Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,185,154 A→G M743V (ATG→GTG)  rpoC → RNA polymerase beta prime subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,185,1540AG100.0% 734.2 / NA 225M743V (ATG→GTG) rpoCRNA polymerase beta prime subunit
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (123/102);  total (123/102)

Only 100 of 233 total aligned reads displayed.

GAAGAGAAGCAGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTAC  >  NC_000913/4185064‑4185243
                                                                                          |                                                                                         
gAAGAGAAGCGGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGtgtg                                                                                         >  1:3169620/1‑93 (MQ=255)
 aaGAGAAGCGGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTGTGc                                                                                        >  1:5360424/1‑93 (MQ=255)
  agagAAGCGGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTGTGCg                                                                                       >  1:4885402/1‑93 (MQ=255)
   gagaAGCGGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTGTGCGt                                                                                      <  1:2016694/93‑1 (MQ=255)
     gaAGCGGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTGTGCGTgg                                                                                    <  2:49594/93‑1 (MQ=255)
       aGCGGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCGCAGATTCGTCAGCTTGCTGGTGTGCGTGGTc                                                                                  <  1:3809533/93‑1 (MQ=255)
        gcgggTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTGTGCGTGGTCt                                                                                 <  2:1610427/90‑1 (MQ=255)
        gcgggTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTGTGCGTGGTCt                                                                                 <  1:1851524/90‑1 (MQ=255)
         cgggTTTCCTTCAACAGCATCTACATGATGGCCGCCTCCGGTGCGCGTGGTTCTGCGGCACAGATTGGGCAGCTTGCTGGGGGGCGTGGTCTg                                                                                >  1:5952996/3‑93 (MQ=255)
         cgggTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTGTGCGTGGTCTg                                                                                <  1:5080626/91‑1 (MQ=255)
           ggTGTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGTTTCTGCGGCACAGATTCGTCAGCTTGCTGGTGTGCGTGGTCTGAt                                                                              <  1:1185673/93‑1 (MQ=255)
            gtgtCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTGTGCGTGGTCTGATg                                                                             <  2:541111/90‑1 (MQ=255)
            gTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTGTGCGTGGTCTGATg                                                                             >  1:859283/1‑93 (MQ=255)
            gTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTGTGCGTGGTCTGATg                                                                             >  2:6252790/1‑93 (MQ=255)
             tttCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTGTGCGTGGTCTGATgg                                                                            >  2:283519/1‑93 (MQ=255)
             tttCCTTCAACAGCATCTACATGATGGACGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTGTGCGTGGTCTGATgg                                                                            >  1:565467/1‑93 (MQ=255)
                ccTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTGTGCGTGGTCTGATGGCGa                                                                         >  1:4299224/1‑93 (MQ=255)
                 cTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTGTGCGTGGTCTGATGGCGaa                                                                        >  1:579967/1‑93 (MQ=255)
                  ttCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTGTGCGTGGTCTGATGGCGAAg                                                                       >  1:5303391/1‑93 (MQ=255)
                   tCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTGTGCGTGGTCTGATGGCGAAGc                                                                      <  1:2956320/93‑1 (MQ=255)
                   tCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTGTGCGTGGTCTGATGGCGAAGc                                                                      <  2:2139135/93‑1 (MQ=255)
                    cAACAGCATCTACATGATGGCCGACTCCGGGGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTGTGCGTGGTCTGATGGCGNAGcg                                                                     >  1:4754479/1‑92 (MQ=255)
                      aCAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTGTGCGTGGTCTGATGGCGAAGCCgg                                                                   >  1:2119279/1‑93 (MQ=255)
                       cAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTGTGCGTGGTCTGATGGCGAAGCCGGa                                                                  <  2:2548994/93‑1 (MQ=255)
                        aGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTGTGCGTGGTCTGATGGCGACGCCGGAt                                                                 >  1:5899198/1‑93 (MQ=255)
                        aGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTGTGCGTGGTCTGATGGCGAAGCCGGAt                                                                 <  2:280894/93‑1 (MQ=255)
                        aGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTGTGCGTGGTCTGATGGCGAAGCCGGAt                                                                 <  1:2634359/93‑1 (MQ=255)
                        aGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTGTGCGTGGTCTGATGGCGAAGCCGGAt                                                                 <  1:871676/93‑1 (MQ=255)
                         gCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTGTGCGTGGTCTGATGGCGAAGCCGGATg                                                                <  2:2642204/93‑1 (MQ=255)
                          cATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTGTGCGTGGTCTGATGGCGAAGCCGGATgg                                                               <  1:5458158/93‑1 (MQ=255)
                           aTCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTGTGCGTGGTCTGATGGCGCAGCCGGATGGc                                                              >  2:6142488/1‑93 (MQ=255)
                           aTCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTGTGCGTGGTCTGATGGCGAAGCCGGATgtc                                                              <  2:3125256/93‑3 (MQ=255)
                           aTCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTGTGCGTGGTCTGATGGCGAAGCCGGATGGc                                                              <  1:428309/93‑1 (MQ=255)
                             cTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTGTGCGTGGTCTGATGGCGAAGCCGGATGGCTc                                                            >  1:6215108/1‑93 (MQ=255)
                              tACATGATTGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTGTGCGTGGTCTGATGGCGAAGCCGGATGGCTcc                                                           >  2:5027947/1‑93 (MQ=255)
                              tACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTGTGCGTGGTCTGATGGCGAAGCCGGATGGCTcc                                                           >  1:6250457/1‑93 (MQ=255)
                               aCATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTGTGCGTGGTCTGATGGCGAAGCCGGATGGCTCca                                                          >  1:1532270/1‑93 (MQ=255)
                                cATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTGTGCGTGGTCTGATGGCGAAGCCGGATGGCTCcat                                                         >  1:3633245/1‑93 (MQ=255)
                                cATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTGTGCGTGGTCTGATGGCGAAGCCGGATGGCTCcat                                                         >  2:1198378/1‑93 (MQ=255)
                                cATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTGTGCGTGGTCTGATGGCGAAGCCGGATGGCTCcat                                                         >  1:2473638/1‑93 (MQ=255)
                                 atgatgGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTGTGCGTGGTCTGATGGCGAAGCCGGATGGCTCcatc                                                        <  2:5477316/93‑1 (MQ=255)
                                  tgatgGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTGTGCGTGGTCTGATGGCGAAGCCGGATGGCTCcatca                                                       >  1:2425088/1‑93 (MQ=255)
                                  tgatgGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTGTGCGTGGTCTGATGGCGAAGCCGGATGGCTCcatca                                                       >  2:5653363/1‑93 (MQ=255)
                                  tgatgGCCGACTCCGGCGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGGGTGCGTGGTCTGATGGCGAAGCCGGCTGGCTCcatca                                                       >  2:736812/1‑93 (MQ=255)
                                      ggCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTGTGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGa                                                   <  2:4995554/93‑1 (MQ=255)
                                        ccGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTGTGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGaaa                                                 <  2:770900/93‑1 (MQ=255)
                                         ctaCTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTGTGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAAc                                                <  2:598051/91‑1 (MQ=255)
                                          gACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTGTGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACg                                               >  1:3371975/1‑93 (MQ=255)
                                           gcTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTGTGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGc                                              <  1:1692588/92‑1 (MQ=255)
                                           aCTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTGTGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGc                                              >  2:3451156/1‑93 (MQ=255)
                                           aCTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTGTGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGc                                              >  2:633079/1‑93 (MQ=255)
                                           aCTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTGTGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGc                                              >  1:4365164/1‑93 (MQ=255)
                                            cTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTGTGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGcc                                             >  1:3484896/1‑93 (MQ=255)
                                            cTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTGTGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGcc                                             <  1:1286300/93‑1 (MQ=255)
                                             tCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTGTGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGTCa                                            >  2:2750845/1‑93 (MQ=255)
                                             tCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTGTGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCa                                            >  2:237935/1‑93 (MQ=255)
                                             tCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTGTGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCa                                            >  1:517527/1‑93 (MQ=255)
                                             tCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTGTGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCa                                            >  2:4771502/1‑93 (MQ=255)
                                              ccGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTGTGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCaa                                           >  2:4251494/1‑93 (MQ=255)
                                              ccGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTGTGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCaa                                           <  1:6160694/93‑1 (MQ=255)
                                               cGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTGTGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAAt                                          <  2:5098360/93‑1 (MQ=255)
                                               cGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTGTGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAAt                                          <  1:3090637/93‑1 (MQ=255)
                                               cGGTGCGCATGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTGTGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAAt                                          >  2:4413786/1‑93 (MQ=255)
                                                ggTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTGTGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATc                                         >  1:102302/1‑93 (MQ=255)
                                                 gTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTGTGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCa                                        >  2:1226556/1‑93 (MQ=255)
                                                   gcgcGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTGTGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCAcc                                      >  2:1904204/1‑93 (MQ=255)
                                                   gcgcGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTGTGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCAcc                                      >  2:2267698/1‑93 (MQ=255)
                                                   gcgcGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTGTGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCAcc                                      <  1:105232/93‑1 (MQ=255)
                                                     gcgTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTGTGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACcgc                                    >  2:3359938/1‑93 (MQ=255)
                                                        tGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTGTGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGaa                                 >  1:1663082/1‑93 (MQ=255)
                                                         ggTTCTGCGGCACAGATTCGTCAGCTTGCTGGTGTGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAAc                                <  1:688285/93‑1 (MQ=255)
                                                          gTTCTGCGGCACAGATTCGTCAGCTTGCTGGTGTGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACt                               <  2:1519333/93‑1 (MQ=255)
                                                           ttCTGCGGCACAGATTCGTCAGCTTGCTGGTGTGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACtt                              >  1:6286740/1‑93 (MQ=255)
                                                           ttCTGCGGCACAGATTCGTCAGCTTGCTGGTGTGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACtt                              <  1:3850257/93‑1 (MQ=255)
                                                           ttCTGCGGCACAGATTCGTCAGCTTGCTGGTGTGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACtt                              <  1:6100431/93‑1 (MQ=255)
                                                             cTGCGGCACAGATTCGTCAGCTTGCTGGTGTGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTcc                            <  2:1346004/93‑1 (MQ=255)
                                                               gCGGCACAGATTCGTCAGCTTGCTGGTGTGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGt                          >  2:1662620/1‑93 (MQ=255)
                                                                 ggCACAGATTCGTCAGCTTGCTGGTGTGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGa                        <  1:5756839/93‑1 (MQ=255)
                                                                  gCACAGATTCGTCAGCTTGCTGGTGTGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGaa                       <  2:2058488/93‑1 (MQ=255)
                                                                  gCACAGATTCGTCAGCTTGCTGGTGTGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGaa                       <  2:5897983/93‑1 (MQ=255)
                                                                  gCACAGATTCGTCAGCTTGCTGGTGTGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGaa                       <  2:5271698/93‑1 (MQ=255)
                                                                   cacaGATTCGTCAGCTTGCTGGTGTGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAg                      >  2:828599/1‑93 (MQ=255)
                                                                    acaGATTCGTCAGCTTGCTGGTGTGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAgg                     <  1:784153/93‑1 (MQ=255)
                                                                    acaGATTCGTCAGCTTGCTGGTGTGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAgg                     >  2:200725/1‑93 (MQ=255)
                                                                    acaGATTCGTCAGCTTGCTGGTGTGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAgg                     <  2:3516522/93‑1 (MQ=255)
                                                                     caGATTCGTCAGCTTGCTGGTGTGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGt                    <  2:4691403/93‑1 (MQ=255)
                                                                      aGATTCGTCAGCTTGCTGGTGTGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTc                   <  2:3709699/93‑1 (MQ=255)
                                                                         ttCGTCAGCTTGCTGGTGTGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGa                >  1:4716776/1‑93 (MQ=255)
                                                                         ttCGTCAGCTTGCTGGTGTGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGa                >  1:4024536/1‑93 (MQ=255)
                                                                             tCAGCTTGCTGGTGTGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGt            >  1:4666101/1‑93 (MQ=255)
                                                                              cAGCTTGCTGGTGTGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGCGAAGGTCTGAACGTa           >  1:4962800/1‑93 (MQ=255)
                                                                               aGCTTGCTGGTGTGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTAc          >  2:3982796/1‑93 (MQ=255)
                                                                               aGCTTGCTGGTGTGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTAc          >  1:1872331/1‑93 (MQ=255)
                                                                                gCTTGCTGGTGTGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACt         >  1:2742879/1‑93 (MQ=255)
                                                                                  ttGCTGGTGTGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTcc       <  1:465036/93‑1 (MQ=255)
                                                                                   tGCTGGTGTGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCa      <  1:4177256/93‑1 (MQ=255)
                                                                                      tGGTGTGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTa   >  2:2160828/1‑93 (MQ=255)
                                                                                       ggTGTGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTAc  >  1:6193845/1‑93 (MQ=255)
                                                                                       ggTGTGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTAc  <  1:2652631/93‑1 (MQ=255)
                                                                                       ggTGTGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTAc  <  2:430253/93‑1 (MQ=255)
                                                                                          |                                                                                         
GAAGAGAAGCAGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTAC  >  NC_000913/4185064‑4185243

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CAGGAAGAGAAGCAGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACT  >  NC_000913/4185061‑4185244
                                                                                             |                                                                                          
CAGGAAGAGAAGCGGGTTTCCTTCAACAGCATCTACATGATGGCCGCCTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTG                                                                                            <  SRR4302533.1118727/94‑1 (MQ=60)
CAGGAAGAGAAGCGGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTG                                                                                            >  SRR4302533.2578607/1‑94 (MQ=60)
 AGGAAGAGAAGCGGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTGT                                                                                           <  SRR4302533.4308171/94‑1 (MQ=60)
  GGAAGAGAAGCGGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTGTG                                                                                          >  SRR4302533.3189127/1‑94 (MQ=60)
   GAAGAGAAGCGGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTGTGC                                                                                         >  SRR4302533.5392616/1‑94 (MQ=60)
    AAGAGAAGCGGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTGTGCG                                                                                        >  SRR4302533.4914998/1‑94 (MQ=60)
     AGAGAAGCGGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTGTGCGT                                                                                       <  SRR4302533.3872319/94‑1 (MQ=60)
      GAGAAGCGGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTGTGCGTG                                                                                      <  SRR4302533.2029120/94‑1 (MQ=60)
       AGAAGCGGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTGTGCGTGG                                                                                     >  SRR4302533.127173/1‑94 (MQ=60)
          AGCGGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCGCAGATTCGTCAGCTTGCTGGTGTGCGTGGTCT                                                                                  <  SRR4302533.3832837/94‑1 (MQ=60)
           GCGGGTTTCCTTCAACAGCATCTACATGATGGCCGCCTCCGGTGCGCGTGGTTCTGCGGCACAGATTGGGCAGCTTGCTGGGGGGCGTGGTCTG                                                                                 >  SRR4302533.5989006/1‑94 (MQ=60)
           GCGGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTGTGCGTGGTCTG                                                                                 <  SRR4302533.1862812/94‑1 (MQ=60)
            CGGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTGTGCGTGGTCTGA                                                                                >  SRR4302533.6270331/1‑94 (MQ=60)
            CGGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTGTGCGTGGTCTGA                                                                                <  SRR4302533.5111332/94‑1 (MQ=60)
             GGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTGTGCGTGGTCTGAT                                                                               <  SRR4302533.6332395/94‑1 (MQ=60)
             GGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTGTGCGTGGTCTGAT                                                                               <  SRR4302533.3442291/94‑1 (MQ=60)
             GGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTGTGCGTGGTCTGAT                                                                               >  SRR4302533.5942867/1‑94 (MQ=60)
              GGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTGTGCGTGGTCTGATG                                                                              >  SRR4302533.864317/1‑94 (MQ=60)
              GGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTGTGCGTGGTCTGATG                                                                              <  SRR4302533.767769/94‑1 (MQ=60)
              GGTGTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGTTTCTGCGGCACAGATTCGTCAGCTTGCTGGTGTGCGTGGTCTGATG                                                                              <  SRR4302533.1192789/94‑1 (MQ=60)
               GTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTGTGCGTGGTCTGATGG                                                                             <  SRR4302533.1333767/94‑1 (MQ=60)
               GTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTGTGCGTGGTCTGATGG                                                                             <  SRR4302533.4383355/94‑1 (MQ=60)
               GTTTCCTTCAACAGCATCTACATGATGGACGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTGTGCGTGGTCTGATGG                                                                             >  SRR4302533.568819/1‑94 (MQ=60)
                TTTGCTTCAACAACATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTGTGCGTGGTCTGATGGC                                                                            <  SRR4302533.623066/94‑1 (MQ=60)
                TTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTGTGCGTGGTCTGATGGC                                                                            <  SRR4302533.3264621/94‑1 (MQ=60)
                  TCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTGTGCGTGGTCTGATGGCGA                                                                          >  SRR4302533.5818478/1‑94 (MQ=60)
                  TCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTGTGCGTGGTCTGATGGCGA                                                                          >  SRR4302533.4325080/1‑94 (MQ=60)
                   CCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTGTGCGTGGTCTGATGGCGAA                                                                         >  SRR4302533.583382/1‑94 (MQ=60)
                    CTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTGTGCGTGGTCTGATGGCGAAG                                                                        <  SRR4302533.5778679/94‑1 (MQ=60)
                    CTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTGTGCGTGGTCTGATGGCGAAG                                                                        >  SRR4302533.5335163/1‑94 (MQ=60)
                    CTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTGTGCGTGGTCTGATGGCGAAG                                                                        >  SRR4302533.5915041/1‑94 (MQ=60)
                    CTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTGTGCGTGGTCTGATGGCGAAG                                                                        >  SRR4302533.6106291/1‑94 (MQ=60)
                    CTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTGTGCGTGGTCTGATGGCGAAG                                                                        <  SRR4302533.1865451/94‑1 (MQ=60)
                      TCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTGTGCGTGGTCTGATGGCGAAGCC                                                                      <  SRR4302533.2974393/94‑1 (MQ=60)
                      TCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTGTGCGTGGTCTGATGGCGAAGCC                                                                      >  SRR4302533.4965296/1‑94 (MQ=60)
                      TCAACAGCATCTACATGATGGCCGACTCCGGGGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTGTGCGTGGTCTGATGGCGNAGCG                                                                      >  SRR4302533.4783328/1‑94 (MQ=60)
                        AACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTGTGCGTGGTCTGATGGCGAAGCCGG                                                                    >  SRR4302533.2132351/1‑94 (MQ=60)
                          CAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTGTGCGTGGTCTGATGGCGACGCCGGAT                                                                  >  SRR4302533.5934825/1‑94 (MQ=60)
                          CAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTGTGCGTGGTCTGATGGCGAAGCCGGAT                                                                  <  SRR4302533.3369926/94‑1 (MQ=60)
                          CAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTGTGCGTGGTCTGATGGCGAAGCCGGAT                                                                  <  SRR4302533.4897176/94‑1 (MQ=60)
                          CAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTGTGCGTGGTCTGATGGCGAAGCCGGAT                                                                  <  SRR4302533.2971414/94‑1 (MQ=60)
                          CAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTGTGCGTGGTCTGATGGCGAAGCCGGAT                                                                  <  SRR4302533.5837021/94‑1 (MQ=60)
                           AGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTGTGCGTGGTCTGATGGCGAAGCCGGATG                                                                 <  SRR4302533.5227744/94‑1 (MQ=60)
                           AGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTGTGCGTGGTCTGATGGCGAAGCCGGATG                                                                 <  SRR4302533.876793/94‑1 (MQ=60)
                           AGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTGTGCGTGGTCTGATGGCGAAGCCGGATG                                                                 <  SRR4302533.2650548/94‑1 (MQ=60)
                           AGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTGTGCGTGGTCTGATGGCGAAGCCGGATG                                                                 >  SRR4302533.2059736/1‑94 (MQ=60)
                             CATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTGTGCGTGGTCTGATGGCGAAGCCGGATGGC                                                               <  SRR4302533.5491040/94‑1 (MQ=60)
                              ATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTGTGCGTGGTCTGATGGCGAAGCCGGATGGCT                                                              <  SRR4302533.430863/94‑1 (MQ=60)
                              ATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTGTGCGTGGTCTGATGGCGAAGCCGGATGGCT                                                              >  SRR4302533.6174634/1‑94 (MQ=60)
                               TCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTGTGCGTGGTCTGATGGCGAAGCCGGATGGCTC                                                             >  SRR4302533.6252374/1‑94 (MQ=60)
                                CTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTGTGCGTGGTCTGATGGCGAAGCCGGATGGCTCC                                                            >  SRR4302533.546321/1‑94 (MQ=60)
                                CTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTGTGCGTGGTCTGATGGCGAAGCCGGATGGCTCC                                                            >  SRR4302533.6287851/1‑94 (MQ=60)
                                 TACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTGTGCGTGGTCTGATGGCGAAGCCGGATGGCTCCA                                                           >  SRR4302533.1541475/1‑94 (MQ=60)
                                  ACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTGTGCGTGGTCTGATGGCGAAGCCGGATGGCTCCAT                                                          >  SRR4302533.2488891/1‑94 (MQ=60)
                                  ACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTGTGCGTGGTCTGATGGCGAAGCCGGATGGCTCCAT                                                          >  SRR4302533.3655595/1‑94 (MQ=60)
                                   CATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTGTGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATC                                                         >  SRR4302533.47574/1‑94 (MQ=60)
                                    ATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTGTGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCA                                                        >  SRR4302533.2440059/1‑94 (MQ=60)
                                    ATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTGTGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCA                                                        >  SRR4302533.2661814/1‑94 (MQ=60)
                                     TGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTGTGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCAT                                                       <  SRR4302533.2339653/94‑1 (MQ=60)
                                      GATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTGTGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATC                                                      >  SRR4302533.2771825/1‑94 (MQ=60)
                                       ATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTGTGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCG                                                     >  SRR4302533.6274737/1‑94 (MQ=60)
                                       ATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTGTGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCG                                                     >  SRR4302533.4003450/1‑94 (MQ=60)
                                       ATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTGTGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCG                                                     >  SRR4302533.2588253/1‑94 (MQ=60)
                                         GGCCGACTCCGGTGCGCGTGGTTCTGCGTCACAGATTCGTCAGCTTGCTGGTGTGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAA                                                   <  SRR4302533.4315669/94‑1 (MQ=60)
                                         GGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTGTGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAA                                                   <  SRR4302533.5375528/94‑1 (MQ=60)
                                           CCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTGTGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAAC                                                 >  SRR4302533.3009557/1‑94 (MQ=60)
                                           CCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTGTGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAAC                                                 <  SRR4302533.184774/94‑1 (MQ=60)
                                            CGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTGTGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACG                                                >  SRR4302533.3392797/1‑94 (MQ=60)
                                            CGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTGTGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACG                                                <  SRR4302533.3804355/94‑1 (MQ=60)
                                            CGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTGTGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACG                                                >  SRR4302533.1833032/1‑94 (MQ=60)
                                            CGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTGTGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACG                                                >  SRR4302533.5529432/1‑94 (MQ=60)
                                             GACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTGTGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGC                                               >  SRR4302533.1702331/1‑94 (MQ=60)
                                             GACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTGTGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGC                                               >  SRR4302533.4391518/1‑94 (MQ=60)
                                             GACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTGTGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGC                                               >  SRR4302533.4194405/1‑94 (MQ=60)
                                              GCTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTGTGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCC                                              <  SRR4302533.1702841/94‑1 (MQ=60)
                                              ACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTGTGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCC                                              >  SRR4302533.3506387/1‑94 (MQ=60)
                                               CTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTGTGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCA                                             >  SRR4302533.520603/1‑94 (MQ=60)
                                               CTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTGTGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCA                                             >  SRR4302533.2452147/1‑94 (MQ=60)
                                               CTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTGTGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCA                                             <  SRR4302533.1294039/94‑1 (MQ=60)
                                                TCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTGTGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAA                                            >  SRR4302533.4745125/1‑94 (MQ=60)
                                                TCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTGTGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAA                                            >  SRR4302533.2536770/1‑94 (MQ=60)
                                                 CCGGTGCGCGTGGTTCTGCGGCACAGCTTCGTCAGCTTGCTGGTGTGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAAT                                           >  SRR4302533.837976/1‑94 (MQ=60)
                                                 CCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTGTGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAAT                                           <  SRR4302533.6197743/94‑1 (MQ=60)
                                                 CCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTGTGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAAT                                           <  SRR4302533.1447310/94‑1 (MQ=60)
                                                 CCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTGTGAGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAAT                                           >  SRR4302533.1560368/1‑94 (MQ=60)
                                                  CGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTGTGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATC                                          >  SRR4302533.102940/1‑94 (MQ=60)
                                                  CGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTGTGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATC                                          <  SRR4302533.3109638/94‑1 (MQ=60)
                                                      GCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTGTGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCG                                      <  SRR4302533.105886/94‑1 (MQ=60)
                                                       CGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTGTGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGC                                     >  SRR4302533.1045890/1‑94 (MQ=60)
                                                        GCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTGTGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCG                                    >  SRR4302533.869034/1‑94 (MQ=60)
                                                          GTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTGTGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAA                                  >  SRR4302533.1673134/1‑94 (MQ=60)
                                                          GTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTGTGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAA                                  >  SRR4302533.1061377/1‑94 (MQ=60)
                                                            GGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTGTGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACT                                <  SRR4302533.692355/94‑1 (MQ=60)
                                                             GTTCTGCGGCACAGATTCGTCAGCTTGCTGGTGTGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTT                               >  SRR4302533.6324247/1‑94 (MQ=60)
                                                              TTCTGCGGCACAGATTCGTCAGCTTGCTGGTGTGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTC                              <  SRR4302533.6137268/94‑1 (MQ=60)
                                                              TTCTGCGGCACAGATTCGTCAGCTTGCTGGTGTGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTC                              <  SRR4302533.3873768/94‑1 (MQ=60)
                                                                    GGCACAGATTCGTCAGCTTGCTGGTGTGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAA                        <  SRR4302533.5791578/94‑1 (MQ=60)
                                                                    GGCACAGATTCGTCAGCTTGCTGGTGTGCGGGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAAAGCCAATCCCCGCGAACTTCCGTtag                        >  SRR4302533.4683500/1‑91 (MQ=60)
                                                                     GCACAGATTCGTCAGCTTGCTGGTGTGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAG                       <  SRR4302533.702440/94‑1 (MQ=60)
                                                                     GCACAGATTCGTCAGCTTGCTGGTGTGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAG                       >  SRR4302533.5497633/1‑94 (MQ=60)
                                                                     GCACAGATTCGTCAGCTTGCTGGTGTGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAG                       <  SRR4302533.2719949/94‑1 (MQ=60)
                                                                      CACAGATTCGTCAGCTTGCTGGTGTGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGG                      >  SRR4302533.4915611/1‑94 (MQ=60)
                                                                       ACAGATTCGTCAGCTTGCTGGTGTGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGT                     <  SRR4302533.788766/94‑1 (MQ=60)
                                                                           ATTCGTCAGCTTGCTGGTGTGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAACAGCCAATCACCGCGAACTTCCGTGAAGGGCTGA                 >  SRR4302533.4745373/1‑94 (MQ=60)
                                                                           ATTCGTCAGCTTGCTGGTGTGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGA                 >  SRR4302533.4745374/1‑94 (MQ=60)
                                                                           ATTCGTCAGCTTGCTGGTGTGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGA                 >  SRR4302533.4049036/1‑94 (MQ=60)
                                                                             TCGTCAGCTTGCTGGTGTGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAAC               <  SRR4302533.6290191/94‑1 (MQ=60)
                                                                             TCGTCAGCTTGCTGGTGTGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTAAAGGTCTGAAC               <  SRR4302533.6153279/94‑1 (MQ=60)
                                                                               GTCAGCTTGCTGGTGTGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGT             >  SRR4302533.4694371/1‑94 (MQ=60)
                                                                                TCAGCTTGCTGGTGTGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTA            <  SRR4302533.3178416/94‑1 (MQ=60)
                                                                                TCAGCTTGCTGGTGTGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTA            >  SRR4302533.4348211/1‑94 (MQ=60)
                                                                                TCAGCTTGCTGGTGTGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGCGAAGGTCTGAACGTA            >  SRR4302533.4992889/1‑94 (MQ=60)
                                                                                 CAGCTTGCTGGTGTGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTAC           >  SRR4302533.1883746/1‑94 (MQ=60)
                                                                                 CAGCTTGCTGGTGTGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTAC           >  SRR4302533.3826592/1‑94 (MQ=60)
                                                                                  AGCTTGCTGGTGTGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACT          >  SRR4302533.4402498/1‑94 (MQ=60)
                                                                                  AGCTTGCTGGTGTGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACT          >  SRR4302533.2759696/1‑94 (MQ=60)
                                                                                  AGCTTGCTGGTGTGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTAAT          >  SRR4302533.5275039/1‑94 (MQ=60)
                                                                                   GCTTGCTGGTGTGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTC         <  SRR4302533.5141970/94‑1 (MQ=60)
                                                                                    CTTGCTGGTGTGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCC        <  SRR4302533.1997490/94‑1 (MQ=60)
                                                                                     TTGCTGGTGTGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCA       <  SRR4302533.467811/94‑1 (MQ=60)
                                                                                      TGCTGGTGTGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAG      <  SRR4302533.4214203/94‑1 (MQ=60)
                                                                                      TGCTGGTGTGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAG      <  SRR4302533.4202564/94‑1 (MQ=60)
                                                                                       GCTGGTGTGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGT     <  SRR4302533.4055531/94‑1 (MQ=60)
                                                                                        CTGGTGTGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCCCCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTA    <  SRR4302533.6209637/94‑1 (MQ=60)
                                                                                        CTGGTGTGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTA    <  SRR4302533.3573838/94‑1 (MQ=60)
                                                                                        CTGGTGTGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTA    <  SRR4302533.2095899/94‑1 (MQ=60)
                                                                                        CTGGTGTGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTA    >  SRR4302533.5017209/1‑94 (MQ=60)
                                                                                         TGNNNNNCNTNNTNTGATGNNNANNCCNGANGNCTCCATNANNGAAACGCAAATCANCNCGACCTTCCGTNNAGGTCTGAACGTACTCCAGTAC   <  SRR4302533.836398/94‑1 (MQ=34)
                                                                                         TGGTGTGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTAC   >  SRR4302533.6231010/1‑94 (MQ=60)
                                                                                          GGTGTGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACT  <  SRR4302533.2668927/94‑1 (MQ=60)
                                                                                             |                                                                                          
CAGGAAGAGAAGCAGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACT  >  NC_000913/4185061‑4185244

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑