Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,291,990 T→C intergenic (+272/‑70) nrfG → / → gltP part of formate‑dependent nitrite reductase complex/glutamate‑aspartate symport protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,291,9900TC100.0% 854.4 / NA 263intergenic (+272/‑70)nrfG/gltPpart of formate‑dependent nitrite reductase complex/glutamate‑aspartate symport protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (117/146);  total (117/146)

Only 100 of 266 total aligned reads displayed.

CTCTGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGATCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCATGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGAACGGCGCTCCATTGAGGAAGTCATTCATATGAAAAATATAAAATTCAG  >  NC_000913/4291900‑4292079
                                                                                          |                                                                                         
ctctGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGATCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCACGc                                                                                         >  1:6045713/1‑93 (MQ=255)
 tctGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGATCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCACGcc                                                                                        >  1:4183613/1‑93 (MQ=255)
 tctGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGATCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCACGcc                                                                                        >  2:802112/1‑93 (MQ=255)
 tctGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGATCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCACGcc                                                                                        >  2:3759721/1‑93 (MQ=255)
    gttgttAAAGGGTTGCGCAACATACCGCGCAAATGATACTGATCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCACGCCCTg                                                                                     <  2:2025468/93‑1 (MQ=255)
    gttgttAAAGGGTTGCGCAACATACCGCGCAAATGATACTGATCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCACGCCCTg                                                                                     <  1:2429005/93‑1 (MQ=255)
     ttgttAAATGGATGCCCAACATACCGCGCAAATGAGTCTGATCATAAGCGTTAAAAAAATCTACAAGCCAACGCAACACAATTCACGCCCTgg                                                                                    <  2:5633158/93‑1 (MQ=255)
     ttgttAAAGGGTTGCGCAACATACCGCGCAAATGATACTGATCATAAGCGTTAAAAAAATCTACAAACCAATGCAACACAATTCACGCCCTgg                                                                                    >  2:644581/1‑93 (MQ=255)
      tgttAAAGGGTTGCGCAACATACCGCGCAAATGATACTGATCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCACGCCCTGGc                                                                                   <  1:3475224/93‑1 (MQ=255)
       gttAAAGGGTTGCGCAACATACCGCGCAAATGATACTGATCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCACGCCCTGGCa                                                                                  >  2:3509458/1‑93 (MQ=255)
        ttAAAGGGTTGCGCAACATACCGCGCAAATGATACTGATCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCACGCCCTGGCAg                                                                                 <  2:5659136/93‑1 (MQ=255)
         tAAAGGGTTGCGCAACATACCGCGCAAATGATACTGATCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCACGCCCTGGCAGt                                                                                >  1:6260159/1‑93 (MQ=255)
          aaaGGGTTGCGCAACATACCGCGCAAATGATACTGATCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCACGCCCTGGCAGTa                                                                               <  1:4141336/93‑1 (MQ=255)
          aaaGGGTTGCGCAACATACCGCGCAAATGATACTGATCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCACGCCCTGGCAGTa                                                                               >  1:2746837/1‑93 (MQ=255)
          aaaGGGTTGCGCAACATACCGCGCAAATGATACTGATCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCACGCCCTGGCAGTa                                                                               <  1:1333730/93‑1 (MQ=255)
             gggTTGCGCAACATACCGCGCAAATGATACTGATCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCACGCCCTGGCAGTATGt                                                                            >  1:4456715/1‑93 (MQ=255)
             gggTTGCGCAACATACCGCGCAAATGATACTGATCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCACGCCCTGGCAGTATGt                                                                            >  1:898074/1‑93 (MQ=255)
              ggTTGCGCAACATACCGCGCAAATGATACTGATCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCACGCCCTGGCAGTATGTc                                                                           <  2:3862858/93‑1 (MQ=255)
                 tGCGCAACATACCGCGCAAATGATACTGATCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCACGCCCTGGCAGTATGTCACg                                                                        <  1:4277131/93‑1 (MQ=255)
                     cAACATACCGCGCAAATGATACTGATCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCACGCCCTGGCAGTATGTCACGTtct                                                                    >  2:3216545/1‑93 (MQ=255)
                     cAACATACCGCGCAAATGATACTGATCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCACGCCCTGGCAGTATGTCACGTtct                                                                    <  1:5857102/93‑1 (MQ=255)
                     cAACATACCGCGCAAATGATACTGATCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCACGCCCTGGCAGTATGTCACGTtct                                                                    <  1:733688/93‑1 (MQ=255)
                      aaCATACCGCGCAAATGATACTGATCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCACGCCCTGGCAGTATGTCACGTtctc                                                                   >  1:5966808/1‑93 (MQ=255)
                      aaCATACCGCGCAAATGATACTGATCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCACGCCCTGGCAGTATGTCACGTtctc                                                                   >  2:4376858/1‑93 (MQ=255)
                       aCATACCGCGCAAATGATACTGATCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCACGCCCTGGCAGTATGTCACGTTCTcg                                                                  <  1:3156549/93‑1 (MQ=255)
                            ccGCGCAAATGATACTGATCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCACGCCCTGGCAGTATGTCACGTTCTCGCGttt                                                             >  1:5916525/1‑93 (MQ=255)
                              gcgcAAATGATACTGATCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCACGCCCTGGCAGTATGTCACGTTCTCGCGTTTCt                                                           >  2:3187094/1‑93 (MQ=255)
                                 cAAATGATACTGATCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCACGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGaa                                                        >  1:1670377/1‑93 (MQ=255)
                                  aaaTGATACTGATCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCACGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAAc                                                       <  2:2103912/93‑1 (MQ=255)
                                  aaaTGATACTGATCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCACGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAAc                                                       >  1:2544659/1‑93 (MQ=255)
                                  aaaTGATACTGATCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCACGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAAc                                                       >  2:4085165/1‑93 (MQ=255)
                                  aaaTGATACTGATCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCACGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAAc                                                       <  1:108586/93‑1 (MQ=255)
                                   aaTGATACTGATCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCACGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACg                                                      >  2:1295963/1‑93 (MQ=255)
                                   aaTGATACTGATCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCACGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACg                                                      >  2:1801209/1‑93 (MQ=255)
                                   aaTGATACTGATCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCACGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACg                                                      >  2:1163159/1‑93 (MQ=255)
                                    aTGATACTGATCATAAGCGTT‑AAAAAATCTACAAACCAACGCAACACAATTCACGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACggg                                                    <  1:5468004/93‑1 (MQ=255)
                                     tGATACTGATCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCACGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACggg                                                    <  2:2861052/93‑1 (MQ=255)
                                      gATACTGATCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCACGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACgggg                                                   <  1:5349523/93‑1 (MQ=255)
                                      gATACTGATCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCACGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACgggg                                                   >  1:435283/1‑93 (MQ=255)
                                       aTACTGATCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCACGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGa                                                  <  2:3437943/93‑1 (MQ=255)
                                       aTACTGATCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCACGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGa                                                  >  1:97068/1‑93 (MQ=255)
                                        tACTGATCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCACGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGaa                                                 >  1:3124399/1‑93 (MQ=255)
                                        tACTGATCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCACGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGaa                                                 >  2:1857711/1‑93 (MQ=255)
                                         aCTGATCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCACGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGAAc                                                <  2:4257023/93‑1 (MQ=255)
                                         aCTGATCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCACGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGAAc                                                <  2:5693543/93‑1 (MQ=255)
                                           tGATCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCACGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGAACgg                                              >  2:836780/1‑93 (MQ=255)
                                              tCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCACGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGAACGgcgc                                           <  2:5873103/93‑1 (MQ=255)
                                              tCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCACGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGAACGgcgc                                           <  1:2105255/93‑1 (MQ=255)
                                              tCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCACGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGAACGgcgc                                           <  1:2347581/93‑1 (MQ=255)
                                               cATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCACGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGAACGGCGCt                                          <  1:4004692/93‑1 (MQ=255)
                                               cATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCACGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGAACGGCGCt                                          >  1:3483387/1‑93 (MQ=255)
                                               cATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCACGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGAACGGCGCt                                          >  2:2819580/1‑93 (MQ=255)
                                                 tAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCACGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGAACGGCGCTcc                                        >  2:4990864/1‑93 (MQ=255)
                                                   aGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCACGCCCTGGCAGTATGTCCCGTTCTCGCGTTTCTGAACGGGGAACGGCGCTCCAt                                      <  2:6232530/93‑1 (MQ=255)
                                                   aGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCACGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGAACGGCGCTCCAt                                      <  2:1444416/93‑1 (MQ=255)
                                                    gCGTTAAAAAAATCTACAAACCAACGCAACACAATTCACGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGAACGGCGCTCCAtt                                     >  1:5083765/1‑93 (MQ=255)
                                                      gTTAAAAAAATCTACAAACCAACGCAACACAATTCACGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGAACGGCGCTCCATTGa                                   <  2:6098706/93‑1 (MQ=255)
                                                          aaaaaaTCTACAAACCAACGCAACACAATTCACGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGAACGGCGCTCCATTGAGGaa                               <  2:1053008/93‑1 (MQ=255)
                                                          aaaaaaTCTACAAACCAACGCAACACAATTCACGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGAACGGCGCTCCATTGAGGaa                               <  2:2753761/93‑1 (MQ=255)
                                                           aaaaaTCTACAAACCAACGCAACACAATTCACGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGAACGGCGCTCCATTGAGGAAg                              >  1:4532641/1‑93 (MQ=255)
                                                           aaaaaTCTACAAACCAACGCAACACAATTCACGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGAACGGCGCTCCATTGAGGAAg                              <  2:3775611/93‑1 (MQ=255)
                                                           aaaaaTCTACAAACCAACGCAACACAATTCACGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGAACGGCGCTCCATTGAGGAAg                              <  2:2432474/93‑1 (MQ=255)
                                                             aaaTCTACAAACCAACGCAACACAATTCACGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGAACGGCGCTCCATTGAGGAAGtc                            <  1:2254491/93‑1 (MQ=255)
                                                                tCTACAAACCAACGCAACACAATTCACGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGAACGGCGCTCCATTGAGGAAGtcatt                         >  2:2613530/1‑93 (MQ=255)
                                                                tCTACAAACCAACGCAACACAATTCACGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGAACGGCGCTCCATTGAGGAAGtcatt                         >  2:1933919/1‑93 (MQ=255)
                                                                tCTACAAACCAACGCAACACAATTCACGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGAACGGCGCTCCATTGAGGAAGtcatt                         >  1:2856124/1‑93 (MQ=255)
                                                                tCTACAAACCAACGCAACACAATGCACGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGAACGGCGCTCCATTGAGGAAGtcatt                         <  1:4815794/93‑1 (MQ=255)
                                                                 cTACAAACCAACGCAACACAATTCACGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGAACGGCGCTCCATTGAGGAAGtcattc                        <  1:6289537/93‑1 (MQ=255)
                                                                 cTACAAACCAACGCAACACAATTCACGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGAACGGCGCTCCATTGAGGAAGtcattc                        <  2:3720301/93‑1 (MQ=255)
                                                                  tACAAACCAACGCAACACAATTCACGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGAACGGCGCTCCATTGAGGAAGtcattca                       <  2:4852310/93‑1 (MQ=255)
                                                                   aCAAACCAACGCAACACAATTCACGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGAACGGCGCTCCATTGAGGAAGtcattcat                      <  2:3943916/93‑1 (MQ=255)
                                                                    cAAACCAACGCAACACAATTCACGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGAACGGCGCTCCATTGAGGAAGTCATTCata                     >  1:1540891/1‑93 (MQ=255)
                                                                     aacccAACGCAACACAATTCACGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGAACGGCGCTCCATTGAGGAAGTCATTCatat                    <  1:3357417/90‑1 (MQ=255)
                                                                     aaaCCAACGCAACACAATTCACGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGAACGGCGCTCCATTGAGGAAGTCATTCatat                    <  2:3035726/93‑1 (MQ=255)
                                                                     aaaCCAACGCAACACAATTCACGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGAACGGCGCTCCATTGAGGAAGTCATTCatat                    <  1:1462304/93‑1 (MQ=255)
                                                                      aaCCAACGCAACACAATTCACGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGAACGGCGCTCCATTGAGGAAGTCATTCATATg                   <  2:5160394/93‑1 (MQ=255)
                                                                      aaCCAACGCAACACAATTCACGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGAACGGCGCTCCATTGAGGAAGTCATTCATATg                   <  2:4795554/93‑1 (MQ=255)
                                                                      aaCCAACGCAACACAATTCACGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGAACGGCGCTCCATTGAGGAAGTCATTCATATg                   <  2:1413957/93‑1 (MQ=255)
                                                                       aCCAACGCAACACAATTCACGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGAACGGCGCTCCATTGAGGAAGTCATTCATATGa                  >  1:1745984/1‑93 (MQ=255)
                                                                         cAACGCAACACAATTCACGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGAACGGCGCTCCATTGAGGAAGTCATTCATATGaaa                >  1:4866853/1‑93 (MQ=255)
                                                                          aaCGCAACACAATTCACGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGAACGGCGCTCCATTGAGGAAGTCATTCATATGaaaa               >  2:4012955/1‑93 (MQ=255)
                                                                          aaCGCAACACAATTCACGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGAACGGCGCTCCATTGAGGAAGTCATTCATATGaaaa               <  2:6000265/93‑1 (MQ=255)
                                                                          aaCGCAACACAATTCACGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGAACGGCGCTCCATTGAGGAAGTCATTCATATGaaaa               <  2:365348/93‑1 (MQ=255)
                                                                          aaCGCAACACAATTCACGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGAACGGCGCTCCATTGAGGAAGTCATTCATATGaaaa               >  1:2680327/1‑93 (MQ=255)
                                                                            cGCAACACAATTCACGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGAACGGCGCTCCATTGAGGAAGTCATTCATATGAAAAat             >  2:5365091/1‑93 (MQ=255)
                                                                             gCAACACAATTCACGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGAACGGCGCTCCATTGAGGAAGTCATTCATATGAAAAata            <  2:470891/93‑1 (MQ=255)
                                                                             gCAACACAATTCACGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGAACGGCGCTCCATTGAGGAAGTCATTCATATGAAAAata            >  2:2543323/1‑93 (MQ=255)
                                                                             gCAACACAATTCACGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGAACGGCGCTCCATTGAGGAAGTCATTCATATGAAAAata            <  2:3327444/93‑1 (MQ=255)
                                                                              cAACACAATTCACGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGAACGGCGCTCCATTGAGGAAGTCATTCATATGAAAAatat           >  2:2120124/1‑93 (MQ=255)
                                                                              cAACACAATTCACGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGAACGGCGCTCCATTGAGGAAGTCATTCATATGAAAAatat           >  1:5328097/1‑93 (MQ=255)
                                                                               aaCACAATTCACGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGAACGGCGCTCCATTGAGGAAGTCATTCATATGAAAAAtata          <  2:3470566/93‑1 (MQ=255)
                                                                               aaCACAATTCACGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGAACGGCGCTCCATTGAGGAAGTCATTCATATGAAAAAtata          <  1:1000793/93‑1 (MQ=255)
                                                                                acacAATTCACGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGAACGGCGCTCCATTGAGGAAGTCATTCATATGAAAAATATaa         >  1:6144339/1‑93 (MQ=255)
                                                                                acacAATTCACGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGAACGGCGCTCCATTGAGGAAGTCATTCATATGAAAAATATaa         <  1:361403/93‑1 (MQ=255)
                                                                                acacAATTCACGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGAACGGCGCTCCATTGAGGAAGTCATTCATATGAAAAATATaa         >  1:3634981/1‑93 (MQ=255)
                                                                                   caATTCACGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGAACGGCGCTCCATTGAGGAAGTCATTCATATGAAAAATATAAAAt      <  1:3836330/93‑1 (MQ=255)
                                                                                    aaTTCACGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGAACGGCGCTCCATTGAGGAAGTCATTCATATGAAAAATATAAAAtt     <  1:5796730/93‑1 (MQ=255)
                                                                                    aaTTCACGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGAACGGCGCTCCATTGAGGAAGTCATTCATATGAAAAATATAAAAtt     <  2:3693252/93‑1 (MQ=255)
                                                                                     aTTCACGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGAACGGCGCTCCATTGAGGAAGTCATTCATATGAAAAATATAAAATTc    <  1:185729/93‑1 (MQ=255)
                                                                                      ttCACGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGAACGGCGCTCCATTGAGGAAGTCATTCATATGAAAAATATAAAATTCa   <  1:1397469/93‑1 (MQ=255)
                                                                                          |                                                                                         
CTCTGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGATCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCATGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGAACGGCGCTCCATTGAGGAAGTCATTCATATGAAAAATATAAAATTCAG  >  NC_000913/4291900‑4292079

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AAGCTCTGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGATCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCATGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGAACGGCGCTCCATTGAGGAAGTCATTCATATGAAAAATATAAAATTCAGCCTG  >  NC_000913/4291897‑4292083
                                                                                             |                                                                                             
AAGCTCTGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGATCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCAC                                                                                               >  SRR4302533.3114081/1‑94 (MQ=60)
AAGCTCTGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGATCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCAC                                                                                               <  SRR4302533.5888848/94‑1 (MQ=60)
AAGCTCTGTTGTAAAAGGGTTGCGCAACATACCGCGCAAATGATACTGATCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCAC                                                                                               <  SRR4302533.5108530/94‑1 (MQ=60)
 AGCTCTGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGATCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCACG                                                                                              >  SRR4302533.5504356/1‑94 (MQ=60)
 AGCTCTGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGATCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCACG                                                                                              >  SRR4302533.4392225/1‑94 (MQ=60)
 AGCTCTGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGATCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCACG                                                                                              <  SRR4302533.901977/94‑1 (MQ=60)
  GCTCTGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGATCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCACGC                                                                                             >  SRR4302533.6082341/1‑94 (MQ=60)
   CTCTGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGATCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCACGCC                                                                                            >  SRR4302533.2446503/1‑94 (MQ=60)
   CTCTGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGATCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCACGCC                                                                                            >  SRR4302533.4208947/1‑94 (MQ=60)
     CTGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGATCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCACGCCCT                                                                                          >  SRR4302533.1628291/1‑94 (MQ=60)
      TGTTGTTAAGGGGTTGCGCAACATACCGCGCAAATGATACTGATCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCACGCCCTG                                                                                         <  SRR4302533.599591/94‑1 (MQ=60)
      TGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGATCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCACGCCCTG                                                                                         <  SRR4302533.4316103/94‑1 (MQ=60)
      TGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGATCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCACGCCCTG                                                                                         >  SRR4302533.3419896/1‑94 (MQ=60)
       GTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGATCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCACGCCCTGG                                                                                        <  SRR4302533.724882/94‑1 (MQ=60)
       GTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGATCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCACGCCCTGG                                                                                        <  SRR4302533.1471092/94‑1 (MQ=60)
       GTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGATCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCACGCCCTGG                                                                                        <  SRR4302533.2444021/94‑1 (MQ=60)
         TGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGATCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCACGCCCTGGCA                                                                                      <  SRR4302533.3496667/94‑1 (MQ=60)
           TTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGATCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCACGCCCTGGCAGT                                                                                    >  SRR4302533.6297586/1‑94 (MQ=60)
            TAAAGGGTTGCGCAACATACCGCGCAAATGATACTGATCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCACGCCCTGGCAGTA                                                                                   >  SRR4302533.2763678/1‑94 (MQ=60)
            TAAAGGGTTGCGCAACATACCGCGCAAATGATACTGATCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCACGCCCTGGCAGTA                                                                                   >  SRR4302533.3648269/1‑94 (MQ=60)
            TAAAGGGTTGCGCAACATACCGCGCAAATGATACTGATCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCACGCCCTGGCAGTA                                                                                   >  SRR4302533.931496/1‑94 (MQ=60)
             AAAGGGTTGCGCAACATACCGCGCAAATGATACTGATCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCACGCCCTGGCAGTAT                                                                                  <  SRR4302533.3248425/94‑1 (MQ=60)
             AAAGGGTTGCGCAACATACCGCGCAAATGATACTGATCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCACGCCCTGGCAGTAT                                                                                  <  SRR4302533.2650855/94‑1 (MQ=60)
             AAAGGGTTGCGCAACATACCGCGCAAATGATACTGATCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCACGCCCTGGCAGTAT                                                                                  <  SRR4302533.1341736/94‑1 (MQ=60)
             AAAGGGTTGCGCAACATACCGCGCAAATGATACTGATCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCACGCCCTGGCAGTAT                                                                                  <  SRR4302533.4166450/94‑1 (MQ=60)
             AAAGGGTTGCGCAACATACCGCGCAAATGATACTGATCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCACGCCCTGGCAGTAT                                                                                  <  SRR4302533.1535052/94‑1 (MQ=60)
               AGGGTTGCGCAACATACCGCGCAAATGATACTGATCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCACGCCCTGGCAGTATGT                                                                                >  SRR4302533.4483664/1‑94 (MQ=60)
               AGGGTTGCGCAACATACCGCGCAAATGATACTGATCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCACGCCCTGGCAGTATGT                                                                                >  SRR4302533.903344/1‑94 (MQ=60)
                 GGTTGCGCAACATACCGCGCAAATGATACTGATCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCACGCCCTGGCAGTATGTCA                                                                              >  SRR4302533.4248812/1‑94 (MQ=60)
                  GTTGCGCAACATACCGCGCAAATGATACTGATCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCACGCCCTGGCAGTATGTCAC                                                                             >  SRR4302533.2720798/1‑94 (MQ=60)
                    TGCGCAACATACCGCGCAAATGATACTGATCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCACGCCCTGGCAGTATGTCACGT                                                                           <  SRR4302533.4302853/94‑1 (MQ=60)
                    TGCGCAACATACCGCGCAAATGATACTGATCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCACGCCCTGGCAGTATGTCACGT                                                                           <  SRR4302533.5378060/94‑1 (MQ=60)
                     GCGCAACATACCGCGCAAATGATACTGATCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCACGCCCTGGCAGTATGTCACGTT                                                                          <  SRR4302533.619092/94‑1 (MQ=60)
                      CGCAACATACCGCGCAAATGATACTGATCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCACGCCCTGGCAGTATGTCACGTTC                                                                         >  SRR4302533.4145510/1‑94 (MQ=60)
                        CAACATACCGCGCAAATGATACTGATCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCACGCCCTGGCAGTATGTCACGTTCTC                                                                       <  SRR4302533.738004/94‑1 (MQ=60)
                        CAACATACCGCGCAAATGATACTGATCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCACGCCCTGGCAGTATGTCACGTTCTC                                                                       >  SRR4302533.6002927/1‑94 (MQ=60)
                        CAACATACCGCGCAAATGATACTGATCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCACGCCCTGGCAGTATGTCACGTTCTC                                                                       <  SRR4302533.5892509/94‑1 (MQ=60)
                          ACATACCGCGCAAATGATACTGATCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCACGCCCTGGCAGTATGTCACGTTCTCGC                                                                     <  SRR4302533.3175959/94‑1 (MQ=60)
                              ACCGCGCAAATGATACTGATCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCACGCCCTGGCAGTATGTCACGTTCTCGCGTTT                                                                 >  SRR4302533.5952282/1‑94 (MQ=60)
                              ACCGCGCAAATGATACTGATCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCACGCCCTGGCAGTATGTCACGTTCTCGCGTTT                                                                 <  SRR4302533.3562045/94‑1 (MQ=60)
                              ACCGCGCAAATGATACTGATCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCACGCCCTGGCAGTATGTCACGTTCTCGCGTTT                                                                 >  SRR4302533.2147379/1‑94 (MQ=60)
                               CCGCGCAAATGATACTGATCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCACGCCCTGGCAGTATGTCACGTTCTCGCGTTTC                                                                <  SRR4302533.1847542/94‑1 (MQ=60)
                                CGCGCAAATGATACTGATCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCACGCCCTGGCAGTATGTCACGTTCTCGCGTTTCT                                                               >  SRR4302533.585635/1‑94 (MQ=60)
                                  CGCAAATGATACTGATCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCACGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGA                                                             >  SRR4302533.3735084/1‑94 (MQ=60)
                                   GCAAATGATACTGATCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCACGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAA                                                            >  SRR4302533.1680472/1‑94 (MQ=60)
                                    CAAATGATACTGATCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCACGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAAC                                                           >  SRR4302533.2560318/1‑94 (MQ=60)
                                     AAATGATACTGATCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCACGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACG                                                          <  SRR4302533.109271/94‑1 (MQ=60)
                                     AAATGATACTGATCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCACGCCCTGGCAGCATGTCACGTTCTCGCGCTTCTGAACG                                                          >  SRR4302533.108525/1‑94 (MQ=60)
                                      AATGATACTGATCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCACGCCCTGGCAGTATGTCACGTTCTCGCGTCTCTGAACGG                                                         >  SRR4302533.6177601/1‑94 (MQ=60)
                                       ATGATACTGATCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCACGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGG                                                        <  SRR4302533.1699927/94‑1 (MQ=60)
                                       ATGATACTGATCATAAGCGTT‑AAAAAATCTACAAACCAACGCAACACAATTCACGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGGG                                                       <  SRR4302533.5500959/94‑1 (MQ=60)
                                        TGATACTGATCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCACGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGGG                                                       >  SRR4302533.437878/1‑94 (MQ=60)
                                         GATACTGATCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCACGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGA                                                      <  SRR4302533.4709459/94‑1 (MQ=60)
                                         GATACTGATCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCACGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGA                                                      >  SRR4302533.97673/1‑94 (MQ=60)
                                         GATACTGATCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCACGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGA                                                      <  SRR4302533.5381621/94‑1 (MQ=60)
                                          ATACTGATCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCACGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGAA                                                     >  SRR4302533.3143604/1‑94 (MQ=60)
                                          ATACTGATCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCACGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGAA                                                     <  SRR4302533.4341688/94‑1 (MQ=60)
                                          ATACTGATCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCACGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGAA                                                     <  SRR4302533.216683/94‑1 (MQ=60)
                                           TACTGATCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCACGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGAAC                                                    <  SRR4302533.1747231/94‑1 (MQ=60)
                                            ACTGATCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCACGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGAACG                                                   <  SRR4302533.2798403/94‑1 (MQ=60)
                                             CTGATCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCACGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGAACGG                                                  >  SRR4302533.4221913/1‑94 (MQ=60)
                                              TGATCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCACGCCCTGGCAGTATGTCAAGTTCTCGCGTTTCTGAACGGGGAACGGC                                                 <  SRR4302533.3655695/94‑1 (MQ=60)
                                                ATCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCACGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGAACGGCGC                                               >  SRR4302533.3114181/1‑94 (MQ=60)
                                                 TCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCACGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGAACGGCGCT                                              >  SRR4302533.3504874/1‑94 (MQ=60)
                                                 TCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCACGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGAACGGCGCT                                              <  SRR4302533.2118238/94‑1 (MQ=60)
                                                 TCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCACGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGAACGGCGCT                                              >  SRR4302533.326418/1‑94 (MQ=60)
                                                 TCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCACGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGAACGGCGCT                                              <  SRR4302533.2362105/94‑1 (MQ=60)
                                                  CATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCACGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGAACGGCGCTC                                             <  SRR4302533.4029070/94‑1 (MQ=60)
                                                    TAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCACGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGAACGGCGCTCCA                                           <  SRR4302533.810669/94‑1 (MQ=60)
                                                    TAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCACGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGAACGGCGCTCCA                                           >  SRR4302533.1298584/1‑94 (MQ=60)
                                                    TAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCACGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGAACGGCGCTCCA                                           <  SRR4302533.3052951/94‑1 (MQ=60)
                                                     AAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCACGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGAACGGCGCTCCAT                                          >  SRR4302533.812838/1‑94 (MQ=60)
                                                      AGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCACGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGAACGGCGCTCCATT                                         >  SRR4302533.5114490/1‑94 (MQ=60)
                                                         GTTAAAAAAATCTACAAACCAACGCAACACAATTCACGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGAACGGCGCTCCATTGAG                                      >  SRR4302533.3148063/1‑94 (MQ=60)
                                                           TAAAAAAATCTACAAACCAACGCAACACAATTCACGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGAACGGCGCTCCATTGAGGA                                    <  SRR4302533.1291555/94‑1 (MQ=60)
                                                            AAAAAAATCTACAAACCAACGCAACACAATTCACGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGAACGGCGCTCCATTGAGGAA                                   <  SRR4302533.1976177/94‑1 (MQ=60)
                                                            AAAAAAATCTACAAACCAACGCAACACAATTCACGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGAACGGCGCTCCATTGAGGAA                                   <  SRR4302533.4329973/94‑1 (MQ=60)
                                                             AAAAAATCTACAAACCAACGCAACACAATTCACGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGAACGGCGCTCCATTGAGGAAG                                  >  SRR4302533.4560085/1‑94 (MQ=60)
                                                                AAATCTACAAACCAACGCAACACAATTCACGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGAACGGCGCTCCATTGAGGAAGTCA                               >  SRR4302533.2437931/1‑94 (MQ=60)
                                                                AAATCTACAAACCAACGCAACACAATTCACGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGAACGGCGCTCCATTGAGGAAGTCA                               <  SRR4302533.2268432/94‑1 (MQ=60)
                                                                  ATCTACAAACCAACGCAACACAATTCACGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGAACGGCGCTCCATTGAGGAAGTCATT                             >  SRR4302533.2873699/1‑94 (MQ=60)
                                                                  ATCTACAAACCAACGCAACACAATTCACGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGAACGGCGCTCCATTGAGGAAGTCATT                             >  SRR4302533.2320674/1‑94 (MQ=60)
                                                                   TCTACAAACCAACGCAACACAATGCACGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGAACGGCGCTCCATTGAGGAAGTCATTC                            <  SRR4302533.4844980/94‑1 (MQ=60)
                                                                   ACTACAACCCAACGCAACACAATTCACGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGTGAACGGCGCTCCATTGAGGAAGTCATTC                            <  SRR4302533.977025/94‑1 (MQ=60)
                                                                    CTACAAACCAACGCAACACAATTCACGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGAACGGCGCTCCATTGAGGAAGTCATTCA                           >  SRR4302533.1530973/1‑94 (MQ=60)
                                                                    CTACAAACCAACGCAACACAATTCACGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGAACGGCGCTCCATTGAGGAAGTCATTCA                           <  SRR4302533.6327049/94‑1 (MQ=60)
                                                                    CTACAAACCAACGCAACACAATTCACGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGAACGGCGCTCCATTGAGGAAGTCATTCA                           >  SRR4302533.4028897/1‑94 (MQ=60)
                                                                    CTACAAACCAACGCAACACAATTCACGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGAACGGCGCTCCATTGAGGAAGTCATTCA                           <  SRR4302533.2568530/94‑1 (MQ=60)
                                                                     TACAAACCAACGCAACACAATTCACGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGAACGGCGCTCCATTGAGGAAGTCATTCAT                          >  SRR4302533.5749252/1‑94 (MQ=60)
                                                                      ACAAACCAACGCAACACAATTCACGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGAACGGCGCTCCATTGAGGAAGTCATTCATA                         >  SRR4302533.1550172/1‑94 (MQ=60)
                                                                       CAAACCAACGCAACACAATTCACGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGAACGGCGCTCCATTGAGGAAGTCATTCATAT                        <  SRR4302533.4719909/94‑1 (MQ=60)
                                                                        AACCCAACGCAACACAATTCACGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGAACGGCGCTCCATTGAGGAAGTCATTCATATG                       <  SRR4302533.3378165/94‑1 (MQ=60)
                                                                        AAACCAACGCAACACAATTCACGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGAACGGCGCTCCATTGAGGAAGTCATTCATATG                       <  SRR4302533.5045879/94‑1 (MQ=60)
                                                                        AAACCAACGCAACACAATTCACGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGAACGGCGCTCCATTGAGGAAGTCATTCATATG                       <  SRR4302533.1471121/94‑1 (MQ=60)
                                                                        AAACCAACGCAACACAATTCACGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGAACGGCGCTCCATTGAGGAAGTCATTCATATG                       >  SRR4302533.5945080/1‑94 (MQ=60)
                                                                        AAACCAACGCAACACAATTCACGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGAACGGCGCTCCATTGAGGAAGTCATTCATATG                       <  SRR4302533.5045878/94‑1 (MQ=60)
                                                                         AACCAACGCAACACAATTCACGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGAACGGCGCTCCATTGAGGAAGTCATTCATATGA                      <  SRR4302533.2287232/94‑1 (MQ=60)
                                                                         AACCAACGCAACACAATTCACGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGAACGGCGCTCCATTGAGGAAGTCATTCATATGA                      >  SRR4302533.1756604/1‑94 (MQ=60)
                                                                          ACCAACGCAACACAATTCACGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGAACGGCGCTCCATTGAGGAAGTCATTCATATGAA                     <  SRR4302533.5164148/94‑1 (MQ=60)
                                                                           CCAACGCAACACAATTCACGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGAACGGCGCTCCATTGAGGAAGTCATTCATATGAAA                    >  SRR4302533.4896345/1‑94 (MQ=60)
                                                                           CCAACGCAACACAATTCACGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGAACGGCGCTCCATTGAGGAAGTCATTCATATGAAA                    >  SRR4302533.1342523/1‑94 (MQ=60)
                                                                            CAACGCAACACAATTCACGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAGCGGGGAACGGCGCTCCATTGAGGAAGTCATTCATATGAAAA                   <  SRR4302533.3380767/94‑1 (MQ=60)
                                                                            CAACGCAACACAATTCACGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGAACGGCGCTCCATTGAGGAAGTCATTCATATGAAAA                   >  SRR4302533.2696752/1‑94 (MQ=60)
                                                                            CAACGCAACACAATTCACGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGAACGGCGCTCCATTGAGGAAGTCATTCATATGAAAA                   <  SRR4302533.5443126/94‑1 (MQ=60)
                                                                             AACGCAACACAATTCACGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGAACGGCGCTCCATTGAGGAAGTCATTCATATGAAAAA                  >  SRR4302533.6097611/1‑94 (MQ=60)
                                                                               CGCAACACAATTCACGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGAACGGCGCTCCATTGAGGAAGTCATTCATATGAAAAATA                <  SRR4302533.4500184/94‑1 (MQ=60)
                                                                               CGCAACACAATTCACGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGAACGGCGCTCCATTGAGGAAGTCATTCATATGAAAAATA                <  SRR4302533.5480647/94‑1 (MQ=60)
                                                                                GCAACACAATTCACGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGAACGGCGCTCCATTGAGGAAGTCATTCATATGAAAAATAT               <  SRR4302533.982979/94‑1 (MQ=60)
                                                                                GCAACACAATTCACGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGAACGGCGCTCCATTGAGGAAGTCATTCATATGAAAAATAT               <  SRR4302533.6284763/94‑1 (MQ=60)
                                                                                GCAACACAATTCACGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGAACGGCGCTCCATTGAGGAAGTCATTCATATGAAAAATAT               <  SRR4302533.4190252/94‑1 (MQ=60)
                                                                                GCAACACAATTCACGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGAACGGCGCTCCATTGAGGAAGTCATTCATATGAAAAATAT               <  SRR4302533.5507147/94‑1 (MQ=60)
                                                                                GCAACACAATTCACGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGAACGGCGCTCCATTGAGGAAGTCATTCATATGAAAAATAT               >  SRR4302533.5360026/1‑94 (MQ=60)
                                                                                 CAACACAATTCACGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGAACGGCGCTCCATTGAGGAAGTCATTCATATGAAAAATATA              >  SRR4302533.590872/1‑94 (MQ=60)
                                                                                  AACACAATTCACGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGAACGGCGCTCCATTGAGGAAGTCATTCATATGAAAAATATAA             <  SRR4302533.1804338/94‑1 (MQ=60)
                                                                                  AACACAATTCACGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGAACGGCGCTCCATTGAGGAAGTCATTCATATGAAAAATATAA             <  SRR4302533.1006636/94‑1 (MQ=60)
                                                                                  AACACAATTCACGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGAACGGCGCTCCATTGAGGAAGTCATTCATATGAAAAATATAA             >  SRR4302533.3657335/1‑94 (MQ=60)
                                                                                  AACACAATTCACGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGAACGGCGCTCCATTGAGGAAGTCATTCATATGAAAAATATAA             >  SRR4302533.6017902/1‑94 (MQ=60)
                                                                                  AACACAATTCACGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGAACGGCGCTCCATTGAGGAAGTCATTCATATGAAAAATATAA             >  SRR4302533.6181335/1‑94 (MQ=60)
                                                                                   ACACAATTCACGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGAACGGCGCTCCATTGAGGAAGTCATTCATATGAAAAATATAAA            <  SRR4302533.363599/94‑1 (MQ=60)
                                                                                   ACACAATTCACGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGAACGGCGCTCCATTGAGGAAGTCATTCATATGAAAAATATAAA            <  SRR4302533.6303926/94‑1 (MQ=60)
                                                                                   ACACAATTCACGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGAACGGCGCTCCATTGAGGAAGTCATTCATATGAAAAATATAAA            <  SRR4302533.1463853/94‑1 (MQ=60)
                                                                                    CACAATTCACGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGAACGGCGCTCCATTGAGGAAGTCATTCATATGAAAAATATAAAA           <  SRR4302533.4886422/94‑1 (MQ=60)
                                                                                    CACAATTCACGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGAACGGCGCTCCATTGAGGAAGTCATTCATATGAAAAATATAAAA           <  SRR4302533.4466970/94‑1 (MQ=60)
                                                                                      CAATTCACGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGAACGGCGCTCCATTGAGGAAGTCATTCATATGAAAAATATAAAATT         <  SRR4302533.3859771/94‑1 (MQ=60)
                                                                                      CAATTCACGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGAACGGCGCTCCATTGAGGAAGTCATTCATATGAAAAATATAAAATT         >  SRR4302533.522948/1‑94 (MQ=60)
                                                                                      CAATTCACGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGAACGGCGCTCCATTGAGGAAGTCATTCATATGAAAAATATAAAATT         >  SRR4302533.3502053/1‑94 (MQ=60)
                                                                                      CAATTCACGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGAACGGCGCTCCATTGAGGAAGTCATTCATATGAAAAATATAAAATT         <  SRR4302533.68115/94‑1 (MQ=60)
                                                                                       AATTCACGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGAACGGCGCTCCATTGAGGAAGTCATTCATATGAAAAATATAAAATTC        <  SRR4302533.5831752/94‑1 (MQ=60)
                                                                                       AATTCACGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGAACGGCGCTCCATTGAGGAAGTCATTCATATGAAAAATATAAAATTC        >  SRR4302533.3629536/1‑94 (MQ=60)
                                                                                        ATTCACGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGAACGGCGCTCCATTGAGGAAGTCATTCATATGAAAAATATAAAATTCA       <  SRR4302533.186886/94‑1 (MQ=60)
                                                                                        ATTCACGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGAACGGCGCTCCATTGAGGAAGTCATTCATATGAAAAATATAAAATTCA       <  SRR4302533.4092169/94‑1 (MQ=60)
                                                                                        ATTCACGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGAACGGCGCTCCATTGAGGAAGTCATTCATATGAAAAATATAAAATTCA       <  SRR4302533.5956732/94‑1 (MQ=60)
                                                                                         TTCACGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGAACGGCGCTCCATTGAGGAAGTCATTCGTATGAAAAATATAAAATTCAG      <  SRR4302533.2454625/94‑1 (MQ=60)
                                                                                         TTCACGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGAACGGCGCTCCATTGAGGAAGTCATTCATATGAAAAATATAAAATTCAG      <  SRR4302533.1405891/94‑1 (MQ=60)
                                                                                         TTCACGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGAACGGCGCTCCATTGAGGAAGTCATTCATATGAAAAATATAAAATTCAG      <  SRR4302533.28019/94‑1 (MQ=60)
                                                                                          TCACGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGAACGGCGCTCCATTGAGGAAGTCATTCATATGAAAAATATAAAATTCAGC     >  SRR4302533.6296358/1‑94 (MQ=60)
                                                                                          TCACGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGAACGGCGCTCCATTGAGGAAGTCATTCATATGAAAAATATAAAATTCAGC     <  SRR4302533.449204/94‑1 (MQ=60)
                                                                                          TCACGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGAACGGCGCTCCATTGAGGAAGTCATTCATATGAAAAATATAAAATTCAGC     <  SRR4302533.3964100/94‑1 (MQ=60)
                                                                                           CACGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGAACGGCGCTCCATTGAGGAAGTCATTCATATGAATAATATAAAATTCAGCC    <  SRR4302533.544140/94‑1 (MQ=60)
                                                                                            ACGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGAACGGCGCTCCATTGAGGAAGTCATTCATATGAAAAATATAAAATTCAGCCT   <  SRR4302533.2703473/94‑1 (MQ=60)
                                                                                            ACGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGAACGGCGCTCCATTGAGGAAGTCATTCATATGAAAAATATAAAATTCAGCCT   <  SRR4302533.1242254/94‑1 (MQ=60)
                                                                                             CGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGAACGGCGCTCCATTGAGGAAGTCATTCATATGAAAAATATAAAATTCAGCCTG  <  SRR4302533.4377355/94‑1 (MQ=60)
                                                                                             |                                                                                             
AAGCTCTGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGATCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCATGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGAACGGCGCTCCATTGAGGAAGTCATTCATATGAAAAATATAAAATTCAGCCTG  >  NC_000913/4291897‑4292083

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑