Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 647 270.1 21258240 98.4% 20918108 94.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,212,429 A→G D581G (GAC→GGC)  rpoD → RNA polymerase sigma(70)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,212,4290AG99.0% 1748.0 / 1.5 521D581G (GAC→GGC) rpoDRNA polymerase sigma(70)
Reads supporting (aligned to +/- strand):  ref base A (4/1);  new base G (257/257);  total (261/260)
Fisher's exact test for biased strand distribution p-value = 3.73e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.69e-01

Only 100 of 546 total aligned reads displayed.

CTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTGGGTAAACAGTTCGACGTTACCCGCGAACGTATCCGTCAGATCGAAGCGAAGGCGCTGCGCAAACTGCGTCACCCGAGCCGTTCTGAAGTGCTGCGTAGCTTCCT  >  NC_000913/3212338‑3212519
                                                                                           |                                                                                          
cTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTGGGTAAACAGTTCGGCg                                                                                          >  1:1970057/1‑94 (MQ=255)
 tGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTGGGTAAACAGTTCGGCGt                                                                                         <  2:5731782/94‑1 (MQ=255)
   aCCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTGGGTAAACAGTTCGGCGTTa                                                                                       <  2:3010400/94‑1 (MQ=255)
    ccGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTGGGTAAACAGTTCGGCGTTAc                                                                                      >  2:5056822/1‑94 (MQ=255)
     cgcgcgTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTGGGTAAACAGTTCGGCGTTAcc                                                                                     >  2:8270313/1‑94 (MQ=255)
     cgcgcgTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTGGGTAAACAGTTCGGCGTTAcc                                                                                     <  2:10195131/94‑1 (MQ=255)
      gcgcgTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTGGGTAAACAGTTCGGCGTTAccc                                                                                    <  2:1337695/94‑1 (MQ=255)
       cgcgTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTGGGTAAACAGTTCGGCGTTACCcg                                                                                   <  1:6072232/94‑1 (MQ=255)
         cgTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTGGGTAAACAGTTCGGCGTTACCcgcg                                                                                 <  1:1151775/94‑1 (MQ=255)
          gTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTGGGTAAACAGTTCGGCGTTACCCGCGa                                                                                >  2:6307986/1‑94 (MQ=255)
          gTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTGGGTAAACAGTTCGGCGTTACCCGCGa                                                                                <  1:4280865/94‑1 (MQ=255)
            gAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACTGACTACACGCTGGAAGAAGTGGGTAAACAGTTCGGCGTTACCCGCGAAc                                                                              >  2:3424322/1‑94 (MQ=255)
            gAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTGGGTAAACAGTTCGGCGTTACCCGCGAAc                                                                              >  1:8058985/1‑94 (MQ=255)
            gAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTGGGTAAACAGTTCGGCGTTACCCGCAAAc                                                                              >  1:4772360/1‑94 (MQ=255)
             aaGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTGGGTAAACAGTTCGGCGTTACCCGCGAACg                                                                             >  2:3820837/1‑94 (MQ=255)
              aGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTGGGTAAACAGTTCGGCGTTACCCGCGCACGt                                                                            >  1:3454017/1‑94 (MQ=255)
              aGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTGGGTAAACAGTTCGGCGTTACCCGCGAACGt                                                                            >  2:6042836/1‑94 (MQ=255)
                cAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTGGGTAAACAGTTCGGCGTTACCCGCGAACGTAt                                                                          <  2:650867/94‑1 (MQ=255)
                cAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTGGGTAAACAGTTCGGCGTTACCCGCGAACGTAt                                                                          <  1:5503552/94‑1 (MQ=255)
                 aaaaGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTGGGTAAACAGTTCGGCGTTACCCGCGAACGTATc                                                                         >  2:8707564/1‑94 (MQ=255)
                  aaaGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTGGGTAAACAGTTCGGCGTTACCCGCGAACGCATcc                                                                        >  1:7116162/1‑94 (MQ=255)
                  aaaGTTCTGCGTATGCGTGTCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTGGGTAAACAGTTCGGCGTTACCCGCGAACGTATcc                                                                        <  2:7037297/94‑1 (MQ=255)
                   aaGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTGGGTAAACAGTTCGGCGTTACCCGCGAACGTATCCg                                                                       >  1:4909425/1‑94 (MQ=255)
                   aaGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTGGGTAAACAGTTCGGCGTTACCCGCGAACGTATCCg                                                                       <  1:10341426/94‑1 (MQ=255)
                     gTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTGGGTAAACAGTTCGGCGTTACCCGCGAACGTATCCGTc                                                                     <  2:9313328/94‑1 (MQ=255)
                     gTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTGGGTAAACAGTTCGGCGTTACCCGCGAACGTATCCGTc                                                                     >  1:3661496/1‑94 (MQ=255)
                     attatGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTGGGTAAACAGTTCGGCGTTCCCCGCGAACGTATCCGTc                                                                     <  2:1093679/90‑1 (MQ=255)
                      ttCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTGGGTAAACAGTTCGGCGTTACCCGCGAACGTATCCGTCa                                                                    <  2:6736286/94‑1 (MQ=255)
                        cTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTGGGTAAACAGTTCGGCGTTACCCGCGAACGTATCCGTCAGa                                                                  >  1:9490196/1‑94 (MQ=255)
                          gCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTGGGTAAACAGTTCGGCGTTACCCGCGAACGTATCCGTCAGATc                                                                >  2:8313169/1‑94 (MQ=255)
                             tATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTGGGTAAACAGTTCGGCGTTACCCGCGAACGTATCCGTCAGATcgaa                                                             <  1:6405337/94‑1 (MQ=255)
                              aTGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTGGGTAAACAGTTCGGCGTTACCCGCGAACGTATCCGTCAGATcgaag                                                            >  1:8687513/1‑94 (MQ=255)
                              aTGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTGGGTAAACAGTTCGGCGTTACCCGCGAACGTATCCGTCAGATcgaag                                                            <  1:6784469/94‑1 (MQ=255)
                               tGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTGGGTAAACAGTTCGGCGTTACCCGCGAACGTATCCGTCAGATcgaagc                                                           >  1:5373043/1‑94 (MQ=255)
                               tGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTGGGTAAACAGTTCGGCGTTACCCGCGAACGTATCCGTCAGATcgaagc                                                           <  2:4410308/94‑1 (MQ=255)
                               tGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTGGGTAAACAGTTCGGCGTTACCCGCGAACGTATCCGTCAGATcgaagc                                                           <  1:4610213/94‑1 (MQ=255)
                                gCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTGGGTAAACAGTTCGGCGTTACCCGCGAACGTATCCGTCAGATcgaagca                                                          >  2:1968086/1‑93 (MQ=255)
                                 cGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTGGGTAAACAGTTCGGCGTTACCCGCGAACGTATCCGTCAGATcgaagcga                                                         >  2:5334823/1‑94 (MQ=255)
                                 cGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTGGGTAAACAGTTCGGCGTTACCCGCGAACGTATCCGTCAGATcgaagcga                                                         <  2:4534544/94‑1 (MQ=255)
                                 cGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTGGGTAAACAGTTCGGCGTTACCCGCGAACGTATCCGTCAGATcgaagcga                                                         <  2:2055020/94‑1 (MQ=255)
                                  gTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTGGGTAAACAGTTCGGCGTTACCCGCGAACGTATCCGTCAGATcgaagcgaa                                                        <  2:2366410/94‑1 (MQ=255)
                                     tCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTGGGTAAACAGTTCGGCGTTACCCGCGAACGTATCCGTCAGATCGAAGCGAAGgc                                                     <  2:2702317/94‑1 (MQ=255)
                                     tCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTGGGTAAACAGTTCGGCGTTACCCGCGAACGTATCCGTCAGATCGAAGCGAAGgc                                                     >  1:8239346/1‑94 (MQ=255)
                                      cGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTGGGTAAACAGTTCGGCGTTACCCGCGAACGTATCCGTCAGATCGAAGGGAAGgcg                                                    >  1:5726356/1‑94 (MQ=255)
                                      cGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTGGGTAAACAGTTCGGCGTTACCCGCGAACGTATCCGTCAGATCGAAGCGAAGgcg                                                    >  2:7418559/1‑94 (MQ=255)
                                      cGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTGGGTAAACAGTTCGGCGTTACCCGCGAACGTATCCGTCAGATCGAAGCGAAGgcg                                                    >  1:814622/1‑94 (MQ=255)
                                      cGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTGGGAAAAAAGTTCGGCGTTACCCGCGAACGTATCCGTCAGAGCGAAGCGAATgcg                                                    >  2:6907820/1‑94 (MQ=255)
                                       ggTATCGATATGAACACCGACTACACGCTGGAAGAAGTGGGTAAACAGTTCGGCGTTACCCGCGAACGTATCCGTCAGATCGAAGCGAAGgcgc                                                   <  1:5243490/94‑1 (MQ=255)
                                        gTATCGATATGAACACCGACTACACGCTGGAAGAAGTGGGTAAACAGTTCGGCGTTACCCGCGAACGTATCCGTCAGATCGAAGCGAAGGCGCt                                                  >  1:3337824/1‑94 (MQ=255)
                                         tATCGATATGAACACCGACTACACGCTGGAAGAAGTGGGTAAACAGTTCGGCGTTACCCGCGCACGTATCCGTCAGATCGAAGCGAAGGCGCTg                                                 >  1:4405300/1‑94 (MQ=255)
                                         tATCGATATGAACACCGACTACACGCTGGAAGAAGTGGGTAAACAGTTCGGCGTTACCCGCGAACGTATCCGTCAGATCGAATCGAAGGCGCTg                                                 <  1:1779299/94‑1 (MQ=255)
                                         tATCGATATGAACACCGACTACACGCTGGAAGAAGTGGGTAAACAGTTCGGCGTTACCCGCGAACGTATCCGTCAGATCGAAGCGAAGGCGCTg                                                 >  1:3157146/1‑94 (MQ=255)
                                          aTCGATATGAACACCGACTACACGCTGGAAGAAGTGGGTAAACAGTTCGGCGTTACCCGCGAACGTATCCGTCAGATCGAAGCGAAGGCGCTgc                                                <  1:1416737/94‑1 (MQ=255)
                                           tCGATATGAACACCGACTACACGCTGGAAGAAGTGGGTAAACAGTTCGGCGTTACCCGCGAACGTATCCGTCAGATCGAAGCGAAGGCGCTgcg                                               <  1:5930079/94‑1 (MQ=255)
                                             gATATGAACACCGACTACACGCTGGAAGAAGTGGGTAAACAGTTCGGCGTTACCCGCGAACGTATCCGTCAGATCGAAGCGAAGGCGCTGCGCa                                             >  2:9454535/1‑94 (MQ=255)
                                             gATATGAACACCGACTACACGCTGGAAGAAGTGGGTAAACAGTTCGGCGTTACCCGCGAACGTATCCGTCAGATCGAAGCGAAGGCGCTGCGCa                                             >  1:8615987/1‑94 (MQ=255)
                                             gATATGAACACCGACTACACGCTGGAAGAAGTGGGTAAACAGTTCGGCGTTACCCGCGAACGTATCCGTCAGATCGAAGCGAAGGCGCTGCGCa                                             >  2:5595108/1‑94 (MQ=255)
                                             gATATGAACACCGACTACACGCTGGAAGAAGTGGGTAAACAGTTCGGCGTTACCCGCGAACGTATCCGTCAGATCGAAGCGAAGGCGCTGCGCa                                             >  2:311399/1‑94 (MQ=255)
                                              atatGAACACCGACTACACGCTGGAAGAAGTGGGTAAACAGTTCGGCGTTACCCGCGAACGTATCCGTCAGATCGAAGCGAAGGCGCTGCGCaa                                            >  2:3897557/1‑94 (MQ=255)
                                               tatGAACACCGACTACACGCTGGAAGAAGTGGGTAAACAGTTCGGCGTTACCCGCGAACGTATCCGTCAGATCGAAGCGAAGGCGCTGCGCaaa                                           <  2:1808810/94‑1 (MQ=255)
                                               tatGAACACCGACTACACGCTGGAAGAAGTGGGTAAACAGTTCGGCGTTACCCGCGAACGTATCCGTCAGATCGAAGCGAAGGCGCTGCGCaaa                                           <  2:4266267/94‑1 (MQ=255)
                                                atGAACACCGACTACACGCTGGAAGAAGTGGGTAAACAGTTCGGCGTTACCCGCGAACGTATCCGTCAGATCGAAGCGAAGGCGCTGCGCAAAc                                          <  1:2200892/94‑1 (MQ=255)
                                                atGAACACCGACTACACGCTGGAAGAAGTGGGTAAACAGTTCGGCGTTACCCGCGAACGTATCCGTCAGATCGAAGCGAAGGCGCTGCGCAAAc                                          <  1:9972812/94‑1 (MQ=255)
                                                atGAACACCGACTACACGCTGGAAGAAGTGGGTAAACAGTTCGGCGTTACCCGCGAACGTATCCGTCAGATCGAAGCGAAGGCGCTGCGCAAAc                                          >  2:9638327/1‑94 (MQ=255)
                                                  gAACACCGACTACACGCTGGAAGAAGTGGGTAAACAGTTCGGCGTTACCCGCGAACGTATCCGTCAGATCGAAGCGAAGGCGCTGCGCAAACTg                                        <  1:6964067/94‑1 (MQ=255)
                                                   aaCACCGACTACACGCTGGAAGAAGTGGGTAAACAGTTCGGCGTTACCCGCGAACGTATCCGTCAGATCGAAGCGAAGGCGCTGCGCAAACTGc                                       >  2:9132144/1‑94 (MQ=255)
                                                     cacCGACTACACGCTGGAAGAAGTGGGTAAACAGTTCGGCGTTACCCGCGAACGTATCCGTCAGATCGAAGCGAAGGGCCTGCGCAAACTGCgc                                     >  1:9186685/1‑93 (MQ=255)
                                                     cacCGACTACACGCTGGAAGAAGTGGGTAAACAGTTCGGCGTTACCCGCGAACGTATCCGTCAGATCGAAGCGAAGGCGCTGCGCAAACTGCGt                                     >  1:10515682/1‑94 (MQ=255)
                                                       ccGACTACACGCTGGAAGAAGTGGGTAAACAGTTCGGCGTTACCCGCGAACGTATCCGTCAGATCGAAGCGAAGGCGCTGCGCAAACTGCGTCa                                   <  2:3588196/94‑1 (MQ=255)
                                                            tACACGCTGGAAGAAGTGGGTAAACAGTTCGGCGTTACCCGCGAACGTATCCGTCAGATCGAAGCGAAGGCGCTGCGCAAACTGCGTCACCCGa                              >  2:8524428/1‑94 (MQ=255)
                                                              cacGCTGGAAGAAGTGGGTAAACAGTTCGGCGTTACCCGCGAACGTATCCGTCAGATCGAAGCGAAGGCGCTGCGCAAACTGCGTCACCCGAGc                            >  1:6500874/1‑94 (MQ=255)
                                                               acGCTGGAAGAAGTGGGTAAACAGTTCGGCGTTACCCGCGAACGTATCCGTCAGATCGAAGCGAAGGCGCTGCGCAAACTGCGTCACCCGAGcc                           >  1:404706/1‑94 (MQ=255)
                                                                cGCTGGAAGAAGTGGGTAAACAGTTCGGCGTTACCCGCGAACGTATCCGTCAGATCGAAGCGAAGGCGCTGCGCAAACTGCGTCACCCGAGCCg                          <  1:7502414/94‑1 (MQ=255)
                                                                  cTGGAAGAAGTGGGTAAACAGTTCGGCGTTACCCGCGAACGTATCCGTCAGATCGAAGCGAAGGCGCTGCGCAAACTGCGTCACCCGAGCCGtt                        <  1:3862286/94‑1 (MQ=255)
                                                                    ggAAGAAGTGGGTAAACAGTTCGGCGTTACCCGCGAACGTATCCGTCAGATCGAAGCGAAGGCGCTGCGCAAACTGCGTCACCCGAGCCGTTCt                      <  2:4720541/94‑1 (MQ=255)
                                                                    ggAAGAAGTGGGTAAACAGTTCGGCGTTACCCGCGAACGTATCCGTCAGATCGAAGCGAAGGCGCTGCGCAAACTGCGTCACCCGAGCCGTTCt                      <  2:5118843/94‑1 (MQ=255)
                                                                    ggAAGAAGTGGGTAAACAGTTCGGCGTTACCCGCGAACGTATCCGTCAGAGCGAAGCGAAGGCGCTGCGCAAACTGCGACACCCGAGCCGTGCt                      >  1:9420299/1‑94 (MQ=255)
                                                                     gaagaaGTGGGTAAACAGTTCGGCGTTACCCGCGAACGTATCCGTCAGATCGAAGCGAAGGCGCTGCGCAAACTGCGTCACCCGAGCCGTTCTg                     >  1:9060584/1‑94 (MQ=255)
                                                                       agaagTGGGTAAACAGTTCGGCTTCACCCGCGAACGTATCCGTCAGATCGAAGCGAAGGCGCTGCGCAAACTGCTTCACCCGAGCCGTTCTGaa                   <  1:2407855/94‑1 (MQ=255)
                                                                        gaagTGGGTAAACAGTTCGGCGTTACCCGCGAACGTATCCGTCAGATCGAAGCGAAGGCGCTGCGCAAACTGCGTCACCCGAGCCGTTCTGAAg                  >  1:2943935/1‑94 (MQ=255)
                                                                        gaagTGGGTAAAAAGTTCGACGTTACCCGCGAACGTATCCGTCAGATCGAAGCGAAGGCGCTGCGCAAACTGCGTCACCCGAGCCGTTCTGAAg                  <  1:5136724/94‑1 (MQ=255)
                                                                        aaagTGGGTAAACAGTTCGGCGTTACCCGCGAACGTATCCGTCAGATCGAAGCGAAGGCGCTGCGCAAACTGCGTCACCCGAGCCGTTCTGAAg                  <  2:326325/93‑1 (MQ=255)
                                                                         aagTGGGTAAACAGTTCGGCGTTCCCCGCGAACGTATCCGTCAGATCGAAGCGAAGGCGCGGCGCAAACTGCGTCCCCCGAGCCGTTCTGAAGt                 <  1:9763466/94‑1 (MQ=255)
                                                                           gTGGGTAAACAGTTCGGCGTTACCCGCGAACGTATCCGTCAGATCGAAGCGAAGGCGCTGCGCAAACTGCGTCACCCGAGCCGTTCTGAAGtgc               >  2:7731878/1‑94 (MQ=255)
                                                                            tGGGTAAACAGTTCGGCGTTACCCGCGAACGTATCCGTCAGATCGAAGCGAAGGCGCTGCGCAAACTGCGTCACCCGAGCCGTTCTGAAggcct              >  2:254701/1‑90 (MQ=255)
                                                                             gggTAAACAGTTCGGCGTTACCCGCGAACGTATCCGTCAGATCGAAGCGAAGGCGCTGCGCAAACTGCGTCACCCGAGCCGTTCTGAAGtgctg             <  1:3518257/94‑1 (MQ=255)
                                                                              ggTAAACAGTTCGGCGTTACCCGCGAACGTATCCGTCAGATCGAAGCGAAGGCGCTGCGCAAACTGCGTCACCCGAGCCGTTCTGAAGtgctgc            <  1:10137334/94‑1 (MQ=255)
                                                                              ggTAAACAGTTCGGCGTTACCCGCGAACGTATCCGTCAGATCGAAGCGAAGGCGCTGCGCAAACTGCGTCACCCGAGCCGTTCTGAAGtgctgc            <  1:1000544/94‑1 (MQ=255)
                                                                                 aaaCAGTTCGGCGTTACCCGCGAACGTATCCGTCAGATCGAAGCGAAGGCGCTGCGCAAACTGCGTCACCCGAGCCGTTCTGAAGTGCTGCGTa         >  2:62324/1‑94 (MQ=255)
                                                                                  aaCAGTTCGGCGTTACCCGCGAACGTATCCGTCAGATCGAAGCGAAGGCGCTGCGCAAACTGCGTCACCCGAGCCGTTCTGAAGTGCTGCGTAg        >  2:721876/1‑94 (MQ=255)
                                                                                  aaCAGTTCGGCGTTACCCGCGAACGTATCCGTCAGATCGAAGCGAAGGCGCTGCGCAAACTGCGTCACCCGAGCCGTTCTGAAGTGCTGCGTAg        >  1:2784894/1‑94 (MQ=255)
                                                                                  aaCAGTTCGGCGTTACCCGCGAACGTATCCGTCAGATCGAAGCGAAGGCGCTGCGCAAACTGCGTCACCCGAGCCGTTCTGAAGTGCTGCGTAg        <  1:10220889/94‑1 (MQ=255)
                                                                                   aCAGTTCGGCGTTACCCGCGAACGTATCCGTCAGATCGAAGCGAAGGCGCTGCGCAAACTGCGTCACCCGAGCCGTTCTGAAGTGCTGCGTAGc       <  1:8412781/94‑1 (MQ=255)
                                                                                   aCAGTTCGGCGTTACCCGCGAACGTATCCGTCAGATCGAAGCGAAGGCGCTGCGCAAACTGCGTCACCCGAGCCGTTCTGAAGTGCTGCGTAGc       >  2:626098/1‑94 (MQ=255)
                                                                                    cAGTTCGGCGTTACCCGCGAACGTATCCGTCAGATCGAAGCGAAGGCGCTGCGCAAACTGCGTCACCCGAGCCGTTCTGAAGTGCTGCGTAGCt      >  2:4095723/1‑94 (MQ=255)
                                                                                    cAGTTCGGCGTTACCCGCGAACGTATCCGTCAGATCGAAGCGAAGGCGCTGCGCAAACTGCGTCACCCGAGCCGTTCTGAAGTGCTGCGTAGCt      <  2:4909425/94‑1 (MQ=255)
                                                                                      gTTCGCCGTTACCCGCGAACGTATCCGTCAGATCGAAGCGAAGGCGCTGCGCAAACTGCGTCACCCGAGCCGTTCTGAAGTGCTGCGTAGCTTc    <  1:6355996/94‑1 (MQ=255)
                                                                                       ttCGGCGTTACCCGCGAACGTATCCGTCAGATCGAAGCGAAGGCGCTGCGCAAACTGCGTCACCCGAGCCGTTCTGAAGTGCTGCGTAGCTTcc   >  2:10503955/1‑94 (MQ=255)
                                                                                        tCGGCGTTACCCGCGAACGTATCCGTCAGATCGAAGCGAAGGCGCTGCGCAAACTGCGTCACCCGAGCCGTTCTGAAGTGCTGCGTAGCTTCCt  <  2:7957616/94‑1 (MQ=255)
                                                                                        tCGGCGTTACCCGCGAACGTATCCGTCAGATCGAAGCGAAGGCGCTGCGCAAACTGCGTCACCCGAGCCGTTCTGAAGTGCTGCGTAGCTTCCt  <  1:5756224/94‑1 (MQ=255)
                                                                                           |                                                                                          
CTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTGGGTAAACAGTTCGACGTTACCCGCGAACGTATCCGTCAGATCGAAGCGAAGGCGCTGCGCAAACTGCGTCACCCGAGCCGTTCTGAAGTGCTGCGTAGCTTCCT  >  NC_000913/3212338‑3212519

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 241 total aligned reads displayed.
TCACGACGCTGGAATATACCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCACGTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACG  >  NC_000913/3221004‑3221186
                                                                                             |                                                                                         
TCACGACGCTGGAATATACCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAG                                                                                           <  SRR4302545.6248116/94‑1 (MQ=60)
     ACGCTGGAATATACCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTG                                                                                      <  SRR4302545.7932296/94‑1 (MQ=60)
     ACGCTGGAATATACCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTG                                                                                      <  SRR4302545.810915/94‑1 (MQ=60)
     ACGCTGGAATATACCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTG                                                                                      <  SRR4302545.2262345/94‑1 (MQ=60)
      CGCTGGAATATACCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGC                                                                                     >  SRR4302545.3329718/1‑94 (MQ=60)
       GCTGGAATATACCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCT                                                                                    >  SRR4302545.3665103/1‑94 (MQ=60)
       GCTGGAATATACCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCT                                                                                    >  SRR4302545.22188/1‑94 (MQ=60)
       GCTGGAATATACCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCT                                                                                    >  SRR4302545.1321477/1‑94 (MQ=60)
        CTGGAATATACCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTT                                                                                   >  SRR4302545.88019/1‑94 (MQ=60)
         TGGAATATACCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTT                                                                                  >  SRR4302545.9841180/1‑94 (MQ=60)
         TGGAATATACCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTT                                                                                  <  SRR4302545.7052492/94‑1 (MQ=60)
          GGAATATACCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTT                                                                                 <  SRR4302545.2843869/94‑1 (MQ=60)
            AATATACCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTAC                                                                               <  SRR4302545.8335898/94‑1 (MQ=60)
             ATATGCCCTGTTTGGTGGTGATCGGGTGGTGCACAGCAGCGCCATTGATCATTTGTCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTGCT                                                                              <  SRR4302545.9023242/94‑1 (MQ=57)
             ATATACCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACT                                                                              <  SRR4302545.9142375/94‑1 (MQ=60)
             ATATACCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACT                                                                              >  SRR4302545.8873704/1‑94 (MQ=60)
             ATATACCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACT                                                                              <  SRR4302545.2301164/94‑1 (MQ=60)
             ATATACCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACT                                                                              >  SRR4302545.6978929/1‑94 (MQ=60)
              TATACCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTG                                                                             <  SRR4302545.9317490/94‑1 (MQ=60)
               ATACCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGT                                                                            >  SRR4302545.6592355/1‑94 (MQ=60)
               ATACCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGT                                                                            <  SRR4302545.4668169/94‑1 (MQ=60)
                TACCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTC                                                                           >  SRR4302545.7753146/1‑94 (MQ=60)
                TACCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTC                                                                           >  SRR4302545.1792114/1‑94 (MQ=60)
                TACCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTC                                                                           >  SRR4302545.6975608/1‑94 (MQ=60)
                TACCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTC                                                                           <  SRR4302545.7350596/94‑1 (MQ=60)
                TACCCTGTTTGATGGCGAACGCGCGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTC                                                                           >  SRR4302545.963391/1‑94 (MQ=60)
                 TCCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCG                                                                          <  SRR4302545.5727252/94‑1 (MQ=60)
                 TCCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCG                                                                          <  SRR4302545.2817294/94‑1 (MQ=60)
                 ACCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCG                                                                          <  SRR4302545.8289890/94‑1 (MQ=60)
                   CCTGTTTGATGGCGAACGCGTGGTGGCCAGCAGCGCAATTGATCATTTGGCAATTGAAAAACTGAACAGCGCCAGGTTTGTTTTTACTGTCGAA                                                                        >  SRR4302545.5560337/1‑94 (MQ=60)
                   CCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAA                                                                        <  SRR4302545.7201957/94‑1 (MQ=60)
                    CTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAAC                                                                       <  SRR4302545.4557828/94‑1 (MQ=60)
                    CTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAAC                                                                       <  SRR4302545.6411725/94‑1 (MQ=60)
                    CTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAAC                                                                       <  SRR4302545.7831517/94‑1 (MQ=60)
                     TGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACA                                                                      <  SRR4302545.7766501/94‑1 (MQ=60)
                     TGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACA                                                                      <  SRR4302545.370443/94‑1 (MQ=60)
                     TGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACA                                                                      >  SRR4302545.4552885/1‑94 (MQ=60)
                     TGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACA                                                                      <  SRR4302545.3270395/94‑1 (MQ=60)
                      GTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAG                                                                     <  SRR4302545.1520233/94‑1 (MQ=60)
                        TTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCC                                                                   <  SRR4302545.8688261/94‑1 (MQ=60)
                        TTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCC                                                                   >  SRR4302545.9551973/1‑94 (MQ=60)
                        TTGATGGCGAACCCGCGGGGCACAGCAGCGCCATTGATCATTTGGCCATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCC                                                                   >  SRR4302545.9468397/1‑94 (MQ=60)
                         TGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCG                                                                  >  SRR4302545.7753423/1‑94 (MQ=60)
                         TGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCG                                                                  >  SRR4302545.6577490/1‑94 (MQ=60)
                         TGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCG                                                                  >  SRR4302545.925350/1‑94 (MQ=60)
                         TGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCG                                                                  <  SRR4302545.10883150/94‑1 (MQ=60)
                          GATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGC                                                                 >  SRR4302545.2201690/1‑94 (MQ=60)
                          GATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGC                                                                 >  SRR4302545.1214472/1‑94 (MQ=60)
                          GATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGC                                                                 <  SRR4302545.10391914/94‑1 (MQ=60)
                          GATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGC                                                                 >  SRR4302545.379520/1‑94 (MQ=60)
                          GATCGCGAACGCGTGGTGCACAGCAGCGCCAGTGATCATTTGGCAATTGAACAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGC                                                                 <  SRR4302545.10691504/94‑1 (MQ=60)
                           GTGGCGAACGCGTGGTGCGCAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCA                                                                <  SRR4302545.1693731/94‑1 (MQ=60)
                           ATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCA                                                                <  SRR4302545.3201342/94‑1 (MQ=60)
                           ATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCA                                                                >  SRR4302545.249894/1‑94 (MQ=60)
                           ATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCA                                                                >  SRR4302545.2821873/1‑94 (MQ=60)
                           ATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCA                                                                >  SRR4302545.9451462/1‑94 (MQ=60)
                            TGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAG                                                               >  SRR4302545.679790/1‑94 (MQ=60)
                            TGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTAACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAG                                                               <  SRR4302545.8997558/94‑1 (MQ=60)
                             GGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGC                                                              <  SRR4302545.3263302/94‑1 (MQ=60)
                             GGCGAACGCGTGGTGCACAGCAGCACCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGC                                                              <  SRR4302545.10585592/94‑1 (MQ=60)
                              GCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCA                                                             <  SRR4302545.3725755/94‑1 (MQ=60)
                              GCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCA                                                             <  SRR4302545.208216/94‑1 (MQ=60)
                               CGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAA                                                            >  SRR4302545.6045626/1‑94 (MQ=60)
                                gaagtcggGGTGCACAGCAGCGCCATTGATCATGTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGTTTTGACTGGCGAACAGCCGCAGCAAT                                                           <  SRR4302545.404181/86‑1 (MQ=60)
                                GAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCTCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAAT                                                           >  SRR4302545.1514266/1‑94 (MQ=60)
                                GAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAAT                                                           <  SRR4302545.404182/94‑1 (MQ=60)
                                GAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAAT                                                           <  SRR4302545.7297966/94‑1 (MQ=60)
                                GAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAAT                                                           >  SRR4302545.6787477/1‑94 (MQ=60)
                                GAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAAT                                                           >  SRR4302545.6787478/1‑94 (MQ=60)
                                 AACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATG                                                          <  SRR4302545.3040601/94‑1 (MQ=60)
                                 AACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATG                                                          <  SRR4302545.7643667/94‑1 (MQ=60)
                                 AACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATG                                                          <  SRR4302545.3580764/94‑1 (MQ=60)
                                  ACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGG                                                         <  SRR4302545.1366575/94‑1 (MQ=60)
                                    GCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTC                                                       <  SRR4302545.8456224/94‑1 (MQ=60)
                                    GCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTC                                                       >  SRR4302545.6141379/1‑94 (MQ=60)
                                    GCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTC                                                       <  SRR4302545.2113885/94‑1 (MQ=60)
                                    GCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTC                                                       <  SRR4302545.1299100/94‑1 (MQ=60)
                                     CGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCA                                                      >  SRR4302545.5634171/1‑94 (MQ=60)
                                     CGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCA                                                      <  SRR4302545.7593412/94‑1 (MQ=60)
                                     CGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCA                                                      <  SRR4302545.1401826/94‑1 (MQ=60)
                                     CGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCA                                                      <  SRR4302545.1401827/94‑1 (MQ=60)
                                     CGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCA                                                      <  SRR4302545.3222470/94‑1 (MQ=60)
                                     CGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCA                                                      >  SRR4302545.9237362/1‑94 (MQ=60)
                                     CGTGGTGCACAGCAGCGCCATTGATCATTGGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCA                                                      <  SRR4302545.661075/94‑1 (MQ=60)
                                      GTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAG                                                     <  SRR4302545.3135398/94‑1 (MQ=60)
                                       TGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGC                                                    <  SRR4302545.893022/94‑1 (MQ=60)
                                       TGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGC                                                    <  SRR4302545.10190595/94‑1 (MQ=60)
                                       TGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGC                                                    >  SRR4302545.3918071/1‑94 (MQ=60)
                                       TGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGC                                                    <  SRR4302545.1148240/94‑1 (MQ=60)
                                       TGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGC                                                    <  SRR4302545.1598077/94‑1 (MQ=60)
                                        GGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCA                                                   <  SRR4302545.8482979/94‑1 (MQ=60)
                                        GGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCA                                                   <  SRR4302545.1864138/94‑1 (MQ=60)
                                        GGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCA                                                   <  SRR4302545.9293520/94‑1 (MQ=60)
                                          GGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGA                                                 <  SRR4302545.3169609/94‑1 (MQ=60)
                                           GCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAA                                                <  SRR4302545.957218/94‑1 (MQ=60)
                                            CACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGCAT                                               >  SRR4302545.9292124/1‑94 (MQ=60)
                                            CACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAAT                                               >  SRR4302545.7097719/1‑94 (MQ=60)
                                             ACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATC                                              <  SRR4302545.9555346/94‑1 (MQ=60)
                                             ACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATC                                              >  SRR4302545.2594774/1‑94 (MQ=60)
                                             ACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATC                                              >  SRR4302545.5785990/1‑94 (MQ=60)
                                             ACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATC                                              <  SRR4302545.7672222/94‑1 (MQ=60)
                                               AGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCC                                            >  SRR4302545.2444153/1‑94 (MQ=60)
                                               AGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCC                                            <  SRR4302545.9454399/94‑1 (MQ=60)
                                               AGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGAAGCAATGGTCAGCAGAATCCC                                            >  SRR4302545.10768160/1‑94 (MQ=60)
                                                GCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCC                                           <  SRR4302545.5701084/94‑1 (MQ=60)
                                                 CAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCT                                          <  SRR4302545.8479409/94‑1 (MQ=60)
                                                 CAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCT                                          <  SRR4302545.9822910/94‑1 (MQ=60)
                                                 CAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCT                                          >  SRR4302545.8954223/1‑94 (MQ=60)
                                                  AGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTT                                         >  SRR4302545.6624139/1‑94 (MQ=60)
                                                  AGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTT                                         <  SRR4302545.4889057/94‑1 (MQ=60)
                                                  AGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTT                                         <  SRR4302545.7455377/94‑1 (MQ=60)
                                                  AGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTT                                         >  SRR4302545.7807943/1‑94 (MQ=60)
                                                   GCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTA                                        <  SRR4302545.3168213/94‑1 (MQ=60)
                                                   GCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTA                                        <  SRR4302545.1945029/94‑1 (MQ=60)
                                                   GCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTA                                        <  SRR4302545.650844/94‑1 (MQ=60)
                                                   GCGCCATTGATCATGTGGCAATTGAAAACCTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTA                                        <  SRR4302545.5532460/94‑1 (MQ=60)
                                                    CGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTAT                                       >  SRR4302545.6980001/1‑94 (MQ=60)
                                                     GCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATC                                      >  SRR4302545.1571368/1‑94 (MQ=60)
                                                      CCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCT                                     >  SRR4302545.1359541/1‑94 (MQ=60)
                                                      CCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCT                                     >  SRR4302545.10479943/1‑94 (MQ=60)
                                                      CCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCT                                     >  SRR4302545.3908819/1‑94 (MQ=60)
                                                      CCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCT                                     >  SRR4302545.3908817/1‑94 (MQ=60)
                                                      CCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCT                                     >  SRR4302545.3629923/1‑94 (MQ=60)
                                                       CATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTT                                    >  SRR4302545.7349204/1‑94 (MQ=60)
                                                       CATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTT                                    >  SRR4302545.7294708/1‑94 (MQ=60)
                                                       CATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTT                                    >  SRR4302545.1431519/1‑94 (MQ=60)
                                                       CATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTT                                    <  SRR4302545.9773119/94‑1 (MQ=60)
                                                       CATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTT                                    >  SRR4302545.5107875/1‑94 (MQ=60)
                                                       CATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTT                                    >  SRR4302545.1052889/1‑94 (MQ=60)
                                                       CATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTT                                    >  SRR4302545.3934799/1‑94 (MQ=60)
                                                         TTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTA                                  <  SRR4302545.10697975/94‑1 (MQ=60)
                                                         TTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTA                                  >  SRR4302545.7767034/1‑94 (MQ=60)
                                                          TGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTAC                                 >  SRR4302545.10419611/1‑94 (MQ=60)
                                                          TGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTAC                                 >  SRR4302545.2854088/1‑94 (MQ=60)
                                                          TGAACATTTGGCAATTGAAAAACCTGCCCGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTAC                                 <  SRR4302545.10464801/94‑1 (MQ=60)
                                                           GATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACC                                >  SRR4302545.4029244/1‑94 (MQ=60)
                                                             TCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCAT                              >  SRR4302545.10881691/1‑94 (MQ=60)
                                                              CATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATC                             <  SRR4302545.7103924/94‑1 (MQ=60)
                                                               ATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCT                            <  SRR4302545.2298333/94‑1 (MQ=60)
                                                               ATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCT                            >  SRR4302545.2366884/1‑94 (MQ=60)
                                                               ATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCT                            <  SRR4302545.8566354/94‑1 (MQ=60)
                                                               ATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCT                            <  SRR4302545.4736448/94‑1 (MQ=60)
                                                                ataGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTG                           <  SRR4302545.9380762/91‑1 (MQ=60)
                                                                TTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTTCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTG                           >  SRR4302545.6393054/1‑94 (MQ=60)
                                                                TTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTG                           <  SRR4302545.392790/94‑1 (MQ=60)
                                                                TTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTG                           <  SRR4302545.4614746/94‑1 (MQ=60)
                                                                TTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTG                           <  SRR4302545.9380761/94‑1 (MQ=60)
                                                                 TTTGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGG                          <  SRR4302545.6763840/94‑1 (MQ=60)
                                                                 TTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGG                          >  SRR4302545.6328607/1‑94 (MQ=60)
                                                                  TGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGT                         <  SRR4302545.7180836/94‑1 (MQ=60)
                                                                  TGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGT                         >  SRR4302545.4997881/1‑94 (MQ=60)
                                                                  TGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGT                         <  SRR4302545.6054673/94‑1 (MQ=60)
                                                                   GGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATagatcagagatcgga                        <  SRR4302545.10745265/94‑16 (MQ=60)
                                                                   GGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTC                        <  SRR4302545.753575/94‑1 (MQ=60)
                                                                   GGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTC                        >  SRR4302545.8172291/1‑94 (MQ=60)
                                                                    GCAATTGAAAAACTGACCAGAGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCA                       <  SRR4302545.10701640/94‑1 (MQ=60)
                                                                    GCAATTGAAAAACTGACCACCGCCAGCGTTGCTTTTACTGTCGACCAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCA                       <  SRR4302545.7535183/94‑1 (MQ=60)
                                                                     CAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCAT                      >  SRR4302545.7573677/1‑94 (MQ=60)
                                                                       ATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCCTCTGGTCATGA                    >  SRR4302545.8238752/1‑94 (MQ=60)
                                                                       ATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGA                    <  SRR4302545.101155/94‑1 (MQ=60)
                                                                       ATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGA                    <  SRR4302545.1282395/94‑1 (MQ=60)
                                                                       ATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGA                    <  SRR4302545.5902794/94‑1 (MQ=60)
                                                                       AGTGAAAAACTGGCCAGCGCCAGCTTTGCTTTTACTGTCGATCAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTCTACCATCTGGTCATGA                    <  SRR4302545.101157/94‑1 (MQ=60)
                                                                        TTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGAC                   >  SRR4302545.5749441/1‑94 (MQ=60)
                                                                         TGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACG                  >  SRR4302545.7268785/1‑94 (MQ=60)
                                                                          GAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGC                 >  SRR4302545.2587547/1‑94 (MQ=60)
                                                                          GAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGC                 <  SRR4302545.5042120/94‑1 (MQ=60)
                                                                           AAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCT                >  SRR4302545.9747705/1‑94 (MQ=60)
                                                                           AAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCT                <  SRR4302545.8030070/94‑1 (MQ=60)
                                                                            AAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTG               <  SRR4302545.10412707/94‑1 (MQ=60)
                                                                              ATCTGACCAGCTCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAA             <  SRR4302545.10500374/94‑1 (MQ=60)
                                                                              AACTGCCCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAA             <  SRR4302545.5566859/94‑1 (MQ=60)
                                                                              AACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAA             >  SRR4302545.3714310/1‑94 (MQ=60)
                                                                              AACTGACCAGCGCCAGCTGTGCTTTTCCTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAA             <  SRR4302545.10500373/94‑1 (MQ=60)
                                                                               ACTGACCAGCGCCAGCTTTGCTTTTACCGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAA            >  SRR4302545.4819414/1‑94 (MQ=60)
                                                                                CTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCGGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAG           >  SRR4302545.9455459/1‑94 (MQ=60)
                                                                                CTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAG           >  SRR4302545.230663/1‑94 (MQ=60)
                                                                                 TGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGA          >  SRR4302545.1680929/1‑94 (MQ=60)
                                                                                  GACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGAC         <  SRR4302545.5573383/94‑1 (MQ=60)
                                                                                  GACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGAC         >  SRR4302545.6035529/1‑94 (MQ=60)
                                                                                  GACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGAC         <  SRR4302545.2284830/94‑1 (MQ=60)
                                                                                   ACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACG        <  SRR4302545.10152991/94‑1 (MQ=60)
                                                                                   ACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACG        >  SRR4302545.5515299/1‑94 (MQ=60)
                                                                                   ACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACG        >  SRR4302545.3077658/1‑94 (MQ=60)
                                                                                   ACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACG        <  SRR4302545.6735692/94‑1 (MQ=60)
                                                                                   ACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACG        >  SRR4302545.231435/1‑94 (MQ=60)
                                                                                    CCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGC       >  SRR4302545.2811030/1‑94 (MQ=60)
                                                                                     CAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCC      >  SRR4302545.5041941/1‑94 (MQ=60)
                                                                                      AGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCA     >  SRR4302545.728497/1‑94 (MQ=60)
                                                                                       GCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAA    >  SRR4302545.9218627/1‑94 (MQ=60)
                                                                                       GCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAA    <  SRR4302545.3541616/94‑1 (MQ=60)
                                                                                       GCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAA    <  SRR4302545.3541622/94‑1 (MQ=60)
                                                                                        CGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAAC   <  SRR4302545.2446599/94‑1 (MQ=60)
                                                                                        CGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAAC   <  SRR4302545.8722548/94‑1 (MQ=60)
                                                                                        CGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAAC   >  SRR4302545.10128594/1‑94 (MQ=60)
                                                                                        CGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAAC   >  SRR4302545.8171595/1‑94 (MQ=60)
                                                                                         GCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACG  >  SRR4302545.2029631/1‑94 (MQ=60)
                                                                                         GCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACG  >  SRR4302545.10431508/1‑94 (MQ=60)
                                                                                         GCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACG  >  SRR4302545.5613169/1‑94 (MQ=60)
                                                                                         GCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACG  <  SRR4302545.10004433/94‑1 (MQ=60)
                                                                                             |                                                                                         
TCACGACGCTGGAATATACCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCACGTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACG  >  NC_000913/3221004‑3221186

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑