Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1624	250.6	12620048	98.0%	12367647	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,175,853	G→A	V178I (GTT→ATT)	nusG →	NusG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,175,853	0	G	A	100.0%	994.4 / NA	266	V178I (GTT→ATT)	nusG	NusG
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (126/140); total (126/140)

Only 100 of 267 total aligned reads displayed.

GTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTT  >  NC_000913/4175763‑4175942

    aagaagTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGaaa                                                                                     >  2:4522048/1‑93 (MQ=255)

     agaagTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGaaaa                                                                                    <  2:5028725/93‑1 (MQ=255)

      gaagTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGaaaaa                                                                                   <  1:6107609/93‑1 (MQ=255)

       aagTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAg                                                                                  <  1:5773325/93‑1 (MQ=255)

        agTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGc                                                                                 >  2:520823/1‑93 (MQ=255)

        agTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGc                                                                                 <  1:2225165/93‑1 (MQ=255)

          tGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCt                                                                               <  1:310797/93‑1 (MQ=255)

          tGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCt                                                                               >  1:3686937/1‑93 (MQ=255)

            gATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTaa                                                                             <  1:2500337/93‑1 (MQ=255)

            gATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTaa                                                                             >  1:3572741/1‑93 (MQ=255)

             aTTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAAc                                                                            <  2:1412166/93‑1 (MQ=255)

                aCGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCa                                                                         <  1:4282174/93‑1 (MQ=255)

                 cGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAg                                                                        >  2:484797/1‑93 (MQ=255)

                  gagaAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGc                                                                       <  1:4880689/93‑1 (MQ=255)

                  gagaAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGc                                                                       <  1:1527739/93‑1 (MQ=255)

                  gagaAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGc                                                                       >  2:4759752/1‑93 (MQ=255)

                  gagaAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGc                                                                       <  2:2761507/93‑1 (MQ=255)

                    gaAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGa                                                                     >  2:2673660/1‑93 (MQ=255)

                    gaAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGa                                                                     >  2:433416/1‑93 (MQ=255)

                    gaAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGa                                                                     <  1:1935915/93‑1 (MQ=255)

                      aaTCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTAGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATc                                                                   >  2:4109890/1‑93 (MQ=255)

                        tctcGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCaa                                                                 >  1:5871843/1‑93 (MQ=255)

                        tctcGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCaa                                                                 >  1:4940218/1‑93 (MQ=255)

                          tcGTCTGAGAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCaaaa                                                               <  1:5556588/93‑1 (MQ=255)

                          tcGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCaaaa                                                               >  2:3152922/1‑93 (MQ=255)

                           cggcTGAAATTGTCTGTTTCGATCTTCGGTGGTGCGACCCCGGTAGAGCTTGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCaaaaa                                                              <  1:5685402/90‑1 (MQ=255)

                            gTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCaaaaaa                                                             >  1:2330533/1‑93 (MQ=255)

                             tCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAg                                                            <  1:2182167/93‑1 (MQ=255)

                             gcTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAg                                                            <  1:1241622/92‑1 (MQ=255)

                               tGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAgcg                                                          >  1:5219955/1‑93 (MQ=255)

                                gAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAgcgg                                                         >  2:2013909/1‑93 (MQ=255)

                                 aaaGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAgcggc                                                        <  2:3484942/93‑1 (MQ=255)

                                 aaaGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAgcggc                                                        >  1:1323288/1‑93 (MQ=255)

                                  aaGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAgcggcg                                                       >  2:5770858/1‑93 (MQ=255)

                                   aGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGa                                                      <  2:6223061/93‑1 (MQ=255)

                                   aGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGa                                                      >  1:1581883/1‑93 (MQ=255)

                                    gtgtCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGAt                                                     >  2:1660281/1‑93 (MQ=255)

                                    gtgtCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGAt                                                     >  1:4071058/1‑93 (MQ=255)

                                    gtgtCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGAt                                                     <  1:4597398/93‑1 (MQ=255)

                                       tCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTa                                                  <  2:3558715/93‑1 (MQ=255)

                                       tCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTa                                                  >  2:5833309/1‑93 (MQ=255)

                                         tGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAAt                                                <  1:4146612/93‑1 (MQ=255)

                                         tGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAAt                                                <  2:11421/93‑1 (MQ=255)

                                         tGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAAt                                                >  2:4628219/1‑93 (MQ=255)

                                         tGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAAt                                                >  2:5411900/1‑93 (MQ=255)

                                          gTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATc                                               <  1:3278237/93‑1 (MQ=255)

                                          gTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATc                                               <  1:5171780/93‑1 (MQ=255)

                                          gTTTCTATCTTCGGTCGCGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATc                                               <  1:6226704/93‑1 (MQ=255)

                                           tttCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCg                                              >  1:841524/1‑93 (MQ=255)

                                           tttCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCg                                              <  1:5326638/93‑1 (MQ=255)

                                             tCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGtt                                            >  1:5506740/1‑93 (MQ=255)

                                              cTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAACAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTg                                           <  1:4436129/93‑1 (MQ=255)

                                              cTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTg                                           <  1:3462899/93‑1 (MQ=255)

                                                aTCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGca                                         >  1:3839474/1‑93 (MQ=255)

                                                  cTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGcaca                                       >  1:1749514/1‑93 (MQ=255)

                                                   ttCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACaa                                      <  1:2023960/93‑1 (MQ=255)

                                                   ttCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACaa                                      <  1:4669057/93‑1 (MQ=255)

                                                   ttCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACaa                                      <  1:1048828/93‑1 (MQ=255)

                                                   ttCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACaa                                      <  2:4415107/93‑1 (MQ=255)

                                                     cGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAgg                                    <  2:2579521/93‑1 (MQ=255)

                                                      ggTCGTGCGACCGCGGTAGAGCTGCACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGc                                   <  2:3242166/93‑1 (MQ=255)

                                                      ggTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGc                                   <  2:2951115/93‑1 (MQ=255)

                                                       gTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAcgcg                                  >  2:4157011/1‑93 (MQ=255)

                                                       gTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCg                                  >  2:5673589/1‑93 (MQ=255)

                                                        tCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGt                                 >  1:3313988/1‑93 (MQ=255)

                                                         cGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTg                                <  2:404134/93‑1 (MQ=255)

                                                         cGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTg                                >  1:2515145/1‑93 (MQ=255)

                                                         cGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTg                                <  2:2077096/93‑1 (MQ=255)

                                                          gTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTga                               >  2:1058653/1‑93 (MQ=255)

                                                           tGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTgag                              >  1:2243248/1‑93 (MQ=255)

                                                           tGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTgag                              >  2:2180405/1‑93 (MQ=255)

                                                           tGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTgag                              <  2:5954858/93‑1 (MQ=255)

                                                             cGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGAt                            <  2:3048285/93‑1 (MQ=255)

                                                              gACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGAtt                           <  2:5878982/93‑1 (MQ=255)

                                                               aCCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTg                          >  2:1517487/1‑93 (MQ=255)

                                                               aCCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTg                          >  2:1326182/1‑93 (MQ=255)

                                                               aCCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTg                          >  1:5958374/1‑93 (MQ=255)

                                                                  ccGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGATGCACAAGGCGTGAGAGTGGaa                       >  2:6308423/1‑93 (MQ=255)

                                                                   cGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAAt                      >  2:2336611/1‑93 (MQ=255)

                                                                   cGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAAt                      <  2:53624/93‑1 (MQ=255)

                                                                    ggTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATa                     <  2:4698670/93‑1 (MQ=255)

                                                                    ggTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATa                     >  2:1774865/1‑93 (MQ=255)

                                                                    ggTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATa                     >  2:823914/1‑93 (MQ=255)

                                                                    ggTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATa                     >  2:1360716/1‑93 (MQ=255)

                                                                     gTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATAc                    <  2:760992/93‑1 (MQ=255)

                                                                     gTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATAc                    <  2:2477136/93‑1 (MQ=255)

                                                                     gTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATAc                    >  2:2190834/1‑93 (MQ=255)

                                                                       agagCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACaa                  <  1:5663715/93‑1 (MQ=255)

                                                                        gagCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAAt                 <  1:4707160/93‑1 (MQ=255)

                                                                        gagCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAAt                 <  2:5487390/93‑1 (MQ=255)

                                                                         agCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGAACAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAAtt                <  2:3866277/93‑1 (MQ=255)

                                                                          gCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAAttt               <  2:3689721/93‑1 (MQ=255)

                                                                          gCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAAttt               >  2:6023480/1‑93 (MQ=255)

                                                                               aCTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCgcgc          <  2:1024547/93‑1 (MQ=255)

                                                                                 ttCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCt        <  2:3870932/93‑1 (MQ=255)

                                                                                  tCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCtt       >  1:4765026/1‑93 (MQ=255)

                                                                                   cAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCttt      <  2:888322/93‑1 (MQ=255)

                                                                                   cAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCttt      <  1:4365769/93‑1 (MQ=255)

                                                                                    aGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAATGCGTGAGATTGGAATACAATTTCGCGCCtttt     >  2:1557905/1‑93 (MQ=255)

                                                                                       cAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGtt  >  2:2794614/1‑93 (MQ=255)

GTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTT  >  NC_000913/4175763‑4175942

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

GTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTA  >  NC_000913/4175760‑4175946

GTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGT                                                                                               >  SRR4302533.4759975/1‑94 (MQ=60)

GTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGA                                                                                               >  SRR4302533.2014716/1‑94 (MQ=60)

 TTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGAT                                                                                              <  SRR4302533.962910/94‑1 (MQ=60)

 TTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGAT                                                                                              <  SRR4302533.92578/94‑1 (MQ=60)

 TTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGAT                                                                                              >  SRR4302533.2148353/1‑94 (MQ=60)

  TGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATT                                                                                             <  SRR4302533.1102704/94‑1 (MQ=60)

    TTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGA                                                                                           <  SRR4302533.3256593/94‑1 (MQ=60)

       AAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAA                                                                                        >  SRR4302533.3574464/1‑94 (MQ=60)

       AAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAA                                                                                        <  SRR4302533.4723209/94‑1 (MQ=60)

         GAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAG                                                                                      <  SRR4302533.4359653/94‑1 (MQ=60)

         GAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAG                                                                                      <  SRR4302533.2399280/94‑1 (MQ=60)

         GAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAG                                                                                      <  SRR4302533.6144484/94‑1 (MQ=60)

          AAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGC                                                                                     >  SRR4302533.2301898/1‑94 (MQ=60)

          AAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGC                                                                                     <  SRR4302533.5808192/94‑1 (MQ=60)

           AGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCC                                                                                    >  SRR4302533.2196154/1‑94 (MQ=60)

           AGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCC                                                                                    <  SRR4302533.2238904/94‑1 (MQ=60)

            GTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCT                                                                                   >  SRR4302533.618944/1‑94 (MQ=60)

            GTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCT                                                                                   >  SRR4302533.3709598/1‑94 (MQ=60)

            GTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCT                                                                                   <  SRR4302533.5564623/94‑1 (MQ=60)

             TGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTA                                                                                  <  SRR4302533.1757370/94‑1 (MQ=60)

             TGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTA                                                                                  <  SRR4302533.312700/94‑1 (MQ=60)

              GGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAA                                                                                 >  SRR4302533.3594754/1‑94 (MQ=60)

              GGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAA                                                                                 <  SRR4302533.1323796/94‑1 (MQ=60)

               GATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAAC                                                                                <  SRR4302533.2515760/94‑1 (MQ=60)

                ATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACC                                                                               <  SRR4302533.4328258/94‑1 (MQ=60)

                 TTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCC                                                                              >  SRR4302533.1353607/1‑94 (MQ=60)

                 TTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCC                                                                              <  SRR4302533.484846/94‑1 (MQ=60)

                  TACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCA                                                                             <  SRR4302533.1373037/94‑1 (MQ=60)

                   ACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAG                                                                            <  SRR4302533.4034519/94‑1 (MQ=60)

                   ACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAG                                                                            <  SRR4302533.4307911/94‑1 (MQ=60)

                    CGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCNGATTGAAAAAGCCTAACCCAGC                                                                           >  SRR4302533.5666388/1‑94 (MQ=60)

                    CGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGC                                                                           >  SRR4302533.2529400/1‑94 (MQ=60)

                    CGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGC                                                                           >  SRR4302533.3751122/1‑94 (MQ=60)

                     GAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCG                                                                          >  SRR4302533.2091078/1‑94 (MQ=60)

                     GAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCG                                                                          <  SRR4302533.1536932/94‑1 (MQ=60)

                     GAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCG                                                                          <  SRR4302533.4910257/94‑1 (MQ=60)

                       GAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGAT                                                                        <  SRR4302533.1947723/94‑1 (MQ=60)

                         AATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCA                                                                      <  SRR4302533.4939779/94‑1 (MQ=60)

                         AATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCA                                                                      <  SRR4302533.477207/94‑1 (MQ=60)

                          ATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAA                                                                     >  SRR4302533.5907337/1‑94 (MQ=60)

                          ATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAA                                                                     <  SRR4302533.1553964/94‑1 (MQ=60)

                          ATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAA                                                                     >  SRR4302533.4970126/1‑94 (MQ=60)

                           TCGCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAA                                                                    <  SRR4302533.2480464/94‑1 (MQ=60)

                             TCGTCTGAGAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAA                                                                  <  SRR4302533.5590168/94‑1 (MQ=60)

                              CGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAA                                                                 >  SRR4302533.2344963/1‑94 (MQ=60)

                              CGGCTGAAATTGTCTGTTTCGATCTTCGGTGGTGCGACCCCGGTAGAGCTTGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAA                                                                 <  SRR4302533.5719744/94‑1 (MQ=60)

                                TCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGC                                                               <  SRR4302533.2195619/94‑1 (MQ=60)

                                GCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGC                                                               <  SRR4302533.1249042/94‑1 (MQ=60)

                                 CTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCG                                                              >  SRR4302533.5251371/1‑94 (MQ=60)

                                 CTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCG                                                              <  SRR4302533.342285/94‑1 (MQ=60)

                                 CTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCG                                                              >  SRR4302533.5769138/1‑94 (MQ=60)

                                 CTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCG                                                              <  SRR4302533.3577824/94‑1 (MQ=60)

                                 CTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCAGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCG                                                              <  SRR4302533.1726753/94‑1 (MQ=60)

                                   TAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGC                                                            <  SRR4302533.2197099/94‑1 (MQ=60)

                                   GAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGC                                                            >  SRR4302533.1331226/1‑94 (MQ=60)

                                     AAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGA                                                          >  SRR4302533.5966943/1‑94 (MQ=60)

                                     AAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGA                                                          >  SRR4302533.1591411/1‑94 (MQ=60)

                                      AGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGAT                                                         >  SRR4302533.4818683/1‑94 (MQ=60)

                                      AGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGAT                                                         >  SRR4302533.4095840/1‑94 (MQ=60)

                                      AGTGTCTGTTNCTATCTTCGGTCGTGCGACCCCGGTAGAGCTNGACNTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGAT                                                         >  SRR4302533.4442926/1‑94 (MQ=60)

                                       GTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATT                                                        <  SRR4302533.4625249/94‑1 (MQ=60)

                                         GTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTA                                                      >  SRR4302533.2870974/1‑94 (MQ=60)

                                           CTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAAT                                                    >  SRR4302533.4103026/1‑94 (MQ=60)

                                            TGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATC                                                   >  SRR4302533.3187800/1‑94 (MQ=60)

                                            TGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATC                                                   <  SRR4302533.4171738/94‑1 (MQ=60)

                                            TGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATC                                                   >  SRR4302533.3612180/1‑94 (MQ=60)

                                             GTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCG                                                  <  SRR4302533.5202987/94‑1 (MQ=60)

                                             GTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCG                                                  <  SRR4302533.4271669/94‑1 (MQ=60)

                                             GTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCG                                                  <  SRR4302533.3298480/94‑1 (MQ=60)

                                             GTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCG                                                  >  SRR4302533.846442/1‑94 (MQ=60)

                                             GTTTCTATCTTCGGTCGCGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCG                                                  <  SRR4302533.6264007/94‑1 (MQ=60)

                                              TTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGT                                                 <  SRR4302533.5358560/94‑1 (MQ=60)

                                              TTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGT                                                 <  SRR4302533.4210165/94‑1 (MQ=60)

                                               TTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTT                                                <  SRR4302533.5831393/94‑1 (MQ=60)

                                               TTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTT                                                >  SRR4302533.5539970/1‑94 (MQ=60)

                                                TCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTG                                               <  SRR4302533.5481512/94‑1 (MQ=60)

                                                 CTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAACAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGC                                              <  SRR4302533.4462946/94‑1 (MQ=60)

                                                 CTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGC                                              <  SRR4302533.3484276/94‑1 (MQ=60)

                                                  TATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCA                                             >  SRR4302533.3862929/1‑94 (MQ=60)

                                                  CATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCA                                             <  SRR4302533.5243922/94‑1 (MQ=60)

                                                   ATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCAC                                            >  SRR4302533.4736227/1‑94 (MQ=60)

                                                    TCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACA                                           >  SRR4302533.1760167/1‑94 (MQ=60)

                                                     CTTCGGGCGTGCGGCCCCGGTAGAGCTTGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAA                                          <  SRR4302533.1106923/94‑1 (MQ=60)

                                                     CGTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAA                                          <  SRR4302533.2340627/94‑1 (MQ=60)

                                                      TTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAG                                         <  SRR4302533.2036457/94‑1 (MQ=60)

                                                      TTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAG                                         <  SRR4302533.4697350/94‑1 (MQ=60)

                                                      TTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAG                                         <  SRR4302533.1054995/94‑1 (MQ=60)

                                                      TTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAG                                         >  SRR4302533.5020127/1‑94 (MQ=60)

                                                      TTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAG                                         <  SRR4302533.1834412/94‑1 (MQ=60)

                                                       TCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGG                                        >  SRR4302533.1327027/1‑94 (MQ=60)

                                                        CGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGC                                       >  SRR4302533.4012580/1‑94 (MQ=60)

                                                        CGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGC                                       >  SRR4302533.2814701/1‑94 (MQ=60)

                                                         GGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCG                                      >  SRR4302533.196843/1‑94 (MQ=60)

                                                         GGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCG                                      >  SRR4302533.5228050/1‑94 (MQ=60)

                                                          GTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGT                                     >  SRR4302533.3334449/1‑94 (MQ=60)

                                                           TCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTG                                    >  SRR4302533.2530643/1‑94 (MQ=60)

                                                           TCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTG                                    <  SRR4302533.3317690/94‑1 (MQ=60)

                                                            CGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGA                                   <  SRR4302533.981606/94‑1 (MQ=60)

                                                            CGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGA                                   <  SRR4302533.5011122/94‑1 (MQ=60)

                                                             GTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAG                                  >  SRR4302533.2257114/1‑94 (MQ=60)

                                                             GTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAG                                  <  SRR4302533.5708077/94‑1 (MQ=60)

                                                               GCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGAT                                >  SRR4302533.3763259/1‑94 (MQ=60)

                                                               GCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGAT                                <  SRR4302533.5348184/94‑1 (MQ=60)

                                                               GCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGAT                                <  SRR4302533.4521279/94‑1 (MQ=60)

                                                                CGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATT                               <  SRR4302533.5559706/94‑1 (MQ=60)

                                                                 GACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTG                              >  SRR4302533.5994414/1‑94 (MQ=60)

                                                                 GACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTG                              <  SRR4302533.5791943/94‑1 (MQ=60)

                                                                  ACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGG                             <  SRR4302533.3375545/94‑1 (MQ=60)

                                                                    CCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAA                           >  SRR4302533.928676/1‑94 (MQ=60)

                                                                    CCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAA                           <  SRR4302533.4558029/94‑1 (MQ=60)

                                                                     CCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAAT                          >  SRR4302533.2245732/1‑94 (MQ=60)

                                                                     CCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAAT                          <  SRR4302533.61463/94‑1 (MQ=60)

                                                                     CCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAAT                          <  SRR4302533.5372937/94‑1 (MQ=60)

                                                                      CGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATA                         >  SRR4302533.1940924/1‑94 (MQ=60)

                                                                      CGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGACTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATA                         >  SRR4302533.758187/1‑94 (MQ=60)

                                                                       GGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATAC                        >  SRR4302533.2043836/1‑94 (MQ=60)

                                                                          AGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAAT                     <  SRR4302533.5697934/94‑1 (MQ=60)

                                                                           GAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATT                    <  SRR4302533.4735693/94‑1 (MQ=60)

                                                                            AGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTT                   >  SRR4302533.769457/1‑94 (MQ=60)

                                                                             GCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTC                  >  SRR4302533.4656589/1‑94 (MQ=60)

                                                                               TGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGC                >  SRR4302533.595939/1‑94 (MQ=60)

                                                                                   CTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCT            >  SRR4302533.5702516/1‑94 (MQ=60)

                                                                                   CTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCT            >  SRR4302533.606208/1‑94 (MQ=60)

                                                                                    TTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTT           >  SRR4302533.4793923/1‑94 (MQ=60)

                                                                                      CAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTT         <  SRR4302533.4392124/94‑1 (MQ=60)

                                                                                       AGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTG        >  SRR4302533.4677233/1‑94 (MQ=60)

                                                                                          CAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTT     >  SRR4302533.2851856/1‑94 (MQ=60)

                                                                                           AGATTGAAAAAGCCTAACCGAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTT    <  SRR4302533.2918506/94‑1 (MQ=60)

                                                                                           AGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTT    <  SRR4302533.2918505/94‑1 (MQ=60)

                                                                                            GATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTT   >  SRR4302533.3283280/1‑94 (MQ=60)

                                                                                            GATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTT   >  SRR4302533.345480/1‑94 (MQ=60)

                                                                                             ATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTA  >  SRR4302533.1858536/1‑94 (MQ=60)

                                                                                             ATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTA  >  SRR4302533.5907351/1‑94 (MQ=60)

GTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTA  >  NC_000913/4175760‑4175946

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 17 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`