Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1624	250.6	12620048	98.0%	12367647	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,431,142	T→C	H154R (CAC→CGC)	ytfG ←	putative oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,431,142	0	T	C	100.0%	805.8 / NA	253	H154R (CAC→CGC)	ytfG	putative oxidoreductase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (132/121); total (132/121)

Only 100 of 274 total aligned reads displayed.

TCGCTAATCACGCGTGCCGCAGCTGCCGCATAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGTGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAGGGTGTAAACGATGCCAGAATCAGCCAGC  >  NC_000913/4431052‑4431231

tCGCTAATCACGCGTGCCGCAGCTGCCGCATAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCgcgt                                                                                         >  2:3494359/1‑93 (MQ=255)

tCGCTAATCACGCGTGCCGCAGCTGCCGCATAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCgcgt                                                                                         >  2:3250830/1‑93 (MQ=255)

 cGCTAATCACGCGTCCCGCAGCTGCCGCATAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGtt                                                                                        <  2:3764797/93‑1 (MQ=255)

   cTAATCACGCGTGCCGCAGCTGCCGCATAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTcc                                                                                      >  2:5977929/1‑93 (MQ=255)

   cTAATCACGCGTGCCGCAGCTGCCGCATAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTcc                                                                                      >  2:2107150/1‑93 (MQ=255)

    tAATCACGCGTGCCGCAGCTGCCGCATAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCa                                                                                     <  2:4083534/93‑1 (MQ=255)

     aaTCACGCGTGCCGCAGCTGCCGCATAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAg                                                                                    <  1:3822772/93‑1 (MQ=255)

     aaTCACGCGTGCCGCAGCTGCCGCATAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAg                                                                                    <  1:2157598/93‑1 (MQ=255)

      aTCACGCGTGCCGCAGCTGCCGCATAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGt                                                                                   >  1:5689031/1‑93 (MQ=255)

       tCACGCGTGCCGCAGCTGCCGCATAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGtg                                                                                  <  2:382782/93‑1 (MQ=255)

       tCACGCGTGCCGCAGCTGCCGCATAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGtg                                                                                  >  2:2581508/1‑93 (MQ=255)

       tCACGCGTGCCGCAGCTGCCGCATAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGtg                                                                                  <  2:3680461/93‑1 (MQ=255)

        cACGCGTGCCGCAGCTGCCGCATAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGtgc                                                                                 >  2:6208659/1‑93 (MQ=255)

        cACGCGGCCCGCAGCTGCCGCATAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGtgc                                                                                 <  2:5448169/93‑1 (MQ=255)

           gcgTGCCGCAGCTGCCGCATAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGtgctgc                                                                              >  2:690708/1‑93 (MQ=255)

           gcgTGCCGCAGCTGCCGCATAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGACGCGTTCCAGtgctgc                                                                              >  1:5822742/1‑93 (MQ=255)

           gcgTGCCGCAGCCGCCGCATAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGtgctgc                                                                              <  2:5433187/93‑1 (MQ=255)

            cgTGCCGCAGCTGCCGCATAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGcc                                                                             <  2:2297920/93‑1 (MQ=255)

             gTGCCGCAGCTGCCGCATAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCg                                                                            <  2:4942055/93‑1 (MQ=255)

             gTGCCGCAGCTGCCGCATAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCg                                                                            <  1:1424183/93‑1 (MQ=255)

               gCCGCAGCTGCCGCATAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCggg                                                                          <  1:4455913/93‑1 (MQ=255)

               gCCGCAGCTGCCGCATAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCggg                                                                          >  2:2911035/1‑93 (MQ=255)

                ccGCAGCTGCCGCATAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCgggg                                                                         <  2:3720775/93‑1 (MQ=255)

                ccGCAGCTGCCGCATAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCgggg                                                                         <  2:6285617/93‑1 (MQ=255)

                ccGCAGCTGCCGCATAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCgggg                                                                         <  2:270871/93‑1 (MQ=255)

                  gCAGCTGCCGCATAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGgcg                                                                       >  1:259304/1‑93 (MQ=255)

                    aGCTGCCGCATAATCTGCCCGCGTTGCTGCGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCt                                                                     >  1:3166709/1‑93 (MQ=255)

                    aGCTGCCGCATAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCt                                                                     <  1:1066500/93‑1 (MQ=255)

                    aGCTGCCGCATAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCt                                                                     <  1:5542728/93‑1 (MQ=255)

                    aGCTGCCGCATAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCt                                                                     >  2:4880509/1‑93 (MQ=255)

                    aGCTGCCGCATAATCTGCCCGCGTTGCTGAGGCGATTTTGCCAGCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCGGGGGCGCt                                                                     <  2:4368711/93‑1 (MQ=255)

                     gCTGCCGCATAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTg                                                                    >  2:3319013/1‑93 (MQ=255)

                     gCTGCCGCATAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTg                                                                    >  1:1877565/1‑93 (MQ=255)

                     gCTGCCGCATAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTg                                                                    >  2:1456768/1‑93 (MQ=255)

                     gCTGCCGCATAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTg                                                                    >  1:3340580/1‑93 (MQ=255)

                      cTGCCGCATAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTgg                                                                   <  2:1422691/93‑1 (MQ=255)

                      cTGCCGCATAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTgg                                                                   <  2:5379652/93‑1 (MQ=255)

                         ccGCATAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGa                                                                <  1:4843354/93‑1 (MQ=255)

                         ccGCATAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGa                                                                >  1:4296175/1‑93 (MQ=255)

                         ccGCATAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGa                                                                <  1:3146245/93‑1 (MQ=255)

                         ccGCATAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGa                                                                <  1:878983/93‑1 (MQ=255)

                           gCATAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAgg                                                              >  2:1552580/1‑93 (MQ=255)

                            cATAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGt                                                             >  2:358885/1‑93 (MQ=255)

                            cATAACCGGCCCGCGTGGCTGAGGCGTGTGTGCCACCGCCCGCCGCACCGATAAATGCGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGt                                                             <  2:1154004/93‑1 (MQ=255)

                             aTAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTa                                                            <  2:286426/93‑1 (MQ=255)

                             aTAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTa                                                            >  2:242799/1‑93 (MQ=255)

                             aTAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTa                                                            <  2:623383/93‑1 (MQ=255)

                              tAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAg                                                           >  1:2448814/1‑93 (MQ=255)

                              tAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAg                                                           <  1:6022365/93‑1 (MQ=255)

                              tAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAg                                                           >  2:3896262/1‑93 (MQ=255)

                              tAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAg                                                           >  1:5342516/1‑93 (MQ=255)

                               aaTCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGt                                                          >  1:5031526/1‑93 (MQ=255)

                               aaTCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGGAGt                                                          >  1:4632704/1‑93 (MQ=255)

                                aTCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGtt                                                         <  1:2070180/93‑1 (MQ=255)

                                 tCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGttt                                                        >  2:2761778/1‑93 (MQ=255)

                                   tGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTc                                                      >  1:4254967/1‑93 (MQ=255)

                                   tGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTc                                                      >  2:4992178/1‑93 (MQ=255)

                                   tGCCCGCGTGCCTGAGGCGTTTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTc                                                      <  1:4136375/93‑1 (MQ=255)

                                      ccGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCt                                                   <  1:150426/93‑1 (MQ=255)

                                      ccGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCt                                                   <  1:2350455/93‑1 (MQ=255)

                                        gcgTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCTGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGt                                                 >  1:2080233/1‑93 (MQ=255)

                                        gcgTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGt                                                 <  2:5305913/93‑1 (MQ=255)

                                         cgTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTa                                                <  1:5584946/93‑1 (MQ=255)

                                          gTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTAc                                               >  1:2683490/1‑93 (MQ=255)

                                           ttGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTAcc                                              <  1:3692584/93‑1 (MQ=255)

                                           ttGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCAAGTGCTGCCGGGGCGCTGCCGAGGTGGTTTTCGCTGTAcc                                              >  2:1105561/1‑93 (MQ=255)

                                           ttGCGGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTAcc                                              <  2:3529977/93‑1 (MQ=255)

                                            tGCTGAGGCGATTTTGCTATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCa                                             <  1:2785398/93‑1 (MQ=255)

                                             gCTGAGGCGATTTTCCCACCCCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAg                                            <  1:798951/93‑1 (MQ=255)

                                                gAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCg                                         >  1:1732322/1‑93 (MQ=255)

                                                 aGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGt                                        >  1:4936554/1‑93 (MQ=255)

                                                  ggCCATGTTGTCATCCCCCGCCCCACCAATAAAGACGCGGCGTACAAGGGTTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGtt                                       <  2:3903370/93‑1 (MQ=255)

                                                   gCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTg                                      <  2:4613947/93‑1 (MQ=255)

                                                    cGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTgc                                     >  1:113859/1‑93 (MQ=255)

                                                    cGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTgc                                     <  2:4692348/93‑1 (MQ=255)

                                                      aTTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTgcgc                                   <  1:6104683/93‑1 (MQ=255)

                                                           gCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGcagcag                              <  1:672543/93‑1 (MQ=255)

                                                            ccATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAgg                             >  2:3146841/1‑93 (MQ=255)

                                                             cATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAggg                            <  1:4776586/93‑1 (MQ=255)

                                                                 gcccgccGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAGGGTGTa                        >  1:2629305/1‑93 (MQ=255)

                                                                 gcccgccGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAGGGTGTa                        >  2:3267305/1‑93 (MQ=255)

                                                                 gcccgccGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAGGGTGTa                        <  1:3057970/93‑1 (MQ=255)

                                                                   ccgccgCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAGGGTGTaaa                      <  1:3927808/93‑1 (MQ=255)

                                                                     gccgcACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAGGGTGTAAACg                    >  2:1056375/1‑93 (MQ=255)

                                                                       cgcACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAGGGTGTAAACGAt                  <  1:4100050/93‑1 (MQ=255)

                                                                        ccACCTAAAAATACGCCGCGTTCCAGTGCGGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAGGGTGTAAACGATg                 <  2:1730119/92‑1 (MQ=255)

                                                                         cACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAGGGTGTAAACGATGc                <  1:3270605/93‑1 (MQ=255)

                                                                          aCCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAGGGTGTAAACGATGcc               <  2:2221835/93‑1 (MQ=255)

                                                                            cGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAGGGTGTAAACGATGCCAg             <  2:4532510/93‑1 (MQ=255)

                                                                            cGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAGGGTGTAAACGATGCCAg             <  2:5559906/93‑1 (MQ=255)

                                                                            cGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAGGGTGTAAACGATGCCAg             >  1:524725/1‑93 (MQ=255)

                                                                             gATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAGGGTGTAAACGATGCCAGa            >  1:926077/1‑93 (MQ=255)

                                                                                aaaTACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAGGGTGTAAACGATGCCAGAATc         <  2:3065852/93‑1 (MQ=255)

                                                                                  aTACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAGGGTGTAAACGATGACAGAATcag       >  2:1965937/1‑93 (MQ=255)

                                                                                  aTACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGGAGTTTTCGCTGTACCAGCCGTTGCGCAGCAGGGTGTAAACGATGCGAGAATcag       >  1:5404696/1‑93 (MQ=255)

                                                                                   tACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAGGGTGTAAACGATGCCAGAATcagc      <  1:4567141/93‑1 (MQ=255)

                                                                                   tACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAGGGTGTAAACGATGCCAGAATcagc      >  1:4660617/1‑93 (MQ=255)

                                                                                    aCGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAGGGTGTAAACGATGCCAGAATcagcc     <  1:1775031/93‑1 (MQ=255)

                                                                                       ccGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAGGGTGTAAACGATGCCAGAATcagccagc  <  1:2942460/93‑1 (MQ=255)

                                                                                       ccGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAGGGTGTAAACGATGCCAGAATcagccagc  <  2:4308315/93‑1 (MQ=255)

TCGCTAATCACGCGTGCCGCAGCTGCCGCATAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGTGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAGGGTGTAAACGATGCCAGAATCAGCCAGC  >  NC_000913/4431052‑4431231

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

GCTTCGCTAATCACGCGTGCCGCAGCTGCCGCATAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGTGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAGGGTGTAAACGATGCCAGAATCAGCCAGCATTT  >  NC_000913/4431049‑4431235

GCTTCGCTAATCACGCGTGCCGCAGCTGCCGCATAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGC                                                                                               <  SRR4302533.666113/94‑1 (MQ=60)

 CTTCGCTAATCACGCGTGCCGCAGCTGCCGCATAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCG                                                                                              >  SRR4302533.2235511/1‑94 (MQ=60)

  TTCGCTAATCACGCGTGCCGCAGCTGCCGCATAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGT                                                                                             >  SRR4302533.709146/1‑94 (MQ=60)

    CGCTAATCACGCGTGCCGCAGCTGCCGCATAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTC                                                                                           >  SRR4302533.3183527/1‑94 (MQ=60)

      CTAATCACGCGTGCCGCAGCTGCCGCATAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCA                                                                                         >  SRR4302533.4813476/1‑94 (MQ=60)

       TAATCACGCGTGCCGCAGCTGCCGCATAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAG                                                                                        >  SRR4302533.4301742/1‑94 (MQ=60)

        AATCACGCGTGCCGCAGCTGCCGCATAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGT                                                                                       <  SRR4302533.3846143/94‑1 (MQ=60)

        AATCACGCGTGCCGCAGCTGCCGCATAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGT                                                                                       >  SRR4302533.5723393/1‑94 (MQ=60)

        AATCACGCGTGCCGCAGCTGCCGCATAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGT                                                                                       <  SRR4302533.2170880/94‑1 (MQ=60)

        AAGCACGCGTGCCGCAGCTGCCGCATAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGT                                                                                       <  SRR4302533.2427008/94‑1 (MQ=60)

           CACGCGTGCCGCAGCTGCCGCATAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCT                                                                                    <  SRR4302533.901810/94‑1 (MQ=60)

             CGCGTGCCGCAGCTGCCGCATAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGACGCGTTCCAGTGCTGC                                                                                  >  SRR4302533.5857912/1‑94 (MQ=60)

               CGTGCCGCAGCTGCCGCATAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCG                                                                                <  SRR4302533.3416453/94‑1 (MQ=60)

                GTGCCGCAGCTGCCGCATAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGG                                                                               <  SRR4302533.1432798/94‑1 (MQ=60)

                  GCCGCAGCTGCCGCATAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGG                                                                             >  SRR4302533.3032732/1‑94 (MQ=60)

                  GCCGCAGCTGCCGCATAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGG                                                                             <  SRR4302533.271943/94‑1 (MQ=60)

                  GCCGCAGCTGCCGCATAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGG                                                                             <  SRR4302533.4482858/94‑1 (MQ=60)

                   CCGCAGCTGCCGCATAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGC                                                                            <  SRR4302533.1754802/94‑1 (MQ=60)

                    CGCAGCTGCCGCATAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCG                                                                           >  SRR4302533.260879/1‑94 (MQ=60)

                    CGCAGCTGCCGCATAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCG                                                                           <  SRR4302533.1836601/94‑1 (MQ=60)

                    CGCAGCTGCCGCATAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCG                                                                           >  SRR4302533.5027529/1‑94 (MQ=60)

                     GCAGCTGCCGCATAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGC                                                                          <  SRR4302533.4544480/94‑1 (MQ=60)

                      CAGCTGCCGCATAATCTGCCCGCGTTGCTGCGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCT                                                                         >  SRR4302533.3186194/1‑94 (MQ=60)

                      CAGCTGCCGCATAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCT                                                                         <  SRR4302533.4376957/94‑1 (MQ=60)

                       AGCTGCCGCATAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTG                                                                        >  SRR4302533.1889009/1‑94 (MQ=60)

                       AGCTGCCGCATAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTG                                                                        <  SRR4302533.1072766/94‑1 (MQ=60)

                       AGCTGCCGCATAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTG                                                                        >  SRR4302533.3361226/1‑94 (MQ=60)

                       AGCTGCCGCATAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTG                                                                        <  SRR4302533.5576203/94‑1 (MQ=60)

                        GCTGCCGCATAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGG                                                                       <  SRR4302533.4316909/94‑1 (MQ=60)

                        GCTGCCGCATAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGG                                                                       >  SRR4302533.847320/1‑94 (MQ=60)

                         CTGCCGCATAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGC                                                                      <  SRR4302533.4057614/94‑1 (MQ=60)

                           GCCGCATAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGA                                                                    >  SRR4302533.4322013/1‑94 (MQ=60)

                            CCGCATAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAG                                                                   <  SRR4302533.4872709/94‑1 (MQ=60)

                            CCGCATAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAG                                                                   <  SRR4302533.3165592/94‑1 (MQ=60)

                            CCGCATAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAG                                                                   <  SRR4302533.884142/94‑1 (MQ=60)

                            CCGCATAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAG                                                                   >  SRR4302533.631772/1‑94 (MQ=60)

                            CCGCATAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAG                                                                   >  SRR4302533.2652127/1‑94 (MQ=60)

                            CCGCATAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAG                                                                   >  SRR4302533.5856232/1‑94 (MQ=60)

                            CCGCATAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAG                                                                   <  SRR4302533.3166586/94‑1 (MQ=60)

                            CCGCATAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAG                                                                   <  SRR4302533.412524/94‑1 (MQ=60)

                             CGCATAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGG                                                                  >  SRR4302533.5658806/1‑94 (MQ=60)

                             CGCATAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGG                                                                  >  SRR4302533.3043995/1‑94 (MQ=60)

                              GCATAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGT                                                                 >  SRR4302533.221614/1‑94 (MQ=60)

                               CATAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTA                                                                >  SRR4302533.1754742/1‑94 (MQ=60)

                                ATAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAG                                                               >  SRR4302533.5374555/1‑94 (MQ=60)

                                ATAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAG                                                               >  SRR4302533.2463929/1‑94 (MQ=60)

                                ATAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAG                                                               >  SRR4302533.195333/1‑94 (MQ=60)

                                 TAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGT                                                              >  SRR4302533.5061991/1‑94 (MQ=60)

                                 TAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGT                                                              <  SRR4302533.4391517/94‑1 (MQ=60)

                                 TAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGT                                                              <  SRR4302533.6058861/94‑1 (MQ=60)

                                 TAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGGAGT                                                              >  SRR4302533.4660778/1‑94 (MQ=60)

                                   ATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTT                                                            <  SRR4302533.2082930/94‑1 (MQ=60)

                                   ATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTT                                                            >  SRR4302533.6290596/1‑94 (MQ=60)

                                     CTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTC                                                          >  SRR4302533.4280623/1‑94 (MQ=60)

                                      TGCCCGCGTGCCTGAGGCGTTTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCG                                                         <  SRR4302533.4161479/94‑1 (MQ=60)

                                         CCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTG                                                      <  SRR4302533.151391/94‑1 (MQ=60)

                                         CCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTG                                                      <  SRR4302533.2364996/94‑1 (MQ=60)

                                         CCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTG                                                      <  SRR4302533.1488287/94‑1 (MQ=60)

                                          CGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCTGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGT                                                     >  SRR4302533.2093031/1‑94 (MQ=60)

                                          CGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGT                                                     >  SRR4302533.5263409/1‑94 (MQ=60)

                                          CGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGT                                                     >  SRR4302533.5469192/1‑94 (MQ=60)

                                          CGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGT                                                     >  SRR4302533.5072766/1‑94 (MQ=60)

                                            CGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTAC                                                   >  SRR4302533.4664168/1‑94 (MQ=60)

                                            CGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTAC                                                   <  SRR4302533.5618692/94‑1 (MQ=60)

                                            CGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTAC                                                   >  SRR4302533.2699934/1‑94 (MQ=60)

                                              TTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCA                                                 <  SRR4302533.3715270/94‑1 (MQ=60)

                                              TTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCA                                                 <  SRR4302533.4221942/94‑1 (MQ=60)

                                              TTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCA                                                 >  SRR4302533.914714/1‑94 (MQ=60)

                                               TGCTGAGGCGATTTTGCTATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAG                                                <  SRR4302533.2802513/94‑1 (MQ=60)

                                               TGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAG                                                <  SRR4302533.4752123/94‑1 (MQ=60)

                                                GCTGAGGCGATTTTCCCACCCCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGC                                               <  SRR4302533.803654/94‑1 (MQ=60)

                                                 CTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCC                                              <  SRR4302533.3859826/94‑1 (MQ=60)

                                                 CTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCC                                              <  SRR4302533.6140226/94‑1 (MQ=60)

                                                  TGAGGCTATTTTGCAACCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCG                                             <  SRR4302533.3359406/94‑1 (MQ=60)

                                                  TGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCG                                             >  SRR4302533.1742824/1‑94 (MQ=60)

                                                   GAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGT                                            >  SRR4302533.4966444/1‑94 (MQ=60)

                                                    AGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTT                                           >  SRR4302533.5705673/1‑94 (MQ=60)

                                                    AGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTT                                           <  SRR4302533.3086732/94‑1 (MQ=60)

                                                     GGCGATTTTGCCATCGCCCGCCCCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTG                                          >  SRR4302533.180764/1‑94 (MQ=60)

                                                      GCGTTTTTGCCATCGCCGCCCGCACCGATAAATACGCCGCTTTCCAGTGCTGCCGGGGCGCTGGCGAGGTATTTTTCGCTGTTCCAGCCGTTGC                                         <  SRR4302533.4957791/94‑1 (MQ=60)

                                                      GCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGC                                         >  SRR4302533.114582/1‑94 (MQ=60)

                                                        GATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGC                                       >  SRR4302533.2394762/1‑94 (MQ=60)

                                                         ATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCA                                      <  SRR4302533.6018308/94‑1 (MQ=60)

                                                         ATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCA                                      <  SRR4302533.6141529/94‑1 (MQ=60)

                                                          TTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAG                                     <  SRR4302533.5282628/94‑1 (MQ=60)

                                                             TGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAG                                  <  SRR4302533.3298484/94‑1 (MQ=60)

                                                              GCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAGG                                 <  SRR4302533.676515/94‑1 (MQ=60)

                                                              GCAATCCCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAGG                                 <  SRR4302533.3576170/94‑1 (MQ=60)

                                                               CCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAGGG                                <  SRR4302533.5033905/94‑1 (MQ=60)

                                                                CATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAGGGT                               >  SRR4302533.2674655/1‑94 (MQ=60)

                                                                CATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAGGGT                               <  SRR4302533.4805523/94‑1 (MQ=60)

                                                                CATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAGGGG                               >  SRR4302533.2614218/1‑94 (MQ=60)

                                                                 ATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAGGGTG                              >  SRR4302533.3090627/1‑94 (MQ=60)

                                                                 ATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAGGGTG                              >  SRR4302533.852597/1‑94 (MQ=60)

                                                                   CGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAGGGTGTA                            >  SRR4302533.2645460/1‑94 (MQ=60)

                                                                   CGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAGGGTGTA                            <  SRR4302533.5375172/94‑1 (MQ=60)

                                                                   CGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAGGGTGTA                            >  SRR4302533.4862287/1‑94 (MQ=60)

                                                                   CGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAGGGTGTA                            >  SRR4302533.468035/1‑94 (MQ=60)

                                                                   CACCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGCTTTCGCTGTACCAGCCGTTGCGCAGCAGGGTGTA                            >  SRR4302533.5368904/1‑94 (MQ=60)

                                                                    GCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAGGGTGTAA                           <  SRR4302533.2801999/94‑1 (MQ=60)

                                                                    GCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAGGGTGTAA                           <  SRR4302533.3076734/94‑1 (MQ=60)

                                                                     CCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAGGGTGTAAA                          <  SRR4302533.2289004/94‑1 (MQ=60)

                                                                      CCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAGGGTGTAAAC                         <  SRR4302533.253509/94‑1 (MQ=60)

                                                                      CCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAGGGTGTAAAC                         >  SRR4302533.5612033/1‑94 (MQ=60)

                                                                      CCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAGGGTGTAAAC                         <  SRR4302533.3951788/94‑1 (MQ=60)

                                                                       CGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAGGGTGTAAACG                        >  SRR4302533.4726643/1‑94 (MQ=60)

                                                                         CCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAGGGTGTAAACGAT                      <  SRR4302533.12448/94‑1 (MQ=60)

                                                                          CGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAGGGTGTAAACGATG                     <  SRR4302533.4125002/94‑1 (MQ=60)

                                                                           GCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAGGGTGTAAACGATGC                    >  SRR4302533.3759355/1‑94 (MQ=60)

                                                                            CACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAGGGTGTAAACGATGCC                   <  SRR4302533.4555308/94‑1 (MQ=60)

                                                                            CACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAGGGTGTAAACGATGCC                   <  SRR4302533.4138263/94‑1 (MQ=60)

                                                                            CACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAGGGTGTAAACGATGCC                   <  SRR4302533.3290789/94‑1 (MQ=60)

                                                                             ACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAGGGTGTAAACGATGCCA                  <  SRR4302533.3756319/94‑1 (MQ=60)

                                                                             ACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAGGGTGTAAACGATGCCA                  >  SRR4302533.6090064/1‑94 (MQ=60)

                                                                             ACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAGGGTGTAAACGATGCCA                  <  SRR4302533.3239790/94‑1 (MQ=60)

                                                                              CCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAGGGTGTAAACGATGCCAG                 >  SRR4302533.527834/1‑94 (MQ=60)

                                                                               CGATAAATACGCCGCGTTCTAGTGCTGCCGGGGCGCTGGCGAGGTCGTTTTCGCTGTACCAGCCGTTGCGCAGCAGGGTGTAAACGATGCCAGA                >  SRR4302533.5596710/1‑94 (MQ=60)

                                                                               CGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAGGGTGTAAACGATGCCAGA                >  SRR4302533.931512/1‑94 (MQ=60)

                                                                               CGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAGGGTGTAAACGATGCCAGA                >  SRR4302533.4645805/1‑94 (MQ=60)

                                                                                GATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTGGCTGTACCAGCCGTTGCGCAGCAGGGTGTAAACGATGCCAGAA               >  SRR4302533.1506684/1‑94 (MQ=60)

                                                                                GATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAGGGTGTAAACGATGCCAGAA               >  SRR4302533.2611422/1‑94 (MQ=60)

                                                                                   AAATACGCCGCGTTCGAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAGGGTGTAAACGATGCCAGAATCA            <  SRR4302533.1932036/94‑1 (MQ=60)

                                                                                   AAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAGGGTGTAAACGATGCCAGAATCA            >  SRR4302533.6028612/1‑94 (MQ=60)

                                                                                    AATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAGGGTGTAAACGATGCCAGAATCAG           <  SRR4302533.1098020/94‑1 (MQ=60)

                                                                                    AATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAGGGTGTAAACGATGCCAGAATCAG           <  SRR4302533.2087838/94‑1 (MQ=60)

                                                                                    AATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAGGGTGTAAACGATGCCAGAATCAG           >  SRR4302533.2093464/1‑94 (MQ=60)

                                                                                    AATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGGAGTTTTCGCTGTACCAGCCGTTGCGCAGCAGGGTGTAAACGATGCGAGAATCAG           >  SRR4302533.5437173/1‑94 (MQ=60)

                                                                                     ATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAGGGTGTAAACGATGCCAGAATCAGC          >  SRR4302533.4688861/1‑94 (MQ=60)

                                                                                     ATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCGGTACCAGCCGTTGCGCAGCAGGGTGTAAACGATGCCAGAATCAGC          <  SRR4302533.2150771/94‑1 (MQ=60)

                                                                                     ATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTCCGCTGTACCAGCCGTTGCGCAGCAGGGTGTAAACGATGCCAGAATCAGC          <  SRR4302533.123296/94‑1 (MQ=60)

                                                                                      TACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAGGGTGTAAACGATGCCAGAATCAGCC         >  SRR4302533.4004891/1‑94 (MQ=60)

                                                                                      TACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAGGGTGTAAACGATGCCAGAATCAGCC         <  SRR4302533.4594828/94‑1 (MQ=60)

                                                                                       ACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAGGGTGTAAACGATGCCAGAATCAGCCA        <  SRR4302533.1785841/94‑1 (MQ=60)

                                                                                        CGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAGGGTGTAAACGATGCCAGAATCAGCCAG       >  SRR4302533.2857866/1‑94 (MQ=60)

                                                                                        CGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAGGGTGTAAACGATGCCAGAATCAGCCAG       <  SRR4302533.1569419/94‑1 (MQ=60)

                                                                                        CGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAGGGTGTAAACGATGCCAGAATCAGCCAG       >  SRR4302533.5533673/1‑94 (MQ=60)

                                                                                          CCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAGGGTGTAAACGATGCCAGAATCAGCCAGCA     <  SRR4302533.2960426/94‑1 (MQ=60)

                                                                                          CCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAGGGTGTAAACGATGCCAGAATCAGCCAGCA     >  SRR4302533.788857/1‑94 (MQ=60)

                                                                                           CGCGTTCCATTGCTGCGGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAGGGTGTAAACGATGCCAGAATCAGCCAGCAT    <  SRR4302533.4466082/94‑1 (MQ=60)

                                                                                            GCGTTCCAGTGCGTCCGGGGCGCTGGCTAGTTAGTTTTCTGTCTACCAGCCGTTGCGCAGCAGGGTGTAAACGATGCCAGAATCAGCCAGCATT   <  SRR4302533.1310873/94‑1 (MQ=60)

                                                                                             CGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAGGGTGTAAACGATGCCAGAATCAGCCAGCATTT  >  SRR4302533.2622311/1‑94 (MQ=60)

                                                                                             CGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAGGGTGTAAACGATGCCAGAATCAGCCAGCATTT  >  SRR4302533.2080747/1‑94 (MQ=60)

GCTTCGCTAATCACGCGTGCCGCAGCTGCCGCATAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGTGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAGGGTGTAAACGATGCCAGAATCAGCCAGCATTT  >  NC_000913/4431049‑4431235

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`