Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1624	250.6	12620048	98.0%	12367647	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,427,556	A→G	T39A (ACG→GCG)	cycA →	transport of D‑alanine, D‑serine, and glycine

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,427,556	0	A	G	99.6%	770.3 / NA	241	T39A (ACG→GCG)	cycA	transport of D‑alanine, D‑serine, and glycine
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (100/140); minor base C (0/1); total (100/141)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 248 total aligned reads displayed.

GCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTT  >  NC_000913/4427466‑4427645

    atgatCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTGCGGGgt                                                                                     >  2:4567746/1‑93 (MQ=255)

     tgatCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTGCGGGgtt                                                                                    >  1:4223008/1‑93 (MQ=255)

     tgatCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTGCGGGgtt                                                                                    >  1:2578826/1‑93 (MQ=255)

      gatCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTGCGGGgttg                                                                                   >  1:4954135/1‑93 (MQ=255)

       atCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTGCGGGgttgt                                                                                  >  2:3611554/1‑93 (MQ=255)

       atCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTGCGGGgttgt                                                                                  >  1:810551/1‑93 (MQ=255)

        tCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTGCGGGgttgtt                                                                                 <  1:4530259/93‑1 (MQ=255)

          aGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTGCGGGGTTGTTTa                                                                               <  1:3871229/93‑1 (MQ=255)

          aGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTGCGGGGTTGTTTa                                                                               <  2:3243570/93‑1 (MQ=255)

           ggCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTGCGGGGTTGTTTAt                                                                              <  1:475996/93‑1 (MQ=255)

             cTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTTGTGCGGGGTTGTTTATgg                                                                            >  2:3756734/1‑93 (MQ=255)

                 ggCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTGCGGGGTTGTTTATGGGGTc                                                                        <  1:4311869/93‑1 (MQ=255)

                   cTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTGCGGGGTTGTTTATGGGGTCTg                                                                      <  1:3706585/93‑1 (MQ=255)

                    tGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTGCGGGGTTGTTTATGGGGTCTgg                                                                     <  1:113364/93‑1 (MQ=255)

                     gAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTGCGGGGTTGTTTATGGGGTCTGGc                                                                    >  2:2259706/1‑93 (MQ=255)

                     gAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTGCGGGGTTGTTTATGGGGTCTGGc                                                                    >  2:6303391/1‑93 (MQ=255)

                      aaCAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTGCGGGGTTGTTTATGGGGTCTGGCa                                                                   >  2:378774/1‑93 (MQ=255)

                       aCAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTGCGGGGTTGTTTATGGGGTCTGGCaa                                                                  >  2:5498278/1‑93 (MQ=255)

                       aCAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTGCGGGGTTGTTTATGGGGTCTGGCaa                                                                  >  2:5605229/1‑93 (MQ=255)

                            cGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTGCGGGGTTGTTTATGGGGTCTGGCAAAACGa                                                             <  1:5114001/93‑1 (MQ=255)

                            cGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTGCGGGGTTGTTTATGGGGTCTGGCAAAACGa                                                             <  2:1386852/93‑1 (MQ=255)

                            cGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTGCGGGGTTGTTTATGGGGTCTGGCAAAACGa                                                             <  1:4036063/93‑1 (MQ=255)

                              cTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTGCGGGGTTGTTTATGGGGTCTGGCAAAACGAtt                                                           <  1:693281/93‑1 (MQ=255)

                              cTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTGCGGGGGTGTTTATGGGGTCTGGCAAAACGAtt                                                           >  2:127020/1‑93 (MQ=255)

                               tACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTGCGGGGTTGTTTATGGGGTCTGGCAAAACGATTa                                                          >  2:262663/1‑93 (MQ=255)

                                aCGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTGCGGGGTTGTTTATGGGGTCTGGGAAAACGATTAg                                                         >  2:2984659/1‑93 (MQ=255)

                                 cGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTGCGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGc                                                        <  2:2044097/93‑1 (MQ=255)

                                  ggCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTGCGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGcc                                                       >  2:4692878/1‑93 (MQ=255)

                                   gcgcAATCTCACAAACCGACATATTCAGCTTAGTGCCATTGGCTGTGCCATTGGTGCGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCt                                                      <  1:820496/93‑1 (MQ=255)

                                     acAATCACACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTGCGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTg                                                    <  2:5065002/92‑1 (MQ=255)

                                       aaTCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTGCGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGcc                                                  >  1:238980/1‑93 (MQ=255)

                                       aaTCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTGCGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGcc                                                  <  2:5862545/93‑1 (MQ=255)

                                       aaTCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTGCGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGcc                                                  <  1:2356611/93‑1 (MQ=255)

                                        aTCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTGCGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCg                                                 >  1:2085099/1‑93 (MQ=255)

                                         tctcACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTGCGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCgg                                                <  2:4638741/93‑1 (MQ=255)

                                         tctcACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTGCGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCgg                                                <  1:1302490/93‑1 (MQ=255)

                                         tctcACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTGCGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCgg                                                <  1:2842736/93‑1 (MQ=255)

                                         tctcACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTGCGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCgg                                                >  1:3342614/1‑93 (MQ=255)

                                           tcACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTGCGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGc                                              <  1:1090578/93‑1 (MQ=255)

                                           tcACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTGCGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGc                                              >  2:5594035/1‑93 (MQ=255)

                                            cacaAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTGCGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGcc                                             <  1:5288282/93‑1 (MQ=255)

                                             acaAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTGCGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCg                                            <  1:4079103/93‑1 (MQ=255)

                                              caAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTGCGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGt                                           <  2:4810099/93‑1 (MQ=255)

                                               aaaCCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTGCGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTc                                          <  2:2877101/93‑1 (MQ=255)

                                               aaaCCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTGCGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTc                                          <  2:5660975/93‑1 (MQ=255)

                                                aaCCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTGCGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCg                                         <  2:3120301/93‑1 (MQ=255)

                                                aaCCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTGCGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCg                                         <  2:1908960/93‑1 (MQ=255)

                                                 agcGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTGCGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGa                                        >  2:3972973/3‑93 (MQ=255)

                                                 aCCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTGCGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGa                                        <  1:5667520/93‑1 (MQ=255)

                                                  ccGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTGCGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGAt                                       <  2:1478554/93‑1 (MQ=255)

                                                   cGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTGCGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATc                                      >  2:5300594/1‑93 (MQ=255)

                                                   cGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTGCGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATc                                      >  2:1070196/1‑93 (MQ=255)

                                                    gACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTGCGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCa                                     >  1:4577148/1‑93 (MQ=255)

                                                    gACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTGCGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCa                                     <  1:3247746/93‑1 (MQ=255)

                                                    gACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTGCGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCa                                     >  1:1166046/1‑93 (MQ=255)

                                                    gACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTGCGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCa                                     <  1:1720922/93‑1 (MQ=255)

                                                     aCATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTGCGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCAt                                    >  1:2292903/1‑93 (MQ=255)

                                                      cATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTGCGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCAtt                                   >  2:4311122/1‑93 (MQ=255)

                                                      cATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTGCGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCAtt                                   >  2:4529065/1‑93 (MQ=255)

                                                       atatTCAGCTTATTGCCATTGGCGGTGCCATTGGTGCGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCAttt                                  >  1:2191630/1‑93 (MQ=255)

                                                        tatTCAGCTTATTGCCATTGGCGGTGCCATTGGTGCGGGGTTGTTTTTGGGTCTTGGCAAAACAATT‑GCCCTGCCGGGCCGTCGGTCATTTTc                                >  1:173867/1‑93 (MQ=255)

                                                        tatTCAGCTTATTGCCATTGGCGGTGCCATTGGTGCGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCAtttt                                 <  1:1279449/93‑1 (MQ=255)

                                                         atTCAGCTTATTGCCATTGGCGGTGCCATTGGTGCGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTc                                <  1:1875349/93‑1 (MQ=255)

                                                         atTCAGCTTATTGCCATTGGCGGTGCCATTGGTGCGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTc                                <  1:5130952/93‑1 (MQ=255)

                                                             aGCTTATTGCCATTGGCGGTGCCATTGGTGCGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGttt                            >  2:3284098/1‑93 (MQ=255)

                                                             aGCTTATTGCCATTGGCGGTGCCATTGGTGCGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGttt                            <  2:2182915/93‑1 (MQ=255)

                                                               cTTATTGCCATTGGCGGTGCCATTGGTGCGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTtat                          <  1:5324582/93‑1 (MQ=255)

                                                                ttATTGCCATTGGCGGTGCCATTGGTGCGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTtata                         >  2:2488139/1‑93 (MQ=255)

                                                                ttATTGCCATTGGCGGTGCCATTGGTGCGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTtata                         <  2:736127/93‑1 (MQ=255)

                                                                ttATTGCCATTGGCGGTGCCATTGGTGCGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTtata                         >  1:2154425/1‑93 (MQ=255)

                                                                 tATTGCCATTGGCGGTGCCATTGGTGCGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTgtat                        <  1:264228/93‑1 (MQ=255)

                                                                 tATTGCCATTGGCGGTGCCATTGGTGCGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTatat                        >  1:899942/1‑93 (MQ=255)

                                                                  aTTGCCATTGGCGGTGCCATTGGTGCGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATg                       <  2:4178686/93‑1 (MQ=255)

                                                                   ttGCCATTGGCGGTGCCATTGGTGCGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGa                      >  1:4697077/1‑93 (MQ=255)

                                                                   ttGCCATTGGCGGTGCCATTGGTGCGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGa                      >  1:2486856/1‑93 (MQ=255)

                                                                     tccATTGGCGGTGCCATTGGTGCGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATc                    <  1:1637195/92‑1 (MQ=255)

                                                                       cATTGGCGGTGCCATTGGTGCGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCAt                  >  2:5202125/1‑93 (MQ=255)

                                                                       cATTGGCGGTGCCATTGGTGCGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCAt                  <  1:4803439/93‑1 (MQ=255)

                                                                        aTTGGCGGTGCCATTGGTGCGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCAtt                 <  2:4892649/93‑1 (MQ=255)

                                                                        aTTGCCGGTGCCATTGGTGCGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCATCGATCATTTTCGTTTATATGATCAtt                 <  2:5422280/93‑1 (MQ=255)

                                                                         ttGGCGGTGCCATTGGTGCGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTg                <  1:3076791/93‑1 (MQ=255)

                                                                          tGGCGGTGCCATTGGTGCGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTgg               >  2:908702/1‑93 (MQ=255)

                                                                          tGGCGGTGCCATTGGTGCGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTgg               <  1:4277958/93‑1 (MQ=255)

                                                                          tGGCGGTGCCATTGGTGCGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTgg               <  1:6106156/93‑1 (MQ=255)

                                                                           ggCGGTGCCATTGGTGCGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGt              <  2:3451797/93‑1 (MQ=255)

                                                                           ggCGGTGCCATTGGTGCGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGt              <  2:2405717/93‑1 (MQ=255)

                                                                            gCGGTGCCATTGGTGCGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGtt             >  1:5645060/1‑93 (MQ=255)

                                                                              ggTGCCATTGGTGCGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGtttt           >  2:6137980/1‑93 (MQ=255)

                                                                               gggCCATTGGTGCGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTa          <  2:1623909/91‑1 (MQ=255)

                                                                               gTGCCATTGGTGCGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTa          <  1:2610607/93‑1 (MQ=255)

                                                                               gTGCCATTGGTGCGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTa          <  1:2684509/93‑1 (MQ=255)

                                                                               gTGCCATGGGTGCGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTa          <  2:5546397/93‑1 (MQ=255)

                                                                                tGCCATTGGTGCGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTAt         <  1:3509184/93‑1 (MQ=255)

                                                                                  ccATTGGTGCGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGc       >  2:2809908/1‑93 (MQ=255)

                                                                                   cATTGGTGCGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGct      >  1:5658153/1‑93 (MQ=255)

                                                                                   cATTGGTGCGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGct      >  1:3495279/1‑93 (MQ=255)

                                                                                    aTTGGTGCGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGctt     >  1:1525954/1‑92 (MQ=255)

                                                                                     ttGGTGCGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGctct    >  1:4369906/1‑93 (MQ=255)

                                                                                     ttGGTGCGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGctct    >  2:3526654/1‑93 (MQ=255)

                                                                                      tGGTGCGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCtt   <  1:2894693/93‑1 (MQ=255)

GCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTT  >  NC_000913/4427466‑4427645

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

GTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTTTTCG  >  NC_000913/4427463‑4427649

GTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTG                                                                                               <  SRR4302533.2852321/94‑1 (MQ=60)

GTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTG                                                                                               >  SRR4302533.2743136/1‑94 (MQ=60)

GTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTG                                                                                               <  SRR4302533.5576467/94‑1 (MQ=60)

  TGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTGCG                                                                                             >  SRR4302533.4612412/1‑94 (MQ=60)

  TGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTGCG                                                                                             <  SRR4302533.710159/94‑1 (MQ=60)

     CGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTGCGGGG                                                                                          >  SRR4302533.5327795/1‑94 (MQ=60)

     CGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTGCGGGG                                                                                          <  SRR4302533.125028/94‑1 (MQ=60)

      GATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGCCATATTCAGCTTATTGCCATTGGCGGGGCCATTGGCGCGGGGT                                                                                         >  SRR4302533.65438/1‑94 (MQ=60)

      GATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTGCGGGGT                                                                                         >  SRR4302533.5306996/1‑94 (MQ=60)

       ATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTGCGGGGTT                                                                                        >  SRR4302533.2594696/1‑94 (MQ=60)

       ATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTGCGGGGTT                                                                                        >  SRR4302533.4248539/1‑94 (MQ=60)

        TGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTGCGGGGTTG                                                                                       >  SRR4302533.4984145/1‑94 (MQ=60)

        TGATCAGGCTCCGACTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTGCGGGGTTG                                                                                       <  SRR4302533.3526250/94‑1 (MQ=60)

         GATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTGCGGGGTTGT                                                                                      >  SRR4302533.815305/1‑94 (MQ=60)

          ATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTGCGGGGTTGTT                                                                                     >  SRR4302533.6214871/1‑94 (MQ=60)

           TCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTGCGGGGTTGTTT                                                                                    <  SRR4302533.4557693/94‑1 (MQ=60)

            CAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTGCGGGGTTGTTTA                                                                                   <  SRR4302533.1674732/94‑1 (MQ=60)

            CAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTGCGGGGTTGTTTA                                                                                   <  SRR4302533.5006466/94‑1 (MQ=60)

            CAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTGCGGGGTTGTTTA                                                                                   >  SRR4302533.3362755/1‑94 (MQ=60)

             AGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTGCGGGGTTGTTTAT                                                                                  <  SRR4302533.3894857/94‑1 (MQ=60)

             AGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTGCGGGGTTGTTTAT                                                                                  <  SRR4302533.2103695/94‑1 (MQ=60)

             AGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTGCGGGGTTGTTTAT                                                                                  <  SRR4302533.4946590/94‑1 (MQ=60)

              GGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTGCGGGGTTGTTTATG                                                                                 <  SRR4302533.1190637/94‑1 (MQ=60)

              GGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTGCGGGGTTGTTTATG                                                                                 <  SRR4302533.478836/94‑1 (MQ=60)

                 TCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTGCGGGGTTGTTTATGGGG                                                                              <  SRR4302533.2636182/94‑1 (MQ=60)

                  CCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTGCGGGGTTGTTTATGGGGT                                                                             <  SRR4302533.3173209/94‑1 (MQ=60)

                    GGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTGCGGGGTTGTTTATGGGGTCT                                                                           <  SRR4302533.4337828/94‑1 (MQ=60)

                     GCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTGCGGGGTTGTTTATGGGGTCTG                                                                          <  SRR4302533.821832/94‑1 (MQ=60)

                      CTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTGCGGGGTTGTTTATGGGGTCTGG                                                                         <  SRR4302533.3729351/94‑1 (MQ=60)

                      CTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTGCGGGGTTGTTTATGGGGTCTGG                                                                         <  SRR4302533.1157873/94‑1 (MQ=60)

                       TGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTGCGGGGTTGTTTATGGGGTCTGGC                                                                        <  SRR4302533.114085/94‑1 (MQ=60)

                       TGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTGCGGGGTTGTTTATGGGGTCTGGC                                                                        >  SRR4302533.2288620/1‑94 (MQ=60)

                        GAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTGCGGGGTTGTTTATGGGGTCTGGCA                                                                       >  SRR4302533.1824450/1‑94 (MQ=60)

                          ACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTGCGGGGTTGTTTATGGGGTCTGGCAAA                                                                     >  SRR4302533.3628390/1‑94 (MQ=60)

                            AGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTGCGGGGTTGTTTATGGGGTCTGGCAAAAC                                                                   <  SRR4302533.4327430/94‑1 (MQ=60)

                               CGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTGCGGGGTTGTTTATGGGGTCTGGCAAAACGAT                                                                <  SRR4302533.4060629/94‑1 (MQ=60)

                               CGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTGCGGGGTTGTTTATGGGGTCTGGCAAAACGAT                                                                <  SRR4302533.3953508/94‑1 (MQ=60)

                               CGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTGCGGGGTTGTTTATGGGGTCTGGCAAAACGAT                                                                <  SRR4302533.5144906/94‑1 (MQ=60)

                                 CTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTGCGGGGTTGTTTATGGGGTCTGGCAAAACGATTA                                                              <  SRR4302533.697377/94‑1 (MQ=60)

                                 CTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTGCGGGGTTGTTTATGGGGTCTGGCAAAACGATTA                                                              >  SRR4302533.1645036/1‑94 (MQ=60)

                                   ACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTGCGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGC                                                            <  SRR4302533.4803162/94‑1 (MQ=60)

                                    CGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTGCGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCC                                                           >  SRR4302533.957022/1‑94 (MQ=60)

                                     GGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTGCGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCT                                                          <  SRR4302533.5696531/94‑1 (MQ=60)

                                     GGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTGCGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCT                                                          <  SRR4302533.2918863/94‑1 (MQ=60)

                                      GCGCAATCTCACAAACCGACATATTCAGCTTAGTGCCATTGGCTGTGCCATTGGTGCGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTT                                                         <  SRR4302533.825309/94‑1 (MQ=60)

                                         CAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTGCGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCC                                                      >  SRR4302533.240459/1‑94 (MQ=60)

                                         CAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTGCGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCC                                                      <  SRR4302533.4548932/94‑1 (MQ=60)

                                          AATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTGCGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCG                                                     <  SRR4302533.2371180/94‑1 (MQ=60)

                                          AATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTGCGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCG                                                     >  SRR4302533.283613/1‑94 (MQ=60)

                                          AATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTGCGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCG                                                     >  SRR4302533.2097937/1‑94 (MQ=60)

                                           ATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTGCGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGG                                                    >  SRR4302533.4095083/1‑94 (MQ=60)

                                           ATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTGCGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGG                                                    >  SRR4302533.3363274/1‑94 (MQ=60)

                                            TCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTGCGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGG                                                   <  SRR4302533.2860232/94‑1 (MQ=60)

                                            TCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTGCGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGG                                                   <  SRR4302533.2203874/94‑1 (MQ=60)

                                            TCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTGCGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGG                                                   <  SRR4302533.1310321/94‑1 (MQ=60)

                                             CTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTGCGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGC                                                  >  SRR4302533.2393220/1‑94 (MQ=60)

                                             CTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTGCGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGC                                                  >  SRR4302533.5202347/1‑94 (MQ=60)

                                              TCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTGCGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCC                                                 <  SRR4302533.1097021/94‑1 (MQ=60)

                                              TCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTCCGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCC                                                 <  SRR4302533.4113195/94‑1 (MQ=60)

                                               CACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTGCGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCG                                                <  SRR4302533.5319953/94‑1 (MQ=60)

                                                ACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTGCGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGT                                               <  SRR4302533.4103935/94‑1 (MQ=60)

                                                ACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTGCGGGGGTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGT                                               >  SRR4302533.4326179/1‑94 (MQ=60)

                                                 CAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTGCGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTC                                              >  SRR4302533.4636084/1‑94 (MQ=60)

                                                    ACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTGCGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGAT                                           <  SRR4302533.5701754/94‑1 (MQ=60)

                                                     CCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTGCGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATC                                          <  SRR4302533.4055472/94‑1 (MQ=60)

                                                     CCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTGCGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATC                                          >  SRR4302533.4278144/1‑94 (MQ=60)

                                                     CCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTGCGGGGGTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATC                                          >  SRR4302533.1967078/1‑94 (MQ=60)

                                                      CGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTGCGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTTGATCA                                         <  SRR4302533.901394/94‑1 (MQ=60)

                                                      CGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTGCGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCA                                         >  SRR4302533.4604887/1‑94 (MQ=60)

                                                      CGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTGCGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCA                                         >  SRR4302533.1173041/1‑94 (MQ=60)

                                                      CGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTGCGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCA                                         <  SRR4302533.2966455/94‑1 (MQ=60)

                                                       GACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTGCGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCAT                                        <  SRR4302533.1792501/94‑1 (MQ=60)

                                                       GACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTGCGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCAT                                        <  SRR4302533.1731360/94‑1 (MQ=60)

                                                       GACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTGCGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCAT                                        <  SRR4302533.3267775/94‑1 (MQ=60)

                                                       GACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTGCGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCAT                                        >  SRR4302533.2307098/1‑94 (MQ=60)

                                                         CATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTGCGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTT                                      <  SRR4302533.1302224/94‑1 (MQ=60)

                                                         CATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTGCGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTT                                      >  SRR4302533.2610238/1‑94 (MQ=60)

                                                         CATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTGCGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTT                                      <  SRR4302533.4728229/94‑1 (MQ=60)

                                                         CATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTGCGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTT                                      >  SRR4302533.2205134/1‑94 (MQ=60)

                                                          ATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTGCGGGGTTGTTTTTGGGTCTTGGCAAAACAATT‑GCCCTGCCGGGCCGTCGGTCATTTTC                                    >  SRR4302533.174963/1‑94 (MQ=48)

                                                          ATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTGCGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTT                                     >  SRR4302533.237388/1‑94 (MQ=60)

                                                           TATTCAGCTTATTGCCATTGGCGGTGCCATTGGTGCGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTC                                    <  SRR4302533.2246271/94‑1 (MQ=60)

                                                           TATTCAGCTTATTGCCATTGGCGGTGCCATTGGTGCGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTC                                    <  SRR4302533.1287138/94‑1 (MQ=60)

                                                            ATTCAGCTTATTGCCATTGGCGGTGCCATTGGTGCGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCG                                   <  SRR4302533.5161947/94‑1 (MQ=60)

                                                            ATTCAGCTTATTGCCATTGGCGGTGCCATTGGTGCGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCG                                   <  SRR4302533.1886776/94‑1 (MQ=60)

                                                               CAGCTTATTGCCATTGGCGGTGCCATTGGTGCGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTT                                <  SRR4302533.832375/94‑1 (MQ=60)

                                                               CAGCTTATTGCCATTGGCGGTGCCATTGGTGCGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTT                                >  SRR4302533.4387146/1‑94 (MQ=60)

                                                                AGCTTATTGCCATTGGCGGTGCCATTGGTGCGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTA                               >  SRR4302533.2666196/1‑94 (MQ=60)

                                                                AGCTTATTGCCATTGGCGGTGCCATTGGTGCGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTA                               <  SRR4302533.773169/94‑1 (MQ=60)

                                                                 GCTTATTGCCATTGGCGGTGCCATTGGTGCGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTAT                              <  SRR4302533.1749117/94‑1 (MQ=60)

                                                                 GCTTATTGCCATTGGCGGTGCCATTGGTGCGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTAT                              <  SRR4302533.1698997/94‑1 (MQ=60)

                                                                  GTTATTGCCATTGGCGGTGCCATTGGTGCGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATA                             <  SRR4302533.2712657/94‑1 (MQ=60)

                                                                  CTTATTGCCATTGGCGGTGCCATTGGTGCGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATA                             <  SRR4302533.5356491/94‑1 (MQ=60)

                                                                  CTTATTGCCATTGGCGGTGCCATTGGTGCGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATA                             >  SRR4302533.2140198/1‑94 (MQ=60)

                                                                  CTTATTGCCATTGGCGGTGCCATTGGTGCGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATA                             <  SRR4302533.5152850/94‑1 (MQ=60)

                                                                  CTTATTGCCATTGGCGGTGCCATTGGTGCGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATA                             >  SRR4302533.2167692/1‑94 (MQ=60)

                                                                   TTATTGCCATTGGCGGTGCCATTGGTGCGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATAT                            >  SRR4302533.2443012/1‑94 (MQ=60)

                                                                   TTATTGCCATTGGCGGTGCCATTGGTGCGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATAT                            >  SRR4302533.905222/1‑94 (MQ=60)

                                                                   TTATTGCCATTGGCGGTGCCATTGGTGCGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATAT                            <  SRR4302533.3546793/94‑1 (MQ=60)

                                                                    TATTGCCATTGGCGGTGCCATTGGTGCGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTGTATG                           <  SRR4302533.265832/94‑1 (MQ=60)

                                                                    TATTGCCATTGGCGGTGCCATTGGTGCGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATG                           <  SRR4302533.4548366/94‑1 (MQ=60)

                                                                    TATTGCCATTGGCGGTGCCATTGGTGCGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATG                           <  SRR4302533.2544588/94‑1 (MQ=60)

                                                                     ATTGCCATTGGCGGTGCCATTGGTGCGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGA                          >  SRR4302533.4725551/1‑94 (MQ=60)

                                                                     ATTGCCATTGGCGGTGCCATTGGTGCGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGA                          >  SRR4302533.2502175/1‑94 (MQ=60)

                                                                        TCCATTGGCGGTGCCATTGGTGCGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCA                       <  SRR4302533.1647109/94‑1 (MQ=60)

                                                                        GCCATTGGCGGTGCCATTGGTGCGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCT                       >  SRR4302533.3337751/1‑94 (MQ=60)

                                                                        GCCATTGGCGGTGCCATTGGTGCGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCA                       <  SRR4302533.2643158/94‑1 (MQ=60)

                                                                         CCATTGGCGGTGCCATTGGTGCGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCAT                      <  SRR4302533.2582597/94‑1 (MQ=60)

                                                                          CATTGGCGGTGCCATTGGTGCGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATT                     <  SRR4302533.4832530/94‑1 (MQ=60)

                                                                          CATTGGCGGTGCCATTGGTGCGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATT                     <  SRR4302533.5435210/94‑1 (MQ=60)

                                                                          CATTGGCGGTGCCATTGGTGCGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATT                     >  SRR4302533.2863284/1‑94 (MQ=60)

                                                                           ATTGGCGGTGCCATTGGTGCGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTG                    <  SRR4302533.5000962/94‑1 (MQ=60)

                                                                            TTGGCGGTGCCATTGGTGCGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTGGATCATTTTCGTTTATATGATCATTGG                   >  SRR4302533.3478299/1‑94 (MQ=60)

                                                                            TTGGCGGTGCCATTGGTGCGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGG                   <  SRR4302533.3095674/94‑1 (MQ=60)

                                                                             TGGCGGTGCCATTGGTGCGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGT                  <  SRR4302533.6143027/94‑1 (MQ=60)

                                                                             TGGCGGTGCCATTGGTGCGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGT                  <  SRR4302533.4591063/94‑1 (MQ=60)

                                                                             TGGCGGTGCCATTGGTGCGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGT                  <  SRR4302533.4303683/94‑1 (MQ=60)

                                                                              GGCGGTGCCATTGGTGCGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTT                 <  SRR4302533.1108534/94‑1 (MQ=60)

                                                                              GGCGGTGCCATTGGTGCGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTT                 >  SRR4302533.5679186/1‑94 (MQ=60)

                                                                              GGCGGTGCCATTGGTGCGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTT                 <  SRR4302533.1317535/94‑1 (MQ=60)

                                                                                CGGTGCCATTGGTGCGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTT               >  SRR4302533.4805040/1‑94 (MQ=60)

                                                                                  GTGCCATTGGTGCGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTAT             <  SRR4302533.2626631/94‑1 (MQ=60)

                                                                                  GTGCCATTGGTGCGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTAT             <  SRR4302533.2700959/94‑1 (MQ=60)

                                                                                   TGCCATTGGTGCGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATG            <  SRR4302533.3530819/94‑1 (MQ=60)

                                                                                    GCCATTGGTGCGGGGGTGTTTATGGGGTCTGGGAAAACGATTAGCCTTGCCGGGCCGCAGATCAGTTTCGTTGATATGATCATTGGTTTTATGC           >  SRR4302533.2551412/1‑94 (MQ=60)

                                                                                     CCATTGGTGCGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCT          >  SRR4302533.5692344/1‑94 (MQ=60)

                                                                                     CCATTGGTGCGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCT          >  SRR4302533.2312711/1‑94 (MQ=60)

                                                                                     CCATTGGTGCGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCT          >  SRR4302533.3516837/1‑94 (MQ=60)

                                                                                      CATTGGTGCGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTT         >  SRR4302533.1535138/1‑94 (MQ=60)

                                                                                      CATTGGTGCGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTC         <  SRR4302533.3784457/94‑1 (MQ=60)

                                                                                      CATTGGTGCGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTC         >  SRR4302533.596283/1‑94 (MQ=60)

                                                                                      CATTGGTGCGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTC         <  SRR4302533.5686045/94‑1 (MQ=60)

                                                                                       ATTGGTGCGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCT        >  SRR4302533.4396289/1‑94 (MQ=60)

                                                                                        TTGGTGCGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTT       <  SRR4302533.4152184/94‑1 (MQ=60)

                                                                                         TGGTGCGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTT      <  SRR4302533.2912479/94‑1 (MQ=60)

                                                                                         TGGTGCGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTT      <  SRR4302533.5700580/94‑1 (MQ=60)

                                                                                          GGTGCGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTTT     <  SRR4302533.1493594/94‑1 (MQ=60)

                                                                                          GGTGCGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTTT     <  SRR4302533.5456207/94‑1 (MQ=60)

                                                                                           GTGCGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTTTT    >  SRR4302533.3554229/1‑94 (MQ=60)

                                                                                            TGCGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTTTTC   <  SRR4302533.5574202/94‑1 (MQ=60)

                                                                                            TGCGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTTTTC   <  SRR4302533.5201611/94‑1 (MQ=60)

                                                                                             GCGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTTTTCG  >  SRR4302533.2833358/1‑94 (MQ=60)

                                                                                             GCGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTTTTCG  <  SRR4302533.2078308/94‑1 (MQ=60)

GTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTTTTCG  >  NC_000913/4427463‑4427649

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`