Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 647 270.1 21258240 98.4% 20918108 94.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,222,944 4 bp→AGCA coding (2671‑2674/3093 nt) b3076 → evolved beta‑D‑galactosidase alpha subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,222,9440CA100.0% 1439.1 / NA 375Q891S (CAA→AGC) b3076evolved beta‑D‑galactosidase alpha subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (178/197);  total (178/197)
*NC_0009133,222,9450AG99.7% 1286.7 / NA 375Q891S (CAA→AGC) b3076evolved beta‑D‑galactosidase alpha subunit
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (178/196);  minor base T (0/1);  total (178/197)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*NC_0009133,222,9460AC99.5% 1273.1 / ‑5.6 375Q891S (CAA→AGC) b3076evolved beta‑D‑galactosidase alpha subunit
Reads supporting (aligned to +/- strand):  ref base A (1/1);  new base C (177/196);  total (178/197)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*NC_0009133,222,9470GA99.5% 1349.3 / NA 374A892T (GCC→ACC) b3076evolved beta‑D‑galactosidase alpha subunit
Reads supporting (aligned to +/- strand):  ref base G (0/1);  major base A (177/194);  minor base T (0/2);  total (177/197)
Fisher's exact test for biased strand distribution p-value = 5.00e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 379 total aligned reads displayed.

CGATCAGGTGGCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCCAAGCCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGACGGCACTGACTAACCGCCACG  >  NC_000913/3222862‑3223037
                                                                                  ||||                                                                                          
cGATCAGGTGGCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGAt                                                                                    <  2:4764494/94‑1 (MQ=255)
 gATCAGGTGGCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATg                                                                                   >  1:7836468/1‑94 (MQ=255)
 gATCAGGTGGCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATg                                                                                   >  1:1785043/1‑94 (MQ=255)
  aTCAGGTGGCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGc                                                                                  >  2:4332036/1‑94 (MQ=255)
   tCAGGTGGCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGcc                                                                                 <  2:3068089/94‑1 (MQ=255)
    cAGGTGGCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCa                                                                                >  2:4702289/1‑94 (MQ=255)
     aGGTGGCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCAt                                                                               >  2:8744806/1‑94 (MQ=255)
      ggTGGCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATg                                                                              >  1:6236043/1‑94 (MQ=255)
      ggTGGCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATg                                                                              >  2:8633966/1‑94 (MQ=255)
        tGGCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGtt                                                                            >  1:425508/1‑94 (MQ=255)
        tGGCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGtt                                                                            >  2:5297483/1‑94 (MQ=255)
         ggCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTc                                                                           <  1:10434284/94‑1 (MQ=255)
          gCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCg                                                                          <  1:9316451/94‑1 (MQ=255)
          gCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCg                                                                          >  1:7042424/1‑94 (MQ=255)
            gTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCgag                                                                        <  1:3319426/94‑1 (MQ=255)
            gTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCgag                                                                        >  2:3686129/1‑94 (MQ=255)
            gTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCgag                                                                        <  2:3219471/94‑1 (MQ=255)
            gTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCgag                                                                        <  1:10106918/94‑1 (MQ=255)
            gTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGGTCgag                                                                        >  1:5422427/1‑94 (MQ=255)
             tATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCgaga                                                                       <  2:9281191/94‑1 (MQ=255)
                tACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACt                                                                    >  2:4561545/1‑94 (MQ=255)
                 aCGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTa                                                                   <  1:1945819/94‑1 (MQ=255)
                  cGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTAt                                                                  <  1:2197400/94‑1 (MQ=255)
                  cGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTAt                                                                  >  1:1178274/1‑94 (MQ=255)
                  cGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTAt                                                                  <  2:2248588/94‑1 (MQ=255)
                  cGGTCGTGAGCCGAGCGAAAACTATGACGACAGACAGTAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTAt                                                                  <  1:288086/94‑1 (MQ=255)
                   ggTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATc                                                                 <  2:3798509/94‑1 (MQ=255)
                    gTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATcc                                                                >  2:4873581/1‑94 (MQ=255)
                     tCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATccc                                                               >  1:9104926/1‑94 (MQ=255)
                      cGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCt                                                              >  2:1653194/1‑94 (MQ=255)
                      cGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCt                                                              <  1:3112121/94‑1 (MQ=255)
                       gTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCtt                                                             >  2:2480208/1‑94 (MQ=255)
                       gTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCtt                                                             >  1:767972/1‑94 (MQ=255)
                       gTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCtt                                                             >  2:8977667/1‑94 (MQ=255)
                        tGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTc                                                            >  1:1667151/1‑94 (MQ=255)
                        tGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTc                                                            <  1:4838862/94‑1 (MQ=255)
                         ggACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTcc                                                           >  2:5262458/1‑94 (MQ=255)
                         ggACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTcc                                                           <  2:69566/94‑1 (MQ=255)
                         ggACCGGGCGAAAACTACGCCGACAGCCAGAAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTcc                                                           <  2:4944928/94‑1 (MQ=255)
                           aCCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCg                                                         <  2:7252342/94‑1 (MQ=255)
                              gggCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAg                                                      >  1:7172375/1‑94 (MQ=255)
                                 cGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGaac                                                   <  2:4290635/94‑1 (MQ=255)
                                 cGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGaac                                                   <  1:4701368/94‑1 (MQ=255)
                                  gAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGaaca                                                  <  1:2005655/94‑1 (MQ=255)
                                   aaaaCTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGaacaa                                                 >  1:4001622/1‑94 (MQ=255)
                                    aaccTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGaacaac                                                <  1:6494031/91‑1 (MQ=255)
                                    aaaCTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGaacaac                                                >  2:5578168/1‑94 (MQ=255)
                                     aaCTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACg                                               >  1:3533987/1‑94 (MQ=255)
                                     aaCTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACg                                               <  1:2979603/94‑1 (MQ=255)
                                     aaCTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACg                                               >  1:4583400/1‑94 (MQ=255)
                                      aCTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACgg                                              <  2:9738489/94‑1 (MQ=255)
                                       cTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGt                                             <  2:6510744/94‑1 (MQ=255)
                                       cTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTAGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGt                                             >  1:521453/1‑94 (MQ=255)
                                          cGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAAc                                          >  1:6675507/1‑94 (MQ=255)
                                           gCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAAcc                                         <  1:1319297/94‑1 (MQ=255)
                                           gCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAAcc                                         <  1:8915222/94‑1 (MQ=255)
                                           gCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAAcc                                         <  1:2354932/94‑1 (MQ=255)
                                           cccGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAAcc                                         <  2:1969720/93‑1 (MQ=255)
                                            ccGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCg                                        <  1:7473724/94‑1 (MQ=255)
                                             cGACCGCCAGGAGGCTAACAGCATCGATATCTGGCGCAGCACCGGCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGt                                       <  2:8155694/94‑1 (MQ=255)
                                             cGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGt                                       >  2:9084499/1‑94 (MQ=255)
                                              gACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTc                                      <  2:7752498/94‑1 (MQ=255)
                                              gACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTc                                      <  2:7150146/94‑1 (MQ=255)
                                              gACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGAACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTc                                      <  1:9408264/94‑1 (MQ=255)
                                                cagccagcAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAg                                    <  2:6091444/94‑1 (MQ=255)
                                                 agccagcAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGc                                   >  2:10328898/1‑94 (MQ=255)
                                                     agcagGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGt                               >  1:9576968/1‑94 (MQ=255)
                                                     agcagGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGt                               >  2:7596437/1‑94 (MQ=255)
                                                     agcagGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGt                               <  1:10607822/94‑1 (MQ=255)
                                                      gcagGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTc                              <  2:5076695/94‑1 (MQ=255)
                                                      gcagGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTc                              <  2:1005449/94‑1 (MQ=255)
                                                       cagGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTcc                             >  1:5902110/1‑94 (MQ=255)
                                                       cagGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTcc                             >  2:1241372/1‑94 (MQ=255)
                                                        agGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCg                            <  2:5428825/94‑1 (MQ=255)
                                                        agGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCg                            >  1:9782111/1‑94 (MQ=255)
                                                        agGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCg                            >  1:2700754/1‑94 (MQ=255)
                                                          gCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCt                          <  2:9357160/94‑1 (MQ=255)
                                                           cTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTg                         >  2:4797799/1‑94 (MQ=255)
                                                           cTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTg                         <  1:6291700/94‑1 (MQ=255)
                                                           cTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTg                         >  2:1329105/1‑94 (MQ=255)
                                                           cTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTg                         <  1:7950378/94‑1 (MQ=255)
                                                            tAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCGTTCCCGCAGAACAACGGTAACAGTCACCATGTCCGCTgg                        >  2:3510237/1‑94 (MQ=255)
                                                            tAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTgg                        <  1:4125750/94‑1 (MQ=255)
                                                             aaCATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGa                       <  1:10054195/94‑1 (MQ=255)
                                                             aaCATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGa                       >  2:2128297/1‑94 (MQ=255)
                                                              aCATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGAc                      <  1:8527345/94‑1 (MQ=255)
                                                              aCATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGAc                      >  1:8147862/1‑94 (MQ=255)
                                                              aCATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGAc                      <  1:9952435/94‑1 (MQ=255)
                                                                atcatcGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGACgg                    >  2:5937654/1‑94 (MQ=255)
                                                                atcatcGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGACgg                    <  2:8465555/94‑1 (MQ=255)
                                                                atcatcGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGACgg                    >  2:4485343/1‑94 (MQ=255)
                                                                  catcGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGACGGCa                  <  2:624115/94‑1 (MQ=255)
                                                                    tcGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGACGGCACt                <  1:6737518/94‑1 (MQ=255)
                                                                     cGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGACGGCACTg               >  2:10441209/1‑94 (MQ=255)
                                                                       atatCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGACGGCACTGAc             <  1:4473057/94‑1 (MQ=255)
                                                                       atatCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGACGGCACTCAc             >  1:1592616/1‑94 (MQ=255)
                                                                        tatCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGACGGCACTGACt            <  1:3665097/94‑1 (MQ=255)
                                                                        tatCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGACGGCACTGACt            <  1:10256556/94‑1 (MQ=255)
                                                                        tatCTGGCGCAGCACCGTCGATGCCATGTTCGACAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGACGGCACTGACt            <  2:7403620/94‑1 (MQ=255)
                                                                         atCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGACGGCACTGACTa           <  2:8070261/94‑1 (MQ=255)
                                                                                  ||||                                                                                          
CGATCAGGTGGCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCCAAGCCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGACGGCACTGACTAACCGCCACG  >  NC_000913/3222862‑3223037

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 257 total aligned reads displayed.
CGCAACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGCGGCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTAT‑CCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTA  >  NC_000913/3224786‑3224970
                                                                                             |                                                                                            
CGCAACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGCGC                                                                                              >  SRR4302545.10081808/1‑94 (MQ=60)
CGCAACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGCGC                                                                                              <  SRR4302545.3863425/94‑1 (MQ=60)
CGCAACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGCGC                                                                                              >  SRR4302545.4032595/1‑94 (MQ=60)
CGCAACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGCGC                                                                                              >  SRR4302545.8863218/1‑94 (MQ=60)
CGCAACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGCGC                                                                                              <  SRR4302545.8583328/94‑1 (MQ=60)
  CAACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGC                                                                                           <  SRR4302545.5951690/94‑1 (MQ=60)
   AACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCA                                                                                          >  SRR4302545.1418593/1‑94 (MQ=60)
   AACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCA                                                                                          >  SRR4302545.1222329/1‑94 (MQ=60)
    ACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAG                                                                                         >  SRR4302545.6138754/1‑94 (MQ=60)
      GCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCG                                                                                       >  SRR4302545.2980804/1‑94 (MQ=60)
      GCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCG                                                                                       <  SRR4302545.6332445/94‑1 (MQ=60)
       CCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGT                                                                                      <  SRR4302545.1829307/94‑1 (MQ=60)
       CCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGT                                                                                      <  SRR4302545.7782148/94‑1 (MQ=60)
       CCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGT                                                                                      <  SRR4302545.9111416/94‑1 (MQ=60)
        CGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTG                                                                                     <  SRR4302545.2188657/94‑1 (MQ=60)
        CGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGCTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTG                                                                                     <  SRR4302545.8696106/94‑1 (MQ=60)
        CGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGCTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTG                                                                                     <  SRR4302545.8696105/94‑1 (MQ=60)
         GGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGG                                                                                    >  SRR4302545.6538732/1‑94 (MQ=60)
         GGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGG                                                                                    <  SRR4302545.6634534/94‑1 (MQ=60)
          GTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGA                                                                                   >  SRR4302545.2512087/1‑94 (MQ=60)
          GTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGA                                                                                   <  SRR4302545.6337292/94‑1 (MQ=60)
          GTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCAGCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGA                                                                                   >  SRR4302545.7580010/1‑94 (MQ=60)
            GAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATC                                                                                 >  SRR4302545.8595558/1‑94 (MQ=60)
             AAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCC                                                                                <  SRR4302545.6173959/94‑1 (MQ=60)
               AATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAG                                                                              <  SRR4302545.5468639/94‑1 (MQ=60)
                 TTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGCACTGAACGAAAAGGCCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTT                                                                            >  SRR4302545.8319663/1‑94 (MQ=60)
                 TTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTT                                                                            >  SRR4302545.7481104/1‑94 (MQ=60)
                   TTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCC                                                                          >  SRR4302545.364020/1‑94 (MQ=60)
                   TTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCC                                                                          >  SRR4302545.1726013/1‑94 (MQ=60)
                   TTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCC                                                                          <  SRR4302545.2464272/94‑1 (MQ=60)
                   TTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCC                                                                          <  SRR4302545.10867740/94‑1 (MQ=60)
                    TCTTCTCCGAAATCCCGGAAGGCATCTTTTGTAATAAAACCGTCGAACTGAACGAAAACGGCGTTCCGACGC‑GCGCAGCGTGGATCCAGTTCCT                                                                         <  SRR4302545.1663838/94‑1 (MQ=60)
                    TCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCT                                                                         >  SRR4302545.5652271/1‑94 (MQ=60)
                     CTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTG                                                                        <  SRR4302545.4865888/94‑1 (MQ=60)
                     CTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTACGGCGC‑GCGCAGCGTGGATCCAGTTCCTC                                                                        >  SRR4302545.428667/1‑94 (MQ=60)
                     CTTCTCCGAAATCCCGGAAGGCATCTTTGGCAAGAAAACCTTCGAACTGAACGAAAACGGCGGTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTG                                                                        >  SRR4302545.5356155/1‑94 (MQ=60)
                       TCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGAT                                                                      <  SRR4302545.9399824/94‑1 (MQ=60)
                       TCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGAT                                                                      >  SRR4302545.1661332/1‑94 (MQ=60)
                       TCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGAT                                                                      <  SRR4302545.2771157/94‑1 (MQ=60)
                       TCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGAT                                                                      >  SRR4302545.5830230/1‑94 (MQ=60)
                       TCTCCGAAATCCCAGAAGGCATCTTTGGGAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGAT                                                                      <  SRR4302545.9399823/94‑1 (MQ=60)
                        CTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATG                                                                     >  SRR4302545.8628385/1‑94 (MQ=60)
                        CTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATC                                                                     >  SRR4302545.3180287/1‑94 (MQ=60)
                        CTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATC                                                                     <  SRR4302545.7841672/94‑1 (MQ=60)
                           CGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACCGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTACGTGATCGTC                                                                  >  SRR4302545.2784131/1‑94 (MQ=60)
                            GAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCT                                                                 >  SRR4302545.8001251/1‑94 (MQ=60)
                            GAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCA                                                                 >  SRR4302545.3022135/1‑94 (MQ=60)
                            GAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCA                                                                 >  SRR4302545.2652870/1‑94 (MQ=60)
                            GAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCA                                                                 >  SRR4302545.7551523/1‑94 (MQ=60)
                             CAATCCCGGAAGGTATCTTTGGTAACAAAACCGTCGAACTGAACGAAACCGGCGTTCCGGCGC‑GCGCAGCGTGTATCCAGTTCCTCATCTTCAT                                                                <  SRR4302545.6171206/94‑1 (MQ=60)
                             AAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCAT                                                                >  SRR4302545.7213074/1‑94 (MQ=60)
                              AATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGTTCCAGTTCttgaccttcacc                                                               >  SRR4302545.6224646/1‑82 (MQ=60)
                              AATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATC                                                               <  SRR4302545.8901168/94‑1 (MQ=60)
                               ATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCC                                                              <  SRR4302545.895249/94‑1 (MQ=60)
                                 CCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCG                                                            <  SRR4302545.5461920/94‑1 (MQ=60)
                                 CCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCG                                                            <  SRR4302545.8174462/94‑1 (MQ=60)
                                 CCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCG                                                            >  SRR4302545.2607888/1‑94 (MQ=60)
                                 CCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCG                                                            >  SRR4302545.10147281/1‑94 (MQ=60)
                                  CCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGGACGAAAACGGCGGTCCGGCGC‑GCGCAGCGTGGGTCCAGTGCCTGATCtgcatccggc                                                           >  SRR4302545.8366639/1‑84 (MQ=60)
                                  CCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATGGTCATCCCGC                                                           >  SRR4302545.3160914/1‑94 (MQ=60)
                                  CCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGC                                                           >  SRR4302545.3434551/1‑94 (MQ=60)
                                  CCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGC                                                           >  SRR4302545.3209118/1‑94 (MQ=60)
                                  CCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGC                                                           <  SRR4302545.7256498/94‑1 (MQ=60)
                                  CCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGC                                                           <  SRR4302545.7256497/94‑1 (MQ=60)
                                   CGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCT                                                          >  SRR4302545.8356388/1‑94 (MQ=60)
                                   CGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCT                                                          >  SRR4302545.10533627/1‑94 (MQ=60)
                                   CAGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGAGCTCCGAAGC‑GCGAAGCGTGGATCCAGTTCCTGATCGTCATCCCGCT                                                          >  SRR4302545.8415935/1‑94 (MQ=60)
                                    GGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTG                                                         >  SRR4302545.9569548/1‑94 (MQ=60)
                                    GGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTG                                                         >  SRR4302545.9569549/1‑94 (MQ=60)
                                    GGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTG                                                         <  SRR4302545.3768799/94‑1 (MQ=60)
                                    GGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTG                                                         >  SRR4302545.7397235/1‑94 (MQ=60)
                                     GAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGC                                                        >  SRR4302545.5279055/1‑94 (MQ=60)
                                      AAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCATCATCCCGCTGAT                                                       >  SRR4302545.10276692/1‑94 (MQ=60)
                                        GGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGT                                                     >  SRR4302545.8120886/1‑94 (MQ=60)
                                        GGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGA                                                     >  SRR4302545.8120885/1‑94 (MQ=60)
                                        GGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGA                                                     <  SRR4302545.535033/94‑1 (MQ=60)
                                         GCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGAT                                                    <  SRR4302545.10773088/94‑1 (MQ=60)
                                         GCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGAT                                                    <  SRR4302545.963976/94‑1 (MQ=60)
                                         GCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGAT                                                    <  SRR4302545.8167139/94‑1 (MQ=60)
                                          CATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATT                                                   <  SRR4302545.2284455/94‑1 (MQ=60)
                                           ATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTA                                                  <  SRR4302545.10393499/94‑1 (MQ=60)
                                           ATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTA                                                  <  SRR4302545.10393498/94‑1 (MQ=60)
                                           ATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTA                                                  >  SRR4302545.6806154/1‑94 (MQ=60)
                                            TCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTAT                                                 >  SRR4302545.6835198/1‑94 (MQ=60)
                                            TCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTAT                                                 >  SRR4302545.397097/1‑94 (MQ=60)
                                             CTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTAT‑C                                               >  SRR4302545.9735513/1‑94 (MQ=60)
                                              TTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTAT‑CC                                              <  SRR4302545.9480008/94‑1 (MQ=60)
                                              TTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTAT‑CC                                              <  SRR4302545.1570918/94‑1 (MQ=60)
                                               TTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTAT‑CCC                                             >  SRR4302545.3330866/1‑94 (MQ=60)
                                                 GGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTAT‑CCcga                                           >  SRR4302545.9064985/1‑91 (MQ=60)
                                                 GGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTAT‑CCcga                                           >  SRR4302545.6424163/1‑91 (MQ=60)
                                                 GGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTAT‑CCcga                                           >  SRR4302545.6757959/1‑91 (MQ=60)
                                                  GTAAGAAAACCGTCGAACTGAACTAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGAT                                           >  SRR4302545.9273953/1‑94 (MQ=60)
                                                  GTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGAT                                           <  SRR4302545.6294128/94‑1 (MQ=60)
                                                  GTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTAT‑CCcgaa                                          <  SRR4302545.4403694/94‑5 (MQ=60)
                                                   GAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATG                                          <  SRR4302545.3296310/94‑1 (MQ=60)
                                                   GAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATG                                          <  SRR4302545.9051267/94‑1 (MQ=60)
                                                    AAGAAATCCGTCGAACTGAACGAAAGCGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGC                                         <  SRR4302545.3799406/94‑1 (MQ=60)
                                                    AAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGC                                         >  SRR4302545.6774064/1‑94 (MQ=60)
                                                    AAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGC                                         >  SRR4302545.4299645/1‑94 (MQ=60)
                                                    AAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGC                                         >  SRR4302545.7634874/1‑94 (MQ=60)
                                                    AAAAAATCCGTCGAACTGAACGAAAACGTCCTGCCGGCGC‑GCGCAGCGTGGATCCAATTCCTGATCGTCATCCCGCTGATGATTATCCCGATGC                                         <  SRR4302545.1617799/94‑1 (MQ=60)
                                                     AGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCT                                        >  SRR4302545.9397694/1‑94 (MQ=60)
                                                     AGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCT                                        >  SRR4302545.2373817/1‑94 (MQ=60)
                                                     AGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCT                                        >  SRR4302545.9397693/1‑94 (MQ=60)
                                                      GAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTC                                       >  SRR4302545.9811490/1‑94 (MQ=60)
                                                      GAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGAGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTC                                       <  SRR4302545.5939272/94‑1 (MQ=60)
                                                      AAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTGCTGATCGTCATCCCGCTGATGATTATCCCGATGCTC                                       <  SRR4302545.3207602/94‑1 (MQ=60)
                                                       AAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCG                                      <  SRR4302545.1133786/94‑1 (MQ=60)
                                                       AAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCG                                      <  SRR4302545.325657/94‑1 (MQ=60)
                                                        AAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGG                                     >  SRR4302545.3815114/1‑94 (MQ=60)
                                                        AAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGG                                     >  SRR4302545.7564661/1‑94 (MQ=60)
                                                        AAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGG                                     >  SRR4302545.2345004/1‑94 (MQ=60)
                                                         AACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGT                                    >  SRR4302545.4431112/1‑94 (MQ=60)
                                                         AACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGT                                    >  SRR4302545.4439716/1‑94 (MQ=60)
                                                         AACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGT                                    >  SRR4302545.298760/1‑94 (MQ=60)
                                                         AACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGT                                    >  SRR4302545.10217898/1‑94 (MQ=60)
                                                          ACCGTCGAACTGAACTAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCTGTT                                   <  SRR4302545.8415414/94‑1 (MQ=60)
                                                          ACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTT                                   <  SRR4302545.1277317/94‑1 (MQ=60)
                                                          ACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTT                                   >  SRR4302545.5025409/1‑94 (MQ=60)
                                                          ACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTT                                   <  SRR4302545.10934295/94‑1 (MQ=60)
                                                          ACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTT                                   >  SRR4302545.2083/1‑94 (MQ=60)
                                                            CGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCC                                 <  SRR4302545.4143457/94‑1 (MQ=60)
                                                            CGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCC                                 >  SRR4302545.9175605/1‑94 (MQ=60)
                                                             GTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCA                                >  SRR4302545.5861305/1‑94 (MQ=60)
                                                             GTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCA                                <  SRR4302545.898541/94‑1 (MQ=60)
                                                             GTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCA                                >  SRR4302545.9890764/1‑94 (MQ=60)
                                                              TCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAA                               >  SRR4302545.6455627/1‑94 (MQ=60)
                                                              TCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAA                               >  SRR4302545.1091585/1‑94 (MQ=60)
                                                              TCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAA                               <  SRR4302545.10278759/94‑1 (MQ=60)
                                                              TCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAA                               <  SRR4302545.7913282/94‑1 (MQ=60)
                                                               CGAGCTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAAT                              <  SRR4302545.733848/94‑1 (MQ=60)
                                                               CGAACTGAACGAAAACGGCGTTCGGGCTC‑GCGCAGCGTGGTTCCAGGTCCTGATCGTCATCCCGCTGTTGATTATtccgaccctcgcttcacgt                              >  SRR4302545.2030282/1‑75 (MQ=48)
                                                               CGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAAT                              <  SRR4302545.800389/94‑1 (MQ=60)
                                                               CGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAAT                              >  SRR4302545.2847356/1‑94 (MQ=60)
                                                                GAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATA                             >  SRR4302545.1065640/1‑94 (MQ=60)
                                                                 AACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATAC                            <  SRR4302545.7996002/94‑1 (MQ=60)
                                                                  ACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACC                           <  SRR4302545.1021072/94‑1 (MQ=60)
                                                                  ACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACC                           >  SRR4302545.4079999/1‑94 (MQ=60)
                                                                  ACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACC                           <  SRR4302545.3241133/94‑1 (MQ=60)
                                                                   CTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCG                          <  SRR4302545.3502686/94‑1 (MQ=60)
                                                                   CTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCG                          <  SRR4302545.3502687/94‑1 (MQ=60)
                                                                   CTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCG                          <  SRR4302545.3151042/94‑1 (MQ=60)
                                                                   CTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCG                          >  SRR4302545.3670005/1‑94 (MQ=60)
                                                                    TGAACGCAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGCTCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGT                         <  SRR4302545.9677496/94‑1 (MQ=60)
                                                                    TGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGT                         >  SRR4302545.2396023/1‑94 (MQ=60)
                                                                    TGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGT                         <  SRR4302545.1743931/94‑1 (MQ=60)
                                                                    TGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGT                         >  SRR4302545.1619148/1‑94 (MQ=60)
                                                                       ACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCA                      >  SRR4302545.3504437/1‑94 (MQ=60)
                                                                       ACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCA                      >  SRR4302545.7643572/1‑94 (MQ=60)
                                                                        CGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAG                     <  SRR4302545.4760408/94‑1 (MQ=60)
                                                                        CGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAG                     <  SRR4302545.2237040/94‑1 (MQ=60)
                                                                        CGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAG                     <  SRR4302545.5390585/94‑1 (MQ=60)
                                                                         GAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGG                    >  SRR4302545.7017202/1‑94 (MQ=60)
                                                                         GAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGG                    >  SRR4302545.5216267/1‑94 (MQ=60)
                                                                         GAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGG                    >  SRR4302545.9635190/1‑94 (MQ=60)
                                                                         GAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGG                    <  SRR4302545.3530693/94‑1 (MQ=60)
                                                                          AAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGA                   >  SRR4302545.10406763/1‑94 (MQ=60)
                                                                           AACCGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGAT                  <  SRR4302545.405610/94‑1 (MQ=60)
                                                                            AACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATC                 <  SRR4302545.7860791/94‑1 (MQ=60)
                                                                            AACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATC                 <  SRR4302545.4616239/94‑1 (MQ=60)
                                                                            AACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATC                 <  SRR4302545.320944/94‑1 (MQ=60)
                                                                             ACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCT                <  SRR4302545.9945606/94‑1 (MQ=60)
                                                                             ACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCT                >  SRR4302545.2633785/1‑94 (MQ=60)
                                                                             ACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCT                <  SRR4302545.4119504/94‑1 (MQ=60)
                                                                             ACGGCGTCCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCT                <  SRR4302545.1117828/94‑1 (MQ=60)
                                                                              CGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTG               <  SRR4302545.10142942/94‑1 (MQ=60)
                                                                              CGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTG               <  SRR4302545.1878501/94‑1 (MQ=60)
                                                                               GGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGA              >  SRR4302545.2101592/1‑94 (MQ=60)
                                                                               GGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGA              >  SRR4302545.5232532/1‑94 (MQ=60)
                                                                               GGCGTTACGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGA              >  SRR4302545.10835573/1‑94 (MQ=60)
                                                                                GCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGAT             <  SRR4302545.8160788/94‑1 (MQ=60)
                                                                                GCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGAT             <  SRR4302545.8549993/94‑1 (MQ=60)
                                                                                 CGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATG            <  SRR4302545.5574100/94‑1 (MQ=60)
                                                                                 CGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATG            <  SRR4302545.1839379/94‑1 (MQ=60)
                                                                                 CGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATG            >  SRR4302545.8901141/1‑94 (MQ=60)
                                                                                 CGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATG            <  SRR4302545.10157881/94‑1 (MQ=60)
                                                                                 CGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATG            <  SRR4302545.2635923/94‑1 (MQ=60)
                                                                                 CGTTCCGGCG‑AGCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATG            >  SRR4302545.1585274/1‑94 (MQ=60)
                                                                                  cataccggcgcGCGCAGCGTGGATCCAGTTTCTGATCATCATCCCGCTGATGATTATCCCGATGCTCGGTACCAATACCGTGCAGGATCTGATG            <  SRR4302545.395257/83‑1 (MQ=60)
                                                                                  GTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGA           >  SRR4302545.7908869/1‑94 (MQ=60)
                                                                                  GTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGA           >  SRR4302545.1276261/1‑94 (MQ=60)
                                                                                  GTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGA           >  SRR4302545.5759676/1‑94 (MQ=60)
                                                                                  GTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGA           <  SRR4302545.5967104/94‑1 (MQ=60)
                                                                                   TTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAA          <  SRR4302545.1058748/94‑1 (MQ=60)
                                                                                    TCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAAT         >  SRR4302545.10833715/1‑94 (MQ=60)
                                                                                      CGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATAC       >  SRR4302545.3056142/1‑94 (MQ=60)
                                                                                      CGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATAC       >  SRR4302545.1467027/1‑94 (MQ=60)
                                                                                      CGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATAC       <  SRR4302545.7551772/94‑1 (MQ=60)
                                                                                       GGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACT      <  SRR4302545.1416370/94‑1 (MQ=60)
                                                                                       GGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACT      <  SRR4302545.1201397/94‑1 (MQ=60)
                                                                                        GCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTA     <  SRR4302545.4423925/94‑1 (MQ=60)
                                                                                           gcGCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATTATATT   >  SRR4302545.7067984/3‑94 (MQ=60)
                                                                                           gcGCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATT   <  SRR4302545.8237738/92‑1 (MQ=60)
                                                                                           gcGCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATT   <  SRR4302545.754387/92‑1 (MQ=60)
                                                                                           gcGCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATT   <  SRR4302545.6252803/92‑1 (MQ=60)
                                                                                           gcGCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATT   <  SRR4302545.4269449/92‑1 (MQ=60)
                                                                                            CGCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTA  <  SRR4302545.1108802/94‑1 (MQ=60)
                                                                                            CGCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTA  >  SRR4302545.1895163/1‑94 (MQ=60)
                                                                                            CGCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTA  >  SRR4302545.8642330/1‑94 (MQ=60)
                                                                                             |                                                                                            
CGCAACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGCGGCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTAT‑CCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTA  >  NC_000913/3224786‑3224970

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑