Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1624	250.6	12620048	98.0%	12367647	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,250,949	A→G	I119V (ATT→GTT)	ubiA →	4‑hydroxybenzoate‑octaprenyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,250,949	0	A	G	100.0%	838.5 / NA	258	I119V (ATT→GTT)	ubiA	4‑hydroxybenzoate‑octaprenyltransferase
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (146/112); total (146/112)

Only 100 of 262 total aligned reads displayed.

GGCGCGGTAACAGAGAAAGAGGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCATTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTACCGCAAGTGGTGCTGGGCGCG  >  NC_000913/4250859‑4251038

ggcgCGGTAACAGAGAAAGAGGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGtt                                                                                         >  1:2719580/1‑93 (MQ=255)

  cgcgATAATAGAGAAATAGGCGCGCATGCTGTTTTTCGTTCTGGTCCTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACTGTTCt                                                                                       <  2:2918423/93‑1 (MQ=255)

   gcgGTAACAGAGAAAGAGGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCtg                                                                                      >  2:3771482/1‑93 (MQ=255)

   gcgGTAACAGAGAAAGAGGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCtg                                                                                      >  1:1600181/1‑93 (MQ=255)

     ggTAACAGAGAAAGAGGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCtgtt                                                                                    >  1:5489385/1‑93 (MQ=255)

     ggTAACAGAGAAAGAGGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCtgtt                                                                                    >  1:3199202/1‑93 (MQ=255)

     ggTAACAGAGAAAGAGGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCtgtt                                                                                    <  1:2550448/93‑1 (MQ=255)

      gTAACAGAGAAAGAGGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCtgttg                                                                                   >  1:2783250/1‑93 (MQ=255)

       tAACAGAGAAAGAGGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCtgttgt                                                                                  >  2:4850241/1‑93 (MQ=255)

       tAACAGAGAAAGAGGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCtgttgt                                                                                  <  1:2256630/93‑1 (MQ=255)

        aaCAGAGAAAGAGGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCGGACGCTGAATACGATTACCGTTCTGTTGTc                                                                                 <  2:3809006/93‑1 (MQ=255)

          cAGAGAAAGAGGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGCATACGATGAGCGTTCTGATGTCGa                                                                               >  2:877532/1‑93 (MQ=255)

           agagAAAGAGGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGAt                                                                              <  1:3874370/93‑1 (MQ=255)

            gagaAAGAGGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGAtt                                                                             >  2:5026583/1‑93 (MQ=255)

            gagaAAGAGGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGAtt                                                                             >  1:6048805/1‑93 (MQ=255)

            gagaAAGAGGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGAtt                                                                             <  2:4296239/93‑1 (MQ=255)

            gagaAAGAGGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGAtt                                                                             >  2:148939/1‑93 (MQ=255)

             agaAAGAGGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTg                                                                            >  2:3399/1‑93 (MQ=255)

             agaAAGAGGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTg                                                                            >  2:2226842/1‑93 (MQ=255)

               aaaGAGGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGcc                                                                          <  1:4372376/93‑1 (MQ=255)

               aaaGAGGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGcc                                                                          <  1:5256702/93‑1 (MQ=255)

               aaaGAGGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGcc                                                                          <  2:2163712/93‑1 (MQ=255)

                aaGAGGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCcg                                                                         <  2:3538418/93‑1 (MQ=255)

                aaGAGGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCcg                                                                         <  2:5696784/93‑1 (MQ=255)

                 agagGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCcgc                                                                        <  2:2890651/93‑1 (MQ=255)

                 agagGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCcgc                                                                        <  2:544807/93‑1 (MQ=255)

                  gagGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCcgcg                                                                       >  1:1450483/1‑93 (MQ=255)

                   agGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCgcgc                                                                      <  2:4000485/93‑1 (MQ=255)

                     gcgcgcgcgcTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTa                                                                    <  2:5569685/93‑1 (MQ=255)

                        cgcgcgcTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAgcg                                                                 >  1:1932806/1‑93 (MQ=255)

                           gcgcTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTg                                                              >  1:5924165/1‑93 (MQ=255)

                            cgcTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTgg                                                             >  1:5807912/1‑93 (MQ=255)

                            cgcTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTgg                                                             >  1:3712062/1‑93 (MQ=255)

                            cgcTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTgg                                                             >  1:461322/1‑93 (MQ=255)

                            cgcTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTgg                                                             <  1:6157936/93‑1 (MQ=255)

                            cgcTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTgg                                                             <  1:974573/93‑1 (MQ=255)

                             gcTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGc                                                            >  2:4968533/1‑93 (MQ=255)

                               tGTTTTTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGt                                                          <  2:5904157/93‑1 (MQ=255)

                                gTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTg                                                         <  2:3067366/93‑1 (MQ=255)

                                gTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTg                                                         >  2:2702063/1‑93 (MQ=255)

                                gTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTg                                                         >  1:4019804/1‑93 (MQ=255)

                                gTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTg                                                         >  1:1333657/1‑93 (MQ=255)

                                gTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTg                                                         >  1:3803046/1‑93 (MQ=255)

                                gTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTg                                                         <  2:5315009/93‑1 (MQ=255)

                                 tttGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTgg                                                        >  1:2401101/1‑93 (MQ=255)

                                    gTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGgtg                                                     <  2:3240697/93‑1 (MQ=255)

                                     tCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGgtgt                                                    >  2:1991062/1‑93 (MQ=255)

                                       gTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTAc                                                  >  1:5739377/1‑93 (MQ=255)

                                        tGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTAcc                                                 <  2:716429/93‑1 (MQ=255)

                                        tGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTAcc                                                 >  2:6045544/1‑93 (MQ=255)

                                        tGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTAcc                                                 >  1:3153068/1‑93 (MQ=255)

                                         gCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTAccc                                                <  2:3584056/93‑1 (MQ=255)

                                         gCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTAccc                                                <  1:4527967/93‑1 (MQ=255)

                                           tGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGt                                              >  2:4185812/1‑93 (MQ=255)

                                              tACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGtttt                                           >  1:5369272/1‑92 (MQ=255)

                                              tACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTa                                           >  1:4921932/1‑93 (MQ=255)

                                               tcTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTAt                                          <  1:1709460/92‑1 (MQ=255)

                                               aCTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTAt                                          <  1:965124/93‑1 (MQ=255)

                                               aCTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTAt                                          >  1:1514403/1‑93 (MQ=255)

                                               aCTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTAt                                          >  1:2205500/1‑93 (MQ=255)

                                               aCTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTAt                                          >  1:1201450/1‑93 (MQ=255)

                                                cTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATg                                         >  1:3507441/1‑93 (MQ=255)

                                                 tGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGa                                        <  1:109379/93‑1 (MQ=255)

                                                 tGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGa                                        >  2:5182423/1‑93 (MQ=255)

                                                 tGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGa                                        >  1:4103804/1‑93 (MQ=255)

                                                 tGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGa                                        >  1:4983382/1‑93 (MQ=255)

                                                  gATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGaa                                       <  2:2438927/93‑1 (MQ=255)

                                                   aTTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAg                                      >  2:639587/1‑93 (MQ=255)

                                                    tttCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGc                                     >  2:5638348/1‑93 (MQ=255)

                                                    tttCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGc                                     <  2:124783/93‑1 (MQ=255)

                                                    tttCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGc                                     >  2:2410252/1‑93 (MQ=255)

                                                    tttCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGc                                     <  1:1679916/93‑1 (MQ=255)

                                                      tCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCgt                                   >  1:5648275/1‑92 (MQ=255)

                                                      tCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCgg                                   >  2:2881927/1‑93 (MQ=255)

                                                      tCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCgg                                   >  1:6212843/1‑93 (MQ=255)

                                                      tCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCgg                                   <  1:2169243/93‑1 (MQ=255)

                                                       cGTTTTTACTGGTGCTGACGCTGAATCCGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGt                                  <  2:5091324/93‑1 (MQ=255)

                                                        gTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGta                                 <  2:4578471/93‑1 (MQ=255)

                                                         tttttACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGtat                                >  2:5935390/1‑93 (MQ=255)

                                                            ttACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATAcc                             <  2:123643/93‑1 (MQ=255)

                                                              aCTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCa                           >  2:1731948/1‑93 (MQ=255)

                                                               cTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCAt                          >  1:4650962/1‑93 (MQ=255)

                                                                tGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATc                         >  2:1632210/1‑93 (MQ=255)

                                                                tGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATc                         >  1:1816736/1‑93 (MQ=255)

                                                                tGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATc                         <  2:3893598/93‑1 (MQ=255)

                                                                   tGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTAc                      >  1:1867798/1‑93 (MQ=255)

                                                                    gCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTAcc                     >  1:30453/1‑93 (MQ=255)

                                                                      tGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGGATACCCATCTACCGc                   >  2:385744/1‑93 (MQ=255)

                                                                        aCGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTACCGCaa                 >  2:2761965/1‑93 (MQ=255)

                                                                         cGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTACCGCAAg                >  1:5103473/1‑93 (MQ=255)

                                                                          gCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTACCGCAAgt               >  2:2579492/1‑93 (MQ=255)

                                                                          gCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAACCGGTATACCCATCTACCGCAAgg               >  2:5764336/1‑92 (MQ=255)

                                                                           cTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTACCGCAAgtg              >  1:1015463/1‑93 (MQ=255)

                                                                               aTACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTACCGCAAGTGGTGc          <  2:6207812/93‑1 (MQ=255)

                                                                                 aCGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTACCGCAAGTGGTGCTg        >  1:4791999/1‑93 (MQ=255)

                                                                                   gATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTACCGCAAGTGGTGCTggg      <  1:873274/93‑1 (MQ=255)

                                                                                   gATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTACCGCAAGTGGTGCTggg      <  1:1479607/93‑1 (MQ=255)

                                                                                    aTGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTACCGCAAGTGGTGCTGGgc     >  1:2949818/1‑93 (MQ=255)

                                                                                    aTGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTACCGCAAGTGGTGCTGGgc     >  2:4373320/1‑93 (MQ=255)

                                                                                      gACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTACCGCAAGTGGTGCTGGgcgc   >  1:2850991/1‑93 (MQ=255)

GGCGCGGTAACAGAGAAAGAGGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCATTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTACCGCAAGTGGTGCTGGGCGCG  >  NC_000913/4250859‑4251038

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

CGGCGCGGTAACAGAGAAAGAGGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCATTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTACCGCAAGTGGTGCTGGGCGCGGCG  >  NC_000913/4250858‑4251041

CGGCGCGGTAACAGAGAAAGAGGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTT                                                                                            >  SRR4302533.2736273/1‑94 (MQ=60)

  GCGCGGTAACAGAGAAAGAGGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCT                                                                                          <  SRR4302533.911827/94‑1 (MQ=60)

  GCGCGGTAACAGAGAAAGAGGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCT                                                                                          <  SRR4302533.6110421/94‑1 (MQ=60)

  GCGCGGTAACAGAGAAAGAGGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCT                                                                                          <  SRR4302533.3640418/94‑1 (MQ=60)

  GCGCGGTAACAGAGAAAGAGACGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCT                                                                                          <  SRR4302533.4229590/94‑1 (MQ=60)

   CGCGGTAACAGAGAAAGAGGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTG                                                                                         >  SRR4302533.1609868/1‑94 (MQ=60)

    GCGGTAACAGAGAAAGAGGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGT                                                                                        >  SRR4302533.437340/1‑94 (MQ=60)

    GCGGTAACAGAGAAAGAGGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGT                                                                                        >  SRR4302533.411499/1‑94 (MQ=60)

     CGGTAACAGAGAAAGAGGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTT                                                                                       >  SRR4302533.5522476/1‑94 (MQ=60)

     CGGTAACAGAGAAAGAGGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTT                                                                                       >  SRR4302533.3218889/1‑94 (MQ=60)

      GGTAACAGAGAAAGAGGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTG                                                                                      >  SRR4302533.1091178/1‑94 (MQ=60)

      GGTAACAGAGAAAGAGGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTG                                                                                      >  SRR4302533.2800347/1‑94 (MQ=60)

      GGTAACAGAGAAAGAGGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTG                                                                                      <  SRR4302533.2566144/94‑1 (MQ=60)

      GGTAACAGAGAAAGAGGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTG                                                                                      <  SRR4302533.1734686/94‑1 (MQ=60)

       GTAACAGAGAAAGAGGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGT                                                                                     <  SRR4302533.1517057/94‑1 (MQ=60)

        TAACAGAGAAAGAGGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTC                                                                                    <  SRR4302533.2270594/94‑1 (MQ=60)

            AGAGAAAGAGGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATT                                                                                >  SRR4302533.6085451/1‑94 (MQ=60)

            AGAGAAAGAGGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATT                                                                                <  SRR4302533.601897/94‑1 (MQ=60)

            AGAGAAAGAGGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATT                                                                                <  SRR4302533.3898022/94‑1 (MQ=60)

              AGAAAGAGGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGC                                                                              >  SRR4302533.4678036/1‑94 (MQ=60)

                AAAGAGGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCG                                                                            >  SRR4302533.3080010/1‑94 (MQ=60)

                AAAGAGGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCG                                                                            <  SRR4302533.211033/94‑1 (MQ=60)

                AAAGAGGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCG                                                                            <  SRR4302533.5288273/94‑1 (MQ=60)

                AAAGAGGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCG                                                                            <  SRR4302533.4398767/94‑1 (MQ=60)

                 AAGAGGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGC                                                                           >  SRR4302533.5320806/1‑94 (MQ=60)

                 AAGAGGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGC                                                                           <  SRR4302533.402768/94‑1 (MQ=60)

                  AGAGGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCG                                                                          <  SRR4302533.24513/94‑1 (MQ=60)

                  AGAGGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCG                                                                          <  SRR4302533.2242955/94‑1 (MQ=60)

                  AGAGGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCG                                                                          >  SRR4302533.1459235/1‑94 (MQ=60)

                   GAGGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGC                                                                         >  SRR4302533.2515182/1‑94 (MQ=60)

                    AGGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCT                                                                        >  SRR4302533.2866682/1‑94 (MQ=60)

                      GCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAG                                                                      >  SRR4302533.207167/1‑94 (MQ=60)

                       CGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGC                                                                     >  SRR4302533.6044939/1‑94 (MQ=60)

                       CGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGC                                                                     <  SRR4302533.2897071/94‑1 (MQ=60)

                        GCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCG                                                                    >  SRR4302533.1944596/1‑94 (MQ=60)

                        GCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCG                                                                    <  SRR4302533.1468015/94‑1 (MQ=60)

                          GCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCT                                                                  >  SRR4302533.493675/1‑94 (MQ=60)

                           CGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTG                                                                 >  SRR4302533.1910782/1‑94 (MQ=60)

                           CGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTG                                                                 >  SRR4302533.5959974/1‑94 (MQ=60)

                            GCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGG                                                                >  SRR4302533.5842984/1‑94 (MQ=60)

                            GCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGG                                                                >  SRR4302533.3734858/1‑94 (MQ=60)

                            GCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGG                                                                >  SRR4302533.3367359/1‑94 (MQ=60)

                            GCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGG                                                                >  SRR4302533.3299215/1‑94 (MQ=60)

                            GCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGG                                                                >  SRR4302533.464073/1‑94 (MQ=60)

                             CGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGC                                                               >  SRR4302533.4099468/1‑94 (MQ=60)

                             CGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGC                                                               <  SRR4302533.980294/94‑1 (MQ=60)

                             CGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGC                                                               <  SRR4302533.6194976/94‑1 (MQ=60)

                             CGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGC                                                               >  SRR4302533.2193811/1‑94 (MQ=60)

                             CGCTGTTTGTCGTGCTGGGACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGC                                                               >  SRR4302533.5708109/1‑94 (MQ=60)

                              GCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCG                                                              >  SRR4302533.2715572/1‑94 (MQ=60)

                               CTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGT                                                             <  SRR4302533.1637980/94‑1 (MQ=60)

                               CTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGT                                                             >  SRR4302533.1697882/1‑94 (MQ=60)

                               CTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGT                                                             >  SRR4302533.5453032/1‑94 (MQ=60)

                               CTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGG                                                             >  SRR4302533.3640753/1‑94 (MQ=60)

                               CTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGAAGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGG                                                             >  SRR4302533.4459478/1‑94 (MQ=60)

                                TGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTG                                                            >  SRR4302533.3826315/1‑94 (MQ=60)

                                TGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTG                                                            >  SRR4302533.4044282/1‑94 (MQ=60)

                                TGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTG                                                            <  SRR4302533.1293541/94‑1 (MQ=60)

                                TGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTG                                                            >  SRR4302533.6165728/1‑94 (MQ=60)

                                TGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTG                                                            >  SRR4302533.1341662/1‑94 (MQ=60)

                                 GTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGG                                                           >  SRR4302533.2415930/1‑94 (MQ=60)

                                   TTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGGTGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGT                                                         <  SRR4302533.4466029/94‑1 (MQ=60)

                                    TGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTG                                                        >  SRR4302533.5801078/1‑94 (MQ=60)

                                      TCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTA                                                      >  SRR4302533.1205982/1‑94 (MQ=60)

                                       CGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTAC                                                     >  SRR4302533.5774013/1‑94 (MQ=60)

                                        GTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACC                                                    <  SRR4302533.3124788/94‑1 (MQ=60)

                                        GTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACC                                                    >  SRR4302533.3172450/1‑94 (MQ=60)

                                          GCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCG                                                  <  SRR4302533.4555388/94‑1 (MQ=60)

                                           CTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGT                                                 <  SRR4302533.223066/94‑1 (MQ=60)

                                           CTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGT                                                 >  SRR4302533.5498277/1‑94 (MQ=60)

                                           CTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGT                                                 >  SRR4302533.2308947/1‑94 (MQ=60)

                                             GGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTT                                               <  SRR4302533.5125656/94‑1 (MQ=60)

                                              GTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTT                                              >  SRR4302533.5401525/1‑94 (MQ=60)

                                              GTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTA                                              >  SRR4302533.4951746/1‑94 (MQ=60)

                                               TACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTAT                                             >  SRR4302533.1208667/1‑94 (MQ=60)

                                               TACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTAT                                             >  SRR4302533.1523526/1‑94 (MQ=60)

                                               TACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTAT                                             >  SRR4302533.2219101/1‑94 (MQ=60)

                                                tcgGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTGTG                                            <  SRR4302533.3319683/91‑1 (MQ=60)

                                                TCTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATG                                            <  SRR4302533.1719820/94‑1 (MQ=60)

                                                TCTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATG                                            <  SRR4302533.4839599/94‑1 (MQ=60)

                                                ACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATG                                            <  SRR4302533.1380219/94‑1 (MQ=60)

                                                ACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATG                                            <  SRR4302533.970788/94‑1 (MQ=60)

                                                ACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATG                                            >  SRR4302533.3529071/1‑94 (MQ=60)

                                                 CTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGA                                           >  SRR4302533.1477394/1‑94 (MQ=60)

                                                 CTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGA                                           >  SRR4302533.5013599/1‑94 (MQ=60)

                                                 CTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGA                                           >  SRR4302533.4128768/1‑94 (MQ=60)

                                                 CTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGA                                           <  SRR4302533.2921613/94‑1 (MQ=60)

                                                 CTGATTTCG‑TTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAA                                          >  SRR4302533.1794244/1‑94 (MQ=60)

                                                  TGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAA                                          <  SRR4302533.5237530/94‑1 (MQ=60)

                                                  TGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAA                                          >  SRR4302533.3809055/1‑94 (MQ=60)

                                                  TGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAA                                          <  SRR4302533.110074/94‑1 (MQ=60)

                                                   GATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAG                                         >  SRR4302533.1919829/1‑94 (MQ=60)

                                                   GATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAG                                         >  SRR4302533.1566915/1‑94 (MQ=60)

                                                   GATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAG                                         >  SRR4302533.4802121/1‑94 (MQ=60)

                                                    ATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGC                                        <  SRR4302533.589190/94‑1 (MQ=60)

                                                     TTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCG                                       <  SRR4302533.1690093/94‑1 (MQ=60)

                                                      TTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGT                                      >  SRR4302533.5682422/1‑94 (MQ=60)

                                                      TTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGG                                      >  SRR4302533.6250096/1‑94 (MQ=60)

                                                       TCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGT                                     <  SRR4302533.2182585/94‑1 (MQ=60)

                                                         GTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTAT                                   <  SRR4302533.5649251/94‑1 (MQ=60)

                                                         GTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTAT                                   >  SRR4302533.5092741/1‑94 (MQ=60)

                                                         GTNTNTNCNGGTGCTGACGCTGNATACGATGACCGTTCTNTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTAT                                   <  SRR4302533.1178846/94‑1 (MQ=60)

                                                           TTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATAC                                 <  SRR4302533.4583353/94‑1 (MQ=60)

                                                            TTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACC                                >  SRR4302533.3175375/1‑94 (MQ=60)

                                                               ACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCAT                             >  SRR4302533.4679141/1‑94 (MQ=60)

                                                                CTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATC                            >  SRR4302533.1827825/1‑94 (MQ=60)

                                                                 TGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCT                           <  SRR4302533.2757291/94‑1 (MQ=60)

                                                                   GTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTAC                         >  SRR4302533.1879185/1‑94 (MQ=60)

                                                                   GTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTAC                         >  SRR4302533.6238215/1‑94 (MQ=60)

                                                                    TGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTACC                        >  SRR4302533.5558978/1‑94 (MQ=60)

                                                                    TGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTACC                        <  SRR4302533.1844546/94‑1 (MQ=60)

                                                                    TGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTACC                        >  SRR4302533.30659/1‑94 (MQ=60)

                                                                     GCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTACCG                       <  SRR4302533.155025/94‑1 (MQ=60)

                                                                       TGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTACCGCA                     >  SRR4302533.913331/1‑94 (MQ=60)

                                                                       TGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTACCGCA                     <  SRR4302533.2712766/94‑1 (MQ=60)

                                                                         ACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTACCGCAAG                   >  SRR4302533.5134302/1‑94 (MQ=60)

                                                                         ACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTACCGCAAG                   <  SRR4302533.723366/94‑1 (MQ=60)

                                                                           GCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTCCCCGTTTATGAAGCGGTATACCCATCTACCGCAAGTG                 <  SRR4302533.1450950/94‑1 (MQ=60)

                                                                           GCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTACCGCAAGTG                 >  SRR4302533.972309/1‑94 (MQ=60)

                                                                           GCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTACCGCAAGTG                 >  SRR4302533.1021429/1‑94 (MQ=60)

                                                                            CTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTACCGCAAGTGG                >  SRR4302533.1106555/1‑94 (MQ=60)

                                                                             TGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTACCGCAAGTGGT               <  SRR4302533.5217177/94‑1 (MQ=60)

                                                                             TGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTACCGCAAGTGGT               <  SRR4302533.2814809/94‑1 (MQ=60)

                                                                             TGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGGCGGTGTACCCGGTTATGAAGCGGGAGACCCATCTACCGCAGGTGGT               >  SRR4302533.1385914/1‑94 (MQ=60)

                                                                              GAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTACCGCAAGTGGTG              <  SRR4302533.817429/94‑1 (MQ=60)

                                                                              GAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTACCGCAAGTGGTG              >  SRR4302533.4630751/1‑94 (MQ=60)

                                                                              GAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTACCGCAAGTGGTG              <  SRR4302533.386962/94‑1 (MQ=60)

                                                                              GAATACGATGACCGTTCTGTTGTCGATTGCCGCGCGAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTACCGCAAGTGGTG              <  SRR4302533.1706428/94‑1 (MQ=60)

                                                                                 TACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTACCGCAAGTGGTGCTG           >  SRR4302533.4821035/1‑94 (MQ=60)

                                                                                    GATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTACCGCAAGTGGTGCTGGGC        >  SRR4302533.297933/1‑94 (MQ=60)

                                                                                    GATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTACCGCAAGTGGTGCTGGGC        <  SRR4302533.878402/94‑1 (MQ=60)

                                                                                    GATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTACCGCAAGTGGTGCTGGGC        >  SRR4302533.2967857/1‑94 (MQ=60)

                                                                                    GATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTACCGCAAGTGGTGCTGGGC        <  SRR4302533.1488543/94‑1 (MQ=60)

                                                                                    GATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTACCGCAAGTGGTGCTGGGC        >  SRR4302533.3873674/1‑94 (MQ=60)

                                                                                    GATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTACCGCAAGTGGTGCTGGGC        >  SRR4302533.4651263/1‑94 (MQ=60)

                                                                                     ATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTACCGCAAGTGGTGCTGGGCG       <  SRR4302533.5003605/94‑1 (MQ=60)

                                                                                      TGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTACCGCAAGTGGTGCTGGGCGC      >  SRR4302533.2868533/1‑94 (MQ=60)

                                                                                       GACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTACCGCAAGTGGTGCTGGGCGCG     >  SRR4302533.5798680/1‑94 (MQ=60)

                                                                                        ACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTACCGCAAGTGGTGCTGGGCGCGG    >  SRR4302533.2277380/1‑94 (MQ=60)

                                                                                        ACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTACCGCAAGTGGTGCTGGGCGCGG    >  SRR4302533.1721501/1‑94 (MQ=60)

                                                                                         CCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTACCGCAAGTGGTGCTGGGCGCGGC   >  SRR4302533.3541933/1‑94 (MQ=60)

                                                                                         CCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTACCGCAAGTGGTGCTGGGCGCGGC   <  SRR4302533.5937669/94‑1 (MQ=60)

                                                                                          CGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATATCCATCTACCGCAAGTGGTGCTGGGCGCGGCG  >  SRR4302533.5152051/1‑94 (MQ=60)

                                                                                          CGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTACCGCAAGTGGTGCTGGGCGCGGCG  >  SRR4302533.5538194/1‑94 (MQ=60)

CGGCGCGGTAACAGAGAAAGAGGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCATTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTACCGCAAGTGGTGCTGGGCGCGGCG  >  NC_000913/4250858‑4251041

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 7 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`