Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 647 270.1 21258240 98.4% 20918108 94.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,224,926 +C coding (1052/1434 nt) ygjI → putative oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,224,9241.C99.6% 1730.1 / ‑2.6 495coding (1050/1434 nt)ygjIputative oxidoreductase
Reads supporting (aligned to +/- strand):  ref base . (1/1);  new base C (241/252);  total (242/253)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 541 total aligned reads displayed.

TCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGCGGCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTAT‑CCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCTTCCGCCGTTATTCA  >  NC_000913/3224830‑3225012
                                                                                               |                                                                                        
 cTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTAT‑c                                                                                         >  1:5526485/1‑94 (MQ=255)
  tttGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTAT‑cc                                                                                        <  1:9210033/94‑1 (MQ=255)
  tttGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTAT‑cc                                                                                        <  2:10019920/94‑1 (MQ=255)
   ttGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTAT‑ccc                                                                                       >  2:3385085/1‑93 (MQ=255)
   ttGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTAT‑ccc                                                                                       >  1:3251062/1‑93 (MQ=255)
     ggTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTAT‑cccga                                                                                     >  1:8809314/1‑91 (MQ=255)
        aaGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGc                                                                                   >  1:6593595/1‑94 (MQ=255)
        aaGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGc                                                                                   >  1:4194942/1‑94 (MQ=255)
         aGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCt                                                                                  >  1:9129679/1‑94 (MQ=255)
         aGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCt                                                                                  >  1:2317378/1‑94 (MQ=255)
          aaaaaCCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTGCTGATCGTCATCCCGCTGATGATTATCCCGATGCTc                                                                                 <  1:3130402/93‑1 (MQ=255)
            aaaCCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCgg                                                                               >  1:3723603/1‑94 (MQ=255)
             aaCCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGt                                                                              >  1:4322157/1‑94 (MQ=255)
              aCCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGtt                                                                             <  1:1249254/94‑1 (MQ=255)
              aCCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGtt                                                                             >  2:329686/1‑94 (MQ=255)
               ccGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTc                                                                            <  1:10057244/94‑1 (MQ=255)
                 gTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCa                                                                          >  1:9607687/1‑94 (MQ=255)
                 gTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCa                                                                          <  2:4718922/94‑1 (MQ=255)
                 gTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCa                                                                          >  1:5708334/1‑94 (MQ=255)
                  tCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCaa                                                                         <  1:7695397/94‑1 (MQ=255)
                   cGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGCTGATTATCCCTATGCTCGGTTCCAAt                                                                        >  2:8875615/1‑94 (MQ=255)
                   cGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAAt                                                                        >  1:2780243/1‑94 (MQ=255)
                   cGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAAt                                                                        <  1:717716/94‑1 (MQ=255)
                   cGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAAt                                                                        <  1:782777/94‑1 (MQ=255)
                    gAACTGAACGAAAACGGCGTCCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATa                                                                       <  2:6837852/94‑1 (MQ=255)
                       cTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCg                                                                    >  1:3582558/1‑94 (MQ=255)
                        tGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGt                                                                   >  2:8210968/1‑94 (MQ=255)
                         gAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTg                                                                  <  2:8686622/94‑1 (MQ=255)
                             gAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAgg                                                              >  1:6829540/1‑94 (MQ=255)
                             gAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAgg                                                              <  2:6174166/94‑1 (MQ=255)
                             gAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAgg                                                              >  1:9360612/1‑94 (MQ=255)
                               aaaCGGCGTCCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGAt                                                            <  2:10215197/94‑1 (MQ=255)
                                aaCGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATc                                                           >  2:4119480/1‑94 (MQ=255)
                                 aCGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCt                                                          >  2:9747490/1‑94 (MQ=255)
                                  cGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCtg                                                         <  1:9850221/94‑1 (MQ=255)
                                   ggCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCtga                                                        >  1:3950704/1‑94 (MQ=255)
                                    gCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCtgat                                                       <  1:7935366/94‑1 (MQ=255)
                                    gCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCtgat                                                       <  1:8309209/94‑1 (MQ=255)
                                     cGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCtgatg                                                      <  1:2573928/94‑1 (MQ=255)
                                     cGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCtgatg                                                      >  2:9071348/1‑94 (MQ=255)
                                        tCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAAt                                                   <  2:7084368/94‑1 (MQ=255)
                                         ccGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATa                                                  <  2:1545493/94‑1 (MQ=255)
                                          cggcgc‑gcgcAGCGTGGATCCAGTTCCTGATGGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATAc                                                 <  2:3025712/94‑1 (MQ=255)
                                          cggcgc‑gcgcAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATAc                                                 >  2:8621133/1‑94 (MQ=255)
                                               gcgcgcAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACtatt                                             >  1:1570621/3‑94 (MQ=255)
                                               gcgcgcAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACtatt                                             <  1:737800/92‑1 (MQ=255)
                                               gcgcgcAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACtatt                                             <  2:737365/92‑1 (MQ=255)
                                                cgcgcAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACtatta                                            >  1:10500310/2‑94 (MQ=255)
                                                cgcgcAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACtatta                                            <  1:1085287/93‑1 (MQ=255)
                                                 gcgcAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACtattat                                           <  2:2617753/94‑1 (MQ=255)
                                                 gcgcAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACtattat                                           <  2:1089725/94‑1 (MQ=255)
                                                  cgcAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTattatt                                          >  1:8715513/1‑94 (MQ=255)
                                                  cgcAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTattatt                                          >  2:1788995/1‑94 (MQ=255)
                                                   gcAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTAttatta                                         >  2:5141714/1‑94 (MQ=255)
                                                     aGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAat                                       <  1:4139819/94‑1 (MQ=255)
                                                     aGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAat                                       <  2:1971034/94‑1 (MQ=255)
                                                     aGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGAAGGATCTGATGAATACTATTATTAat                                       >  2:6459007/1‑94 (MQ=255)
                                                     aGCATGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAat                                       <  2:2364301/94‑1 (MQ=255)
                                                      gCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAata                                      >  2:10396519/1‑94 (MQ=255)
                                                      gCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAata                                      >  2:7669660/1‑94 (MQ=255)
                                                      gCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAata                                      >  2:3161442/1‑94 (MQ=255)
                                                       cGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAatat                                     <  1:8440327/94‑1 (MQ=255)
                                                       cGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAatat                                     <  2:2862670/94‑1 (MQ=255)
                                                        gTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATg                                    >  2:5699717/1‑94 (MQ=255)
                                                        gTGGATCAACTTCCTGATCGTCATCCCGCTGATGATGATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATg                                    <  1:4549693/94‑1 (MQ=255)
                                                         tGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGa                                   <  1:6223812/94‑1 (MQ=255)
                                                         tGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGa                                   <  2:2257489/94‑1 (MQ=255)
                                                         tGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGa                                   <  2:1324477/94‑1 (MQ=255)
                                                          ggATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGAc                                  >  1:1448522/1‑94 (MQ=255)
                                                          ggATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGAc                                  >  1:1864217/1‑94 (MQ=255)
                                                          ggATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGAc                                  <  2:1417727/94‑1 (MQ=255)
                                                           gATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGAcc                                 <  2:6596084/94‑1 (MQ=255)
                                                           gATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGAcc                                 <  2:2134131/94‑1 (MQ=255)
                                                            aTCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGAccg                                <  2:3913588/94‑1 (MQ=255)
                                                            aTCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGAccg                                <  1:1129449/94‑1 (MQ=255)
                                                             tCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGAccgc                               <  2:477158/94‑1 (MQ=255)
                                                              ccAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGAccgcc                              >  1:4004870/1‑94 (MQ=255)
                                                                  ttCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAg                          <  1:2950002/94‑1 (MQ=255)
                                                                   tCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGc                         <  2:7258403/94‑1 (MQ=255)
                                                                   tCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGc                         <  2:5295242/94‑1 (MQ=255)
                                                                     cTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGt                       >  2:5415985/1‑94 (MQ=255)
                                                                       gATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTcc                     <  2:9457486/94‑1 (MQ=255)
                                                                         tCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCAt                   <  1:10296951/94‑1 (MQ=255)
                                                                         tCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCAt                   <  2:5541340/94‑1 (MQ=255)
                                                                           gTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGc                 >  2:4394282/1‑94 (MQ=255)
                                                                            tCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCt                >  2:7560363/1‑94 (MQ=255)
                                                                             cATCGCGCTGATGATTATCCCGATGCTCTGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCtt               <  1:6723635/94‑1 (MQ=255)
                                                                             cATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCtt               >  2:588398/1‑94 (MQ=255)
                                                                             cATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCtt               >  2:9257068/1‑94 (MQ=255)
                                                                             cATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCtt               >  1:9966433/1‑94 (MQ=255)
                                                                              ctCCCACTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATTCTATTATTAATATGACCGCCGCAGCGTCCATGCTTc              <  1:2138246/93‑1 (MQ=255)
                                                                                 ccGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGAACGGCGCAGCGTCCATGCTTccgc           >  1:1752989/1‑94 (MQ=255)
                                                                                  cGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCTTccgcc          >  1:5397691/1‑94 (MQ=255)
                                                                                  cGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCTTccgcc          >  2:6819997/1‑94 (MQ=255)
                                                                                    cTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCTTCCGCCGt        >  2:3616091/1‑94 (MQ=255)
                                                                                     tgatgaTTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCTTCCGCCGtt       >  2:8464597/1‑94 (MQ=255)
                                                                                      gatgatTATCCCGATGCTCGGTTACAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCTTCCGCCGTTa      <  1:5904013/94‑1 (MQ=255)
                                                                                       atgatTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCTTCCGCCGTTAt     <  2:837395/94‑1 (MQ=255)
                                                                                       atgatTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGCCCATGCTTCCGCCGTTAt     >  1:2372084/1‑94 (MQ=255)
                                                                                        tgatTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCTTCCGCCGTTAtt    >  1:4913361/1‑94 (MQ=255)
                                                                                               |                                                                                        
TCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGCGGCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTAT‑CCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCTTCCGCCGTTATTCA  >  NC_000913/3224830‑3225012

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 326 total aligned reads displayed.
CAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGGACGTCTTCCGACGTTGGCCGTTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTG  >  NC_000913/3236086‑3236270
                                                                                           |                                                                                             
 AACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCT‑GACGT                                                                                           <  SRR4302545.8274842/94‑1 (MQ=60)
 AACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCT‑GACGT                                                                                           <  SRR4302545.5049945/94‑1 (MQ=60)
 AACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCT‑GACGT                                                                                           >  SRR4302545.1605880/1‑94 (MQ=60)
     CGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATCAAAAGCAGCGGCT‑GACGTCTTC                                                                                       >  SRR4302545.10154501/1‑94 (MQ=60)
     CGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCT‑GACGTCTTC                                                                                       >  SRR4302545.814569/1‑94 (MQ=60)
     CGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCT‑GACGTCTTC                                                                                       <  SRR4302545.10193406/94‑1 (MQ=60)
     CGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCT‑GACGTCTTC                                                                                       <  SRR4302545.3863651/94‑1 (MQ=60)
     CGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTACGCATAAAAAGCAGCGGCT‑GACGTCTTC                                                                                       <  SRR4302545.10913969/94‑1 (MQ=60)
      GAAAGGAATACGCATACGTATTCCTTTTTTGTTGTAATTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCT‑GACGTCTTCC                                                                                      <  SRR4302545.4046257/94‑1 (MQ=60)
      GAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCT‑GACGTCTTCC                                                                                      >  SRR4302545.9509524/1‑94 (MQ=60)
      GAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCT‑GACGTCTTCC                                                                                      <  SRR4302545.2686855/94‑1 (MQ=60)
       AAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCT‑GACGTCTTCCG                                                                                     <  SRR4302545.9227298/94‑1 (MQ=60)
       AAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCT‑GACGTCTTCCG                                                                                     <  SRR4302545.6658841/94‑1 (MQ=60)
       AAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCT‑GACGTCTTCCA                                                                                     <  SRR4302545.1806424/94‑1 (MQ=60)
        AATGAATACGCAGACGAATTCCTTTTTTGTTGTAAGTGTGACCGTGCCGGAAGGCGAGGTCTATGCATAAAAAGTAGCGGCT‑GACCTCTTCCGA                                                                                    >  SRR4302545.6943871/1‑94 (MQ=60)
        AAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCT‑GACGTCTTCGGA                                                                                    >  SRR4302545.5177036/1‑94 (MQ=60)
        AAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCT‑GACGTCTTCCGA                                                                                    >  SRR4302545.10491331/1‑94 (MQ=60)
        AAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCT‑GACGTCTTCCGA                                                                                    >  SRR4302545.2168038/1‑94 (MQ=60)
        AAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCT‑GACGTCTTCCGA                                                                                    >  SRR4302545.9905569/1‑94 (MQ=60)
        AAGGAATACGCAGACGGATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGG‑CGTCTTCCGA                                                                                    >  SRR4302545.4242779/1‑94 (MQ=60)
         AGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCT‑GACGTCTTCCGAC                                                                                   >  SRR4302545.7091877/1‑94 (MQ=60)
          GGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCT‑GACGTCTTCCGACG                                                                                  >  SRR4302545.5411518/1‑94 (MQ=60)
          GGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCT‑GACGTCTTCCGACG                                                                                  <  SRR4302545.416162/94‑1 (MQ=60)
          GGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCT‑GACGTCTTCCGACG                                                                                  <  SRR4302545.10881417/94‑1 (MQ=60)
          GGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCT‑GACGTCTTCCGACG                                                                                  <  SRR4302545.8781555/94‑1 (MQ=60)
           GAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCT‑GACGTCTTCCGACGT                                                                                 >  SRR4302545.6133779/1‑94 (MQ=60)
            AATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCT‑GACGTCTTCCGACGTT                                                                                >  SRR4302545.4987413/1‑94 (MQ=60)
            AATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCT‑GACGTCTTCCGACGTT                                                                                <  SRR4302545.7094623/94‑1 (MQ=60)
            AATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCT‑GACGTCTTCCGACGTT                                                                                >  SRR4302545.5583964/1‑94 (MQ=60)
            AATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCT‑GACGTCTTCCGACGTT                                                                                <  SRR4302545.6962194/94‑1 (MQ=60)
            AATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCT‑GACGTCTTCCGACGTT                                                                                <  SRR4302545.6009966/94‑1 (MQ=60)
             ATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCT‑GACGTCTTCCGACGTTG                                                                               >  SRR4302545.4470026/1‑94 (MQ=60)
             ATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCT‑GACGTCTTCCGACGTTG                                                                               <  SRR4302545.8387697/94‑1 (MQ=60)
             ATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCT‑GACGTCTTCCGACGTTG                                                                               <  SRR4302545.2304850/94‑1 (MQ=60)
             ATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCT‑GACGTCTTCCGACGGTG                                                                               >  SRR4302545.9195757/1‑94 (MQ=60)
              TACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCT‑GACGTCTTCCGACGTTGG                                                                              >  SRR4302545.8818045/1‑94 (MQ=60)
              TACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCT‑GACGTCTTCCGACGTTGG                                                                              >  SRR4302545.2528706/1‑94 (MQ=60)
              TACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCT‑GACGTCTTCCGACGTTGG                                                                              <  SRR4302545.1283202/94‑1 (MQ=60)
              TACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCT‑GACGTCTTCCGACGTTGG                                                                              <  SRR4302545.8087156/94‑1 (MQ=60)
               ACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCT‑GACGTCTTCCGACGTTGGC                                                                             >  SRR4302545.4541152/1‑94 (MQ=60)
                CGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAACAGCAGCGGCT‑GACGTCTTCCGACGGTGGCC                                                                            >  SRR4302545.9800862/1‑94 (MQ=60)
                CGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCT‑GACGTCTTCCGACGTTGGCC                                                                            >  SRR4302545.8620444/1‑94 (MQ=60)
                CGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCT‑GACGTCTTCCGACGTTGGCC                                                                            <  SRR4302545.8488352/94‑1 (MQ=60)
                CGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCT‑GACGTCTTCCGACGTTGGCC                                                                            >  SRR4302545.1717503/1‑94 (MQ=60)
                 GCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCT‑GACGTCTTCCGACGTTcgacg                                                                           >  SRR4302545.8267497/1‑89 (MQ=60)
                 GCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCT‑GACGTCTTCCGACGTTGGCCG                                                                           <  SRR4302545.8887593/94‑1 (MQ=60)
                 GCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCT‑GACGTCTTCCGACGTTGGCCG                                                                           >  SRR4302545.8223374/1‑94 (MQ=60)
                  CAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCT‑GACGTCCTCCGACGTTGGCCGT                                                                          >  SRR4302545.9793496/1‑94 (MQ=60)
                    GACGTATTCTTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCT‑GACGTCTTCCGACGTTGGCCGTTT                                                                        <  SRR4302545.8745582/94‑1 (MQ=60)
                     ACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCT‑GACGTCTTCCGACGTTGGCCGTTTT                                                                       <  SRR4302545.4231654/94‑1 (MQ=60)
                     ACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCT‑GACGTCTTCCGACGTTGGCCGTTTT                                                                       >  SRR4302545.6514105/1‑94 (MQ=60)
                     ACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCT‑GACGTCTTCCGACGTTGGCCGTTTT                                                                       >  SRR4302545.10629623/1‑94 (MQ=60)
                      CGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCT‑GACGTCTTCCGACGTTGGCCGTTTTT                                                                      <  SRR4302545.780914/94‑1 (MQ=60)
                      CGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCT‑GACGTCTTCCGACGTTGGCCGTTTTT                                                                      >  SRR4302545.8580230/1‑94 (MQ=60)
                       GTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCT‑GACGTCTTCCGACGTTGGCCGTTTTTT                                                                     <  SRR4302545.697584/94‑1 (MQ=60)
                       GTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCT‑GACGTCTTCCGACGTTGGCCGTTTTTT                                                                     >  SRR4302545.3751619/1‑94 (MQ=60)
                       GTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCT‑GACGTCTTCCGACGTTGGCCGTTTTTT                                                                     >  SRR4302545.4612262/1‑94 (MQ=60)
                       GTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCT‑GACGTCTTCCGACGTTGGCCGTTTTTT                                                                     >  SRR4302545.4612263/1‑94 (MQ=60)
                        TATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCT‑GACGTCTTCCGACGTTGGCCGTTTTTTT                                                                    <  SRR4302545.7674818/94‑1 (MQ=60)
                        TATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCT‑GACGTCTTCCGACGTTGGCCGTTTTTTT                                                                    <  SRR4302545.2085977/94‑1 (MQ=60)
                        TATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCT‑GACGTCTTCCGACGTTGGCCGTTTTTTT                                                                    >  SRR4302545.2403447/1‑94 (MQ=60)
                         ATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCCGCGGCT‑GACGTCTTCAGACGTTGGttgtttttgtt                                                                   >  SRR4302545.9039442/1‑83 (MQ=60)
                         ATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCT‑GACGTCTTCCGACGTTGGCCGTTTTTTTA                                                                   <  SRR4302545.2294186/94‑1 (MQ=60)
                          TTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCT‑GACGTCTTCCGACGTTGGCCGTTTTTTTAT                                                                  <  SRR4302545.10545137/94‑1 (MQ=60)
                           TCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCT‑GACGTCTTCCGACGTTGGCCGTTTTTTTATG                                                                 >  SRR4302545.9093697/1‑94 (MQ=60)
                           TCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCT‑GACGTCTTCCGACGTTGGCCGTTTTTTTATG                                                                 <  SRR4302545.275660/94‑1 (MQ=60)
                            CCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCT‑GACGTCTTCCGACGTTGGCCGTTTTTTTATGT                                                                >  SRR4302545.4828092/1‑94 (MQ=60)
                            CCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCT‑GACGTCTTCCGACGTTGGCCGTTTTTTTATGT                                                                <  SRR4302545.6744475/94‑1 (MQ=60)
                            CCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCT‑GACGTCTTCCGACGTTGGCCGTTTTTTTATGT                                                                >  SRR4302545.2347425/1‑94 (MQ=60)
                             CTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAATCAGCGGCT‑GACGTCTTCCGACGTTGGCCGTTTTTTTATGTG                                                               <  SRR4302545.5568752/94‑1 (MQ=60)
                             CTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCT‑GACGTCTTCCGACGTTGGCCGTTTTTTTATGTG                                                               >  SRR4302545.1270268/1‑94 (MQ=60)
                             CTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCT‑GACGTCTTCCGACGTTGGCCGTTTTTTTATGTG                                                               <  SRR4302545.7185041/94‑1 (MQ=60)
                             CTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCT‑GACGTCTTCCGACGTTGGCCGTTTTTTTATGTG                                                               >  SRR4302545.4542607/1‑94 (MQ=60)
                              TTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCT‑GACGTCTTCCGACGTTGGCCGTTTTTTTATGTGT                                                              <  SRR4302545.2462280/94‑1 (MQ=60)
                              TTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCT‑GACGTCTTCCGACGTTGGCCGTTTTTTTATGTGT                                                              <  SRR4302545.2462279/94‑1 (MQ=60)
                              TTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCT‑GACGTCTTCCGACGTTGGCCGTTTTTTTATGTGT                                                              >  SRR4302545.9793859/1‑94 (MQ=60)
                              TTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCT‑GACGTCTTCCGACGTTGGCCGTTTTTTTATGTGT                                                              >  SRR4302545.6787806/1‑94 (MQ=60)
                                 TTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCT‑GACGTCTTCCGACGTTGGCCGTTTTTTTATGTGTAAG                                                           <  SRR4302545.10319694/94‑1 (MQ=60)
                                 TTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCT‑GACGTCTTCCGACGTTGGCCGTTTTTTTATGTGTAAG                                                           >  SRR4302545.8037284/1‑94 (MQ=60)
                                  TTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCT‑GACGTCTTCCGACGTTGGCCGTTTTTTTATGTGTAAGG                                                          <  SRR4302545.48754/94‑1 (MQ=60)
                                  TTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCT‑GACGTCTTCCGACGTTGGCCGTTTTTTTATGTGTAAGG                                                          >  SRR4302545.8074662/1‑94 (MQ=60)
                                  TTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCT‑GACGTCTTCCGACGTTGGCCGTTTTTTGATGTGTAAGG                                                          <  SRR4302545.2540448/94‑1 (MQ=60)
                                   TGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCT‑GACGTCTTCCGACGTTGGCCGTTTTTTTATGTGTAAGGA                                                         >  SRR4302545.6454289/1‑94 (MQ=60)
                                   TGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCT‑GACGTCTTCCGACGTTGGCCGTTTTTTTATGTGTAAGGA                                                         <  SRR4302545.340313/94‑1 (MQ=60)
                                   TGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCT‑GACGTCTTCCGACGTTGGCCGTTTTTTTATGTGTAAGGA                                                         >  SRR4302545.3400165/1‑94 (MQ=60)
                                    GTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCT‑GACGTCTTCCGACGTTGGCCGTTTTTTTATGTGTAAGGAA                                                        <  SRR4302545.3514524/94‑1 (MQ=60)
                                    GTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCT‑GACGTCTTCCGACGTTGGCCGTTTTTTTATGTGTAAGGAA                                                        >  SRR4302545.2783086/1‑94 (MQ=60)
                                     TTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCT‑GACGTCTTCCGACGTTGGCCGTTTTTTTATGTGTAAGGAAC                                                       <  SRR4302545.102345/94‑1 (MQ=60)
                                     TTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCT‑GACGTCTTCCGACGTTGGCCGTTTTTTTATGTGTAAGGAAC                                                       >  SRR4302545.10716540/1‑94 (MQ=60)
                                     TTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCT‑GACGTCTTCCGACGTTGGCCGTTTTTTTATGTGTAAGGAAC                                                       >  SRR4302545.5453282/1‑94 (MQ=60)
                                       GTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCT‑GACGTCTTCCGACGTTGGCCGTTTTTTTATGTGTAAGGAACTT                                                     <  SRR4302545.3769990/94‑1 (MQ=60)
                                        TAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCT‑GACGTCTTCCGACGTTGGCCGTTTTTTTATGTGTAAGGAACTTC                                                    >  SRR4302545.9222298/1‑94 (MQ=60)
                                         AAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCT‑GACGTCTTCCGACGTTGGCCGTTTTTTTATGTGTAAGGAACTTCT                                                   <  SRR4302545.1570411/94‑1 (MQ=60)
                                         AAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCT‑GACGTCTTCCGACGTTGGCCGTTTTTTTATGTGTAAGGAACTTCT                                                   <  SRR4302545.2196033/94‑1 (MQ=60)
                                          AGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCT‑GACGTCTTCCGACGTTGGCCGTTTTTTTATGTGTAAGGAACTTCTA                                                  >  SRR4302545.7652140/1‑94 (MQ=60)
                                          AGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCT‑GACGTCTTCCGACGTTGGCCGTTTTTTTATGTGTAAGGAACTTCTA                                                  >  SRR4302545.4714338/1‑94 (MQ=60)
                                          AGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCT‑GACGTCTTCCGACGTTGGCCGTTTTTTTATGTGTAAGGAACTTCTA                                                  <  SRR4302545.8349754/94‑1 (MQ=60)
                                          AGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCACCGGCT‑GACGTCTTTCGACGTTGGTCGTTTTTTTTTGTGTgagctcattcta                                                  >  SRR4302545.9863300/1‑82 (MQ=60)
                                          AGGGAGACCTTGGCGGAAGGCGAGGTCTATGTATAAAAAGCAGCGGCT‑GACGTCTTCCGACGTTGGCCGTTTTTTTATGTGTAAGGAACATCTA                                                  <  SRR4302545.8350040/94‑1 (MQ=60)
                                           GTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCT‑GACGTCTTCCGACGTTGGCCGTTTTTTTATGTGTAAGGAACTTCTAT                                                 >  SRR4302545.8885505/1‑94 (MQ=60)
                                           GTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCT‑GACGTCTTCCGACGTTGGCCGTTTTTTTATGTGTAAGGAACTTCTAT                                                 <  SRR4302545.6941411/94‑1 (MQ=60)
                                           GTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCT‑GACGTCTTCCGACGTTGGCCGTTTTTTTATGTGTAAGGAACTTCTAT                                                 >  SRR4302545.9124789/1‑94 (MQ=60)
                                           GTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCT‑GACGTCTTCCGACGTTGGCCGTTTTTTTATGTGTAAGGAACTTCTAT                                                 <  SRR4302545.3329906/94‑1 (MQ=60)
                                            TGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCT‑GACGTCTTCCGACGTTGGCCGTTTTTTTATGTGTAAGGAACTTCTATG                                                >  SRR4302545.506607/1‑94 (MQ=60)
                                             GAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCT‑GACGTCTTCCGACGTTGGCCGTTTTTTTATGTGTAAGGAACTTCTATGA                                               >  SRR4302545.3537286/1‑94 (MQ=60)
                                             GAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCT‑GACGTCTTCCGACGTTGGCCGTTTTTTTATGTGTAAGGAACTTCTATGA                                               <  SRR4302545.2027566/94‑1 (MQ=60)
                                             GAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCT‑GACGTCTTCCGACGTTGGCCGTTTTTTTATGTGTAAGGAACTTCTATGA                                               <  SRR4302545.8983213/94‑1 (MQ=60)
                                              AGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCT‑GACGTCTTCCGACGTTGGCCGTTTTTTTATGTGTAAGGAACTTCTATGAA                                              <  SRR4302545.3264835/94‑1 (MQ=60)
                                              AGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCT‑GACGTCTTCCGACGTTGGCCGTTTTTTTATGTGTAAGGAACTTCTATGAA                                              <  SRR4302545.6550234/94‑1 (MQ=60)
                                              AGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCT‑GACGTCTTCCGACGTTGGCCGTTTTTTTATGTGTAAGGAACTTCTATGAA                                              <  SRR4302545.9660963/94‑1 (MQ=60)
                                               GCCCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCT‑GACGTCTTCCGACGTTGGCCGTTTTTTTATGTGTAAGGAACTTCTATGAAT                                             <  SRR4302545.2546010/94‑1 (MQ=60)
                                               GACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCT‑GACGTCTTCCGACGTTGGCCGTTTTTTTATGTGTAAGGAACTTCTATGAAT                                             <  SRR4302545.2624650/94‑1 (MQ=60)
                                               GACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCT‑GACGTCTTCCGACGTTGGCCGTTTTTTTATGTGTAAGGAACTTCTATGAAT                                             >  SRR4302545.2754625/1‑94 (MQ=60)
                                               GACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCT‑GACGTCTTCCGACGTTGGCCGTTTTTTTATGTGTAAGGAACTTCTATGAAT                                             <  SRR4302545.1084893/94‑1 (MQ=60)
                                                ACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCT‑GACGTCTTCCGACGTTGGCCGTTTTTTTATGTGTAAGGCACTTCTATGAATA                                            >  SRR4302545.1931595/1‑94 (MQ=60)
                                                ACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCT‑GACGTCTTCCGACGTTGGCCGTTTTTTTATGTGTAAGGAACTTCTATGAATA                                            <  SRR4302545.10619900/94‑1 (MQ=60)
                                                ACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCT‑GACGTCTTCCGACGTTGGCCGTTTTTTTATGTGTAAGGAACTTCTATGAATA                                            >  SRR4302545.1931594/1‑94 (MQ=60)
                                                ACCTTACCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCT‑GACGTCTTCCGACGTTGGCCGTTTTTTTATGTGTAAGGAACTTCTATGAATA                                            <  SRR4302545.3832442/94‑1 (MQ=60)
                                                 CCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCT‑GACGTCTTCCGACGTTGGCCGTTTTTTTATGTGTAAGGAACTTCTATGAATAC                                           >  SRR4302545.3089462/1‑94 (MQ=60)
                                                  CTTGCCGGAAGGCGAGGTCTATGCATATAAAGCAGCGGCT‑GACGTCGTCCGACGTTGGCCGTTTTTTTATGTGTAAGGAACTTCTATTAATTCT                                          >  SRR4302545.9685537/1‑94 (MQ=60)
                                                  CTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCT‑GACGTCTTCCGACGTTGGCCGTTTTTTTATGTGTAAGGAACTTCTATGAATACT                                          <  SRR4302545.8314652/94‑1 (MQ=60)
                                                  CTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCT‑GACGTCTTCCGACGTTGGCCGTTTTTTTATGTGTAAGGAACTTCTATGAATACT                                          <  SRR4302545.10928169/94‑1 (MQ=60)
                                                  CTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCT‑GACGTCTTCCGACGTTGGCCGTTTTTTTATGTGTAAGGAACTTCTATGAATACT                                          >  SRR4302545.255361/1‑94 (MQ=60)
                                                   TTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCT‑GACGTCTTCCGACGTTGGCCGTTTTTTTATGTGTAAGGAACTTCTATGAATACTG                                         <  SRR4302545.4311491/94‑1 (MQ=60)
                                                   TTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCT‑GACGTCTTCCGACGTTGGCCGTTTTTTTATGTGTAAGGAACTTCTATGAATACTG                                         >  SRR4302545.4764744/1‑94 (MQ=60)
                                                   TTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCT‑GACGTCTTCCGACGTTGGCCGTTTTTTTATGTGTAAGGAACTTCTATGAATACTG                                         <  SRR4302545.1188479/94‑1 (MQ=60)
                                                    TGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCT‑GACGTCTTCCGACGTTGGCCGTTTTTTTATGTGTAAGGAACTTCTATGAATACTGT                                        >  SRR4302545.6271462/1‑94 (MQ=60)
                                                     GCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCT‑GACGTCTTCCGACGTTGGCCGTTTTTTTATGTGTAAGGAACTTCTATGAATACTGTC                                       <  SRR4302545.3961306/94‑1 (MQ=60)
                                                     GCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCT‑GACGTCTTCCGACGTTGACCGTTTTTTTATGTGTAAGGAACTTCTATGAATACTGTC                                       <  SRR4302545.9280083/94‑1 (MQ=60)
                                                      GCGGAAGGCGAGGTGTATGCATAAAAAGCAGCGGCT‑GACGTCTTCCGACGTTGGCCGTTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCG                                      <  SRR4302545.9291777/94‑1 (MQ=60)
                                                      CCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCT‑GACGTCTTCCGACGTTGGCCGTTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCG                                      >  SRR4302545.1010605/1‑94 (MQ=60)
                                                      CCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCT‑GACGTCTTCCGACGTTGGCCGTTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCG                                      >  SRR4302545.7664569/1‑94 (MQ=60)
                                                       GGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCT‑GACGTCTTCCGACGTTGGCCGTTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGG                                     <  SRR4302545.7767873/94‑1 (MQ=60)
                                                       CGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCT‑GACGTCTTCCGACGTTGGCCGTTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGG                                     >  SRR4302545.3596869/1‑94 (MQ=60)
                                                       CGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCT‑GACGTCTTCCGACGTTGGCCGTTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGG                                     <  SRR4302545.7740580/94‑1 (MQ=60)
                                                       CGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCT‑GACGTCTTCCGACGTTGGCCGTTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGC                                     >  SRR4302545.7894346/1‑94 (MQ=60)
                                                        GGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGG‑CGTCTTCCGACGTTGGCCGGTTTTTTATGTGTGAGGAACTTCTATGAATACTGTCGGC                                    >  SRR4302545.10305082/1‑94 (MQ=60)
                                                        GGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCT‑GACGTCTTCCGACGTTGGCCGTTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGC                                    <  SRR4302545.5251689/94‑1 (MQ=60)
                                                          AAGGCGAGGTCTATGCATAAAAAGCAGCGGCT‑GACGTCTTCCGACGTTGGCCGTTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCAC                                  <  SRR4302545.9760385/94‑1 (MQ=60)
                                                           AGGCGAGGTCTATGCATAAAAAGCAGCGGCT‑GACGTCTTCCGACGTTGGCCGTTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACG                                 >  SRR4302545.10500219/1‑94 (MQ=60)
                                                           AGGCGAGGTCTATGCATAAAAAGCAGCGGCT‑GACGTCTTCCGACGTTGGCCGTTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACG                                 <  SRR4302545.23925/94‑1 (MQ=60)
                                                           AGGCGAGGTCTATGCATAAAAAGCAGCGGCT‑GACGTCTTCCGACGTTGGCCGTTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACG                                 >  SRR4302545.2622506/1‑94 (MQ=60)
                                                           AGGCGAGGTCTATGCATAAAAAGCAGCGGCT‑GACGTCTTCCGACGTTGGCCGTTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACG                                 <  SRR4302545.7638295/94‑1 (MQ=60)
                                                            GGCGAGGTCTATGCATAAAAAGCAGCGGCT‑GACGTCTTCCGACGTTGGCCGTTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGC                                >  SRR4302545.8401969/1‑94 (MQ=60)
                                                             GCGAGGTCTATGCATAAAAAGCAGCGGCT‑GACGTCTTCCGACGTTGGCCGTTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCC                               <  SRR4302545.7415289/94‑1 (MQ=60)
                                                             GCGAGGTCTATGCATAAAAAGCAGCGGCT‑GACGTCTTCCGACGTTGGCCGTTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCC                               <  SRR4302545.935557/94‑1 (MQ=60)
                                                              TGAGGTCTATGCATAAAAAGCAGCGGCT‑GACGTCTTCCGACGTTGGCCGTTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCG                              >  SRR4302545.7800629/1‑94 (MQ=60)
                                                               GAGGTCTATGCATAAAAAGCAGCGGCT‑GACGTCTTCCGACGTTGGCCGTTTTTTTATGTGTAAGGAACTTCTCTGAATACTGTCGGCACGCCGT                             >  SRR4302545.3627272/1‑94 (MQ=60)
                                                               GAGGTCTATGCATAAAAAGCAGCGGCT‑GACGTCTTCCGACGTTGGCCGTTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGT                             >  SRR4302545.1127997/1‑94 (MQ=60)
                                                                AGGTCTATGCATAAAAAGCAGCGGCT‑GACGTCTTCCGACGTTGGCCGTTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTT                            <  SRR4302545.9504808/94‑1 (MQ=60)
                                                                AGGTCTATGCATAAAAAGCAGCGGCT‑GACGTCTTCCGACGTTGGCCGTTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTT                            >  SRR4302545.9469107/1‑94 (MQ=60)
                                                                AGGTCTATGCATAAAAAGCAGCGGCT‑GACGTCTTCCGACGTTGGCCGTTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTT                            >  SRR4302545.10337994/1‑94 (MQ=60)
                                                                AGGTCTATGCATAAAAAGCAGCGGCT‑GACGTCTTCCGACGTTGGCCGTTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTT                            >  SRR4302545.4364081/1‑94 (MQ=60)
                                                                AGGTCTATGCATAAAAAGCAGCGGCT‑GACGTCTTCCGACGTTGGCCGTTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTT                            <  SRR4302545.4759402/94‑1 (MQ=60)
                                                                AGGTCTATGCATAAAAAGCAGCGGCT‑GACGTCTTCCGACGTTGGCCGTTTTTTTATGTGTAAGGAACTTCGATGAATACTGTCGGCACGCCGTT                            >  SRR4302545.6005595/1‑94 (MQ=60)
                                                                AGGTCTATGCATAAAAAGCAGCGGCT‑GACGTCGTCCGACGTTGGCCGTTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTT                            <  SRR4302545.2884359/94‑1 (MQ=60)
                                                                 GGTCTATGCATAAAAAGCAGCGGCT‑GACGTCTTCCGACGTTGGCCGTTTTTTTATGTTTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTG                           >  SRR4302545.3710039/1‑94 (MQ=60)
                                                                 GGTCTATGCATAAAAAGCAGCGGCT‑GACGTCTTCCGACGTTGGCCGTTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTG                           <  SRR4302545.9974085/94‑1 (MQ=60)
                                                                  GTCTATGCATAAAAAGCAGCGGCT‑GACGTCTTCCGACGTTGGCCGTTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGC                          >  SRR4302545.9283137/1‑94 (MQ=60)
                                                                   TCTATGCATAAAAAGCAGCGGCT‑GACGTCTTCCGACGTTGGCCGTTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCT                         <  SRR4302545.3207486/94‑1 (MQ=60)
                                                                   TCTATGCATAAAAAGCAGCGGCT‑GACGTCTTCCGACGTTGGCCGTTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCT                         >  SRR4302545.484991/1‑94 (MQ=60)
                                                                   TCTATGCATAAAAAGCAGCGGCT‑GACGTCTTCCGACGTTGGCCGTTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCT                         >  SRR4302545.5943535/1‑94 (MQ=60)
                                                                   TCTATGCATAAAAAGCAGCGGCT‑GACGTCTTCCGACGTTGGCCGTTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCAAGCCGTTGCT                         >  SRR4302545.5138782/1‑94 (MQ=60)
                                                                    CTATGCATAAAAAGCAGCGGCT‑GACGTCTTCCGACGTTGGCCGTTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTA                        >  SRR4302545.7205267/1‑94 (MQ=60)
                                                                     TATGCATAAAAAGCAGCGGCT‑GACGTCTTCCGACGTTGGCCGTTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTAT                       <  SRR4302545.4433903/94‑1 (MQ=60)
                                                                     TATGCATAAAAAGCAGCGGCT‑GACGTCTTCCGACGTTGGCCGTTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTAT                       <  SRR4302545.6338885/94‑1 (MQ=60)
                                                                       TGCATAAAAAGCAGCGGCT‑GACGTCTTCCGACGTTGGCCGTTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGG                     <  SRR4302545.7471950/94‑1 (MQ=60)
                                                                       TGCATAAAAAGCAGCGGCT‑GACGTCTTCCGACGTTGGCCGGTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGG                     >  SRR4302545.5627241/1‑94 (MQ=60)
                                                                        GCATAAAAAGCAGCGGCT‑GACGTCTTCCGACGTTGGCCGTTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGG                    >  SRR4302545.6779994/1‑94 (MQ=60)
                                                                        GCATAAAAAGCAGCGGCT‑GACGTCTTCCGACGTTGGCCGTTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGG                    <  SRR4302545.2914313/94‑1 (MQ=60)
                                                                         CATAAAAAGCAGCGGCT‑GACGTCTTCCGACGTTGGCCGTTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGG                   <  SRR4302545.1082239/94‑1 (MQ=60)
                                                                         CATAAAAAGCAGCGGCT‑GACGTCTTCCGACGTTGGCCGTTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGG                   <  SRR4302545.6354756/94‑1 (MQ=60)
                                                                         CATAAAAAGCAGCGGCT‑GACGTCTTCCGACGTTGGCCGTTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGG                   >  SRR4302545.2910492/1‑94 (MQ=60)
                                                                          ATAAAAAACAGCGGCT‑AACGTCTTCCGACGTTGGCCGTTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGC                  <  SRR4302545.8794694/94‑1 (MQ=60)
                                                                            AAAAAGCAGCGGCT‑GACGTCTTCCGACGTTGGCCGTTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGG                >  SRR4302545.10122750/1‑94 (MQ=60)
                                                                             AAAAGCAGCGGCT‑GACGTCTTCCGACGTTGGCCGTTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGA               <  SRR4302545.4454120/94‑1 (MQ=60)
                                                                              AAAGCAGCGGCT‑GACGTCTTCCGACGTTGGCCGTTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGAT              <  SRR4302545.5855558/94‑1 (MQ=60)
                                                                                AGCAGCGGCT‑GACGTCTTCCGACGTTGGCCGTTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTC            <  SRR4302545.5591837/94‑1 (MQ=60)
                                                                                AGCAGCGGCT‑GACGTCTTCCGACGTTGGCCGTTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTC            <  SRR4302545.5231196/94‑1 (MQ=60)
                                                                                AGCAGCGGCT‑GACGTCTTCCGACGTTGGCCGTTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTC            <  SRR4302545.2377136/94‑1 (MQ=60)
                                                                                AGCAGCGGCT‑GACGTCTTCCGACGTTGGCCGTTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTC            <  SRR4302545.139061/94‑1 (MQ=60)
                                                                                 GCAGCGGCT‑GACGTCTTCCGACGTTGGCCGTTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCG           <  SRR4302545.2986654/94‑1 (MQ=60)
                                                                                 GCAGCGGCT‑GACGTCTTCCGACGTTGGCCGTTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCG           >  SRR4302545.7173400/1‑94 (MQ=60)
                                                                                  CAGCGGCT‑GACGTCTTCCGACGTTGGCCGTTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGC          >  SRR4302545.7910360/1‑94 (MQ=60)
                                                                                   AGCGGCT‑GACGTCTTCCGACGTTGGCCGTTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCT         <  SRR4302545.6741612/94‑1 (MQ=60)
                                                                                    GCGGCT‑GACGTCTTCCGACGTTGGCCGTTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTG        <  SRR4302545.2195430/94‑1 (MQ=60)
                                                                                    GCGGCT‑GACGTCTTCCGACGTTGGCCGTTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTG        <  SRR4302545.7297914/94‑1 (MQ=60)
                                                                                    GCGGCT‑GACGTCTTCCGACGTTGGCCGTTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTG        <  SRR4302545.10396164/94‑1 (MQ=60)
                                                                                    GCGGCT‑GACGTCTTCCGACGTTGGCCGTTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTG        <  SRR4302545.10396163/94‑1 (MQ=60)
                                                                                     CGGCT‑GACGTCTTCCGACGTTGGCCGTTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGT       <  SRR4302545.6147166/94‑1 (MQ=60)
                                                                                     CGGCT‑GACGTCTTCCGACGTTGGCCGTTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGT       >  SRR4302545.10883187/1‑94 (MQ=60)
                                                                                       GCT‑GACGTCTTCCGACGTTGGCCGTTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTG     <  SRR4302545.9252184/94‑1 (MQ=60)
                                                                                         ctGACGTCTTCCGACGTTGGCCGTTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGT    <  SRR4302545.586327/92‑1 (MQ=60)
                                                                                          TGACGTCTTCCGACGTTGGCCGTTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTT   <  SRR4302545.313260/94‑1 (MQ=60)
                                                                                          TGACGTCTTCCGACGTTGGCCGTTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTT   >  SRR4302545.9367295/1‑94 (MQ=60)
                                                                                          TGACGTCTTCCGACGTTGGCCGTTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTT   <  SRR4302545.5595197/94‑1 (MQ=60)
                                                                                          TGACGTCTTCCGACGTTGGCCGTTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTT   <  SRR4302545.280941/94‑1 (MQ=60)
                                                                                           GACGTCTTCCGACGTTGGCCGTTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTG  >  SRR4302545.4494936/1‑94 (MQ=60)
                                                                                           GACGTCTTCCGACGTTGGCCGTTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTG  <  SRR4302545.1716804/94‑1 (MQ=60)
                                                                                           GACGTCTTCCGACGTTGGCCGTTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTG  >  SRR4302545.8075118/1‑94 (MQ=60)
                                                                                           |                                                                                             
CAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGGACGTCTTCCGACGTTGGCCGTTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTG  >  NC_000913/3236086‑3236270

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑