Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1624	250.6	12620048	98.0%	12367647	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,265,202	A→G	L170L (TTA→TTG)	tyrB →	tyrosine aminotransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,265,202	0	A	G	99.5%	700.0 / ‑4.9	218	L170L (TTA→TTG)	tyrB	tyrosine aminotransferase
Reads supporting (aligned to +/- strand): ref base A (1/0); new base G (122/94); total (123/95)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.28e-01

Only 100 of 227 total aligned reads displayed.

GGCTGGATTCGAAGTGAGTACTTACCCCTGGTATGACGAAGCGACTAACGGCGTGCGCTTTAATGACCTGTTGGCGACGCTGAAAACATTACCTGCCCGCAGTATTGTGTTGCTGCATCCATGTTGCCACAACCCAACGGGTGCCGATCTCACTAATGATCAGTGGGATGCGGTGATTGA  >  NC_000913/4265112‑4265291

  cTGGATTCGAAGTGAGTACTTACCCCTGGTATGACGAAGCGACTAACGGCGTGCGCTTTAATGACCTGTTGGCGACGCTGAAAACATTGCCTg                                                                                       >  1:4012651/1‑93 (MQ=255)

   tGGATTCGAAGTGAGTACTTACCCCTGGTATGACGAAGCGACTAACGGCGTGCGCTTTAATGACCTGTTGGCGACGCTGAAAACATTGCCTGc                                                                                      <  2:5485178/93‑1 (MQ=255)

    ggATTCGAAGTGAGTACTTACCCCTGGTATGACGAAGCGACTAACGGCGTGCGCTTTAATGACCTGTTGGCGACGCTGAAAACATtgcctgcc                                                                                     >  1:3453113/1‑93 (MQ=255)

    ggATTCGAAGTGAGTACTTACCCCTGGTATGACGAAGCGACTAACGGCGTGCGCTTTAATGACCTGTTGGCGACGCTGAAAACATtgcctgcc                                                                                     >  1:3253030/1‑93 (MQ=255)

    ggATTCGAAGTGAGTACTTACCCCTGGTATGACGAAGCGACTAACGGCGTGCGCTTTAATGACCTGTTGGCGACGCTGAAAACATtgcctgcc                                                                                     <  1:55188/93‑1 (MQ=255)

    ggATTCGAAGTGAGTACTTACCCCTGGTATGACGAAGCGACTAACGGCGTGCGCTTTAATGACCTGTTGGCGACGCTGAAAACATtgcctgcc                                                                                     <  2:2785585/93‑1 (MQ=255)

     gATTCGAAGTGAGTACTTACCCCTGGTATGACGAAGCGACTAACGGCGTGCGCTTTAATGACCTGTTGGCGACGCTGAAAACATTGCCTGccc                                                                                    >  1:1810206/1‑93 (MQ=255)

     gATTCGAAGTGAGTACTTACCCCTGGTATGACGAAGCGACTAACGGCGTGCGCTTTAATGACCTGTTGGCGACGCTGAAAACATTGCCTGccc                                                                                    >  2:448511/1‑93 (MQ=255)

       ttCGAAGTGAGTACTTACCCCTGGTATGACGAAGCGACTAACGGCGTGCGCTTTAATGACCTGTTGGCGACGCTGAAAACATTGCCTGCCCGc                                                                                  <  1:1710594/93‑1 (MQ=255)

        tCGAAGTGAGTACTTACCCCTGGTATGACGAAGCGACTAACGGCGTGCGCTTTAATGACCTGTTGGCGACGCTGAAAACATTGCCTGCCCGCa                                                                                 <  1:2315319/93‑1 (MQ=255)

         cGAAGTGAGTACTTACCCCTGGTATGACGAAGCGACTAACTGCGTGCGCTTTAATGACCTGTTGGCGACGCTGAAAACATTGCCTGCCCGCAg                                                                                >  1:2281362/1‑93 (MQ=255)

         cGAAGTGAGTACTTACCCCTGGTATGACGAAGCGACTAACGGCGTGCGCTTTAATGACCTGTTGGCGACGCTGAAAACATTGCCTGCCCGCAg                                                                                >  1:1671854/1‑93 (MQ=255)

         cGAAGTGAGTACTTACCCCTGGTATGACGAAGCGACTAACGGCGTGCGCTTTAATGACCTGTTGGCGACGCTGAAAACATTGCCTGCCCGCAg                                                                                >  2:3487740/1‑93 (MQ=255)

         cGAAGTGAGTACTTACCCCTGGTATGACGAAGCGACTAACGGCGTGCGCTTTAATGACCTGTTGGCGACGCTGAAAACATTGCCTGCCCGCAg                                                                                <  2:5562352/93‑1 (MQ=255)

          gAAGTGAGTACTTACCCCTGGTATGACGAAGCGACTAACGGCGTGCGCTTTAATGACCTGTTGGCGACGCTGAAAACATTGCCTGCCCGCAGt                                                                               <  2:4328813/93‑1 (MQ=255)

           aaGTGAGTACTTACCCCTGGTATGACGAAGCGACTAACGGCGTGCGCTTTAATGACCTGTTGGCGACGCTGAAAACATTGCCTGCCCGCAGTa                                                                              >  2:3392153/1‑93 (MQ=255)

           aaGTGAGTACTTACCCCTGGTATGACGAAGCGACTAACGGCGTGCGCTTTAATGACCTGTTGGCGACGCTGAAAACATTGCCTGCCCGCAGTa                                                                              <  2:1354135/93‑1 (MQ=255)

           aaGTGAGTACTTACCCCTGGTATGACGAAGCGACTAACGGCGTGCGCTTTAATGACCTGTTGGCGACGCTGAAAACATTGCCTGCCCGCAGTa                                                                              >  2:5966114/1‑93 (MQ=255)

           aaGTGAGTACTTACCCCTGGTATGACGAAGCGACTAACGGCGTGCGCTTTAATGACCTGTTGGCGACGCTGAAAACATTGCCTGCCCGCAGTa                                                                              <  2:2395931/93‑1 (MQ=255)

             gTGAGTACTTACCCCTGGTATGACGAAGCGACTAACGGCGTGCGCTTTAATGACCTGTTGGCGACGCTGAAAACATTGCCTGCCCGCAGTAtt                                                                            <  2:2121892/93‑1 (MQ=255)

               gAGTACTTACCCCTGGTATGACGAAGCGACTAACGGCGTGCGCTTTAATGACCTGTTGGCGACGCTGAAAACATTGCCTGCCCGCAGTATtgt                                                                          >  2:5710578/1‑93 (MQ=255)

                 gTACTTACCCCTGGTATGACGAAGCGACTAACGGCGTGCGCTTTAATGACCTGTTGGCGACGCTGAAAACATTGCCTGCCCGCAGTATTgtgt                                                                        >  1:5959650/1‑93 (MQ=255)

                  tACTTACCCCTGGTATGACGAAGCGACTAACGGCGTGCGCTTTAATGACCTGTTGGCGACGCTGAAAACATTGCCTGCCCGCAGTATTGTGtt                                                                       >  1:6012498/1‑93 (MQ=255)

                   aCTTACCCCTGGTATGACGAAGCGACTAACGGCGTGCGCTTTAATGACCTGTTGGCGACGCTGAAAACATTGCCTGCCCGCAGTATTGTGTtg                                                                      >  2:6240205/1‑93 (MQ=255)

                    cTTACCCCTGGTATGACGAAGCGACTAACGGCGTGCGCTTTAATGACCTGTTGGCGACGCTGAAAACATTGCCTGCCCGCAGTATTGTGTtgc                                                                     <  1:123657/93‑1 (MQ=255)

                    cTTACCCCTGGTATGACGAAGCGACTAACGGCGTGCGCTTTAATGACCTGTTGGCGACGCTGAAAAAATTGCCTGACCGCAATTTTGTGttcc                                                                     >  1:327614/1‑91 (MQ=255)

                     ttACCCCTGGTATGACGAAGCGACTAACGGCGTGCGCTTTAATGACCTGTTGGCGACGCTGAAAACATTGCCTGCCCGCAGTATTGTGTtgct                                                                    <  1:2865503/93‑1 (MQ=255)

                      tACCCCTGGTATGACGAAGCGACTAACGGCGTGCGCTTTAATGACCTGTTGGCGACGCTGAAAACATTGCCTGCCCGCAGTATTGTGTtgctg                                                                   >  2:497541/1‑93 (MQ=255)

                      tACCCCTGGTATGACGAAGCGACTAACGGCGTGCGCTTTAATGACCTGTTGGCGACGCTGAAAACATTGCCTGCCCGCAGTATTGTGTtgctg                                                                   >  1:187613/1‑93 (MQ=255)

                       aCCCCTGGTATGACGAAGCGACTAACGGCGTGCGCTTTAATGACCTGTTGGCGACGCTGAAAACATTGCCTGCCCGCAGTATTGTGTtgctgc                                                                  >  2:1307684/1‑93 (MQ=255)

                       aCCCCTGGTATGACGAAGCGACTAACGGCGTGCGCTTTAATGACCTGTTGGCGACGCTGAAAACATTGCCTGCCCGCAGTATTGTGTtgctgc                                                                  >  1:5391950/1‑93 (MQ=255)

                        ccccTGGTATGACGAAGCGACTAACGGCGTGCGCTTTAATGACCTGTTGGCGACGCTGAAAACATTGCCTGCCCGCAGTATTGTGTTGCTGCa                                                                 >  2:2500656/1‑93 (MQ=255)

                        ccccTGGTATGACGAAGCGACTAACGGCGTGCGCTTTAATGACCTGTTGGCGACGCTGAAAACATTGCCTGCCCGCAGTATTGTGTTGCTGCa                                                                 <  2:1174061/93‑1 (MQ=255)

                         cccTGGTATGACGAAGCGACTAACGGCGTGCGCTTTAATGACCTGTTGGCGACGCTGAAAACATTGCCTGCCCGCAGTATTGTGTTGCTGCAt                                                                >  1:2663211/1‑93 (MQ=255)

                         cccTGGTATGACGAAGCGACTAACGGCGTGCGCTTTAATGACCTGTTGGCGACGCTGAAAACATTGCCTGCCCGCAGTATTGTGTTGCTGCAt                                                                >  1:5631577/1‑93 (MQ=255)

                          ccTGGTATGACGAAGCGACTAACGGCGTGCGCTTTAATGACCTGTTGGCGACGCTGAAAACATTGCCTGCCCGCAGTATTGTGTTGCTGCATc                                                               >  1:76611/1‑93 (MQ=255)

                           cTGGTATGACGAAGCGACTAACGGCGTGCGCTTTAATGACCTGTTGGCGACGCTGAAAACATTGCCTGCCCGCAGTATTGTGTTGCTGCATcc                                                              >  2:3895151/1‑93 (MQ=255)

                           cTGGTATGACGAAGCGACTAACGGCGTGCGCTTTAATGACCTGTTGGCGACGCTGAAAACATTGCCTGCCCGCAGTATTGTGTTGCTGCATcc                                                              >  1:2975675/1‑93 (MQ=255)

                           cTGGTATGACGAAGCGACTAACGGCGTGCGCTTTAATGACCTGTTGGCGACGCTGAAAACATTGCCTGCCCGCAGTATTGTGTTGCTGCATcc                                                              >  1:5928867/1‑93 (MQ=255)

                           cTGGTATGACGAAGCGACTAACGGCGTGCGCTTTAATGACCTGTTGGCGACGCTGAAAACATTGCCTGCCCGCAGTATTGTGTTGCTGCATcc                                                              >  1:6254682/1‑93 (MQ=255)

                            tGGTATGACGAAGCGACTAACGGCGTGCGCTTTAATGACCTGTTGGCGACGCTGAAAACATTGCCTGCCCGCAGTATTGTGTTGCTGCATCCa                                                             >  1:4850961/1‑93 (MQ=255)

                            tGGTATGACGAAGCGACTAACGGCGTGCGCTTTAATGACCTGTTGGCGACGCTGAAAACATTGCCTGCCCGCAGTATTGTGTTGCTGCATCCa                                                             <  1:3397206/93‑1 (MQ=255)

                              gTATGACGAAGCGACTAACGGCGTGCGCTTTAATGACCTGTTGGCGACGCTGAAAACATTGCCTGCCCGCAGTATTGTGTTGCTGCATCCATg                                                           <  1:2251352/93‑1 (MQ=255)

                               tATGACGAAGCGACTAACGGCGTGCGCTTTAATGACCTGTTGGCGACGCTGAAAACATTGCCTGCCCGCAGTATTGTGTTGCTGCATCCATGt                                                          <  1:1539912/93‑1 (MQ=255)

                                 tGACGAAGCGACTAACGGCGTGCGCTTTAATGACCTGTTGGCGACGCTGAAAACATTGCCTGCCCGCANTATTGTGTTGCTGNATCCATGTTg                                                        >  2:3082702/1‑93 (MQ=255)

                                 tGACGAAGCGACTAACGGCGTGCGCTTTAATGACCTGTTGGCGACGCTGAAAACATTGCCTGCCCGCAGTATTGTGTTGCTGCATCCATGTTg                                                        >  1:4418435/1‑93 (MQ=255)

                                     gAAGCGACTAACGGCGTGCGCTTTAATGACCTGTTGGCGACGCTGAAAACATTGCCTGCCCGCAGTATTGTGTTGCTGCATCCATGTTGCcac                                                    >  2:477435/1‑93 (MQ=255)

                                           aCTAACGGCGTGCGCTTTAATGACCTGTTGGCGACGCTGAAAACATTGCCTGCCCGCAGTATTGTGTTGCTGCATCCATGTTGCCACAACCCa                                              >  1:6098085/1‑93 (MQ=255)

                                           aCTAACGGCGTGCGCTTTAATGACCTGTTGGCGACGCTGAAAACATTGCCTGCCCGCAGTATTGTGTTGCTGCATCCATGTTGCCACAACCCa                                              >  1:44792/1‑93 (MQ=255)

                                             tAACGGCGTGCGCTTTAATGACCTGTTGGCGACGCTGAAAACATTGCCTGCCCGCAGTATTGTGTTGCTGCATCCATGTTGCCACAACCCAAc                                            >  1:107527/1‑93 (MQ=255)

                                              aaCGGCGTGCGCTTTAATGACCTGTTGGCGACGCTGAAAACATTGCCTGCCCGCAGTATTGTGTTGCTGCATCCATGTTGCCACAACCCAACg                                           <  2:5827195/93‑1 (MQ=255)

                                              aaCGGCGTGCGCTTTAATGACCTGTTGGCGACGCTGAAAACATTGCCTGCCCGCAGTATTGTGTTGCTGCATCCATGTTGCCACAACCCAACg                                           <  1:3158684/93‑1 (MQ=255)

                                              aaCGGCGTGCGCTTTAATGACCTGTTGGCGACGCTGAAAACATTGCCTGCCCGCAGTATTGTGTTGCTGCATCCATGTTGCCACAACCCAACg                                           <  2:4171990/93‑1 (MQ=255)

                                                cGGCGTGCGCTTTAATGACCTGTTGGCGACGCTGAAAACATTGCCTGCCCGCAGTATTGTGTTGCTGCATCCATGTTGCCACAACCCAACggg                                         <  2:1404578/93‑1 (MQ=255)

                                                  gCGTGCGCTTTAATGACCTGTTGGCGACGCTGAAAACATTGCCTGCCCGCAGTATTGTGTTGCTGCATCCATGTTGCCACAACCCAACGGGTg                                       >  2:2857639/1‑93 (MQ=255)

                                                   cGTGCGCTTTAATGACCTGTTGGCGACGCTGAAAACATTTCCTGCCCGCAGTATTGTGTTGCTGCATCCAGGTTGCCACAACCCAACGGGTGc                                      <  2:1671843/93‑1 (MQ=255)

                                                    gTGCGCTTTAATGACCTGTTGGCGACGCTGAAAACATTGCCTGCCCGCAGTATTGTGTTGCTGCATCCATGTTGCCACAACCCAACGGGTGcc                                     >  2:4395529/1‑93 (MQ=255)

                                                     tGCGCTTTAATGACCTGTTGGCGACGCTGAAAACATTGCCTGCCCGCAGTATTGTGTTGCTGCATCCATGTTGCCACAACCCAACGGGTGCCg                                    >  2:176850/1‑93 (MQ=255)

                                                      gcgcTTTAATGACCTGTTGGCGACGCTGAAAACATTGCCTGCCCGCAGTATTGTGTTGCTGCATCCATGTTGCCACAACCCAACGGGTGCCGa                                   >  1:5898692/1‑93 (MQ=255)

                                                           ttAATGACCTGTTGGCGACGCTGAAAACATTGCCTGCCCGCAGTATTGTGTTGCTGCATCCATGTTGCCACAACCCAACGGGTGGCGATCTCa                              >  1:4314568/1‑93 (MQ=255)

                                                           ttAATGACCTGTTGGCGACGCTGAAAACATTGCCTGCCCGCAGTATTGTGTTGCTGCATCCATGTTGCCACAACCCAACGGGTGCCGATCTCa                              >  1:675481/1‑93 (MQ=255)

                                                            tAATGACCTGTTGGCGACGCTGAAAACATTGCCTGCCCGCAGTATTGTGTTGCTGCATCCATGTTGCCACAACCCAACGGGTGCCGATCTCAc                             >  1:2584890/1‑93 (MQ=255)

                                                                tacttGTTGGCGACTCTGAAAACATTGCCTGCCCGCAGTATTGTGTTGCTGCATCCATGTTGCCACAACCCAACGGGTGGCGATCTCACTAAt                         <  2:673820/89‑1 (MQ=255)

                                                                gACCTGTTGGCGACGCTGAAAACATTGCCTGCCCGCAGTATTGTGTTGCTGCATCCATGTTGCCACAACCCAACGGGTGCCGATCTCACTAAt                         >  2:5768173/1‑93 (MQ=255)

                                                                 aCCTGTTGGCGACGCTGAAAACATTGCCTGCCCGCAGTATTGTGTTGCTGCATCCATGTTGCCACAACCCAACGGGTGCCGATCTCACTAATg                        >  2:1805552/1‑93 (MQ=255)

                                                                 aCCTGTTGGCGACGCTGAAAACATTGCCTGCCCGCAGTATTGTGTTGCTGCATCCATGTTGCCACAACCCAACGGGTGCCGATCTCACTAATg                        <  1:1745367/93‑1 (MQ=255)

                                                                  ccTGTTGGCGACGCTGAAAACATTGCCTGCCCGCAGTATTGTGTTGCTGCATCCATGTTGCCACAACCCAACGGGTGCCGATCTCACTAATGa                       <  1:3738042/93‑1 (MQ=255)

                                                                  ccTGTTGGCGACGCTGAAAACATTGCCTGCCCGCAGTATTGTGTTGCTGCATCCATGTTGCCACAACCCAACGGGTGCCGATCTCACTAATGa                       <  2:4303906/93‑1 (MQ=255)

                                                                    tGTTGGCGACGCTGAAAACATTGCCTGCCCGCAGTATTGTGTTGCTGCATCCATGTTGCCACAACCCAACGGGTGCCGATCTCACTAATGATc                     <  1:4038032/93‑1 (MQ=255)

                                                                    tGTTGGCGACGCTGAAAACATTGCCTGCCCGCAGTATTGTGTTGCTGCATCCATGTTGCCACAACCCAACGGGTGCCGATCTCACTAATGATc                     >  2:1998974/1‑93 (MQ=255)

                                                                     gTTGGCGACGCTGAAAACATTGCCTGCCCGCAGTATTGTGTTGCTGCATCCATGTTGCCACAACCCAACGGGTGCCGATCTCACTAATGATCa                    <  2:4565049/93‑1 (MQ=255)

                                                                     gTTGGCGACGCTGAAAACATTGCCTGCCCGCAGTATTGTGTTGCTGCATCCATGTTGCCACAACCCAACGGGTGCCGATCTCACTAATGATCa                    <  1:5074272/93‑1 (MQ=255)

                                                                      ttGGCGACGCTGAAAACATTGCCTGCCCGCAGTATTGTGTTGCTGCATCCATGTTGCCACAACCCAATGGGTGCCGATCTCACTAATGATCAg                   <  2:4199099/93‑1 (MQ=255)

                                                                      ttGGCGACGCTGAAAACATTGCCTGCCCGCAGTATTGTGTTGCTGCATCCATGTTGCCACAACCCAACGGGTGCCGATCTCACTAATGATCAg                   <  1:3997171/93‑1 (MQ=255)

                                                                        ggCGACGCTGAAAACATTGCCTGCCCGCAGTATTGTGTTGCTGCATCCATGTTGCCACAACCCAACGGGTGCCGATCTCACTAATGATCAGTg                 <  1:2013834/93‑1 (MQ=255)

                                                                         gCGACGCTGAAAACATTGCCTGCCCGCAGTATTGTGTTGCTGCATCCATGTTGCCACAACCCAACGGGTGCCGATCTCACTAATGATCAGTgg                <  2:5215217/93‑1 (MQ=255)

                                                                         gCGACGCTGAAAACATTGCCTGCCCGCAGTATTGTGTTGCTGCATCCATGTTGCCACAACCCAACGGGTGCCGATCTCACTAATGATCAGTgg                <  1:4389188/93‑1 (MQ=255)

                                                                          cGACGCTGAAAACATTGCCTGCCCGCAGTATTGTGTTGCTGCATCCATGTTGCCACAACCCAACGGGTGCCGATCTCACTAATGATCAGTggg               >  2:3571145/1‑93 (MQ=255)

                                                                          cGACGCTGAAAACATTGCCTGCCCGCAGTATTGTGTTGCTGCATCCATGTTGCCACAACCCAACGGGTGCCGATCTCACTAATGATCAGTggg               <  1:1349588/93‑1 (MQ=255)

                                                                           gACGCTTAAAACATTGCCTGCCCGCAGTATTGTGTTGCTGCATCCATGTTGCCACAACCCAACGGGTGCCGATCTCACTAATGATCAGTGGGa              <  2:3321395/93‑1 (MQ=255)

                                                                           gACGCTGAAAACATTGCCTGCCCGCAGTATTGTGTTGCTGCATCCATGTTGCCACAACCCAACGGGTGCCGATCTCACTAATGATCAGTGGGa              >  2:2090952/1‑93 (MQ=255)

                                                                           gACGCTGAAAACATTGCCTGCCCGCAGTATTGTGTTGCTGCATCCATGTTGCCACAACCCAACGGGTGCCGATCTCACTAATGATCAGTGGGa              >  2:5265428/1‑93 (MQ=255)

                                                                            aCGCTGAAAACATTGCCTGCCCGCAGTATTGTGTTGCTGCATCCATGTTGCCACAACCCAACGGGTGCCGATCTCACTAATGATCAGTGGGAt             >  2:857174/1‑93 (MQ=255)

                                                                            aCGCTGAAAACATTGCCTGCCCGCAGTATTGTGTTGCTGCATCCATGTTGCCACAACCCAACGGGTGCCGATCTCACTAATGATCAGTGGGAt             >  1:4733599/1‑93 (MQ=255)

                                                                             cGCTGAAAACATTGCCTGCCCGCAGTATTGTGTTGCTGCATCCATGTTGCCACAACCCAACGGGTGCCGATCTCACTAATGATCAGTGGGATg            >  2:5170026/1‑93 (MQ=255)

                                                                              gCTGAAAACATTGCCTGCCCGCAGTATTGTGTTGCTGCATCCATGTTGCCACAACCCAACGGGTGCCGATCTCACTAATGATCAGTGGGATGc           >  1:3425742/1‑93 (MQ=255)

                                                                              gCTGAAAACATTGCCTGCCCGCAGTATTGTGTTGCTGCATCCATGTTGCCACAACCCAACGGGTGCCGATCTCACTAATGATCAGTGGGATGc           >  2:4596752/1‑93 (MQ=255)

                                                                               cTGAAAACATTGCCTGCCCGCAGTATTGTGTTGCTGCATCCATGTTGCCACAACCCAACGGGTGCCGATCTCACTAATGATCAGTGGGATGCg          <  1:1575615/93‑1 (MQ=255)

                                                                               cTGAAAACATTGCCTGCCCGCAGTATTGTGTTGCTGCATCCATGTTGCCACAACCCAACGGGTGCCGATCTCACTAATGATCAGTGGGATGCg          >  2:5456311/1‑93 (MQ=255)

                                                                                tGAAAACATTGCCTGCCCGCAGTATTGTGTTGCTGCATCCATGTTGCCACAACCCAACGGGTGCCGATCTCACTAATGATCAGTGGGATGCgg         >  1:929257/1‑93 (MQ=255)

                                                                                tGAAAACATTGCCTGCCCGCAGTATTGTGTTGCTGCATCCATGTTGCCACAACCCAACGGGTGCCGATCTCACTAATGATCAGTGGGATGCgg         >  1:5767059/1‑93 (MQ=255)

                                                                                 gAAAACATTGCCTGCCCGCAGTATTGTGTTGCTGCATCCATGTTGCCACAACCCAACGGGTGCCGATCTCACTAATGATCAGTGGGATGCggg        >  2:362847/1‑92 (MQ=255)

                                                                                 gAAAACATTGCCTGCCCGCAGTATTGTGTTGCTGCATCCATGTTGCCACAACCCAACGGGTGCCGATCTCACTAATGATCAGTGGGATGCGGt        >  1:838336/1‑93 (MQ=255)

                                                                                 gAAAACATTGCCTGCCCGCAGTATTGTGTTGCTGCATCCATGTTGCCACAACCCAACGGGTGCCGATCTCACTAATGATCAGTGGGATGCGGt        >  2:5915286/1‑93 (MQ=255)

                                                                                 gAAAACATTGCCTGCCCGCAGTATTGTGTTGCTGCATCCATGTTGCCACAACCCAACGGGTGCCGATCTCACTAATGATCAGTGGGATGCGGt        >  1:5466692/1‑93 (MQ=255)

                                                                                 gAAAACATTGCCTGCCCGCAGTATTGTGTTGCTGCATCCATGTTGCCACAACCCAACGGGTGCCGATCTCACTAATGATCAGTGGGATGCGGt        >  2:1847226/1‑93 (MQ=255)

                                                                                    aaCATTGCCTGCCCGCAGTATTGTGTTGCTGCATCCATGTTGCCACAACCCAACGGGTGCCGATCTCACTAATGATCAGTGGGATGCGGTGAt     >  1:4657017/1‑93 (MQ=255)

                                                                                    aaCATTGCCTGCCCGCAGTATTGTGTTGCTGCATCCATGTTGCCACAACCCAACGGGTGCCGATCTCACTAATGATCAGTGGGATGCGGTGAt     <  1:1956956/93‑1 (MQ=255)

                                                                                     aCATTGCCTGCCCGCAGTATTGTGTTGCTGCATCCATGTTGCCACAACCCAACGGGTGCCGATCTCACTAATGATCAGTGGGATGCGGTGAtt    <  2:2239578/93‑1 (MQ=255)

                                                                                     aCATTGCCTGCCCGCAGTATTGTGTTGCTGCATCCATGTTGCCACAACCCAACGGGTGCCGATCTCACTAATGATCAGTGGGATGCGGTGAtt    <  2:4107936/93‑1 (MQ=255)

GGCTGGATTCGAAGTGAGTACTTACCCCTGGTATGACGAAGCGACTAACGGCGTGCGCTTTAATGACCTGTTGGCGACGCTGAAAACATTACCTGCCCGCAGTATTGTGTTGCTGCATCCATGTTGCCACAACCCAACGGGTGCCGATCTCACTAATGATCAGTGGGATGCGGTGATTGA  >  NC_000913/4265112‑4265291

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

CGGGGCTGGATTCGAAGTGAGTACTTACCCCTGGTATGACGAAGCGACTAACGGCGTGCGCTTTAATGACCTGTTGGCGACGCTGAAAACATTACCTGCCCGCAGTATTGTGTTGCTGCATCCATGTTGCCACAACCCAACGGGTGCCGATCTCACTAATGATCAGTGGGATGCGGTGATTGAAATT  >  NC_000913/4265109‑4265295

CGGGGCTGGATTCGAAGTGAGTACTTACCCCTGGTATGACGAAGCGACTAACGGCGTGCGCTTTAATGACCTGTTGGCGACGCTGAAAACATTG                                                                                               >  SRR4302533.3901579/1‑94 (MQ=60)

CGGGGCTGGATTCGAAGTGAGTACTTACCCCTGGTATGACGAAGCGACTAACGGCGTGCGCTTTAATGACCTGTTGGCGACGCTGAAAACATTG                                                                                               <  SRR4302533.3995578/94‑1 (MQ=60)

CGGGGCTGGATTCGAAGTGAGTACTTACCCCTGGTATGACGAAGCGACTAACGGCGTGCGCTTTAATGACCTGTTGGCGACGCTGAAAACATTG                                                                                               <  SRR4302533.4932086/94‑1 (MQ=60)

CGGGGCTGGATTCGAAGTGAGTACTTACCCCTGGTATGACGAAGCGACTAACGGCGTGCGCTTTAATGACCTGTTGGCGACGCTGAAAACATTG                                                                                               >  SRR4302533.5514578/1‑94 (MQ=60)

CGGGGCTGGATTCGAAGTGAGTACTTACCCCTGGTATGACGAAGCGACTAACGGCGTGCGCTTTAATGACCTGTTGGCGACGCTGAAAACATTG                                                                                               <  SRR4302533.1792008/94‑1 (MQ=60)

   GGCTGGATTCGAAGTGAGTACTTACCCCTGGTATGACGAAGCGACTAACGGCGTGCGCTTTAATGACCTGTTGGCGACGCTGAAAACATTGCCT                                                                                            <  SRR4302533.3424237/94‑1 (MQ=60)

   GGCTGGATTCGAAGTGAGTACTTACCCCTGGTATGACGAAGCGACTAACGGCGTGCGCTTTAATGACCTGTTGGCGACGCTGAAAACATTGCCT                                                                                            <  SRR4302533.1293732/94‑1 (MQ=60)

   GGCTGGATTCGAAGTGAGTACTTACCCCTGGTATGACGAAGCGACTAACGGCGTGCGCTTTAATGACCTGTTGGCGACGCTGAAAACATTGCCT                                                                                            >  SRR4302533.594886/1‑94 (MQ=60)

    GCTGGATTCGAAGTGAGTACTTACCCCTGGTATGACGAAGCGACTAACGGCGTGCGCTTTAATGACCTGTTGGCGACGCTGAAAACATTGCCTG                                                                                           >  SRR4302533.4037083/1‑94 (MQ=60)

     CTGGATTCGAAGTGAGTACTTACCCCTGGTATGACGAAGCGACTAACGGCGTGCGCTTTAATGACCTGTTGGCGACGCTGAAAACATTGCCTGC                                                                                          >  SRR4302533.2893311/1‑94 (MQ=60)

     CTGGATTCGAAGTGAGTACTTACCCCTGGTATGACGAAGCGACTAACGGCGTGCGCTTTAATGACCTGTTGGCGACGCTGAAAACATTGCCTGC                                                                                          >  SRR4302533.1714936/1‑94 (MQ=60)

      TGGATTCGAAGTGAGTACTTACCCCTGGTATGACGAAGCGACTAACGGCGTGCGCTTTAATGACCTGTTGGCGACGCTGAAAACATTGCCTGCC                                                                                         >  SRR4302533.3273090/1‑94 (MQ=60)

      TGGATTCGAAGTGAGTACTTACCCCTGGTATGACGAAGCGACTAACGGCGTGCGCTTTAATGACCTGTTGGCGACGCTGAAAACATTGCCTGCC                                                                                         >  SRR4302533.3474432/1‑94 (MQ=60)

       GGATTCGAAGTGAGTACTTACCCCTGGTATGACGAAGCGACTAACGGCGTGCGCTTTAATGACCTGTTGGCGACGCTGAAAACATTGCCTGCCC                                                                                        >  SRR4302533.1821267/1‑94 (MQ=60)

       GGATTCGAAGTGAGTACTTACCCCTGGTATGACGAAGCGACTAACGGCGTGCGCTTTAATGACCTGTTGGCGACGCTGAAAACATTGCCTGCCC                                                                                        <  SRR4302533.55560/94‑1 (MQ=60)

        GATTCGAAGTGAGTACTTACCCCTGGTATGACGAAGCGACTAACGGCGTGCGCTTTAATGACCTGTTGGCGACGCTGAAAACATTGCCTGCCCG                                                                                       >  SRR4302533.1753874/1‑94 (MQ=60)

        GATTCGAAGTGAGTACTTACCCCTGGTATGACGAAGCGACTAACGGCGTGCGCTTTAATGACCTGTTGGCGACGCTGAAAACATTGCCTGCCCG                                                                                       <  SRR4302533.2545047/94‑1 (MQ=60)

          TTCGAAGTGAGTACTTACCCCTGGTATGACGAAGCGACTAACGGCGTGCGCTTTAATGACCTGTTGGCGACGCTGAAAACATTGCCTGCCCGCA                                                                                     <  SRR4302533.1720961/94‑1 (MQ=60)

          TTCGAAGTGAGTACTTACCCCTGGTATGACGAAGCGACTAACGGCGTGCGCTTTAATGACCTGTTGGCGACGCTGAAAACATTGCCTGCCCGCA                                                                                     <  SRR4302533.6330216/94‑1 (MQ=60)

           TCGAAGTGAGTACTTACCCCTGGTATGACGAAGCGACTAACTGCGTGCGCTTTAATGACCTGTTGGCGACGCTGAAAACATTGCCTGCCCGCAG                                                                                    >  SRR4302533.2295486/1‑94 (MQ=60)

           TCGAAGTGAGTACTTACCCCTGGTATGACGAAGCGACTAACGGCGTGCGCTTTAATGACCTGTTGGCGACGCTGAAAACATTGCCTGCCCGCAG                                                                                    >  SRR4302533.1681960/1‑94 (MQ=60)

           TCGAAGTGAGTACTTACCCCTGGTATGACGAAGCGACTAACGGCGTGCGCTTTAATGACCTGTTGGCGACGCTGAAAACATTGCCTGCCCGCAG                                                                                    <  SRR4302533.2329659/94‑1 (MQ=60)

             GAAGTGAGTACTTACCCCTGGTATGACGAAGCGACTAACGGCGTGCGCTTTAATGACCTGTTGTCGACGCTGAAAACATTGCCTGCCCGCAGTA                                                                                  >  SRR4302533.176735/1‑94 (MQ=60)

               AGTGAGTACTTACTCCTGGTATGACGAAGCGACTAACGGCGTGCGCTTTAATGACCTGTTGGCGACGCTGAAAACATTGCCTGCCCGCAGTATT                                                                                <  SRR4302533.2755858/94‑1 (MQ=60)

               AGTGAGTACTTACCCCTGGTATGACGAAGCGACTAACGGCGTGCGCTTTAATGACCTGTTGGCGACGCTGAAAACATTGCCTGCCCGCAGTATT                                                                                >  SRR4302533.2573100/1‑94 (MQ=60)

                GTGAGTACTTACCCCTGGTATGACGAAGCGACTAACGGCGTGCGCTTTAATGACCTGTTGGCGACGCTGAAAACATTGCCTGCCCGCAGTATTG                                                                               <  SRR4302533.6020736/94‑1 (MQ=60)

                   AGTACTTACCCCTGGTATGACGAAGCGACTAACGGCGTGCGCTTTAATGACCTGTTGGCGACGCTGAAAACATTGCCTGCCCGCAGTATTGTGT                                                                            >  SRR4302533.4481242/1‑94 (MQ=60)

                   AGTACTTACCCCTGGTATGACGAAGCGACTAACGGCGTGCGCTTTAATGACCTGTTGGCGACGCTGAAAACATTGCCTGCCCGCAGTATTGTGT                                                                            >  SRR4302533.5995700/1‑94 (MQ=60)

                    GTACTTCCCCCTGGTATGACGAAGCGACTAACGGCGTGCGCTTTAATGACCTGTTGGCGACGCTGAAAACATTGCCTGCCCGCAGTATTGTGTT                                                                           <  SRR4302533.2673571/94‑1 (MQ=60)

                    GTACTTACCCCTGGTATGACGAAGCGACTAACGGCGTGCGCTTTAATGACCTGTTGGCGACGCTGAAAACATTGCCTGCCCGCAGTATTGTGTT                                                                           >  SRR4302533.6048924/1‑94 (MQ=60)

                     TACTTACCCCTGGTATGACGAAGCGACTAACGGCGTGCGCTTTAATGACCTGTTGGCGACGCTGAAAACATTGCCTGCCCGCAGTATTGTGTTG                                                                          <  SRR4302533.4064064/94‑1 (MQ=60)

                      ACTTACCCCTGGTATGACGAAGCGACTAACGGCGTGCGCTTTAATGACCTGTTGGCGACGCTGAAAAAATTGCCTGACCGCAATTTTGTGTTCC                                                                         >  SRR4302533.329607/1‑94 (MQ=60)

                       CTTACCCCTGGTATGACGAAGCGACTAACGGCGTGCGCTTTAATGACCTGTTGGCGACGCTGAAAACATTGCCTGCCCGCAGTATTGTGTTGCT                                                                        <  SRR4302533.124434/94‑1 (MQ=60)

                       CTTACCCCTGGTATGACGAAGCGACTAACGGCGTGCGCTTTAATGACCTGTTGGCGACGCTGAAAACATTGCCTGCCCGCAGTATTGTGTTGCT                                                                        >  SRR4302533.4722663/1‑94 (MQ=60)

                       CTTACCCCTGGTATGACGAAGCGACTAACGGCGTGCGCTTTAATGACCTGTTGGCGACGCTGAAAACATTGCCTGCCCGCAGTATTGTGTTGCT                                                                        <  SRR4302533.3158423/94‑1 (MQ=60)

                        TTACCCCTGGTATGACGAAGCGACTAACGGCGTGCGCTTTAATGACCTGTTGGCGACGCTGAAAACATTGCCTGCCCGCAGTATTGTGTTGCTG                                                                       >  SRR4302533.188781/1‑94 (MQ=60)

                        TTACCCCTGGTATGACGAAGCGACTAACGGCGTGCGCTTTAATGACCTGTTGGCGACGCTGAAAACATTGCCTGCCCGCAGTATTGTGTTGCTG                                                                       <  SRR4302533.2883133/94‑1 (MQ=60)

                         TACCCCTGGTATGACGAAGCGACTAACGGCGTGCGCTTTAATGACCTGTTGGCGACGCTGAAAACATTGCCTGCCCGCAGTATTGTGTTGCTGC                                                                      >  SRR4302533.5424345/1‑94 (MQ=60)

                          ACCCCTGGTATGACGAAGCGACTAACGGCGTGCGCTTTAATGACCTGTTGGCGACGCTGAAAACATTGCCTGCCCGCAGTATTGTGTTGCTGCA                                                                     >  SRR4302533.1879377/1‑94 (MQ=60)

                           CCCCTGGTATGACGAAGCGACTAACGGCGTGCGCTTTAATGACCTGTTGGCGACGCTGAAAACATTGCCTGCCCGCAGTATTGTGTTGCTGCAT                                                                    >  SRR4302533.5665634/1‑94 (MQ=60)

                           CCCCTGGTATGACGAAGCGACTAACGGCGTGCGCTTTAATGACCTGTTGGCGACGCTGAAAACATTGCCTGCCCGCAGTATTGTGTTGCTGCAT                                                                    >  SRR4302533.2679561/1‑94 (MQ=60)

                            CCCTGGTATGACGAAGCGACTAACGGCGTGCGCTTTAATGACCTGTTGGCGACGCTGAAAACATTGCCTGCCCGCAGTATTGTGTTGCTGCATC                                                                   >  SRR4302533.77104/1‑94 (MQ=60)

                            CCCTGGTATGACGAAGCGACTAACGGCGTGCGCTTTAATGACCTGTTGGCGACGCTGAAAACATTGCCTGCCCGCAGTATTGTGTTGCTGCATC                                                                   <  SRR4302533.1559822/94‑1 (MQ=60)

                             CCTGGTATGACGAAGCGACTAACGGCGTGCGCTTTAATGACCTGTTGGCGACGCTGAAAACATTGCCTGCCCGCAGTATTGTGTTGCTGCATCC                                                                  >  SRR4302533.6292089/1‑94 (MQ=60)

                             CCTGGTATGACGAAGCGACTAACGGCGTGCGCTTTAATGACCTGTTGGCGACGCTGAAAACATTGCCTGCCCGCAGTATTGTGTTGCTGCATCC                                                                  >  SRR4302533.5964703/1‑94 (MQ=60)

                             CCTGGTATGACGAAGCGACTAACGGCGTGCGCTTTAATGACCTGTTGGCGACGCTGAAAACATTGCCTGCCCGCAGTATTGTGTTGCTGCATCC                                                                  >  SRR4302533.2993882/1‑94 (MQ=60)

                             CCTGGTATGACGAAGCGACTAACGGCGTGCGCTTTAATGACCTGTTGGCGACGCTGAAAACATTGCCTGCCCGCAGTATTGTGTTGCTGCATCC                                                                  >  SRR4302533.4549805/1‑94 (MQ=60)

                              CTGGTATGACGAAGCGACTAACGGCGTGCGCTTTAATGACCTGTTGGCGACGCTGAAAACATTGCCTGCCCGCAGTATTGTGTTGCTGCATCCA                                                                 <  SRR4302533.4050622/94‑1 (MQ=60)

                              CTGGTATGACGAAGCGACTAACGGCGTGCGCTTTAATGACCTGTTGGCGACGCTGAAAACATTGCCTGCCCGCAGTATTGTGTTGCTGCATCCA                                                                 >  SRR4302533.4880357/1‑94 (MQ=60)

                               TGGTATGACGAAGCGACTAACGGCGTGCGCTTTAATGACCTGTTGGCGACGCTGAAAACATTGCCTGCCCGCAGTATTGTGTTGCTGCATCCAT                                                                <  SRR4302533.3418185/94‑1 (MQ=60)

                               TGGTATGACGAAGCGACTAACGGCGTGCGCTTTAATGACCTGTTGGCGACGCTGAAAACATTGCCTGCCCGCAGTATTGTGTTGCTGCATCCAT                                                                <  SRR4302533.4849611/94‑1 (MQ=60)

                                GGTATGACGAAGCGACTAACGGCGTGCGCTTTAATGACCTGTTGGCGACGCTGAAAACATTGCCTGCCCGCAGTATTGTGTTGCTGCATCCATG                                                               <  SRR4302533.6048073/94‑1 (MQ=60)

                                 GTATGACGAAGCGACTAACGGCGTGCGCTTTAATGACCTGTTGGCGACGCTGAAAACATTGCCTGCCCGCAGTATTGTGTTGCTGCATCCATGT                                                              >  SRR4302533.5390019/1‑94 (MQ=60)

                                 GTATGACGAAGCGACTAACGGCGTGCGCTTTAATGACCTGTTGGCGACGCTGAAAACATTGCCTGCCCGCAGTATTGTGTTGCTGCATCCATGT                                                              <  SRR4302533.2265271/94‑1 (MQ=60)

                                  TATGACGAAGCGACTAACGGCGTGCGCTTTAATGACCTGTTGGCGACGCTGAAAACATTGCCTGCCCGCAGTATTGTGTTGCTGCATCCATGTT                                                             <  SRR4302533.1549186/94‑1 (MQ=60)

                                   ATGACGAAGCGACTAACGGCGTGCGCTTTAATGACCTGTTGGCGACGCTGAAAACATTGCCTGCCCGCAGTATTGTGTTGCTGCATCCATGTTG                                                            >  SRR4302533.4445149/1‑94 (MQ=60)

                                     GACGAAGCGACTAACGGCGTGCGCTTTAATGACCTGTTGGCGACGCTGAAAACATTGCCTGCCCGCAGTATTGTGTTGCTGCATCCATGTTGCC                                                          <  SRR4302533.6109607/94‑1 (MQ=60)

                                     GACGAAGCGACTAACGGCGTGCGCTTTAATGACCTGTTGGCGACGCTGAAAACATTGCCTGCCCGCAGTATTGTGTTGCTGCATCCATGTTGCC                                                          >  SRR4302533.4709193/1‑94 (MQ=60)

                                      ACGAAGCGACTAACGGCGTGCGCTTTAATGACCTGTTGGCGACGCTGAAAACATTGCCTGCCCGCAGTATTGTGTTGCTGCATCCATGTTGCCA                                                         <  SRR4302533.4985718/94‑1 (MQ=60)

                                       CGAAGCGACTAACGGCGTGCGCTTTAATGACCTGTTGGCGACGCTGAAAACATTGCCTGCCCGCAGTATTGTGTTGCTGCATCCATGTTGCCAC                                                        <  SRR4302533.1144033/94‑1 (MQ=60)

                                       CGAAGCGACTAACGGCGTGCGCTTTAATGACCTGTTGGCGACGCTGAAAACATTGCCTGCCCGCAGTATTGTGTTGCTGCATCCATGTTGCCAC                                                        >  SRR4302533.832894/1‑94 (MQ=60)

                                        GAAGCGACTAACGGCGTGCGCTTTAATGACCTGTTGGCGACGCTGAAAACATTGCCTGCCCGCAGTATTGTGTTGCTGCATCCATGTTGCCACA                                                       >  SRR4302533.2057518/1‑94 (MQ=60)

                                             GACTAACGGCGTGCGCTTTAATGACCTGTTGGCGACGCTGAAAACATTGCCTGCCCGCAGTATTGTGTTGCTGCATCCATGTTGCCACAACCCA                                                  <  SRR4302533.3217159/94‑1 (MQ=60)

                                             GACTAACGGCGTGCGCTTTAATGACCTGTTGGCGACGCTGAAAACATTGCCTGCCCGCAGTATTGTGTTGCTGCATCCATGTTGCCACAACCCA                                                  <  SRR4302533.5825048/94‑1 (MQ=60)

                                             GACTAACGGCGTGCGCTTTAATGACCTGTTGGCGACGCTGAAAACATTGCCTGCCCGCAGTATTGTGTTGCTGCATCCATGTTGCCACAACCCA                                                  >  SRR4302533.6134915/1‑94 (MQ=60)

                                             GACTAACGGCGTGCGCTTTAATGACCTGTTGGCGACGCTGAAAACATTGCCTGCCCGCAGTATTGTGTTGCTGCATCCATGTTGCCACAACCCA                                                  >  SRR4302533.45101/1‑94 (MQ=60)

                                               CTAACGGCGTGCGCTTTAATGACCTGTTGGCGACGCTGAAAACATTGCCTGCCCGCAGTATTGTGTTGCTGCATCCATGTTGCCACAACCCAAC                                                >  SRR4302533.108204/1‑94 (MQ=60)

                                               CTAACGGCGTGCGCTTTAATGACCTGTTGGCGACGCTGAAAACATTGCCTGCCCGCAGTATTGTGTTGCTGCATCCATGTTGCCACAACCCAAC                                                <  SRR4302533.879825/94‑1 (MQ=60)

                                                 AACGGCGTGCGCTTTAATGACCTGTTGGCGACGCTGAAAACATTGCCTGCCCGCAGTATTGTGTTGCTGCATCCATGTTGCCACAACCCAACGG                                              <  SRR4302533.1805183/94‑1 (MQ=60)

                                                 AACGGCGTGCGCTTTAATGACCTGTTGGCGACGCTGAAAACATTGCCTGCCCGCAGTATTGTGTTGCTGCATCCATGTTGCCACAACCCAACGG                                              <  SRR4302533.3178122/94‑1 (MQ=60)

                                                  ACGGCGTGCGCCTTAATGACCTGTTGGCGACGCTGAAAACATTGCCTGCCCGCAGTATTGTGTTGCTGCATCCATGTTGCCACAACCCAACGGG                                             <  SRR4302533.1563259/94‑1 (MQ=60)

                                                   CGGCGTGCGCTTTAATGACCTGTTGGCGACGCTGAAAACATTGCCTGCCCGCAGTATTGTGTTGCTGCATCCATGTTGCCACAACCCAACGGGT                                            <  SRR4302533.2303910/94‑1 (MQ=60)

                                                     GCGTGCGCTTTAATGACCTGTTGGCGACGCTGAAAACATTGCCTGCCCGCAGTATTGTGTTGCTGCATCCATGTTGCCACAACCCAACGGGTGC                                          >  SRR4302533.698359/1‑94 (MQ=60)

                                                       GTGCGCTTTAATGACCTGTTGGCGACGCTGAAAACATTGCCTGCCCGCAGTATTGTGTTGCTGCATCCATGTTGCCACAACCCAACGGGTGCCG                                        >  SRR4302533.3948137/1‑94 (MQ=60)

                                                       GTGCGCTTTAATGACCTGTTGGCGACGCTGAAAACATTGCCTGCCCGCAGTATTGTGTTGCTGCATCCATGTTGCCACAACCCAACGGGTGCCG                                        >  SRR4302533.1407420/1‑94 (MQ=60)

                                                        TGCGCTTTAATGACCTGTTGGCGACGCTGAAAACATTGCCTGCCCGCAGTATTGTGTTGCTGCATCCATGTTGCCACAACCCAACGGGTGCCGA                                       >  SRR4302533.5934316/1‑94 (MQ=60)

                                                           GCTTTAATGACCTGTTGGCGACGCTGAAAACATTACCTGCCCGCAGTATTGTGTTGCTGCATCCATGTTGCCACAACCCAACGGGTGCCGATCT                                    >  SRR4302533.3424462/1‑94 (MQ=60)

                                                            CTTTAATGACCTGTTGGCGACGCTGAAAACATTGCCTGCCCGCAGTATTGTGTTGCTGCATCCATGTTGCCACAACCCAACGGGTGCCGATCTC                                   >  SRR4302533.329577/1‑94 (MQ=60)

                                                             TTTAATGACCTGTTGGCGACGCTGAAAACATTGCCTGCCCGCAGTATTGTGTTGCTGCATCCATGTTGCCACAACCCAACGGGTGGCGATCTCA                                  >  SRR4302533.4340545/1‑94 (MQ=60)

                                                             TTTAATGACCTGTTGGCGACGCTGAAAACATTGCCTGCCCGCAGTATTGTGTTGCTGCATCCATGTTGCCACAACCCAACGGGTGCCGATCTCA                                  >  SRR4302533.679472/1‑94 (MQ=60)

                                                             TTTAATGACCTGTTGGCGACGCTGAAAACATTGCCTGCCCGCAGTATTGTGTTGCTGCATCCATGTTGCCACAACCCAACGGGTGCCGATCTCA                                  >  SRR4302533.5966288/1‑94 (MQ=60)

                                                              TTAATGACCTGTTGGCGACGCTGAAAACATTGCCTGCCCGCAGTATTGTGTTGCTGCATCCATGTTGCCACAACCCAACGGGTGCCGATCTCAC                                 <  SRR4302533.4378296/94‑1 (MQ=60)

                                                              TTAATGACCTGTTGGCGACGCTGAAAACATTGCCTGCCCGCAGTATTGTGTTGCTGCATCCATGTTGCCACAACCCAACGGGTGCCGATCTCAC                                 >  SRR4302533.5782345/1‑94 (MQ=60)

                                                              TTAATGACCTGTTGGCGACGCTGAAAACATTGCCTGCCCGCAGTATTGTGTTGCTGCATCCATGTTGCCACAACCCAACGGGTGCCGATCTCAC                                 >  SRR4302533.2600782/1‑94 (MQ=60)

                                                                 ATGACCTGTTGGCGACGCTGAAAACATTGCCTGCCCGCAGTATTGTGTTGCTGCATCCATGTTGCCACAACCCAACGGGTGCCGATCTCACTAA                              <  SRR4302533.3915781/94‑1 (MQ=60)

                                                                 ATGACCTGTTGGCGACGCTGAAAACATTGCCTGCCCGCAGTATTGTGTTGCTGCATCCATGTTGCCACAACCCAACGGGTGCCGATCTCACTAA                              <  SRR4302533.1962812/94‑1 (MQ=60)

                                                                  TGACCTGTTGGCGACGCTGAAAACATTGCCTGCCCGCAGTATTGTGTTGCTGCATCCATGTTGCCACAACCCAACGGGTGCCGATCTCACTAAT                             <  SRR4302533.3358576/94‑1 (MQ=60)

                                                                  TGACCTGTTGGCGACGCTGAAAACATTGCCTGCCCGCAGTATTGTGTTGCTGCATCCATGTTGCCACAACCCAACGGGTGCCGATCTCACTAAT                             >  SRR4302533.3448589/1‑94 (MQ=60)

                                                                   GACCTGTTGGCGACGCTGAAAACATTGCCTGCCCGCAGTATTGTGTTGCTGCATCCATGTTGCCACAACCCAACGGGTGCCGATCTCACTAATG                            >  SRR4302533.5657603/1‑94 (MQ=60)

                                                                    ACCTGTTGGCGACGCTGAAAACATTGCCTGCCCGCAGTATTGTGTTGCTGCATCCATGTTGCCACAACCCAACGGGTGCCGATCTCACTAATGA                           <  SRR4302533.1755984/94‑1 (MQ=60)

                                                                    ACCTGTTGGCGACGCTGAAAACATTGCCTGCCCGCAGTATTGTGTTGCTGCATCCATGTTGCCACAACCCAACGGGTGCCGATCTCACTAATGA                           <  SRR4302533.4384535/94‑1 (MQ=60)

                                                                     CCTGTTGGCGACGCTGAAAACATTGCCTGCCCGCAGTATTGTGTTGCTGCATCCATGTTGCCACAACCCAACGGGTGCCGATCTCACTAATGAT                          <  SRR4302533.3760969/94‑1 (MQ=60)

                                                                       TGTTGGCGACGCTGAAAACATTGCCTGCCCGCAGTATTGTGTTGCTGCATCCATGTTGCCACAACCCAACGGGTGCCGATCTCACTAATGATCA                        <  SRR4302533.4062603/94‑1 (MQ=60)

                                                                        GTTGGCGACGCTGAAAACATTGCCTGCCCGCAGTATTGTGTTGCTGCATCCATGTTGCCACACCCCAACGGGTGCCGATCTCACTAATGATCAG                       <  SRR4302533.5462790/94‑1 (MQ=60)

                                                                        GTTGGCGACGCTGAAAACATTGCCTGCCCGCAGTATTGTGTTGCTGCATCCATGTTGCCACAACCCAACGGGTGCCGATCTCACTAATGATCAG                       >  SRR4302533.2027540/1‑94 (MQ=60)

                                                                        GTTGGCGACGCTGAAAACATTGCCTGCCCGCAGTATTGTGTTGCTGCATCCATGTTGCCACAACCCAACGGGTGCCGATCTCACTAATGATCAG                       <  SRR4302533.5104945/94‑1 (MQ=60)

                                                                        GTTGGCGACGCTGAAAACATTGCCTGCCCACAGTATTGTGTTGCTGCATCCATGTTGCCACAACCCAACGGGTGCCGATCTCACTAATGATCAG                       >  SRR4302533.57000/1‑94 (MQ=60)

                                                                         TTGGCGACGCTGAAAACATTGCCTGCCCGCAGTATTGTGTTGCTGCATCCATGTTGCCACAACCCAACGGGTGCCGATCTCACTAATGATCAGT                      <  SRR4302533.4021511/94‑1 (MQ=60)

                                                                          TGGCGACGCTGAAAACATTGCCTGCCCGCAGTATTGTGTTGCTGCATCCATGTTGCCACAACCCAACGGGTGCCGATCTCACTAATGATCAGTG                     <  SRR4302533.6256884/94‑1 (MQ=60)

                                                                           GGCGACGCTGAAAACATTGCCTGCCCGCAGTATTGTGTTGCTGCATCCATGTTGCCACAACCCAACGGGTGCCGATCTCACTAATGATCAGTGG                    <  SRR4302533.2026241/94‑1 (MQ=60)

                                                                            GCGACGCTGAAAACATTGCCTGCCCGCAGTATTGTGTTGCTGCATCCATGTTGCCACAACCCAACGGGTGCCGATCTCACTAATGATCAGTGGG                   <  SRR4302533.4415688/94‑1 (MQ=60)

                                                                            GCGACGCTGAAAACATTGCCTGCCCGCAGTATTGTGTTGCTGCATCCATGTTGACACAACCAAACGGGTGCCGATCTCACTAATGATCAGTGGG                   <  SRR4302533.5467173/94‑1 (MQ=60)

                                                                             CGACGCTGAAAACATTGCCTGCCCGCAGTATTGTGTTGCTGCATCCATGTTGCCACAACCCAACGGGTGCCGATCTCACTAATGATCAGTGGGA                  <  SRR4302533.1357706/94‑1 (MQ=60)

                                                                              GACGCTGAAAACATTGCCTGCCCGCAGTATTGTGTTGCTGCATCCATGTTGCCACAACCCAACGGGTGCCGATCTCACTAATGATCAGTGGGAT                 >  SRR4302533.4762295/1‑94 (MQ=60)

                                                                                CGCTGAAAACATTGCCTGCCCGCAGTATTGTGTTGCTGCATCCATGTTGCCACAACCCAACGGGTGCCGATCTCACTAATGATCAGTGGGATGC               >  SRR4302533.3732662/1‑94 (MQ=60)

                                                                                CGCTGAAAACATTGCCTGCCCGCAGTATTGTGTTGCTGCATCCATGTTGCCACAACCCAACGGGTGCCGATCTCACTAATGATCAGTGGGATGC               >  SRR4302533.3446902/1‑94 (MQ=60)

                                                                                CGCTGAAAACATTGCCTGCCCGCAGTATTGTGTTGCTGCATCCATGTTGCCACAACCCAACGGGTGCCGATCTCACTAATGATCAGTGGGATGC               <  SRR4302533.4433031/94‑1 (MQ=60)

                                                                                  CTGAAAACATTGCCTGCCCGCAGTATTGTGTTGCTGCNTCCATGTTGCCACAACCCAACGGGTNCCGATCTCACNAATGANCAGTNGNANGNGG             >  SRR4302533.819705/1‑94 (MQ=60)

                                                                                  CTGAAAACATTGCCTGCCCGCAGTATTGTGTTGCTGCATCCATGTTGCCACAACCCAACGGGTGCCGATCTCACTAATGATCAGTGGGATGCGG             <  SRR4302533.1585101/94‑1 (MQ=60)

                                                                                  CTGAAAACATTGCCTGCCCGCAGTATTGTGTTGCTGCATCCATGTTGCCACAACCCAACGGGTGCCGATCTCACTAATGATCAGTGGGATGCGG             >  SRR4302533.934711/1‑94 (MQ=60)

                                                                                  CTGAAAACATTGCCTGCCCGCAGTATTGTGTTGCTGCATCCATGTTGCCACAACCCAACGGGTGCCGATCTCACTAATGATCAGTGGGATGCGG             >  SRR4302533.2268362/1‑94 (MQ=60)

                                                                                  CTGAAAACATTGCCTGCCCGCAGTATTGTGTTGCTGCATCCATGTTGCCACAACCCAACGGGTGCCGATCTCACTAATGATCAGTGGGATGCGG             >  SRR4302533.5801875/1‑94 (MQ=60)

                                                                                   TGAAAACATTGCCTGCCCGCAGTATTGTGTTGCTGCATCCATGTTGCCACAACCCAACGGGTGCCGATCTCACTAATGATCAGTGGGATGCGGT            >  SRR4302533.843245/1‑94 (MQ=60)

                                                                                   TGAAAACATTGCCTGCCCGCAGTATTGTGTTGCTGCATCCATGTTGCCACAACCCAACGGGTGCCGATCTCACTAATGATCAGTGGGATGCGGT            >  SRR4302533.5499643/1‑94 (MQ=60)

                                                                                   TGAAAACATTGCCTGCCCGCAGTATTGTGTTGCTGCATCCATGTTGCCACAACCCAACGGGTGCCGATCTCACTAATGATCAGTGGGATGCGGG            >  SRR4302533.201585/1‑94 (MQ=60)

                                                                                    GAAAACATTGCCTGCCCGCAGTATTGTGTTGCTGCATCCATGTTGCCACAACCCAACGGGTGCCGATCTCACTAATGATCAGTGGGATGCGGTG           >  SRR4302533.4028308/1‑94 (MQ=60)

                                                                                    GAAAACATTGCCTGCCCGCAGTATTGTGTTGCTGCATCCATGTTGCCACAACCCAACGGGTGCCGATCTCACTAATGATCAGTGGGATGCGGTG           <  SRR4302533.5547940/94‑1 (MQ=60)

                                                                                     AAAACATTGCCTGCCCGCAGTATTGTGTTGCTGCATCCATGTTGCCACAACCCAACGGGTGCCGATCTCACTAATGATCAGTGGGATGCGGTGA          >  SRR4302533.316144/1‑94 (MQ=60)

                                                                                      AAACATTGCCTGCCCGCAGTATTGTGTTGCTGCATCCATGTTGCCACAACCCAACGGGTGCCGATCTCACTAATGATCAGTGGGATGCGGTGAT         >  SRR4302533.4685237/1‑94 (MQ=60)

                                                                                       AACATTGCCTGCCCGCAGTATTGTGTTGCTGCATCCATGTTGCCACAACCCAACGGGTGCCGATCTCACTAATGATCAGTGGGATGCGGTGATT        <  SRR4302533.1968899/94‑1 (MQ=60)

                                                                                       AACATTGCCTGCCCGCAGTATTGTGTTGCTGCATCCATGTTGCCACAACCCAACGGGTGCCGATCTCACTAATGATCAGTGGGATGCGGTGATT        <  SRR4302533.689888/94‑1 (MQ=60)

                                                                                        ACATTGCCTGCCCGCAGTATTGTGTTGCTGCATCCATGTTGCCACAACCCAACGGGTGCCGATCTCACTAATGATCAGTGGGATGCGGTGATTG       >  SRR4302533.1618334/1‑94 (MQ=60)

                                                                                            TGCCTGCCCGCAGTATTGTGTTGCTGCATCCATGTTGCCACAACCCAACGGGTGCCGATCTCACTCATGATCAGTGGGATGCGGTGATTGAAAT   >  SRR4302533.2915496/1‑94 (MQ=60)

                                                                                            TGCCTGCCCGCAGTATTGTGTTGCTGCATCCATGTTGCCACAACCCAACGGGTGCCGATCTCACTAATGATCAGTGGGATGCGGTGATTGAAAT   >  SRR4302533.2915497/1‑94 (MQ=60)

                                                                                             GCCTGCCCGCAGTATTGTGTTGCTGCATCCATGTTGCCACAACCCAACGGGTGCCGATCTCACTAATGATCAGTGGGATGCGGTGATTGAAATT  >  SRR4302533.2951708/1‑94 (MQ=60)

CGGGGCTGGATTCGAAGTGAGTACTTACCCCTGGTATGACGAAGCGACTAACGGCGTGCGCTTTAATGACCTGTTGGCGACGCTGAAAACATTACCTGCCCGCAGTATTGTGTTGCTGCATCCATGTTGCCACAACCCAACGGGTGCCGATCTCACTAATGATCAGTGGGATGCGGTGATTGAAATT  >  NC_000913/4265109‑4265295

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`