Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1624	250.6	12620048	98.0%	12367647	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,246,452	T→C	G301G (GGT→GGC)	lamB →	phage lambda receptor protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,246,452	0	T	C	99.7%	947.5 / NA	294	G301G (GGT→GGC)	lamB	phage lambda receptor protein
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (146/147); minor base A (0/1); total (146/148)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 308 total aligned reads displayed.

GTCGCAGGGTTCTGGCGTTGCATTTGATAACGAAAAATTTGCCTACAATATCAACAACAACGGTCACATGCTGCGTATCCTCGACCACGGTGCGATCTCCATGGGCGACAACTGGGACATGATGTACGTGGGTATGTACCAGGATATCAACTGGGATAACGACAACGGCACCAAGTGGTG  >  NC_000913/4246362‑4246541

gTCGCAGGGTTCTGGCGTTGCATTTGATAACGAAAAATTTGCCTACAATATCAACAACAACGGTCACATGCTGCGTATCCTCGACCACGgcgc                                                                                         <  1:1041498/93‑1 (MQ=255)

gTCGCAGGGTTCTGGCGTTGCATTTGATAACGAAAAATTTGCCTACAATATCAACAACAACGGTCACATGCTGCGTATCCTCGACCACGgcgc                                                                                         <  1:458705/93‑1 (MQ=255)

gTCGCAGGGTTCTGGCGTTGCATTTGATAACGAAAAATTTGCCTACAATATCAACAACAACGGTCACATGCTGCGTATCCTCGACCACGgcgc                                                                                         <  1:5483761/93‑1 (MQ=255)

 tCGCAGGGTTCTGGCGTTGCATTTGATAACGAAAAATTTGCCTACAATATCAACAACAACGGTCACATGCTGCGTATCCTCGACCACGGcgcg                                                                                        >  2:738569/1‑93 (MQ=255)

   gCAGGGTTCTGGCGTTGCATTTGATAACGAAAAATTTGCCTACAATATCAACAACAACGGTCACATGCTGCGTATCCTCGACCACGGCGCGAt                                                                                      >  2:5595967/1‑93 (MQ=255)

    cAGGTTTCTGGCGTTGCATTTGATAACGAAAAATTTGCCTACAATATCAACAACAACGGTCACATGCTGCGTATCCTCGACCACGGCGCGAtc                                                                                     <  1:2336546/93‑1 (MQ=255)

     aGGGTTCTGGCGTTGCATTTGATAACGAAAAATTTGCCTACAATATCAACAACAACGGTCACATGCTGCGTATCCTCGACCACGGCGCGAtct                                                                                    >  1:3854913/1‑93 (MQ=255)

      gggTTCTGGCGTTGCATTTGATAACGAAAAATTTGCCTACAATATCAACAACAACGGTCACATGCTGCGTATCCTCGACCACGGCGCGAtctc                                                                                   <  1:2224608/93‑1 (MQ=255)

      gggTTCTGGCGTTGCATTTGATAACGAAAAATTTGCCTACAATATCAACAACAACGGTCACATGCTGCGTATCCTCGACCACGGCGCGAtctc                                                                                   >  2:2782450/1‑93 (MQ=255)

      gggTTCTGGCGTTGCATTTGATAACGAAAAATTTGCCTACAATATCAACAACAACGGTCACATGCTGCGTATCCTCGACCACGGCGCGAtctc                                                                                   >  2:2954351/1‑93 (MQ=255)

        gTTCTGGCGTTGCATTTGATAACGAAAAATTTGCCTACAATATCAACAACAACGGTCACATGCTGCGTATCCTCGACCACGGCGCGATCTCCa                                                                                 >  2:5712605/1‑93 (MQ=255)

          tCTGGCGTTGCATTTGATAACGAAAAATTTGCCTACAATATCAACAACAACGGTCACATGCTGCGTATCCTCGACCACGGCGCGATCTCCATg                                                                               <  1:962145/93‑1 (MQ=255)

            tGGCGTTGCATTTGATAACGAAAAATTTGCCTACAATATCAACAACAACGGTCACATGCTGCGTATCCTCGACCACGGCGCGATCTCCATggg                                                                             <  1:6093448/93‑1 (MQ=255)

             ggCGTTGCATTTGATAACGAAAAATTTGCCTACAATATCAACAACAACGGTCACATGCTGCGTATCCTCGACCACGGCGCGATCTCCATGGGc                                                                            <  1:3529509/93‑1 (MQ=255)

               cGTTGCATTTGATAACGAAAAATTTGCCTACAATATCAACAACAACGGTCACATGCTGCGTATCCTCGACCACGGCGCGATCTCCATGGGCGa                                                                          >  1:3443976/1‑93 (MQ=255)

                gTTGCATTTGATAACGAAAAATTTGCCTACAATATCAACAACAACGGTCACATGCTGCGTATCCTCGACCACGGCGCGATCTCCATGGGCGAc                                                                         >  1:1539252/1‑93 (MQ=255)

                gTTGCATTTGATAACGAAAAATTTGCCTACAATATCAACAACAACGGTCACATGCTGCGTATCCTCGACCACGGCGCGATCTCCATGGGCGAc                                                                         >  1:2978904/1‑93 (MQ=255)

                 ttGCATTTGATAACGAAAAATTTGCCTACAATATCAACAACAACGGTCACATGCTGCGTATCCTCGACCACGGCGCGATCTCCATGGGCGACa                                                                        >  2:4117073/1‑93 (MQ=255)

                  tGCATTTGATAACGAAAAATTTGCCTACAATATCAACAACAACGGTCACATGCTGCGTATCCTCGACCACGGCGCGATCTCCATGGGCGACaa                                                                       >  1:1931266/1‑93 (MQ=255)

                  tGCATTTGATAACGAAAAATTTGCCTACAATATCAACAACAACGGTCACATGCTGCGTATCCTCGACCACGGCGCGATCTCCATGGGCGACaa                                                                       <  2:5328581/93‑1 (MQ=255)

                   gCATTTGATAACGAAAAATTTGCCTACAATATCAACAACAACGGTCACATGCTGCGTATCCTCGACCACGGCGCGATCTCCATGGGCGACAAc                                                                      <  2:3589656/93‑1 (MQ=255)

                   gCATTTGATAACGAAAAATTTGCCTACAATATCAACAACAACGGTCACATGCTGCGTATCCTCGACCACGGCGCGATCTCCATGGGCGACAAc                                                                      <  1:1236238/93‑1 (MQ=255)

                   gCATTTGATAACGAAAAATTTGCCTACAATATCAACAACAACGGTCACATGCTGCGTATCCTCGACCACGGCGCGATCTCCATGGGCGACAAc                                                                      >  1:2816442/1‑93 (MQ=255)

                   gCATTTGATAACGAAAAATGTGCCTACAATATCAACAACAACGGTCACATGCTGCGTATCCTCGACCACGGCGCGATCTCCATGGGCGACAAc                                                                      <  1:262920/93‑1 (MQ=255)

                    cATTTGATAACGAAAAATTTGCCTACAATATCAACAACAACGGTCACATGCTGCGTATCCTCGACCACGGCGCGATCTCCATGGGCGACAACt                                                                     >  2:6272094/1‑93 (MQ=255)

                    cATTTGATAACGAAAAATTTGCCTACAATATCAACAACAACGGTCACATGCTGCGTATCCTCGACCACGGCGCGATCTCCATGGGCGACAACt                                                                     >  1:748678/1‑93 (MQ=255)

                     aTTTGATAACGAAAAATTTGCCTACAATATCAACAACAACGGTCACATGCTGCGTATCCTCGACCACGGCGCGATCTCCATGGGCGACAACTg                                                                    >  1:132663/1‑93 (MQ=255)

                     aTTTGATAACGAAAAATTTGCCTACAATATCAACAACAACGGTCACATGCTGCGTATCCTCGACCACGGCGCGATCTCCATGGGCGACAACTg                                                                    >  1:3607853/1‑93 (MQ=255)

                     aTTTGATAACGAAAAATTTGCCTACAATATCAACAACAACGGTCACATGCTGCGTATCCTCGACCACGGCGCGATCTCCATGGGCGACAACTg                                                                    <  2:5295012/93‑1 (MQ=255)

                      tttGATAACGAAAAATTTGCCTACAATATCAACAACAACGGTCACATGCTGCGTATCCTCGACCACGGCGCGATCTCCATGGGCGACAACTgg                                                                   >  1:624890/1‑93 (MQ=255)

                      tttGATAACGAAAAATTTGCCTACAATATCAACAACAACGGTCACATGCTGCGTATCCTCGACCACGGCGCGATCTCCATGGGCGACAACTgg                                                                   >  1:2945589/1‑93 (MQ=255)

                        tGATAACGAAAAATTTGCCTACAATATCAACAACAACGGTCACATGCTGCGTATCCTCGACCACGGCGCGATCTCCATGGGCGACAACTGGGa                                                                 <  2:1206350/93‑1 (MQ=255)

                        tGATAACGAAAAATTTGCCTACAATATCAACAACAACGGTCACATGCTGCGTATCCTCGACCACGGCGCGATCTCCATGGGCGACAACTGGGa                                                                 <  1:5531843/93‑1 (MQ=255)

                         gATAACGAAAAATTTGCCTACAATATCAACAACAACGGTCACATGCTGCGTATCCTCGACCACGGCGCGATCTCCATGGGCGACAACTGGGAc                                                                >  1:5116459/1‑93 (MQ=255)

                         gATAACGAAAAATTTGCCTACAATATCAACAACAACGGTCACATGCTGCGTATCCTCGACCACGGCGCGATCTCCATGGGCGACAACTGGGAc                                                                <  1:5994806/93‑1 (MQ=255)

                          aTAACGAAAAATTTGCCTACAATATCAACAACAACGGTCACATGCTGCGTATCCTCGACCACGGCGCGATCTCCATGGGCGACAACTGGGACa                                                               <  2:2271396/93‑1 (MQ=255)

                           tAACGAAAAATTTGCCTACAATATCAACAACAACGGTCACATGCTGCGTATCCTCGACCACGGCGCGATCTCCATGGGCGACAACTGGGACat                                                              >  1:4094494/1‑93 (MQ=255)

                            aaCGAAAAATTTGCCTACAATATCAACAACAACGGTCACATGCTGCGTATCCTCGACCACGGCGCGATCTCCATGGGCGACAACTGGGACatg                                                             >  2:3974185/1‑93 (MQ=255)

                             aCGAAAAATTTGCCTACAATATCAACAACAACGGTCACATGCTGCGTATCCTCGACCACGGCGCGATCTCCATGGGCGACAACTGGGACatga                                                            <  2:1718771/93‑1 (MQ=255)

                               gAAAAATTTGCCTACAATATCAACAACAACGGTCACATGCTGCGTATCCTCGACCACGGCGCGATCTCCATGCGCGACAACTGGGACatgatg                                                          >  2:3273448/1‑93 (MQ=255)

                                aaaaaTTTGCCTACAATATCAACAACAACGGTCACATGCTGCGTATCCTCGACCACGGCGCGATCTCCATGGGCGACAACTGGGACATGATGt                                                         >  2:4544615/1‑93 (MQ=255)

                                  aaaTTTGCCTACAATATCAACAACAACGGTCACATGCTGCGTATCCTCGACCACGGCGCGATCTCCATGGGCGGCAACTGGGACATGATGTAc                                                       <  2:6148410/93‑1 (MQ=255)

                                  aaaTTTGCCTACAATATCAACAACAACGGTCACATGCTGCGTATCCTCGACCACGGCGCGATCTCCATGGGCGACAACTGGGACATGATGTAc                                                       <  2:1349078/93‑1 (MQ=255)

                                    aTTTGCCTACAATATCAACAACAACGGTCACATGCTGCGTATCCTCGACCACGGCGCGATCTCCATGGGCGACAACTGGGACATGATGTACgg                                                     >  1:4927647/1‑92 (MQ=255)

                                    aTTTGCCTACAATATCAACAACAACGGTCACATGCTGCGTATCCTCGACCACGGCGCGATCTCCATGGGCGACAACTGGGACATGATGTACGt                                                     >  2:37475/1‑93 (MQ=255)

                                    aTTTGCCTACAATATCAACAACAACGGTCACATGCTGCGTATCCTCGACCACGGCGCGATCTCCATGGGCGACAACTGGGACATGATGTACGt                                                     <  1:1367829/93‑1 (MQ=255)

                                     tttGCCTACAATATCAACAACAACGGTCACATGCTGCGTATCCTCGACCACGGCGCGATCTCCATGGGCGACAACTGGGACATGATGTACGTg                                                    <  1:2160640/93‑1 (MQ=255)

                                      ttGCCTACAATATCAACAACAACGGTCACATGCTGCGTATCCTCGACCACGGCGCGATCTCCATGGGCGACAACTGGGACATGATGTACGTgg                                                   >  1:4398749/1‑93 (MQ=255)

                                       tGCCTACAATATCAACAACAACGGTCACATGCTGCGTATCCTCGACCACGGCGCGATCTCCATGGGCGACAACTGGGACATGATGTACGTggg                                                  <  2:2591273/93‑1 (MQ=255)

                                         ccTACAATATCAACAACAACGGTCACATGCTGCGTATCCTCGACCACGGCGCGATCTCCATGGGCGACAACTGGGACATGATGTACGTGGGTa                                                >  2:456297/1‑93 (MQ=255)

                                          cTACATTATCGACAACAACGGTCACATGCTGCGTATCATCGAACACCACACGATCTCCATGGGCGATAACTGGAGTATGATGTACGTGGGTAt                                               >  2:2873502/1‑93 (MQ=255)

                                            aCAATATCAACAACAACGGTCACATGCTGCGTATCCTCGACCACGGCGCGATCTCCATGGGCGACAACTGGGACATGATGTACGTGGGTATGt                                             >  2:865884/1‑93 (MQ=255)

                                              aaTATCAACAACAACGGTCACATGCTGCGTATCCTCGACCACGGCGCGATCTCCATGGGCGACAACTGGGACATGATGTACGTGGGTATGTAc                                           >  1:3147089/1‑93 (MQ=255)

                                               atatCAACAACAACGGTCACATGCTGCGTATCCTCGACCACGGCGCGATCTCCATGGGCGACAACTGGGACATGATGTACGTGGGTATGTAcc                                          <  1:210148/93‑1 (MQ=255)

                                               atatCAACAACAACGGTCACATGCTGCGTATCCTCGACCACGGCGCGATCTCCATGGGCGACAACTGGGACATGATGTACGTGGGTATGTAcc                                          >  2:2176897/1‑93 (MQ=255)

                                                tatCAACAACAACGGTCACATGCTGCGTATCCTCGACCACGGCGCGATCTCCATGGGCGACAACTGGGACATGATGTACGTGGGTATGTACCa                                         >  1:2548039/1‑93 (MQ=255)

                                                 atCAACAACAACGGTCACATGCTGCGTATCCTCGACCACGGCGCGATCTCCATGGGCGACAACTGGGACATGATGTACGTGGGTATGTACCAg                                        >  1:4243523/1‑93 (MQ=255)

                                                 atCAACAACAACGGTCACATGCTGCGTATCCTCGACCACGGCGCGATCTCCATGGGCGACAACTGGGACATGATGTACGTGGGTATGTACCAg                                        <  2:1508633/93‑1 (MQ=255)

                                                  tCAACAACAACGGTCACATGCTGCGTATCCTCGACCACGGCGCGATCTCCATGGGCGACAACTGGGACATGATGTACGTGGGTATGTACCAgg                                       >  2:4775311/1‑93 (MQ=255)

                                                   caacaacaaCGGTCACATGCTGCGTATCCTCGACCACGGCGCGATCTCCATGGGCGACAACTGGGACATGATGTACGTGGGTATGTACCAGGa                                      <  1:3677582/93‑1 (MQ=255)

                                                    aacaacaacGGTCACATGCTGCGTATCCTCGACCACGGCGCGATCTCCATGGGCGACAACTGGGACATGATGTACGTGGGTATGTACCAGGat                                     <  1:1834133/93‑1 (MQ=255)

                                                       aacaacGGTCACATGCTGCGTATCCTCGACCACGGCGCGATCTCCATGGGCGACAACTGGGACATGATGTACGTGGGTATGTACCAGGATATc                                  <  1:5742236/93‑1 (MQ=255)

                                                       aacaacGGTCACATGCTGCGTATCCTCGACCACGGCGCGATCTCCATGGGCGACAACTGGGACATGATGTACGTGGGTATGTACCAGGATATc                                  <  2:1813524/93‑1 (MQ=255)

                                                       aacaacGGTCACATGCTGCGTATCCTCGACCACGGCGCGATCTCCATGGGCGACAACTGGGACATGATGTACGTGGGTATGTACCAGGATATc                                  >  1:5607328/1‑93 (MQ=255)

                                                        acaacGGTCACATGCTGCGTATCCTCGACCACGGCGCGATCTCCATGGGCGACAACTGGGACATGATGTACGTGGGTATGTACCAGGATATCa                                 >  2:4010392/1‑93 (MQ=255)

                                                        acaacGGTCACATGCTGCGTATCCTCGACCACGGCGCGATCTCCATGGGCGACAACTGGGACATGATGTACGTGGGTATGTACCAGGATATCa                                 >  1:5406170/1‑93 (MQ=255)

                                                         caacGGTCACATGCTGCGTATCCTCGACCACGGCGCGATCTCCATGGGCGACAACTGGGACATGATGTACGTGGGTATGTACCAGGATATCaa                                >  1:3354913/1‑93 (MQ=255)

                                                          aacGGTCACATGCTGCGTATCCTCGACCACGGCGCGATCTCCATGGGCGACAACTGGGACATGATGTACGTGGGTATGTACCAGGATATCAAc                               >  2:3201242/1‑93 (MQ=255)

                                                          aacGGTCACATGCTGCGTATCCTCGACCACGGCGCGATCTCCATGGGCGACAACTGGGACATGATGTACGTGGGTATGTACCAGGATATCAAc                               <  2:4703614/93‑1 (MQ=255)

                                                          aacGGTCACATGCTGCGTATCCTCGACCACGGCGCGATCTCCATGGGCGACAACTGGGACATGATGTACGTGGGTATGTACCAGGATATCAAc                               >  2:2062745/1‑93 (MQ=255)

                                                           acGGTCACATGCTGCGTATCCTCGACCACGGCGCGATCTCCATGGGCGACAACTGGGACATGATGTACGTGGGTATGTACCAGGATATCAACt                              >  2:2397909/1‑93 (MQ=255)

                                                           acGGTCACATGCTGCGTATCCTCGACCACGGCGCGATCTCCATGGGCGACAACTGGGACATGATGTACGTGGGTATGTACCAGGATATCAACt                              <  2:4441261/93‑1 (MQ=255)

                                                              gTCACATGCTGCGTATCCTCGACCACGGCGCGATCTCCATGGGCGACAACTGGGACATGATGTACGTGGGTATGTACCAGGATATCAACTggg                           >  2:4993217/1‑93 (MQ=255)

                                                              gTCACATGCTGCGTATCCTCGACCACGGCGCGATCTCCATGGGCGACAACTGGGACATGATGTACGTGGGTATGTACCAGGATATCAACTggg                           <  1:4726910/93‑1 (MQ=255)

                                                                cacaTGCTGCGTATCCTCGACCACGGCGCGATCTCCATGGGCGACAACTGGGACATGATGTACGTGGGTATGTACCAGGATATCAACTGGGAt                         <  1:4051562/93‑1 (MQ=255)

                                                                 acaTGCTGCGTATCCTCGACCACGGCGCGATCTCCATGGGCGACAACTGGGACATGATGTACGTGGGTATGTACCAGGATATCAACTGGGATa                        >  1:844268/1‑93 (MQ=255)

                                                                 acaTGCTGCGTATCCTCGACCACGGCGCGATCTCCATGGGCGACAACTGGGACATGATGTACGTGGGTATGTACCAGGATATCAACTGGGATa                        >  2:4467221/1‑93 (MQ=255)

                                                                  caTGCTGCGTATCCTCGACCACGGCGCGATCTCCATGGGCGACAACTGGGACATGATGTACGTGGGTATGTACCAGGATATCAACTGGGATaa                       <  2:4875731/93‑1 (MQ=255)

                                                                  caTGCTGCGTATCCTCGACCACGGCGCGATCTCCATGGGCGACAACTGGGACATGATGTACGTGGGTATGTACCAGGATATCAACTGGGATaa                       <  1:5029293/93‑1 (MQ=255)

                                                                  caTGCTGCGTATCCTCGACCACGGCGCGATCTCCATGGGCGACAACTGGGACATGATGTACGTGGGTATGTACCAGGATATCAACTGGGATaa                       <  2:5434050/93‑1 (MQ=255)

                                                                  caTGCTGCGTATCCTCGACCACGGCGCGATCTCCATGGGCGACAACTGGGACATGATGTACGTGGGTATGTACCAGGATATCAACTGGGATaa                       <  2:1137076/93‑1 (MQ=255)

                                                                   aTGCTGCGTATCCTCGACCACGGCGCGATCTCCATGGGCGACAACTGGGACATGATGTACGTGGGTATGTACCAGGATATCAACTGGGATAAc                      <  1:5656938/93‑1 (MQ=255)

                                                                    tgctgcGTATCCTCGACCACGGCGCGATCTCCATGGGCGACAACTGGGACATGATGTACGTGGGTATGTACCAGGATATCAACTGGGATAACg                     >  1:5844309/1‑93 (MQ=255)

                                                                      ctgcGTATCCTCGACCACGGCGCGATCTCCATGGGCGACAACTGGGACATGATGTACGTGGGTATGTACCAGGATATCAACTGGGATAACGAc                   <  1:2691628/93‑1 (MQ=255)

                                                                       tgcGTATCCTCGACCACGGCGCGATCTCCATGGGCGACAACTGGGACATGATGTACGTGGGTATGTACCAGGATATCAACTGGGATAACGACa                  <  2:1022948/93‑1 (MQ=255)

                                                                       tgcGTATCCTCGACCACGGCGCGATCTCCATGGGCGACAACTGGGACATGATGTACGTGGGTATGTACCAGGATATCAACTGGGATAACGACa                  <  1:481791/93‑1 (MQ=255)

                                                                        gcGTATCCTCGACCACGGCGCGATCTCCATGGGCGACAACTGGGACATGATGTACGTGGGTATGTACCAGGATATCAACTGGGATAACGACaa                 >  1:2040658/1‑93 (MQ=255)

                                                                         cGTATCCTCGACCACGGCGCGATCTCCATGGGCGACAACTGGGACATGATGTACGTGGGTATGTACCAGGATATCAACTGGGATAACGACAAc                >  2:368461/1‑93 (MQ=255)

                                                                          gTATCCTCGACCACGGCGCGATCTCCATGGGCGACAACTGGGACATGATGTACGTGGGTATGTACCAGGATATCAACTGGGATAACGACAACg               <  2:4937857/93‑1 (MQ=255)

                                                                           tATCCTCGACCACGGCGCGATCTCCATGGGCGACAACTGGGACATGATGTACGTGGGTATGTACCAGGATATCAACTGGGATAACGACAACgg              <  2:4330753/93‑1 (MQ=255)

                                                                              ccTCGACCACGGCGCGATCTCCATGGGCGACAACTGGGACATGATGTACGTGGGTATGTACCAGGATATCAACTGGGATAACGACAACGGCAc           <  2:5097851/93‑1 (MQ=255)

                                                                              ccTCGACCACGGCGCGATCTCCATGGGCGACAACTGGGACATGATGTACGTGGGTATGTACCAGGATATCAACTGGGATAACGACAACGGCAc           >  1:1619238/1‑93 (MQ=255)

                                                                              ccTCGACCACGGCGCGATCTCCATGGGCGACAACTGGGACATGATGTACGTGGGTATGTACCAGGATATCAACTGGGATAACGACAACGGCAc           >  2:6015257/1‑93 (MQ=255)

                                                                                  gACCACGGCGCGATCTCCATGGGCGACAACTGGGACATGATGTACGTGGGTATGTACCAGGATATCAACTGGGATAACGACAACGGCACCAAg       >  2:3492921/1‑93 (MQ=255)

                                                                                  gACCACGGCGCGATCTCCATGGGCGACAACTGGGACATGATGTACGTGGGTATGTACCAGGATATCAACTGGGATAACGACAACGGCACCAAg       >  1:3303933/1‑93 (MQ=255)

                                                                                   aCCACGGCGCGATCTCCATGGGCGACAACTGGGACATGATGTACGTGGGTATGTACCAGGATATCAACTGGGATAACGACAACGGCACCAAgt      >  2:3926687/1‑93 (MQ=255)

                                                                                   aCCACGGCGCGATCTCCATGGGCGACAACTGGGACATGATGTACGTGGGTATGTACCAGGATATCAACTGGGATAACGACAACGGCACCAAgt      <  2:5857287/93‑1 (MQ=255)

                                                                                    ccACGGCGCGATCTCCATGGGCGACAACTGGGACATGATGTACGTGGGTATGTACCAGGATATCAACTGGGATAACGACAACGGCACCAAgtg     <  2:1932314/93‑1 (MQ=255)

                                                                                     cACGGCGCGATCTCCATGGGCGACAACTGGGACATGATGTACGTGGGTATGTACCAGGATATCAACTGGGATAACGACAACGGCACCAAgtgg    >  2:5168774/1‑93 (MQ=255)

                                                                                     cACGGCGCGATCTCCATGGGCGACAACTGGGACATGATGTACGTGGGTATGTACCAGGATATCAACTGGGATAACGACAACGGCACCAAgtgg    >  1:5584037/1‑93 (MQ=255)

GTCGCAGGGTTCTGGCGTTGCATTTGATAACGAAAAATTTGCCTACAATATCAACAACAACGGTCACATGCTGCGTATCCTCGACCACGGTGCGATCTCCATGGGCGACAACTGGGACATGATGTACGTGGGTATGTACCAGGATATCAACTGGGATAACGACAACGGCACCAAGTGGTG  >  NC_000913/4246362‑4246541

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

CTGTCGCAGGGTTCTGGCGTTGCATTTGATAACGAAAAATTTGCCTACAATATCAACAACAACGGTCACATGCTGCGTATCCTCGACCACGGTGCGATCTCCATGGGCGACAACTGGGACATGATGTACGTGGGTATGTACCAGGATATCAACTGGGATAACGACAACGGCACCAAGTGGTGGACC  >  NC_000913/4246360‑4246545

CTGTCGCAGGGTTCTGGCGTTGCATTTGATAACGAAAAATTTGCCTACAATATCAACAACAACGGTCACATGCTGCGTATCCTCGACCACGGCG                                                                                              <  SRR4302533.499154/94‑1 (MQ=60)

CTGTCGCAGGGTTCTGGCGTTGCATTTGATAACGAAAAATTTGCCTACAATATCAACAACAACGGTCACATGCTGCGTATCCTCGACCACGGCG                                                                                              <  SRR4302533.47587/94‑1 (MQ=60)

  GTCGCAGGGTTCTGGCGTTGCATTTGATAACGAAAAATTTGCCTACAATATCAACAACAACGGTCACATGCTGCGTATCCTCGACCACGGCGCG                                                                                            <  SRR4302533.5516813/94‑1 (MQ=60)

  GTCGCAGGGTTCTGGCGTTGCATTTGATAACGAAAAATTTGCCTACAATATCAACAACAACGGTCACATGCTGCGTATCCTCGACCACGGCGCG                                                                                            <  SRR4302533.461446/94‑1 (MQ=60)

  GTCGCAGGGTTCTGGCGTTGCATTTGATAACGAAAAATTTGCCTACAATATCAACAACAACGGTCACATGCTGCGTATCCTCGACCACGGCGCG                                                                                            <  SRR4302533.1047622/94‑1 (MQ=60)

   TCGCAGGGTTCTGGCGTTGCATTTGATAACGAAAAATTTGCCTACAATATCAACAACAACGGTCACATGCTGCGTATCCTCGACCACGGCGCGA                                                                                           >  SRR4302533.963974/1‑94 (MQ=60)

   TCGCAGGGTTCTGGCGTTGCATTTGATAACGAAAAATTTGCCTACAATATCAACAACAACGGTCACATGCTGCGTATCCTCGACCACGGCGCGA                                                                                           <  SRR4302533.675530/94‑1 (MQ=60)

      CAGGTTTCTGGCGTTGCATTTGATAACGAAAAATTTGCCTACAATATCAACAACAACGGTCACATGCTGCGTATCCTCGACCACGGCGCGATCT                                                                                        <  SRR4302533.2351008/94‑1 (MQ=60)

      CAGGGTTCTGGCGTTGCATTTGATAACGAAAAATTTGCCTACAATATCAACAACAACGGTCACATGCTGCGTATCCTCGACCACGGCGCGATCT                                                                                        >  SRR4302533.3878454/1‑94 (MQ=60)

      CAGGGTTCTGGCGTTGCATTTGATAACGAAAAATTTGCCTACAATATCAACAACAACGGTCACATGCTGCGTATCCTCGACCACGGCGCGATCT                                                                                        <  SRR4302533.2617555/94‑1 (MQ=60)

       AGGGTTCTGGCGTTGCATTTGATAACGAAAAATTTGCCTACAATATCAACAACAACGGTCACATGCTGCGTATCCTCGACCACGGCGCGATCTC                                                                                       >  SRR4302533.3343040/1‑94 (MQ=60)

        GGGTTCTGGCGTTGCATTTGATAACGAAAAATTTGCCTACAATATCAACAACAACGGTCACATGCTGCGTATCCTCGACCACGGCGCGATCTCC                                                                                      <  SRR4302533.2238344/94‑1 (MQ=60)

         GGTTCTGGCGTTGCATTTGATAACGAAAAATTTGCCTACAATATCAACAACAACGGTCACATGCTGCGTATCCTCGACCACGGCGCGATCTCCA                                                                                     >  SRR4302533.5637743/1‑94 (MQ=60)

         GGTTCTGGCGTTGCATTTGATAACGAAAAATTTGCCTACAATATCAACAACAACGGTCACATGCTGCGTATCCTCGACCACGGCGCGATCTCCA                                                                                     >  SRR4302533.3660773/1‑94 (MQ=60)

         GGTTCTGGCGTTGCATTTGATAACGAAAAATTTGCCTACAATATCAACAACAACGGTCACATGCTGCGTATCCTCGACCACGCCGCGATCTCCA                                                                                     >  SRR4302533.5281624/1‑94 (MQ=60)

          GTTCTGGCGTTGCATTTGATAACGAAAAATTTGCCTACAATATCAACAACAACGGTCACATGCTGCGTATCCTCGACCACGGCGCGATCTCCAT                                                                                    <  SRR4302533.2238211/94‑1 (MQ=60)

          GTTCTGGCGTTGCATTTGATAACGAAAAATTTGCCTACAATATCAACAACAACGGTCACATGCTGCGTATCCTCGACCACGGCGCGATCTCCAT                                                                                    <  SRR4302533.3415810/94‑1 (MQ=60)

            TCTGGCGTTGCATTTGATAACGAAAAATTTGCCTACAATATCAACAACAACGGTCACATGCTGCGTATCCTCGACCACGGCGCGATCTCCATGG                                                                                  <  SRR4302533.29840/94‑1 (MQ=60)

            TCTGGCGTTGCATTTGATAACGAAAAATTTGCCTACAATATCAACAACAACGGTCACATGCTGCGTATCCTCGACCACGGCGCGATCTCCATGG                                                                                  <  SRR4302533.967796/94‑1 (MQ=60)

             TTGGCGTTGCATTTGATAACGAAAAATTTGCCTACAATATCAACAACAACGGTCACATGCTGCGTATCCTCGACCACGGCGCGATCTCCATGGG                                                                                 <  SRR4302533.3746730/94‑1 (MQ=60)

             CTGGCGTTGCATTTGATAACGAAAAATTTGCCTACAATATCAACAACAACGGTCACATGCTGCGTATCCTCGACCACGGCGCGATCTCCATGGG                                                                                 <  SRR4302533.1280499/94‑1 (MQ=60)

             CTGGCGTTGCATTTGATAACGAAAAATTTGCCTACAATATCAACAACAACGGTCACATGCTGCGTATCCTCGACCACGGCGCGATCTCCATGGG                                                                                 >  SRR4302533.5639585/1‑94 (MQ=60)

             CTGGCGTTGCATTTGATAACGAAAAATTTGCCTACAATATCAACAACAACGGTCACATGCTGCGTATCCTCGACCACGGCGCGATCTCCATGGG                                                                                 <  SRR4302533.566815/94‑1 (MQ=60)

              TGGCGTTGCATTTGATAACGAAAAATTTGCCTACAATATCAACAACAACGGTCACATGCTGCGTATCCTCGACCACGGCGCGATCTCCATGGGC                                                                                <  SRR4302533.6130268/94‑1 (MQ=60)

               GGCGTTGCATTTGATAACGAAAAATTTGCCTACAATATCAACAACAACGGTCACATGCTGCGTATCCTCGACCACGGCGCGATCTCCATGGGCG                                                                               <  SRR4302533.3551261/94‑1 (MQ=60)

               GGCGTTGCATTTGATAACGAAAAATTTGCCTACAATATCAACAACAACGGTCACATGCTGCGTATCCTCGACCACGGCGCGATCTCCATGGGCG                                                                               <  SRR4302533.3551260/94‑1 (MQ=60)

               GGCGTTGCATTTGATAACGAAAAATTTGCCTACAATATCAACAACAACGGTCACATGCTGCGTATCCTCGACCACGGCGCGATCTCCATGGGCG                                                                               <  SRR4302533.226198/94‑1 (MQ=60)

                GCGTTGCATTTGATAACGAAAAATTTGCCTACAATATCAACAACAACGGTCACATGCTGCGTATCCTCGACCACGGCGCGATCTCCATGGGCGA                                                                              >  SRR4302533.3465247/1‑94 (MQ=60)

                 CGTTGCATTTGATAACGAAAAATTTGCCTACAATATCAACAACAACGGTCACATGCTGCGTATCCTCGACCACGGCGCGATCTCCATGGGCGAC                                                                             >  SRR4302533.1548519/1‑94 (MQ=60)

                 CGTTGCATTTGATAACGAAAAATTTGCCTACAATATCAACAACAACGGTCACATGCTGCGTATCCTCGACCACGGCGCGATCTCCATGGGCGAC                                                                             >  SRR4302533.2997129/1‑94 (MQ=60)

                 CGTTGCATTTGATAACGAAAAATTTGCCTACAATATCAACAACAACGGTCACATGCTGCGTATCCTCGACCACGGCGCGATCTCCATGGGCGAC                                                                             <  SRR4302533.5581694/94‑1 (MQ=60)

                  GTTGCATTTGATAACGAAAAATTTGCCTACAATATCAACAACAACGGTCACATGCTGCGTATCCTCGACCACGGCGCGATCTCCATGGGCGACA                                                                            >  SRR4302533.54735/1‑94 (MQ=60)

                   TTGCATTTGATAACGAAAAATTTGCCTACAATATCAACAACAACGGTCACATGCTGCGTATCCTCGACCACGGCGCGATCTCCATGGGCGACAC                                                                           >  SRR4302533.5836394/1‑94 (MQ=60)

                   TTGCATTTGATAACGAAAAATTTGCCTACAATATCAACAACAACGGTCACATGCTGCGTATCCTCGACCACGGCGCGATCTCCATGGGCGACAA                                                                           >  SRR4302533.1943046/1‑94 (MQ=60)

                    TGCATTTGATAACGAAAAATTTGCCTACAATATCAACAACAACGGTCACATGCTGCGTATCCTCGACCACGGCGCGATCTCCATGGGCGACAAC                                                                          <  SRR4302533.2193710/94‑1 (MQ=60)

                    TGCATTTGATAACGAAAAATTTGCCTACAATATCAACAACAACGGTCACATGCTGCGTATCCTCGACCACGGCGCGATCTCCATGGGCGACAAC                                                                          >  SRR4302533.2833781/1‑94 (MQ=60)

                    TGCATTTGATAACGAAAAATTTGCCTACAATATCAACAACAACGGTCACATGCTGCGTATCCTCGACCACGGCGCGATCTCCATGGGCGACAAC                                                                          <  SRR4302533.3046954/94‑1 (MQ=60)

                    TGCATTTGATAACGAAAAATTTGCCTACAATATCAACAACAACGGTCACATGCTGCGTATCCTCGACCACGGCGCGATCTCCATGGGCGACAAC                                                                          >  SRR4302533.6076323/1‑94 (MQ=60)

                    TGCATTTCATAACGAAAAATTTGCCTACAATATCAACAACAACGGTCACATGCTGCGTATCCTCGACCACGGCGCGATCTCCATGGGCGACAAC                                                                          <  SRR4302533.1563085/94‑1 (MQ=60)

                     GCATTTGATAACGAAAAATTTGCCTACAATATCAACAACAACGGTCACATGCTGCGTATCCTCGACCACGGCGCGATCTCCATGGGCGACAACT                                                                         <  SRR4302533.1243626/94‑1 (MQ=60)

                     GCATTTGATAACGAAAAATTTGCCTACAATATCAACAACAACGGTCACATGCTGCGTATCCTCGACCACGGCGCGATCTCCATGGGCGACAACT                                                                         >  SRR4302533.753088/1‑94 (MQ=60)

                     GCATTTGATAACGAAAAATGTGCCTACAATATCAACAACAACGGTCACATGCTGCGTATCCTCGACCACGGCGCGATCTCCATGGGCGACAACT                                                                         <  SRR4302533.264513/94‑1 (MQ=60)

                      CATTTGATAACGAAAAATTTGCCTACAATATCAACAACAACGGTCACATGCTGCGTATCCTCGACCACGGCGCGATCTCCATGGGCGACAACTG                                                                        <  SRR4302533.4848933/94‑1 (MQ=60)

                      CATTTGATAACGAAAAATTTGCCTACAATATCAACAACAACGGTCACATGCTGCGTATCCTCGACCACGGCGCGATCTCCATGGGCGACAACTG                                                                        >  SRR4302533.133497/1‑94 (MQ=60)

                      CATTTGATAACGAAAAATTTGCCTACAATATCAACAACAACGGTCACATGCTGCGTATCCTCGACCACGGCGCGATCTCCATGGGCGACAACTG                                                                        >  SRR4302533.4719544/1‑94 (MQ=60)

                      CATTTGATAACGAAAAATTTGCCTACAATATCAACAACAACGGTCACATGCTGCGTATCCTCGACCACGGCGCGATCTCCATGGGCGACAACTG                                                                        >  SRR4302533.3630064/1‑94 (MQ=60)

                      CATTTGATAACGAAAAATTTGCCTACAATATCAACAACAACGGTCACATGCTGCGTATCCTCGACCACGGCGCGATCTCCATGGGCGACAACTG                                                                        >  SRR4302533.1457957/1‑94 (MQ=60)

                       ATTTGATAACGAAAAATTTGCCTACAATATCAACAACAACGGTCACATGCTGCGTATCCTCGACCACGGCGCGATCTCCATGGGCGACAACTGG                                                                       <  SRR4302533.2540348/94‑1 (MQ=60)

                       ATTTGATAACGAAAAATTTGCCTACAATATCAACAACAACGGTCACATGCTGCGTATCCTCGACCACGGCGCGATCTCCATGGGCGACAACTGG                                                                       >  SRR4302533.4404731/1‑94 (MQ=60)

                       ATTTGATAACGAAAAATTTGCCTACAATATCAACAACAACGGTCACATGCTGCGTATCCTCGACCACGGCGCGATCTCCATGGGCGACAACTGG                                                                       >  SRR4302533.628559/1‑94 (MQ=60)

                       ATTTGATAACGAAAAATTTGCCTACAATATCAACAACAACGGTCACATGCTGCGTATCCTCGACCACGGCGCGATCTCCATGGGCGACAACTGG                                                                       >  SRR4302533.2963605/1‑94 (MQ=60)

                         TTGATAACGAAAAATTTGCCTACAATATCAACAACAACGGTCACATGCTGCGTATCCTCGACCACGGCGCGATCTCCATGGGCGACAACTGGGA                                                                     <  SRR4302533.3220139/94‑1 (MQ=60)

                          TGATAACGAAAAATTTGCCTACAATATCAACAACAACGGTCACATGCTGCGTATCCTCGACCACGGCGCGATCTCCATGGGCGACAACTGGGAC                                                                    <  SRR4302533.3628632/94‑1 (MQ=60)

                          TGATAACGAAAAATTTGCCTACAATATCAACAACAACGGTCACATGCTGCGTATCCTCGACCACGGCGCGATCTCCATGGGCGACAACTGGGAC                                                                    >  SRR4302533.5147378/1‑94 (MQ=60)

                          TGATAACGAAAAATTTGCCTACAATATCAACAACAACGGTCACATGCTGCGTATCCTCGACCACGGCGCGATCTCCATGGGCGACAACTGGGAC                                                                    <  SRR4302533.5565243/94‑1 (MQ=60)

                          TGATAACGAAAAATTTGCCTACAATATCAACAACAACGGTCACATGCTGCGTATCCTCGACCACGGCGCGATCTCCATGGGCGACAACTGGGAC                                                                    >  SRR4302533.1930698/1‑94 (MQ=60)

                           GATAACGAAAAATTTGCCTACAATATCAACAACAACGGTCACATGCTGCGTATCCTCGACCACGGCGCGATCTCCATGGGCGACAACTGGGACA                                                                   >  SRR4302533.4028837/1‑94 (MQ=60)

                           GATAACGAAAAATTTGCCTACAATATCAACAACAACGGTCACATGCTGCGTATCCTCGACCACGGCGCGATCTCCATGGGCGACAACTGGGACA                                                                   <  SRR4302533.4744569/94‑1 (MQ=60)

                           GATAACGAAAAATTTGCCTACAATATCAACAACAACGGTCACATGCTGCGTATCCTCGACCACGGCGCGATCTCCATGGGCGACAACTGGGACA                                                                   <  SRR4302533.6031114/94‑1 (MQ=60)

                           GATAACGAAAAATTTGCCTACAATATCAACAACAACGGTCACATGCTGCGTATCCTCGACCACGGCGCGATCTCCATGGGCGACAACTGGGACA                                                                   <  SRR4302533.2078785/94‑1 (MQ=60)

                            ATAACGAAAAATTTGCCTACAATATCAACAACAACGGTCACATGCTGCGTATCCTCGACCACGGCGCGATCTCCATGGGCGACAACTGGGACAT                                                                  >  SRR4302533.3695776/1‑94 (MQ=60)

                            ATAACGAAAAATTTGCCTACAATATCAACAACAACGGTCACATGCTGCGTATCCTCGACCACGGCGCGATCTCCATGGGCGACAACTGGGACAT                                                                  >  SRR4302533.4119422/1‑94 (MQ=60)

                             TAACGAAAAATTTGCCTACAATATCAACAACAACGGTCACATGGTGCGTATCCTCGACCACGGCGCGATCTCCATGGGCGACAACTGGGACATG                                                                 >  SRR4302533.3402242/1‑94 (MQ=60)

                             TAACGAAAAATTTGCCTACAATATCAACAACAACGGTCACATGCTGCGTATCCTCGACCACGGCGCGATCTCCATGGGCGACAACTGGGACATG                                                                 <  SRR4302533.4139798/94‑1 (MQ=60)

                             TAACGAAAAATTTGCCTACAATATCAACAACAACGGTCACATGCTGCGTATCCTCGACCACGGCGCGATCTCCATGGGCGACAACTGGGACATG                                                                 >  SRR4302533.5501794/1‑94 (MQ=60)

                              AACGAAAAATTTGCCTACAATATCAACAACAACGGTCACATGCTGCGTATCCTCGACCACGGCGCGATCTCCATGGGCGACAACTGGGACATGA                                                                >  SRR4302533.2122021/1‑94 (MQ=60)

                                CGAAAAATTTGCCTACAATATCAACAACAACGGTCACATGCTGCGTATCCTCGACCACGGCGCGATCTCCATGGGCGACAACTGGGACATGATG                                                              >  SRR4302533.2577899/1‑94 (MQ=60)

                                CGAAAAATTTGCCTACAATATCAACAACAACGGTCACATGCTGCGTATCCTCGACCACGGCGCGATCTCCATGGGCGACAACTGGGACATGATG                                                              <  SRR4302533.6168633/94‑1 (MQ=60)

                                     AATTTGCCTACAATATCAACAACAACGGTCACATGCTGCGTATCCTCGACCACGGCGCGATCTCCATGGGCGACAACTGGGACATGATGTACGG                                                         >  SRR4302533.4957489/1‑94 (MQ=60)

                                      ATTTGCCTACAATATCAACAACAACGGTCACATGCTGCGTATCCTCGACCACGGCGCGATCTCCATGGGCGACAACTGGGACATGATGTACGTG                                                        <  SRR4302533.5019297/94‑1 (MQ=60)

                                      ATTTGCCTACAATATCAACAACAACGGTCACATGCTGCGTATCCTCGACCACGGCGCGATCTCCATGGGCGACAACTGGGACATGATGTACGTG                                                        <  SRR4302533.1376054/94‑1 (MQ=60)

                                      ATTTGCCTACAATATCAACAACAACGGTCACATGCTGCGTATCCTCGACCACGGCGCGATCTCCATGGGCGACAACTGGGACATGATGTACGTG                                                        <  SRR4302533.3574208/94‑1 (MQ=60)

                                      ATTTGCCTACAATATCAACAACAACGGTCACATGCTGCGTATCCTCCACCACGGCGCGATCTCCATGGGCGACAACTGGGACATGATGTACGTG                                                        >  SRR4302533.4112653/1‑94 (MQ=60)

                                       TTTGCCTACAATATCAACAACAACGGTCACATGCTGCGTATCCTCGACCACGGCGCGATCTCCATGGGCGACAACTGGGACATGATGTACGTGG                                                       <  SRR4302533.210784/94‑1 (MQ=60)

                                       TTTGCCTACAATATCAACAACAACGGTCACATGCTGCGTATCCTCGACCACGGCGCGATCTCCATGGGCGACAACTGGGACATGATGTACGTGG                                                       >  SRR4302533.4425305/1‑94 (MQ=60)

                                       TTTGCCTACAATATCAACAACAACGGTCACATGCTGCGTATCCTCGACCACGGCGCGATCTCCATGGGCGACAACTGGGACATGATGTACGTGG                                                       <  SRR4302533.1370550/94‑1 (MQ=60)

                                       TTTGCCTACAATATCAACAACAACGGTCACATGCTGCGTATCCTCGACCACGGCGCGATCTCCATGGGCGACAACTGGGACATGATGTACGTGG                                                       <  SRR4302533.2173939/94‑1 (MQ=60)

                                       TTTGCCTACAATATCAACAACAACGGTCACATGCTGCGTATCCTCGACCACGGCGCGATCTCCATGGGCGACAACTGGGACATGATGTACGTGG                                                       <  SRR4302533.6152577/94‑1 (MQ=60)

                                        TTGCCTACAATATCAACAACAACGGTCACATGCTGCGTATCCTCGACTACGGCGCGATCTCCATGGGCGACAACTGGGACATGATGTACGTGGG                                                      <  SRR4302533.4111388/94‑1 (MQ=60)

                                        TTGCCTACAATATCAACAACAACGGTCACATGCTGCGTATCCTCGACCACGGCGCGATCTCCATGGGCGACAACTGGGACATGATGTACGTGGG                                                      <  SRR4302533.3815966/94‑1 (MQ=60)

                                         TGCCTACAATATCAACAACAACGGTCACATGCTGCGTATCCTCGACCACGGCGCGATCTCCATGGGCGACAACTGGGACATGATGTACGTGGGT                                                     <  SRR4302533.2373473/94‑1 (MQ=60)

                                         TGCCTACAATATCAACAACAACGGTCACATGCTGCGTATCCTCGACCACGGCGCGATCTCCATGGGCGACAACTGGGACATGATGTACGTGGGT                                                     >  SRR4302533.5013614/1‑94 (MQ=60)

                                          GCCTACAATATCAACAACAACGGTCACATGCTGCGTATCCTCGACCACGGCGCGATCTCCATGGGCGACAACTGGGACATGATGTACGTGGGTA                                                    <  SRR4302533.818895/94‑1 (MQ=60)

                                          GCCTACAATATCAACAACAACGGTCACATGCTGCGTATCCTCGACCACGGCGCGATCTCCATGGGCGACAACTGGGACATGATGTACGTGGGTA                                                    <  SRR4302533.3988253/94‑1 (MQ=60)

                                           cctaaaacaaCAACAACAACGGTCACATGCTGCGTATCCTCGACCACGGCGCGATCTCCATGGGCGACAACTGGGACATGATGTACGTGGGTAT                                                   <  SRR4302533.1562102/84‑1 (MQ=60)

                                           CCTACAATATCAACAACAACGGTCACATGCTGCGTATCCTCGACCACGGCGCGATCTCCATGGGCGACAACTGGGACATGATGTACGTGGGTAT                                                   <  SRR4302533.1976249/94‑1 (MQ=60)

                                           CCTACAATATCAACAACAACGGTCACATGCTGCGTATCCTCGACCACGGCGCGATCTCCATGGGCGACAACTGGGACATGATGTACGTGGGTAT                                                   <  SRR4302533.4168622/94‑1 (MQ=60)

                                            CTACAATATCAACAACAACGGTCACATGCTGCGTATCCTCGACCACGGCGCGATCTCCATGGGCGACAACTGGGACATGATGTACGTGGGTATG                                                  <  SRR4302533.4952002/94‑1 (MQ=60)

                                            CTACAATATCAACAACAACGGTCACATGCTGCGTATCCTCGACCACGGCGCGATCTCCATGGGCGACAACTGGGACATGATGTACGTGGGTATG                                                  >  SRR4302533.4395262/1‑94 (MQ=60)

                                            CTACAATATCAACAACAACGGTCACATGCTGCGTATCCTCGACCACGGCGCGATCTCCATGGGCGACAACTGGGACATGATGTACGTGGGTATG                                                  >  SRR4302533.1919071/1‑94 (MQ=60)

                                              ACAATATCAACAACAACGGTCACATGCTGCGTATCCTCGACCACGGCGCGATCTCCATGGGCGACAACTGGGACATGATGTACGTGGGTATGTA                                                >  SRR4302533.5730056/1‑94 (MQ=60)

                                               CAATATCAACAACAACGGTCACATGCTGCGTATCCTCGACCACGGCGCGATCTCCATGGGCGACAACTGGGACATGATGTACGTGGGTATGTAC                                               >  SRR4302533.933013/1‑94 (MQ=60)

                                               CAATATCAACAACAACGGTCACATGCTGCGTATCCTCGACCACGGCGCGATCTCCATGGGCGACAACTGGGACATGATGTACGTGGGTATGTAC                                               >  SRR4302533.3166439/1‑94 (MQ=60)

                                                AATATCAACAACAACGGTCACATGCTGCGTATCCTCGACCACGGCGCGATCTCCATGGGCGACAACTGGGACATGATGTACGTGGGTATGTACC                                              <  SRR4302533.5470249/94‑1 (MQ=60)

                                                AATATCAACAACAACGGTCACATGCTGCGTATCCTCGACCACGGCGCGATCTCCATGGGCGACAACTGGGACATGATGTACGTGGGTATGTACC                                              >  SRR4302533.3144916/1‑94 (MQ=60)

                                                 ATATCAACAACATCGGTCACATGCTGCGTATCCTCGTCCACGGCGCGATCTCCATGGGCGACAACTGGGACATGATGTACGTGGGTATGTACCA                                             <  SRR4302533.5414327/94‑1 (MQ=60)

                                                 ATATCAACAACAACGGTCACATGCTGCGTATCCTCGACCACGGCGCGATCTCCATGGGCGACAACTGGGACATGATGTACGTGGGTATGTACCA                                             >  SRR4302533.2563722/1‑94 (MQ=60)

                                                 ATATCAACAACAACGGTCACATGCTGCGTATCCTCGACCACGGCGCGATCTCCATGGGCGACAACTGGGACATGATGTACGTGGGTATGTACCA                                             <  SRR4302533.1748795/94‑1 (MQ=60)

                                                 ATATCAACAACAACGGTCACATGCTGCGTATCCTCGACCACGGCGCGATCTCCATGGGCGACAACTGGGACATGATGTACGTGGGTATGTACCA                                             >  SRR4302533.900641/1‑94 (MQ=60)

                                                 ATATCAACAACAACGGTCACATGCTGCGTATCCTCGACCACGGCGCGATCTCCATGGGCGACAACTGGGACATGATGTACGTGGGTATGTACCA                                             >  SRR4302533.5814384/1‑94 (MQ=60)

                                                 ATATCAACAACAACGGTCACATGCTGCGTATCCTCGACCACGGCGCGATCTCCATGGGCGACAACTGGGACATGATGTACGTGGGTATGTACCA                                             <  SRR4302533.211475/94‑1 (MQ=60)

                                                  TATCAACAACAACGGTCACATGCTGCGTATCCTCGACCACGGCGCGATCTCCATGGGCGACAACTGGGACATGATGTACGTGGGTATGTACCAG                                            >  SRR4302533.4269149/1‑94 (MQ=60)

                                                  TATCAACAACAACGGTCACATGCTGCGTATCCTCGACCACGGCGCGATCTCCATGGGCGACAACTGGGACATGATGTACGTGGGTATGTACCAG                                            <  SRR4302533.5611155/94‑1 (MQ=60)

                                                   ATCAACAACAACGGTCACATGCTGCGTATCCTCGACCACGGCGCGATCTCCATGGGCGACAACTGGGACATGATGTACGTGGGTATGTACCAGG                                           <  SRR4302533.5650856/94‑1 (MQ=60)

                                                     CAACAACAACGGTCACATGCTGCGTATCCTCGACCACGGCGCGATCTCCATGGGCGACAACTGGGACATGATGTACGTGGGTATGTACCAGGAT                                         <  SRR4302533.3700186/94‑1 (MQ=60)

                                                      AACAACAACGGTCACATGCTGCGTATCCTCGACCACGGCGCGATCTCCATGGGCGACAACTGGGACATGATGTACGTGGGTATGTACCAGGATA                                        <  SRR4302533.5893349/94‑1 (MQ=60)

                                                      AACAACAACGGTCACATGCTGCGTATCCTCGACCACGGCGCGATCTCCATGGGCGACAACTGGGACATGATGTACGTGGGTATGTACCAGGATA                                        <  SRR4302533.1845328/94‑1 (MQ=60)

                                                       ACAACAACGGTCACATGCTGCGTATCCTCGACCACGGCGCGATCTCCATGGGCGACAACTGGGACATGATGTACGTGGGTATGTACCAGGATAT                                       >  SRR4302533.4528220/1‑94 (MQ=60)

                                                        CAACAACGGTCACATGCTGCGTATCCTCGACCACGGCGCGATCTCCATGGGCGACAACTGGGACATGATGTACGTGGGTATGTACCAGGATATC                                      >  SRR4302533.5522703/1‑94 (MQ=60)

                                                        CAACAACGGTCACATGCTGCGTATCCTCGACCACGGCGCGATCTCCATGGGCGACAACTGGGACATGATGTACGTGGGTATGTACCAGGATATC                                      >  SRR4302533.2814836/1‑94 (MQ=60)

                                                        CAACAACGGTCACATGCTGCGTATCCTCGACCACGGCGCGATCTCCATGGGCGACAACTGGGACATGATGTACGTGGGTATGTACCAGGATATC                                      >  SRR4302533.5641212/1‑94 (MQ=60)

                                                         AACAACGGTCACATGCTGCGTATCCTCGACCACGGCGCGATCTCCATGGGCGACAACTGGGACATGATGTACGTGGGTATGTACCAGGATATCA                                     >  SRR4302533.2645664/1‑94 (MQ=60)

                                                         AACAACGGTCACATGCTGCGTATCCTCGACCACGGCGCGATCTCCATGGGCGACAACTGGGACATGATGTACGTGGGTATGTACCAGGATATCA                                     <  SRR4302533.5776886/94‑1 (MQ=60)

                                                         AACAACGGTCACATGCTGCGTATCCTCGACCACGGCGCGATCTCCATGGGCGACAACTGGGACATGATGTACGTGGGTATGTACCAGGATATCA                                     >  SRR4302533.5438655/1‑94 (MQ=60)

                                                          ACAACGGTCACATGCTGCGTATCCTCGACCACGGCGCGATCTCCATGGGCGACAACTGGGACATGATGTACGTGGGTATGTACCAGGATATCAA                                    >  SRR4302533.3375644/1‑94 (MQ=60)

                                                           CAACGGTCACATGCTGCGTATCCTCGACCACGGCGCGATCTCCATGGGCGACAACTGGGACATGATGTACGTGGGTATGTACCAGGATATCAAC                                   <  SRR4302533.3646594/94‑1 (MQ=60)

                                                             ACGGTCACATGCTGCGTATCCTCGACCACGGCGCGATCTCCATGGGCGACAACTGGGACATGATGTACGTGGGTATGTACCAGGATATCAACTG                                 >  SRR4302533.3175807/1‑94 (MQ=60)

                                                             ACGGTCACATGCTGCGTATCCTCGACCACGGCGCGATCTCCATGGGCGACAACTGGGACATGATGTACGTGGGTATGTACCAGGATATCAACTG                                 <  SRR4302533.5994828/94‑1 (MQ=60)

                                                                GTCACATGCTGCGTATCCTCGACCACGGCGCGATCTCCATGGGCGACAACTGGGACATGATGTACGTGGGTATGTACCAGGATATCAACTGGGA                              <  SRR4302533.1648930/94‑1 (MQ=60)

                                                                GTCACATGCTGCGTATCCTCGACCACGGCGCGATCTCCATGGGCGACAACTGGGACATGATGTACGTGGGTATGTACCAGGATATCAACTGGGA                              <  SRR4302533.4755567/94‑1 (MQ=60)

                                                                GTCACATGCTGCGTATCCTCGACCACGGCGCGATCTCCATGGGCGACAACTGGGACATGATGTACGTGGGTATGTACCAGGATATCAACTGGGA                              >  SRR4302533.4828230/1‑94 (MQ=60)

                                                                GTCACATGCTGCGTATCCTCGACCACGGCGCGATCTCCATGGGCGACAACTGGGACATGATGTACGTGGGTATGTACCAGGATATCAACTGGGA                              >  SRR4302533.3511743/1‑94 (MQ=60)

                                                                 TCACATGCTGCGTATCCTCGACCACGGCGCGATCTCCATGGGCGACAACTGGGACATGATGTACGTGGGTATGTACCAGGATATCAACTGGGAT                             <  SRR4302533.2864845/94‑1 (MQ=60)

                                                                 TCACATGCTGCGTATCCTCGACCACGGCGCGATCTCCATGGGCGACAACTGGGACATGATGTACGTGGGTATGTACCAGGATATCAACTAGGAT                             <  SRR4302533.5719846/94‑1 (MQ=60)

                                                                  CACATGCTGCGTATCCTCGACCACGGCGCGATCTCCATGGGCGACAACTGGGACATGATGTACGTGGGTATGTACCAGGATATCAACTGGGATA                            >  SRR4302533.849204/1‑94 (MQ=60)

                                                                  CACATGCTGCGTATCCTCGACCACGGCGCGATCTCCATGGGCGACAACTGGGACATGATGTACGTGGGTATGTACCAGGATATCAACTGGGATA                            <  SRR4302533.4076239/94‑1 (MQ=60)

                                                                  CACATGCTGCGTATCCTCGACCACGGCGCGATCTCCATGGGCGACAACTGGGACATGATGTACGTGGGTATGTACCAGGATATAAACTGGGATA                            >  SRR4302533.199085/1‑94 (MQ=60)

                                                                   ACATGCTGCGTATCCTCGACCACGGCGCGATCTCCATGGGCGACAACTGGGACATGATGTACGTGGGTATGTACCAGGATATCAACTGGGATAA                           <  SRR4302533.2420905/94‑1 (MQ=60)

                                                                    CATGCTGCGTATCCTCGACCACGGCGCGATCTCCATGGGCGACAACTGGGACATGATGTACGTGGGTATGTACCAGGATATCAACTGGGATAAC                          <  SRR4302533.5059743/94‑1 (MQ=60)

                                                                    CATGCTGCGTATCCTCGACCACGGCGCGATCTCCATGGGCGACAACTGGGACATGATGTACGTGGGTATGTACCAGGATATCAACTGGGATAAC                          >  SRR4302533.2953925/1‑94 (MQ=60)

                                                                     ATGCTGCGTATCCTCGACCACGGCGCGATCTCCATGGGCGACAACTGGGACATGATGTACGTGGGTATGTACCAGGATATCAACTGGGATAACG                         >  SRR4302533.5879642/1‑94 (MQ=60)

                                                                     ATGCTGCGTATCCTCGACCACGGCGCGATCTCCATGGGCGACAACTGGGACATGATGTACGTGGGTATGTACCAGGATATCAACTGGGATAACG                         >  SRR4302533.808505/1‑94 (MQ=60)

                                                                     ATGCTGCGTATCCTCGACCACGGCGCGATCTCCATGGGCGACAACTGGGACATGATGTACGTGGGTATGTACCAGGATATCAACTGGGATAACG                         <  SRR4302533.4453153/94‑1 (MQ=60)

                                                                     ATGCTGCGTATCCTCGACCACGGCGCGATCTCCATGGGCGACAACTGGGACATGATGTACGTGGGTATGTACCAGGATATCAACTGGGATAACG                         <  SRR4302533.5691123/94‑1 (MQ=60)

                                                                       GCTGCGTATCCTCGACCACGGCGCGATCTCCATGGGCGACAACTGGGACATGATGTACGTGGGTATGTACCAGGATATCAACTGGGATAACGAC                       <  SRR4302533.5117512/94‑1 (MQ=60)

                                                                       GCTGCGTATCCTCGACCACGGCGCGATCTCCATGGGCGACAACTGGGACATGATGTACGTGGGTATGTACCAGGATATCAACTGGGATAACGAC                       <  SRR4302533.6093945/94‑1 (MQ=60)

                                                                       GCTGCGTATCCTCGACCACGGCGCGATCTCCATGGGCGACAACTGGGACATGATGTACGTGGGTATGTACCAGGATATCAACTGGGATAACGAA                       >  SRR4302533.647498/1‑94 (MQ=60)

                                                                        CTGCGTATCCTCGACCACGGCGCGATCTCCATGGGCGACAACTGGGACATGATGTACGTGGGTATGTACCAGGATATCAACTGGGATAACGACA                      <  SRR4302533.2708125/94‑1 (MQ=60)

                                                                         TGCGTATCCTCGACCACGGCGCGATCTCCATGGGCGACAACTGGGACATGATGTACGTGGGTATGTACCAGGATATCAACTGGGATAACGACAA                     <  SRR4302533.5526426/94‑1 (MQ=60)

                                                                         TGCGTATCCTCGACCACGGCGCGATCTCCATGGGCGACAACTGGGACATGATGTACGTGGGTATGTACCAGGATATCAACTGGGATAACGACAA                     >  SRR4302533.2053236/1‑94 (MQ=60)

                                                                         TGCGTATCCTCGACCACGGCGCGATCTCCATGGGCGACAACTGGGACATGATGTACGTGGGTATGTACCAGGATATCAACTGGGATAACGACAA                     <  SRR4302533.484669/94‑1 (MQ=60)

                                                                          GCGTATCCTCGACCACGGCGCGATCTCCATGGGCGACAACTGGGACATGATGTACGTGGGTATGTACCAGGATATCAACTGGGATAACGACAAC                    <  SRR4302533.2703904/94‑1 (MQ=60)

                                                                            GTATCCTCGACCACGGCGCGATCTCCATGGGCGACAACTGGGACATGATGTACGTGGGTATGTACCAGGATATCAACTGGGATAACGACAACGG                  >  SRR4302533.1381452/1‑94 (MQ=60)

                                                                              ATCCTCGACCACGGCGCGATCTCCATGGGCGACAACTGGGACATGATGTACGTGGGTATGTACCAGGATATCAACTGGGATAACGACAACGGCA                <  SRR4302533.335681/94‑1 (MQ=60)

                                                                               TCCTCGACCACGGCGCGATCTCCATGGGCGACAACTGGGACATGATGTACGTGGGTATGTACCAGGATATCAACTGGGATAACGACAACGGCAC               >  SRR4302533.1629047/1‑94 (MQ=60)

                                                                                CCTCGACCACGGCGCGATCTCCATGGGCGACAACTGGGACATGATGTACGTGGGTATGTACCAGGATATCAACTGGGATAACGACAACGGCACC              <  SRR4302533.2775105/94‑1 (MQ=60)

                                                                                CCTCGACCACGGCGCGATCTCCATGGGCGACAACTGGGACATGATGTACGTGGGTATGTACCAGGATATCAACTGGGATAACGACAACGGCACC              >  SRR4302533.1350405/1‑94 (MQ=60)

                                                                                 CTCGACCACGGCGCGATCTCCATGGGCGACAACTGGGACATGATGTACGTGGGTATGTACCAGGATATCAACTGGGATAACGACAACGGCACCA             <  SRR4302533.1262092/94‑1 (MQ=60)

                                                                                  TCGACCACGGCGCGATCTCCATGGGCGACAACTGGGACATGATGTACGTGGGTATGTACCAGGATATCAACTGGGATAACGACAACGGCACCAA            >  SRR4302533.1176250/1‑94 (MQ=60)

                                                                                   CGACCACGGCGCGATCTCCATGGGCGACAACTGGGACATGATGTACGTGGGTATGTACCAGGATATCAACTGGGATAACGACAACGGCACCAAG           >  SRR4302533.3324337/1‑94 (MQ=60)

                                                                                    GACCACGGCGCGATCTCCATGGGCGACAACTGGGACATGATGTACGTGGGTATGTACCAGGATATCAACTGGGATAACGACAACGGCACCAAGT          >  SRR4302533.2127460/1‑94 (MQ=60)

                                                                                     ACCACGGCGCGATCTCCATGGGCGACAACTGGGACATGATGTACGTGGGTATGTACCAGGATATCAACTGGGATAACGACAACGGCACCAAGTG         <  SRR4302533.5074174/94‑1 (MQ=60)

                                                                                      CCACGGCGCGATCTCCATGGGCGACAACTGGGACATGATGTACGTGGGTATGTACCAGGATATCAACTGGGATAACGACAACGGCACCAAGTGG        >  SRR4302533.5617777/1‑94 (MQ=60)

                                                                                       CACGGCGCGATCTCCATGGGCGACAACTGGGACATGATGTACGTGGGTATGTACCAGGATATCAACTGGGATAACGACAACGGCACCAAGTGGT       >  SRR4302533.1140308/1‑94 (MQ=60)

                                                                                       CACGGCGCGATCTCCATGGGCGACAACTGGGACATGATGTACGTGGGTATGTACCAGGATATCAACTGGGATAACGACAACGGCACCAAGTGGT       <  SRR4302533.2966048/94‑1 (MQ=60)

                                                                                       CACGGCGCGATCTCCATGGGCGACAACTGGGACATGATGTACGTGGGTATGTACCAGGATATCAACTGGGATAACGACAACGGCACCAAGTGGT       <  SRR4302533.2300091/94‑1 (MQ=60)

                                                                                       CACGGCGCGATCTCCATGGGCGACAACTGGGACATGATGTACGTGGGGATGTACCAGGATATCAACTGGGATAACGACAACGGCACCAAGTGGG       >  SRR4302533.4821557/1‑94 (MQ=60)

                                                                                         CGGCGCGATCTCCATGGGCGACAACTGGGACATGATGTACGTGGGTATGTACCAGGATATCAACTGGGATAACGACAACGGCACCAAGTGGTGG     >  SRR4302533.3776344/1‑94 (MQ=60)

                                                                                          GGCGCGATCTCCATGGGCGACAACTGGGACATGATGTACGTGGGTATGTACCAGGATATCAACTGGGATAACGACAACGGCACCAAGTGGTGGA    <  SRR4302533.439285/94‑1 (MQ=60)

                                                                                          GGCGCGATCTCCATGGGCGACAACTGGGACATGATGTACGTGGGTATGTACCAGGATATCAACTGGGATAACGACAACGGCACCAAGTGGTGGA    <  SRR4302533.1565161/94‑1 (MQ=60)

                                                                                           GCGCGATCTCCATGGGCGACAACTGGGACATGATGTACGTGGGTATGTACCAGGATATCAACTGGGATAACGACAACGGCACCAAGTGGTGGAC   <  SRR4302533.6180754/94‑1 (MQ=60)

                                                                                           GCGCGATCTCCATGGGCGACAACTGGGACATGATGTACGTGGGTATGTACCAGGATATCAACTGGGATAACGACAACGGCACCAAGTGGTGGAC   <  SRR4302533.3271233/94‑1 (MQ=60)

                                                                                            CGCGATCTCCATGGGCGACAACTGGGACATGATGTACGTGGGTATGTACCAGGATATCAACTGGGATAACGACAACGGCACCAAGTGGTGGACC  <  SRR4302533.3367090/94‑1 (MQ=60)

                                                                                            CGCGATCTCCATGGGCGACAACTGGGACATGATGTACGTGGGTATGTACCAGGATATCAACTGGGATAACGACAACGGCACCAAGTGGTGGACC  <  SRR4302533.2482617/94‑1 (MQ=60)

                                                                                            CGCGATCTCCATGGGCGACAACTGGGACATGATGTACGTGGGTATGTACCAGGATATCAACTGGGATAACGACAACGGCACCAAGTGGTGGACC  <  SRR4302533.5511520/94‑1 (MQ=60)

CTGTCGCAGGGTTCTGGCGTTGCATTTGATAACGAAAAATTTGCCTACAATATCAACAACAACGGTCACATGCTGCGTATCCTCGACCACGGTGCGATCTCCATGGGCGACAACTGGGACATGATGTACGTGGGTATGTACCAGGATATCAACTGGGATAACGACAACGGCACCAAGTGGTGGACC  >  NC_000913/4246360‑4246545

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`