Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 647 270.1 21258240 98.4% 20918108 94.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,001,486 2 bp→GC coding (316‑317/903 nt) rarD ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,001,4860CG99.8% 2014.2 / ‑4.8 579R106A (CGG→GCG) rarDhypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base G (277/301);  total (278/301)
Fisher's exact test for biased strand distribution p-value = 4.80e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.43e-01
*NC_0009134,001,4870GC99.8% 2020.1 / ‑5.3 579R106A (CGG→GCG) rarDhypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base C (277/300);  total (278/301)
Fisher's exact test for biased strand distribution p-value = 4.81e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01

Only 100 of 590 total aligned reads displayed.

CAGCCATTGCATCCGGCGGAATCCGTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCCGTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGCACGGCAGAGACTGCCAGCATAAAAAT  >  NC_000913/4001398‑4001574
                                                                                        ||                                                                                       
cagccaTTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTcc                                                                                     <  1:6299886/94‑1 (MQ=255)
cagccaTTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTcc                                                                                     <  1:2356005/94‑1 (MQ=255)
 agccaTTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCa                                                                                    <  2:8498246/94‑1 (MQ=255)
  gccaTTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAg                                                                                   <  1:26410/94‑1 (MQ=255)
  gccaTTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAg                                                                                   <  2:306721/94‑1 (MQ=255)
  gccaTTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAg                                                                                   <  2:2491581/94‑1 (MQ=255)
     aTTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCat                                                                                <  1:7534418/94‑1 (MQ=255)
     aTTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCat                                                                                >  2:1124534/1‑94 (MQ=255)
       tGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCatat                                                                              <  1:2968443/94‑1 (MQ=255)
          aTCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGt                                                                           <  1:8296994/94‑1 (MQ=255)
          aTCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGt                                                                           <  1:571197/94‑1 (MQ=255)
           tCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTg                                                                          >  1:5199159/1‑94 (MQ=255)
            ccGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGa                                                                         >  2:5064130/1‑94 (MQ=255)
              ggcggAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGAtt                                                                       >  2:4720277/1‑94 (MQ=255)
               gcggAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCT‑CGCTTCAGCATATGGTGATTGt                                                                     >  1:7906900/1‑94 (MQ=255)
                cggAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGt                                                                     <  1:9882329/94‑1 (MQ=255)
                 ggAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGtt                                                                    <  1:4638533/94‑1 (MQ=255)
                  gAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTc                                                                   <  2:7502526/94‑1 (MQ=255)
                  gAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTc                                                                   <  2:3628798/94‑1 (MQ=255)
                     tcgctCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCAcc                                                                <  1:3435185/90‑1 (MQ=255)
                     tcgctCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCAcc                                                                >  2:7729725/5‑94 (MQ=255)
                      cgctCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCg                                                               >  1:4559621/4‑94 (MQ=255)
                      cgctCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCg                                                               <  2:1363114/91‑1 (MQ=255)
                      cgctCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCg                                                               <  1:3789384/91‑1 (MQ=255)
                       gctCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGTTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGc                                                              <  2:2913816/92‑1 (MQ=255)
                       gctCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGc                                                              <  2:10563861/92‑1 (MQ=255)
                       gctCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGc                                                              >  1:2041502/3‑94 (MQ=255)
                          cGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCa                                                           <  1:2847540/94‑1 (MQ=255)
                            ccGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGa                                                         >  1:4296333/1‑94 (MQ=255)
                             cGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGAt                                                        <  1:6536530/94‑1 (MQ=255)
                                  aaaaTCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACa                                                   >  2:8610565/1‑94 (MQ=255)
                                  aaaaTCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACa                                                   <  2:4447300/94‑1 (MQ=255)
                                  aaaaTCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACa                                                   <  2:1856241/94‑1 (MQ=255)
                                   aaaTCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAg                                                  <  2:5383405/94‑1 (MQ=255)
                                    aaTCATCCCCAGCACAATGTTCACCCGCGGGTTAATAAAGTAACCCAGGCTCGGCTCCAGCATATGGTGATTGTTCACCGCCCAGATACACAga                                                 >  2:6070625/1‑93 (MQ=255)
                                      tcatcCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAg                                               <  2:5681314/94‑1 (MQ=255)
                                      acatcCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAg                                               <  2:3568027/93‑1 (MQ=255)
                                        atcCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGcc                                             >  1:155076/1‑94 (MQ=255)
                                        atcCCCAGCACAAGGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGcc                                             <  2:6408228/94‑1 (MQ=255)
                                         tcCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCa                                            >  1:6874959/1‑94 (MQ=255)
                                         tcCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCa                                            <  2:8779971/94‑1 (MQ=255)
                                          ccccAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAg                                           <  2:10374174/94‑1 (MQ=255)
                                          ccccAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAg                                           <  2:2399993/94‑1 (MQ=255)
                                              aGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCTAGATAAACAGTAGCCAGTTGc                                       <  2:9021120/94‑1 (MQ=255)
                                              aGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGc                                       >  1:6053812/1‑94 (MQ=255)
                                               gCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGcc                                      <  1:8436203/94‑1 (MQ=255)
                                               gCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGcc                                      >  2:5765095/1‑94 (MQ=255)
                                               gCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGcc                                      <  1:9751294/94‑1 (MQ=255)
                                               gCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGcc                                      >  2:8685397/1‑94 (MQ=255)
                                                cacaATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGcca                                     <  1:7348878/94‑1 (MQ=255)
                                                 acaATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGccac                                    <  1:4391685/94‑1 (MQ=255)
                                                  caATGTTCACGAGCGGGTTAATAAAGTAACCGAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGccacc                                   <  2:3819810/94‑1 (MQ=255)
                                                  caATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGccacc                                   <  1:4007094/94‑1 (MQ=255)
                                                   aaTGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACTGTAGCCTGTTGccacca                                  >  2:9386347/1‑94 (MQ=255)
                                                    aTGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCaa                                 <  2:1442520/94‑1 (MQ=255)
                                                    aTGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCaa                                 >  2:6817905/1‑94 (MQ=255)
                                                     ttttCACCAGCGTGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAAt                                <  1:1349474/92‑1 (MQ=255)
                                                     tGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACGAAt                                >  1:9092149/1‑94 (MQ=255)
                                                        tCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAg                             <  1:10309361/94‑1 (MQ=255)
                                                         cACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGc                            >  2:10205557/1‑94 (MQ=255)
                                                          aCCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGCa                           >  1:1268007/1‑94 (MQ=255)
                                                           ccAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTTACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGCAc                          >  2:2084306/1‑94 (MQ=255)
                                                            cAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGCACg                         <  1:8045590/94‑1 (MQ=255)
                                                            cAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGCACg                         >  1:2105726/1‑94 (MQ=255)
                                                             aGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGCACgg                        <  1:9246391/94‑1 (MQ=255)
                                                             aGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGCACgg                        <  2:2662089/94‑1 (MQ=255)
                                                              gCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGCACGGc                       >  1:3174774/1‑94 (MQ=255)
                                                               cGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGCACGGCa                      <  1:1122354/94‑1 (MQ=255)
                                                                gggTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGCACGGCag                     <  2:9828996/94‑1 (MQ=255)
                                                                gggTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGCACGGCag                     >  1:10213835/1‑94 (MQ=255)
                                                                 ggTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGCACGGCaga                    <  2:1578404/94‑1 (MQ=255)
                                                                 ggTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGCACGGCaga                    >  2:7942558/1‑94 (MQ=255)
                                                                 ggTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGCACGGCaga                    >  1:712855/1‑94 (MQ=255)
                                                                 ggTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGCACGGCaga                    <  1:7613539/94‑1 (MQ=255)
                                                                  gTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGCACGGCagag                   <  2:3325856/94‑1 (MQ=255)
                                                                  gTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGCACGGCagag                   >  2:6291429/1‑94 (MQ=255)
                                                                    taataaAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGCACGGCAGAGAc                 <  2:7207810/94‑1 (MQ=255)
                                                                     aataaAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGCACGGCAGAGACt                <  2:401879/94‑1 (MQ=255)
                                                                     aataaAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGCACGGCAGAGACt                <  2:6623833/94‑1 (MQ=255)
                                                                     aataaAGTAACCAAGGCTCGATTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGCACGGCAGAGACt                <  1:10538612/94‑1 (MQ=255)
                                                                      ataaAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGCACGGCAGAGACTg               <  1:4958038/94‑1 (MQ=255)
                                                                      ataaAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGCACGGCAGAGACTg               >  1:8678336/1‑94 (MQ=255)
                                                                      ataaAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGGTGCCACCAATCAGCACGGCAGAGAGTg               >  1:5464974/1‑94 (MQ=255)
                                                                         aaGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGCACGGCAGAGACTGCCa            <  2:6959992/94‑1 (MQ=255)
                                                                          aGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGCACGGCAGAGACTGCCAg           <  2:4162654/94‑1 (MQ=255)
                                                                          aGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGCACGGCAGAGACTGCCAg           >  2:424051/1‑94 (MQ=255)
                                                                           gTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGCACGGCAGAGACTGCCAGc          <  2:9239279/94‑1 (MQ=255)
                                                                             aaCCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGCACGGCAGAGACTGCCAGCAt        <  1:891218/94‑1 (MQ=255)
                                                                               ccAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGCACGGCAGAGACTGCCAGCATaa      >  1:9556156/1‑94 (MQ=255)
                                                                               ccAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGCACGGCAGAGACTGCCAGCATaa      >  2:4827263/1‑94 (MQ=255)
                                                                               ccAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGCACGGCAGAGACTGCCAGCATaa      <  1:10105221/94‑1 (MQ=255)
                                                                                cAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGCACGGCAGAGACTGCCAGCATaaa     >  1:9962600/1‑94 (MQ=255)
                                                                                cAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGCACGGCAGAGACTGCCAGCATaaa     >  1:4767120/1‑94 (MQ=255)
                                                                                 aaGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGCACGGCAGAGACTGCCAGCATaaaa    >  1:8572389/1‑94 (MQ=255)
                                                                                 aaGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGCACGGCAGAGACTGCCAGCATaaaa    <  2:8265343/94‑1 (MQ=255)
                                                                                 aaGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGCACGGCAGAGACTGCCAGCATaaaa    >  2:9721444/1‑94 (MQ=255)
                                                                                 aaGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTGGCCACCAATCAGCACGGCAGAGACTGCCAGCATaaaa    >  1:1828264/1‑94 (MQ=255)
                                                                                  aGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGCACGGCAGAGACTGCCAGCATaaaaa   >  1:6650437/1‑94 (MQ=255)
                                                                                   ggCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGCACGGCAGAGACTGCCAGCATAAAAAt  <  2:7658526/94‑1 (MQ=255)
                                                                                   ggCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGCACGGCAGAGACTGCCAGCATAAAAAt  <  2:1730033/94‑1 (MQ=255)
                                                                                        ||                                                                                       
CAGCCATTGCATCCGGCGGAATCCGTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCCGTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGCACGGCAGAGACTGCCAGCATAAAAAT  >  NC_000913/4001398‑4001574

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 322 total aligned reads displayed.
ATGCTCATCAGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTA‑C‑G‑TGGCGCTAT‑ACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATCGGGAATACTCATTTTTAATT  >  NC_000913/4001624‑4001810
                                                                                               |                                                                                               
ATGCTCATCAGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTA‑C‑G                                                                                                 >  SRR4302545.9859300/1‑94 (MQ=60)
ATGCTCATCAGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTA‑C‑G                                                                                                 <  SRR4302545.3798345/94‑1 (MQ=60)
ATGCTCATCAGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTA‑C‑G                                                                                                 <  SRR4302545.9491408/94‑1 (MQ=60)
ATGCTCATCAGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTA‑C‑G                                                                                                 <  SRR4302545.10251514/94‑1 (MQ=60)
 TGCTCATCAGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTA‑C‑G‑C                                                                                               >  SRR4302545.3599578/1‑94 (MQ=60)
  GCTCATCAGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTA‑C‑Gct                                                                                               <  SRR4302545.7948516/94‑3 (MQ=60)
  GCTCATCAGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTA‑C‑Gct                                                                                               <  SRR4302545.1695774/94‑3 (MQ=60)
  GCTCATCAGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTA‑C‑Gct                                                                                               <  SRR4302545.83710/94‑3 (MQ=60)
  GCTCATCAGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTA‑C‑Gct                                                                                               <  SRR4302545.2360858/94‑3 (MQ=60)
  GCTCATCAGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTA‑C‑Gct                                                                                               >  SRR4302545.484411/1‑92 (MQ=60)
   CTCATCAGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGGACGTAGTAAATCAACTTGAAGTA‑C‑Gctg                                                                                              >  SRR4302545.4025105/1‑91 (MQ=60)
   CTCATCAGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGGACGTAGTAAAACAACTTGAAGTA‑C‑Gctg                                                                                              >  SRR4302545.7738636/1‑91 (MQ=60)
   CTCATCAGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTA‑C‑Gctg                                                                                              >  SRR4302545.8899599/1‑91 (MQ=60)
   CTCATCAGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTA‑C‑Gctg                                                                                              <  SRR4302545.5882954/94‑4 (MQ=60)
   CTCATCAGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTA‑C‑Gctg                                                                                              <  SRR4302545.5575735/94‑4 (MQ=60)
   CTCATCAGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTA‑C‑Gctg                                                                                              <  SRR4302545.2303107/94‑4 (MQ=60)
   CTCATCAGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTA‑C‑Gctg                                                                                              <  SRR4302545.1712154/94‑4 (MQ=60)
   CTCATCAGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTA‑C‑Gctg                                                                                              <  SRR4302545.8168228/94‑4 (MQ=60)
   CTCATCAGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTA‑C‑Gctg                                                                                              <  SRR4302545.8168226/94‑4 (MQ=60)
     CATCAGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGGTTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTA‑C‑GCTGGC                                                                                            <  SRR4302545.8688940/94‑1 (MQ=60)
     CATCAGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTA‑C‑GCTGGC                                                                                            <  SRR4302545.7163021/94‑1 (MQ=60)
     CATCAGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTA‑C‑GCTGGC                                                                                            <  SRR4302545.7163022/94‑1 (MQ=60)
     CATCAGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTA‑C‑GCTGGC                                                                                            <  SRR4302545.10025018/94‑1 (MQ=60)
     CATCAGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTA‑C‑GCTGGC                                                                                            >  SRR4302545.10684826/1‑94 (MQ=60)
      ATCAGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTA‑C‑GCTGGCG                                                                                           <  SRR4302545.9479056/94‑1 (MQ=60)
      ATCAGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTA‑C‑GCTGGCG                                                                                           >  SRR4302545.2543735/1‑94 (MQ=60)
      ATCAGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAAATTGAAGTA‑C‑Gctgggg                                                                                           >  SRR4302545.802878/1‑88 (MQ=60)
      ATCAGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAAATTGAAGTA‑C‑GCTGGCG                                                                                           >  SRR4302545.3460162/1‑94 (MQ=60)
       TCAGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTA‑C‑GCTGGCGC                                                                                          <  SRR4302545.2886862/94‑1 (MQ=60)
        CAGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCATGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTA‑C‑GCTGGCGCT                                                                                         <  SRR4302545.7870149/94‑1 (MQ=60)
        CAGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTA‑C‑GCTGGCGCT                                                                                         <  SRR4302545.7629839/94‑1 (MQ=60)
        CAGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTA‑C‑GCTGGCGCT                                                                                         <  SRR4302545.3024127/94‑1 (MQ=60)
         AGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTA‑C‑GCTGGCGCTA                                                                                        <  SRR4302545.3499257/94‑1 (MQ=60)
          GCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTA‑C‑GCTGGCGCTAT                                                                                       >  SRR4302545.5359725/1‑94 (MQ=60)
          GCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTA‑C‑GCTGGCGCTAT                                                                                       >  SRR4302545.10879407/1‑94 (MQ=60)
           CACCACCATCAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGGACGTGGTTCATCAACCTGAAGTA‑C‑GCTGGCGCTCT‑A                                                                                     >  SRR4302545.6135348/1‑94 (MQ=60)
           CACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCGCGTAGTAAATCAACTTGAAGTA‑C‑GCTGGCGCTAT‑A                                                                                     >  SRR4302545.9463360/1‑94 (MQ=60)
           CACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTA‑C‑GCTGGCGCTAT‑A                                                                                     <  SRR4302545.4912529/94‑1 (MQ=60)
           CACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTA‑C‑GCTGGCGCTAT‑A                                                                                     <  SRR4302545.6466413/94‑1 (MQ=60)
           CACCACCATAAAAAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTA‑C‑GCTGGCGCTAT‑A                                                                                     <  SRR4302545.8496966/94‑1 (MQ=60)
             CCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATTAACTTTAAGTA‑C‑GCTGGCGCTAT‑ACC                                                                                   >  SRR4302545.2800983/1‑94 (MQ=60)
             CCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTA‑C‑GCTGGCGCTAT‑ACC                                                                                   >  SRR4302545.3068252/1‑94 (MQ=60)
             CCACCAGAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTA‑C‑GCTGGCGCTAT‑GCC                                                                                   <  SRR4302545.8859783/94‑1 (MQ=60)
              CACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTA‑C‑GCTGGCGCTAT‑ACCC                                                                                  <  SRR4302545.7005258/94‑1 (MQ=60)
               ACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTATC‑C‑TGGCGCTATCACCC                                                                                  >  SRR4302545.3503983/1‑94 (MQ=60)
               ACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGGA‑A‑GCTGGCGCTAT‑ACCCC                                                                                 >  SRR4302545.225840/1‑94 (MQ=60)
                CCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTA‑C‑GCTGGCGCTAT‑ACCCCA                                                                                <  SRR4302545.10589681/94‑1 (MQ=60)
                CCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTA‑C‑GCTGGCGCTAT‑ACCCCA                                                                                >  SRR4302545.8237480/1‑94 (MQ=60)
                 CATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTA‑C‑GCTGGGGCTAT‑ACCCCCA                                                                               >  SRR4302545.2332459/1‑94 (MQ=60)
                 CATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTA‑C‑GCTGGCGCTAT‑ACCCCAA                                                                               >  SRR4302545.5180774/1‑94 (MQ=60)
                  ATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTA‑C‑GCTGGCGCTAT‑ACCCCAAA                                                                              <  SRR4302545.3789210/94‑1 (MQ=60)
                   AAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTA‑C‑GCTGGCGCTAT‑ACCCCAAAT                                                                             <  SRR4302545.6788195/94‑1 (MQ=60)
                    AAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTA‑C‑GCTGGCGCTAT‑ACCCCAAATA                                                                            <  SRR4302545.10464596/94‑1 (MQ=60)
                     AAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTA‑C‑GCTGGCGCTAT‑ACCCCAAATAA                                                                           >  SRR4302545.7311337/1‑94 (MQ=60)
                     AAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTA‑C‑GCTGGCGCTAT‑AACCCAAATAA                                                                           >  SRR4302545.266247/1‑94 (MQ=60)
                      AGTAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTA‑C‑GCTGGCGCTAT‑ACCCCAAATAAA                                                                          >  SRR4302545.7564780/1‑94 (MQ=60)
                      AGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTA‑C‑GCTGGCGCTAT‑ACCCCAAATAAA                                                                          <  SRR4302545.4567870/94‑1 (MQ=60)
                      AGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTA‑C‑GCTGGCGCTAT‑ACCCCAAATAAA                                                                          <  SRR4302545.4789983/94‑1 (MQ=60)
                      AGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGGAAATCAACTTGAAGTA‑T‑GCTGGCGCTAT‑ACCCCAAATAAA                                                                          >  SRR4302545.7393553/1‑94 (MQ=60)
                        AAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAAGTTGAAGTA‑C‑GCTGGCGCTAT‑ACCCCAAATAAAAT                                                                        >  SRR4302545.9044176/1‑94 (MQ=60)
                        AAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGCAGTA‑C‑GCTAGCGCTAT‑ACCCCCAATAAAAA                                                                        >  SRR4302545.3937328/1‑94 (MQ=60)
                        AAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTA‑C‑GCTGGCGCTAT‑ACCCCAAATAAAAA                                                                        >  SRR4302545.6746477/1‑94 (MQ=60)
                        AAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACATGAAGTA‑C‑GATGGCGCTAT‑ACCCCAAATAAAAT                                                                        >  SRR4302545.9563718/1‑94 (MQ=60)
                         AAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTA‑C‑GCTGGCGCTAT‑ACCCCAAATAAAATA                                                                       >  SRR4302545.7117442/1‑94 (MQ=60)
                          AAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGTGCACGTAGTAAATCAACTTGAAGTA‑C‑GCTGGCGCTAT‑ACCCCAAATAAAATAA                                                                      <  SRR4302545.9910333/94‑1 (MQ=60)
                          AAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTA‑C‑GCTGGCGCTAT‑ACCCCAAATAAAATAA                                                                      <  SRR4302545.5210275/94‑1 (MQ=60)
                          AAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTA‑C‑GCTGGCGCTAT‑ACCCCAAATAAAATAA                                                                      <  SRR4302545.599838/94‑1 (MQ=60)
                          AAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTA‑C‑GCTGGCGCTAT‑ACCCCAAATAAAATAA                                                                      <  SRR4302545.4466963/94‑1 (MQ=60)
                           AACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTA‑C‑GCTGGCGCTAT‑ACCCCAAATAAAATAAG                                                                     <  SRR4302545.50303/94‑1 (MQ=60)
                           AACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTA‑C‑GCTGGCGCTAT‑ACCCCAAATAAAATAAG                                                                     <  SRR4302545.3862489/94‑1 (MQ=60)
                            ACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTA‑C‑GCTGGCGCTAT‑ACCCCAAATAAAATAAGC                                                                    >  SRR4302545.3592077/1‑94 (MQ=60)
                             CGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTA‑C‑GCTTGCGCTAT‑ACCCCAAATAAAATAAGCG                                                                   >  SRR4302545.9637881/1‑94 (MQ=60)
                              GACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTA‑C‑GCTGGCGCTAT‑ACCCCAAATAAAATAAGCGG                                                                  <  SRR4302545.10010981/94‑1 (MQ=60)
                              GACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTA‑C‑GCTGGCGCTAT‑ACCCCAAATAAAATAAGCGG                                                                  >  SRR4302545.5256625/1‑94 (MQ=60)
                              GACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAAATTGAATTA‑C‑GCTTGCGCTAT‑ACCCCAAATAAAAaaaacag                                                                  >  SRR4302545.8105674/1‑87 (MQ=60)
                               ACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTTAAGTA‑C‑GCTGGCGCTAT‑ACCCCAAATAAAATAAGCCGC                                                                 >  SRR4302545.4952700/1‑94 (MQ=60)
                               ACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTA‑C‑GCTGGCGCTAT‑ACCCCAAATAAAATAAGCGGC                                                                 <  SRR4302545.1016557/94‑1 (MQ=60)
                               ACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTA‑C‑GCTGGCGCTAT‑ACCCCAAATAAAATAAGCGGC                                                                 >  SRR4302545.315706/1‑94 (MQ=60)
                               ACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTA‑C‑GCTGGCGCTAT‑ACCCCAAATAAAATAAGCGGC                                                                 >  SRR4302545.10172768/1‑94 (MQ=60)
                                CCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTA‑C‑GCTGGCGCTAT‑ACCCCAAATAAAATAAGCGGCA                                                                >  SRR4302545.2365457/1‑94 (MQ=60)
                                CCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTA‑C‑GCTGGCGCTAT‑ACCCCAAATAAAATAAGCGGCA                                                                >  SRR4302545.10076958/1‑94 (MQ=60)
                                 CAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTA‑C‑GCTGGGGCTAT‑AGCCCAAATAAAATAAACGGCAA                                                               >  SRR4302545.1850018/1‑94 (MQ=60)
                                 CAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTA‑C‑GCTGGCGCTAT‑ACCCCAAATAAAATAAGCGGCAA                                                               >  SRR4302545.7252735/1‑94 (MQ=60)
                                 CAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTA‑C‑GCTGGCGCTAT‑ACCCCAAATAAAATAAGCGGCAA                                                               <  SRR4302545.5377124/94‑1 (MQ=60)
                                 CAGATCACGCGATGCGTCAGGATTTCATCGGCGGGAACGTAGTAAATCAACTTGAAATA‑C‑GCTGGCGCTAT‑ACCCCAAATAAAAAAAGCGGCAA                                                               >  SRR4302545.3175918/1‑94 (MQ=60)
                                  AGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTA‑C‑GCTGGCGCTAT‑ACCCCAAATAAAATAAGCGGCAAG                                                              <  SRR4302545.4085346/94‑1 (MQ=60)
                                  AGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTA‑C‑GCTGGCGCTAT‑ACCCCAAATAAAATAAGCGGCAAG                                                              <  SRR4302545.3004186/94‑1 (MQ=60)
                                    ATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTA‑C‑GCTGGCGCTAT‑ACCCCAAATAAAATAAGCGGCAAGAG                                                            <  SRR4302545.7079858/94‑1 (MQ=60)
                                    ATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTA‑C‑GCTGGCGCTAT‑ACCCCAAATAAAATAAGCGGCAAGAG                                                            <  SRR4302545.4340834/94‑1 (MQ=60)
                                    ATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTA‑C‑GCTGGCGCTAT‑ACCCCAAATAAAATAAGCGGCAAGAG                                                            <  SRR4302545.3680621/94‑1 (MQ=60)
                                    ATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTA‑C‑GCTGGCGCTAT‑ACCCCAAATAAAATAAGCGGCAAGAG                                                            >  SRR4302545.9901918/1‑94 (MQ=60)
                                    ATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGGAAATCAACTTGAAGTA‑C‑GCTGGCGCTAT‑ACCCCAAATAAAATAAGCGGCAAGAG                                                            >  SRR4302545.9901917/1‑94 (MQ=60)
                                      CACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTA‑C‑GCTGGCGCTAT‑ACCCCAAATAAAATAAGCGGCAAGAGCG                                                          <  SRR4302545.3053302/94‑1 (MQ=60)
                                      CACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGGAAATCAACTTGAAGTA‑C‑GCTGGGGCTAT‑ACCCCAAATAAAATAAGCGGCCAGAGCG                                                          >  SRR4302545.3248541/1‑94 (MQ=60)
                                       ACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTA‑C‑GCTGGCGCTAT‑ACCCCAAATAAAATAAGCGGCAAGAGCGA                                                         <  SRR4302545.5653327/94‑1 (MQ=60)
                                        CGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTA‑C‑GCTGGTGCTAT‑ACCCCAAATAAAATAAGCGGCAAGAGCGAG                                                        <  SRR4302545.291357/94‑1 (MQ=60)
                                        CGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTA‑C‑GCTGGCGCTAT‑ACCCCAAATAAAATAAGCGGCAAGAGCGAG                                                        >  SRR4302545.2271470/1‑94 (MQ=60)
                                        CGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAAATTGAAGTA‑C‑GCTGGCGCTAT‑ACCCCAAATAAAATAAGCGGCAAGAGCGAG                                                        >  SRR4302545.5582133/1‑94 (MQ=60)
                                         GCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTA‑C‑GCTGGCGCTAT‑ACCCCAAATAAAATAAGCGGCAAGAGCGAGA                                                       >  SRR4302545.280590/1‑94 (MQ=60)
                                         GCGATGCGTCAGAATATCATCGGCGGACACGTAGTAAATCAACTTGAAGTA‑C‑GCTGGCGCTAT‑ACCCCAAATAAAAAAAGCGGCAAGAGCGAGT                                                       <  SRR4302545.9699404/94‑1 (MQ=60)
                                          CGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTA‑C‑GCTGGCGCTAT‑ACagatcagagatcggaagagcgtcgtgtagg                                                      >  SRR4302545.1031157/1‑64 (MQ=60)
                                          CGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTA‑C‑GCTGGCGCTAT‑ACCCCAAATAAAATAAGCGGCAAGAGCGAGTA                                                      <  SRR4302545.5101179/94‑1 (MQ=60)
                                           GATGCGTGAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTA‑C‑GCTGGCGCTAT‑ACCCCAAATAAAATAAGCGGCAAGAGCGAGTAA                                                     >  SRR4302545.10730487/1‑94 (MQ=60)
                                           GATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTA‑C‑GCTGGCGCTAT‑ACCCCAAATAAAATAAGCGGCAAGAGCGAGTAA                                                     <  SRR4302545.790602/94‑1 (MQ=60)
                                            ATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTA‑C‑GCTGGCGCTAT‑ACCCCAAATAAAATAAGCGGCAAGAGCGAGTAAT                                                    <  SRR4302545.1618560/94‑1 (MQ=60)
                                            ATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTA‑C‑GCTGGCGCTAT‑ACCCCAAATAAAATAAGCGGCAAGAGCGAGTAAT                                                    >  SRR4302545.1982977/1‑94 (MQ=60)
                                            ATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTA‑C‑GCTGGCGCTAT‑ACCCCAAATAAAATAAGCGGCAAGAGCGAGTAAA                                                    >  SRR4302545.8386477/1‑94 (MQ=60)
                                             TGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTA‑C‑GCTGGCGCTAT‑ACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATA                                                   >  SRR4302545.1285590/1‑94 (MQ=60)
                                              GCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTA‑C‑GCTGGCGCTAT‑ACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATAC                                                  <  SRR4302545.9250695/94‑1 (MQ=60)
                                               CGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTA‑C‑GCTGGCGCTAT‑ACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACG                                                 >  SRR4302545.9513041/1‑94 (MQ=60)
                                               CGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTA‑C‑GCTGGCGCTAT‑ACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACG                                                 >  SRR4302545.3294223/1‑94 (MQ=60)
                                                GTCAGGTTTCCATCGGCGTGCACGTTGTAAATCAACTTGAAGTA‑CTC‑TGGCGCTAT‑ACTCCTAATAAAATAAGCTGCATGAGCGAGTAAAACGC                                                >  SRR4302545.9664433/1‑94 (MQ=26)
                                                GTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTA‑C‑GCTGGCGCTAT‑ACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGC                                                >  SRR4302545.350423/1‑94 (MQ=60)
                                                GTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTA‑C‑GCTGGCGCTAT‑ACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGC                                                >  SRR4302545.9164823/1‑94 (MQ=60)
                                                 TCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTA‑C‑GCTGGCGCTAT‑ACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCC                                               >  SRR4302545.3929162/1‑94 (MQ=60)
                                                 TCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTA‑C‑GCTGGCGCTAT‑ACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCC                                               <  SRR4302545.9064348/94‑1 (MQ=60)
                                                   AGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTA‑C‑GCTGGCGCTAT‑ACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCT                                             <  SRR4302545.751419/94‑1 (MQ=60)
                                                    GGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTC‑C‑GCTGGCGCTAT‑ACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTG                                            <  SRR4302545.5875211/94‑1 (MQ=60)
                                                     GATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTA‑C‑GCTGGCGCTAT‑ACCCCAAATAAAATAAGCGGCAgcgaacttcaagttgatttac                                           <  SRR4302545.10830350/94‑22 (MQ=60)
                                                     GATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTA‑C‑GCTGGCGCTAT‑ACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGC                                           <  SRR4302545.8081835/94‑1 (MQ=60)
                                                     GATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTA‑C‑GCTGGCGCTAT‑ACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGC                                           >  SRR4302545.5484123/1‑94 (MQ=60)
                                                     GATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTA‑C‑GCTGGCGCTAT‑ACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGC                                           <  SRR4302545.4791652/94‑1 (MQ=60)
                                                      ATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTA‑C‑GCTGGCGCTAT‑ACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCC                                          <  SRR4302545.4293920/94‑1 (MQ=60)
                                                      ATTTCATCGGCGGGCACGTAGGAAATCAACTTGAAGGA‑C‑GCTGGCGCTAT‑ACCCCAAATAAAATAAGCGGCAAGAGCGAGAAAAACGCCCTGCC                                          >  SRR4302545.9319619/1‑94 (MQ=60)
                                                       TTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTA‑C‑GCTGGCGCTAT‑ACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCG                                         <  SRR4302545.4204518/94‑1 (MQ=60)
                                                         TCATCGGCGGGCACGTAGTAAATCAACTTGAAGTA‑C‑GCTGGCGCTAT‑ACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCG                                       <  SRR4302545.10225250/94‑1 (MQ=60)
                                                         TCATCGGCGGGCACGTAGTAAATCAACTTGAAGTA‑C‑GCTGGCGCTAT‑ACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCG                                       >  SRR4302545.1889966/1‑94 (MQ=60)
                                                          CATCGGCGGGCACGTAGTAAATCAACTTGAAGTA‑C‑GCTGGCGCTAT‑ACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGT                                      >  SRR4302545.9312473/1‑94 (MQ=60)
                                                          CATCGGCGGGCACGTAGTAAATCAACTTGAAGTA‑C‑GCTGGCGCTAT‑ACCCCAAATAAAATAAGCGGCAAGAGCGAGCAATACGCCCTGCCGCGT                                      >  SRR4302545.9312474/1‑94 (MQ=60)
                                                           ATCGGCGGGCACGTAGTAAATCAACTTGAAGTA‑C‑GCTGGCGCTAT‑ACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTT                                     >  SRR4302545.7852185/1‑94 (MQ=60)
                                                           ATCGGCGGGCACGTAGTAAATCAACTTGAAGTA‑C‑GCTGGCGCTAT‑ACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTT                                     >  SRR4302545.988484/1‑94 (MQ=60)
                                                            TCGGCGGGCACGTAGTAAATCAACTTGAAGTA‑C‑GCTGGCGCTAT‑ACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTT                                    <  SRR4302545.7232215/94‑1 (MQ=60)
                                                            TCGGCGGGCACGTAGTAAATCAACTTGAAGTA‑C‑GCTGGCGCTAT‑ACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTT                                    <  SRR4302545.6563475/94‑1 (MQ=60)
                                                            TCGGCGGGCACGTAGTAAATCAACTTGAAGTA‑C‑GCTGGCGCTAT‑ACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTT                                    <  SRR4302545.936076/94‑1 (MQ=60)
                                                            TCGGCGGGCACGTAGTAAATCAACTTGAAGTA‑C‑GCTGGCGCTAT‑ACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTT                                    <  SRR4302545.9366316/94‑1 (MQ=60)
                                                             CGGCGGGCACGTAGTAAATCAACTTGAAGTA‑C‑GCTGGCGCTAT‑ACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTG                                   <  SRR4302545.10583634/94‑1 (MQ=60)
                                                             CGGCGGGCACGTAGTAAATCAACTTGAAGTA‑C‑GCTGGCGCTAT‑ACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTG                                   >  SRR4302545.3703994/1‑94 (MQ=60)
                                                              GGCGGGCACGTAGTAAATCAACTTGAAGTA‑C‑GCTGGCGCTAT‑ACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGT                                  <  SRR4302545.2404885/94‑1 (MQ=60)
                                                              GGCGGGCACGTAGTAAATCAACTTGAAGTA‑C‑GCTGGCGCTAT‑ACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGT                                  <  SRR4302545.2674504/94‑1 (MQ=60)
                                                              GGCGGGCACGTAGTAAATCAACTTGAAGTA‑C‑GCTGGCGCTAT‑ACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGGTTGT                                  >  SRR4302545.10429522/1‑94 (MQ=60)
                                                               GCGGGCACGTAGTAAATCAACTTGAAGTA‑C‑GCTGGCGCTAT‑ACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCTCTGCCGCGTTTGTT                                 <  SRR4302545.9050191/94‑1 (MQ=60)
                                                               GCGGGCACGTAGTAAATCAACTTGAAGTA‑C‑GCTGGCGCTAT‑ACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTT                                 <  SRR4302545.282632/94‑1 (MQ=60)
                                                               GCGGGCACGTAGTAAATCAACTTGAAGTA‑C‑GCTGGCGCTAT‑ACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTT                                 >  SRR4302545.4475323/1‑94 (MQ=60)
                                                               GCGGGCACGTAATAAATCAAGTTGAGGTA‑C‑GCGGGCGCTAA‑ACCCTAAATAAAAGAAGCGGCAAGATCGAGTAATACGCCCTGCCGCGTTTGTT                                 <  SRR4302545.8740362/94‑1 (MQ=46)
                                                                CGGGCACGTAGTAAATCAACTTGAAGTA‑C‑GCTGGCGCTAT‑ACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTT                                >  SRR4302545.7685423/1‑94 (MQ=60)
                                                                CGGGCACGTAGTAAATCAACTTGAAGTA‑C‑GCTGGCGCTAT‑ACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTT                                >  SRR4302545.1017605/1‑94 (MQ=60)
                                                                CGGGCACGTAGTAAATCAACTTGAAGTA‑C‑GCTGGCGCTAT‑ACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTT                                >  SRR4302545.5797200/1‑94 (MQ=60)
                                                                CGGGCACGTAGTAAATCAACTTGAAGTA‑C‑GCTGGCGCTAT‑ACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTT                                >  SRR4302545.794905/1‑94 (MQ=60)
                                                                CGGGCACGTAGTAAATCAACTTGAAGTA‑C‑GCTGGCGCTAT‑ACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTT                                >  SRR4302545.6906463/1‑94 (MQ=60)
                                                                 GGGCACGTAGTAAATCAACTTGAAGTA‑C‑GCTGGCGCTAT‑ACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTT                               >  SRR4302545.1588332/1‑94 (MQ=60)
                                                                 GGGCACGTAGTAAATCAACTTGAAGTA‑C‑GCTGGCGCTAT‑ACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTT                               <  SRR4302545.10507004/94‑1 (MQ=60)
                                                                 GGGCACGTAGTAAATCAACTTGAAGTA‑C‑GCTGGCGCTAT‑ACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTT                               <  SRR4302545.8956936/94‑1 (MQ=60)
                                                                  GGCACGTAGTAAATCAACTTGAAGTA‑C‑GCTGGCGCTAT‑ACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTG                              >  SRR4302545.3940078/1‑94 (MQ=60)
                                                                   GCACGTAGTAAATCAACTTGAAGTA‑C‑GCTGGCGCTAT‑CCCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGC                             <  SRR4302545.9493141/94‑1 (MQ=60)
                                                                    CACGTAGTAAATCAACTTGAAGTA‑C‑GCTGGCGCTAT‑ACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCA                            <  SRR4302545.2898415/94‑1 (MQ=60)
                                                                     ACGTAGTAAATCAACTTGAAGTA‑C‑GCTGGCGCTAT‑ACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCAT                           <  SRR4302545.7064927/94‑1 (MQ=60)
                                                                     ACATAGTAAATCAACTTGAAGTA‑C‑GCTGGCGCTAT‑ACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCAT                           <  SRR4302545.4584376/94‑1 (MQ=60)
                                                                      CGTAGTAAATCAACTTGAAGTA‑C‑GCTGGCGCTAT‑ACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATC                          <  SRR4302545.970367/94‑1 (MQ=60)
                                                                      CGTAGTAAATCAACTTGAAGTA‑C‑GCTGGCGCTAT‑ACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATC                          >  SRR4302545.3001151/1‑94 (MQ=60)
                                                                       GTTGTAAATCAACTTGAAGTA‑C‑GCTGGCGCTAT‑ACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCC                         >  SRR4302545.8703834/1‑94 (MQ=60)
                                                                       GTAGTAAATCAACTTGAAGTA‑C‑GCTGGCGCTAT‑ACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCC                         <  SRR4302545.7390302/94‑1 (MQ=60)
                                                                        TAGTAAATCAACTTGAAGTA‑C‑GCTGGCGCTAT‑ACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCA                        <  SRR4302545.6215610/94‑1 (MQ=60)
                                                                        TAGTAAATCAACTTGAAGTA‑C‑GCTGGCGCTAT‑ACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCA                        >  SRR4302545.633407/1‑94 (MQ=60)
                                                                        TAGTAAATCAACTTGAAGTA‑C‑GCTGGCGCTAT‑ACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCA                        <  SRR4302545.2954824/94‑1 (MQ=60)
                                                                        TAGTAAATCAACTTGAAGTA‑C‑GCTGGCGCTAT‑ACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCA                        <  SRR4302545.788574/94‑1 (MQ=60)
                                                                        TAGTAAATCAACTTGAAGTA‑C‑GCTGGCGCTAT‑ACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCA                        <  SRR4302545.6240174/94‑1 (MQ=60)
                                                                        TAGTAAATCAACTTGAAGTA‑C‑GCTGGCGCTAT‑ACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCA                        >  SRR4302545.5430771/1‑94 (MQ=60)
                                                                         AGTAAATCAACTTGAAGTA‑C‑GCTGGCGCTAT‑ACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCAT                       >  SRR4302545.3570540/1‑94 (MQ=60)
                                                                         AGTAAATCAACTTGAAGTA‑C‑GCTGGCGCTAT‑ACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCAT                       >  SRR4302545.10279873/1‑94 (MQ=60)
                                                                         AGTAAATCAACTTGAAGTA‑C‑GCTGGCGCTAT‑ACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGGTTGTTTTGCATCCAT                       >  SRR4302545.4072238/1‑94 (MQ=60)
                                                                          GTAAATCAACTTGAAGTA‑C‑GCTGGCGCTAT‑ACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATC                      >  SRR4302545.2468882/1‑94 (MQ=60)
                                                                           TAAATCAACTTGAAGTA‑C‑GCTGGCGCTAT‑ACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATCG                     <  SRR4302545.8745671/94‑1 (MQ=60)
                                                                            AAATCAACTTGAAGTA‑C‑GCTGGCGCTAT‑ACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATCGG                    <  SRR4302545.672995/94‑1 (MQ=60)
                                                                            AAATCAACTTGAAGTA‑C‑GCTGGCGCTAT‑ACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATCGG                    >  SRR4302545.8628085/1‑94 (MQ=60)
                                                                            AAATCAACTTGAAGTA‑C‑GCTGGCGCTAT‑ACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATCGG                    <  SRR4302545.255728/94‑1 (MQ=60)
                                                                             AATCAACTTGAAGTA‑C‑GCTGGCGCTAT‑CCCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATCGGG                   <  SRR4302545.1566774/94‑1 (MQ=60)
                                                                             AATCAACTTGAAGTA‑C‑GCTGGCGCTAT‑ACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATCGGG                   <  SRR4302545.8452294/94‑1 (MQ=60)
                                                                             AATCAACTTGAAGTA‑C‑GCTGGCGCTAT‑ACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATCGCG                   >  SRR4302545.6325282/1‑94 (MQ=60)
                                                                              ATCAACTTGAAGTA‑C‑GCTGGCGCTAT‑ACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATCGGGA                  <  SRR4302545.9797928/94‑1 (MQ=60)
                                                                               TCAACTTGAAGTA‑C‑GCTGGCGCTAT‑ACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATCGGGAA                 >  SRR4302545.1416703/1‑94 (MQ=60)
                                                                               TCAACTTGAAGTA‑C‑GCTGGCGCTAT‑ACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATCGGGAA                 >  SRR4302545.1416704/1‑94 (MQ=60)
                                                                                CAACTTGAAGTA‑C‑GCTGGCGCTAT‑ACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATCGGGAAT                >  SRR4302545.6299915/1‑94 (MQ=60)
                                                                                CAACTTGAAGTA‑C‑GCTGGCGCTAT‑ACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATCGGGAAT                >  SRR4302545.2568502/1‑94 (MQ=60)
                                                                                 AACTTGAAGTA‑C‑GCTGGCGCTAT‑ACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATCGGGAATA               >  SRR4302545.5459379/1‑94 (MQ=60)
                                                                                 AACTTGAAGTA‑C‑GCTGGCGCTAT‑ACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATCGGGAATA               >  SRR4302545.3062693/1‑94 (MQ=60)
                                                                                   CTTGAAGTA‑C‑GCTGGCGCTAT‑ACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATCGGGAATACT             >  SRR4302545.3943693/1‑94 (MQ=60)
                                                                                   CTTGAAGTA‑C‑GCTGGCGCTAT‑ACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATCGGGAATACT             >  SRR4302545.7553709/1‑94 (MQ=60)
                                                                                   CTTGAAGTA‑C‑GCTGGCGCTAT‑ACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATCGGGAATACT             <  SRR4302545.8833575/94‑1 (MQ=60)
                                                                                   CTTGAAGTA‑C‑GCTGGCGCTAT‑ACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATCGGGAATAAT             >  SRR4302545.7773726/1‑94 (MQ=60)
                                                                                       AAGTA‑C‑GCTGGCGCTAT‑ACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATCGGGAATACTCATT         <  SRR4302545.10645951/94‑1 (MQ=60)
                                                                                        AGTA‑C‑GCTGGCGCTAT‑ACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATCGGGAATACTCATTT        <  SRR4302545.6289730/94‑1 (MQ=60)
                                                                                        AGTA‑C‑GCTGGCGCTAT‑ACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATCGGGAATACTCATTT        <  SRR4302545.6400236/94‑1 (MQ=60)
                                                                                         GTA‑C‑GCTGGCGCTAT‑ACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATCGGGAATACTCATTTT       >  SRR4302545.4680974/1‑94 (MQ=60)
                                                                                         GTA‑C‑GCTGGCGCTAT‑ACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATCGGGAATACTCATTTT       <  SRR4302545.10931998/94‑1 (MQ=60)
                                                                                         GTA‑C‑GCTGGCGCTAT‑ACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATCGGGAATACTCATTTT       <  SRR4302545.8884152/94‑1 (MQ=60)
                                                                                          TA‑C‑GCTGGCGCTAT‑ACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATCGGGAATACTCATTTTT      <  SRR4302545.2049438/94‑1 (MQ=60)
                                                                                               C‑TGGCGCTAT‑ACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATCGGGAATACTCATTTTTAATT  >  SRR4302545.10039611/1‑94 (MQ=60)
                                                                                               C‑TGGCGCTAT‑ACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATCGGGAATACTCATTTTTAATT  <  SRR4302545.1862653/94‑1 (MQ=60)
                                                                                               C‑TGGCGCTAT‑ACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATCGGGAATACTCATTTTTAATT  >  SRR4302545.8896910/1‑94 (MQ=60)
                                                                                               C‑TGGCGCTAT‑ACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATCGGGAATACTCATTTTTAATT  >  SRR4302545.5820486/1‑94 (MQ=60)
                                                                                               |                                                                                               
ATGCTCATCAGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTA‑C‑G‑TGGCGCTAT‑ACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATCGGGAATACTCATTTTTAATT  >  NC_000913/4001624‑4001810

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑