Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1624	250.6	12620048	98.0%	12367647	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,458,282	A→G	G651G (GGT→GGC)	nrdD ←	anaerobic ribonucleoside‑triphosphate reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,458,282	0	A	G	99.6%	802.7 / ‑4.8	251	G651G (GGT→GGC)	nrdD	anaerobic ribonucleoside‑triphosphate reductase
Reads supporting (aligned to +/- strand): ref base A (1/0); new base G (114/136); total (115/136)
Fisher's exact test for biased strand distribution p-value = 4.58e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.68e-01

Only 100 of 262 total aligned reads displayed.

CCGCACACGCGGCGAGTTACCGACACACGGGAGGCGTCATGGTTACCACATTTCGGGCAAGTGAAGCCTTTGCTGGTGCACTCGAACTCACCGGTAAAGCCACACTCGTAGCACTCATCAATCGGTGTATTGGTGCCGTAATACGGTACATGCTGATAGCTGTAATCCCAGACATCTTC  >  NC_000913/4458193‑4458371

 cGCACACGCGGCGAGTTACCGACACACGGGAGGCGTCATGGTTACCACATTTCGGGCAAGTGAAGCCTTTGCTGGTGCACTCGAACTCGCCgg                                                                                       <  1:4360705/93‑1 (MQ=255)

  gCACACGCGGCGAGTTACCGACACACGGGAGGCGTCATGGTTACCACATTTCGGGCAAGTGAAGCCTTTGCTGGTGCACTCGAACTCGCCGGt                                                                                      <  1:3943515/93‑1 (MQ=255)

   cacaCGCGGCGAGTTACCGACACACGGGAGGCGTCATGGTTACCACATTTCGGGCAAGTGAAGCCTTTGCTGGTGCACTCGAACTCGCCGGTa                                                                                     >  1:4912287/1‑93 (MQ=255)

       cgcgGCGAGTTACCGACACACGGGAGGCGTCATGGTTACCACATTTCGGGCAAGTGAAGCCTTTGCTGGTGCACTCGAACTCGCCGGTAAAGc                                                                                 <  2:344745/93‑1 (MQ=255)

       cgcgGCGAGTTACCGACACACGGGAGGCGTCATGGTTACCACATTTCGGGCAAGTGAAGCCTTTGCTGGTGCACTCGAACTCGCCGGTAAAGc                                                                                 <  2:3046083/93‑1 (MQ=255)

        gcggcgAGTTACCGACACACGGGAGGCGTCATGGTTACCACATTTCAGGCAAGTGAAGCCTTTGCTGGTGCACTCGAACTCGCCGGTAAAGcc                                                                                >  1:1256002/1‑93 (MQ=255)

         cggcgAGTTACCGACACACGGGAGGCGTCATGGTTACCACATTTCGGGCAAGTGAAGCCTTTGCTGGTGCACTCGAACTCGCCGGTAAAGCca                                                                               >  2:3653122/1‑93 (MQ=255)

           gcgAGTTACCGACACACGGGAGGCGTCATGGTTACCACATTTCGGGCAAGTGAAGCCTTTGCTGGTGCACTCGAACTCGCTGGTAAAGCcaca                                                                             <  1:5926077/93‑1 (MQ=255)

            cgAGTTACCGACACACGGGAGGCGTCATGGTTACCACATTTCGGGCAAGTGAAGCCTTTGCTGGTGCACTCGAACTCGCCGGTAAAGCCacac                                                                            <  1:5256526/93‑1 (MQ=255)

               gTTACCGACACACGGGAGGCGTCATGGTTACCACATTTCGGGCAAGTGAAGCCTTTGCTGGTGCACTCGAACTCGCCGGTAAAGCCACACTCg                                                                         <  1:4349698/93‑1 (MQ=255)

                 tACCGACACACGGGAGGCGTCATGGTTACCACATTTCGGGCAAGTGAAGCCTTTGCTGGTGCACTCGAACTCGCCGGTAAAGCCACACTCGTa                                                                       >  2:6227538/1‑93 (MQ=255)

                 tACCGACACACGGGAGGCGTCATGGTTACCACATTTCGGGCAAGTGAAGCCTTTGATGGTGCACTCGAACTCGCCGGTAAAGCCACACTCGTa                                                                       <  1:1725229/93‑1 (MQ=255)

                  aCCGACACACGGGAGGCGTCATGGTTACCACATTTCGGGCAAGTGAAGCCTTTGCTGGTGCACTCGAACTCGCCGGTAAAGCCACACTCGTAg                                                                      <  2:5249015/93‑1 (MQ=255)

                  aCCGACACACGGGAGGCGTCATGGTTACCACATTTCGGGCAAGTGAAGCCTTTGCTGGTGCACTCGAACTCGCCGGTAAAGCCACACTCGTAg                                                                      <  2:4886178/93‑1 (MQ=255)

                  aCCGACACACGGGAGGCGTCATGGTTACCACATTTCGGGCAAGTGAAGCCTTTGCTGGTGCACTCGAACTCGCCGGTAAAGCCACACTCGTAg                                                                      <  2:2878110/93‑1 (MQ=255)

                   ccGACACACGGGAGGCGTCATGGTTACCACATTTCGGGCAAGTGAAGCCTTTGCTGGTGCACTCGAACTCGCCGGTAAAGCCACACTCGTAGc                                                                     <  2:2475276/93‑1 (MQ=255)

                    cGACACACGGGAGGCGTCATGGTTACCACATTTCGGGCAAGTGAAGCCTTTGCTGGTGCACTCGAACTCGCCGGTAAAGCCACACTCGTAGCa                                                                    >  2:3185205/1‑93 (MQ=255)

                     gACACACGGGAGGCGTCATGGTTACCACATTTCGGGCAAGTGAAGCCTTTGCTGGTGCACTCGAACTCGCCGGTAAAGCCACACTCGTAGCAc                                                                   >  2:4786492/1‑93 (MQ=255)

                      acacacGGGAGGCGTCATGGTTACCACATTTCGGGCAAGTGAAGCCTTTGCTGGTGCACTCGAACTCGCCGGTAAAGCCACACTCGTAGCACt                                                                  >  2:3347001/1‑93 (MQ=255)

                       cacacGGGAGGCGTCATGGTTACCACATTTCGGGCAAGTGAAGCCTTTGCTGGTGCACTCGAACTCGCCGGTAAAGCCACACTCGTAGCACtc                                                                 >  2:4145960/1‑93 (MQ=255)

                        acacGGGAGGCGTCATGGTTACCACATTTCGGGCAAGTGAAGCCTTTGCTGGTGCACTCGAACTCGCCGGTAAAGCCACACTCGTAGCACtca                                                                <  1:989699/93‑1 (MQ=255)

                         cacGGGAGGCGTCATGGTTACCACATTTCGGGCAAGTGAAGCCTTTGCTGGTGCACTCGAACTCGCCGGTAAAGCCACACTCGTAGCACtcat                                                               >  1:5447595/1‑93 (MQ=255)

                         cacGGGAGGCGTCATGGTTACCACATTTCGGGCAAGCGAAGCCTTTGCTCGTGCACTCGAACTCGCCGGTAAAGCCACACTCCTAGCACtcat                                                               >  1:109148/1‑93 (MQ=255)

                          acGGGAGGCGTCATGGTTACCACATTTCGGGCAAGTGAAGCCTTTGCTGGTGCACTCGAACTCGCCGGTAAAGCCACACTCGTAGCACtcatc                                                              <  1:5750131/93‑1 (MQ=255)

                           cGGGAGGCGTCATGGTTACCACATTTCGGGCAAGTGAAGCCTTTGCTGGTGCACTCGAACTCGCCGGTAAAGCCACACTCGTAGCACtcatca                                                             <  1:1350563/93‑1 (MQ=255)

                            gggAGGCGTCATGGTTACCACATTTCGGGCAAGTGAAGCCTTTGCTGGTGCACTCGAACTCGCCGGTAAAGCCACACTCGTAGCACTCATCaa                                                            <  2:64489/93‑1 (MQ=255)

                             ggAGGCGTCATGGTTACCACATTTCGGGCAAGTGAAGCCTTTGCTGGTGCACTCGAACTCGCCGGTAAAGCCACACTCGTAGCACTCATCAAt                                                           >  1:317280/1‑93 (MQ=255)

                                ggCGTCATGGTTACCACATTTCGGGCAAGTGAAGCCTTTGCTGGTGCACTCGAACTCGCCGGTAAAGCCACACTCGTAGCACTCATCAATCgg                                                        <  1:1596157/93‑1 (MQ=255)

                                 gCGTCATGGTTACCACATTTCGGGCAAGTGAAGCCTTTGCTGGTGCACTCGAACTCGCCGGTAAAGCCACACTCGTAGCACTCATCAATCGgt                                                       <  2:3239711/93‑1 (MQ=255)

                                   gTCATGGTTACCACATTTCGGGCAAGTGAAGCCTTTGCTGGTGCACTCGAACTCGCCGGTAAAGCCACACTCGTAGCACTCATCAATCGgtgt                                                     >  2:2217160/1‑93 (MQ=255)

                                   gTCATGGTTACCACATTTCGGGCAAGTGAAGCCTTTGCTGGTGCACTCGAACTCGCCGGTAAAGCCACACTCGTAGCACTCATCAATCGgtgt                                                     >  1:1123407/1‑93 (MQ=255)

                                    tCATGGTTACCACATTTCGGGCAAGTGAAGCCTTTGCTGGTGCACTCGAACTCGCCGGTAAAGCCACACTCGTAGCACTCATCAATCGGTGTa                                                    >  2:1608648/1‑93 (MQ=255)

                                    tCATGGTTACCACATTTCGGGCAAGTGAAGCCTTTGCTGGTGCACTCGAACTCGCCGGTAAAGCCACACTCGTAGCACTCATCAATCGGTGTa                                                    >  1:5594515/1‑93 (MQ=255)

                                    tCATGGTTACCACATTTCGGGCAAGTGAAGCCTTTGCTGGTGCACTCGAACTCGCCGGTAAAGCCACACTCGTAGCACTCATCAATCGGTGTa                                                    >  1:500885/1‑93 (MQ=255)

                                     cATGGTTACCACATTTCGGGCAAGTGAAGCCTTTGCTGGTGCACTCGAACTCGCCGGTAAAGCCACACTCGTAGCACTCATCAATCGGTGTAt                                                   <  1:890632/93‑1 (MQ=255)

                                      aTGGTTACCACATTTCGGGCAAGTGAAGCCTTTGCTGGTGCACTCGAACTCGCCGGTAAAGCCACACTCGTAGCACTCATCAATCGGTGTAtt                                                  >  1:4133864/1‑93 (MQ=255)

                                       tGGTTACCACATTTCGGGCAAGTGAAGCCTTTGCTGGTGCACTCGAACTCGCCGGTAAAGCCACACTCGTAGCACTCATCAATCGGTGTATTg                                                 <  1:6123696/93‑1 (MQ=255)

                                         gTTACCACATTTCGGGCAAGTGAAGCCTTTGCTGGTGCACTCGAACTCGCCGGTAAAGCCACACTCGTAGCACTCATCAATCGGTGTATTGGt                                               <  1:821300/93‑1 (MQ=255)

                                         gTTACCACATTTCGGGCAAGTGAAGCCTTTGCTGGTGCACTCGAACTCGCCGGTAAAGCCACACTCGTAGCACTCATCAATCGGTGTATTGGt                                               <  1:1203867/93‑1 (MQ=255)

                                           tACCACATTTCGGGCAAGTGAAGCCTTTGCTGGTGCACTCGAACTCGCCGGTAAAGCCACACTCGTAGCACTCATCAATCGGTGTATTGGTGc                                             <  2:5074950/93‑1 (MQ=255)

                                           tACCACATTTCGGGCAAGTGAAGCCTTTGCTGGTGCACTCGAACTCGCCGGTAAAGCCACACTCGTAGCACTCATCAATCGGTGTATTGGTGc                                             <  2:2107287/93‑1 (MQ=255)

                                            aCCACATTTCGGGCAAGTGAAGCCTTTGCTGGTGCACTCGAACTCGCCGGTAAAGCCACACTCGTAGCACTCATCAATCGGTGTATTGGTGcc                                            <  2:858939/93‑1 (MQ=255)

                                            aCCACATTTCGGGCAAGTGAAGCCTTTGCTGGTGCACTCGAACTCGCCGGTAAAGCCACACTCGTAGCACTCATCAATCGGTGTATTGGTGcc                                            <  2:1870022/93‑1 (MQ=255)

                                              cacaTTTTGGGCAAGTGAAGCCTTTGCTGGTGCACTCGAACTCGCCGGTAAAGCCACACTCGTAGCACTCATCAATCGGTGTATTGGTGCCGt                                          >  1:3442308/1‑93 (MQ=255)

                                               ccaTTTCGGGCAAGTGAAGCCTTTGCTGGTGCACTCGAACTCGCCGGTAAAGCCACACTCGTAGCACTCATCAATCGGTGTATTGGTGCCGTa                                         <  2:496611/92‑1 (MQ=255)

                                                caTTTCGGGCAAGTGAAGCCTTTGCTGGTGCACTCGAACTCGCCGGTAAAGCCACACTCGTAGCACTCATCAATCGGTGTATTGGTGCCGTaa                                        <  1:2481680/93‑1 (MQ=255)

                                                 aTTTCGGGCAAGTGAAGCCTTTGCTGGTGCACTCGAACTCGCCGGTAAAGCCACACTCGTAGCACTCATCAATCGGTGTATTGGTGCCGTAAt                                       >  1:4604491/1‑93 (MQ=255)

                                                 aTTTCGGGCAAGTGAAGCCTTTGCTGGTGCACTCGAACTCGCCGGTAAAGCCACACTCGTAGCACTCATCAATCGGTGTATTGGTGCCGTAAt                                       <  1:2311150/93‑1 (MQ=255)

                                                  tttCGGGCAAGTGAAGCCTTTGCTGGTGCACTCGAACTCGCCGGTAAAGCCACACTCGTAGCACTCATCAATCGGTGTATTGGTGCCGTAATa                                      >  1:5188177/1‑93 (MQ=255)

                                                  tttCGGGCAAGTGAAGCCTTTGCTGGTGCACTCGAACTCGCCGGTAAAGCCACACTCGTAGCACTCATCAATCGGTGTATTGGTGCCGTAATa                                      <  1:5316687/93‑1 (MQ=255)

                                                    tCGGGCAAGTGAAGCCTTTGCTGGTGCACTCGAACTCGCCGGTAAAGCCACACTCGTAGCACTCATCAATCGGTGTATTGGTGCCGTAATACg                                    >  1:2181674/1‑93 (MQ=255)

                                                     cGGGCAAGGGAAGCCTTTGCTGGTGCCCTCGAACTCGCCGGTAAAGCCACACTCGTAGCACTCATCAATCGGTGTATTGGTGCCGTAATACgg                                   <  2:1223291/93‑1 (MQ=255)

                                                      gggCAAGTGAAGCCTTTGCTGGTGCACTCGAACTCGCCGGTAAAGCCACACTCGTAGCACTCATCAATCGGTGTATTGGTGCCGTAATACGGt                                  >  1:712643/1‑93 (MQ=255)

                                                      gggCAAGTGAAGCCTTTGCTGGTGCACTCGAACTCGCCGGTAAAGCCACACTCGTAGCACTCATCAATCGGTGTATTGGTGCCGTAATACGGt                                  <  2:3388140/93‑1 (MQ=255)

                                                         cAAGTGAAGCCTTTGCTGGTGCACTCGAACTCGCCGGTAAAGCCACACTCGTAGCACTCATCAATCGGTGTATTGGTGCCGTAATACGGTACa                               <  2:2665439/93‑1 (MQ=255)

                                                         cAAGTGAAGCCTTTGCTGGTGCACTCGAACTCGCCGGTAAAGCCACACTCGTAGCACTCATCAATCGGTGTATTGGTGCCGTAATACGGTACa                               <  1:3047410/93‑1 (MQ=255)

                                                         cAAGTGAAGCCTTTGCTGGTGCACTCGAACTCGCCGGTAAAGCCACACTCGTAGCACTCATCAATCGGTGTATTGGTGCCGTAATACGGTACa                               <  2:5348372/93‑1 (MQ=255)

                                                          aaGTGAAGCCTTTGCTGGTGCACTCGAACTCGCCGGTAAAGCCACACTCGTAGCACTCATCAATCGGTGTATTGGTGCCGTAATACGGTACAt                              <  2:5857472/93‑1 (MQ=255)

                                                          aaGTGAAGCCTTTGCTGGTGCACTCGAACTCGCCGGTAAAGCCACACTCGTAGCACTCATCAATCGGTGTATTGGTGCCGTAATACGGTACAt                              >  1:3016365/1‑93 (MQ=255)

                                                           aGTGAAGCCTTTGCTGGTGCACTCGAACTCGCCGGTAAAGCCACACTCGTAGCACTCATCAATCGGTGTATTGGTGCCGTAATACGGTACATg                             >  2:1534025/1‑93 (MQ=255)

                                                            gTGAAGCCTTTGCTGGTGCCCTCGACCTCGCCGGTAAAGCCACACTCGTAGCACTCATCAATCGGTGTATTGGTGCCGTAATACGGTACATGc                            <  2:5676663/93‑1 (MQ=255)

                                                            gTGAAGCCTTTGCTGGTGCACTCGAACTCGCCGGTAAAGCCACACTCGTAGCACTCATCAATCGGTGTATTGGTGCCGTAATACGGTACATGc                            >  1:2546956/1‑93 (MQ=255)

                                                            gTGAAGCCTTTGCTGGTGCACTCGAACTCGCCGGTAAAGCCACACTCGTAGCACCCATCAATCGGTGTATTGGTGCCGTAATACGGTACATGc                            <  1:4051521/93‑1 (MQ=255)

                                                             tGAAGCCTTTGCTGGTGCACTCGAACTCGCCGGTAAAGCCACACTCGTAGCACTCATCAATCGGTGTATTGGTGCCGTAATACGGTACATGCt                           <  1:3319661/93‑1 (MQ=255)

                                                             tGAAGCCTTTGCTGGTGCACTCGAACTCGCCGGTAAAGCCACACTCGTAGCACTCATCAATCGGTGTATTGGTGCCGTAATACGGTACATGCt                           <  2:1061566/93‑1 (MQ=255)

                                                             tGAAGCCTTTGCTGGTGCACTCGAACTCGCCGGTAAAGCCACACTCGTAGCACTCATCAATCGGTGTATTGGTGCCGTAATACGGTACATGCt                           <  2:713662/93‑1 (MQ=255)

                                                              gAAGCCTTTGCTGGTGCACTCGAACTCGCCGGTAAAGCCACACTCGTAGCACTCATCAATCGGTGTATTGGTGCCGTAATACGGTACATGCTg                          <  1:3554446/93‑1 (MQ=255)

                                                                aGCCTTTGCTGGTGCACTCGAACTCGCCGGTAAAGCCACACTCGTAGCACTCATCAATCGGTGTATTGGTGCCGTAATACGGTACATGCTGAt                        <  2:4211213/93‑1 (MQ=255)

                                                                aGCCTTTGCTGGTGCACTCGAACTCGCCGGTAAAGCCACACTCGTAGCACTCATCAATCGGTGTATTGGTGCCGTAATACGGTACATGCTGAt                        >  1:1950951/1‑93 (MQ=255)

                                                                 gCCTTTGCTGGTGCACTCGAACTCGCCGGTAAAGCCACACTCGTAGCACTCATCAATCGGTGTATTGGTGCCGTAATACGGTACATGCTGATa                       <  1:4007585/93‑1 (MQ=255)

                                                                  ccTTTGCTGGTGCACTCGAACTCGCCGGTAAAGCCACACTCGTAGCACTCATCAATCGGTGTATTGGTGCCGTAATACGGTACATGCTGATAg                      >  2:3960840/1‑93 (MQ=255)

                                                                  ccTTTGCTGGTGCACTCGAACTCGCCGGTAAAGCCACACTCGTAGCACTCATCAATCGGTGTATTGGTGCCGTAATACGGTACATGCTGATAg                      >  1:2630943/1‑93 (MQ=255)

                                                                  ccTTTGCTGGTGCACTCGAACTCGCCGGTAAAGCCACACTCGTAGCACTCATCAATCGGTGTATTGGTGCCGTAATACGGTACATGCTGATAg                      <  2:2355946/93‑1 (MQ=255)

                                                                   cTTTGCTGGTGCACTCGAACTCGCCGGTAAAGCCACACTCGTAGCACTCATCAATCGGTGTATTGGTGCCGTAATACGGTACATGCTGATAGc                     >  1:3887086/1‑93 (MQ=255)

                                                                    tttGCTGGTGCACTCGAACTCGCCGGTAAAGCCACACTCGTAGCACTCATCAATCGGTGTATTGGTGCCGTAATACGGTACATGCTGATAGCt                    >  1:4762660/1‑93 (MQ=255)

                                                                    tttGCTGGTGCACTCGAACTCGCCGGTAAAGCCACACTCGTAGCACTCATCAATCGGTGTATTGGTGCCGTAATACGGTACATGCTGATAGCt                    <  2:1419939/93‑1 (MQ=255)

                                                                    tttGCTGGTGCACTCGAACTCGCCGGTAAAGCCACACTCGTAGCACTCATCAATCGGTGTATTGGTGCCGTAATACGGTACATGCTGATAGCt                    <  2:5989564/93‑1 (MQ=255)

                                                                     ttGCTGGTGCACTCGAACTCGCCGGTAAAGCCACACTCGTAGCACTCATCAATCGGTGTATTGGTGCCGTAATACGGTACATGCTGATAGCTg                   <  1:2825770/93‑1 (MQ=255)

                                                                        cTGGTGCACTCGAACTCGCCGGTAAAGCCACACTCGTAGCACTCATCAATCGGTGTATTGGTGCCGTAATACGGTACATGCTGATAGCTGTaa                <  2:2424884/93‑1 (MQ=255)

                                                                        cTGGTGCACTCGAACTCGCCGGTAAAGCCACACTCGTAGCACTCATCAATCGGTGTATTGGTGCCGTAATACGGTACATGCTGATAGCTGTaa                <  2:2125177/93‑1 (MQ=255)

                                                                         tGGTGCACTCGAACTCGCCGGTAAAGCCACACTCGTAGCACTCATCAATCGGTGTATTGGTGCCGTAATACGGTACATGCTGATAGCTGTAAt               >  1:2929035/1‑93 (MQ=255)

                                                                         tGGTGCACTCGAACTCGCCGGTAAAGCCACACTCGTAGCACTCATCAATCGGTGTATTGGTGCCGTAATACGGTACATGCTGATAGCTGTAAt               >  2:5494223/1‑93 (MQ=255)

                                                                         tGGTGCACTCGAACTCGCCGGTAAAGCCACACTCGTAGCACTCATCAATCGGTGTATTAGTGCCGTAATACGGTACATGCTGATAGCTGTAAt               >  1:5771381/1‑93 (MQ=255)

                                                                          ggTGCACTCGAACTCGCCGGTAAAGCCACACTCGTAGCACTCATCAATCGGTGTATTGGTGCCGTAATACGGTACATGCTGATAGCTGTAATc              <  2:2547161/93‑1 (MQ=255)

                                                                          ggTGCACTCGAACTCGCCGGTAAAGCCACACTCGTAGCACTCATCAATCGGTGTATTGGTGCCGTAATACGGTACATGCTGATAGCTGTAATc              >  2:1708435/1‑93 (MQ=255)

                                                                           gTGCACTCGAACTCGCCGGTAAAGCCACACTCGTAGCACTCATCAATCGGTGTATTGGTGCCGTAATACGGTACATGCTGATAGCTGTAATcc             >  1:4699982/1‑93 (MQ=255)

                                                                            tGCACTCGAACTCGCCGGTAAAGCCACACTCGTAGCACTCATCAATCGGTGTATTGGTGCCGTAATACGGTACATGCTGATAGCTGTAATccc            <  1:3225582/93‑1 (MQ=255)

                                                                             gCACTCGAACTCGCCGGTAAAGCCACACTCGTAGCACTCATCAATCGGTGTATTGGTGCCGTAATACGGTACATGCTGATAGCTGTAATCCCa           <  2:370683/93‑1 (MQ=255)

                                                                             gCACTCGAACTCGCCGGTAAAGCCACACTCGTAGCACTCATCAATCGGTGTATTGGTGCCGTAATACGGTACATGCTGATAGCTGTAATCCCa           <  1:1628722/93‑1 (MQ=255)

                                                                             gCACTCGAACTCGCCGGTAAAGCCACACTCGTAGCACTCATCAATCGGTGTATTGGTGCCGTAATACGGTACATGCTGATAGCTGTAATCCCa           <  2:6076331/93‑1 (MQ=255)

                                                                                cTCGAACTCGCCGGTAAAGCCACACTCGTAGCACTCATCAATCGGTGTATTGGTGCCGTAATACGGTACATGCTGATAGCTGTAATCCCAGAc        >  2:3325784/1‑93 (MQ=255)

                                                                                cTCGAACTCACCGGTAAAGCCACACTCGTAGCACTCATCAATCGGTGTATTGGTGCCGTAATACGGTACATGCTGATAGCTGTAATCCCAGAc        >  1:466251/1‑93 (MQ=255)

                                                                                 tCGAACTCGCCGGTAAAGCCACACTCGTAGCACTCATCAATCGGTGTATTGGTGCCGTAATACGGTACATGCTGATAGCTGTAATCCCAGACa       <  2:6098415/93‑1 (MQ=255)

                                                                                   gAACTCGCCGGTAAAGCCACACTCGTAGCACTCATCAATCGGTGTATTGGTGCCGTAATACGGTACATGCTGATAGCTGTAATCCCAGACATc     <  1:1700204/93‑1 (MQ=255)

                                                                                   gAACTCGCCGGTAAAGCCACACTCGTAGCACTCATCAATCGGTGTATTGGTGCCGTAATACGGTACATGCTGATAGCTGTAATCCCAGACATc     <  2:4363072/93‑1 (MQ=255)

                                                                                    aaCTCGCCGGTAAAGCCACACTCGTAGCACTCATCAATCGGTGTATTGGTGCCGTAATACGGTACATGCTGATAGCTGTAATCCCAGACATCt    <  2:3611461/93‑1 (MQ=255)

                                                                                     aCTCGCCGGTAAAGCCACACTCGTAGCACTCATCAATCGGTGTATTGGTGCCGTAATACGGTACATGCTGATAGCTGTAATCCCAGACATCtt   <  1:334693/93‑1 (MQ=255)

                                                                                     aCTCGCCGGTAAAGCCACACTCGTAGCACTCATCAATCGGTGTATTGGTGCCGTAATACGGTACATGCTGATAGCTGTAATCCCAGACATCtt   >  2:2920637/1‑93 (MQ=255)

                                                                                      cTCGCCGGTAAAGCCACACTCGTAGCACTCATCAATCGGTGTATTGGTGCCGTAATACGGTACATGCTGATAGCTGTAATCCCAGACATCTTc  >  1:5965054/1‑93 (MQ=255)

                                                                                      cTCGCCGGTAAAGCCACACTCGTAGCACTCATCAATCGGTGTATTGGTGCCGTAATACGGTACATGCTGATAGCTGTAATCCCAGACATCTTc  >  1:2692396/1‑93 (MQ=255)

CCGCACACGCGGCGAGTTACCGACACACGGGAGGCGTCATGGTTACCACATTTCGGGCAAGTGAAGCCTTTGCTGGTGCACTCGAACTCACCGGTAAAGCCACACTCGTAGCACTCATCAATCGGTGTATTGGTGCCGTAATACGGTACATGCTGATAGCTGTAATCCCAGACATCTTC  >  NC_000913/4458193‑4458371

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

TATCCGCACACGCGGCGAGTTACCGACACACGGGAGGCGTCATGGTTACCACATTTCGGGCAAGTGAAGCCTTTGCTGGTGCACTCGAACTCACCGGTAAAGCCACACTCGTAGCACTCATCAATCGGTGTATTGGTGCCGTAATACGGTACATGCTGATAGCTGTAATCCCAGACATCTTCCAGC  >  NC_000913/4458190‑4458375

TATCCGCACACGCGGCGAGTTACCGACACACGGGAGGCGTCATGGTTACCACATTTCGGGCAAGTGAAGCCTTTGCTGGTGCACTCGAACTCGC                                                                                              >  SRR4302533.2340188/1‑94 (MQ=60)

 ATCCGCACACGCGGCGAGTTACCGACACACGGGAGGCGTCATGGTTACCACATTTCGGGCAAGTGAAGCCTTTGCTGGTGCACTCGAACTCGCC                                                                                             <  SRR4302533.4810198/94‑1 (MQ=60)

 ATCCGCACACGCGGCGAGTTACCGACACACGGGAGGCGTCATGGTTACCACATTTCGGGCAAGTGAAGCCTTTGCTGGTGCACTCGAACTCGCC                                                                                             <  SRR4302533.500375/94‑1 (MQ=60)

  TCCGCACACGCGGCGAGTTACCGACACACGGGAGGCGTCATGGTTACCACATTTCGGGCAAGTGAAGCCTTTGCTGGTGCACTCGAACTCGCCG                                                                                            >  SRR4302533.3024617/1‑94 (MQ=60)

   CCGCACACGCGGCGAGTTACCGACACACGGGAGGCGTCATGGTTACCACATTTCGGGCAAGTGAAGCCTTTGCTGGTGCACTCGAACTCGCCGG                                                                                           >  SRR4302533.3475263/1‑94 (MQ=60)

    CGCACACGCGGCGAGTTACCGACACACGGGAGGCGTCATGGTTACCACATTTCGGGCAAGTGAAGCCTTTGCTGGTGCACTCGAACTCGCCGGT                                                                                          >  SRR4302533.1895047/1‑94 (MQ=60)

    CGCACACGCGGCGAGTTACCGACACACGGGAGGCGTCATGGTTACCACATTTCGGGCAAGTGAAGCCTTTGCTGGTGCACTCGAACTCGCCGGT                                                                                          <  SRR4302533.4387028/94‑1 (MQ=60)

     GCACACGCGGCGAGTTACCGACACACGGGAGGCGTCATGGTTACCACATTTCGGGCAAGTGAAGCCTTTGCTGGTGCACTCGAACTCGCCGGTA                                                                                         >  SRR4302533.4942045/1‑94 (MQ=60)

     GCACACGCGGCGAGTTACCGACACACGGGAGGCGTCATGGTTACCACATTTCGGGCAAGTGAAGCCTTTGCTGGTGCACTCGAACTCGCCGGTA                                                                                         <  SRR4302533.3967571/94‑1 (MQ=60)

     GCACACGCGGCGAGTTACCGACACACGGGAGGCGTCATGGTTACCACATTTCGGGCAAGTGAAGCCTTTGCTGGTGCACTCGAACTCGCCGGTA                                                                                         >  SRR4302533.5195729/1‑94 (MQ=60)

     GCACACGCGGCGAGTTACCGACACACGGGAGGCGTCATGGTTACCACATTTCGGGCAAGTGAAGCCTTTGCTGGTGCACTCGAACTCGCCGGTA                                                                                         >  SRR4302533.4942046/1‑94 (MQ=60)

      CACACGCGGCGAGTTACCGACACACGGGAGGCGTCATGGTTACCACATTTCGGGCAAGTGAAGCCTTTGCTGGTGCACTCGAACTCGCCGGTAA                                                                                        <  SRR4302533.1272345/94‑1 (MQ=60)

      CACACGCGGCGAGTTACCGACACACGGGAGGCGTCATGGTTACCACATTTCGGGCAAGTGAAGCCTTTGCTGGTGCACTCGAACTCGCCGGTAA                                                                                        >  SRR4302533.6100285/1‑94 (MQ=60)

      CACACGCGGCGAGTTACCGACACACGGGAGGCGTCATGGTTACCACATTTCGGGCAAGTGAAGCCTTTGCTGGTGCACTCGAACTCGCCGGTAA                                                                                        <  SRR4302533.5093068/94‑1 (MQ=60)

        CACGCGGCGAGTTACCGACACACGGGAGGCGTCATGGTTACCACATTTCGGGCAAGTGAAGCCTTTGCTGGTGCACTCGAACTCGCCGGTAAAG                                                                                      <  SRR4302533.2771307/94‑1 (MQ=60)

        CACGCGGCGAGTTACCGACACACGGGAGGCGTCATGGTTACCACATTTCGGGCAAGTGAAGCCTTTGCTGGTGCACTCGAACTCGCCGGTAAAG                                                                                      >  SRR4302533.161162/1‑94 (MQ=60)

         CCGCGGCGAGTTACCGACACACGGGAGGCGTCATGGTTACCACATTGCGGGCAAGTGAAGCCTTTGCTGGTGCACTCGAACTCGCCGGTAAAGC                                                                                     <  SRR4302533.2953090/94‑1 (MQ=60)

         ACGCGGCGAGTTACCGACACACGGGAGGCGTCATGGTTACCACATTTCGGGCAAGTGAAGCCTTTGCTGGTGCACTCGAACTCGCCGGTAAAGC                                                                                     <  SRR4302533.5790465/94‑1 (MQ=60)

          CGCGGCGAGTTACCGACACACGGGAGGCGTCATGGTTACCACATTTCGGGCAAGTGAAGCCTTTGCTGGTGCACTCGAACTCGCCGGTAAAGCC                                                                                    <  SRR4302533.2262001/94‑1 (MQ=60)

          CGCGGCGAGTTACCGACACACGGGAGGCGTCATGGTTACCACATTTCGGGCAAGTGAAGCCTTTGCTGGTGCACTCGAACTCGCCGGTAAAGCC                                                                                    <  SRR4302533.3345872/94‑1 (MQ=60)

          CGCGGCGAGTTACCGACACACGGGAGGCGTCATGGTTACCACATTTCAGGCAAGTGAAGCCTTTGCTGGTGCACTCGAACTCGCCGGTAAAGCC                                                                                    >  SRR4302533.1263511/1‑94 (MQ=60)

           GCGGCGAGTTACCGACACACGGGAGGCGTCATGGTTACCACATTTCGGGCAAGTGAAGCCTTTGCTGGTGCACTCGAACTCGCCGGTAAAGCCA                                                                                   <  SRR4302533.3064633/94‑1 (MQ=60)

             GGCGAGTTACCGACACACGGGAGGCGTCATGGTTACCACATTTCGGGCAAGTGAAGCCTTTGCTGGTGCACTCGAACTCGCCGGTAAAGCCACA                                                                                 >  SRR4302533.5966639/1‑94 (MQ=60)

             GGCGAGTTACCGACACACGGGAGGCGTCATGGTTACCACATTTCGGGCAAGTGAAGCCTTTGCTGGTGCACTCGAACTCGCCGGTAAAGCCACA                                                                                 <  SRR4302533.3891801/94‑1 (MQ=60)

              GCGAGTTACCGACACACGGGAGGCGTCATGGTTACCACATTTCGGGCAAGTGAAGCCTTTGCTGGTGCACTCGAACTCGCTGGTAAAGCCACAC                                                                                <  SRR4302533.5961900/94‑1 (MQ=60)

              GCGAGTTACCGACACACGGGAGGCGTCATGGTTACCACATTTCGGGCAAGTGAAGCCTTTGCTGGTGCACTCGAACTCGCCGGTAAAGCCACAC                                                                                <  SRR4302533.1719095/94‑1 (MQ=60)

               CGAGTTACCGACACACGGGAGGCGTCATGGTTACCACATTTCGGGCAAGTGAAGCCTTTGCTGGTGCACTCGAACTCGCCGGTAAAGCCACACT                                                                               <  SRR4302533.2684776/94‑1 (MQ=60)

               CGAGTTACCGACACACGGGAGGCGTCATGGTTACCACATTTCGGGCAAGTGAAGCCTTTGCTGGTGCACTCGAACTCGCCGGTAAAGCCACACT                                                                               <  SRR4302533.5288096/94‑1 (MQ=60)

                  GTTACCGACACACGGGAGGCGTCATGGTTACCACATTTCGGGCAAGTGAAGCCTTTGCTGGTGCACTCGAACTCGCCGGTAAAGCCACACTCGT                                                                            <  SRR4302533.4375920/94‑1 (MQ=60)

                    TACCGACACACGGGAGGCGTCATGGTTACCACATTTCGGGCAAGTGAAGCCTTTGCTGGTGCACTCGAACTCGCCGGTAAAGCCACACTCGTAG                                                                          <  SRR4302533.4723707/94‑1 (MQ=60)

                    TACCGACACACGGGAGGCGTCATGGTTACCACATTTCGGGCAAGTGAAGCCTTTGCTGGTGCACTCGAACTCGCCGGTAAAGCCACACTCGTAG                                                                          >  SRR4302533.733384/1‑94 (MQ=60)

                    TACCGACACACGGGAGGCGTCATGGTTACCACATTTCGGGCAAGTGAAGCCTTTGATGGTGCACTCGAACTCGCCGGTAAAGCCACACTCGTAG                                                                          <  SRR4302533.1735699/94‑1 (MQ=60)

                      CCGACACACGGGAGGCGTCATGGTTACCACATTTCGGGCAAGTGAAGCCTTTGCTGGTGCACTCGAACTCGCCGGTAAAGCCACACTCGTAGCA                                                                        <  SRR4302533.3234682/94‑1 (MQ=60)

                       CGACACACGGGAGGCGTCATGGTTACCACATTTCGGGCAAGTGAAGCCTTTGCTGGTGCACTCGAACTCGCCGGTAAAGCCACACTCGTAGCAC                                                                       <  SRR4302533.5815324/94‑1 (MQ=60)

                       CGACACACGGGAGGCGTCATGGTTACCACATTTCGGGCAAGTGAAGCCTTTGCTGGTGCACTCGAACTCGCCGGTAAAGCCACACTCGTAGCAC                                                                       >  SRR4302533.5688564/1‑94 (MQ=60)

                         ACACACGGGAGGCGTCATGGTTACCACATTTCGGGCAAGTGAAGCCTTTGCTGGTGCACTCGAACTCGCCGGTAAAGCCACACTCGTAGCACTC                                                                     <  SRR4302533.4029959/94‑1 (MQ=60)

                          CACACGGGAGGCGTCATGGTTACCACATTTCGGGCAAGTGAAGCCTTTGCTGGTGCACTCGAACTCGCCGGTAAAGCCACACTCGTAGCACTCA                                                                    <  SRR4302533.1708833/94‑1 (MQ=60)

                          CACACGGGAGGCGTCATGGTTACCACATTTCGGGCAAGTGAAGCCTTTGCTGGTGCACTCGAACTCGCCGGTAAAGCCACACTCGTAGCACTCA                                                                    >  SRR4302533.2404140/1‑94 (MQ=60)

                           ACACGGGAGGCGTCATGGTTACCACATTTCGGGCAAGTGAAGCCTTTGCTGGTGCACTCGAACTCGCCGGTAAAGCCACACTCGTAGCACTCAT                                                                   >  SRR4302533.5480413/1‑94 (MQ=60)

                           ACACGGGAGGCGTCATGGTTACCACATTTCGGGCAAGTGAAGCCTTTGCTGGTGCACTCGAACTCGCCGGTAAAGCCACACTCGTAGCACTCAT                                                                   <  SRR4302533.995490/94‑1 (MQ=60)

                           ACACGGGAGGCGTCATGGTTACCACATTTCGGGCAAGCGAAGCCTTTGCTCGTGCACTCGAACTCGCCGGTAAAGCCACACTCCTAGCACTCAT                                                                   >  SRR4302533.109843/1‑94 (MQ=60)

                             ACGGGAGGCGTCATGGTTACCACATTTCGGGCAAGTGAAGCCTTTGCTGGTGCACTCGAACTCGCCGGTAAAGCCACACTCGTAGCACTCATCA                                                                 <  SRR4302533.3398201/94‑1 (MQ=60)

                             ACGGGAGGCGTCATGGTTACCACATTTCGGGCAAGTGAAGCCTTTGCTGGTGCACTCGAACTCGCCGGTAAAGCCACACTCGTAGCACTCATCA                                                                 <  SRR4302533.3252553/94‑1 (MQ=60)

                             ACGGGAGGCGTCATGGTTACCACATTTCGGGCAAGTGAAGCCTTTGCTGGTGCACTCGAACTCGCCGGTAAAGCCACACTCGTAGCACTCATCA                                                                 <  SRR4302533.5784820/94‑1 (MQ=60)

                             ACGGGAGGCGTCATGGTTACCACATTTCGGGCAAGTGAAGCCTTTGCTGGTGCACTCGAACTCGCCGGTAAAGCCACACTCGTAGCACTCATCA                                                                 <  SRR4302533.3220218/94‑1 (MQ=60)

                              CGGGAGGCGTCATGGTTACCACATTTCGGGCAAGTGAAGCCTTTGCTGGTGCACTCGAACTCGCCGGTAAAGCCACACTCGTAGCACTCATCAA                                                                >  SRR4302533.2531770/1‑94 (MQ=60)

                              CGGGAGGCGTCATGGTTACCACATTTCGGGCAAGTGAAGCCTTTGCTGGTGCACTCGAACTCGCCGGTAAAGCCACACTCGTAGCACTCATCAA                                                                >  SRR4302533.726171/1‑94 (MQ=60)

                              CGGGAGGCGTCATGGTTACCACATTTCGGGCAAGTGAAGCCTTTGCTGGTGCACTCGAACTCGCCGGTAAAGCCACACTCGTAGCACTCATCAA                                                                <  SRR4302533.1358685/94‑1 (MQ=60)

                               GGGAGGCGTCATGGTTACCACATTTCGGGCAAGTGAAGCCTTTGCTGGTGCACTCGAACTCGCCGGTAAAGCCACACTCGTAGCACTCATCAAT                                                               >  SRR4302533.319222/1‑94 (MQ=60)

                                 GAGGCGTCATGGTTACCACATTTCGGGCAAGTGAAGCCTTTGCTGGTGCACTCGAACTCGCCGGTAAAGCCACACTCGTAGCACTCATCAATCG                                                             <  SRR4302533.5892229/94‑1 (MQ=60)

                                  AGGCGTCATGGTTACCACATTTCGGGCAAGTGAAGCCTTTGCTGGTGCACTCGAACTCGCCGGTAAAGCCACACTCGTAGCACTCATCAATCGG                                                            >  SRR4302533.13320/1‑94 (MQ=60)

                                   GGCGTCATGGTTACCACATTTCGGGCAAGTGAAGCCTTTGCTGGTGCACTCGAACTCGCCGGTAAAGCCACACTCGTAGCACTCATCAATCGGT                                                           <  SRR4302533.1605817/94‑1 (MQ=60)

                                    GCGTCATGGTTACCACATTTCGGGCAAGTGAAGCCTTTGCTGGTGCACTCGAACTCGCCGGTAAAGCCACACTCGTAGCACTCATCAATCGGTG                                                          <  SRR4302533.90289/94‑1 (MQ=60)

                                     CGTCATGGTTACCACATTTCGGGCAAGTGAAGCCTTTGCTGGTGCACTCGAACTCGCCGGTAAAGCCACACTCGTAGCACTCATCAATCGGTGT                                                         >  SRR4302533.432472/1‑94 (MQ=60)

                                     CGTCATGGTTACCACATTTCGGGCAAGTGAAGCCTTTGCTGGTGCACTCGAACTCGCCGGTAAAGCCACACTCGTAGCACTCATCAATCGGTGT                                                         >  SRR4302533.1130072/1‑94 (MQ=60)

                                      GTCATGGTTACCACATTTCGGGCAAGTGAAGCCTTTGCTGGTGCACTCGAACTCGCCGGTAAAGCCACACTCGTAGCACTCATCAATCGGTGTA                                                        >  SRR4302533.5715104/1‑94 (MQ=60)

                                      GTCATGGTTACCACATTTCGGGCAAGTGAAGCCTTTGCTGGTGCACTCGAACTCGCCGGTAAAGCCACACTCGTAGCACTCATCAATCGGTGTA                                                        >  SRR4302533.5628313/1‑94 (MQ=60)

                                      GTCATGGTTACCACATTTCGGGCAAGTGAAGCCTTTGCTGGTGCACTCGAACTCGCCGGTAAAGCCACACTCGTAGCACTCATCAATCGGTGTA                                                        >  SRR4302533.503866/1‑94 (MQ=60)

                                        CATGGTTACCACATTTCGGGCAAGTGAAGCCTTTGCTGGTGCACTCGAACTCGCCGGTAAAGCCACACTCGTAGCACTCATCAATCGGTGTATT                                                      >  SRR4302533.4158954/1‑94 (MQ=60)

                                        CATGGTTACCACATTTCGGGCAAGTGAAGCCTTTGCTGGTGCACTCGAACTCGCCGGTAAAGCCACACTCGTAGCACTCATCAATCGGTGTATT                                                      <  SRR4302533.895865/94‑1 (MQ=60)

                                        CATGGTTACCACATTTCGGGCAAGTGAAGCCTTTGCTGGTGCACTCGAACTCGCCGGTAAAGCCACACTCGTAGCACTCATCAATCGGTGTATT                                                      >  SRR4302533.287697/1‑94 (MQ=60)

                                        CATGGTTACCACATTTCGGGCAAGTGAAGCCTTTGCTGGTGCACTCGAACTCGCCGGTAAAGCCACACTCGTAGCACTCATCAATCGGTGTATT                                                      <  SRR4302533.4627845/94‑1 (MQ=60)

                                        CATGGTTACCACATTTCGGGCAAGTGAAGCCTTTGCTGGTGCACTCGAACTCGCCGGTAAAGCCACACTCGTAGCACTCATCAATCGGTGTATT                                                      >  SRR4302533.2338190/1‑94 (MQ=60)

                                        CATGGTTACCACATTTCGGGCAAGTGAAGCCTTTGCTGGTGCACTCGAACTCGCCGGCAAAGCCACACTCGTAGCACTCATCAATCGGTGTATT                                                      >  SRR4302533.4289027/1‑94 (MQ=60)

                                          TGGTTACCACATTTCGGGCAAGTGAAGCCTTTGCTGGTGCACTCGAACTCGCCGGTAAAGCCACACTCGTAGCACTCATCAATCGGTGTATTGG                                                    <  SRR4302533.6160611/94‑1 (MQ=60)

                                            GTTACCACATTTCGGGCAAGTGAAGCCTTTGCTGGTGCACTCGAACTCGCCGGTAAAGCCACACTCGTAGCACTCATCAATCGGTGTATTGGTG                                                  <  SRR4302533.1211100/94‑1 (MQ=60)

                                            GTTACCACATTTCGGGCAAGTGAAGCCTTTGCTGGTGCACTCGAACTCGCCGGTAAAGCCACACTCGTAGCACTCATCAATCGGTGTATTGGTG                                                  >  SRR4302533.3938407/1‑94 (MQ=60)

                                            GTTACCACATTTCGGGCAAGTGAAGCCTTTGCTGGTGCACTCGAACTCGCCGGTAAAGCCACACTCGTAGCACTCATCAATCGGTGTATTGGTG                                                  <  SRR4302533.826118/94‑1 (MQ=60)

                                             TTACCACATTTCGGGCAAGTGAAGCCTTTGCTGGTGCACTCGAACTCGCCGGTAAAGCCACACTCGTAGCACTCATCAATCGGTGTATTGGTGC                                                 >  SRR4302533.904956/1‑94 (MQ=60)

                                               ACCACATTTCGGGCAAGTGAAGCCTTTGCTGGTGCACTCGAACTCGCCGGTAAAGCCACACTCGTAGCACTCATCAATCGGTGTATTGGTGCCG                                               >  SRR4302533.1144617/1‑94 (MQ=60)

                                               ACCACATTTCGGGCAAGTGAAGCCTTTGCTGGTGCACTCGAACTCGCCGGTAAAGCCACACTCGTAGCACTCATCAATCGGTGTATTGGTGCCG                                               >  SRR4302533.1706863/1‑94 (MQ=60)

                                                CCACATTTTGGGCAAGTGAAGCCTTTGCTGGTGCACTCGAACTCGCCGGTAAAGCCACACTCGTAGCACTCATCAATCGGTGTATTGGTGCCGT                                              >  SRR4302533.3463571/1‑94 (MQ=60)

                                                CCACATTTCGGGCAAGTGAAGCCTTTGCTGGTGCACTCGAACTCGCCGGTAAAGCCACACTCGTAGCACTCATCAATCGGTGTATTGGTGCCGT                                              >  SRR4302533.4377993/1‑94 (MQ=60)

                                                  ACATTTCGGGCAAGTGAAGCCTTTGCTGGTGCACTCGAACTCGCCGGTAAAGCCACACTCGTAGCACTCATCAATCGGTGTATTGGTGCCGTAA                                            >  SRR4302533.2534479/1‑94 (MQ=60)

                                                   CATTTCGGGCAAGTGAAGCCTTTGCTGGTGCACTCGAACTCGCCGGTAAAGCCACACTCGTAGCACTCATCAATCGGTGTATTGGTGCCGTAAT                                           >  SRR4302533.4632386/1‑94 (MQ=60)

                                                   CATTTCGGGCAAGTGAAGCCTTTGCTGGTGCACTCGAACTCGCCGGTAAAGCCACACTCGTAGCACTCATCAATCGGTGTATTGGTGCCGTAAT                                           <  SRR4302533.3104471/94‑1 (MQ=60)

                                                   CATTTCGGGCAAGTGAAGCCTTTGCTGGTGCACTCGAACTCGCCGGTAAAGCCACACTCGTAGCACTCATCAATCGGTGTATTGGTGCCGTAAT                                           <  SRR4302533.2496985/94‑1 (MQ=60)

                                                   CATTTCGGGCAAGTGAAGCCTTTGCTGGTGCACTCGAACTCGCCGGTAAAGCCACACTCGTAGCACTCATCAATCGGTGTATTGGTGCCGTAAT                                           <  SRR4302533.5991038/94‑1 (MQ=60)

                                                    ATTTCGGGCAAGTGAAGCCTTTGCTGGTGCACTCGAACTCGCCGGTAAAGCCACACTCGTAGCACTCATCAATCGGTGTATTGGTGCCGTAATA                                          >  SRR4302533.5219488/1‑94 (MQ=60)

                                                    ATTTCGGGCAAGTGAAGCCTTTGCTGGTGCACTCGAACTCGCCGGTAAAGCCACACTCGTAGCACTCATCAATCGGTGTATTGGTGCCGTAATA                                          <  SRR4302533.2325468/94‑1 (MQ=60)

                                                    ATTTCGGGCAAGTGAAGCCTTTGCTGGTGCACTCGAACTCGCCCGTAAAGCCACACTCGTAGCACTCATCAATCGGTGTATTGGTGCCGTAATA                                          >  SRR4302533.4456236/1‑94 (MQ=60)

                                                     TTTCGGGCAAGTGAAGCCTTTGCTGGTGCACTCGAACTCGCCGGTAAAGCCACACTCGTAGCACTCATCAATCGGTGTATTGGTGCCGTAATAC                                         <  SRR4302533.5348548/94‑1 (MQ=60)

                                                     TTTCGGGCAAGTGAAGCCTTTGCTGGTGCACTCGAACTCGCCGGTAAAGCCACACTCGTAGCACTCATCAATCGGTGTATTGGTGCCGTAATAC                                         <  SRR4302533.4146173/94‑1 (MQ=60)

                                                      TTCGGGCAAGTGAAGCCTTTGCTGGTGCACTCGAACTCGCCGGTAAAGCCACACTCGTAGCACTCATCAATCGGTGTATTGGTGCCGTAATACG                                        >  SRR4302533.2195124/1‑94 (MQ=60)

                                                      TTCGGGCAAGTGAAGCCTTTGCTGGTGCACTCGAACTCGCCGGTAAAGCCACACTCGTAGCACTCATCAATCGGTGTATTGGTGCCGTAATACG                                        <  SRR4302533.4108540/94‑1 (MQ=60)

                                                      TTCGGGCAAGTGAAGCCTTTGCTGGTGCACTCGAACTCGCCGGTAAAGCCACACTCGTAGCACTCATCAATCGGTGTATTGGTGCCGTAATACG                                        <  SRR4302533.2588593/94‑1 (MQ=60)

                                                      TTCGGGCAAGTGAAGCCTTGGCTGGTGCACTCGAACTCGCCGGTAAAGCCACACTCGTAGCACTCATCAATCGGTGTATTGGTGCCGTAATACG                                        <  SRR4302533.2899739/94‑1 (MQ=60)

                                                        CGGGCAAGTGAAGCCTTTGCTGGTGCACTCGAACTCGCCGGTAAAGCCACACTCGTAGCACTCATCAATCGGTGTATTGGTGCCGTAATACGGT                                      <  SRR4302533.3749655/94‑1 (MQ=60)

                                                        CGGGCAAGTGAAGCCTTTGCTGGTGCACTCGAACTCGCCGGTAAAGCCACACTCGTAGCACTCATCAATCGGTGTATTGGTGCCGTAATACGGT                                      <  SRR4302533.1176027/94‑1 (MQ=60)

                                                        CGGGCAAGTGAAGCCTTTGCTGGTGCACTCGAACTCGCCGGTAAAGCCACACTCGTAGCACTCATCAATCGGTGTATTGGTGCCGTAATACGGT                                      >  SRR4302533.716849/1‑94 (MQ=60)

                                                           GCAAGTGAAGCCTTTGCTGGTGCACTCGAACTCGCCGGTAAAGCCACACTCGTAGCACTCATCAATCGGTGTATTGGTGCCGTAATACGGTACA                                   <  SRR4302533.865585/94‑1 (MQ=60)

                                                            CAAGTGAAGCCTTTGCTGGTGCACTCGAACTCGCCGGTAAAGCCACACTCGTAGCACTCATCAATCGGTGTATTGGTGCCGTAATACGGTACAT                                  >  SRR4302533.3491941/1‑94 (MQ=60)

                                                            CAAGTGAAGCCTTTGCTGGTGCACTCGAACTCGCCGGTAAAGCCACACTCGTAGCACTCATCAATCGGTGTATTGGTGCCGTAATACGGTACAT                                  <  SRR4302533.468491/94‑1 (MQ=60)

                                                            CAAGTGAAGCCTTTGCTGGTGCACTCGAACTCGCCGGTAAAGCCACACTCGTAGCACTCATCAATCGGTGTATTGGTGCCGTAATACGGTACAT                                  <  SRR4302533.3066067/94‑1 (MQ=60)

                                                            CAAGTGAAGCCTTTGCTGGTGCACTCGAACTCGCCGGTAAAGCCACACTCGTAGCACTCATCAATCGGTGTATTGGTGCCGTAATACGGTACAT                                  >  SRR4302533.3034814/1‑94 (MQ=60)

                                                             AAGTGAAGCCTTTGCTGGTGCACTCGAACTCGCCGGTAAAGCCACACTCGTAGCACTCATCAATCGGTGTATTGGTGCCGTAATACGGTACATG                                 <  SRR4302533.4009111/94‑1 (MQ=60)

                                                              AGTGAAGCCTTTGCTGGTGCACTCGAACTCGCCGGTAAAGCCACACTCGTAGCACTCATCAATCGGTGTATTGGTGCCGTAATACGGTACATGC                                >  SRR4302533.2562628/1‑94 (MQ=60)

                                                               GTGAAGCCTTTGCTGGTGCACTCGAACTCGCCGGTAAAGCCACACTCGTAGCACCCATCAATCGGTGTATTGGTGCCGTAATACGGTACATGCT                               <  SRR4302533.4076198/94‑1 (MQ=60)

                                                                TGAAGCCTTTGCTGGTGCACTCGAACTCGCCGGTAAAGCCACACTCGTAGCACTCATCAATCGGTGTATTGGTGCCGTAATACGGTACATGCTG                              <  SRR4302533.3340165/94‑1 (MQ=60)

                                                                 GAAGCCTTTGCTGGTGCACTCGAACTCGCCGGTAAAGCCACACTCGTAGCACTCATCAATCGGTGTATTGGTGCCGTAATACGGTACATGCTGA                             >  SRR4302533.4054116/1‑94 (MQ=60)

                                                                 GAAGCCTTTGCTGGTGCACTCGAACTCGCCGGTAAAGCCACACTCGTAGCACTCATCAATCGGTGTATTGGTGCCGTAATACGGTACATGCTGA                             <  SRR4302533.3576347/94‑1 (MQ=60)

                                                                 GAAGCCTTTGCTGGTGCACTCGAACTCGCCGGTAAAGCCACACTCGTAGCACTCATCAATCGGTGTATTGGTGCAGTAATACGGTACATGCTGA                             >  SRR4302533.3123226/1‑94 (MQ=60)

                                                                  AAGCCTTTGCTGGTGCACTCGAACTCGCCGGTAAAGCCACACTCGTAGCACTCATCAATCGGTGTATTGGTGCCGTAATACGGTACATGCTGAT                            >  SRR4302533.1962840/1‑94 (MQ=60)

                                                                   AGCCTTTGCTGGTGCACTCGAACTCGCCGGTAAAGCCACACTCGTAGCACTCATCAATCGGTGTATTGGTGCCGTAATACGGTACATGCTGATA                           >  SRR4302533.1108570/1‑94 (MQ=60)

                                                                   AGCCTTTGCTGGTGCACTCGAACTCGCCGGTAAAGCCACACTCGTAGCACTCATCAATCGGTGTATTGGTGCCGTAATACGGTACATGCTGATA                           <  SRR4302533.5306405/94‑1 (MQ=60)

                                                                   AGCCTTTGCTGGTGCACTCGAACTCGCCGGTAAAGCCACACTCGTAGCACTCATCAATCGGTGTATTGGTGCCGTAATACGGTACATGCTGATA                           <  SRR4302533.5516873/94‑1 (MQ=60)

                                                                   AGCCTTTGCTGGTGCACTCGAACTCGCCGGTAAAGCCACACTCGTAGCACTCATCAATCGGTGTATTGGTGCCGTAATACGGTACATGCTGATA                           <  SRR4302533.6313011/94‑1 (MQ=60)

                                                                   AGCCTTTGCTGGTGCACTCGAACTCGCCGGTAAAGCCACACTCGTAGCACTCATCAATCGGTGTATTGGTGCCGTAATACGGTACATGCTGATA                           >  SRR4302533.1591958/1‑94 (MQ=60)

                                                                   AGCCTTTGCTGGTGCACTCGAACTCGCCGGTAAAGCCACACTCGTAGCACTCATCAATCGGTGTATTGGTGCCGTAATACGGTACATGCTGATA                           <  SRR4302533.2156474/94‑1 (MQ=60)

                                                                    GCCTTTGCTGGTGCACTCGAACTCGCCGGTAAAGCCACACTCGTAGCACTCATCAATCGGTGTATTGGTGCCGTAATACGGTACATGCTGATAG                          >  SRR4302533.2647113/1‑94 (MQ=60)

                                                                    GCCTTTGCTGGTGCACTCGAACTCGCCGGTAAAGCCACACTCGTAGCACTCATCAATCGGTGTATTGGTGCCGTAATACGGTACATGCTGATAG                          <  SRR4302533.5277155/94‑1 (MQ=60)

                                                                    GCCTTTGCTGGTGCACTCGAACTCGCCGGTAAAGCCACACTCGTAGCACTCATCAATCGGTGTATTGGTGCCGTAATACGGTACATGCTGATAG                          <  SRR4302533.4031984/94‑1 (MQ=60)

                                                                    GCCTTTGCTGGTGCACTCGAACTCGCCGGTAAAGCCACACTCGTAGCACTCATCAATCGGTGTATTGGTGCCGTAATACGGTACATGCTGATAG                          <  SRR4302533.3349897/94‑1 (MQ=60)

                                                                     CCTTTGCTGGTGCACTCGAACTCGCCGGTAAAGCCACACTCGTAGCACTCATCAATCGGTGTATTGGTGCCGTAATACGGTACATGCTGATAGC                         <  SRR4302533.1249721/94‑1 (MQ=60)

                                                                     CCTTTGCTGGTGCACTCGAACTCGCCGGTAAAGCCACACTCGTAGCACTCATCAATCGGTGTATTGGTGCCGTAATACGGTACATGCTGATAGC                         >  SRR4302533.3910801/1‑94 (MQ=60)

                                                                      CTTTGCTGGTGCACTCGAACTCGCCGGTAAAGCCACACTCGTAGCACTCATCAATCGGTGTATTGGTGCCGTAATACGGTACATGCTGATAGCT                        >  SRR4302533.4791545/1‑94 (MQ=60)

                                                                      CTTTGCTGGTGCACTCGAACTCGCCGGTAAAGCCACACTCGTAGCACTCATCAATCGGTGTATTGGTGCCGTAATACGGTACATGCTGATAGCT                        >  SRR4302533.5336279/1‑94 (MQ=60)

                                                                       TTTGCTGGTGCACTCGAACTCGCCGGTAAAGCCACACTCGTAGCACTCATCAATCGGTGTATTGGTGCCGTAATACGGTACATGCTGATAGCTG                       <  SRR4302533.4791037/94‑1 (MQ=60)

                                                                        TTGCTGGTGCACTCGAACTCGCCGGTAAAGCCACACTCGTAGCACTCATCAATCGGTGTATTGGTGCCGTAATACGGTACATGCTGATAGCTGT                      <  SRR4302533.2843158/94‑1 (MQ=60)

                                                                          GCTGGTGCACTCGAACTCGCCGGTAAAGCCACACTCGTAGCACTCATCAATCGGTGTATTGGTGCCGTAATACGGTACATGCTGATAGCTGTAA                    <  SRR4302533.4640215/94‑1 (MQ=60)

                                                                           CTGGTGCACTCGAACTCGCCGGTAAAGCCACACTCGTAGCACTCATCAATCGGTGTATTGGTGCCGTAATACGGTACATGCTGATAGCTGTAAT                   >  SRR4302533.4989755/1‑94 (MQ=60)

                                                                           CTGGTGCACTCGAACTCGCCGGTAAAGCCACACTCGTAGCACTCATCAATCGGTGTATTGGTGCCGTAATACGGTACATGCTGATAGCTGTAAT                   >  SRR4302533.2946953/1‑94 (MQ=60)

                                                                           CTGGTGCACTCGAACTCGCCGGTAAAGCCACACTCGTAGCACTCATCAATCGGTGTATTAGTGCCGTAATACGGTACATGCTGATAGCTGTAAT                   >  SRR4302533.5806230/1‑94 (MQ=60)

                                                                            TGGTGCACTCGAACTCGCCGGTAAAGCCACACTCGTAGCACTCATCAATCGGTGTATTGGTGCCGTAATACGGTACATGCTGATAGCTGTAATC                  <  SRR4302533.694784/94‑1 (MQ=60)

                                                                             GGTGCACTCGAACTCGCCGGTAAAGCCACACTCGTAGCACTCATCAATCGGTGTATTGGTGCCGTAATACGGTACATGCTGATAGCTGTAATCC                 >  SRR4302533.4728480/1‑94 (MQ=60)

                                                                               TGCACTCGAACTCGCCGGTAAAGCCACACTCGTAGCACTCATCAATCGGTGTATTGGTGCCGTAATACGGTACATGCTGATAGCTGTAATCCCA               <  SRR4302533.3245469/94‑1 (MQ=60)

                                                                                GCACTCGAACTCGCCGGTAAAGCCACACTCGTAGCACTCATCAATCGGTGTATTGGTGCCGTAATACGGTACATGCTGATAGCTGTAATCCCAG              <  SRR4302533.1638587/94‑1 (MQ=60)

                                                                                 CACTCGAACTCGCCGGTAAAGCCACACTCGTAGCACTCATCAATCGGTGTATTGGTGCCGTAATACGGTACATGCTGATAGCTGTAATCCCAGA             <  SRR4302533.4790421/94‑1 (MQ=60)

                                                                                  ACTCGAACTCGCCGGTAAAGCCACACTCGTAGCACTCATCAATCGGTGTATTGGTGCCGTAATACGGTACATGCTGATAGCTGTAATCCCAGAC            >  SRR4302533.2673842/1‑94 (MQ=60)

                                                                                  ACTCGAACTCACCGGTAAAGCCACACTCGTAGCACTCATCAATCGGTGTATTGGTGCCGTAATACGGTACATGCTGATAGCTGTAATCCCAGAC            >  SRR4302533.469037/1‑94 (MQ=60)

                                                                                  ACTCGAACACGCCGGTAAAGCCACACTCGTAGCACTCATCAATCGGTGTATTGGTGCCGTAATACGGTACATGCTGATAGCTGTAATCCCAGAC            <  SRR4302533.4815479/94‑1 (MQ=60)

                                                                                   CTCGAACTCGCCGGTAAAGCCACACTCGTAGCACTCATCAATCGGTGTATTGGTGCCGTAATACGGTACATGCTGATAGCTGTAATCCCAGACA           >  SRR4302533.2068164/1‑94 (MQ=60)

                                                                                     CGAACTCGCCGGTAAAGCCACACTCGTAGCACTCATCAATCGGTGTATTGGTGCCGTAATACGGTACATGCTGATAGCTGTAATCCCAGACATC         >  SRR4302533.5388237/1‑94 (MQ=60)

                                                                                      GAACTCGCCGGTAAAGCCACACTCGTAGCACTCATCAATCGGTGTATTGGTGCCGTAATACGGTACATGCTGATAGCTGTAATCCCAGACATCT        <  SRR4302533.1710508/94‑1 (MQ=60)

                                                                                       AACTCGCCGGTAAAGCCACACTCGTAGCACTCATCAATCGGTGTATTGGTGCCGTAATACGGTACATGCTGATAGCTGTAATCCCAGACATCTT       >  SRR4302533.5358082/1‑94 (MQ=60)

                                                                                        ACTCGCCGGTAAAGCCACACTCGTAGCACTCATCAATCGGTGTATTGGTGCCGTAATACGGTACATGCTGATAGCTGTAATCCCAGACATCTTC      >  SRR4302533.6001151/1‑94 (MQ=60)

                                                                                        ACTCGCCGGTAAAGCCACACTCGTAGCACTCATCAATCGGTGTATTGGTGCCGTAATACGGTACATGCTGATAGCTGTAATCCCAGACATCTTC      >  SRR4302533.2708897/1‑94 (MQ=60)

                                                                                        ACTCGCCGGTAAAGCCACACTCGTAGCACTCATCAATCGGTGTATTGGTGCCGTAATACGGTACATGCTGATAGCTGTAATCCCAGACATCTTC      <  SRR4302533.336739/94‑1 (MQ=60)

                                                                                         CTCGCCGGTAAAGCCACACTCGTAGCACTCATCAATCGGTGTATTGGTGCCGTAATACGGTACATGCTGATAGCTGTAATCCCAGACATCTTCC     >  SRR4302533.3885455/1‑94 (MQ=60)

                                                                                         CTCGCCGGTAAAGCCACACTCGTAGCACTCATCAATCGGTGTATTGGTGCCGTAATACGGTACATGCTGATAGCTGTAATCCCAGACATCTTCC     <  SRR4302533.1421153/94‑1 (MQ=60)

                                                                                         CTCGCCGGTAAAGCCACACTCGTAGCACTCATCAATCGGTGTATTGGTGCCGTAATACGGTACATGCTGATAGCTGTAATCCCAGACATCTTCC     <  SRR4302533.1934301/94‑1 (MQ=60)

                                                                                         CTCGCCGGTAAAGCCACACTCGTAGCACTCATCAATCGGTGTATTGGTGCCGTAATACGGTACATGCTGATAGCTGTAATCCCAGACATCTTCC     >  SRR4302533.5350421/1‑94 (MQ=60)

                                                                                         CTCGCCGGTAAAGCCACACTCGTAGCACTCATCAATCGGTGTATTGGTGCCGTAATACGGTACATGCTGATAGCTGTAATCCCAGACATCTTCC     >  SRR4302533.5884595/1‑94 (MQ=60)

                                                                                         CTCGCCGGTAAAGCCACACTCGTAGCACTCATCAATCGGTGTATTGGTGCCGTAATACGGTACATGCTGATAGCTGTAATCCCAGACATCTTCC     >  SRR4302533.6010339/1‑94 (MQ=60)

                                                                                          TCGCCGGTAAAGCCACACTCGTAGCACTCATCAATCGGTGTATTGGTGCCGTAATACGGTACATGCTGATAGCTGTAATCCCAGACATCTTCCA    <  SRR4302533.1929361/94‑1 (MQ=60)

                                                                                          TCGCCGGTAAAGCCACACTCGTAGCACTCATCAATCGGTGTATTGGTGCCGTAATACGGTACATGCTGATAGCTGTAATCCCAGACATCTTCCA    <  SRR4302533.3852952/94‑1 (MQ=60)

                                                                                          TCGCCGGTAAAGCCACACTCGTAGCACTCATCAATCGGTGTATTGGTGCCGTAATACGGTACATGCTGATAGCTGTAATCCCAGACATCTTCCA    >  SRR4302533.1509395/1‑94 (MQ=60)

                                                                                           CGCCGGTAAAGCCACACTCGTAGCACTCATCAATCGGTGTATTGGTGCCGTAATACGGTACATGCTGATAGCTGTAATCCCAGACATCTTCCAG   <  SRR4302533.2198914/94‑1 (MQ=60)

                                                                                           CGCCGGTAAAGCCACACTCGTAGCACTCATCAATCGGTGTATTGGTGCCGTAATACGGTACATGCTGATAGCTGTAATCCCAGACATCTTCCAG   >  SRR4302533.5605747/1‑94 (MQ=60)

                                                                                           CGCCGGTAAAGCCACACTCGTAGCACTCATCAATCGGTGTATTGGTGCCGTAATACGGTACATGCTGATAGCTGTAATCCCAGACATCTTCCAG   <  SRR4302533.1429659/94‑1 (MQ=60)

                                                                                           CGCCGGTAAAGCCACACTCGTAGCACTCATCAATCGGTGTATTGGTGCCGTAATACGGTACATGCTGATAGCTGTAATCCCAGACATCTTCCAG   <  SRR4302533.2503774/94‑1 (MQ=60)

                                                                                            GCCGGTAAAGCCACACTCGTAGCACTCATCAATCGGTGTATTGGTGCCGTAATACGGTACATGCTGATAGCTGTAATCCCAGACATCTTCCAGC  <  SRR4302533.6261076/94‑1 (MQ=60)

                                                                                            GCCGGTAAAGCCACACTCGTAGCACTCATCAATCGGTGTATTGGTGCCGTAATACGGTACACGCTGATAGCTGTAATCCCAGACATCTTCCAGC  >  SRR4302533.4783921/1‑94 (MQ=60)

TATCCGCACACGCGGCGAGTTACCGACACACGGGAGGCGTCATGGTTACCACATTTCGGGCAAGTGAAGCCTTTGCTGGTGCACTCGAACTCACCGGTAAAGCCACACTCGTAGCACTCATCAATCGGTGTATTGGTGCCGTAATACGGTACATGCTGATAGCTGTAATCCCAGACATCTTCCAGC  >  NC_000913/4458190‑4458375

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`