (MQ=60)

                      TTGCCATTTTCGCGGTCGCTATTGATATCACGCAGGTTATTAATATTCAGTACTGCCGTTGCCAGCAGGCCACATGCGGTCGCCGGAAGGATCA                                                                         <  SRR4302533.2398449/94‑1 (MQ=60)

                      TTGCCATTTTCGCGGTCGCTATTGATATCACGCAGGTTATTAATATTCAGTACTGCCGTTGCCAGCAGGCCACATGCGGTCGCCGGAAGGATCA                                                                         >  SRR4302533.5169841/1‑94 (MQ=60)

                       TGCCATTTTCGCGGTCGCTATTGATATCACGCAGGTTATTAATATTCAGTACTGCCGTTGCCAGCAGGCCACATGCGGTCGCCGGAAGGATCAG                                                                        <  SRR4302533.3935794/94‑1 (MQ=60)

                       TGCCATTTTCGCGGTCGCTATTGATATCACGCAGGTTATTAATATTCAGTACTGCCGTTGCCAGCAGGCCACATGCGGTCGCCGGAAGGATCAG                                                                        >  SRR4302533.4943123/1‑94 (MQ=60)

                       TGCCATTTTCGCGGTCGCTATTGATATCACGCAGGTTATTAATATTCAGTACTGCCGTTGCCAGCAGGCCACATGCGGTCGCCGGAAGGATCAG                                                                        >  SRR4302533.6338281/1‑94 (MQ=60)

                       TGCCATTTTCGCGGTCGCTATTGATATCACGCAGGTTATTAATATTCAGTACTGCCGTTGCCAGCAGGCCACATGCGGTCGCCGGAAGGATCAG                                                                        <  SRR4302533.354516/94‑1 (MQ=60)

                        GCCATTTTCGCGGTCGCTATTGATATCACGCAGGTTATTAATATTCAGTACTGCCGTTGCCAGCAGGCCACATGCGGTCGCCGGAAGGATCAGT                                                                       >  SRR4302533.4349501/1‑94 (MQ=60)

                         CCATTTTCGCGGTCGCTATTGATATCACGCAGGTTATTAATATTCAGTACTGCCGTTGCCAGCAGGCCACATGCGGTCGCCGGAAGGATCAGTG                                                                      <  SRR4302533.2128242/94‑1 (MQ=60)

                         CCATTTTCGCGGTCGCTATTGATATCACGCAGGTTATTAATATTCAGTACTGCCGTTGCCAGCAGGCCACATGCGGTCGCCGGAAGGATCAGTG                                                                      >  SRR4302533.5175227/1‑94 (MQ=60)

                           ATTTTCGCGGTCGCTATTGATATCACGCAGGTTATTAATATTCAGTACTGCCGTTGCCAGCAGGCCACATGCGGTCGCCGGAAGGATCAGTGCC                                                                    >  SRR4302533.359989/1‑94 (MQ=60)

                           ATTTTCGCGGTCGCTATTGATATCACGCAGGTTATTAATATTCAGTACTGCCGTTGCCAGCAGGCCACATGCGGTCGCCGGAAGGATCAGTGCC                                                                    >  SRR4302533.6184835/1‑94 (MQ=60)

                             TTTCACGGTCGCTATTGATATCACTCAGGTTATTAATATTCAGTACTGCCGTTGCCAGCAGGCCGCATGCGGTCGCCGGAAGGATCAGTGCCGG                                                                  <  SRR4302533.820357/94‑1 (MQ=60)

                              TTCGCGGTCGCTATTGATATCACGCAGGTTATTAATATTCAGTACTGCCGTTGCCAGCAGGCCACATGCGGTCGCCGGAAGGATCAGTGCCGGA                                                                 <  SRR4302533.6334603/94‑1 (MQ=60)

                               TCGCGGTCGCTATTGATATCACGCAGGTTATTAATATTCAGTACTGCCGTTGCCAGCAGGCCACATGCGGTCGCCGGAAGGATCAGTGCCGGAA                                                                <  SRR4302533.4583452/94‑1 (MQ=60)

                                  CGGTCGCTATTGATATCACGCAGGTTATTAATATTCAGTACTGCCGTTGCCAGCAGGCCACATGCGGTCGCCGGAAGGATCAGTGCCGGAATCA                                                             >  SRR4302533.5713713/1‑94 (MQ=60)

                                  CGGTCGCTATTGATATCACGCAGGTTATTAATATTCAGTACTGCCGTTGCCAGCAGGCCACATGCGGTCGCCGGAAGGAACAGTGCCGGAATCA                                                             >  SRR4302533.2620076/1‑94 (MQ=60)

                                    GTCGCTATTGATATCACGCAGGTTATTAATATTCAGTACTGCCGTTGCCAGCAGGCCACATGCGGTCGCCGGAAGGATCAGTGCCGGAATCAAT                                                           >  SRR4302533.300183/1‑94 (MQ=60)

                                    GTCGCTATTGATATCACGCAGGTTATTAATATTCAGTACTGCCGTTGCCAGCAGGCCACATGCGGTCGCCGGAAGGATCAGTGCCGGAATCAAT                                                           >  SRR4302533.4174641/1‑94 (MQ=60)

                                     TCGCTATTGATATCACGCAGGTTATTAATATTCAGTACTGCCGTTGCCAGCAGGCCACATGCGGTCGCCGGAAGGATCAGTGCCGGAATCAATG                                                          <  SRR4302533.5680970/94‑1 (MQ=60)

                                      CGCTATTGATATCACGCAGGTTATTAATATTCAGTACTGCCGTTGCCAGCAGGCCACATGCGGTCGCCGGAAGGATCAGTGCCGGAATCAATGT                                                         >  SRR4302533.3918068/1‑94 (MQ=60)

                                       GCTATTGATATCACGCAGGTTATTAATATTCAGTACTGCCGTTGCCAGCAGGCCACATGCGGTCGCCGGAAGGATCAGTGCCGGAATCAATGTA                                                        >  SRR4302533.523596/1‑94 (MQ=60)

                                       GCTATTGATATCACGCAGGTTATTAATATTCAGTACTGCCGTTGCCAGCAGGCCACATGCGGTCGCCGGAAGGATCAGTGCCGGAATCAATGTA                                                        <  SRR4302533.4821996/94‑1 (MQ=60)

                                       GCTATTGATATCACGCAGGTTATTAATATTCAGTACTGCCGTTGCCAGCAGGCCACATGCGGTCGCCGGAAGGATCAGTGCCGGAATCAATGTA                                                        >  SRR4302533.4903097/1‑94 (MQ=60)

                                        CTATTGATATCACGCAGGTTATTAATATTCAGTACTGCCGTTGCCAGCAGGCCACATGCGGTCGCCGGAAGGATCAGTGCCGGAATCAATGTAT                                                       >  SRR4302533.5882973/1‑94 (MQ=60)

                                         TATTGATATCACGCAGGTTATTAATATTCAGTACTGCCGTTGCCAGCAGGCCACATGCGGTCGCCGGAAGGATCAGTGCCGGAATCAATGTATG                                                      >  SRR4302533.3486401/1‑94 (MQ=60)

                                         TATTGATATCACGCAGGTTATTAATATTCAGTACTGCCGTTGCCAGCAGGCCACATGCGGTCGCCGGAAGGATCAGTGCCGGAATCAATGTATG                                                      >  SRR4302533.6192207/1‑94 (MQ=60)

                                         TATTGATATCACGCAGGTTATTAATATTCAGTACTGCCGTTGCCAGCAGGCCACATGCGGTCGCCGGAAGGATCAGTGCCGGAATCAATGTATG                                                      <  SRR4302533.791814/94‑1 (MQ=60)

                                          ATTGATATCACGCAGGTTATTAATATTCAGTACTGCCGTTGCCAGCAGGCCACATGCGGTCGCCGGAAGGATCAGTGCCGGAATCAATGTATGA                                                     <  SRR4302533.2867948/94‑1 (MQ=60)

                                          ATTGATATCACGCAGGGTATTAATATTCAGTGCTGCCGTTGCCAGCAGGCCACATGCGGTCGCCGGAAGGATCAGTGCCGGAATCAATGTATGA                                                     <  SRR4302533.2867949/94‑1 (MQ=60)

                                           TTGATATCACGCAGGTTATTAATATTCAGTACTGCCGTTGCCAGCAGGCCACATGCGGTCGCCGGAAGGATCAGTGCCGGAATCAATGTATGAG                                                    <  SRR4302533.1961686/94‑1 (MQ=60)

                                           TTGATATCACGCAGGTTATTAATATTCAGTACTGCCGTTGCCAGCAGGCCACATGCGGTCGCCGGAAGGATCAGTGCCGGAATCAATGTATGAG                                                    <  SRR4302533.3696309/94‑1 (MQ=60)

                                               TATCACGCAGGTTATTAATATTCAGTACTGCCGTTGCCAGCAGGCCACATGCGGTCGCCGGAAGGATCAGTGCCGGAATCAATGTATGAGCCTG                                                >  SRR4302533.292220/1‑94 (MQ=60)

                                                ATCACGCAGGTTATTAATATTCAGTACTGCCGTTGCCAGCAGGCCACATGCGGTCGCCGGAAGGATCAGTGCCGGAATCAATGTATGAGCCTGT                                               >  SRR4302533.1656968/1‑94 (MQ=60)

                                                 TCACGCAGGTTATTAATATTCAGTACTGCCGTTGCCAGCAGGCCACATGCGGTCGCCGGAAGGATCAGTGCCGGAATCAATGTATGAGCCTGTA                                              <  SRR4302533.2216212/94‑1 (MQ=60)

                                                 TCACGCAGGTTATTAATATTCAGTACTGCCGTTGCCAGCAGGCCACATGCGGTCGCCGGAAGGATCAGTGCCGGAATCAATGTATGAGCCTGTA                                              <  SRR4302533.5830709/94‑1 (MQ=60)

                                                  CACGCAGGTTATTAATATTCAGTACTGCCGTTGCCAGCAGGCCACATGCGGTCGCCGGAAGGATCAGTGCCGGAATCAATGTATGAGCCTGTAA                                             >  SRR4302533.6309489/1‑94 (MQ=60)

                                                  CACGCAGGTTATTAATATTCAGTACTGCCGTTGCCAGCAGGCCACATGCGGTCGCCGGAAGGATCAGTGCCGGAATCAATGTATGAGCCTGTAA                                             >  SRR4302533.1367088/1‑94 (MQ=60)

                                                    CGCAGGTTATTAATATTCAGTACTGCCGTTGCCAGCAGGCCACATGCGGTCGCCGGAAGGATCAGTGCCGGAATCAATGTATGAGCCTGTAAAT                                           <  SRR4302533.2582579/94‑1 (MQ=60)

                                                     GCAGGTTATTAATATTCAGTACTGCCGTTGCCAGCAGGCCACATGCGGTCGCCGGAAGGATCAGTGCCGGAATCAATGTATGAGCCTGTAAATA                                          >  SRR4302533.4731270/1‑94 (MQ=60)

                                                      CAGGTTATTAATATTCAGTACTGCCGTTGCCAGCAGGCCACATGCGGTCGCCGGAAGGATCAGTGCCGGAATCAATGTATGAGCCTGTAAATAC                                         >  SRR4302533.2885002/1‑94 (MQ=60)

                                                      CAGGTTATTAATATTCAGTACTGCCGTTGCCAGCAGGCCACATGCGGTCGCCGGAAGGATCAGTGCCGGAATCAATGTATGAGCCTGTAAATAC                                         >  SRR4302533.2813408/1‑94 (MQ=60)

                                                      CAGGTTATTAATATTCAGTACTGCCGTTGCCAGCAGGCCACATGCGGTCGCCGGAAGGATCAGTGCCGGAATCAATGTATGAGCCTGTAAATAC                                         <  SRR4302533.3501061/94‑1 (MQ=60)

                                                      CAGGTTATTAATATTCAGTACTGCCGTTGCCAGCAGGCCACATGCGGTCGCCGGAAGGATCAGTGCCGGAATCAATGTATGAGCCTGTAAATAC                                         <  SRR4302533.3046280/94‑1 (MQ=60)

                                                      CAGGTTATTAATATTCAGTACTGCCGTTGCCAGCAGGCCACATGCGGTCGCCGGAAGGATCAGTGCCGGAATCAATGTATGAGCCTGTAAATAC                                         <  SRR4302533.6269524/94‑1 (MQ=60)

                                                       AGGTTATTAATATTCAGTACTGCCGTTGCCAGCAGGCCACATGCGGTCGCCGGAAGGATCAGTGCCGGAATCAATGTATGAGCCTGTAAATACC                                        >  SRR4302533.5672436/1‑94 (MQ=60)

                                                       AGGTTATTAATATTCAGTACTGCCGTTGCCAGCAGGCCACATGCGGTCGCCGGAAGGATCAGTGCCGGAATCAATGTATGAGCCTGTAAATACC                                        >  SRR4302533.1490940/1‑94 (MQ=60)

                                                       AGGTTATTAATATTCAGTACTGCCGTTGCCAGCAGGCCACATGCGGTCGCCGGAAGGATCAGTGCCGGAATCAATGTATGAGCCTGTAAATACC                                        >  SRR4302533.4228266/1‑94 (MQ=60)

                                                        GGTTATTAATATTCAGTACTGCCGTTGCCAGCAGGCCACATGCGGTCGCCGGAAGGATCAGTGCCGGAATCAATGTATGAGCCTGTAAATACCA                                       >  SRR4302533.4749900/1‑94 (MQ=60)

                                                         GTTATTAATATTCAGTACTGCCGTTGCCAGCAGGCCACATGCGGTCGCCGGAAGGATCAGTGCCGGAATCAATGTATGAGCCTGTAAATACCAG                                      >  SRR4302533.2426769/1‑94 (MQ=60)

                                                          TTATTAATATTCAGTACTGCCGTTGCCAGCAGGCCACATGCGGTCGCCGGAAGGATCAGTGCCGGAATCAATGTATGAGCCTGTAAATACCAGC                                     <  SRR4302533.157180/94‑1 (MQ=60)

                                                          TTATTAATATTCAGTACTGCCGTTGCCAGCAGGCCACATGCGGTCGCCGGAAGGATCAGTGCCGGAATCAATGTATGAGCCTGTAAATACCAGC                                     >  SRR4302533.207845/1‑94 (MQ=60)

                                                           TATTAATATTCAGTACTGCCGTTGCCAGCAGGCCACATGCGGTCGCCGGAAGGATCAGTGCCGGAATCAATGTATGAGCCTGTAAATACCAGCT                                    >  SRR4302533.3183436/1‑94 (MQ=60)

                                                           TATTAATATTCAGTACTGCCGTTGCCAGCAGGCCACATGCGGTCGCCGGAAGGATCAGTGCCGGAATCAATGTATGAGCCTGTAAATACCAGCT                                    >  SRR4302533.6306724/1‑94 (MQ=60)

                                                           TATTAATATTCAGTACTGCCGTTGCCAGCAGGCCACATGCGGTCGCCGGAAGGATCAGTGCCGGAATCAATGTATGAGCCTGTAAATACCAGCT                                    <  SRR4302533.4107886/94‑1 (MQ=60)

                                                             TTAATATTCAGTACTGCCGTTGCCAGCAGGCCACATGCGGTCGCCGGAAGGATCAGTGCCGGAATCAATGTATGAGCCTGTAAATACCAGCTCC                                  >  SRR4302533.3807869/1‑94 (MQ=60)

                                                             TTAATATTCAGTACTGCCGTTGCCAGCAGGCCACATGCGGTCGCCGGAAGGATCAGTGCCGGAATCAATGTATGAGCCTGTAAATACCAGCTCC                                  >  SRR4302533.682578/1‑94 (MQ=60)

                                                              TAATATTCAGTACTGCCGTTGCCAGCAGGCCACATGCGGTCGCCGGAAGGATCAGTGCCGGAATCAATGTATGAGCCTGTAAATACCAGCTCCC                                 >  SRR4302533.5941425/1‑94 (MQ=60)

                                                                ATATTCAGTACTGCCGTTGCCAGCAGGCCACATGCGGTCGCCGGAAGGATCAGTGCCGGAATCAATGTATGAGCCTGTAAATACCAGCTCCCCA                               >  SRR4302533.1158624/1‑94 (MQ=60)

                                                                 TATTCAGTACTGCCGTTGCCAGCAGGCCACATGCGGTCGCCGGAAGGATCAGTGCCGGAATCAATGTATGAGCCTGTAAATACCAGCTCCCCAT                              >  SRR4302533.1610780/1‑94 (MQ=60)

                                                                 TATTCAGTACTGCCGTTGCCAGCAGGCCACATGCGGTCGCCGGAAGGATCAGTGCCGGAATCAATGTATGAGCCTGTAAATACCAGCTCCCCAT                              <  SRR4302533.1672595/94‑1 (MQ=60)

                                                                 TATTCAGTACTGCCGTTGCCAGCAGGCCACATGCGGTCGCCGGAAGGATCAGTGCCGGAATCAATGTATGAGCCTGTAAATACCAGCTCCCCAT                              <  SRR4302533.5891675/94‑1 (MQ=60)

                                                                  ATTCAGTACTGCCGTTGCCAGCAGGCCACATGCGGTCGCCGGAAGGATCAGTGCCGGAATCAATGTATGAGCCTGTAAATACCAGCTCCCCATG                             >  SRR4302533.6195306/1‑94 (MQ=60)

                                                                  ATTCAGTACTGCCGTTGCCAGCAGGCCACATGCGGTCGCCGGAAGGATCAGTGCCGGAATCAATGTATGAGCCTGTAAATACCAGCTCCCCATG                             >  SRR4302533.2922479/1‑94 (MQ=60)

                                                                  ATTCAGTACTGCCGTTGCCAGCAGGCCACATGCGGTCGCCGGAAGGATCAGTGCCGGAATCAATGTATGAGCCTGTAAATACCAGCTCCCCATA                             >  SRR4302533.2105161/1‑94 (MQ=60)

                                                                    TCAGTACTGCCGTTGCCAGCAGGCCACATGCGGTCGCCGGAAGGATCAGTGCCGGAATCAATGTATGAGCCTGTAAATACCAGCTCCCCATGAC                           >  SRR4302533.5081405/1‑94 (MQ=60)

                                                                    TCAGTACTGCCGTTGCCAGCAGGCCACATGCGGTCGCCGGAAGGATCAGTGCCGGAATCAATGTATGAGCCTGTAAATACCAGCTCCCCATGAC                           <  SRR4302533.3108016/94‑1 (MQ=60)

                                                                     CAGTACTGCCGTTGCCAGCAGGCCACATGCGGTCGCCGGAAGGATCAGTGCCGGAATCAATTTATGAGCCTGTAAATACCAGCTCCCCATGACA                          >  SRR4302533.2446226/1‑94 (MQ=60)

                                                                     CAGTACTGCCGTTGCCAGCAGGCCACATGCGGTCGCCGGAAGGATCAGTGCCGGAATCAATGTATGAGCCTGTAAATACCAGCTCCCCATGACA                          >  SRR4302533.5538676/1‑94 (MQ=60)

                                                                     CAGTACTGCCGTTGCCAGCAGGCCACATGCGGTCGCCGGAAGGATCAGTGCCGGAATCAATGTATGAGCCTGTAAATACCAGCTCCCCATGACA                          <  SRR4302533.1044319/94‑1 (MQ=60)

                                                                     CAGTACTGCCGTTGCCAGCAGGCCACATGCGGTCGCCGGAAGGATCAGTGCCGGAATCAATGTATGAGCCTGTAAATACCAGCTCCCCATGACA                          >  SRR4302533.4535252/1‑94 (MQ=60)

                                                                      AGTACTGCCGTTGCCAGCAGGCCACATGCGGTCGCCGGAAGGATCAGTGCCGGAATCAATGTATGAGCCTGTAAATACCAGCTCCCCATGACAC                         <  SRR4302533.5882834/94‑1 (MQ=60)

                                                                      AGTACTGCCGTTGCCAGCAGGCCACATGCGGTCGCCGGAAGGATCAGTGCCGGAATCAATGTATGAGCCTGTAAATACCAGCTCCCCATGACAC                         >  SRR4302533.5763954/1‑94 (MQ=60)

                                                                       GTACTGCCGTTGCCAGCAGGCCACATGCGGTCGCCGGAAGGATCAGTGCCGGAATCAATGTATGAGCCTGTAAATACCAGCTCCCCATGACACT                        >  SRR4302533.6178465/1‑94 (MQ=60)

                                                                       GTACTGCCGTTGCCAGCAGGCCACATGCGGTCGCCGGAAGGATCAGTGCCGGAATCAATGTATGAGCCTGTAAATACCAGCTCCCCATGACACT                        <  SRR4302533.6237017/94‑1 (MQ=60)

                                                                         ACTGCCGTTGCCAGCAGGCCACATGCGGTCGCCGGAAGGATCAGTGCCGGAATCAATGTATGAGCCTGTAAATACCAGCTCCCCATGACACTCA                      >  SRR4302533.437975/1‑94 (MQ=60)

                                                                          CTGCCGTTGCCAGCAGGCCACATGCGGTCGCCGGAAGGATCAGTGCCGGAATCAATGTATGAGCCTGTAAATACCAGCTCCCCATGACACTCAA                     >  SRR4302533.5363758/1‑94 (MQ=60)

                                                                          CTGCCGTTGCCAGCAGGCCACATGCGGTCGCCGGAAGGATCAGTGCCGGAATCAATGTATGAGCCTGTAAATACCAGCTCCCCATGACACTCAA                     >  SRR4302533.525079/1‑94 (MQ=60)

                                                                           TGCCGTTGCCAGCAGGCCACATGCGGTCGCCGGAAGGATCAGTGCCGGAATCAATGTATGAGCCTGTAAATACCAGCTCCCCATGACACTCAAC                    <  SRR4302533.5674228/94‑1 (MQ=60)

                                                                           TGCCGTTGCCAGCAGGCCACATGCGGTCGCCGGAAGGATCAGTGCCGGAATCAATGTATGAGCCTGTAAATACCAGCTCCCCATGACACTCAAC                    <  SRR4302533.129045/94‑1 (MQ=60)

                                                                              CGTTGCCAGCAGGCCACATGCGGTCGCCGGAAGGATCAGTGCCGGAATCAATGTATGAGCCTGTAAATACCAGCTCCCCATGACACTCAACCAG                 <  SRR4302533.2638844/94‑1 (MQ=60)

                                                                               GTTGCCAGCAGGCCACATGCGGTCGCCGGAAGGATCAGTGCCGGAATCAATGTATGAGCCTGTAAATACCAGCTCCCCATGACACTCAACCAGC                >  SRR4302533.44501/1‑94 (MQ=60)

                                                                               GTTGCCAGCAGGCCACATGCGGTCGCCGGAAGGATCAGTGCCGGAATCAATGTATGAGCCTGTAAATACCAGCTCCCCATGACACTCAACCAGC                >  SRR4302533.4441002/1‑94 (MQ=60)

                                                                               GTTGCCAGCAGGCCACATGCGGTCGCCGGAAGGATCAGTGCCGGAATCAATGTATGAGCCTGTAAATACCAGCTCCCCATGACACTCAACCAGC                >  SRR4302533.4112540/1‑94 (MQ=60)

                                                                               GTTGCCAGCAGGCCACATGCGGTCGCCGGAAGGATCAGTGCCGGAATCAATGTATGAGCCTGTAAATACCAGCTCCCCATGACACTCAACCAGC                >  SRR4302533.1954864/1‑94 (MQ=60)

                                                                               GTTGCCAGCAGGCCACATGCGGTCGCCGGAAGGATCAGTGCCGGAATCAATGTATGAGCCTGTAAATACCAGCTCCCCATGACACTCAACCAGC                >  SRR4302533.6121888/1‑94 (MQ=60)

                                                                               GTTGCCAGCAGGCCACATGCGGTCGCCGGAAGGATCAGTGCCGGAATCAATGTATGAGCCTGTAAATACCAGCTCCCCATGACACTCAACCAGC                <  SRR4302533.3683383/94‑1 (MQ=60)

                                                                                   CCAGCAGGCCACATGCGGTCGCCGGAAGGATCAGTGCCGGAATCAATGTATGAGCCTGTAAATACCAGCTCCCCATGACACTCAACCAGCCAAA            >  SRR4302533.3125533/1‑94 (MQ=60)

                                                                                    CAGCAGGCCACATGCGGTTGCCGGAAGGATCAGTGCCGGAATCAATGGATGAGCCTGTAAATACCAGCTCCCCATGACACTCAACCAGCCAAAG           >  SRR4302533.4818827/1‑94 (MQ=60)

                                                                                    CAGCAGGCCACATGCGGTCGCCGGAAGGATCAGTGCCGGAATCAATGTATGAGCCTGTAAATACCAGCTCCCCATGACACTCAACCAGCCAAAG           >  SRR4302533.2323588/1‑94 (MQ=60)

                                                                                    CAGCAGGCCACATGCGGTCGCCGGAAGGATCAGTGCCGGAATCAATGTATGAGCCTGTAAATACCAGCTCCCCATGACACTCAACCAGCCAAAG           <  SRR4302533.4100057/94‑1 (MQ=60)

                                                                                    CAGCAGGCCACATGCGGTCGCCGGAAGGATCAGTGCCGGAATCAATGTATGAGCCTGTAAATACCAGCTCCCCATGACACTCAACCAGCCAAAG           >  SRR4302533.4427580/1‑94 (MQ=60)

                                                                                     AGCAGGCCACATGCGGTCGCCGGAAGGATCAGTGCCGGAATCAATGTATGAGCCTGTAAATACCAGCTCCCCATGACACTCAACCAGCCAAAGA          <  SRR4302533.3299635/94‑1 (MQ=60)

                                                                                     AGCAGGCCACATGCGGTCGCCGGAAGGATCAGTGCCGGAATCAATGTATGAGCCTGTAAATACCAGCTCCCCATGACACTCAACCAGCCAAAGA          >  SRR4302533.5945304/1‑94 (MQ=60)

                                                                                     AGCAGGCCACATGCGGTCGCCGGAAGGATCAGTGCCGGAATCAATGTATGAGCCTGTAAATACCAGCTCCCCATGACACTCAACCAGCCAAAGA          >  SRR4302533.5428377/1‑94 (MQ=60)

                                                                                       CAGGCCACATGCGGTCGCCGGAAGGATCAGTGCCGGAATCAATGTATGAGCCTGTAAATACCAGCTCCCCATGACACTCAACCAGCCAAAGAAA        <  SRR4302533.4892765/94‑1 (MQ=60)

                                                                                        AGGCCACATGCGGTCGCCGGAAGGATCAGTGCCGGAATCAATGTATGAGCCTGTAAATACCAGCTCCCCATGACACTCAACCAGCCAAAGAAAC       >  SRR4302533.2105039/1‑94 (MQ=60)

                                                                                         GGCCACATGCGGTCGCCGGAAGGATCAGTGCCGGAATCAATGTATGAGCCTGTAAATACCAGCTCCCCATGACACTCAACCAGCCAAAGAAAAC      >  SRR4302533.2635439/1‑94 (MQ=60)

                                                                                           CCACATGCGGTCGCCGGAAGGATCAGTGCCGGAATCAATGTATGAGCCTGTAAATACCAGCTCCCCATGACACTCAACCAGCCAAAGAAAACCA    <  SRR4302533.1121702/94‑1 (MQ=60)

                                                                                           CCACATGCGGTCGCCGGAAGGATCAGTGCCGGAATCAATGTATGAGCCTGTAAATACCAGCTCCCCATGACACTCAACCAGCCAAAGAAAACCA    >  SRR4302533.4268646/1‑94 (MQ=60)

                                                                                           CCACATGCGGTCGCCGGAAGGATCAGTGCCGGAATCAATGTATGAGCCTGTAAATACCAGCTCCCCATGACACTCAACCAGCCAAAGAAAACCA    >  SRR4302533.3582283/1‑94 (MQ=60)

                                                                                            CACATGCGGTCGCCGGAAGGATCAGTGCCGGAATCAATGTATGAGCCTGTAAATACCAGCTCCCCATGACACTCAACCAGCCAAAGAAAACCAG   <  SRR4302533.5086425/94‑1 (MQ=60)

                                                                                            CACATGCGGTCGCCGGAAGGATCAGTGCCGGAATCAATGTATGAGCCTGTAAATACCAGCTCCCCATGACACTCAACCAGCCAAAGAAAACCAG   <  SRR4302533.952126/94‑1 (MQ=60)

                                                                                             ACATGCGGTCGCCGGAAGGATCAGTGACGGAATCAATGTATGAGCCTGTAAATACCAGCTCCCCATGACACTCAACCAGCCAAAGAAAACCAGT  <  SRR4302533.337242/94‑1 (MQ=60)

TAAGCGCACCACCAGCGTGTTTTTGCCATTTTCGCGGTCGCTATTGATATCACGCAGGTTATTAATATTCAGTACTGCCGTTGCCAGCAGGCCGCATGCGGTCGCCGGAAGGATCAGTGCCGGAATCAATGTATGAGCCTGTAAATACCAGCTCCCCATGACACTCAACCAGCCAAAGAAAACCAGT  >  NC_000913/4117270‑4117456

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 17 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`